24 results about "Gelatin microspheres" patented technology

The invention discloses injectable double-drug delivery hydrogel and a preparation method thereof, and belongs to the technical field of biomedical materials. According to the method, two nano microspheres with different charges are respectively prepared by utilizing the characteristics of A-type gelatin and B-type gelatin, a negatively charged medicine EGCG is specifically carried by the positively charged A-type gelatin, and a positively charged medicine SAG is carried by the negatively charged B-type gelatin microsphere; and finally, mixing the two gelatin drug-loaded microspheres into a material taking gelatin and zirconium ions as a substrate to form the composite hydrogel. The hydrogel realizes immune regulation and osteogenesis regulation, also realizes long-acting release of the two drugs, effectively solves the problems of bone resorption, difficulty in healing and the like of host tissues through synergistic regulation of inflammation and mobilization of osteogenesis genes, realizes personalized treatment, and improves the clinical treatment effect and efficiency.

The invention relates to the technical field of biomedical materials and tissue engineering, and discloses sequential slow-release temperature-sensitive hydrogel and a preparation method and application thereof.The preparation method comprises the following steps that a blood sample is subjected to programmed centrifugation, and a gel layer of leukocyte-rich platelet-rich fibrin L-PRF and a gel layer of improved platelet-rich fibrin A-PRF are obtained respectively; and the gel layer is cut into pieces, pre-frozen, freeze-dried, ground and sieved, so that L-PRF freeze-dried powder and A-PRF freeze-dried powder are obtained respectively, and the methacrylic acid gelatin microspheres entrapped with the L-PRF freeze-dried powder are prepared. The microspheres and A-PRF freeze-dried powder are loaded into a temperature-sensitive hydrogel system composed of chitosan, polygalacturonic acid and sodium beta-glycerophosphate, so that the growth factors can be effectively entrapped in the hydrogel structure, and continuous release is realized; the system has good mechanical strength and structural stability, the retention time of the material in vivo is prolonged, and stable three-dimensional network support is provided.

The invention relates to the technical field of interventional medicine, in particular to sulfonated gelatin microspheres and a preparation method thereof. The preparation method comprises the following steps: adding gelatin into a buffer solution, heating, stirring and dissolving to obtain a water phase, adding a dispersing agent into an oil phase matrix solution, stirring to obtain an oil phase, transferring the water phase into the oil phase, stirring, and cooling to obtain gel microspheres; the preparation method comprises the following steps: primarily cleaning gel microspheres, putting the gel microspheres into a freezing medium for freeze drying, then adding the freeze-dried gel microspheres into normal saline, adding a cross-linking agent for a cross-linking reaction, then adding a sulfonation reagent, a catalyst and an initiator for a sulfonation reaction, and finally, carrying out secondary cleaning on the microspheres after the sulfonation reaction is finished, so as to obtain the hydrogel. The sulfonated gelatin microspheres are obtained. According to the preparation method, the cross-linked microspheres are firstly prepared, then the cross-linked microspheres are subjected to sulfonation treatment, the reaction conditions are mild, the process is simple, amplified production is easier to perform, the microspheres (45 [mu] m) with very small particle sizes can be obtained, and the adhesion condition is avoided.

The application discloses a kind of degradable microspheres with controllable and preparation method thereof, belong to the technical field of biomedical materials.The base material of the microspheres is methacrylated gelatin, and the degradation time of the microspheres is controlled by regulating the degree of substitution of methacrylated gelatin.The microspheres are prepared by microfluidic and photopolymerization technology, and have uniform size with particle size of 10 μm to 1000 μm.The methacrylated gelatin microspheres with controllable degradation provided by the application can be used for interventional recanalization embolization therapy of substantial organ hemorrhage, realize the time sequence regulation of "hemostasis-degradation-recanalization", and have good clinical transformation value.

