CD34 antibody or CD133 antibody surface orientation fixing method of titanium and titanium alloy cardiovascular implantation device

An implanted device and directional fixation technology, applied in prosthesis, medical science, etc., can solve problems such as thrombus formation and social loss, and achieve the effect of improving efficiency and beneficial anticoagulant function

Inactive Publication Date: 2009-02-04
深圳市金瑞凯利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although drug-coated stents can reduce the incidence of restenosis, the drug in the coating inhibits the proliferation of endothelial cells while inhibiting the proliferation of smooth muscle cells, leaving the stent exposed, leading to inflammation, coagulation and even thrombosis
[0004] At present, there are about 200,000 patients in our country who need artificial heart valve replacement surgery every year due to serious valvular disease. Most of these patients are young and middle-aged. If the valve cannot be replaced in time, it will cause immeasurable losses to the society.

Method used

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  • CD34 antibody or CD133 antibody surface orientation fixing method of titanium and titanium alloy cardiovascular implantation device
  • CD34 antibody or CD133 antibody surface orientation fixing method of titanium and titanium alloy cardiovascular implantation device
  • CD34 antibody or CD133 antibody surface orientation fixing method of titanium and titanium alloy cardiovascular implantation device

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Experimental program
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Effect test

Embodiment 1

[0035] 1. A method for directional immobilization of CD34 antibody on the surface of a titanium alloy cardiovascular implant device, the steps are as follows:

[0036] A. Activation treatment

[0037] The titanium alloy cardiovascular implant device was immersed in 0.5mol / L NaOH solution at 40°C for 1 hour, and then placed in deionized water at 60°C for 1 hour, then dried in air at 30°C; irradiated with ultraviolet light 1 minute.

[0038] B. Fixation of avidin:

[0039] Immerse the implanted device after A-step activation treatment in normal saline with avidin concentration of 0.1 mg / ml for 1 hour, then rinse with normal saline and blow dry with nitrogen after taking it out;

[0040] C. Fixation of biotinylated protein A:

[0041] Immerse the implanted device treated in step B in 0.1% albumin solution, incubate at room temperature for 5 minutes, then immerse it in physiological saline containing 0.01mg / ml of biotinylated protein A for 1 hour, and then take it out Rinse, dry with ...

Embodiment 2

[0045] 1. A method for directional immobilization of CD34 antibody on the surface of a titanium cardiovascular implant device, the steps are as follows:

[0046] A. Activation treatment

[0047] The titanium cardiovascular implant device was immersed in a 5mol / L NaOH solution at 80°C for 24 hours. After being taken out, it was placed in 90°C deionized water for 24 hours, then dried in 90°C air, and then irradiated with ultraviolet light for 30 minutes.

[0048] B. Fixation of avidin:

[0049] Immerse the implanted device after A-step activation treatment in phosphate buffer with avidin concentration of 5.0 mg / ml for 12 hours, rinse with phosphate buffer after removal, and blow dry with nitrogen;

[0050] C. Fixation of biotinylated protein A:

[0051] Immerse the implanted device treated in step B in a 5% albumin solution, incubate at room temperature for 40 minutes, and then immerse it in a phosphate buffer containing 5mg / ml of biotinylated protein A for 12 hours, and then remove...

Embodiment 3

[0055] 1. A method for directional immobilization of CD133 antibody on the surface of a titanium alloy cardiovascular implant device, the steps are as follows:

[0056] A. Activation treatment

[0057] The titanium alloy cardiovascular implant device was soaked in 3mol / L NaOH solution at 70°C for 12 hours. After being taken out, it was kept in deionized water at 70°C for 20 hours, then dried in the air at 80°C and irradiated with ultraviolet light for 20 hours. minute.

[0058] B. Fixation of avidin:

[0059] Immerse the implanted device after A-step activation treatment in a phosphate buffer solution with an avidin concentration of 1.0 mg / ml for 6 hours, take it out and rinse with phosphate buffer solution and blow dry with nitrogen;

[0060] C. Fixation of biotinylated protein A:

[0061] Immerse the implanted device treated in step B in a 1% albumin solution, incubate at room temperature for 40 minutes, and then immerse it in a phosphate buffer containing 0.1 mg / ml of biotinyla...

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Abstract

The invention discloses a method which adopts the surface of a titanium and titanium alloy cardiovascular implantation device to fix CD34 antibody or CD133 antibody directionally, and comprises the following main steps: A. activation treatment is carried out, so as to form hydroxyl on the surface; B. a layer of avidin is adsorbed on the activation surface of the step A; C. a layer of biotinylated protein A is adsorbed by the avidin layer; D. on the surface of the protein A layer obtained, a layer of specific antibodies of the membrane protein of endothelial progenitor cells- CD34 antibodies or CD133 antibodies are fixed directionally, so the titanium and titanium alloy cardiovascular implantation device can capture the endothelial progenitor cells in blood after being implanted into the human body, thereby leading to rapid endothelialization of the implantation device surface and leading the implantation device to have excellent anticoagulant function and restenosis occurring resistance function.

Description

Technical field [0001] The invention relates to a biological modification method of titanium and titanium alloy cardiovascular implant devices. Background technique [0002] Cardiovascular diseases are one of the important diseases that endanger human health. Artificial implantation of titanium and titanium alloy cardiovascular implants (vascular stents or heart valves) is an effective method for the treatment of cardiovascular diseases. At present, more than 2 million patients with coronary heart disease in the world need percutaneous coronary angioplasty every year, and 70% of them need to be implanted with vascular stents. In 2006, more than 100,000 patients received vascular stent implantation in my country. Heart valve disease in my country accounts for about 30% of heart disease patients, and every year more than 100,000 critically ill patients must undergo artificial heart valve replacement. [0003] In 1987, Sigwart used titanium and titanium alloy intracoronary stents (B...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/06
Inventor 黄楠李全利陈佳龙陈诚陈俊英王进杨苹孙鸿冷永祥万国江赵安莎游天雪吴熹
Owner 深圳市金瑞凯利生物科技有限公司
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