72 results about "Ergothioneine" patented technology

The present application relates to a preservative-free anti-aging soothing composition containing high mountain plant extract and multiple fermentation filtrate, belonging to the technical field of cosmetics. The composition comprises high mountain plant extract complex, multiple fermentation filtrate, anti-aging active and vegetable oil. The organic acids in the multiple fermentation filtrate form a natural buffer system, maintaining a weakly acidic environment to inhibit microbial growth; at the same time, the natural components in the fermentation filtrate provide protection for nicotinamide mononucleotide and ergothioneine, delaying their degradation. The present application does not need to add additional preservatives, and has the functions of anti-aging, soothing and self-preserving.

The application relates to a method for extracting ergothioneine and selenoprotein from spirulina, wherein after culturing FACHB-Se1500 strains under the above-mentioned nitrogen deficiency induction condition, a product is obtained, and after purification, selenoprotein containing ergothioneine is obtained; the FACHB-Se1500 strains are cultured in a Zarrouk+Se culture medium for 10 days, and the strain culture conditions are as follows: 25-30 DEG C, pH=9.5, and light intensity is 40 muE.m-2.s-1 ‑2 ·S ‑1 After reaching the plateau, the strains are respectively transferred to a Zarrouk-N, a Zarrouk-Fe and a Zarrouk-Mg culture medium for induction culture for 2 days, and after collecting the algal powder, selenoprotein containing ergothioneine is obtained after purification. The application further screens a spirulina mutant strain with high selenium tolerance, wherein the algal strain tolerating 800 mg / L Na2SeO3 has the best salt tolerance and can tolerate 1000 mM NaCl; and it is found again through the nitrogen deficiency induction that the ergothioneine content can reach 103.65 mu g / g.

The application discloses a functional composition for improving bone and joint health, a conditioner and application. The functional composition comprises a first active component and a second active component; the first active component comprises elastin peptide, haematococcus pluvialis and ergothioneine; and the second active component comprises one or more of houttuynia cordata and non-denatured type II collagen. The application realizes the protection effect on joint, cartilage and bone health by scientific matching of the components and synergistically improving the glycosaminoglycan level in chondrocytes and the osteocalcin level in osteoblasts. Meanwhile, the components in the functional composition for improving bone and joint health are natural and have good biocompatibility, and have no obvious side effects, so that the application provides a kind of drug or a substitute for the treatment and prevention of bone diseases.

The present invention discloses a β-carotene-grape seed extract eye-care preparation for retinal protection, relating to functional health foods. The preparation comprises active components and food-acceptable excipients. In parts by weight, the active components include 1–4 parts lutein, 0.5 –2 parts meso-zeaxanthin, 2 – 6 parts β-carotene, 8 – 25 parts blueberry extract, 5 – 18 parts grape seed extract, 0.2–1 part L-ergothioneine, 0.5–3 parts saffron extract, and 1–5 parts phosphatidylserine. The excipients comprise a filler, disintegrant, binder, and lubricant, with a weight ratio of active components to excipients of 1:1 to 1:4. By combining these eight components at specific ratios, the invention achieves synergistic effects, protects retinal health, and reduces blue-light damage. Dual-phase microencapsulation based on solubility, together with standardized preparation steps, improves stability and utilization efficiency.

The application belongs to the technical field of biological medicine, and particularly relates to an anti-aging ergothioneine composition for improving mitochondrial function and a preparation method thereof. The composition comprises the following components: ergothioneine, sea hao polysaccharide and entada extract. The ergothioneine has good mitochondrial targeted antioxidant activity, can up-regulate the expression of Sirt1 to activate PGC-1a, and promote mitochondrial biosynthesis; the sea hao polysaccharide is helpful to restore mitochondrial function, promote the generation of mitochondrial high-energy phosphoric compounds, improve cell activity and energy supply for regeneration, and can up-regulate the expression of key genes for mitochondrial biosynthesis and ATP synthase related genes. The entada extract can synergize with the ergothioneine and the sea hao polysaccharide, significantly down-regulate the expression of aging related proteins such as p53, p21 and p16 in cells, simultaneously inhibit the transcription of aging related genes, and has a good protective effect on aging cells.