The invention discloses an antibacterial gelatin microsphere with a photodynamic effect and a preparation method and application of the antibacterial gelatin microsphere, and belongs to the technical field of biomedical materials and hemostasis. An olefinic unsaturated carboxylic acid monomer is grafted to a gelatin side chain through amidation reaction to obtain modified gelatin; the modified gelatin and a cross-linking agent free radical are subjected to a copolymerization reaction to form carboxyl functionalized gelatin microspheres, and a photosensitizer is covalently immobilized on the surfaces of the carboxyl functionalized gelatin microspheres through an esterification reaction to prepare the antibacterial gelatin microspheres with the photodynamic effect. According to the antibacterial gelatin microspheres with the photodynamic effect and the preparation method and application of the antibacterial gelatin microspheres, the efficiency and stability of subsequent photosensitizer loading can be remarkably improved, the postoperative infection risk is effectively solved, and the limitation that a traditional hemostatic material only pays attention to physical plugging is broken through.

The invention provides a simulated thrombus and a preparation method thereof. The preparation method comprises the following steps: preparing a polymer matrix solution; preparing gelatin microspheres and composite particles, wherein the composite particles are iron oxide particles coated with polydopamine on the surfaces; mixing the gelatin microspheres and the composite particles with the polymer matrix solution to form a composite dispersion liquid; performing network forming treatment and crosslinking treatment on the composite dispersion liquid to obtain a thrombus precursor; soaking the thrombus precursor in a PBS (Phosphate Buffer Solution) to balance the water content; and performing cyclic compression treatment on the thrombus precursor to obtain the simulated thrombus.

The present disclosure relates to the technical field of biomaterials, and provides a catechol-functionalized gelatin microsphere and a use thereof in preparation of a product for inhibiting formation of subcutaneous fluid accumulation and promoting tissue repair. The catechol-functionalized gelatin microsphere designed and prepared in the present disclosure not only has wet tissue adhesion capability, but also has good liquid absorption performance and biodegradability. The results of cell experiments show that abundant interfaces of catechol-functionalized gelatin microspheres can promote cell adhesion and proliferation, and the loose structure of the microspheres can also promote cell migration. The results of chest wall defect repair experiments of New Zealand white rabbits show that the catechol-functionalized gelatin microspheres can not only inhibit formation of subcutaneous fluid accumulation, but also recruit autologous cells in situ and promote proliferation thereof and secretion of extracellular matrix (such as collagen). These results indicate that the catechol-functionalized gelatin microsphere material has clinical application potential of inhibiting the formation of subcutaneous fluid accumulation, and promoting subcutaneous tissue regeneration and wound healing.

The invention discloses a PDA (at) Gel (at) GO nano-microcapsule for targeting colitis as well as a preparation method and application of the PDA (at) Gel (at) GO nano-microcapsule, and particularly relates to research on relieving colitis on the basis of a galactosylated carboxymethyl chitosan nano-micelle oral micro-capsule delivery drug system. According to the preparation method, a Gal-N-CMCS / Oridonin (GO) nano-micelle formed by loading Oridonin in a self-assembly manner by taking Gal-N-CMCS as a framework is constructed, then the Gal-N-CMCS / Oridonin (GO) nano-micelle is embedded into gelatin microspheres, and the gelatin microspheres are coated with dopamine to prepare the PDA / Gel / GO nano-microcapsule. Oral delivery and colon targeted release of drugs can be achieved, by inhibiting NLRP3 inflammasome activation and promoting epithelial cell regeneration, DSS-induced colitis and mucosal lesion are effectively relieved, and a new strategy and mechanism basis are provided for oral precise treatment of colitis.

The present application relates to the field of biological materials, in particular to a preparation method and application of GMS@EVs microspheres loaded with SNAP-VHH fusion protein. The present application constructs a gelatin microsphere delivery system (GMS@EVs) with capture and slow-release functions. The system specifically and efficiently captures CD9-positive exosomes secreted by mesenchymal stem cells through the stable fixation of SNAP-VHH fusion protein on the surface of gelatin microspheres, and realizes the efficient extraction of CD9-positive exosomes from the culture supernatant of mesenchymal stem cells. The present application provides specific reaction sites for the fixation of SNAP-VHH fusion protein by grafting O6-[4-(aminomethyl)benzyl]guanine on the gelatin microspheres, so that the SNAP-VHH fusion protein is stably loaded on the gelatin microspheres. Through experiments, the GMS@EVs microspheres prepared by the present application have controllable slow-release properties for exosomes, and still maintain significant fluorescence signals 48 hours after subcutaneous injection in nude mice, which is at least 16 times longer than the slow-release time of free exosomes (30 minutes of rapid diffusion), realizing the sustained release of exosomes in the local wound and enhancing the durability of the therapeutic effect.

