746 results about "Ergothioneine" patented technology

The invention provides recombinant escherichia coli with high yield of ergothioneine and a method for preparing ergothioneine, the recombinant escherichia coli co-expresses ergothioneine anabolic enzymes Egt1 and Egt2, the methyltransferase structural domain of the Egt1 is derived from trichoderma reesei, mycobacterium smegmatis EgtD or rhizopus nigricans, and the methyltransferase structural domain of the Egt2 is derived from the trichoderma reesei, the mycobacterium smegmatis EgtD or the rhizopus nigricans. The histidine sulfoxide synthase structural domain is derived from trichoderma reesei, schizosaccharomyces pombe or rhizopus nigricans, and the Egt2 is derived from claviceps purpurea, neurospora crassa or aspergillus niger. After the recombinant escherichia coli obtained by screening is cultured for 68 hours in a 5L fermentation tank, the yield of ergothioneine can reach 5g / L or above; wherein after recombinant escherichia coli co-expressing Egt1 enzyme (the structural domain of methyltransferase is derived from mycobacterium smegmatis EgtD, and the structural domain of histidine sulfoxide synthase is derived from trichoderma reesei) and Egt2 enzyme (derived from neurospora crassa) are cultured in a 5L fermentation tank for 68 hours, the yield of ergothioneine can reach 8.7 g / L, and the production intensity reaches 127.9 mg / (L.h).

The invention discloses an anti-aging composition containing ergothioneine, glutathione and fish collagen, relates to the technical field of anti-aging compositions, and aims to solve the technical problems that a traditional anti-aging composition lacks attention to the aspects of cell energy metabolism, barrier repair and the like, and the overall skin improvement effect is not obvious. The ergothioneine is used as a natural antioxidant, and the content of the ergothioneine is 0.01-5wt%; the ergothioneine participates in a synergistic effect of an intracellular antioxidant enzyme system, so that tyrosinase activity is effectively inhibited, melanogenesis is reduced, and DNA and protein are protected from oxidation damage; in a simulated strong ultraviolet environment, compared with skin cells not using the ergothioneine-containing product, the content of free radicals in the cells is reduced by 30% after the ergothioneine-containing product is used; the ergothioneine can also regulate a cell signal channel, promote cell metabolism and maintain normal growth of cells. The mask has the advantages of accelerating skin metabolism and improving the self-repairing ability of the skin.

The invention discloses a hydroxytyrosol-ergothioneine eutectic crystal as well as a preparation method and application thereof, and belongs to the technical fields of medicines, foods, daily chemicals and the like. The invention provides a hydroxytyrosol-ergothioneine eutectic crystal. The X-ray powder diffraction pattern of the hydroxytyrosol-ergothioneine eutectic crystal has characteristic diffraction peaks at least corresponding to the positions where the 2 theta value is 4.21 degrees + / -0.2 degrees, 8.36 degrees + / -0.2 degrees, 12.29 degrees + / -0.2 degrees, 15.29 degrees + / -0.2 degrees, 19.40 degrees + / -0.2 degrees, 20.98 degrees + / -0.2 degrees, 23.12 degrees + / -0.2 degrees and 28.73 degrees + / -0.2 degrees. According to the hydroxytyrosol-ergothioneine eutectic, the melting point of hydroxytyrosol is increased, the hygroscopicity of hydroxytyrosol is remarkably reduced, and meanwhile the stability of hydroxytyrosol and ergothioneine is enhanced. In addition, after the ergothioneine hydroxytyrosol forms the eutectic crystal, the ergothioneine hydroxytyrosol can also play better anti-oxidation and anti-aging effects.

The present invention provides methods, compositions (e.g., eyedrop or spongarion composition), and uses for alleviating and preventing age-associated vitreous degeneration in a mammal, comprising administrating to the mammal in need thereof a therapeutically effective amount of L-ergothioneine, an analog or derivative thereof, or a pharmaceutically acceptable salt, acid, ester, polymer, analog or derivative thereof, as an active ingredient. In particular, L-ergothioneine is capable of scavenging chronic reactive oxygen species directly and radically to mitigate and prevent HA depolymerization and HA decreasing-viscosity in the mammal's vitreous body; and / or improving vitreous liquefaction in the mammal.