This invention discloses a coconut oil antibacterial and deodorizing essence, its preparation method, and its application. The essence consists of: 12-50% coconut oil, 4-10% butylene glycol, 0.1-2% sodium hyaluronate, 1-5% panthenol, 2-5% lactic acid, 2-5% potassium lactate, 1.5-6% emulsifier, 0.1-0.6% 4-tert-butylcyclohexanol, 0.1-6% ectoine, 0.1-6% ergothioneine, 1-2% non-irritating preservative composition, and the remainder being pure water. The components work synergistically. Disposable sanitary products using this essence can inhibit bacteria and deodorize, soften the skin, relieve itching, improve redness, and soothe sensitivity. Washing and care products using this essence have good inhibitory effects on harmful bacteria, build a natural biological protective film on the skin surface, and have anti-inflammatory, soothing, repairing, anti-itch, and redness-reducing properties, leaving the skin moisturized and firm.

This application belongs to the field of medical dressing technology, specifically relating to dressing compositions, absorbable hemostatic dressings, and their preparation methods. This application provides a dressing composition that, by compounding modified chitosan with modified ergothioneine, forms a dressing composition with non-swelling properties upon contact with water. The chitosan in this application is esterified to introduce hydrophilic carboxyl groups, improving its solubility. Simultaneously, aspartic acid itself promotes cell proliferation and wound healing. Therefore, the dressing prepared from this composition can maintain a stable shape and volume in a body fluid environment, effectively reducing mechanical pressure on surrounding tissues, thereby effectively avoiding various complications caused by material expansion and effectively avoiding the risk of traditional hemostatic materials swelling upon contact with water and compressing nerve tissue. Furthermore, the compounding of modified ergothioneine and modified chitosan in this application not only provides non-swelling and absorbable properties upon contact with water but also exhibits high adhesion and high antioxidant properties.

The invention provides a composition. The composition comprises the following components in parts by weight: 0.001-5 parts of a PDRN-ergothioneine compound; 0.001 to 5 parts by weight of achillea millefolium extract; and 0.01 to 0.5 part by weight of a sphingomonas fermentation product extract. The invention also provides a preparation method of the composition and a sunscreen composition using the composition. According to the composition provided by the invention, the ergothioneine, the PDRN, the achillea millefolium extract and the sphingomonas fermentation product are scientifically proportioned to construct a functional system covering an ultraviolet injury full path, so that the deep synergy and synergism of all active ingredients in action time sequence, cell target and biological effect are realized. The composition provided by the invention covers a multi-channel synergistic effect system of a light damage whole chain, and the light protection effect is excellent; and the composition has a synergistic effect on the traditional sun-screening agent, and can significantly improve the sun-screening index, enhance the light stability and improve the product experience.

The present application relates to the field of animal embryo engineering technology, and provides a method for improving the development ability of bovine IVP embryos. In the production of bovine in vitro embryos, by adding PAPPA, ergothioneine and selenocysteine to the bovine oocyte in vitro maturation culture solution, early embryo culture solution, late embryo culture solution, freezing solution and thawing solution, the in vitro maturation performance of oocytes and the development performance and vitality of the subsequent obtained embryos can be effectively improved. Studies have shown that after adding the above additives, the maturation rate of oocytes, the cleavage rate, the blastocyst rate of embryos, and the freezing survival rate, the hatching rate and the ATP content of embryos after freezing preservation are significantly improved, which has important application value.

This invention discloses a bio-dress containing multiple anti-aging factors and its preparation method. The bio-dress, by weight percentage, comprises 1-10% collagen solution, 2%-15% styraxine, 0.5%-8% ergothioneine, 1%-12% ectoine, and auxiliary ingredients. This bio-dress integrates multiple anti-aging factors, and through the synergistic effect of these factors, it can effectively improve skin aging problems. It is suitable for medical aesthetics and daily skincare, possessing good anti-aging effects and skin safety.

