47 results about "Gelatin microspheres" patented technology

The invention provides a catechol functionalized gelatin microsphere and application of the catechol functionalized gelatin microsphere in preparation of a product for inhibiting formation of subcutaneous effusion and promoting tissue repair, and relates to the technical field of biological materials.According to the designed and prepared catechol functionalized gelatin microsphere, catechol groups are introduced into the gelatin microsphere, and the catechol functionalized gelatin microsphere is prepared; good liquid absorption performance and biodegradability are realized. Cell experiment results show that the catechol functionalized gelatin microspheres have super multiple interfaces and can promote cell adhesion and proliferation, and the loose structure of the catechol functionalized gelatin microspheres can promote cell migration. In-vivo rabbit chest wall defect repair experiment results show that the catechol functionalized gelatin microspheres not only can inhibit formation of subcutaneous effusion, but also can collect autologous cells in situ and promote proliferation of the autologous cells and secretion of extracellular matrixes (such as collagen). The results show that the catechol functionalized gelatin microsphere material has clinical application potentials of inhibiting formation of subcutaneous effusion and promoting healing of subcutaneous wounds.

The invention relates to the field of hydrogel materials, in particular to a hydrogel dressing as well as a preparation method and application thereof. The deferoxamine mesylate is loaded on the gelatin microsphere material constructed by the gelatin and the genipin, so that the loading dispersion of the deferoxamine mesylate can be benefited, and the added gelatin can be in contact with matrix proteinase-9 in a diabetic foot ulcer microenvironment for degradation so as to induce the release of the deferoxamine mesylate. A first cross-linked network is formed by the 3-carboxyphenylboronic acid modified chitosan and the oxidized hyaluronic acid, and the gelatin microsphere material loaded with the deferoxamine mesylate and the epigallocatechin gallate are coated with the first cross-linked network. The hydrogel dressing realizes controllable release of the deferoxamine mesylate and the epigallocatechin gallate, so that the technical problem of uncontrollable release of the deferoxamine mesylate is solved.

The invention discloses a preparation method and application of a double-layer sound transmission type berberine-loaded gelatin microsphere slow-release antibacterial gel dressing, and belongs to the technical field of gel dressings. The preparation method comprises the following steps: taking vinyl silicone oil and silicon dioxide as raw materials, preparing upper-layer silica gel by adopting a double-component uniform mixing method, and then laminating the upper-layer silica gel and lower-layer berberine-loaded gelatin microsphere hydrogel by adopting a self-laminating method, thereby obtaining the double-layer sound-transmission type berberine-loaded gelatin microsphere slow-release antibacterial gel dressing. By means of the broad-spectrum antibacterial function of berberine, the efficient drug loading function and slow drug release function of gelatin microspheres, the sound transmission and swelling functions of lower hydrogel and the supporting function of upper hydrogel, ultrasonic inspection is conducted through a dressing covering layer, an ultrasonic scanning supporting plane is provided, ultrasonic waves penetrate through the dressing, good imaging is formed, and the application prospect is wide. And antibacterial and repairing effects on local wounds are realized at the same time.

The invention discloses injectable double-drug delivery hydrogel and a preparation method thereof, and belongs to the technical field of biomedical materials. According to the method, two nano microspheres with different charges are respectively prepared by utilizing the characteristics of A-type gelatin and B-type gelatin, a negatively charged medicine EGCG is specifically carried by the positively charged A-type gelatin, and a positively charged medicine SAG is carried by the negatively charged B-type gelatin microsphere; and finally, mixing the two gelatin drug-loaded microspheres into a material taking gelatin and zirconium ions as a substrate to form the composite hydrogel. The hydrogel realizes immune regulation and osteogenesis regulation, also realizes long-acting release of the two drugs, effectively solves the problems of bone resorption, difficulty in healing and the like of host tissues through synergistic regulation of inflammation and mobilization of osteogenesis genes, realizes personalized treatment, and improves the clinical treatment effect and efficiency.