Cosmetic composition, e.g. an anti-ageing composition, comprising retinyl palmitate (pref. 0.25-0.5 wt.%), Silybum marianum seed oil (pref. 1-3 per cent by weight), betaine (pref. 2-4 weight per cent)

The invention discloses a method for simultaneously detecting ergothioneine, ergosterol, ergosterol peroxide and lentinine in lentinus edodes, and belongs to the technical field of measurement of active ingredients of the lentinus edodes. The method comprises the following steps: detecting a shiitake mushroom extracting solution by adopting an ultra-high performance liquid chromatography-tandem mass spectrometry mode, wherein a chromatographic column is an HILIC or C18 chromatographic column; the mobile phase A and the mobile phase B are an aqueous solution containing formic acid and a methanol or acetonitrile solution containing formic acid respectively; the mass spectrum conditions comprise an electrospray ionization source; a positive ion multiple reaction monitoring mode; the ion spraying temperature is 315-325 DEG C; the temperature of the ion transmission tube is 320-330 DEG C; and the ion spraying voltage is 3500 V to 3700 V. The method has the characteristics of high sensitivity, high selectivity and high accuracy, and is suitable for quantitative detection of ergothioneine, lentinine, ergosterol peroxide and ergosterol compounds in shiitake mushrooms.

The invention provides a strain for producing ergothioneine, and belongs to the technical field of synthetic biology. The strain expresses and encodes a gene of EgtD methyltransferase, and the methyltransferase comprises an amino acid sequence as shown in SEQ ID NO. 15. According to the application, an efficient escherichia coli expression system is adopted, a plurality of EgtD gene sequences are screened from a database for in-vitro enzyme activity characterization by taking currently reported EgtD enzyme from Mycobacterium smegmatis with the highest activity as a contrast, and finally, an EgtD strain of which the activity is improved by 71% is successfully screened. The high-activity EgtD is a methyltransferase gene derived from Gammaproteobacteria bacterium, the enzyme activity is improved by 71% compared with that of a control group, and the yield of an engineering strain ergothioneine containing the enzyme is improved by 30%.

The invention belongs to the technical field of health-care food, and provides a composition containing ergothioneine and having sleep-aiding, whitening, anti-aging and anti-inflammatory effects and application thereof. The ergothioneine composition disclosed by the invention is prepared from ergothioneine, collagen peptide, carnosine, nicotinamide, an olive leaf extract and a chamomile extract. The components in the composition are matched for use, the synergistic effect is obvious, the whitening, anti-aging and anti-inflammatory effects are obviously improved, meanwhile, the composition also has a sleep-aiding effect, and the comprehensive performance is excellent.

The invention relates to the technical field of cosmetics, and particularly discloses a composition with after-sun repairing and whitening effects and a preparation method thereof. According to the present invention, various efficient components such as the ergothioneine compound, the acetylchitosamine, the nicotinamide, the sodium hyaluronate, the inositol, the aloe vera extract, the glycerophosphoinositide choline salt, the tetrahydromethylpyrimidine carboxylic acid, the oat beta-glucan compound, the citrus peel extract compound and the skin brightening agent are scientifically matched; according to the present invention, the excellent after-sun repairing and whitening effect is achieved, the comprehensive after-sun skin care is achieved, the skin state can be improved, and the skin health degree can be improved. The application creatively finds that when the glycerophosphoinositide choline salt, the citrus peel extract compound and the skin brightening agent are compounded, the effects of the composition in the aspects of after-sun repairing and whitening can be improved at the same time, and the comprehensive effect is relatively good.

The invention relates to an anti-oxidation composition based on a reverse micelle synergistic technology and a preparation method and application thereof, the anti-oxidation composition comprises a reverse micelle structure and an aqueous solution containing an anti-oxidation active matter, and the reverse micelle structure wraps the aqueous solution containing the anti-oxidation active matter. The reverse micelle structure is used for wrapping the water-soluble antioxidant active matter, oxidation or degradation discoloration of the water-soluble antioxidant active matter is prevented, after the antioxidant active matter such as hydroxytyrosol, ergothioneine and dipotassium glycyrrhizinate is wrapped by the reverse micelle, the stability and the effect are remarkably improved, and after the reverse micelle wrapping, the effect of the antioxidant active matter is improved by 17% or above compared with an aqueous solution before wrapping.

