585 results about "Ergothioneine" patented technology

The invention provides recombinant escherichia coli with high yield of ergothioneine and a method for preparing ergothioneine, the recombinant escherichia coli co-expresses ergothioneine anabolic enzymes Egt1 and Egt2, the methyltransferase structural domain of the Egt1 is derived from trichoderma reesei, mycobacterium smegmatis EgtD or rhizopus nigricans, and the methyltransferase structural domain of the Egt2 is derived from the trichoderma reesei, the mycobacterium smegmatis EgtD or the rhizopus nigricans. The histidine sulfoxide synthase structural domain is derived from trichoderma reesei, schizosaccharomyces pombe or rhizopus nigricans, and the Egt2 is derived from claviceps purpurea, neurospora crassa or aspergillus niger. After the recombinant escherichia coli obtained by screening is cultured for 68 hours in a 5L fermentation tank, the yield of ergothioneine can reach 5g / L or above; wherein after recombinant escherichia coli co-expressing Egt1 enzyme (the structural domain of methyltransferase is derived from mycobacterium smegmatis EgtD, and the structural domain of histidine sulfoxide synthase is derived from trichoderma reesei) and Egt2 enzyme (derived from neurospora crassa) are cultured in a 5L fermentation tank for 68 hours, the yield of ergothioneine can reach 8.7 g / L, and the production intensity reaches 127.9 mg / (L.h).

The invention discloses a hydroxytyrosol-ergothioneine eutectic crystal as well as a preparation method and application thereof, and belongs to the technical fields of medicines, foods, daily chemicals and the like. The invention provides a hydroxytyrosol-ergothioneine eutectic crystal. The X-ray powder diffraction pattern of the hydroxytyrosol-ergothioneine eutectic crystal has characteristic diffraction peaks at least corresponding to the positions where the 2 theta value is 4.21 degrees + / -0.2 degrees, 8.36 degrees + / -0.2 degrees, 12.29 degrees + / -0.2 degrees, 15.29 degrees + / -0.2 degrees, 19.40 degrees + / -0.2 degrees, 20.98 degrees + / -0.2 degrees, 23.12 degrees + / -0.2 degrees and 28.73 degrees + / -0.2 degrees. According to the hydroxytyrosol-ergothioneine eutectic, the melting point of hydroxytyrosol is increased, the hygroscopicity of hydroxytyrosol is remarkably reduced, and meanwhile the stability of hydroxytyrosol and ergothioneine is enhanced. In addition, after the ergothioneine hydroxytyrosol forms the eutectic crystal, the ergothioneine hydroxytyrosol can also play better anti-oxidation and anti-aging effects.

The invention belongs to the technical field of health-care food, and provides a composition containing ergothioneine and having sleep-aiding, whitening, anti-aging and anti-inflammatory effects and application thereof. The ergothioneine composition disclosed by the invention is prepared from ergothioneine, collagen peptide, carnosine, nicotinamide, an olive leaf extract and a chamomile extract. The components in the composition are matched for use, the synergistic effect is obvious, the whitening, anti-aging and anti-inflammatory effects are obviously improved, meanwhile, the composition also has a sleep-aiding effect, and the comprehensive performance is excellent.

The invention relates to an anti-oxidation composition based on a reverse micelle synergistic technology and a preparation method and application thereof, the anti-oxidation composition comprises a reverse micelle structure and an aqueous solution containing an anti-oxidation active matter, and the reverse micelle structure wraps the aqueous solution containing the anti-oxidation active matter. The reverse micelle structure is used for wrapping the water-soluble antioxidant active matter, oxidation or degradation discoloration of the water-soluble antioxidant active matter is prevented, after the antioxidant active matter such as hydroxytyrosol, ergothioneine and dipotassium glycyrrhizinate is wrapped by the reverse micelle, the stability and the effect are remarkably improved, and after the reverse micelle wrapping, the effect of the antioxidant active matter is improved by 17% or above compared with an aqueous solution before wrapping.

