227 results about "Cell migration" patented technology

The invention provides recombinant IV type collagen and application thereof, and relates to the technical field of protein engineering. In the invention, an amino acid residue repetitive unit with good biological activity and stability in the human-derived IV-type collagen is found, and the amino acid residue repetitive unit is used as a core for repeating and constructing the recombinant IV-type collagen, so that the novel human-derived recombinant IV-type collagen with biological activity is obtained. The human-derived recombinant IV-type collagen provided by the invention is a small-molecular-weight protein, is easy to absorb by a human body, is good in safety, can remarkably promote secretion of collagen, also has the effects of promoting cell adhesion and cell migration, and has a good application prospect in the aspects of basement membrane repair and remodeling. A new functional component is provided for products for repairing and tightening the skin, resisting aging, resisting wrinkles, preserving moisture and the like.

The invention belongs to the field of biological medicine and material science, and particularly relates to an intelligent response type piezoelectric nanofiber dressing and a preparation method and application thereof. According to the preparation method, polyvinylidene fluoride-trifluoroethylene, a high polymer material and inorganic nanoparticles are blended and spun through an electrostatic spinning technology, and then a photo-thermal component coating is subjected to surface in-situ oxidation and self-polymerization, so that the composite fiber membrane is prepared. Under the stimulation of ultrasound (US) and near-infrared laser (Laser), the fiber membrane can cooperatively activate the piezoelectric effect and the photothermal effect, generate a dynamic electric field and local high temperature, realize multi-mode cooperative killing of bacteria (the antibacterial rate exceeds 99%), and simulate an endogenous electric field to promote cell migration and wound healing. The dressing has good mechanical properties (the Young modulus is 15-20 MPa), hydrophilicity (the contact angle is about 30 degrees), biocompatibility and stability, is suitable for treatment of chronic infected wounds such as diabetes mellitus and the like, and has the advantages of on-demand activation and intelligent response.

The invention discloses a gel microneedle loaded with silver nanoparticles and a preparation method and application thereof.The extract nano-silver (AB-AgNPs) is prepared through a green synthesis strategy, a drug-loaded microneedle system is constructed based on carboxymethyl chitosan-vanillic aldehyde-polyvinyl alcohol (CMCS-Va-PVA) composite gel, the AB-AgNPs prepared through the technology are uniform in particle size and stable in dispersion, and the drug-loaded microneedle has the advantages that the drug-loaded microneedle is good in drug-loaded performance and good in drug-loaded performance; the crystal has a typical face-centered cubic crystal structure and metal state Ag0 characteristics; the gel microneedle has good mechanical strength and forming effect, the AB-AgNPs and the gel matrix are stably combined through hydrogen bonds, the chemical properties are kept unchanged, and the system has broad-spectrum efficient bacteriostatic activity and biocompatibility; an in-vitro experiment proves that the green synthesized gel microneedle loaded with the silver nanoparticles has a remarkable capability of promoting the healing of L929 cell tissues; a rat wound experiment proves that the gel microneedle can regulate and control an inflammatory factor network and promote cell migration and tissue regeneration, healing of common and infected wounds is remarkably accelerated, and the wound residue ratio in 9 days is as low as 2.7% and 10.9% respectively.

The invention relates to the technical field of medical instruments, in particular to a photo-piezoelectric hydrogel dressing for wound repair. The hydrogel dressing realizes self-energized micro-electrical stimulation and anti-oxidation-antibacterial-anti-inflammatory integrated treatment through'energy-drug 'double closed-loop synergy: the PVDF / ZnO nanofiber on the upper layer can convert human body micro-motion and external light into micro-electrical stimulation to promote cell migration, proliferation and angiogenesis; the curcumin at the lower layer cooperates with the nano-silver to block the'oxidative stress-inflammation-infection 'vicious circle of chronic wounds. The dressing is soft and fit, does not need an external power supply, is excellent in biocompatibility, provides a self-driven multifunctional nursing scheme for refractory wounds, and has wide clinical popularization and market application prospects.

This invention discloses recombinant type III collagen, its preparation method, and its applications. The amino acid sequence of the recombinant type III collagen is shown in SEQ ID NO: 9. The recombinant type III collagen integrates the RGDV highly active motif and the EKEK hydrophilic stable domain with the core structure of type III collagen to achieve an integrated design of "high activity and long-lasting effect". The in vitro cell migration and collagen secretion effects are improved by more than 50% compared with wild type. Through anti-enzyme cleavage site modification and hydrophilic modification, the in vivo residence half-life is significantly prolonged to meet the long-term repair needs of the dermis.