The application provides a composition for improving mitochondrial function and application thereof, and relates to the technical field of hyaluronic acid grafts.The application first finds that hyaluronic acid oligosaccharide-cyclopentapeptide grafts obtained by covalent combination of hyaluronic acid oligosaccharides and cyclopentapeptides which do not have the effect of improving mitochondrial function alone can effectively improve mitochondria or restore mitochondrial function and cell function, thereby providing a new functional ingredient for developing mitochondrial function regulators, cell function regulators, and products for improving the ability of cells to resist aging.Further, the application also finds that the hyaluronic acid oligosaccharide-cyclopentapeptide grafts combined with ergothioneine can produce a synergistic effect, thereby bringing beneficial effects for further improving mitochondrial function, cell function, and the ability of cells to resist aging.

This invention discloses an EGCG supramolecular compound with antioxidant, liver-protective, and fat-reducing effects, its preparation method, and composition. The supramolecular compound is formed by non-covalent bonding between epigallocatechin gallate (EGCG) and ligand molecules containing trimethylamine and carboxyl groups; the supramolecular compound has a co-amorphous structure; the ligand molecules containing trimethylamine and carboxyl groups are betaine, ergothioneine, or L-carnitine. This invention can solve the problems of low stability and bioavailability of EGCG. It can significantly improve the solubility of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine, reduce the hygroscopicity of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine, and enhance the stability and bioavailability of EGCG and its ligands, including betaine, L-carnitine, and ergothioneine.

This invention provides a method for constructing a high-yield ergothionein-producing Bacillus amyloliquefaciens engineered strain and its application. The engineered strain uses the food-grade safe strain Bacillus amyloliquefaciens HZ12 as the starting strain. Through genetic engineering, a key enzyme gene for ergothionein biosynthesis is heterologously expressed in Bacillus amyloliquefaciens. An RBS co-expression strategy is employed to express the mutant gene NcEgt1 derived from Neurospora crassa. Y174F / E51A Co-expression of the EgtE gene derived from *Mycobacterium smegmatis* successfully achieved heterologous synthesis of ergothioneine in *Bacillus amyloliquefaciens*. Under these conditions, the yield of ergothioneine reached 402.68 mg / L, marking the achievement of ergothioneine production in *Bacillus amyloliquefaciens* from scratch. Subsequently, through chassis strain modification, the yield of ergothioneine was further increased to 690.43 mg / L.

This invention discloses a food composition with antioxidant and collagen synthesis-promoting effects and its preparation method, relating to the field of food technology. The food composition comprises the following ingredients in parts by weight: 300-500 mg collagen, 100-400 mg astragalus polysaccharide, 100-400 mg grape seed proanthocyanidins, 10-200 mg catechins, 5-100 mg ergothioneine, and 5-50 mg soybean oligopeptides. This food composition can increase the collagen content in the skin, enhance facial skin elasticity, reduce transepidermal water loss, and improve skin aging-related conditions. This invention constructs a dual-core synergistic formula of "antioxidant + collagen synthesis promotion" through the scientific formulation of seven active ingredients. All components of this invention use food-grade raw materials, making the formula mild and safe; all ingredients are natural extracts or edible raw materials, ensuring high safety, no obvious toxic side effects, and long-term daily use, making it suitable for a wide range of people.

The application provides a composition and application thereof in preparation of a mitochondrial function regulator, and relates to the technical field of hyaluronic acid grafts. It is found for the first time in the application that N-sodium hyaluronoyl glutamate can effectively improve or restore mitochondrial function, improve cell energy metabolism, and achieve the effects of improving cell aging and / or improving cell activity. A new efficacy component is provided for developing a mitochondrial function regulator, a product for preventing and / or improving aging; further, N-sodium hyaluronoyl glutamate can break the plateau period of the action of ergothioneine, and provides a more effective scheme for further reversing mitochondrial function.

