Preparation method of 3D tissue engineering cell ring

A tissue engineering and cell technology, applied in the field of cell engineering, can solve the problems of geometric size gap, complicated operation process, scaffolds affecting cell growth and differentiation, etc.
CN110564671APending Publication Date: 2019-12-13吴宏伟 +1
4 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
吴宏伟
Publication Date
2019-12-13

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a preparation method of a 3D tissue engineering cell ring. By utilizing a relatively narrow growth space and a surface non-adhesion characteristic of a culture mold, added cells are self-assembled into a ring in a culture solution for promoting cell aggregation and leave the surface of a cell culture medium, and a macroscopic tissue block or organoid with a three-dimensional structure is formed. A naturally-formed extracellular matrix exists between the cells prepared by the method, the exogenous scaffold interference does not exist, and the communication and the information exchange between the cells are more complete and direct so as to reduce the difference between a culture environment and an in-vivo environment and more truly simulate an in-vivo cell growth environment. Moreover, the prepared cell ring is a cell body with a regular annular structure, can simulate the in-vivo cell microenvironment, links a 2D cell culture system with the research of tissuesand organs, and is beneficial to research of organoids.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of cell engineering, in particular to a method for preparing 3D tissue engineering cell rings. Background technique

[0002] Traditional medical research and cell biology research mostly adopt the two-dimensional cell culture model, which provides a flat culture plane. However, under physiological conditions, tissue cells grow in three-dimensional space, and cells not only contact each other, but also contact with supporting structures such as extracellular matrix. The two-dimensional cell culture model cannot really reflect the extracellular matrix in the tissue, so many physiological reactions in this model, such as receptor expression, transcription expression, cell migration and apoptosis, have been confirmed to be consistent with those that occur in actual organs or tissues. The situation is different. Moreover, the entire process of cell division, proliferation, migration and apoptosis is precisely regulated, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More