193results about How to "Preserve activity" patented technology

This invention relates to compounds, compositions, and methods useful for modulating Connexin gene expression using short interfering nucleic acid (siNA) molecules. This invention also relates to compounds, compositions, and methods useful for modulating the expression and activity of other genes involved in pathways of Connexin gene expression and / or activity by RNA interference (RNAi) using small nucleic acid molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siNA), double-stranded RNA (dsRNA), micro-RNA (mRNA), and short hairpin RNA (shRNA) molecules and methods used to modulate the expression of Connexin genes such as Connexin43 (Cx43).

The present invention concerns methods and reagents useful in modulating MAP kinase gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating RNA interference (RNAi) against c-JUN, JNK, p38, and ERK gene expression, useful in the treatment of cancer, inflammation, obesity and insulin resistance (e.g. Type I and Type II diabetes).

The present invention concerns methods and reagents useful in modulating gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications associated with Alzheimer's disease. Specifically, the invention relates to small interfering RNA (siRNA) molecules capable of mediating RNA interference (RNAi) against NOGO and NOGO receptor (NOGOr) polypeptide and polynucleotide targets.

The invention discloses a multienzyme organic fertilizer, wherein the fertilizer can increase soil organic matter contents and varieties of bioactivators, and improve physical, chemical and biological characteristics for soil, and improve soil fertility, and alleviate soil hardened state, and reduce pesticide residue in the soil; on the other hand, the fertilizer can provide progressive, continuous and complete nutrients for plant growth, and improve inner environment of crops at the same time such that the crops have abilities of full digestion and nutrient absorption. The invention can not only improve the yields of the crops, but also can improve qualities of agricultural products, and enhance healthcare functions for agricultural products. The fertilizer is suitable to be used by field crops and greenhouse vegetables, land vegetables, fruits, flowers, commercial crops and so on. The multienzyme organic fertilizer is composed of the following raw materials in percentages by weight: 10-15% of laccase, 5-10% of neutral protease, 10-15% of phytase, 5-10% of cellulose, 1-5% of xylanase, 1-5% of pectinase, 1-5% of amylase and 55-60% of organic fertilizer powder.

This invention relates to compounds, compositions, and methods useful for modulating cholesteryl ester transfer protein (CETP) gene expression using short interfering nucleic acid (siNA) molecules. This invention also relates to compounds, compositions, and methods useful for modulating the expression and activity of other genes involved in pathways of CETP gene expression and / or activity by RNA interference (RNAi) using small nucleic acid molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules and methods used to modulate the expression of CETP genes.

The present invention relates to a dissolvent with effective ingredient extracted from the plant-and-animal raw material and the extraction method thereof. One subcritical fluid extraction solvent includes an extraction solvent of a compound or composition extracted from the raw material containing compound or composition as component element, and the solvent is mainly characterized in that the liquid sulfur hexafluoride works as the extraction solvent. The invention also includes a method for extracting the subcritical fluid extraction solvent. The advantage of the invention is that the extraction and separation of the target component in the natural product are optionally executed to further increase the extraction rate of the intermediate product and the usage factor of the raw material while the unique dissolving character of the sulfur hexafluoride and the mixed solvent thereof to the liposoluble active ingredient is used. The physical features of strong dissolving capacity and low boiling point of the sulfur hexafluoride and the mixed solvent thereof are used to reduce the consumption quantity of the energy source, dissolvent of the system and the cost, and increase the market competition ability of the intermediate product.

The invention relates to a method for preparing thrombin-chitosan self-assembly nano particles and application thereof. The method comprises: adding chitosan into an acetic acid aqueous solution to form a chitosan solution; adding thrombin into a buffer solution to form a thrombin solution; uniformly mixing the thrombin solution and the chitosan solution, adding a crosslinking agent for stirring, pouring the mixture into liquid paraffin for stirring, adding a dispersant, and performing emulsification; and adding the crosslinking agent for crosslinking reaction through water bath, performing filtration and acetone washing, using ethanol for washing and dehydration, and finally performing vacuum drying to obtain the thrombin-chitosan self-assembly nano particles. The method feeds the thrombin and the chitosan according to proportion to prepare thrombin-chitosan which is self-assembled into nano particles for the first time, maintains the stability of the thrombin of the thrombin-chitosan self-assembly nano particles, and gives better play to the comprehensive hemostatic effect of the thrombin and the chitosan of the thrombin-chitosan self-assembly nano particles. The method can be widely applied to tissue hemostasis, tissue sealing and tissue adhesion in the surgical fields such as general surgery, orthopaedics, heat and chest surgery, neurosurgery and gynaecology and obstetrics.