The invention discloses an ovarian protection composition containing ergothioneine and a preparation method of the ovarian protection composition, and relates to the technical field of ovarian protection compositions. The ovary protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.1 to 0.5 part of the ergothioneine, 0.05 to 1 part of S-equol, 1 to 5 parts of reducing coenzyme Q10, 1 to 3 parts of vitamin E, 10 to 20 parts of chitosan oligosaccharide, 30 to 50 parts of microcrystalline cellulose, 3 to 8 parts of hydroxypropyl methyl cellulose, 0.5 to 2 parts of silicon dioxide and 0.5 to 2 parts of a stabilizer. By adopting the stabilizer, photooxidation and hydrolysis of sensitive components are inhibited through molecular action, and the long-term stability of the composition in a humid and hot environment is remarkably improved in combination with the physical barrier action of silicon dioxide. Through the antioxidant synergistic effect of the ergothioneine and the vitamin E, the hormone regulation effect of the S-equol and the energy metabolism improvement effect of the coenzyme Q10, a multi-layer ovarian function protection system is constructed, and the health state of ovaries is improved. Through a special process, the tablet can be rapidly disintegrated and slowly released in an intestinal targeted manner, burst release of active ingredients is avoided, and the absorption efficiency is improved.

The invention discloses a liposome vesicle for inhibiting ergothioneine degradation and a preparation method thereof, and belongs to the technical field of cosmetics. According to the ergothioneine liposome and the preparation method thereof disclosed by the invention, by constructing an embedding structure, the ergothioneine is embedded in a phospholipid bimolecular layer of the liposome vesicle, and the outer layer of the phospholipid bimolecular layer is also wrapped with a biocompatible high-molecular polymer shell, so that the ergothioneine is doubly wrapped by the phospholipid bimolecular layer and the high-molecular polymer shell of the ergothioneine liposome; and the degradation of ergothioneine is reduced. And the phospholipid bilayer base material for preparing the liposome vesicles can generate ionic interaction with trimethylamine degraded by the ergothioneine, so that the odor problem of the ergothioneine is effectively reduced.

The invention discloses a preparation method of an oral liver protection composition containing ergothioneine, and relates to the technical field of ergothioneine application. The oral liver protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.5 to 5 parts of the ergothioneine, 10 to 30 parts of silibinin, 20 to 50 parts of a phospholipid complex, 5 to 15 parts of a liver targeting carrier, 2 to 8 parts of a disintegrating agent, 1 to 5 parts of an adhesive and 2 to 4 parts of an antioxidant. Free radicals are efficiently captured through a polycyclic conjugated pi electron system of the antioxidant, a hydrogen bond network is formed by a heteroatom bridging effect of the antioxidant, a galactose group of the liver targeting carrier and the disintegrating agent, and the stability of the system is synergistically improved; the phospholipid complex is embedded with a hydrophobic drug and a hydrophilic carrier, and the drug is accurately delivered to the liver by combining the specific recognition capability of the liver targeting carrier; the ergothioneine and the silybin form a bidirectional synergistic liver protection mechanism by removing reactive oxygen free radicals and regulating and controlling cell signal channels respectively.

The invention discloses a fusion protein which comprises a first structural domain with an EgtD catalytic function, a second structural domain with an Egt1 catalytic function and a third structural domain with an EgtE catalytic function, and the amino acid sequence of the first structural domain with the EgtD catalytic function is shown as SEQ ID NO.1; the amino acid sequence of the second structural domain with the Egt1 catalytic function is as shown in SEQ ID NO. 2; and the amino acid sequence of the third structural domain with the EgtE catalytic function is as shown in SEQ ID NO. 3. The fusion protein is subjected to heterologous expression in engineering bacteria, so that the yield of ergothioneine is remarkably increased.

The invention discloses a genetically engineered bacterium and application of the genetically engineered bacterium in preparation of ergothioneine. The genetically engineered bacterium carries genes egtB, egtD and egtE for coding ergothioneine synthetase, and the genes egtB, egtD and egtE are assembled with a protein scaffold assembly SGP; the protein scaffold assembly SGP comprises a protein scaffold GBD, a protein scaffold SH3 and a protein scaffold PDZ. The inventor finds that the ergothioneine synthetases egtB, egtD and egtE are assembled by using the protein scaffold assembly SGP, so that the ergothioneine can be efficiently synthesized, and the protein scaffold assembly has an important application prospect.

