52 results about "Solution iv" patented technology

The invention discloses a fluorescence quantitative PCR detection kit of hepatitis B virus and an application thereof. The kit is composed of the following independent components: DNA extraction solution I, DNA extraction solution II, DNA extraction solution III, DNA extraction solution IV, positive control interior label, PCR reaction liquid, probe HBV-SP, enzyme mixed liquor containing heat resistant DNA polyase and uracil DNA glycosylase, quantitative hepatitis B virus reference material, hepatitis B virus positive control serum and hepatitis B virus negative control serum, wherein DNA extraction solution I contains 0.2-1.0% of lauryl sodium sulphate (mass/volume), 1.0-4.0% of Triton (volume/volume) and 0.2-1.0mol/L of guanidinium isothiocyanate; DNA extraction solution II contains 100-300mmol/L of 4-HEPES, 100-300mmol/L of sodium chloride with pH of 6.5+/-0.2 and 100-400 mu g/ml of magnetic beads; DNA extraction solution III contains 0.1-1.0% of Triton (volume/volume) and 100-300mmol/L of sodium chloride; DNA extraction solution IV contains mineral oil. The fluorescence quantitative PCR detection kit of hepatitis B virus of the invention can be used for detecting the HBV-DNA concentration in samples of serum, blood plasma or latex and the like.

The invention discloses a kit for extracting DNA/RNA of a virus by utilizing magnetic beads and a using method. The kit comprises the following eight components: an extracting solution I (cracking), an extracting solution II (binding), an extracting solution III (scrubbing), an extracting solution IV (scrubbing), an extracting solution V (eluting), magnetic bead suspension, a protease K working solution and a nucleic acid settling agent. The kit extraction method comprises the following steps: cracking, binding, scrubbing and eluting. The kit and the extraction method can be used for extracting the DNA/RNA of the viruses of different types of samples, the impurities such as proteins and lipids in the samples are effectively removed, the extracted nucleic acid is high in purity and complete in fragments, and the extraction process is safe and non-toxic.

The invention relates to the technical field of biological peptide preparation and discloses a method for preparing active peptide through euphausia superba powder. The method comprises the followingsteps that the degreased euphausia superba powder is evenly mixed with water to obtain a homogenate I; the pH is adjusted, acid proteinase is added for enzymolysis, and an enzymolysis solution II is obtained; the pH is adjusted, trypsin is added for enzymolysis, and an enzymolysis solution III is obtained; flavourzyme is added for enzymolysis, and an enzymolysis solution IV is obtained; after enzyme deactivation, cooling and centrifugation, a supernatant is taken, and an enzymolysis solution V is obtained; calcium hydroxide is added, after the pH is adjusted, defluorination is conducted, and an enzymolysis solution VI is obtained; dialysis is conducted through a semipermeable membrane, and a filtrate VII is obtained; after freeze-drying treatment, the dried active peptide product is obtained. According to the method, by adopting a fractional hydrolysis method, the yield of the micromolecule active peptide is increased, the enzymolysis solution is defluorinated, the euphausia superba active peptide with low fluorine content is obtained, the eating safety of the product is improved, the active peptide product is prepared through a freeze-drying method, the damage to the active peptide functional group is reduced, and the method helps to keep the function of the active peptide.

The invention relates to a gene detection kit, in particular to a helicobacter pylori (HP) type and drug-resistant mutation gene detection kit. The kit comprises PCR (polymerase chain reaction) solutions and nucleic acid membrane strips for HP type and drug-resistant mutation gene detection; the PCR solutions comprise a PCR solution I, a PCR solution II, a PCR solution III and a PCR solution IV; the PCR solutions also contain a pair of internal control primers respectively. The HP type and drug-resistant mutation gene detection kit can be used for distinguishing two types of HP in one test, and can be used for detecting 15 mutation types of 9 hot spot mutational loci related with drug fastness of 5 therapeutics, a VacA gene and a CagA gene are used for typing, and a reference basis is provided for judgment of illnesses. Mutation types of drug-resistant mutation detection are more and more comprehensive, and the condition of genotypic resistance of HP from which a patient suffers is quickly and comprehensively evaluated.