An instant refined wolfberry fruit powder is prepared from wolfberry fruit, milk, vegetable butter powder and additive through screening, washing, immersing, extracting, solid-liquid separation, squeezing the solid to obtain juice, deposition, filtering proportionally mixing and drying. Its advantage is easy absorption of active components.

The invention relates to a curcumin or curcumin-ramification-containing composite feed additive and a use thereof. The use of the additive can promote the growth of the animals, and / or protect the livers of the animals, and / or improve the oxidation resistance of the animals, reduce the generation of lipid peroxide, and improve the immunity and the disease resistance, and / or improve the stress resistance of the fish, avoid the problem of 'irritable haemorrhage', promote the secretion of fish mucus, and solve the problems that the cultivated fish is easy to loosen squama, easy to fall off, and lack of fish mucus. The invention further relates to a method for cultivating the animals and an animal feed which contains the composite feed additive having the curcumin or curcumin ramification.

The invention discloses a preparation method of a novel dispersant, which comprises the steps of: taking crylic acid as a main reaction monomer; adding at least one of monomers such as methacrylic acid, maleic anhydride, fumaric acid, itaconic acid and methyl sodium allylsulfonate as comonomer; taking r-methacryloxypropyl trimethoxy silane, vinyl trimethoxy silane or vinyl triethoxysilane as organic silicon monomer to participate in polymerization; taking a redox system as an initiating system; and polymerizing by water solution, and neutralizing by alkaline substance to obtain the novel dispersant. Due to the novel dispersant, the grinding of calcium carbonate has the characteristics of being low in sizing agent viscosity, small in grain size, good in dispersibility and high in stability.

The present invention provides a lycium ruthenicum murr. vinegar fermentation method, and two lycium ruthenicum murr. vinegar drink preparation methods. The lycium ruthenicum murr. vinegar takes fresh lycium ruthenicum murr. as the raw material, and is obtained by the selection of raw materials, juicing, low-temperature alcoholic fermentation, acetic acid fermentation, ageing, filtration, sterilization and other process procedures. The resulting lycium ruthenicum murr. vinegar is blended to obtain a lycium ruthenicum murr. vinegar drink. Anthocyanin, organic acids, vitamins, VC and other active ingredients, especially anthocyanin contained in the lycium ruthenicum murr. are maximally preserved by the high acid environment of the lycium ruthenicum murr. vinegar and the vinegar drinks. Therefore, the lycium ruthenicum murr. vinegar is superior to table vinegar taking grain as raw materials, has a sweet and sour taste and pure flavor, is sour, cool and soft, nutrient-rich and high-quality, and has the efficiency of invigorating stomach, promoting digestion, tonifying, protecting health and the like.

The invention relates to a plant drying and essential oil and hydrolat extraction system based on air energy.The plant drying and essential oil and hydrolat extraction system comprises a drying oven, a condenser, an oil-water separator and an air energy heating and cooling unit.The drying oven is used for drying plants and volatilizing moisture and essential oil in the plants to form oil-water gaseous state mixture.The condenser is connected with the drying oven and used for lowering the temperature of the gaseous state mixture and converting the gaseous state mixture into liquid state mixture.The oil-water separator is used for separating the liquid state mixture into essential oil and hydrolat.The air energy heating and cooling unit heats the drying oven and cools the condenser.Compared with the prior art, the system improves device production efficiency and greatly lowers device energy consumption; due to high system integration, the device site requirements are lowered, the device structure is simplified, the device investment is lowered, the device operation difficulty and maintenance cost are lowered, and environmental pollution caused by exhaust emission is avoided.

Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into / out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window, a heat exchanger, inlet / outlet flow arrangements, an inspection window, insert elements that define a treatment volume size or shape, etc. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

The invention belongs to the technical field of plant protein drinks and in particular relates to a walnut protein drink and a preparation method thereof. The walnut protein drink provided by the invention is composed of water, walnut kernels, a food stabilizer, sodium bicarbonate, xanthan gum, sodium erythorbate, nisin and cane sugar. The preparation method comprises the following specific steps of: removing walnut skins on the surfaces of the walnut kernels; preparing the walnut kernels into pulp and pumping into a mixing cylinder; adding the food stabilizer, sodium bicarbonate, the xanthan gum, sodium erythorbate, the nisin, the cane sugar and hot water into a sugar melting pot; uniformly mixing and pumping into the mixing cylinder; and finally, adding a certain amount of water into the mixing cylinder, and sterilizing and packaging after adjusting the pH value. The walnut protein drink provided by the invention has high plant protein content, uniform material juice and good mouth feel and does not contain suspended matters.

The present invention concerns methods and reagents useful in modulating adenosine A1 receptor (ADORA1) gene expression in a variety of applications, including use in therapeutic, diagnostic, target validation, and genomic discovery applications. Specifically, the invention relates to small interfering RNA (siRNA) molecules capable of mediating RNA interference (RNAi) against ADORA1 and related receptors.

The invention relates to the field of biology, and in particular relates to a saliva protection agent as well as a preparation method and application thereof. Each 1000ml of the saliva protection agent consists of 10-50mmol of tris-hydroxymethyl aminomethane, 0.5-20mmol of ethylenediaminetetraacetic acid and / or sodium ethylene diamine tetracetate, 200-1000mmol of ammonium sulfate and 1-50mmol of glucose. Saliva, preserved in the saliva protection agent disclosed by the invention, is nearly free from change in the first 60 days, still complete in DNA and free from obvious degradation; the saliva is remained so after 6 months; and after 12 months, the saliva still remains the integrity of DNA as before. The saliva protection agent disclosed by the invention can be used for quite effectively preserving a saliva sample and for guaranteeing the integrity and long-term stability of the sample.

The invention discloses a low-temperature refining method of tea seed oil, comprising an absorbing and refining step and a filtering step. The invention adopts the core technology of utilizing a mixed absorption agent to absorb and filter impurities in crude oil, such as free fatty acid, various pigments, phospholipid, and the like to achieve the aim of refining the tea seed oil so as to substitute traditional procedures, such as degumming, deacidifying, bleaching, deodorizing, and the like. The invention adopting a low-temperature physical absorption technology only needs an absorbing and refining pot and filtering equipment, reduces the traditional refining procedures, shortens the production period, lessens the refining loss of the tea seed oil, avoids the execution of the high-temperature process of the traditional refining process, reserves biological active substances and nutrient contents in the tea seed oil, such as vitamins, squalene, and the like and improves the product quality and the health function. The tea seed oil obtained through refining is colorless and transparent and has high yield, equipment investment is small, and the method is simple and suitable for industrial production, keeps the pure natural characteristics of the tea seed oil and greatly improves the added values of the tea seed oil.

Methods and systems for acoustically treating material using a continuous process in which material may be caused to flow in a continuous or intermittent fashion into / out of an acoustic treatment chamber where the material is exposed to focused acoustic energy. The methods and systems may be arranged to permit continuous processing for extended periods while an acoustic energy source operates at a relatively high power output. Treatment chambers may include features such as an acoustic window, a heat exchanger, inlet / outlet flow arrangements, an inspection window, insert elements that define a treatment volume size or shape, etc. Treatment system configurations relating to arrangements of a treatment chamber relative to an acoustic source and coupling medium, material flow paths, and others are provided.