This invention discloses an oral liquid containing astaxanthin and its preparation method, which is prepared from raw materials comprising the following weight percentages: 0.5%~2% astaxanthin burst beads, 8%~12% collagen peptides, 0.1%~0.3% sodium hyaluronate, 0.01%~2% elastin peptides, 0.01%~0.05% spermidine, 0.005%~0.02% ergothioneine, and 0.001%~0.01% pyrroloquinoline quinone. The astaxanthin burst beads are prepared using astaxanthin oil as the core material and sodium alginate and chitosan as the wall material. This invention, by using astaxanthin burst beads with higher stability and bioavailability in the oral liquid, combined with other ingredients, can improve the user's skin moisture content and elasticity index, reduce wrinkle depth, reduce skin yellowing, and better exert the effects of the oral liquid.

The application provides an eye-care composition solution for relieving visual fatigue and a preparation method and application thereof, and belongs to the technical field of eye-care products. The composition solution comprises the following raw materials in mass percentage: Psidium guajava fruit powder 0.4-4%; Aronia melanocarpa fruit powder 0.8-4%; dihydroquercetin 0.01-0.2%; ergothioneine 0.01-0.06%; and the balance is water. The application takes Psidium guajava fruit powder and Aronia melanocarpa fruit powder as the main body, and is supplemented by dihydroquercetin and ergothioneine for joint action, so that the antioxidant and anti-inflammatory capacity of the eye can be improved, and the tear secretion of dry eye mice can be increased. The composition of the application produces a synergistic effect between the plant extracts and the high-efficiency active ingredients through scientific proportioning, aims to simultaneously relieve visual fatigue and improve dry eye symptoms, and has high product development value.

A composition for reducing copper-induced liver cell death in a subject, such as a human, dog, or cat, is provided. The composition includes L-ergothioneine and chrysophanol in an amount which provides a synergistic effect in the reduction of copper-induced liver cell death. A ratio of the L-ergothioneine to chrysophanol ranges from 1:1 to 1:8. The composition can include the L-ergothioneine in an amount of 100 μM and the chrysophanol in an amount of 100 μM, for example. Alternatively, the composition can include the L-ergothioneine in an amount of 12.5 μM and the chrysophanol in an amount of 100 μM, for example. The composition can be provided in the form of a liquid, tablet, capsule, soft chew, or another form.

The present invention provides S-methyl-L-ergothioneine for use in diagnosis and / or prognosis. The invention also provides a method for the diagnosis and / or prognosis of a renal disease comprising the step of determining the amount of S-methyl-L-ergothioneine in an isolated test sample of a subject, and methods for deciding or recommending whether to initiate a therapeutic intervention or for determining the efficacy of a therapeutic intervention. It is also herein provided ergothioneine for use in the treatment and / or prevention of a renal lithiasis or an aminoaciduria, and ergothioneine for use in combination therapy.

The application belongs to the field of biomedical materials, and discloses a gellan gum dressing for treating chronic wounds of diabetes and a preparation method thereof. The dressing takes gellan gum (GG) as a base, loads Zn / Ce-ergothioneine metal organic compound (Zn / Ce-MOC) and cobalt porphyrin casein nanoparticles (CoPP@PLL-Casein), forms CoPP / MOC@GG hydrogel, and is the gellan gum dressing. The dressing is aimed at the complex pathological microenvironment of chronic wounds of diabetes, generates reactive oxygen under light, realizes efficient antibacterial and antibiofilm effects, catalytically decomposes hydrogen peroxide to generate oxygen, and effectively relieves local hypoxia of the wound. Meanwhile, the dressing can eliminate excess free radicals, reduce oxidative stress damage, and has the ability to accelerate healing of wounds of diabetes.

