20 results about "Cell culture model" patented technology

The invention belongs to the technical field of biotechnology, and discloses a peanut peptide for promoting cell development, which is characterized in that the sequence of the peanut peptide is Met Ala Ser Val Thr Gly Pro Ser Ser Ala Leu Ala Asp Ser Ile Gly, through the accurately designed amino acid sequence, the peanut peptide constructs a molecular level adaptation mechanism with a cell surface receptor, triggers a cascade reaction of a key signal channel of phosphatidylinositol-3 kinase PI3K, and can promote cell development. The activation effect not only accelerates the process of a cell cycle and increases the proportion of DNA synthetic cells in an S phase by 40% or more, but also directionally induces the mesenchymal stem cells to differentiate into functional cells such as muscle cells and nerve cells by up-regulating transcription factors MyoD and NeuroD1, in a three-dimensional cell culture model, the cell migration rate of an experimental group added with 200 [mu] g / mL peanut peptide is increased by 58% compared with a control group, and the cell migration rate of the experimental group added with 200 [mu] g / mL peanut peptide is increased by 30% or more compared with that of the control group. The branch density of the formed vascular-like network is increased by 3 times, and meanwhile, the test further proves that the peanut peptide has the function of promoting cell development and shows remarkable optimization capability on a tissue regeneration microenvironment.

The present disclosure provides methods for generating hematopoietic progenitor cells. In some embodiments, the methods involve an in vitro or ex vivo cell culture model utilizing rentionic acid signaling for producing hematopoietic progenitor cells from pluripotent stem cells.

The invention discloses application of dicoumarin to resistance to eimeria infection, and belongs to the technical field of application of dicoumarin. The invention not only discloses that the biscoumarin can effectively inhibit the reproduction of the eimeria on an in-vitro eimeria cell culture model, but also discloses application of the biscoumarin in preparation of the eimeria infection resisting medicine or feed. According to the application, research finds that dicoumarin has an obvious inhibition effect on eimeria for the first time, the effect can be observed on an in-vitro eimeria cell culture model, and an in-vivo infection model of an animal with chicken infected with eimeria is verified. The application of the dicoumarin to the field of parasitic infection resistance is beneficial to expanding the medicinal value of the dicoumarin.

An assay plate system for assaying a cell culture model comprises (a) an assay plate comprising an upper surface and at least one row of wells, each well formed by a recess in the upper surface having an open top flush with the upper surface, a sidewall, and a closed transparent base, and (b) a gasket comprising a plate having a top surface, a bottom surface, and at least one elongated recesses formed in the bottom surface that together with the upper surface of the assay plate defines a fluidic conduit for the at least one row of wells when the upper surface of the assay plate and bottom surface of the gasket plate abut. The elongated recess comprises at least one projection that projects into one of the wells when the upper surface of the assay plate and bottom surface of the gasket plate abut, to modify fluid flow conditions in the well. The projection and the well are configured to provide a fluid flow path in the well all around the projection when the upper surface of the assay plate and bottom surface of the gasket plate abut.

The invention relates to an in vitro method of generating a murine cerebral organoid cell culture model, comprising cultivating murine neural stem cells obtained from an adult mouse until neurospheres have formed, and subsequently differentiating said neurospheres, thereby generating a murine cerebral organoid cell culture model. The invention further relates to an in vitro murine cerebral organoid cell culture model comprising mature astrocytes, oligodendrocytes and / or neurons produced according to the method of the invention and its use for drug or toxicity screenings or for studying neurological diseases. In other aspects the invention further relates to kits, cell culture media and supplement compositions comprising components to be used in the context of the present invention.

The present invention relates to the fields of cell transduction and transfection in a three-dimensional cell culture model and concerns, in particular, a specific cell microcompartment enabling transduction and / or transfection to be carried out within the internal part of said microcompartment.

The invention provides a culture medium, a culture method and application of an enameloblastoma cell. The culture medium comprises a culture medium I and a culture medium II, the culture medium I comprises DMEM (Dulbecco's Modified Eagle Medium) high glucose and the following components based on the volume of the DMEM high glucose: 10v% of Brazil fetal calf serum and 1% of penicillin-streptomycin solution; the culture medium II is prepared from advanced DMEM / F12, and the following components based on the volume of the advanced DMEM / F12: 10v% of Australian fetal calf serum, 5ng / ml of bFGF (basic fibroblast growth factor), 10ug / ml of Insulin, 10ng / ml of EGF (epidermal growth factor), 1% of penicillin-streptomycin solution, 2ug / ml of Hydrocotisone and 1.5 mM of N-acetal cysteine. The invention provides a high-efficiency and reliable oral maxillofacial region enameloblastoma cell culture medium and a culture method, successfully constructs an enameloblastoma primary cell culture model with good biological characteristics, provides a powerful tool for fundamental research, drug development and clinical application of enameloblastoma, and has a wide application prospect. And the method has obvious social and economic benefits.