The invention discloses a thalassemia treatment method based on a stem cell technology and application. The thalassemia treatment method comprises the following steps: collecting a peripheral blood sample of a patient, and carrying out stem cell isolated culture; constructing a nucleic acid aptamer by combining a vascular cell adhesion molecule-1 and a stromal cell-derived factor-1 in a bone marrow microenvironment; connecting a nucleic acid aptamer, a cell adhesion promoting peptide and a stem cell growth factor, and constructing a homing guiding reagent; preparing nano liposome and nano polymer particles; the nucleic acid aptamer is connected to the surfaces of the nano-liposome and the nano-polymer particles, and is grafted with a temperature-responsive material to obtain the nano-carrier with a responsive release function; stem cell growth factors and platelet-derived growth factors are mixed with gelatin microspheres to form a slow release system; the cultured stem cells are mixed with a homing guide reagent, a nano-carrier and a slow release system, and are transplanted into the body of a patient through intramedullary injection. The stem cell homing efficiency can be improved, and the treatment effect on thalassemia is improved.

The invention discloses a composite thermosensitive hydrogel embolism material and a preparation method thereof, and belongs to the technical field of biological medicines. The invention aims to solve the problems of insufficient strength, uncontrollable gel forming and development deficiency of the embolism material. The cleaning agent is composed of the following components in percentage by weight: 5 wt% of sodium alginate; 20 wt% of a developer; 8-15 wt% of a crosslinking triggering agent gelatin microsphere; 1.5 to 2 wt% of a thixotropic agent; and the balance of sterilized water for injection. The molar ratio of Ca to G is 0.3 to 0.35. At room temperature, lithium magnesium silicate forms a physical gel network, so that the material is endowed with a shear thinning characteristic, and the material can be smoothly injected through a microcatheter and stably suspends a developing agent; when the gelatin microspheres are injected into in-vivo target blood vessels at 37 DEG C, the gelatin microspheres generate thermal response phase change, released calcium ions trigger sodium alginate to generate irreversible ionic crosslinking, a high-strength chemical gel network is formed, and the gelatin microspheres are suitable for treatment of vascular diseases such as liver cancer interventional embolism and the like.

The application belongs to the technical field of three-dimensional culture and delivery of cells, and discloses a gelatin / hyaluronic acid porous hydrogel composed of a biomimetic extracellular matrix for three-dimensional culture and delivery of dendritic cells and a preparation method thereof. The method uses a semi-interpenetrating polymer network composed of methacrylic anhydride modified gelatin (GelMA) and oxidized hyaluronic acid (OHA) as a matrix, introduces temperature-responsive gelatin microspheres and dendritic cells at the same time, and then constructs a three-dimensional supramolecular hydrogel cell in-vitro culture model with decoupled pore size and stiffness after the gelatin microspheres are melted by increasing the temperature. The supramolecular hydrogel has the advantages of convenient use, simple operation, biological safety and the like, and can be used in the fields of three-dimensional culture, delivery and tumor immunotherapy of dendritic cells.

