19 results about "Positive control" patented technology

The invention discloses a specific primer group, a kit and a method for detecting double photosynthetic bacteria, and relates to the technical field of microbiological detection. The specific primer group is designed based on a conserved region of a photosynthetic reaction center L subunit gene (pufL) of rhodopseudomonas and ectothiorhodospirillum, the conservative property is greater than or equal to 99%, the similarity rate is greater than or equal to 98.5%, specific amplification of target bacteria can be realized, and cross amplification interference is avoided. The matched kit comprises the primer group, 2 * SYBR Green qPCR Master Mix, a positive control component, a negative control component and the like, and the detection method realizes rapid and sensitive detection of the two photosynthetic bacteria by optimizing sample pretreatment, PCR amplification conditions and result interpretation standards. The detection specificity is high, the sensitivity is high (the lowest detection limit reaches 10 < 2 > cfu / mL level), the detection period is short (1-2 hours), the applicability is wide, the method can be used for detecting various samples such as river water, aquaculture water and microbial inoculum fermentation liquor, one set of primers can detect two target bacteria at the same time, the detection cost and the operation complexity are reduced, and the method is suitable for aquaculture environment monitoring and microbial inoculum application quality control.

The present disclosure relates to methods of characterizing a system comprising a chromatography column. The methods can comprise introducing a sample comprising a positive control and a negative control into a system comprising a chromatography column, wherein the positive control is a sensitive probe that interacts with the system and the negative control does not substantially interact with the system; detecting the positive control and the negative control after passing the sample through the chromatography column; and determining system suitability by comparing the amount of positive control detected to the negative control. In some embodiments, determining system suitability (e.g., sample inertness to the system) is accomplished by determining the ratio of positive control to negative control detected.

The invention discloses a control method of a ship propulsion device, the ship propulsion device and a ship, and relates to the technical field of ship propulsion. The ship propulsion device comprises a power output assembly used for ship propulsion; the manpower driving assembly is used for driving the power output assembly to rotate; the direction switching assembly is in transmission connection with the power output assembly and used for adjusting the power output direction of the power output assembly; the steering detection assembly is used for detecting the rotating direction of the manual driving assembly and outputting a forward control signal or a reverse control signal; the control method of the ship propulsion device comprises the steps that under the condition that a forward control signal is received, the direction switching assembly is controlled to drive the power output assembly to face the first direction; under the condition that the reverse control signal is received, the direction switching assembly is controlled to drive the power output assembly to face the second direction; the second direction is opposite to the first direction. The invention aims to improve the reversing stability of the ship and improve the reversing response speed of the ship at the same time.

Injection-Locked Digital Oscillator This description relates to an injection-locked digital oscillator (2). An adder (100) adds the first and second digital words (OP2, OP1) and provides a third digital word (RES) as a result, the words being N bits long, with N an integer strictly greater than 1. A register (102) updates the second word from the third word (RES) at each period of a clock signal (clk). A first circuit (200) receives a reference signal (REF) at a natural frequency of one output bit (OUT), and a reference increment, inc_ref. The first circuit calculates a first value (valref) that is selectively equal to and less than the reference increment inc_ref, depending on at least one state of the reference signal.The first circuit provides the first word at least in part by summing the first value and a positive control number, P, the output bit being a bit from the second word. Figure for the abbreviation: Fig. 2.

Such a fuel filler flap arrangement with a fuel filler flap that is mounted on an articulated arm in a limited manner and which is pivotally mounted relative to a vehicle-side filling area, wherein a mechanical positive control engages the fuel filler flap, which has at least one flexible tension member that is articulated in the area of ​​a joint axis of the articulated arm on the one hand and on the fuel filler flap on the other hand, in order to generate a relative movement of the fuel filler flap relative to the articulated arm depending on a pivoting movement of the articulated arm about the joint axis, is known. According to the invention, the articulated arm is designed as a hollow profile with an internal closed channel penetrating the articulated arm lengthwise, and the at least one tension member is routed through the channel. Use for passenger cars

The invention provides a low-voltage lithium battery collision unlocking device and a new energy vehicle, and belongs to the technical field of safety unlocking control, and the device comprises a low-voltage lithium battery module, movable and static contacts of a magnetic latching relay module and an unlocking execution mechanism which are connected in series to form a first loop; coils of the low-voltage lithium battery module, the driving circuit module and the magnetic latching relay module are connected in series to form a second loop; the collision sensor module is used for collecting a collision signal of the low-voltage lithium battery module; the MCU control unit is in communication connection with the collision sensor module and the driving circuit module and is used for responding to the collision signal and outputting a forward control signal to the driving circuit module; and the driving circuit module is used for outputting forward pulse current to the magnetic latching relay module based on the forward control signal so as to control the magnetic latching relay module to be closed. According to the invention, the technical problems of high standby power consumption, low response speed, high possibility of misoperation and high possibility of causing secondary potential safety hazards in the existing scheme can be solved.