The invention discloses a bacteriophage defense system as well as a construction method and application of the bacteriophage defense system in anti-bacteriophage engineering bacteria. The bacteriophage defense system is derived from Escherichia coli Nissle 1917, and comprises proteins as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, and derived proteins of the proteins as shown in SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. According to the invention, a Type II restriction-modification system with strong bacteriophage defense capability is identified from Escherichia coli Nissle 1917, and the Type II restriction-modification system is transplanted into strains such as Escherichia coli W3110, MG1655 (DE3), BL21 (DE3) and the like, so that the strains are endowed with superstrong bacteriophage resistance. The system is successfully applied to industrial engineering bacteria for producing ectoine, fine glufosinate-ammonium, ergothioneine and other products, and the bacteriophage defense capability of the industrial engineering bacteria is effectively enhanced on the basis that the production capacity of the industrial engineering bacteria is not seriously influenced.

The invention discloses a Yarrowia lipolytica strain for synthesizing ergothioneine and application of the Yarrowia lipolytica strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a key enzyme TrEGT1 is subjected to semi-rational transformation, so that the ergothioneine yield of a strain expressing a mutant is increased by 240.9%, precursor metabolic network screening is further modularized, precursor flux is enhanced, a competitive approach of a precursor is knocked out and weakened, and strategies such as fermentation process optimization and the like are further optimized; and when the constructed strain is fermented in a 5L bioreactor, the yield of ergothioneine can reach 10.3 g / L. The highest-level ergothioneine synthesis reported at present is realized, the feasibility of synthesizing the ergothioneine by using the Yarrowia lipolytica as the chassis cell is verified, and a reference value is provided for efficiently synthesizing the ergothioneine by using other chassis cells.

The invention belongs to the technical field of nano-enzymes, and particularly relates to a CuPH (at) KMO composite nano-enzyme material as well as a preparation method and application thereof. Aiming at the problems of poor catalytic activity, complex steps, high raw material cost and low biocompatibility of nano-enzyme prepared by the existing method, the preparation method comprises the following steps: by taking terephthalic acid and histidine as dual-coordination copper-based nano-enzyme as a precursor, introducing manganese oxide in situ under a hydrothermal condition; and the flower-like nano mimic enzyme which is uniform in size and has high laccase-like catalytic characteristics at the same time is synthesized. The composite nano-enzyme provided by the invention can catalytically oxidize phenol amine organic compounds under the condition that the pH value is 3-9, can realize the detection of the content of ergothioneine in cosmetics and nutritional supplement capsules, and can be applied to the field of cosmetic and food safety monitoring.

The invention discloses a method for efficiently producing ergothioneine by metabolic engineering modified escherichia coli and application, and belongs to the technical field of biology. By adopting the recombinant escherichia coli EGT-1-B * provided by the invention, glucose can be converted into ergothioneine; therefore, the raw materials are cheap and easy to obtain, additives and heavy metals which are harmful to human health are avoided, a good foundation is laid for later product purification, and the requirements of commercial application can be met. Furthermore, genes related to metabolism of histidine, methionine, cysteine and S-adenosylmethionine are further overexpressed in the recombinant escherichia coli EGT-1-B *, so that the content of precursor amino acid synthesized by the ergothioneine is increased, and meanwhile, after the gene yqaB is knocked out, the yield of the ergothioneine is further increased; the utilization rate of glucose to ergothioneine can be further improved, and the yield of ergothioneine can reach 140.29 mg / L.

The invention discloses a composition for treating prostatitis and a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 5 to 15 parts of ergothioneine, 10 to 20 parts of cistanche total glycosides, 25 to 45 parts of phytosterol, 450 to 550 parts of pumpkin seed oil, 180 to 250 parts of fructus cannabis oil and 250 to 350 parts of auxiliary materials. Through the synergistic effect of various natural active components, the composition maintains the balance of overall metabolism of a body while reducing inflammation and inhibiting prostate cell proliferation, side effect risks possibly brought by a traditional single-component treatment scheme are avoided, and the composition has higher clinical application potential. The composition disclosed by the invention has a wide market application prospect and popularization value by virtue of good safety and compliance.