The application discloses an active oxygen response type bacteriostatic and repair promoting hydrogel and a preparation method and application thereof, the hydrogel is formed by disulfide bond cross-linked hydrogel in response to active oxygen free radicals in the vagina of vaginitis patients through thiol modified hyaluronic acid (HASH), and can enhance in-vivo retention by thiol and vaginal tissue interaction. Cationic surfactant such as didecyldimethylammonium chloride (DDAC) as the bacteriostatic component of the hydrogel, efficiently killing pathogenic bacteria while having good biocompatibility, and is not prone to cause bacterial drug resistance. As a natural immune regulator, beta-glucan regulates the vaginal microenvironment, promotes the polarization of macrophages into anti-inflammatory M2 type and promotes cell migration, etc., to promote the repair of the vaginal immune barrier, regulate the vaginal flora, and reduce the probability of vaginitis recurrence. The HASH hydrogel provides an efficient, convenient and highly transformative potential strategy to provide more choices for the treatment of vaginitis.

This invention discloses an exovesicle from *Ashitsubaki* (Tomorrow's Leaf), its preparation method, and its application in the preparation of skin wound repair products, belonging to the field of plant exovesicle technology. The method uses fresh *Ashitsubaki* leaf stems and leaves as raw materials. After crushing, the material is filtered through a filter bag. The filtrate is then enzymatically hydrolyzed, centrifuged, and finally filtered through a tangential flow filtration system and further filtered to remove impurities and bacteria, yielding uniformly sized *Ashitsubaki* exovesicles. The preparation method of this invention is simple, and the obtained *Ashitsubaki* exovesicles exhibit excellent cell migration and skin healing promotion abilities, showing broad application prospects in the preparation of skin wound repair products.

The present invention relates to novel cell culture and transfection methods using a wildtype Caf1 ("Caf1-WT") polymer or a variant thereof. Use of a Caf1-WT polymer or a variant thereof to increase cell viability, cell proliferation, cell migration and / or cell clustering; promote the formation of a 3D cell structure; and / or promote suspension cell culture of cells that are normally adherent are also provided herein.

The invention discloses recombinant collagen III + X as well as a preparation method and application thereof, and belongs to the technical field of biochemical engineering. The amino acid sequence of the recombinant collagen III + X provided by the invention is shown as SEQ ID NO: 1, and the recombinant collagen III + X is formed by repeating human III type collagen 387-467 amino acid fragments twice and connecting human X type collagen 521-680 amino acid fragments to the C end. Experiments prove that the recombinant III + X collagen and proline hydroxylase are co-expressed to form a stable triple helix structure, and the recombinant III + X collagen has great advantages in the aspects of cell migration, safety and the like, and can be used as a raw material for preparing wound dressings and skin care products.

This invention relates to a vulnerary multifunctional composition, based on unripe coffee cherries (peel, mucilage, beans and silverskin), seed extract of Trigonella foenum-graecum, with one or more tissue-inducing molecules derived from the secondary metabolites of both plants. The unexpected and surprising ability of this new composition to solve the problems of chronic non-healing wounds addresses a long felt unmet need in the medical arts. Through a multifaceted approach, it combats microbial infections, reduces chronic inflammation, counters oxidative stress, enhances immune responses, promotes angiogenesis, regulates protease activity, stimulates collagen deposition, increases growth factor availability, facilitates cell migration, and prevents persistent infections. These novel integrated therapies represent a breakthrough in addressing macroscopic wound healing while targeting the underlying microscale mechanisms. This invention also relates to methods for preparing, using, and administering the multifunctional composition for timely healing in mammals with various skin lesions.

The present disclosure relates to CXCR3 ligands having CXCR3-expressing-cell migration-inducing activity, and specifically to amino acid modifications and amino acid sequences that are important for CXCR3-expressing-cell migration-inducing activity.

The utility model relates to a fine line device for an experiment for detecting cell migration ability, which comprises a fine line main body, the adhesive sheets are arranged at the two ends of the fine line main body, and adhesive protective layers are arranged on the adhesive sheets; and the two ends of the fine line main body exceed the adhesive sheet. The thin line main body with the structure can be conveniently adhered to a culture plate through the adhesive sheet and is also convenient to tear off, and a blank area is left after the thin line main body is torn off and is used for observing and recording the cell migration capability. The utility model provides a fine line device for an experiment for detecting cell migration ability, which is detachable, simple and convenient to operate, and capable of ensuring the consistency of scratch width and reducing damage to scratch edge cells and the bottom surface of a culture plate. The problems that scratches engraved by a gun head are inconsistent in width, and damage to cells and the bottom face of a culture plate is large are solved.