The present application relates to the field of functional fiber and textile finishing technology, and particularly relates to a kind of washable skin-friendly synergistic modified bedding functional fiber and its preparation method.Functional fiber includes base fiber material, biomimetic adhesion dynamic crosslinking ion association modified polymer, organic small molecule functional regulator and polyether modified silicone oil, fatty alcohol polyoxyethylene ether, glyoxal, citric acid and antioxidant 1010.Biomimetic adhesion dynamic crosslinking ion association modified polymer is formed by polydopamine adhesion layer through oxidation self-polymerization of dopamine hydrochloride, and constructs dynamic crosslinking structure with protoacraldehyde and polyethylene imine, while introducing quaternary phosphonium salt modifier to form ion association structure;Organic small molecule functional regulator is ergothioneine.Through synergistic modification, a stable composite modified layer is constructed on the surface of the fiber, and the effective fixation of functional components is realized.The preparation method of the present application is simple and controllable, and the obtained functional fiber has excellent washable performance and skin-friendly performance, and is suitable for bedding field.

This invention discloses an ergothioneine-curcumin synergistic anti-inflammatory and anti-aging composite composition and its preparation method, relating to the fields of biomedicine and functional skincare technology. This invention constructs a ternary nanolipid carrier core-shell hierarchical structure consisting of a hydrophobic core, an interface-enhancing layer, and a hydrophilic shell. The hydrophobic core, formed by hydrogenated lecithin and phytosterols, provides a stable lipid microenvironment for curcumin. The oil-water interface region of the lipid bilayer utilizes its polar gradient to directionally anchor piperine molecules from black pepper extract, allowing piperine to be precisely positioned at the lipid membrane interface. The hydrophilic shell, composed of thiolated hyaluronic acid, covalently connects ergothioneine via thiol-disulfide bonds, stably fixing ergothioneine to the carrier surface. This allows it to exert its antioxidant protective effect upon contact with the skin or mucous membranes, while simultaneously preventing the migration and diffusion of ergothioneine into the inner lipid region. This achieves spatially ordered partitioning and functional synergy of the three active ingredients.

This invention discloses a physiologically acceptable salt of ergothioneine and a method for its preparation. The method provided by this invention can produce ergothioneine salt efficiently and stably; moreover, the ergothioneine salt of this invention solves the problem of ergothioneine having an off-flavor, and therefore has broad application prospects in the fields of dietary supplements, food, or cosmetics.

This invention belongs to the field of animal husbandry technology and relates to a method for improving the quality of frozen semen from Lanzhou fat-tailed sheep based on ergothioneine. This application, through optimized combination of diluent, 8 layers of gauze wrapping for cooling, 4 cm fumigation height, and 8 mM ergothioneine, achieved a sperm motility rate of 45.80±3.62%, an acrosome integrity rate of 52.32±2.28%, and a plasma membrane integrity rate of 55.38±3.55% after thawing. All indicators are significantly better than those of the traditional single-optimization group.

This invention relates to the technical field of detecting active ingredients in Ganoderma lucidum and shiitake mushroom oral liquid, and provides a method for simultaneously detecting ergothioneine and ganoderic acid A in Ganoderma lucidum and shiitake mushroom oral liquid, comprising the following steps: detecting Ganoderma lucidum and shiitake mushroom oral liquid using high performance liquid chromatography (HPLC); the HPLC column used is a JADE-PAK NH2 column, the column temperature is 30-40℃, the detector is an ultraviolet detector, and the detection wavelength is 252-256nm; the mobile phase includes mobile phase A and mobile phase B, mobile phase A is an acetonitrile solution, mobile phase B is a 0.1% (V / V) aqueous solution of acetic acid, the volume ratio of mobile phase A to mobile phase B is (74-76):(24-26), and the flow rate of the mobile phase is 0.9-1.1 mL·min. ‑1 This invention utilizes a JADE-PAK NH2 amino column for the detection of Ganoderma lucidum and shiitake mushroom oral liquid, and through optimization of chromatographic conditions, enables accurate and rapid simultaneous determination of the contents of ganoderic acid A and ergothioneine in Ganoderma lucidum and shiitake mushroom oral liquid.