Methods for producing extra-embryonic structures Described herein is a novel cell culture model of the post-gastrulation stage human amnion. Also described herein are methods for producing extra-embryonic structures in culture, in particular for producing an in vitro three-dimensional amnion model. The amnion models described herein, and amniotic membranes isolated from such amnion models, can be used in a variety of applications, such as in methods of drug screening, tissue engineering, regenerative medicine, wound healing and treating burns.

The invention discloses an integrated micro-fluidic platform for preclinical evaluation of a radiotherapy nano material and an evaluation method thereof, the platform comprises a G chip and a T chip which are cascaded, the G chip generates uniform matrigel liquid drops for encapsulating tumor cells through a flow focusing orifice, and tumor spheres with uniform size and high activity are formed through solidification and fluid perfusion culture; the T chip anchors a tumor sphere through an array type fixing structure, and forms a concentration gradient of a radiotherapy nanometer material through the regulation and control of a double-fluid channel, thereby achieving the precise pretreatment of the tumor sphere. The platform analyzes the activity and proliferation condition of tumor spheres through fluorescence imaging and immunofluorescence staining, and can quickly and reliably evaluate the curative effect of the radiotherapy nano material. The problems that a traditional 2D cell culture model is low in fidelity and an animal model is high in cost are solved, the method has the advantages of being easy and convenient to operate, low in cost and high in fidelity of simulated in-vivo tumor microenvironment, and a new scheme is provided for preclinical evaluation of radiotherapy nanometer materials.

A method for assaying the effects of a test agent on a microtissue comprises providing an assay plate system comprising an assay plate with a row of wells and a fluid conduit providing fluid communication along the row of wells, in each well contains a microtissue, flowing a test fluid containing the test agent along the fluid conduit and across each well, and imaging each of the microtissues in the wells The microtissue is disposed within a biopolymer hydrogel such as collagen or hyaluronic acid and is assayed in-situ in the biopolymer hydrogel in the well of the plate. The test agent may be an immune cell and the microtissue may be a cancer microtissue. The method allows for cells in the test fluid to attach to the hydrogel matrix and migrate into the hydrogel to interact with the microtissues held there. Entrapping a microtissue in a hydrogel within a well also allows for flow conditions of the test fluid in the well that are more physiologically relevant.

The invention relates to a Fibroblast Growth Factor 2 (FGF-2) polypeptides with improved stability while maintaining biological activity compared to the wild-type of FGF-2. The invention provides the process for the preparation, stabilisation and the use thereof in biotechnological research and industrial applications, the pharmaceutical industry, cosmetics, clean meat industry, generation of organoids, 3D cell culture models, in combination with physical modalities and other related applications.

The invention provides a construction and evaluation method for establishing a novel blood brain barrier model. The method solves the following technical problems: (1) the influence of the change of binding protein related factors on the blood brain barrier is proved by a time gradient, and the optimal time for opening and closing the blood brain barrier is found to confirm whether the influence of illumination on the blood brain barrier is instant opening and instant closing; (2) advantages and disadvantages of a single cell culture model and a co-cell culture blood brain barrier model; (3) finding out the optimal illumination time and intensity according to the change and toxicity of the illumination time and intensity on binding proteins and related factors; (4) black phosphorus is added, and the influence on cells and related factors is tested through illumination to serve as a control group of pure illumination time intensity. And a research idea related to the blood-brain barrier aspect is provided for treating neurological degenerative diseases.

A hybrid microextrusion pick-and-place 3D printing process enables fabrication of 3D-printed well plate-integrated piezoelectric cantilever sensors for monitoring properties and composition of tissue culture models. 3D-printed piezoelectric cantilever sensors exhibit multiple resonant modes across the 1-150 kHz frequency range. The ability to control cantilever frequency response (i.e., mode locations and types) was demonstrated by variation in the 3D-printed anchor geometry through corresponding changes in tool path and microextrusion process parameters. 3D-printed piezoelectric cantilever sensors facilitated in situ monitoring of cell culture media density and viscosity. This work presents an advance in the fabrication of cantilever sensors through the use of hybrid 3D-printing and pick-and-place processes that improves sensor reproducibility and enables novel control over anchoring of piezoelectric components and sensor integration with both 2D and 3D cell culture models, particularly 3D-bioprinted tissue constructs and microphysiological systems.