An object of the present invention is to provide a method for obtaining cells having a high migration ability or cells having a high proliferation ability as cells having an increased therapeutic effect on diseases. The present invention is a method for preparing cells for treating diseases, a method for promoting a migration ability of cells, and a method for promoting a proliferative ability of cells, each of the methods comprising subjecting cells to ultrasonic treatment. The frequency of the ultrasonic treatment is preferably 30 kilohertz to 2.0 megahertz, and the cells are preferably mesenchymal stem cells. In addition, the present invention also includes cells obtained by the preparation method of the present invention.

The invention provides a nucleic acid molecule, a mesenchymal stromal cell for promoting angiogenesis and application thereof, and relates to the technical field of biomedicine, the nucleic acid molecule encodes an HGF protein, or encodes a VEGF165 protein and an HGF protein; the first signal peptide is used as a signal peptide of VEGF165 protein, and the second signal peptide is used as a signal peptide of HGF protein. Through a gene modification strategy, the MSC cell containing the nucleic acid molecule stably and efficiently expresses VEGF165 and / or HGF, and the expression quantity of the VEGF165 and / or HGF is obviously higher than that of the MSC without the nucleic acid molecule. The capability of promoting endothelial cell migration is realized; meanwhile, the cells can also remarkably promote proliferation and tube formation of HUVEC cells, and the strong angiogenesis promoting effect of the cells is further proved. The technical problem that in the prior art, nucleic acid molecules for expressing VEGF165 or / HGF cannot be stably and efficiently expressed is solved.

This invention discloses recombinant humanized type III collagen, its preparation method, and its applications, belonging to the field of medical materials technology. The amino acid sequence of this recombinant humanized type III collagen is any one of SEQ ID No. 2-6. Compared to traditional extraction methods, the amino acid sequence of this recombinant protein is highly consistent with that of natural human proteins, completely avoiding the biosafety risks of pathogens, prions, etc., that may exist during animal-derived extraction, while also exhibiting lower immunogenicity. Furthermore, this protein effectively maintains the natural biological activity and stability of collagen. When used as a wound dressing, this material can efficiently promote cell migration, spreading, and proliferation, accelerate tissue regeneration and wound healing, while possessing excellent biocompatibility, controllable degradation, and low immunogenicity.

The invention relates to the field of biological medicine, in particular to a composition for repairing skin mucosa. The composition takes the cyclic peptide of Dap-Abu-Pro-Thz-Asp-Nva-Ser (PO3H2) and the vitamin B12 as active ingredients, the repair process of skin mucosa is remarkably promoted, including acceleration of epithelial cell migration, enhancement of barrier protein expression, inhibition of inflammatory response and promotion of wound healing, and compared with a traditional skin mucosa repair agent, the composition has higher repair efficiency and better treatment effect.

This application provides a method, system, electronic device, and storage medium for predicting wound healing progress based on 3D point cloud reconstruction, relating to the field of 3D point cloud reconstruction technology. This application generates spectral feature vectors containing biological indicators such as hemoglobin concentration and tissue oxygenation status by acquiring the reflectance spectral data and initial three-dimensional point cloud data of the wound. The initial point cloud is processed to obtain target data that retains its spatial morphology. The spectral feature values ​​are then fused point-by-point with the spatial coordinates of the target point cloud to generate a three-dimensional point cloud data cube. A 3D convolutional neural network is used to extract the spatial structural features within the cube, and the changes in spectral features at adjacent time points are tracked using optical flow, coupling the two. Based on the coupling results, a cell migration map is constructed, and the changing trend of node connection strength in the map is analyzed to obtain the predicted wound healing progress, which can improve the accuracy and comprehensiveness of wound healing progress prediction.

Provided is a coordination compound represented by formula (I). Also provided is a pharmaceutical composition that can be used to treat cancers, including the coordination compound of formula (I) and a pharmaceutically acceptable carrier. Also provided are applications of the coordination compound in inhibiting cancer cell survival or proliferation, inhibiting cancer cell migration or invasion, and treating cancerous tumors in a subject in need.

The invention discloses recombinant human III-type collagen and application thereof, and belongs to the technical field of biochemical engineering. The method comprises the following steps: according to human III-type collagen, obtaining a target amino acid sequence through splitting, and integrating the target amino acid sequence into an expression vector to obtain plasmids; transforming the plasmid into a host, and screening positive clones to obtain an expression strain; and culturing the expression strain, and separating and purifying to obtain the high-yield and high-purity human III-type collagen. The obtained recombinant human III-type collagen has a collagen triple helix region characteristic sequence (Gly-X-Y) n, has good performances of promoting cell migration and adhesion and preventing light damage, and can be widely applied to the fields of medicines, biological materials, health care products, cosmetics and the like.