The invention discloses an antibacterial seaweed composite fiber and a preparation method thereof, and relates to the technical field of seaweed fibers.The antibacterial seaweed composite fiber is prepared from, by weight, 50-60 parts of alginate, 10-20 parts of silk fibroin, 10-20 parts of polyvinyl alcohol and 1-3 parts of nano-silver-loaded porous gelatin microspheres; the mass ratio of the nano-silver to the porous gelatin microspheres in the nano-silver-loaded porous gelatin microspheres is 1: (60-70). The composite fiber can effectively improve the mechanical property and antibacterial property of the alginate fiber.

The invention provides a bionic epiphyseal plate and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The gelatin microspheres are used as cell carriers, the surface area is large, and cells can obtain sufficient nutrition support; the method comprises the following steps: by taking hMSC microspheres as a basic construction unit, carrying out static incubation under the stimulation of recombinant IHH protein, and driving the hMSC microspheres to spontaneously aggregate, fuse and self-assemble into the bionic epiphyseal plate of which internal cells are in a proliferation and differentiation balance state without depending on a mold and an external force condition. The cell state, matrix morphology and mechanical properties of the biomimetic epiphyseal plate are similar to those of a natural epiphyseal plate, the biomimetic epiphyseal plate has obvious supporting effect on cells and adaptability to mechanical microenvironment of injury sites, has dual functions of preventing bone bridges and promoting cartilage regeneration, and can be used as a candidate filler for repairing large-area epiphyseal plate defects. Compared with a traditional method for preparing a epiphyseal plate replacement scaffold, the method has higher cartilage formation induction efficiency, and the induction period is shortened.

The invention belongs to the field of biomedical materials and tissue engineering, and particularly relates to an injectable mineralized collagen bone repair composite material loaded with stem cell recruitment factors and osteogenic induction factors and a preparation method of the injectable mineralized collagen bone repair composite material. The composite material is composed of 50%-53% of mineralized collagen matrix particles, 15%-20% of SDF-1alpha loaded heparinized gelatin microspheres, 15%-20% of BMP-2 loaded PDA coated mesoporous silica particles, 8%-10% of an NHS-PEG-NHS cross-linking agent and 4%-6% of glycerin. The composite material can realize time-space sequential regulation and control of'collection first and then differentiation '; a bionic bone matrix microenvironment is provided through the mineralized collagen matrix; the problems of two-factor release conflict and stability are solved; in-vitro experiments show that the material can significantly promote stem cell migration and osteogenic differentiation, and animal experiments prove that the material can recover 82-88% of mechanical properties of bone defect parts. The invention solves the technical problems of insufficient stem cell collection, low osteogenic differentiation efficiency and uncontrollable factor release of the existing bone repair material.

The invention relates to the technical field of biomedical materials, in particular to a method and equipment for preparing porous gelatin microspheres. The method for preparing the porous gelatin microspheres is realized by utilizing a micro-fluidic chip with a micro-mixing area and a micro-dispersion area and a curing system, and comprises the following steps: injecting gelatin derivative fluid and polymer fluid into the micro-mixing area at 30-60 DEG C, so that the gelatin derivative fluid and the polymer fluid flow and are mixed in the micro-mixing area, and the micro-mixing area is formed; and carrying out time sequence phase separation and self-assembly under the periodic influence of the microstructure of the micro-mixing region to form a micro-mixing fluid, and carrying out micro-dispersion and curing to obtain the porous gelatin microspheres. Loading a load into the fluid solution to prepare the porous gelatin microspheres containing the load. According to the method, the microsphere industrialization process of the porous gelatin material is simplified, the nano-structure characteristics of the porous gelatin microspheres can be efficiently reserved, the quality of the nano-structure porous gelatin microspheres is improved, and efficient development, batch production and application of porous gelatin products can be accelerated and promoted.