The invention discloses a multifunctional gelatin microsphere and a preparation method and application thereof, the multifunctional gelatin microsphere is prepared from gelatin, tea hair and an oxidation initiator as raw materials, and the multifunctional gelatin microsphere has multiple biological functions of water absorption, oxidation resistance, hemostasis, photo-thermal and the like. The preparation method comprises the following steps: filling an injector with a water-phase prefabricated liquid containing gelatin and tea hair, delivering the prefabricated liquid into an oil phase by a syringe needle through vertical reciprocating motion, cutting the prefabricated liquid into micro-droplets with uniform sizes through shearing force of a liquid-liquid interface and tension of an oil / water interface when the syringe needle leaves the oil phase, and solidifying the micro-droplets in the oil phase, so as to obtain the gelatin / tea hair-containing micro-droplets. And initiating crosslinking of the micro-droplets through an oxidation initiator to form the gelatin microspheres. The diameter of the microsphere is controlled by adjusting the injection rate of the prefabricated liquid, the reciprocating motion frequency of the needle and the size of the needle, the preparation process is simple, the diameter is controllable, and the cost is low. The multifunctional gelatin microspheres can be used in the biomedical fields of hemostasis, prevention of postoperative adhesion, promotion of tissue repair and the like.

The invention relates to the field of biological medicine, and discloses a medicine suspension composition for improving osteoarticular diseases and a preparation method thereof. In the embodiment 1, the composition comprises sterile normal saline and umbilical cord mesenchymal stem cell-loaded modified gelatin microspheres with the volume fraction of 50%; the microspheres are prepared from phenylboronic acid-sulfobetaine bifunctional modified gelatin, 0.2 M trehalose, a photoinitiator and stem cells through a microfluidic technology. The modified gelatin is grafted with 3-carboxyphenylboronic acid through an amido bond, and is grafted with a sulfydryl-containing sulphobetaine derivative through Michael addition; the cartilage anchoring and active oxygen removal are realized by utilizing phenylboronic acid groups, agglomeration is prevented by utilizing a sulphobetaine hydration layer, cells are protected by matching with trehalose, and the cartilage hydrogel has high dispersity, high survival rate and excellent tissue adhesion.

The invention provides an adsorption type calcium phosphate composite material with a porous structure and a preparation method thereof, and belongs to the technical field of biomedical materials. The hydrogel comprises the following components: hydroxyapatite, gelatin microspheres, poloxamer 127, a composite solution and a cross-linking agent. Wherein hydroxyapatite is used as a base material, gelatin microspheres and poloxamer 127 are added to construct a multi-stage porous structure, after freeze drying and baking for template removal, the base material is soaked in a composite solution containing chitosan, sodium alginate and RGD bioactive peptide, and then cross-linking, washing and sterilization are performed to obtain a finished product. Hydroxyapatite is homologous with human body components, has good biocompatibility and is degradable; the constructed multi-stage porous structure gives consideration to both the adsorption capacity and the substance transmission efficiency; after the base material is soaked in the composite solution, the target substance is adsorbed by the introduced amino group and carboxyl group, and the composite RGD peptide can promote cell adhesion and proliferation. The problems that an existing adsorption type calcium phosphate composite material is single in pore structure, poor in adsorption selectivity and insufficient in biological activity are solved.

The invention relates to the technical field of medical materials, and discloses hydrogel for promoting healing of diabetic wounds and a preparation method of the hydrogel. The preparation method comprises the following steps: S1, grafting 3-aminophenylboronic acid to a hyaluronic acid molecular chain to prepare a first cross-linked network precursor which is a dynamic cross-linked network, the network form of the dynamic cross-linked network can be regulated and controlled through pH, and the first cross-linked network is easy to degrade under an acidic condition and is cross-linked and cured under an alkaline condition; s2, methacrylic anhydride reacts with hyaluronic acid to form a second cross-linked network precursor; s3, loading a drug on the gelatin microspheres; s4, under the condition that the pH is 7.5-9.0, the first cross-linked network precursor and sericin form a dynamic borate bond, so that a first cross-linked network is obtained through cross-linking, under the conditions of ultraviolet irradiation and a photoinitiator, the second cross-linked network precursor is cross-linked into a second cross-linked network, the first cross-linked network and the first cross-linked network are mutually interspersed, and a dynamic borate bond is formed; and forming a hydrogel network together.