The invention discloses a method which adopts the surface of a titanium and titanium alloy cardiovascular implantation device to fix CD34 antibody or CD133 antibody directionally, and comprises the following main steps: A. activation treatment is carried out, so as to form hydroxyl on the surface; B. a layer of avidin is adsorbed on the activation surface of the step A; C. a layer of biotinylated protein A is adsorbed by the avidin layer; D. on the surface of the protein A layer obtained, a layer of specific antibodies of the membrane protein of endothelial progenitor cells- CD34 antibodies or CD133 antibodies are fixed directionally, so the titanium and titanium alloy cardiovascular implantation device can capture the endothelial progenitor cells in blood after being implanted into the human body, thereby leading to rapid endothelialization of the implantation device surface and leading the implantation device to have excellent anticoagulant function and restenosis occurring resistance function.

The invention discloses a microcapsule peruvian ginseng tea and a preparation method thereof. The microcapsule peruvian ginseng tea comprises the following components in parts by weight: 10-50 parts of peruvian ginseng raw powder, 0.52-2.61 parts of a microcapsule wall material, 1-20 parts of a reorganizing pelleting agent, and 10-100 parts of tea leaves or a tea replacement beverage. The preparation method of the microcapsule peruvian ginseng tea comprises the following steps: S1, microencapsulated embedding; S2, pelleting composite slow-release particles; and S3, compounding peruvian ginseng composite slow-release particles, the tea leaves or the tea replacement beverage, and mixing the peruvian ginseng composite slow-release particles, the tea leaves or the tea replacement beverage so as to obtain finished products. The microcapsule peruvian ginseng tea prepared by the preparation method completely preserves the overhead price nutrition and the active components of the peruvian ginseng raw powder, does not contain any chemical preservatives or pigment, and is a natural green health-care beverage.

The present invention pertains to a method for producing a medicinal product comprising a biologically active protein comprising the steps of providing an aqueous composition comprising a solvent, the biologically active protein and between 20% w / w and 60% w / w of a non-polymeric sugar, freezing the composition, thereby forming at least one frozen body comprising the solvent in frozen form, putting the frozen body in a drying apparatus while being carried by a support, the support comprising one or more restraining elements that define one or more boundaries of the support, wherein at most 30% of the surface of the body is contiguous with the one or more restraining elements, reducing the pressure in the drying apparatus below atmospheric pressure, providing heat to the body in order to sublimate the frozen solvent of the body and obtain a dried body. The invention also pertains to a product obtainable by this method.

The invention provides high-pressure homogeneous honey and a preparation method for a product thereof. Specifically, the method comprises the following steps: crushing and filtering pure honey or royal jelly, pollen and bee pupa respectively, taking filter liquor of any one or a filter liquor mixture of any two or any three of the materials to be mixed with 80 to 95 percent honey after vacuum concentration at the temperature of between 10 DEG C below zero and 30 DEG C, decompressing and stirring the honey or the mixture repeatedly, and delivering the honey or the mixture into a high-pressure homogenizer of 70MPa to be compressed and homogenized to be even. The royal jelly, the pollen, the bee pupa and the honey are stirred in vacuum and then are compressed and homogenized by adopting the high-pressure homogenizer of 70MPa to form a stable combination so as to be prepared into nutrient honey. The method adopts the high-pressure homogenizer of 70MPa and uses different parameter indexes to achieve the aims of improving the grain diameter and granularity size of the honey, improving the refined mouthfeel of the honey, enhancing the absorption of the human body to nutrient substances, realizing the cooperative application of the high-pressure homogenizing technology and the prior ultra-filtration and purification technology, and greatly improving the quality of the honey.

A process for preparing the active embryonic peptide with coproduct of corn oil from the embryo bud of corn at the same time includes such steps as wet and thermal pretreating, grinding by colloid mill, multi-stage zymolysis, centrifugal separation to obtain fibres, corn oil and milk, concentrating the milk by nanometre filtering membrane, further concentrating, homogenizing, emulsifying, drying and granulating.