The invention discloses an ovarian protection composition containing ergothioneine and a preparation method of the ovarian protection composition, and relates to the technical field of ovarian protection compositions. The ovary protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.1 to 0.5 part of the ergothioneine, 0.05 to 1 part of S-equol, 1 to 5 parts of reducing coenzyme Q10, 1 to 3 parts of vitamin E, 10 to 20 parts of chitosan oligosaccharide, 30 to 50 parts of microcrystalline cellulose, 3 to 8 parts of hydroxypropyl methyl cellulose, 0.5 to 2 parts of silicon dioxide and 0.5 to 2 parts of a stabilizer. By adopting the stabilizer, photooxidation and hydrolysis of sensitive components are inhibited through molecular action, and the long-term stability of the composition in a humid and hot environment is remarkably improved in combination with the physical barrier action of silicon dioxide. Through the antioxidant synergistic effect of the ergothioneine and the vitamin E, the hormone regulation effect of the S-equol and the energy metabolism improvement effect of the coenzyme Q10, a multi-layer ovarian function protection system is constructed, and the health state of ovaries is improved. Through a special process, the tablet can be rapidly disintegrated and slowly released in an intestinal targeted manner, burst release of active ingredients is avoided, and the absorption efficiency is improved.

The invention discloses a liposome vesicle for inhibiting ergothioneine degradation and a preparation method thereof, and belongs to the technical field of cosmetics. According to the ergothioneine liposome and the preparation method thereof disclosed by the invention, by constructing an embedding structure, the ergothioneine is embedded in a phospholipid bimolecular layer of the liposome vesicle, and the outer layer of the phospholipid bimolecular layer is also wrapped with a biocompatible high-molecular polymer shell, so that the ergothioneine is doubly wrapped by the phospholipid bimolecular layer and the high-molecular polymer shell of the ergothioneine liposome; and the degradation of ergothioneine is reduced. And the phospholipid bilayer base material for preparing the liposome vesicles can generate ionic interaction with trimethylamine degraded by the ergothioneine, so that the odor problem of the ergothioneine is effectively reduced.

The invention provides a method for promoting collagen production. The method comprises the step of promoting type III collagen production by using ergothioneine. The application finds that the low-concentration ergothioneine has the effect of promoting the generation of the type III collagen, and particularly, the enhanced effect of promoting the generation of the type III collagen is shown along with the reduction of the concentration of the ergothioneine, so that a new thought is provided for the application of the ergothioneine.

The invention discloses a preparation method of an oral liver protection composition containing ergothioneine, and relates to the technical field of ergothioneine application. The oral liver protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.5 to 5 parts of the ergothioneine, 10 to 30 parts of silibinin, 20 to 50 parts of a phospholipid complex, 5 to 15 parts of a liver targeting carrier, 2 to 8 parts of a disintegrating agent, 1 to 5 parts of an adhesive and 2 to 4 parts of an antioxidant. Free radicals are efficiently captured through a polycyclic conjugated pi electron system of the antioxidant, a hydrogen bond network is formed by a heteroatom bridging effect of the antioxidant, a galactose group of the liver targeting carrier and the disintegrating agent, and the stability of the system is synergistically improved; the phospholipid complex is embedded with a hydrophobic drug and a hydrophilic carrier, and the drug is accurately delivered to the liver by combining the specific recognition capability of the liver targeting carrier; the ergothioneine and the silybin form a bidirectional synergistic liver protection mechanism by removing reactive oxygen free radicals and regulating and controlling cell signal channels respectively.