The present invention relates to the technical field of joint repair hydrogels, and in particular to a three-dimensional biohydrogel and its preparation method and application. The three-dimensional biohydrogel, by weight, comprises: 1-5 parts of methacrylic anhydride hyaluronic acid HAMA, 0.05-0.15 parts of initiator, 3-8 parts of hydrogenated soybean phosphatidylcholine liposomes and 7-13 parts of stem cell solution; the preparation method of the stem cell solution is: gelatin and water are mixed to obtain a gelatin solution; stirring at 40-60 ° C to prepare a dispersed phase; adding an emulsifier to the oil to prepare a continuous phase; the dispersed phase and the continuous phase are prepared by microfluidic technology to obtain uniform droplets, and the droplets are freeze-dried to obtain gelatin microspheres; stem cells are added to the gelatin microspheres and incubated at 25-37 ° C for 2-4 hours to obtain a stem cell solution. The present invention has long-lasting efficacy and significant safety, and can fundamentally repair articular cartilage.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses sequential slow-release temperature-sensitive hydrogel and a preparation method and application thereof.The preparation method comprises the following steps that a blood sample is subjected to programmed centrifugation, and a gel layer of leukocyte-rich platelet-rich fibrin L-PRF and a gel layer of improved platelet-rich fibrin A-PRF are obtained respectively; and the gel layer is cut into pieces, pre-frozen, freeze-dried, ground and sieved, so that L-PRF freeze-dried powder and A-PRF freeze-dried powder are obtained respectively, and the methacrylic acid gelatin microspheres entrapped with the L-PRF freeze-dried powder are prepared. The microspheres and A-PRF freeze-dried powder are loaded into a temperature-sensitive hydrogel system composed of chitosan, polygalacturonic acid and sodium beta-glycerophosphate, so that the growth factors can be effectively entrapped in the hydrogel structure, and continuous release is realized; the system has good mechanical strength and structural stability, the retention time of the material in vivo is prolonged, and stable three-dimensional network support is provided.

The invention relates to the technical field of interventional medicine, in particular to sulfonated gelatin microspheres and a preparation method thereof. The preparation method comprises the following steps: adding gelatin into a buffer solution, heating, stirring and dissolving to obtain a water phase, adding a dispersing agent into an oil phase matrix solution, stirring to obtain an oil phase, transferring the water phase into the oil phase, stirring, and cooling to obtain gel microspheres; the preparation method comprises the following steps: primarily cleaning gel microspheres, putting the gel microspheres into a freezing medium for freeze drying, then adding the freeze-dried gel microspheres into normal saline, adding a cross-linking agent for a cross-linking reaction, then adding a sulfonation reagent, a catalyst and an initiator for a sulfonation reaction, and finally, carrying out secondary cleaning on the microspheres after the sulfonation reaction is finished, so as to obtain the hydrogel. The sulfonated gelatin microspheres are obtained. According to the preparation method, the cross-linked microspheres are firstly prepared, then the cross-linked microspheres are subjected to sulfonation treatment, the reaction conditions are mild, the process is simple, amplified production is easier to perform, the microspheres (45 [mu] m) with very small particle sizes can be obtained, and the adhesion condition is avoided.

The invention belongs to the technical field of biomedical materials. The invention provides microspheres for promoting Ext1 expression and relieving osteoarthritis and a preparation method of the microspheres. The microspheres comprise gelatin microspheres and bone marrow mesenchymal stem cells loaded on the microspheres. In the field, the effects of promoting Ext1 gene expression and relieving osteoarthritis are achieved for the first time under the condition that exogenous drugs are not adopted; meanwhile, the action time of the invention can be as long as 30 days. The invention provides a new direction for clinical treatment of osteoarthritis in the future, has huge potential in the aspects of personalized and non-surgical treatment, can reduce dependence on drugs and improve joint health, and provides an economical and practical solution.

The application discloses a kind of degradable microspheres with controllable and preparation method thereof, belong to the technical field of biomedical materials.The base material of the microspheres is methacrylated gelatin, and the degradation time of the microspheres is controlled by regulating the degree of substitution of methacrylated gelatin.The microspheres are prepared by microfluidic and photopolymerization technology, and have uniform size with particle size of 10 μm to 1000 μm.The methacrylated gelatin microspheres with controllable degradation provided by the application can be used for interventional recanalization embolization therapy of substantial organ hemorrhage, realize the time sequence regulation of "hemostasis-degradation-recanalization", and have good clinical transformation value.