The invention belongs to the technical field of medicines, and particularly relates to a multi-effect composition containing ergothioneine, a preparation method of the multi-effect composition and application of the multi-effect composition in eye products. The multi-effect composition is prepared from ergothioneine, tromethamine and sodium hyaluronate according to the mass ratio of (0.5 to 10) to (1 to 5) to (0.5 to 3). According to the multi-effect composition containing the ergothioneine, the tromethamine in the composition can protect the stability of the ergothioneine under the conditions of illumination and high temperature; the ergosulfide can protect the activity of macromolecular sodium hyaluronate. The synergistic compatibility of the ergothioneine, the sodium hyaluronate and the tromethamine is utilized, so that the moisturizing effect and the eye protection effect of the composition can be remarkably improved. The ergothioneine-containing multi-effect composition provided by the invention has the effect of preventing cataract through specific components and proportions, and has the advantages of long-acting eye moistening and good stability.

The invention discloses an oral joint protection composition containing ergothioneine and a preparation method thereof, and relates to the technical field of joint protection compositions. The oral joint protection composition containing the ergothioneine is prepared from the following components in parts by mass: 0.05 to 5 parts of the ergothioneine, 20 to 50 parts of glucosamine salt, 10 to 30 parts of chondroitin sulfate, 2 to 10 parts of curcumin, 30 to 70 parts of a diluent, 1 to 10 parts of an adhesive, 0.5 to 5 parts of a lubricant, 2 to 10 parts of a disintegrating agent and 0.1 to 0.5 part of an antioxidant stabilizer. Through synergistic compatibility of active ingredients such as ergothioneine, curcumin and the like, a multi-dimensional action network of oxidation resistance, inflammation resistance and cartilage metabolism regulation is formed, the inhibition effect on joint inflammation is remarkably improved, and the limitation of a single ingredient is broken through.

The invention discloses eye drops containing L-ergothioneine and a preparation method of the eye drops. The eye drops are prepared from the following components in parts by weight: 0.0015 to 15 parts of L-ergothioneine, 0.001 to 0.01 part of a bacteriostatic agent, 0.05 to 15 parts of a buffering agent and the balance of normal saline, and the pH value of the eye drops is 5.0 to 7.0. Experiments show that L-ergothioneine can prevent and reverse denaturation of crystalline lens protein, the prepared eye drops containing L-ergothioneine can prevent and reduce opacity of crystalline lens protein, and the prevention and treatment effects on cataract are better than those of existing eye drops.

The invention discloses a genetic engineering strain with high yield of ergothioneine, a construction method of the genetic engineering strain and a method for producing ergothioneine, and belongs to the technical field of ergothioneine synthesis. The invention solves the problems of low ergothioneine yield and high production cost in the prior art. According to the genetic engineering strain with high yield of ergothioneine, streptomyces fradiae is used as an original strain, and ergothioneine synthesis genes POegt1 and POegt2 are simultaneously over-expressed in the original strain. The gene engineering strain is cultured to produce high-yield ergothioneine, and the production cost is saved. The genetic engineering strain provided by the invention is suitable for production of ergothioneine.

The invention belongs to the technical field of nutritional compositions and cosmetics, and particularly relates to an anti-aging composition and application and a product thereof. The composition provided by the invention is composed of L-ergothioneine and alpha-ketoglutaric acid, or L-ergothioneine and alpha-ketoglutaric acid salt, or L-ergothioneine and alpha-ketoglutaric acid salt. Through the synergistic effect of the L-ergothioneine and the alpha-ketoglutaric acid or the alpha-ketoglutarate, the contents of Col I and hydroxyproline in skin tissues can be remarkably increased, the expression of TGF-beta1 is up-regulated and converted, the activity of MMP-1 is inhibited, meanwhile, the malondialdehyde level is reduced to enhance the oxidation resistance, the synthesis of hyaluronic acid is promoted, and the water content of the skin is increased. The composition is simple in component and simple and convenient in preparation process, can be prepared into various products such as cosmetics, health care products or medicines, is used for achieving the effects of resisting aging, moisturizing, resisting inflammation or resisting oxidation, particularly has an excellent improvement effect on skin photoaging induced by ultraviolet rays, and is wide in application prospect.