A luggage compartment cover for a motor vehicle including a flexible cover sheet windable on and unwindable from a winding shaft mounted rotatably in a cassette housing. Opposite end regions of the cassette housing are each provided with a holder for releasable, interlocking fixing of the cassette housing in vehicle-side holding receptacles. A holder has a blocking pawl pivotable between release and blocking positions and, in a vehicle-mounted state of the cassette housing, the blocking pawl engages behind a blocking contour of a vehicle-side holding receptacle. A mechanical securing element is mounted movably between a functional position blocking the blocking pawl in the release position and an inoperative position releasing the blocking pawl for a pivoting movement. The securing element is assigned a mechanical positive control which forcibly transfers the securing element into the inoperative position when the holder of the cassette housing is inserted into the vehicle-side holding receptacle.

The invention discloses a vehicle instrument testing system and a dial switch, a power supply module, a voltage switching circuit, a communication module, a lamp and a testing method thereof, and belongs to the technical field of vehicle testing. Wherein the power supply module supplies power to the control module and the communication module; the first bit output of the dial switch controls the positive control / negative control modes of various lamps, and the state change of the various lamps is used as a visual criterion for the normal function of the instrument; the input end of the voltage switching circuit is connected with the control module, and the output end of the voltage switching circuit is connected with the voltage detection end of the instrument module; and the communication module establishes a communication link with the instrument module. The I / O interface of the dial switch is connected with various lamps at the same time, when the instrument lamps are tested, the control signals of the positive control mode or the negative control mode are input into the lamps through the dial switch, whether the lighting states of the lamps correspond to the control signals or not is observed, the various lamps in the instrument can be tested quickly, and the testing efficiency is improved. And the instrument testing efficiency is effectively improved.

The invention relates to a fixed master-slave parallel boost fast charging system for a high-speed motor. The system comprises a first battery unit and a second battery unit which are connected in parallel. The first battery unit comprises a first battery module, a parallel positive control unit, a parallel positive control relay, a parallel negative control unit and a parallel negative control relay, and the second battery unit comprises a second battery module; the parallel positive control relay is respectively connected with the parallel positive control unit, the positive electrode of the first battery module and the positive electrode of the second battery module; and the parallel negative control relay is respectively connected with the parallel negative control unit, the negative electrode of the first battery module and the negative electrode of the second battery module. According to the system, a parallel connection mode is adopted, the problem of equipment idling caused by too long charging time is solved through a boosting mode, the charging time can be greatly shortened, the use efficiency of the equipment is effectively improved, the endurance anxiety is effectively relieved, and the reliability of the battery system can be integrally improved.

Such a loading flap arrangement with a loading flap that can be moved between a closed position and an open position relative to a vehicle-mounted loading trough by means of a multi-link kinematics is known. According to the invention, a mechanical additional locking mechanism is provided between the loading flap and the loading trough, which is movably mounted between a locking position and a release position, and a mechanical positive guidance system is provided for displacing the additional locking mechanism between the locking position and the release position, which has geometric control contours on the additional locking mechanism and on the loading trough, which, depending on the relative position relative to the loading trough, effect a positive control of the additional locking mechanism in the direction of the release position or in the direction of the locking position. Suitable for passenger cars with electric charging port

The application discloses a terahertz programmable metasurface unit of an RF MEMS switch and belongs to the technical field of communication, comprising: a second substrate, a positive control line, a back cover ground, a first substrate, a direct current grounding column, an upper patch, a signal line, an RF MEMS switch and a direct current bias line structure. The switch is controlled to be turned off and turned on through direct current voltage change, and a metasurface unit 180° reflection phase difference is realized. The application achieves the following technical effects: (1) the 180° reflection phase difference working bandwidth is large, the reflection phase difference range is 172° to 193° at 285GHz to 320GHz; (2) the reflection phase is high in linearity, the reflection phase range of the switch down state is 117° to 52° at 285GHz to 320GHz, and the reflection phase range of the switch up state is -53° to -129°; (3) the processing technology requirement is low, the second layer anchor point and the lower electrode of the switch are connected through the bias line respectively, and the number of electrode punching is reduced; and (4) the bias line part uses metal, the bias line voltage division is reduced, and the driving voltage is reduced.

A method for controlling a braking force of a brake system of a vehicle by means of a control device is provided. The method comprises the following steps: if there is a positive control difference between a braking force setpoint value (Bremskraft_Set) and a braking force actual value (Bremskraft_Ist): determining a first torque setpoint value (Drehmoment_Set 1); inputting the torque setpoint value as a manipulated variable (Drehmoment_Set) into a controlled system (14) of the brake system; and adjusting the braking force on the basis of the input manipulated variable (Drehmoment_Set); if there is a negative control difference between the braking force setpoint value and the braking force actual value after a preceding positive control difference: determining a second torque setpoint value (Drehmoment_Set 2) from the control difference; calculating a compensated manipulated variable (Drehmoment_Set) by subtracting a hysteresis value (Drehmoment_Hyst) from the second torque setpoint value (Drehmoment_Set 2); inputting the compensated manipulated variable (Drehmoment_Set) as the manipulated variable into the controlled system (14) of the brake system; and adjusting the braking force on the basis of the input compensated manipulated variable (Drehmoment_Set).