The invention discloses an antibacterial gelatin microsphere with a photodynamic effect and a preparation method and application of the antibacterial gelatin microsphere, and belongs to the technical field of biomedical materials and hemostasis. An olefinic unsaturated carboxylic acid monomer is grafted to a gelatin side chain through amidation reaction to obtain modified gelatin; the modified gelatin and a cross-linking agent free radical are subjected to a copolymerization reaction to form carboxyl functionalized gelatin microspheres, and a photosensitizer is covalently immobilized on the surfaces of the carboxyl functionalized gelatin microspheres through an esterification reaction to prepare the antibacterial gelatin microspheres with the photodynamic effect. According to the antibacterial gelatin microspheres with the photodynamic effect and the preparation method and application of the antibacterial gelatin microspheres, the efficiency and stability of subsequent photosensitizer loading can be remarkably improved, the postoperative infection risk is effectively solved, and the limitation that a traditional hemostatic material only pays attention to physical plugging is broken through.

The invention provides a simulated thrombus and a preparation method thereof. The preparation method comprises the following steps: preparing a polymer matrix solution; preparing gelatin microspheres and composite particles, wherein the composite particles are iron oxide particles coated with polydopamine on the surfaces; mixing the gelatin microspheres and the composite particles with the polymer matrix solution to form a composite dispersion liquid; performing network forming treatment and crosslinking treatment on the composite dispersion liquid to obtain a thrombus precursor; soaking the thrombus precursor in a PBS (Phosphate Buffer Solution) to balance the water content; and performing cyclic compression treatment on the thrombus precursor to obtain the simulated thrombus.

The present disclosure relates to the technical field of biomaterials, and provides a catechol-functionalized gelatin microsphere and a use thereof in preparation of a product for inhibiting formation of subcutaneous fluid accumulation and promoting tissue repair. The catechol-functionalized gelatin microsphere designed and prepared in the present disclosure not only has wet tissue adhesion capability, but also has good liquid absorption performance and biodegradability. The results of cell experiments show that abundant interfaces of catechol-functionalized gelatin microspheres can promote cell adhesion and proliferation, and the loose structure of the microspheres can also promote cell migration. The results of chest wall defect repair experiments of New Zealand white rabbits show that the catechol-functionalized gelatin microspheres can not only inhibit formation of subcutaneous fluid accumulation, but also recruit autologous cells in situ and promote proliferation thereof and secretion of extracellular matrix (such as collagen). These results indicate that the catechol-functionalized gelatin microsphere material has clinical application potential of inhibiting the formation of subcutaneous fluid accumulation, and promoting subcutaneous tissue regeneration and wound healing.

The invention discloses preparation and application of a double-enzyme-driven stage response type external preparation, which comprises the following steps: preparing drug-loaded hyaluronic acid (HA) microspheres, namely constructing emodin (EMD)-loaded hyaluronic acid microspheres (EMD-HA microspheres) for a responsive traditional Chinese medicine external preparation; preparation of hyaluronidase (HAase) gelatin microspheres; preparing a medicine-containing ointment substrate; and carrying out magnetic stirring or mechanical homogeneous stirring on the obtained EMD-HA microspheres, HAase gelatin microspheres and an ointment matrix for 30 minutes at room temperature on the basis of a dosage ratio, so that the microspheres are fully and uniformly dispersed in the ointment matrix, thereby obtaining the dual-enzyme driven stimulus-responsive external preparation. According to the preparation disclosed by the invention, 'targeted response 'treatment aiming at different stages of rosacea is realized; the convenience and compliance of treatment are improved, and meanwhile, individualized precise treatment for rosacea is realized.

The invention relates to the field of medical micro-nano robots, in particular to a magnetic-driven dual-core micro-nano robot for targeted therapy of colorectal cancer and a using method thereof.The micro-nano robot is provided with a calcium alginate outer membrane, the micro-nano robot is provided with a water-drop-shaped tail structure, and gelatin microspheres A and gelatin microspheres B are arranged in the calcium alginate outer membrane; the method comprises the steps that firstly, the magnetic drive micro-nano robot penetrates through the gastric acid environment to reach the intestinal tract; 2, controlling the micro-nano robot to stop in the lesion area through a magnetic field, and breaking the micro-nano robot to release the gelatin microspheres A and the gelatin microspheres B; 3, sequentially breaking the gelatin microspheres A and the gelatin microspheres B through a rotating magnetic field; the three technical bottlenecks of gastric acid inactivation of oral medicines, low mucus penetration efficiency and multi-medicine synergistic sequential control are overcome.