The present application relates to the technical field of biotechnology, and provides a CRISPR-Cas9 system sgRNA sequence capable of efficiently targeting and knocking out a human SPARC gene, a recombinant vector containing the sgRNA sequence, and a construction method and application demonstration of a SPARC gene knockout cell model.The sgRNA sequence can mediate a high-specificity and high-efficiency SPARC gene knockout effect, effectively solving the problems of strong blindness, low screening efficiency and lack of reliable tool sequences in the prior art sgRNA design for the gene.The SPARC gene knockout Hela cell line constructed by using the sgRNA sequence and the recombinant vector has important technical value and practical significance for studying the extracellular matrix network regulation and cell migration ability.

This invention provides a platelet-rich plasma (PRP) sustained-release collagen sponge scaffold and its preparation method. The scaffold comprises: a first collagen sponge layer, a porous collagen sponge layer, and a second collagen sponge layer stacked sequentially; the porosity of the first and second collagen sponge layers is no more than 40%, and the pore size is no greater than 20 μm; the porosity of the porous collagen sponge layer is no less than 70%, and the pore size is no less than 50 μm; at least one sustained-release cavity is disposed in the porous collagen sponge layer for loading platelet-rich plasma gel. Through the structural design of the gradient pores of the collagen sponge and the internal sustained-release cavity, the outer layer forms a physical barrier to effectively prevent PRP loss; the inner layer efficiently stores PRP, ensuring the loading of effective components; the high porosity of the inner layer allows the PRP in the sustained-release cavity to slowly permeate outward, achieving long-term sustained release of PRP growth factors; at the same time, the pores of the inner layer can also provide sufficient space for cell migration and proliferation, promoting tissue regeneration.

The application discloses a pancreatic cancer early diagnosis marker button protein cingulin and an anticancer drug new target. The pancreatic cancer early diagnosis marker comprises an antibody of button protein CGN and a fluorescent quantitative polymerase chain reaction primer sequence, which accurately and clearly shows an expression mode and expression quantity change of the button protein CGN in a malignant tumor pancreatic cancer development process. The application discloses the expression mode of the button protein CGN in the early pancreatic cancer, and the button protein CGN is a new target for cell proliferation intervention and cell migration and invasion intervention. The button protein CGN is used as the diagnosis marker, and is favorable for early diagnosis of the pancreatic cancer; the button protein CGN is used as the target, and a drug for inhibiting tumor cell growth and metastasis can be developed, and a treatment effect on the tumor is enhanced.

An apparatus for guiding the migration of cancer and other cells includes a reservoir device, a cover, a tube, a nanofiber structure, and a lock device. The reservoir device defines a reservoir having an open top. The cover is configured for removable installation over the open top of the reservoir. The tube has a proximal end portion reaching into the reservoir. The nanofiber structure communicates an inlet port in the tube with the reservoir. The lock device interlocks the tube with the reservoir device, and also interlocks the nanofiber structure with the reservoir device.

The present application belongs to the technical field of biological medicine, and particularly relates to a tremella polysaccharide prepared by a low eutectic solvent method and application thereof in preparation of an anti-breast cancer drug. The present application first discovers that the tremella polysaccharide extracted by the low eutectic solvent method has a significant effect of treating breast cancer, and can resist breast cancer through inhibition of cell proliferation, inhibition of cell migration and invasion and other pathways. Compared with the tremella polysaccharide obtained by a traditional water extraction and alcohol precipitation method, the tremella polysaccharide obtained by the low eutectic solvent method has a lower total sugar content and weight average molecular weight, and a slightly higher protein content, and the tremella polysaccharide can not only significantly improve inhibition of breast cancer cell proliferation, but also significantly inhibit migration and invasion of breast cancer cells. This multi-target effect mode can effectively block the source of tumor diffusion, improve the treatment effect and the survival rate of patients, reduce the risk of recurrence and metastasis, and enhance the overall effect of treatment, and has a good application prospect in the treatment of breast cancer.