The invention discloses a preparation method of a 3D tissue engineering cell ring. By utilizing a relatively narrow growth space and a surface non-adhesion characteristic of a culture mold, added cells are self-assembled into a ring in a culture solution for promoting cell aggregation and leave the surface of a cell culture medium, and a macroscopic tissue block or organoid with a three-dimensional structure is formed. A naturally-formed extracellular matrix exists between the cells prepared by the method, the exogenous scaffold interference does not exist, and the communication and the information exchange between the cells are more complete and direct so as to reduce the difference between a culture environment and an in-vivo environment and more truly simulate an in-vivo cell growth environment. Moreover, the prepared cell ring is a cell body with a regular annular structure, can simulate the in-vivo cell microenvironment, links a 2D cell culture system with the research of tissuesand organs, and is beneficial to research of organoids.

The present invention mainly relates to a microwave drier, especially a microwave vacuum drier. A safe and energy-saving microwave vacuum continuous automatic drier includes a tank (A), a hoisting system (8), a vacuum system (9), a microwave heating system (1) on the tank (A), characterized by including a delivery device (6) inside the tank (A), a discharging device (5) below the delivery device (6), the materials in the tank (A) are non-metallic materials. On the basis of ensuring the strength and the toughness, the striking a light and discharging are avoided in the vacuum state when metal is heated in the microwave. The invention adopts the way of microwave radiation heating, utilizing the characters of microwave high efficiency, easy to control, antisepsis, sterilization, fast heating, even heating, fast dehydration, preserving to reduce the power waster and advance the efficiency.

The invention relates to the technical field of stem cells and especially relates to a placenta mesenchymal stem cell injection, a preparation method and application thereof. The placenta mesenchymal stem cell injection comprises placenta mesenchymal stem cells, vitamin C, dimethyl sulfoxide (DMSO), fetal calf serum (FBS), human albumin, rhizoma panacis majoris saponin and solvent. The placenta mesenchymal stem cell injection prepared according to the invention is characterized in that the materials are conveniently taken and the ethical violation problem is avoided; the injection prepared according to the invention can effectively preserve the activity of stem cells, can be directly used for recovery, need not be cleaned and centrifuged and is conveniently used; after the injection prepared according to the invention is fed back, the differentiation of the mesenchymal stem cells to the hepatic cells and the maturation of the hepatic cells can be boosted and the GVHD occurrence rate can be reduced.

The invention relates to the field of cytokine germination, in particular to preparation of umbilical cord mesenchymal stem cell factors with hair growth effect. The preparation includes the followingsteps: primary isolation and culture of human umbilical cord mesenchymal stem cells, collection of a cell culture supernatant, centrifugation, ultrafiltration and concentration to obtain a liquid containing the umbilical cord mesenchymal stem cell factor complex, and a freeze-drying process to prepare umbilical cord mesenchymal stem cell factor lyophilized powder. By the preparation method of theumbilical cord mesenchymal stem cell factors, activity of the cell factors can be preserved to the maximum and the cell factors are convenient to store and use. Hair follicle cells are fundamentallyactivated, and the hair regeneration effect is promoted remarkably and is more durable.

The invention discloses health-care noodles containing canna edulis ker and a processing method thereof. The health-care noodles containing the canna edulis ker, provided by the invention, are prepared by adding 5-50 parts of canna edulis ker starch and 0.1-1 part of alkaline powder into every 100 parts of flour. The processing method comprises the following steps: mixing the canna edulis ker starch, the flour and the alkaline powder according to the proportion of the formula, and adding clean water, wherein the proportion of the clean water to mixed powder is 5:2; putting the mixture into a dough mixer to be blended evenly, mixing the mixture into a dough, curing the dough for 30 minutes at the environment of 25 DEG C and the humidity of 30%, sending the dough to a noodle press to be pressed into a flat cake, and cutting the flat cake into strips to make wet noodles; and drying, cutting off and packaging the wet noodles to obtain the health-care noodles containing the canna edulis ker. The processing method comprises the steps of mixing ingredients, mixing flour, curing, tabletting, extending, cutting into strips, drying, cutting off and packaging. Compared with general noodles, the health-care noodles containing the canna edulis ker, disclosed by the invention, are better in mouth feel, are richer in nutrition, are good in product quality, are stable in quality, and are better pleased and welcomed by consumers; at the same time, the health-care noodles containing the canna edulis ker, disclosed by the invention, have the characteristics of simple processing technology and low production cost.