The present application relates to the field of daily chemical products, and specifically discloses a supermolecule of ergothioneine, which is a co-crystal of ergothioneine and carnosine, wherein the molar ratio of ergothioneine to carnosine is 3:1 to 1:2. The supermolecule of the present application not only solves the problems of instability and easy degradation of ergothioneine, but also improves the skin permeability of ergothioneine, and synergistically enhances the antioxidant effect and has the anti-glycosylation effect. Therefore, it has the effects of anti-glycosylation, skin lightening, whitening, antioxidant, anti-UV, anti-early aging or anti-aging, etc., and can be used for preparing skin external agents, foods, food raw materials, daily chemical raw materials or pharmaceutical raw materials. In addition, the preparation method of the supermolecule of the present application is simple, has the advantages of high yield, low cost and suitability for large-scale production.

The application discloses a composition for inhibiting degradation and odor of ergothioneine, which is compounded by ergothioneine, liquorice extract and cyclodextrin, and the mass ratio of the ergothioneine, the liquorice extract and the cyclodextrin is 1:(0.5-20):(5-200). The composition can improve the stability of the ergothioneine under high-temperature light, light and normal-temperature conditions in a solution and skin care products, inhibit degradation of the ergothioneine, and obviously improve product odor; in addition, the liquorice extract is a plant-derived extract, is widely applied and has good safety, and triterpenoids such as glycyrrhizic acid contained in the liquorice extract can synergistically improve the antioxidant effect of the ergothioneine, so that the composition can be well applied in the field of cosmetics. The application further discloses an application of the composition for inhibiting degradation and odor of ergothioneine.

The application provides a torulopsis pullulans engineering strain and a construction method and application thereof, and belongs to the field of microorganisms and food biotechnology. The application provides a torulopsis pullulans DL-XKX01 engineering strain with high ergothioneine production capacity. The RNA interference technology is used to inhibit the carotenoid synthesis pathway which competes with ergothioneine for substrates, so as to improve the ergothioneine production capacity. The ergothioneine level of the torulopsis pullulans DL-XKX01 provided by the application can reach 116.24+ / -4.33 mg / L in a flask fermentation, and can reach 858.18+ / -5.23 mg / L in a fermenter. The torulopsis pullulans DL-XKX01 can be used to improve the ergothioneine content in fermented food and improve the food quality, and has a good industrial application prospect.

The application relates to the field of pet anti-aging and antioxidant medicines, in particular to a pet anti-aging and antioxidant special nutrient supplement and a preparation method thereof. The pet anti-aging and antioxidant special nutrient supplement comprises the following components in parts by weight: NMN 25-35 parts, manganese ascorbate 3-5 parts, coenzyme Q10 30-40 parts, hydrolyzed tannin 5-8 parts, Omega-3 fatty acid 180-220 parts, ergothioneine 2-3 parts, L-carnitine 20-40 parts, betaine 3-5 parts and vitamin E 5-8 parts. The various substances in the formula realize the pet anti-aging and antioxidant functions through a multi-pathway synergistic mechanism, which respectively are direct free radical scavenging, construction of an antioxidant defense network, repair of mitochondrial function, improvement of cell energy metabolism efficiency, blockage of inflammation and metabolic disorder and cutting off of an aging associated pathway.

The present application relates to a boletus planting culture medium and a preparation method thereof, and belongs to the technical field of biology. The boletus planting culture medium is prepared by using bagasse fermented by Clostridium cellulolyticum, Halanaerobium sp. and Leuconostoc citreum as a culture medium raw material, and combining the bagasse with cottonseed hulls, bran, corn flour, gypsum powder, glucose, potassium dihydrogen phosphate and magnesium sulfate. The boletus cultivated by using the culture medium can effectively improve the mycelium growth of boletus, the time of boletus mushroom growth, the yield, the content of crude protein, polysaccharide and ergothioneine in the fruiting body, and the quality of boletus.