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Method for preparing active peptide through euphausia superba powder

A technology of Antarctic krill powder and active peptides, which is applied in the field of active peptide preparation, can solve the problems of low yield of small molecule active peptides, achieve the effect of maintaining the function of active peptides, increasing the yield, and reducing damage

Active Publication Date: 2019-06-28
OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of small molecule active peptides obtained by this method is low

Method used

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  • Method for preparing active peptide through euphausia superba powder

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Embodiment 1

[0036] A method for preparing active peptides using Antarctic krill powder, comprising the following steps:

[0037] (1) defatted Antarctic krill powder and water are mixed in a ratio of 1:3 to obtain homogenate liquid I;

[0038] (2) Use 2M phosphoric acid solution to adjust the pH of homogenate I to 3.2, add pepsin after preheating to 42°C, the concentration of pepsin in homogenate I is 600U / g, and enzymolyze it at 42°C for 3 hours Liquid II;

[0039] (3) Use 2M sodium hydroxide solution to adjust the pH of the enzymolysis solution II to 7.2, add trypsin, the concentration of trypsin in the enzymolysis solution II is 50U / g, and obtain the enzymolysis solution after enzymolysis at 47°C for 3 hours III;

[0040] (4) Add flavor protease to enzymolysis solution III, the concentration of flavor protease in enzymolysis solution III is 150U / g, and enzymolysis solution IV is obtained after enzymolysis at 47° C. for 1 hour;

[0041] (5) Inactivate the enzyme solution IV at 92°C for ...

Embodiment 2

[0046] A method for preparing active peptides using Antarctic krill powder, comprising the following steps:

[0047] (1) The defatted Antarctic krill powder and water are mixed with a mass ratio of 1:2.5 to obtain homogenate I;

[0048] (2) Use a 2.5M phosphoric acid solution to adjust the pH of homogenate I to 3.5, add papain after preheating to 45°C, the concentration of papain in homogenate I is 500U / g, and obtain after enzymatic hydrolysis at 45°C for 2.5h Enzyme solution II;

[0049] (3) Use a 2.5M sodium hydroxide solution to adjust the pH of the enzymolysis solution II to 7.5, add trypsin, the concentration of trypsin in the enzymolysis solution II is 80U / g, and obtain the enzyme after enzymolysis at 50°C for 2.5h Solution III;

[0050] (4) Add flavor protease to enzymolysis solution III, the concentration of flavor protease in enzymolysis solution III is 220U / g, enzymolysis at 50°C for 1.5h to obtain enzymolysis solution IV;

[0051] (5) Inactivate the enzyme soluti...

Embodiment 3

[0056] A method for preparing active peptides using Antarctic krill powder, comprising the following steps:

[0057] (1) defatted Antarctic krill powder and water are mixed in a ratio of 1:2 to obtain homogenate liquid I;

[0058] (2) Adjust the pH of the homogenate I to 3.0 with a 1.5M phosphoric acid solution, add pepsin after preheating to 40°C, the concentration of pepsin in the homogenate I is 700U / g, and obtain the enzyme after enzymatic hydrolysis at 40°C for 2 hours Solution II;

[0059] (3) Use a 1.5M sodium hydroxide solution to adjust the pH of the enzymolysis solution II to 7.0, add trypsin, the concentration of trypsin in the enzymolysis solution II is 80U / g, and enzymolyze at 45°C for 2 hours to obtain enzymolysis Liquid III;

[0060] (4) Add flavor protease to enzymolysis solution III, the concentration of flavor protease in enzymolysis solution III is 220U / g, enzymolysis at 45°C for 2 hours to obtain enzymolysis solution IV;

[0061] (5) Inactivate the enzym...

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Abstract

The invention relates to the technical field of biological peptide preparation and discloses a method for preparing active peptide through euphausia superba powder. The method comprises the followingsteps that the degreased euphausia superba powder is evenly mixed with water to obtain a homogenate I; the pH is adjusted, acid proteinase is added for enzymolysis, and an enzymolysis solution II is obtained; the pH is adjusted, trypsin is added for enzymolysis, and an enzymolysis solution III is obtained; flavourzyme is added for enzymolysis, and an enzymolysis solution IV is obtained; after enzyme deactivation, cooling and centrifugation, a supernatant is taken, and an enzymolysis solution V is obtained; calcium hydroxide is added, after the pH is adjusted, defluorination is conducted, and an enzymolysis solution VI is obtained; dialysis is conducted through a semipermeable membrane, and a filtrate VII is obtained; after freeze-drying treatment, the dried active peptide product is obtained. According to the method, by adopting a fractional hydrolysis method, the yield of the micromolecule active peptide is increased, the enzymolysis solution is defluorinated, the euphausia superba active peptide with low fluorine content is obtained, the eating safety of the product is improved, the active peptide product is prepared through a freeze-drying method, the damage to the active peptide functional group is reduced, and the method helps to keep the function of the active peptide.

Description

technical field [0001] The invention relates to the technical field of preparation of active peptides, in particular to a method for preparing active peptides using Antarctic krill powder. Background technique [0002] Active peptide is the most important active substance in the human body, which plays a key role in human growth and development, metabolism, disease, aging and death. It is precisely because of the increase or decrease of its secretion in the body that human beings have a cycle of infancy, childhood, adulthood, old age and death. There are two main extraction methods for peptides, one is chemical extraction and the other is physical extraction. "Chemical extraction method" is mainly "chemical enzymatic reaction extraction method", which has fast reaction and large production capacity, but the disadvantage is that the activity of the extracted peptides is low. This method is the main source of peptide products on the market; "physical extraction "Method" main...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30
CPCY02P20/10
Inventor 肖金星郑刚郑凯杨志坚周宇芳相兴伟泮红文
Owner OCEAN RES CENT OF ZHOUSHAN ZHEJIANG UNIV
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