The invention relates to the technical field of biological medicines, in particular to a kit for quantum dot nucleic acid detection of urinary tract infection-causing pathogens. The kit comprises a detection membrane strip, a fluorescent detection solution and reaction solutions, wherein the detection membrane strip comprises a nylon membrane and a capture probe fixed to the nylon membrane; the fluorescent detection solution comprises quantum dots for marking the capture probe and coupled with streptavidin on the surfaces; the reaction solutions include a reaction solution I, a reaction solution II, a reaction solution III and a reaction solution IV. The kit has the beneficial effects as follows: the high-throughput, high-sensitivity and high-specificity kit for the quantum dot nucleic acid detection of the urinary tract infection-causing pathogens is provided; the kit has fewer steps and obviously shorter detection time than the existing colorimetric gene chip, has lower equipment cost than an organic fluorescent gene chip, and is conducive to clinical popularization.

The invention relates to a method for modifying activated carbon and an application of modified activated carbon and belongs to the technical field of new material preparation. The method comprises the following steps: firstly, adding the activated carbon into concentrated nitric acid, mixing, refluxing and stirring, thereby acquiring a modified product I; uniformly mixing thionyl chloride with DMF, thereby acquiring a mixed organic solution I; adding the modified product I into the mixed organic solution I, electromagnetically stirring and drying, thereby acquiring a modified product II; uniformly mixing ethanediamine with DMF, thereby acquiring a mixed organic solution II; adding the modified product II into the mixed organic solution II and electromagnetically stirring, thereby acquiring a modified product III; uniformly mixing tetrahydrofuran, triethylamine and chloroacetyl chloride, thereby acquiring a mixed organic solution III; adding the modified product III into the mixed organic solution III and stirring, thereby acquiring a modified product IV; adding the modified product IV into cuprous chloride and 4,4'-bipyridine, thereby acquiring a mixture; uniformly mixing vinyl imidazole with DMF, thereby acquiring a mixed organic solution IV; adding the mixture into the mixed organic solution IV, thereby acquiring the modified activated carbon. Compared with the untreated activated carbon, the modified activated carbon has higher adsorbing capacity.

The invention discloses a preparation method of modified lactobacillus acidophilus peptidoglycan. The preparation method is characterized by comprising the following steps of: sterilizing lactobacillus acidophilus into a water bath, adding the lactobacillus acidophilus into a TritonX-100 solution to react, and then, centrifugally washing to remove impurities in sediments to obtain a wet thallus; centrifuging after adding the wet thallus into an enzyme solution I to react, centrifuging after placing the sediments into an enzyme solution II to react, and centrifuging after adding the sediments into an enzyme solution III to react to obtain an enzymolysis wall broken thallus; washing and degreasing the enzymolysis wall broken thallus, and then, adding the enzymolysis wall broken thallus into an enzyme solution IV to remove proteins; dialyzing and salting out the degreased and protein-removed sediments, centrifuging and dialyzing the sediments, and then, freezing and drying to obtain the lactobacillus acidophilus peptidoglycan; and adding the lactobacillus acidophilus peptidoglycan into tetramentylniperidine to react with NaBr and NaClO to obtain an oxidized and modified lactobacillus acidophilus peptidoglycan product. The preparation method has the advantages of simplicity in operation, short reaction time and remarkable improvement of solubility and antioxidant activity.

The invention relates to a method for separating saturated hydrocarbons and aromatic hydrocarbons from lightweight cycle oil to mainly solve a problem of analytic result distortion of the saturated hydrocarbons and the aromatic hydrocarbons in the high-aromatic-hydrocarbon-content lightweight cycle oil in present separation technologies. The method which treats the lightweight cycle oil as an analytic material comprises the following steps: 1, adsorbing the analytic material with an adsorption column of a silica adsorbent I, flushing with an n-pentane solvent I to obtain a solution I, adsorbing through allowing the solution I to enter an adsorption column of an alumina adsorbent II, and flushing with the n-pentane solvent I to obtain a solution II; 2, flushing the adsorption column of the silica adsorbent I with a dichloromethane solvent II to obtain a solution III; 3, flushing the adsorption column II of the alumina adsorbent II with the dichloromethane solvent II to obtain a solution IV; 4, steaming the solution II to remove the n-pentane solvent I, and obtaining the content of the saturated hydrocarbons through a normalization method; and 5, steaming a mixture of the solution III and the solution IV to remove the dichloromethane solvent II, and obtaining the content of the aromatic hydrocarbons through the normalization method. The method well solves the problem through above technical scheme and can be applied to the industrial analysis of the saturated hydrocarbons and the aromatic hydrocarbons in the lightweight cycle oil.