The invention discloses a fusion protein which comprises a first structural domain with an EgtD catalytic function, a second structural domain with an Egt1 catalytic function and a third structural domain with an EgtE catalytic function, and the amino acid sequence of the first structural domain with the EgtD catalytic function is shown as SEQ ID NO.1; the amino acid sequence of the second structural domain with the Egt1 catalytic function is as shown in SEQ ID NO. 2; and the amino acid sequence of the third structural domain with the EgtE catalytic function is as shown in SEQ ID NO. 3. The fusion protein is subjected to heterologous expression in engineering bacteria, so that the yield of ergothioneine is remarkably increased.

The invention belongs to the technical field of cosmetics, and particularly discloses an anti-aging and repairing cosmetic composition as well as a preparation method and application thereof. The cosmetic composition is prepared from the following raw materials in parts by weight: 0.05 to 1.5 parts of an ergothioneine pine mushroom extract, 1 to 10 parts of oat firming protein, 0.01 to 1 part of Marilong eight-treasure raw powder, 0.001 to 1 part of tetrahydromethylpyrimidine carboxylic acid, 0.000001 to 0.01 part of conopeptide, 0.00001 to 0.1 part of acetyl hexapeptide-8, 0.01 to 1 part of D-panthenol, 0.01 to 3 parts of nicotinamide and 0.01 to 1 part of sodium hyaluronate. The cosmetic composition provided by the invention simultaneously has multiple effects of immediate wrinkle removal, long-term anti-aging wrinkle removal, barrier repair and the like, the anti-aging repair effect of the product is greatly improved, and the maintenance process of consumers is effectively simplified. The prepared eye cream is small in irritation and good in anti-aging and repairing effects.

The invention discloses a genetically engineered bacterium and application of the genetically engineered bacterium in preparation of ergothioneine. The genetically engineered bacterium carries genes egtB, egtD and egtE for coding ergothioneine synthetase, and the genes egtB, egtD and egtE are assembled with a protein scaffold assembly SGP; the protein scaffold assembly SGP comprises a protein scaffold GBD, a protein scaffold SH3 and a protein scaffold PDZ. The inventor finds that the ergothioneine synthetases egtB, egtD and egtE are assembled by using the protein scaffold assembly SGP, so that the ergothioneine can be efficiently synthesized, and the protein scaffold assembly has an important application prospect.

The invention discloses a composition capable of resisting inflammation, increasing blood oxygen content, inhibiting cancer cell growth and relieving tumor treatment side effects. The composition is specifically prepared from exocarpium citri grandis, ginseng, cortex cinnamomi, ergothioneine, herba houttuyniae, kelp, herba taraxaci, rhizoma curcumae longae, epigallocatechin gallate, naringin, chitosan oligosaccharide, zinc gluconate and taurine. The composition provided by the invention integrates the treatment concepts of softening hardness to dissipate stagnation in the traditional medicine and treating according to the evidence in the modern medicine, and the raw materials are scientific in compatibility, have a synergistic effect and jointly exert the effects of resisting inflammation, improving body immunity, improving the blood oxygen content level of a focus part and the like; the compound has the advantages of well inhibiting growth of cancer cells, inhibiting expression of hypoxia-inducible factors HIF, relieving side effects of tumor treatment and the like, is safe and efficient, and has a wide application prospect.

The invention discloses a bacteriophage defense system as well as a construction method and application of the bacteriophage defense system in anti-bacteriophage engineering bacteria. The bacteriophage defense system is derived from Escherichia coli Nissle 1917, and comprises proteins as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, and derived proteins of the proteins as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. According to the invention, a Type II restriction-modification system with strong bacteriophage defense capability is identified from Escherichia coli Nissle 1917, and the Type II restriction-modification system is transplanted into strains such as Escherichia coli W3110, MG1655 (DE3), BL21 (DE3) and the like, so that the strains are endowed with superstrong bacteriophage resistance. The system is successfully applied to industrial engineering bacteria for producing ectoine, fine glufosinate-ammonium, ergothioneine and other products, and the bacteriophage defense capability of the industrial engineering bacteria is effectively enhanced on the basis that the production capacity of the industrial engineering bacteria is not seriously influenced.

The embodiment of the invention discloses application of carnosine, the carnosine can be used for relieving the smell of a composition containing ergothioneine, on the basis, the invention further provides the composition containing ergothioneine and application, and the composition containing ergothioneine comprises ergothioneine, carnosine and water.