The invention aims to disclose a special dietary preparation based on alpha-ketoglutaric acid as well as a preparation process and application thereof, and relates to the technical field of dietary supplements. Every 800 mg of the dietary preparation comprises 50 mg to 100 mg of alpha-ketoglutaric acid or alpha-ketoglutaric acid salt, 5 mg to 12 mg of N-acetylneuraminic acid or N-acetylneuraminic acid salt, 30 mg to 60 mg of ergothioneine, 100 mg to 150 mg of a desert cistanche deserticola water extract or 50 mg to 80 mg of desert cistanche deserticola total glycosides, and auxiliary materials. The alpha-ketoglutaric acid salt is any one or more of alpha-ketoglutaric acid calcium, alpha-ketoglutaric acid sodium and alpha-ketoglutaric acid magnesium; the N-acetylneuraminic acid salt is any one or more of N-acetylneuraminic acid calcium, N-acetylneuraminic acid sodium and N-acetylneuraminic acid magnesium; the special dietary preparation has the beneficial effects that the special dietary preparation simultaneously contains alpha-ketoglutaric acid or alpha-ketoglutaric acid salt, N-acetylneuraminic acid or N-acetylneuraminic acid salt, ergothioneine and the cistanche deserticola aqueous extract, and has the effects of delaying senescence, resisting fatigue, improving the symptom of kidney-yang deficiency and enhancing immunity for males aged more than 45 years old.

The invention discloses a composition for delaying cell senescence, and the composition for delaying cell senescence comprises fisetin, urolithin A and ergothioneine, or consists of fisetin, urolithin A and ergothioneine. The invention also discloses an application of the composition for delaying cell aging in preparation of a product for delaying skin fibroblast aging, and an anti-aging preparation containing the composition. According to the composition for delaying cell aging, fisetin, urolithin A and ergothioneine contained in the composition can generate a synergistic effect, aging of skin fibroblasts can be remarkably delayed, and healing and repairing of skin are promoted.

The invention provides an anti-aging composition and application thereof, the composition at least contains pyrroloquinoline quinone disodium salt, ergothioneine and 5-aminolevulinic acid or salt thereof in physiologically acceptable media, the composition contains PQQ, ergothioneine and 5-aminolevulinic acid (5-ALA) or salt thereof, the three components are compounded to achieve a synergistic effect, and the anti-aging effect is achieved. The dyeing intensity of beta-galactosidase is reduced, and the anti-aging effect is achieved.

The invention relates to the technical field of skin care products, and discloses an anti-aging and whitening composition and a preparation method thereof.The anti-aging and whitening composition comprises anti-aging and whitening components, whitening synergistic components, moisturizing and repairing components and deionized water, and the anti-aging and whitening components comprise botryine, ergothioneine and Ectoine; wherein the mass ratio of the glassine to the ergothioneine to the ectoine is (1.2 to 2.0) to (0.4 to 0.7) to (1.0 to 1.5); the whitening synergistic component comprises one or more of nicotinamide, glabridin and tranexamic acid; the moisturizing and repairing component is prepared from one or more of sodium hyaluronate, ceramide NP, glycerol and 1, 3-butanediol. Experiments show that the mass ratio of the glassine to the ergothioneine to the ectoine is (1.2-2.0): (0.4-0.7): (1.0-1.5), so that the glassine, the ergothioneine and the ectoine show a remarkable synergistic effect (in some cases, the synergistic index CIlt is 0.85), and the anti-aging and whitening functions of the composition containing the glassine, the ergothioneine and the ectoine are improved.

The invention provides a composition for delaying senescence by activating telomerase activity. The composition is prepared from the following raw materials in parts by weight: 12.5 parts of wheat germ concentrated powder, 0.25 to 5 parts of ergothioneine, 0.25 to 5 parts of pyrroloquinoline quinone disodium salt and 0.25 to 5 parts of alpha-ketoglutaric acid. The invention further provides a preparation method and application of the composition for delaying senescence by activating telomerase activity. Through the synergistic effect of the ergothioneine and the pyrroloquinoline quinone disodium salt, the telomerase activity can be activated, the telomerase expression can be improved, the telomere length can be maintained, and the effect of delaying senescence can be achieved, so that the functional metabolism of a human body can be promoted, and the health state of the body can be improved; through the synergistic effect of the wheat germ concentrated powder and alpha-ketoglutaric acid, cell aging can be delayed; through the multi-target synergistic effect of'telomerase activation + mitochondrial repair + autophagy enhancement ', the anti-aging effect is delayed, the metabolic capability is high, the anti-aging effect is good, and safety and high efficiency are achieved; belongs to the technical field of food and health care product processing.