The present disclosure is directed to a kit for evaluating system inertness. The kit includes a positive control comprising a metal interacting moiety, and a negative control that does not contain a metal interacting moiety. In some embodiments the kit also includes a container to hold a system suitability solution (e.g., an equimolar mixture of the positive control and the negative control).

The invention relates to an ERA-Cas12a primer, a probe, crRNA and a kit for detecting insertion of a PLCP gene into EV-G, and belongs to the technical field of animal virus molecular biology detection. The kit comprises an ERA-Cas12a primer for detecting insertion of the PLCP gene into EV-G, a probe and crRNA, and further comprises a negative control template, a positive control template, a reverse transcription and ERA amplification reagent, a double-index nucleic acid detection test strip, Cas12a protein, an FAM / Biotin reporter and a lateral flow detection test strip. The kit provided by the invention has the advantages of specificity, sensitivity, rapidness, high efficiency, isothermal amplification, on-site rapid diagnosis and the like, and is easy to popularize and apply.

The present description concerns a digital injection-locked oscillator (2). An adder (100) adds first and second digital words (OP2, OP1) and outputs a third result digital word (RES), the words being over N bits, with N an integer greater than 1. A register (102) updates the second word based on the third word (RES) at each period of a clock signal (clk). A first circuit (200) receives a reference signal (REF) at a natural frequency of an output bit (OUT), and a reference increment, inc_ref. The first circuit calculates a first value (valref) selectively equal to the reference increment inc_ref and to minus the reference increment inc_ref as a function at least of one state of the reference signal. The first circuit outputs the first word at least partly by adding the first value and a positive control number, P, the output bit being a bit of the second word.

A push-the-bit steering tool for drilling deviated wellbores comprises a steering head mountable to the bottom of a drill string and having steering pads extendable and retractable by fluid-actuated pistons; a fluid manifold co-rotatingly mounted to the steering head for delivering fluid to the pistons; a rotationally geostationary primary valve for delivering fluid to the fluid manifold; and a secondary valve for delivering fluid to the primary valve. The primary valve has a flow restrictor for regulating fluid flow to the pistons such that the steering pads will be sequentially actuated as the drill string rotates, and the steering force exerted against the wellbore by each sequentially-actuated steering pad will be in a constant selected direction. All of the pistons are hydraulically connected, thereby enabling dependent control of steering pad extension and dependent and positive control of steering pad retraction.

The present application relates to the technical field of battery management module, and more particularly to a kind of explosion fuse control end fault detection method and system.A kind of explosion fuse control end fault detection method, including first type fault detection: first detection loop is turned on, negative control end module, negative control end dry road resistance R3 and voltage division sampling unit are connected in series, explosion fuse control end equivalent resistance and parallel resistance R2 are connected in parallel with the voltage division sampling unit respectively;The voltage value V1 of detection sampling point A is calculated, the first voltage diagnostic value interval of the sampling point A under different first type fault condition is calculated, whether the voltage value V1 is in the first voltage diagnostic value interval is judged, and whether the corresponding first fault type fault of the explosion fuse control end exists is diagnosed.The type fault of positive control end and negative control end of explosion fuse can be effectively identified by using the above technical scheme, and the explosion fuse control circuit fault occurrence position is accurately positioned.

The invention relates to novel coronavirus nucleic acid detection primers and a kit, and belongs to the technical field of biological detection. The kit provided by the invention comprises a PCR (Polymerase Chain Reaction) premixed solution; positive control; performing negative control; specific primers and probes of the ORF1ab gene and the N gene of the SARS-CoV-2 virus as well as enzymes and auxiliary components required by RNA (Ribonucleic Acid) reverse transcription and DNA (Deoxyribose Nucleic Acid) amplification are covered in the PCR premixed solution. According to the kit disclosed by the invention, a one-tube PCR premixed solution, RNA reverse transcription and DNA amplification are carried out under the same system, the operation is extremely simple, convenient and time-saving, the kit can adapt to automatic detection, the transfer operation and aerosol pollution risks are effectively avoided, and the novel coronavirus RNA of various mutation types as low as 180 copies / mL can be detected within 60 minutes. The sensitivity of the kit for detecting a throat swab sample is 98.59%, and the specificity is 100%.

The utility model discloses a kit for detecting mycoplasma pneumoniae, which belongs to the technical field of biochips and diagnostic reagents, and comprises a positive quality control reagent, a negative quality control reagent and a detection chip, the detection chip comprises an upper shell and a lower shell, the upper shell is connected with the lower shell in a clamping manner, the upper shell is provided with three sample introduction windows, and the lower shell is provided with three sample introduction openings. The upper shell and the lower shell are provided with three sample introduction windows, the three sample introduction windows are respectively a negative control port, a positive control port and a detection port, and a reaction film is arranged between the upper shell and the lower shell; a negative control area is arranged at the position, opposite to the negative control opening, of the reaction film, a positive control area is arranged at the position opposite to the positive control opening, and a detection area is arranged at the position opposite to the detection opening. The kit disclosed by the utility model can accurately verify whether the quality of the detection chip is qualified or not and whether manual operation is correct or not, so that the quality control of a product is enhanced, and the accuracy of a detection result is improved.