The invention discloses a Yarrowia lipolytica strain for synthesizing ergothioneine and application of the Yarrowia lipolytica strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a key enzyme TrEGT1 is subjected to semi-rational transformation, so that the ergothioneine yield of a strain expressing a mutant is increased by 240.9%, precursor metabolic network screening is further modularized, precursor flux is enhanced, a competitive approach of a precursor is knocked out and weakened, and strategies such as fermentation process optimization and the like are further optimized; and when the constructed strain is fermented in a 5L bioreactor, the yield of ergothioneine can reach 10.3 g / L. The highest-level ergothioneine synthesis reported at present is realized, the feasibility of synthesizing the ergothioneine by using the Yarrowia lipolytica as the chassis cell is verified, and a reference value is provided for efficiently synthesizing the ergothioneine by using other chassis cells.

The invention discloses a collagen microsphere ergothioneine compound, a multifunctional efficient broad-spectrum sunscreen composition containing the collagen microsphere ergothioneine compound and a preparation method, the collagen microsphere ergothioneine compound comprises III-type recombinant human collagen microspheres and ergothioneine, the mass ratio of the III-type recombinant human collagen microspheres to the ergothioneine is (0.5-2): (5-20); the compound further comprises Ectoine, and the content of the Ectoine is 15-140 times that of the III type recombinant human collagen microspheres. Through the use of the collagen microsphere ergothioneine compound, the light stability of the ergothioneine can be protected, quick and efficient permeation can be realized by carrying the ergothioneine by utilizing the extremely high permeability of the III type recombinant human collagen microspheres, and the bioavailability of the ergothioneine and the stability of the ergothioneine in a formula system of the sunscreen composition are improved; meanwhile, the sunscreen composition integrates multiple functions of high-power sunscreen, anti-inflammatory repair, blue light resistance and the like, and has very good market prospects and economic values.

The invention belongs to the technical field of nano-enzymes, and particularly relates to a CuPH (at) KMO composite nano-enzyme material as well as a preparation method and application thereof. Aiming at the problems of poor catalytic activity, complex steps, high raw material cost and low biocompatibility of nano-enzyme prepared by the existing method, the preparation method comprises the following steps: by taking terephthalic acid and histidine as dual-coordination copper-based nano-enzyme as a precursor, introducing manganese oxide in situ under a hydrothermal condition; and the flower-like nano mimic enzyme which is uniform in size and has high laccase-like catalytic characteristics at the same time is synthesized. The composite nano-enzyme provided by the invention can catalytically oxidize phenol amine organic compounds under the condition that the pH value is 3-9, can realize the detection of the content of ergothioneine in cosmetics and nutritional supplement capsules, and can be applied to the field of cosmetic and food safety monitoring.

The invention discloses a method for efficiently producing ergothioneine by metabolic engineering modified escherichia coli and application, and belongs to the technical field of biology. By adopting the recombinant escherichia coli EGT-1-B * provided by the invention, glucose can be converted into ergothioneine; therefore, the raw materials are cheap and easy to obtain, additives and heavy metals which are harmful to human health are avoided, a good foundation is laid for later product purification, and the requirements of commercial application can be met. Furthermore, genes related to metabolism of histidine, methionine, cysteine and S-adenosylmethionine are further overexpressed in the recombinant escherichia coli EGT-1-B *, so that the content of precursor amino acid synthesized by the ergothioneine is increased, and meanwhile, after the gene yqaB is knocked out, the yield of the ergothioneine is further increased; the utilization rate of glucose to ergothioneine can be further improved, and the yield of ergothioneine can reach 140.29 mg / L.

The invention discloses a composition for treating prostatitis and a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 5 to 15 parts of ergothioneine, 10 to 20 parts of cistanche total glycosides, 25 to 45 parts of phytosterol, 450 to 550 parts of pumpkin seed oil, 180 to 250 parts of fructus cannabis oil and 250 to 350 parts of auxiliary materials. Through the synergistic effect of various natural active components, the composition maintains the balance of overall metabolism of a body while reducing inflammation and inhibiting prostate cell proliferation, side effect risks possibly brought by a traditional single-component treatment scheme are avoided, and the composition has higher clinical application potential. The composition disclosed by the invention has a wide market application prospect and popularization value by virtue of good safety and compliance.