The invention discloses a PDA (at) Gel (at) GO nano-microcapsule for targeting colitis as well as a preparation method and application of the PDA (at) Gel (at) GO nano-microcapsule, and particularly relates to research on relieving colitis on the basis of a galactosylated carboxymethyl chitosan nano-micelle oral micro-capsule delivery drug system. According to the preparation method, a Gal-N-CMCS / Oridonin (GO) nano-micelle formed by loading Oridonin in a self-assembly manner by taking Gal-N-CMCS as a framework is constructed, then the Gal-N-CMCS / Oridonin (GO) nano-micelle is embedded into gelatin microspheres, and the gelatin microspheres are coated with dopamine to prepare the PDA / Gel / GO nano-microcapsule. Oral delivery and colon targeted release of drugs can be achieved, by inhibiting NLRP3 inflammasome activation and promoting epithelial cell regeneration, DSS-induced colitis and mucosal lesion are effectively relieved, and a new strategy and mechanism basis are provided for oral precise treatment of colitis.

The invention belongs to the field of carbon materials, and particularly relates to tungsten-doped gelatin-based carbon spheres as well as a preparation method and application thereof. Biomass raw material gelatin is adopted as a raw material, gelatin microspheres are prepared through an emulsion method, meanwhile, tungsten element doping is carried out, and then the tungsten-doped gelatin-based carbon spheres are obtained through carbonization treatment. And compared with the traditional carbon material, the raw materials are green and environment-friendly, the generated pollution is relatively low, the cost is low, and the advantages are obvious. The process for preparing the carbon spheres is relatively mild in reaction condition, simple and convenient to operate and free of discharge of other waste substances, belongs to an environment-friendly process route, and the prepared carbon spheres as an electrode material have good electrochemical performance and have great application prospects in the fields of electrochemistry and supercapacitors.

The present invention provides a kind of acetate fabric low temperature dyeing auxiliary agent, the dyeing auxiliary agent includes the following ingredients: polyoxyethylene ether polyoxyethylene ether, sodium lignin sulfonate, peregal O 20, acetic acid, dispersant QTQ 108, porous gelatin ball and chitosan;The concentration of the dyeing and finishing auxiliary agent is:polyoxyethylene ether polyoxyethylene ether 1.8 2.5g / L, sodium lignin sulfonate 0.2 0.45g / L, peregal O 20 is 0.05 0.1g / L, acetic acid 5 10g / L, dispersant QTQ 108 is 0.5 1.2g / L, porous gelatin ball 10 20g / L, chitosan 2 5g / L, and the molecular weight of chitosan is 2000 5000Da. The present invention improves the coloring effect and the color fixing effect of acetate fiber dyeing at lower temperatures by adding modified gelatin microspheres in printing and dyeing auxiliary agents.

The present application relates to the field of biological materials, in particular to a preparation method and application of GMS@EVs microspheres loaded with SNAP-VHH fusion protein. The present application constructs a gelatin microsphere delivery system (GMS@EVs) with capture and slow-release functions. The system specifically and efficiently captures CD9-positive exosomes secreted by mesenchymal stem cells through the stable fixation of SNAP-VHH fusion protein on the surface of gelatin microspheres, and realizes the efficient extraction of CD9-positive exosomes from the culture supernatant of mesenchymal stem cells. The present application provides specific reaction sites for the fixation of SNAP-VHH fusion protein by grafting O6-[4-(aminomethyl)benzyl]guanine on the gelatin microspheres, so that the SNAP-VHH fusion protein is stably loaded on the gelatin microspheres. Through experiments, the GMS@EVs microspheres prepared by the present application have controllable slow-release properties for exosomes, and still maintain significant fluorescence signals 48 hours after subcutaneous injection in nude mice, which is at least 16 times longer than the slow-release time of free exosomes (30 minutes of rapid diffusion), realizing the sustained release of exosomes in the local wound and enhancing the durability of the therapeutic effect.