The invention relates to a preparation method of porous metal element doped hydroxyapatite microspheres as well as a product and application of the porous metal element doped hydroxyapatite microspheres. The preparation method comprises the following steps: firstly preparing beta-TCP uniformly doped with metal elements, mixing the beta-TCP with calcium carbonate powder to serve as reaction precursor powder, stirring a gelatin solution for dispersing the precursor powder and an oil phase solution at a high speed to form a water-in-oil emulsion, and curing at a low temperature to form gelatin microspheres; then, in the high-temperature calcination process of 1300-1400 DEG C, the gelatin template is decomposed, meanwhile, a precursor in the gelatin template is subjected to a solid-phase reaction, and the porous metal element doped hydroxyapatite microspheres are obtained. The microspheres with single particle size range distribution are prepared by adopting an emulsion method, then the high-temperature treatment step synchronously realizes microsphere shaping, pore generation and crystal phase conversion, and the process is simple and stable. The porous metal-doped hydroxyapatite microspheres have high specific surface area and stable mechanical strength, and the material performance can be directionally regulated and controlled by changing the types of doped metals, so that the requirements in different fields are met.

The present application relates to the field of aquaculture technology, and discloses a microbial preparation for conditioning water environment of aquaculture and a preparation process thereof. The present application prepares a microbial preparation with a capsule structure, which comprises the following raw materials: gelatin microspheres coated with mixed bacterial powder, sodium thiosulfate, ferric sulfate and nano titanium dioxide. When the microbial preparation is put into water, the gelatin is hydrolyzed, and the released sodium thiosulfate and ferric sulfate quickly decompose nitrite in the water through reduction. After a period of time, the gelatin shell of the gelatin microspheres is hydrolyzed, and the mixed bacterial powder is released. At this time, the sodium thiosulfate and ferric sulfate in the water have fully reacted, the content of nitrite in the water is low, and the water quality is good, thus providing a good environment for the initial growth of microorganisms and avoiding the problem that microorganisms will die in large quantities when directly put into water with a high content of nitrite. The nano titanium dioxide has a photocatalytic effect, and appropriate addition can help purify the water.

The invention discloses a lycium barbarum polysaccharide spray as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The lycium barbarum polysaccharide spray comprises a partially oxidized dialdehyde polysaccharide aqueous solution and lycium barbarum polysaccharide-gelatin microspheres dispersed in the partially oxidized dialdehyde polysaccharide aqueous solution, the lycium barbarum polysaccharide-gelatin microspheres take cross-linked gelatin as a matrix, and lycium barbarum polysaccharide is uniformly embedded in the cross-linked gelatin. The preparation method of the lycium barbarum polysaccharide spray comprises the following steps: preparing lycium barbarum polysaccharide-gelatin microspheres by adopting an emulsion template method, and dispersing the lycium barbarum polysaccharide-gelatin microspheres in a partially oxidized dialdehyde polysaccharide solution, thereby obtaining the lycium barbarum polysaccharide spray. The invention also discloses an application of the lycium barbarum polysaccharide spray in preparation of a medicine for preventing or / and oral cavity tissue injury. The traditional Chinese medicine active ingredient lycium barbarum polysaccharide is applied to the field of oral medicine through a modern medicine technology, and a brand new scheme with remarkable clinical value is provided for preventing and treating oral soft tissue injury, particularly tobacco-caused injury.

The application discloses a bone repair composite material with gelatin microspheres capable of releasing drugs and a preparation method thereof, and comprises the following steps: preparing a microfluid channel by 3D printing and performing surface modification on the microfluid channel by adopting tetramethyldisiloxane; adding gelatin into a phosphate buffer solution, dissolving, then adding antibiotics, stirring uniformly, and obtaining an aqueous phase; uniformly mixing mineral oil and a dispersing agent to obtain an oil phase; simultaneously feeding the aqueous phase and the oil phase into the modified microfluid channel to obtain drug-loaded gelatin microspheres; adding a crosslinking agent into the drug-loaded gelatin microspheres, freeze-drying, obtaining a drug-encapsulated gelatin drug packaging micro-particle carrier, and then mixing the drug-encapsulated gelatin drug packaging micro-particle carrier with calcium phosphate cement, and solidifying.