The embodiment of the invention discloses a color-stable whitening composition and a skin whitening product. The color-stable whitening composition comprises arbutin, tranexamic acid and a color stabilizer, and the color stabilizer comprises ascorbyl glucoside and ergothioneine. The whitening composition is stable in color and not prone to discoloring, and meanwhile the good skin whitening and anti-oxidation effects can be achieved.

The invention belongs to the technical field of medicines, and particularly relates to a multi-effect composition containing ergothioneine, a preparation method of the multi-effect composition and application of the multi-effect composition in eye products. The multi-effect composition is prepared from ergothioneine, tromethamine and sodium hyaluronate according to the mass ratio of (0.5 to 10) to (1 to 5) to (0.5 to 3). According to the multi-effect composition containing the ergothioneine, the tromethamine in the composition can protect the stability of the ergothioneine under the conditions of illumination and high temperature; the ergosulfide can protect the activity of macromolecular sodium hyaluronate. The synergistic compatibility of the ergothioneine, the sodium hyaluronate and the tromethamine is utilized, so that the moisturizing effect and the eye protection effect of the composition can be remarkably improved. The ergothioneine-containing multi-effect composition provided by the invention has the effect of preventing cataract through specific components and proportions, and has the advantages of long-acting eye moistening and good stability.

The invention discloses an oral joint protection composition containing ergothioneine and a preparation method thereof, and relates to the technical field of joint protection compositions. The oral joint protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.05 to 5 parts of the ergothioneine, 20 to 50 parts of glucosamine salt, 10 to 30 parts of chondroitin sulfate, 2 to 10 parts of curcumin, 30 to 70 parts of a diluent, 1 to 10 parts of an adhesive, 0.5 to 5 parts of a lubricant, 2 to 10 parts of a disintegrating agent and 0.1 to 0.5 part of an antioxidant stabilizer. Through synergistic compatibility of active ingredients such as ergothioneine, curcumin and the like, a multi-dimensional action network of oxidation resistance, inflammation resistance and cartilage metabolism regulation is formed, the inhibition effect on joint inflammation is remarkably improved, and the limitation of a single ingredient is broken through.

The invention relates to the field of cosmetics, in particular to the field of A61K8, in particular to a multi-effect skin-care liposome whitening composition and a preparation method thereof. The multi-effect skin-care lipidosome whitening composition is prepared from the following raw materials: a whitening functional component, polyhydric alcohols, an emulsifier, a plant extract, lipidoids and deionized water, wherein the whitening functional component is prepared from glabridin, tranexamic acid, nicotinamide, acetylchitosamine, diglucosyl gallic acid, ectoin and ergothioneine. The multiple-effect skin-care liposome whitening composition has the effects of inhibiting the activity of tyrosinase, improving the DPPH clearance rate, inhibiting hyaluronidase and the like, and it is guaranteed that the product has excellent whitening and skin-care effects.

The invention discloses an ergothioneine composition with an anti-aging function, and relates to the field of health-care foods, and the composition has the effects of resisting aging and cell molecule oxidation reaction. The composition disclosed by the invention is prepared from the following composition components: ergothioneine (0.162 to 0.185), a wheat germ extract (0.063 to 0.085), coenzyme Q10 (0.042 to 0.063), quercetin (0.35 to 0.55), a turmeric extract (0.032 to 0.054), a black pepper extract (0.003 to 0.006) and aminobutyric acid (0.205 to 0.227). The composition is used for assisting body functions in eliminating lipid, protein, DNA (deoxyribonucleic acid) and the like in cells damaged by oxidative stress in a body. The composition is prepared into equivalent particles by fully and physically stirring and uniformly mixing, is convenient for human body to absorb, and can remarkably improve the antioxidant capacity and delay the aging process after being taken for a long time. The composition not only can effectively resist oxidative stress disoperation, but also can promote cell repair. The raw materials of the composition can be purchased in the market, and the composition is high in production benefit and beneficial to wide application.