The invention belongs to the technical field of three-dimensional culture and delivery of cells, and discloses gelatin / hyaluronic acid porous hydrogel composed of a bionic extracellular matrix and used for three-dimensional culture and delivery of dendritic cells and a preparation method of the gelatin / hyaluronic acid porous hydrogel. The method comprises the following steps: taking a semi-interpenetrating polymer network composed of methacrylic anhydride modified gelatin (GelMA) and oxidized hyaluronic acid (OHA) as a matrix, introducing temperature-responsive gelatin microspheres and dendritic cells at the same time, then raising the temperature until the gelatin microspheres are melted, and constructing a three-dimensional supramolecular hydrogel cell in-vitro culture model with decoupled aperture and rigidity. The supramolecular hydrogel has the advantages of convenience in use, simplicity in operation, biosafety and the like, and can be applied to the fields of three-dimensional culture and delivery of dendritic cells, tumor immunotherapy and the like.

The invention discloses a thalassemia treatment method based on a stem cell technology and application. The thalassemia treatment method comprises the following steps: collecting a peripheral blood sample of a patient, and carrying out stem cell isolated culture; constructing a nucleic acid aptamer by combining a vascular cell adhesion molecule-1 and a stromal cell-derived factor-1 in a bone marrow microenvironment; connecting a nucleic acid aptamer, a cell adhesion promoting peptide and a stem cell growth factor, and constructing a homing guiding reagent; preparing nano liposome and nano polymer particles; the nucleic acid aptamer is connected to the surfaces of the nano-liposome and the nano-polymer particles, and is grafted with a temperature-responsive material to obtain the nano-carrier with a responsive release function; stem cell growth factors and platelet-derived growth factors are mixed with gelatin microspheres to form a slow release system; the cultured stem cells are mixed with a homing guide reagent, a nano-carrier and a slow release system, and are transplanted into the body of a patient through intramedullary injection. The stem cell homing efficiency can be improved, and the treatment effect on thalassemia is improved.

The invention discloses a traditional Chinese medicine composition for treating asthma and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 2-10 parts of pericarpium citri reticulatae, 2-10 parts of roses, 2-10 parts of sword beans, 2-10 parts of pinellia ternate, 4-20 parts of scaphium scaphigerum, 4-20 parts of semen armeniacae amarae, 4-20 parts of radix stemonae, 4-20 parts of folium eriobotryae, 3-16 parts of polygala japonica, 3-16 parts of folium mori and 40-200 parts of lean pork. The composition disclosed by the invention has an obvious effect of treating asthma; in addition, the gelatin-containing microspheres are used for carrying out tannin removal treatment on roses and folium eriobotryae in the traditional Chinese medicine composition to reduce the influence of tannin components on other medicinal components, and the gelatin microspheres subjected to tannin removal are recycled and applied to bitter apricot kernel processing, so that the nutrition loss can be reduced while the enzyme deactivation and glycoside protection effects are improved; the asthma treatment effect of the composition is comprehensively improved; on the basis, the taste of the traditional Chinese medicine composition is improved, a specific flavoring agent is added during preparation of the composition, and the traditional Chinese medicine composition with good taste is obtained.

The invention discloses a composite thermosensitive hydrogel embolism material and a preparation method thereof, and belongs to the technical field of biological medicines. The invention aims to solve the problems of insufficient strength, uncontrollable gel forming and development deficiency of the embolism material. The cleaning agent is composed of the following components in percentage by weight: 5 wt% of sodium alginate; 20 wt% of a developer; 8-15 wt% of a crosslinking triggering agent gelatin microsphere; 1.5 to 2 wt% of a thixotropic agent; and the balance of sterilized water for injection. The molar ratio of Ca to G is 0.3 to 0.35. At room temperature, lithium magnesium silicate forms a physical gel network, so that the material is endowed with a shear thinning characteristic, and the material can be smoothly injected through a microcatheter and stably suspends a developing agent; when the gelatin microspheres are injected into in-vivo target blood vessels at 37 DEG C, the gelatin microspheres generate thermal response phase change, released calcium ions trigger sodium alginate to generate irreversible ionic crosslinking, a high-strength chemical gel network is formed, and the gelatin microspheres are suitable for treatment of vascular diseases such as liver cancer interventional embolism and the like.