The invention discloses a method for preparing biodiesel by using aquatic product processing scraps, which comprises the following steps: (1) boiling the cleaned fish processing scraps to between 100 and 120 DEG C, then adding an organic solvent into the scraps, and stirring and filtering the scraps to obtain solution I; (2) adding aqueous of phosphoric acid in an amount of 20 to 50 percent basedon the volume of the solution I into the solution I to obtain solution II, adding water with the same volume as the solution II into the solution II, carrying out acid washing and water washing, and dehydrating the solution II through a polyhydroxy water absorbent resin column to obtain solution III; (3) adding an alkali catalyst and anhydrous ethyl alcohol into the solution III to carry out transesterification reaction so as to obtain solution IV; and (4) standing the solution IV, removing a lower glycerine layer, then washing the generated mixed fatty acid ethyl ester layer by water, and removing the residual catalyst, the ethyl alcohol and other water soluble components to obtain the biodiesel. The method has the advantages of making full use of the prior fish processing scraps, reducing the pollution, improving the economic benefit of enterprises, and developing a new path for the utilization of aquatic product processing wastes and the development of biological energy.

The invention belongs to the technical field of the planting of dracaena sanderiana and specifically provides a rooting-sprouting nutrition solution for dracaena sanderiana. The nutrition solution is formed by mixing a mother solution I, a mother solution II, a mother solution III and a mother solution IV in a volume ratio of (7-10) to (2-3) to (2-3) to (0.05-0.1), wherein the mother solution I comprises the following substances: ammonium nitrate, monopotassium phosphate, potassium nitrate, 300mg/L-450mg/L of calcium chloride and magnesium sulfate; the mother solution II comprises the following substances: ferric sulfate, manganese sulfate, sodium molybdate, cobalt chloride and boric acid; the mother solution III comprises the following substances: tryptophan, threonine and diisopropanolamine; and the mother solution IV comprises the following substances: humic acid, diphenylurea sulfonic calcium, catechol, metalaxyl, amine acetylenic acid and Shentouxing (a wetting agent). The nutrition solution has growth-accelerating, sprouting-promoting, branch-strengthening, leaf-greening, rooting and root-strengthening effects to the dracaena sanderiana, is easy to produce, convenient to use and low in cost and further has a great significance in popularization.

A method for producing an aqueous dispersion of at least two prepolymers, each prepolymer being at least partially modified by a graft addition polymer produced in a polymer solution, the method comprising the steps of: i) providing A solution of a first prepolymer consisting of a diepoxy resin and at least one other prepolymer than polyester in an organic carrier liquid, ii) combining the polymer-containing solution with an ethylenically unsaturated mixing monomers comprising an effective amount of a copolymerizable disperse moiety, iii) providing an effective amount of a graft polymerization initiator, iv) allowing or causing the monomers to polymerize and graft to at least some of the prepolymer to form a modified A solution of the polymer, v) optionally adding a crosslinking agent to the solution iv), vi) dispersing the solution of the modified polymer and the optional crosslinking agent in an aqueous medium to form a stable dispersion of particles.

The invention discloses a thermo-sensitive CTP (cytidine triphosphate) version developing solution, which comprises the following components: deionized water, metol, anhydrous sodium sulphite, sodium metaborate, potassium thiocyanate, potassium bromide, phenidone and fluorinated alkyl. The invention also discloses a preparation method of the thermo-sensitive CTP version developing solution, which comprises the following steps: firstly, adding the anhydrous sodium sulphite into the deionized water to stir for the first time to prepare a mixing solution I; then, adding the metol into the mixing solution I to stir for the second time to prepare a mixing solution II; subsequently adding the sodium metaborate, the phenidone and the potassium bromide into the mixing solution II to stir for the third time to prepare a mixing solution III; then, adding the potassium thiocyanate into the mixing solution III to stir for the fourth time to prepare a mixing solution IV; and finally, adding the fluorinated alkyl into the mixing solution IV to stir for the fifth time to obtain the thermo-sensitive CTP version developing solution. The developing solution disclosed by the invention has the advantages of wide allowable operating temperature range, good density stability of an image-text part, great tolerance level and the like and does not damage an aluminum sheet base.