The invention discloses a self-gelation temperature-sensitive gelatin embolization microsphere and a preparation method and application thereof.The gelatin microsphere is crosslinked through tannic acid to form a unique structure with the surface highly crosslinked and the interior lightly crosslinked, so that the gelatin microsphere can be independently dispersed at the room temperature after being rehydrated and can be mutually fused to be self-gelated after being delivered into the body, and the self-gelation temperature-sensitive gelatin embolization microsphere has the advantages that the self-gelation temperature-sensitive gelatin embolization microsphere is formed; therefore, the original gaps among the microspheres are filled, and the embolism effect is improved. Meanwhile, the temperature-sensitive gelatin microspheres have densified surfaces and can encapsulate contrast agents or drugs in advance, so that the effect of long-acting development or drug sustained release is achieved. In addition, the gel formed after fusion of the temperature-sensitive gelatin microspheres has tissue adhesion, can effectively avoid displacement of the gel, eliminates gaps between the microspheres and the blood vessel wall, and further improves the embolism effect. The temperature-sensitive gelatin microspheres can be applied to interventional embolism treatment in the fields of tumors, vascular deformity, arthralgia and the like.

The invention relates to the technical field of biological medicine, in particular to apoptosis vesicles with a mitochondrial adjusting function, a composite material and application of the apoptosis vesicles and the composite material to preparation of medicine for promoting tendon injury repair, the apoptosis vesicles are derived from tendon stem cells, tendon stem cell apoptosis is induced through tendon stem cell apoptosis induction liquid, and the apoptosis vesicles are derived from the tendon stem cells. The apoptosis vesicles can recover macrophage mitochondrial dysfunction under inflammation conditions, reduce active oxygen in cells and mitochondria, recover mitochondrial membrane potential, regulate mitochondrial function homeostasis, inhibit the macrophage from being converted into pro-inflammatory macrophage M1 type, inhibit the apoptosis of the macrophage, inhibit the apoptosis of the macrophage, inhibit the apoptosis of the macrophage, and inhibit the apoptosis of the macrophage. The transformation to the anti-inflammatory macrophage M2 type is promoted. The invention also prepares the apoptosis vesicle composite material derived from the tendon stem cells loaded on the porous gelatin microspheres, and the composite material can inhibit achilles tendon injury induced by type I collagenase and is used for preparing medicines for promoting tendon injury repair.

The present application relates to the field of hydrogel materials, in particular to a hydrogel dressing and its preparation method and application. The present application loads deferoxamine mesylate on the gelatin microsphere material constructed by gelatin and genipin, which not only helps the dispersion of deferoxamine mesylate, but also the added gelatin can contact and degrade with matrix metalloproteinase-9 in the microenvironment of diabetic foot ulcer to induce the release of deferoxamine mesylate. And the present application forms a first crosslinking network by modifying chitosan with 3-carboxyphenylboronic acid and oxidized hyaluronic acid, and the first crosslinking network coats the gelatin microsphere material loaded with deferoxamine mesylate and epigallocatechin gallate, so that the hydrogel dressing realizes the controlled release of deferoxamine mesylate and epigallocatechin gallate, to solve the technical problem of uncontrollable release of deferoxamine mesylate.

The invention discloses a multifunctional bone repair 3D printing composite material and a preparation method and application thereof, and belongs to the technical field of bone repair material preparation. The multifunctional bone repair 3D printing composite material is prepared from the following raw materials: a degradable polymer, a compatilizer, fishbone hydroxyapatite and SrO-NaF loaded fishbone gelatin microspheres. The multifunctional bone repair 3D printing composite material has good mechanical performance, biocompatibility, mineralization ability and antibacterial performance, can be used as a bone repair material, is prepared into a multifunctional bone repair 3D printing wire through extrusion molding and then is used for preparing a bone repair product through 3D printing, and a novel material solution is provided for bone tissue regeneration and repair.