The application belongs to the technical field of three-dimensional culture and delivery of cells, and discloses a gelatin / hyaluronic acid porous hydrogel composed of a biomimetic extracellular matrix for three-dimensional culture and delivery of dendritic cells and a preparation method thereof. The method uses a semi-interpenetrating polymer network composed of methacrylic anhydride modified gelatin (GelMA) and oxidized hyaluronic acid (OHA) as a matrix, introduces temperature-responsive gelatin microspheres and dendritic cells at the same time, and then constructs a three-dimensional supramolecular hydrogel cell in-vitro culture model with decoupled pore size and stiffness after the gelatin microspheres are melted by increasing the temperature. The supramolecular hydrogel has the advantages of convenient use, simple operation, biological safety and the like, and can be used in the fields of three-dimensional culture, delivery and tumor immunotherapy of dendritic cells.

The invention discloses a multifunctional gelatin microsphere and a preparation method and application thereof, the multifunctional gelatin microsphere is prepared from gelatin, tea hair and an oxidation initiator as raw materials, and the multifunctional gelatin microsphere has multiple biological functions of water absorption, oxidation resistance, hemostasis, photo-thermal and the like. The preparation method comprises the following steps: filling an injector with a water-phase prefabricated liquid containing gelatin and tea hair, delivering the prefabricated liquid into an oil phase by a syringe needle through vertical reciprocating motion, cutting the prefabricated liquid into micro-droplets with uniform sizes through shearing force of a liquid-liquid interface and tension of an oil / water interface when the syringe needle leaves the oil phase, and solidifying the micro-droplets in the oil phase, so as to obtain the gelatin / tea hair-containing micro-droplets. And initiating crosslinking of the micro-droplets through an oxidation initiator to form the gelatin microspheres. The diameter of the microsphere is controlled by adjusting the injection rate of the prefabricated liquid, the reciprocating motion frequency of the needle and the size of the needle, the preparation process is simple, the diameter is controllable, and the cost is low. The multifunctional gelatin microspheres can be used in the biomedical fields of hemostasis, prevention of postoperative adhesion, promotion of tissue repair and the like.

The invention provides a gelatin microsphere modified by beta-cyclodextrin, a gelatin microsphere modified by deferoxamine loaded beta-cyclodextrin as well as a preparation method and application of the gelatin microsphere, and relates to the technical field of biological materials. Beta-cyclodextrin modified gelatin microspheres with good thermal stability and deferoxamine (DFO) drug loaded beta-cyclodextrin modified gelatin microspheres are successfully designed and prepared by adopting specific materials. Cell experiment results show that the two compounds have good cell compatibility and can promote adhesion, proliferation and migration of cells. The slow release of DFO in the deferoxamine drug loaded beta-cyclodextrin modified gelatin microspheres can promote the vascularization effect of HUVECs cells. The results of rat abdominal wall defect repair experiments show that the microsphere scaffold provided by the invention has obvious advantages in promoting abdominal wall tissue reconstruction, and shows huge clinical application potential.

The invention relates to the field of biological medicine, and discloses a medicine suspension composition for improving osteoarticular diseases and a preparation method thereof. In the embodiment 1, the composition comprises sterile normal saline and umbilical cord mesenchymal stem cell-loaded modified gelatin microspheres with the volume fraction of 50%; the microspheres are prepared from phenylboronic acid-sulfobetaine bifunctional modified gelatin, 0.2 M trehalose, a photoinitiator and stem cells through a microfluidic technology. The modified gelatin is grafted with 3-carboxyphenylboronic acid through an amido bond, and is grafted with a sulfydryl-containing sulphobetaine derivative through Michael addition; the cartilage anchoring and active oxygen removal are realized by utilizing phenylboronic acid groups, agglomeration is prevented by utilizing a sulphobetaine hydration layer, cells are protected by matching with trehalose, and the cartilage hydrogel has high dispersity, high survival rate and excellent tissue adhesion.