The invention discloses application of 2-fluorofucose in preparation of a medicine for relieving acute kidney injury, and belongs to the technical field of biology. In-vitro cell experiments and animal experiments prove that 2-fluorofucose can inhibit core fucosylation, competitively inhibit the activity of fucosyltransferase, down-regulate the expression of FUT8 and reduce the core fucosylation level in kidney tissues and renal tubular epithelial cells; by inhibiting core fucosylation, effectively reversing EMT phenotypic transformation induced by hypoxia reoxygenation or ischemia reperfusion, up-regulating E-cadherin expression, down-regulating alpha-SMA and Snail expression and reducing cell migration ability, phosphorylation activation of an ERK pathway is remarkably inhibited, and downstream inflammatory factor release and cell injury signal transduction are blocked, so that the ERK pathway can be used for inhibiting ERK pathway phosphorylation activation. The invention provides application of 2-fluorofucose to preparation of a medicine for relieving acute kidney injury. The invention provides a new choice for treating acute kidney injury, has the outstanding advantages of remarkable curative effect, high safety and the like, and has a wide application prospect.

The invention belongs to the field of medicines, and particularly relates to application of oseltamivir in a medicine for inhibiting porphyromonas gingivalis infected esophageal squamous cell carcinoma. According to the application of oseltamivir in the medicine for inhibiting the porphyromonas gingivalis infection type esophageal squamous cell carcinoma, tumor cell proliferation, cell migration and cell invasion of the porphyromonas gingivalis infection type esophageal squamous cell carcinoma can be inhibited.

This invention belongs to the field of biomedical functional materials technology, and relates to an antibacterial and healing-promoting photosensitizer, its preparation method, and its uses. The photosensitizer is a defective MIL-88B@MIL-88C composite material, wherein defective MIL-88B serves as the substrate, and MIL-88C grows on the surface and / or in the pores of the defective MIL-88B. This photosensitizer significantly improves the photogenerated carrier separation efficiency, reduces impedance, enhances photocurrent response, and can efficiently generate reactive oxygen species under light irradiation. Antibacterial experiments show that it has excellent bactericidal effects against Staphylococcus aureus, Escherichia coli, and methicillin-resistant Staphylococcus aureus, and is not prone to inducing drug resistance. Simultaneously, this photosensitizer has good biocompatibility, promotes cell migration, and has significant hemostatic function. Animal models show that it can effectively accelerate the healing of skin defects.

The invention relates to the technical field of microfluidics, and particularly discloses a cell communication micro-fluidic chip with a cell capturing structure and application of the cell communication micro-fluidic chip. The chip comprises main channels, and the two main channels are arranged in parallel at an interval; the cell interception structures are arranged in the main channels, grooves capable of accommodating at least one cell to be cultured are formed in the incident flow surfaces in the main channels, and at least one opening only allowing a culture solution to flow out is formed in each groove, so that the cells can stably stay and are uniformly distributed, and meanwhile, fluid is allowed to pass through; the culture solution interaction structure is arranged between the two main channels and used for interaction of culture solutions in the two main channels, transverse exchange of the culture solutions and cell secretions is achieved, cell migration is prevented at the same time, and therefore the non-contact cell communication model is constructed. The cell communication micro-fluidic chip can efficiently simulate the cell paracrine communication process, and is suitable for various cell communication research scenes such as inflammatory response, immune regulation, tumor microenvironment, stem cell secretion factor effect and the like.

The invention discloses an in-situ detection device for cell migration ability, comprising: a sealed box body, the top of which is provided with a transparent window; the microscope module is arranged in the sealed box body; the culture dish assembly is arranged outside the transparent window of the sealed box body; the liquid changing mechanism is used for automatically changing liquid for the culture dish assembly; the scratching mechanism is used for automatically realizing scratching operation; and the shaking table mechanism is used for automatically realizing cell rinsing operation. The invention also discloses an in-situ detection method for the cell migration ability. By constructing a full-automatic, high-fidelity and closed-loop integrated system from cell state sensing to final data output, four bottlenecks of a traditional cell scratch experiment in opportunity judgment, scratch creation, environment maintenance and process continuity are overcome at a time, and an ultimate solution is provided for realizing standardized and unmanned evaluation of the cell migration ability.

This invention belongs to the field of biomedical technology, specifically relating to the application of a functional lipid in the preparation of drugs for treating systemic lupus erythematosus (SLE). Specifically, the functional lipid MLnM exhibits significant therapeutic effects in a pristane-induced SLE mouse model, effectively alleviating SLE-related clinical symptoms, improving intestinal barrier function, reducing oxidative stress levels, regulating the balance of inflammatory cytokine secretion, and reducing systemic inflammatory responses by inhibiting the expression of immune cell migration-related genes. The discovery of MLnM provides a new direction for the treatment of SLE and other autoimmune diseases.