The invention discloses a soil DNA sample preparation reagent box and a manufacturing method, the reagent box comprises a box body and a baffle board, a reagent bottle A which is filled with solution I, a reagent bottle B which is filled with solution II, a reagent bottle C which is filled with solution III, a reagent bottle D which is filled with solution IV, a reagent bottle E which is filled with suspension liquid, a centrifuge tube F which is filled with acid cleaning glass bead, a mini filter G and a PCR(chain type polyase reaction) pipe H. A reagent box which is designed through the technical proposal of the invention has the advantages of simple employment and stable result, which can effectively save time and cost of users.

The invention discloses a hydroxyl nanometer magnetic ball method RNA extraction kit, and a method for fast and simply extracting sclerotium-producing fungus RNA. The kit comprises a solution I, a solution II, a solution III, a solution IV, a washing solution, an eluant and a hydroxyl nanometer magnetic ball suspension. The solution II is creatively used for removing polysaccharide substances of sclerotium-producing fungus, so that in the RNA extraction process, the interference of exopolysaccharides is avoided. In the experiment process, the hydroxyl nanometer magnetic balls used by the kit are also creatively pretreated; the RNA is used as a molecular target; the adsorption capability of the hydroxyl nanometer magnetic balls on DNA and proteins is reduced; the operation has the obvious characteristic of high specificity. The kit is applicable to RNA extraction of the sclerotium-producing fungus; the detection is simple, convenient and fast; the interference of the exopolysaccharideson the RNA extraction is reduced.

The invention discloses an eczema ointment and a preparation method thereof. The method comprises the following steps that 1) periostracum cicada, astragalus membranaceus, angelica sinensis, frankincense, myrrh, realgar and golden larch bark are smashed and immersed, and heat-under-reflux is carried out; filtering is carried out, a solvent is evaporated to obtain a Chinese herbal extract I; 2) decoction is conducted on wormwood and chrysanthemum indicum, and concentration is carried out to obtain a water extract II; 3) the Chinese herbal extract I, the water extract II, lotus seed powder and honey are mixed to prepare a mixed medicament III; 4) glycerin monostearate and aloe vera gel are mixed to be conducted with ultrasonication and heating to obtain a solution IV; 5) tacrolimus and a dispersing agent are mixed with a mixed liquid, and the solvent is evaporated to obtain a crude eczema ointment; 6) after high pressure homogenization is conducted on the crude material, the eczema ointment is obtained. The prepared eczema ointment has the functions of clearing damp, relieving itching, regulating immunity, preventing inflammatory and itch, clearing heart fire, tranquilizing mind by nourishing the heart, activating blood circulation, regulating qi, relieving pain, eliminating swelling, eliminating stagnation, promoting tissue regeneration, turning fester caused by pruritus and empyesis into tissue regeneration, and effecting a radical cure without any side effects.

The invention relates to a preparation method and an application of a molybdenum and nitrogen doped lignocellulose compound adsorbing nano-material. The method includes the steps: (1) calcining lignocellulose in the ammonia atmosphere, naturally cooling the calcined lignocellulose, and grinding the cooled lignocellulose to obtain nitrogen doped lignocellulose; (2) dissolving ammonium molybdate indeionized water to prepare solution I; (3) dissolving thioacetamide in deionized water to prepare solution II; (4) sufficiently mixing the solution I and the solution II to prepare solution III; (5) adding the nitrogen doped lignocellulose into the solution III, and stirring mixture to obtain solution IV; (6) transferring the solution IV into a high-pressure reaction kettle, and performing high-temperature reaction; (7) cooling the solution IV of high-pressure reaction, and centrifugally separating a reaction product to obtain the molybdenum and nitrogen doped lignocellulose compound adsorbingnano-material. According to the method, a molybdenum and nitrogen doped lignocellulose compound has good adsorption effects on hexavalent chromium, the adsorption performance of the compound basically keeps invariable, and the compound has good stability.

The invention relates to the field of food chemistry, in particular to mixed gel and a preparation method thereof. The mixed gel mainly comprises, by mass, 2.5-4% of agar, 2.5%-4% of carrageenan, 30%-40% of gelatin, 9%-12% of glycerin and the balance water. The preparation method includes steps: adding agar, carrageenan and glycerin, and swelling to obtain mixed solution I; adding gelatin into water, and swelling to obtain solution II; slightly cooling the solution I and the solution II, and performing equal-mass mixing to obtain colloidal solution III; heating the solution III to form stablecolloidal mixed solution IV; pouring the solution IV into a spherical mould, and condensing naturally to obtain agar, carrageenan and gelatin mixed gel. The mixed gel has characteristics of high elasticity and less proneness to breakage while the water binding capacity is greatly increased, transparency of the gel is improved, and the water binding capacity is further increased.