The invention provides a method for separating and purifying ergothioneine by simulated moving bed chromatography, which comprises the following steps: step 1, carrying out pretreatment, sterilization and ultrafiltration on fermentation liquor to obtain ultrafiltrate; 2, separating and purifying the ultrafiltrate by adopting a simulated moving bed chromatography system, and collecting a target component containing ergothioneine; and step 3, carrying out deep desalination, decoloration, concentration, cooling crystallization, centrifugation and drying on the target component to obtain a pure ergothioneine product. According to the method for separating and purifying the ergothioneine through the simulated moving bed chromatography, in the ergothioneine preparation process, the ergothioneine is purified from fermentation liquor through the simulated moving bed technology, and the method is high in efficiency and capable of achieving automatic and continuous large-scale production.

The invention provides a culture medium containing ergothioneine and application of the culture medium in promoting growth of probiotics, and particularly relates to the technical field of microorganisms. The invention finds that the ergothioneine can promote the growth of the probiotics for the first time, and the verification finds that the ergothioneine has the effects of shortening the growth time, improving the bacterial growth rate, increasing the bacterial quantity and promoting the strain proliferation on various probiotics.

The invention discloses a low-temperature storage and preservation method for litchis. The method comprises the following steps of: removing branches and leaves of fresh litchis which are 80-90% mature, and reserving bare litchis; the fruits pass through a sorting line, and defective fruits are picked out; and soaking the lychee in an ergothioneine and mannitol solution, draining water, packaging the lychee with a moisture-permeable and air-permeable functional fresh-keeping bag, and storing the lychee at the temperature of-1 DEG C to 5 DEG C. According to the fresh-keeping method combining ergothioneine, mannitol and a moisture-permeable and air-permeable functional fresh-keeping bag, the fresh-keeping period of the litchis can be effectively prolonged through natural oxidation resistance and hydration and by matching with humidity adjustment and gas exchange, and the flavor, texture and nutritional ingredients of the litchis are kept. The method not only meets green and environment-friendly requirements, but also can remarkably reduce loss of litchis in transportation and storage, reduce food waste and improve food safety, and is an advanced and efficient fruit fresh-keeping technology.

The invention belongs to the field of agricultural food and biological medicine, discloses a method for producing ergothioneine by fermentation of edible mushroom boletus edulis and application, and comprises a method for producing ergothioneine by liquid fermentation of the edible mushroom boletus edulis and a method for separating and purifying ergothioneine from a fermentation product in a high-yield and high-purity manner. By optimizing the composition of the fermentation culture medium, the supply of precursor substances and the discharge of products are enhanced, and the ergothioneine fermentation yield of the boletus edulis is remarkably increased and is increased by 419.3% compared with that of a control group. According to the invention, a serial adsorption process combining D941 anion exchange resin with 732 cation exchange resin is provided, and a gradient elution process combining ammonia water / ethanol solution is combined, so that pigments and other impurities in the fermentation liquor are effectively removed, the high yield and high purity of the ergothioneine separation and purification process are guaranteed, the purity of a separated sample reaches 96.71%, and the total yield reaches 99.13%. The method has the characteristics of greenness, environmental protection, high safety, simple and convenient process flow and the like, and shows a huge industrial application prospect.

The invention provides a novel crystal form of ergothioneine and a preparation method thereof, and belongs to the technical field of medical crystallization. The method for preparing the new crystal form of ergothioneine provided by the invention is simple to operate, low in energy consumption and high in economical efficiency, the product has good chemical stability, the yield is more than 90%, the purity reaches more than 99.90%, the preparation process is stable, the reproducibility is high, and the method has a good application prospect.

The invention belongs to the technical field of health care products or daily chemical products, and particularly relates to a composition with anti-wrinkle, firming, whitening and brightening effects and application. The composition is prepared from the following components in parts by weight: 72 to 180 parts of fisetin, 120 to 180 parts of urolithin A and 5 to 15 parts of ergothioneine. Through precise component compounding, the synergistic interaction among fisetin, urolithin A and ergothioneine is brought into full play, the skin care composition integrating anti-wrinkle, firming, whitening and brightening functions is successfully developed, and the natural components of the skin care composition ensure the use safety of the product, so that the skin care composition has a good application prospect. The requirements of consumers on efficient skin care are effectively met.