The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. The cellular model system for hepatocellular carcinoma (HCC) / cirrhosis development and progression may be used in the screening of compounds useful in the treatment and / or prevention of cirrhosis and / or HCC as well as in the identification biomarkers for the prediction of liver disease (especially cirrhosis) progression and HCC. The present invention also relates to specific compounds that have been identified, using such screening methods, as useful in the treatment and / or the prevention of HCC / cirrhosis.

The invention discloses a data fusion modeling system for airway epithelial barrier state evaluation, which comprises the following steps of: acquiring time sequence original data in a physiological microenvironment of an airway epithelial cell culture model, and processing to generate a synchronous multi-modal barrier time sequence data set; extracting and calculating multi-scale biophysical features capable of representing the structure and function of the epithelial barrier from the synchronous multi-modal barrier time sequence data set; constructing a multi-criterion weighted fusion model for generating a barrier health index; and comparing the generated barrier health index with a preset barrier health index state threshold, and outputting an evaluation report through a preset logic rule base. The method has the following advantages and effects that through multi-modal data fusion and multi-criterion weighted modeling, comprehensive and quantitative comprehensive evaluation on the structure and function state of the airway epithelial barrier is achieved, and the accuracy and early warning capacity of barrier health state monitoring are remarkably improved.

An assay plate system for assaying a cell culture model, such as an organoid, comprises an assay plate, an upper surface, a grid array of wells comprising at least two rows of wells, each row comprising at least two wells, each well having an open top formed in the upper surface and a closed transparent base, and at least two columns of wells, each column comprising at least three wells, each well having an open top formed in the upper surface and a closed transparent base. A first gasket comprising a plate having a top surface and a bottom surface comprising a plurality of elongated recesses is provided that together with the upper surface of the assay plate define a fluidic conduit for each row of wells providing fluidic communication between the first fluid inlet aperture, the row of wells and the first fluid outlet aperture in series.

The invention relates to an in vitro method for generating a neural organoid cell culture model. The present invention relates to an in vitro adherent neural organoid cell culture model comprising an organoid having a clear spatial structure and a regular eccentricity rate, the organoid comprising a core region comprising differentiated (mature) neurons, progenitor cells and glial cells and edges of neuronal and / or glial cell protrusions. The invention also relates to an in vitro neural organoid cell culture model produced according to the method according to the invention, to the use of the in vitro model according to the invention for high throughput drug or toxicity screening and / or for the study of nervous system diseases, to a kit for carrying out the method according to the invention, and to the use of the in vitro model according to the invention. To a cell culture plate or micropattern cell culture chip comprising an in vitro neural organoid model of the invention, and to a computer-implemented method for analyzing a neural organoid cell culture model of the invention.

The presently disclosed subject matter provides a microfluidic device that can simulate capillary blood flow on a fetal side of the device and pooled blood on a maternal side of the device (i.e., intervillous space). The microfluidic device can reconstitute the maternal-fetal interface, can expand the capabilities of cell culture models, and can provide an alternative to current maternal-fetal transfer models.

This invention discloses a data fusion modeling system for assessing the state of the airway epithelial barrier, comprising the following steps: collecting time-series raw data in the physiological microenvironment of an airway epithelial cell culture model, and generating a synchronous multimodal barrier time-series dataset after processing; extracting and calculating multi-scale biophysical features characterizing the structure and function of the epithelial barrier from the synchronous multimodal barrier time-series dataset; constructing a multi-criteria weighted fusion model to generate a barrier health index; comparing the generated barrier health index with a preset barrier health index state threshold, and outputting an assessment report through a preset logical rule base. This invention has the following advantages and effects: through multimodal data fusion and multi-criteria weighted modeling, this invention achieves a comprehensive and quantitative assessment of the structure and function of the airway epithelial barrier, significantly improving the accuracy of barrier health status monitoring and early warning capabilities.

The invention discloses a helicobacter pylori-related stomach organoid model and a construction method and application thereof, and relates to the technical field of stomach organoid model construction.The construction method comprises the steps that an in-vitro stomach tissue sample infected with helicobacter pylori is selected and subjected to digestion treatment, and stomach epithelial cells are obtained; mixing the gastric epithelial cells with matrigel to form a three-dimensional culture system; adding helicobacter pylori into the three-dimensional culture system, adding a culture medium, and carrying out co-culture; and regularly replacing the culture medium, and maintaining the growth and functions of the stomach organoid model. According to the helicobacter pylori related stomach organ model and the construction method thereof, through co-culture of a three-dimensional culture system and helicobacter pylori, the gastric mucosa inflammatory response after helicobacter pylori infection can be well simulated, and the helicobacter pylori related stomach organ model can be used for screening helicobacter pylori resisting drugs. Compared with a traditional two-dimensional cell culture model, the stomach organ model provided by the invention has higher physiological correlation and can more truly reflect an interaction mechanism between helicobacter pylori and gastric mucosa.