The invention discloses a synthesis method of a slump-retaining polycarboxylic acid high-performance water-reducing agent. The synthesis method comprises the following synthesis steps: firstly, addingdeionized water and methallyl alcohol polyoxyethylene ether into a reaction still, stirring and heating to 60+/-2 DEG C; secondly, preparing a solution I: adding the deionized water, ascorbic acid and thiohydracrylic acid; preparing a solution II: adding the deionized water and acrylic acid; thirdly, preparing a solution III: adding the deionized water, the ascorbic acid and the thiohydracrylic acid; preparing a solution IV: adding the deionized water and the acrylic acid; fourthly, adding hydrogen peroxide into the first step, uniformly dropwise adding the solution I and the solution II prepared in the second step respectively; then dropwise adding the solution III and the solution IV prepared in the third step; fifthly, carrying out aging reaction for 60 minutes; sixthly, reducing the temperature to below 45 DEG C, adding caustic soda liquid containing 30 percent of sodium hydroxide and neutralizing until a pH value is 6 or so. The synthesis method has the beneficial effects that production cycle is shortened from original 5 to 6 hours to 3 hours now and the qualification rate of a product is increased from original 80 to 90 percent to 100 percent now.

The invention discloses a surface modified cyano frame material and a preparation method and application thereof. The method comprises the steps that sodium ferrocyanide and sodium pentacyanoferrate are mixed with deionized water to acquire a solution I; soluble divalent manganese salt and soluble divalent acetate are mixed with deionized water to acquire a solution II; sodium salt is mixed with deionized water to acquire a solution III; the solutions I and II are simultaneously dropped into the solution III, and co-precipitation reaction is carried out to acquire a suspension liquid containing the cyano frame material; sodium hydroxide is mixed with deionized water to acquire a solution IV; the solution IV is added to the suspension liquid dropwise, and thoroughly stirred and post-treatedat a certain temperature to acquire a surface-modified cyano frame material. According to the invention, through simultaneous doping and surface coating, the structural stability of the cyano frame material is effectively improved, and corrosion of an electrolyte on a product is inhibited.

The invention discloses a preparation method for a porphyrin-functionalized titanium dioxide-montmorillonite nano-complex. The preparation method comprises the following steps: adding tetrabutyl orthotitanate into absolute ethyl alcohol, and stirring to obtain a transparent solution I; slowly adding the transparent solution I into nitric acid solution dropwise to obtain a solution II; adding montmorillonite into deionized water to prepare montmorillonite suspension III; slowly adding the solution II into the montmorillonite suspension III, and stirring to obtain a white solution IV; quickly pouring the white solution IV into a reaction kettle, and placing reaction kettle into a microwave hydrothermal reaction kettle for reacting; after the reaction is ended, naturally cooling to room temperature, then, filtering, performing centrifugal washing, and drying to obtain a product A; dispersing the product A into the deionized water; then, performing mixed reaction with a tetraphenylporphyrin solution; and after the reaction is ended, performing centrifugal treatment, washing and drying to obtain a final product. The nano-complex prepared by adopting the process disclosed by the invention has the advantages that on one hand, the catalytic efficiency of the nano-complex is improved, and on the other hand, the optical response range of the nano-complex is expanded.

The invention discloses an efficient foaming type cellulose hydrogel fire extinguishing agent, and belongs to the technical field of fire extinguishing agent production. The fire extinguishing agent is prepared from the following raw material components: A, a cellulose material, B, bicarbonate, C, soluble aluminum salt, and D, water. The preparation method comprises the following steps of completely dissolving 1 part of the component A in 50-200 parts of water to obtain a solution I, wherein the mass fraction of the component A in the solution I is 0.5-2 wt%; dissolving 1 part of the componentB in 100-200 parts of water to obtain a solution II, wherein the mass fraction of the component B in the solution II is 0.5-1wt%; mixing the solution I and the solution II in a closed space to obtaina solution III; dissolving one part of the component C in 100-200 parts of water to obtain a solution IV, wherein the mass fraction of D in the solution IV in water is 0.5-1 wt%; and spraying the mixed solution III and the mixed solution IV to the surface of the comburent at the same time or spraying the mixed solution III to the surface of the comburent firstly, and then spraying the mixed solution IV to the surface of the comburent.