205 results about "Ergothioneine" patented technology

The invention relates to a strain Pleurotus ostreatus TIB.BPE.10010 with the accession number being CGMCC No.6232. The invention also relates to a method of preparing L-erythrothioneine. The method comprises steps of culturing and fermenting the Pleurotus ostreatus CGMCC No.6232 to perform biosynthesis to form the L-erythrothioneine and extracting the L-erythrothioneine from mycelium cells.

The invention provides a method for preparing ergothioneine. The method takes mushrooms or leftovers as raw materials and comprises the following steps: extracting the raw materials in water or an ethanol water solution with the volume percent concentration of 5 percent to 30 percent at the temperature of 70 DEG C to 95 DEG C; sequentially separating and purifying an extracted product through macroporous resin and polyamide resin; collecting an eluting solution and concentrating to obtain ergothioneine extract. Compared with the prior art, the extract with the ergothioneine content of 20 percent to 98 percent can be obtained and the content is much higher than 1 percent to 5 percent in the prior art. The ergothioneine prepared by the invention can reach requirements of industrialized production and lays a foundation for further development and utilization of the ergothioneine extract.

A composition and methods of use are disclosed for prevention and control of inflammation and oxidative stress and various associated medical conditions, including PTSD, chronic depression and traumatic brain injury. A composition providing a phytonutrient and enriched mushrooms having enhanced Vitamin D and ergothioneine are disclosed. The combined use of an enriched mushroom and phytonutrient provide a synergistic effect on cellular longevity and/or cellular rejuvenation of subjects with both a normal and nutritionally deficient diets, improved tolerance to oxidative and/or inflammatory stress, and increased cellular longevity and/or cellular rejuvenation as a result of the neutralization of free radicals and prevention of chronic inflammation.

The invention relates to a biosynthesis preparation method for ergothioneine, and a fermentation culture medium. The method comprises the following steps of S1: inoculating Hericium erinaceus myceliumslant cultures to a liquid seed culture medium to be cultured to obtain seed liquid; S2: inoculating the obtained seed liquid to the fermentation culture medium to be fermented, adding precusor substances for culturing, and fermenting to a fermentation destination through pH (Potential of Hydrogen) judging a fermentation liquor; S3: after fermentation finishes, adding enzyme, carrying out enzymolysis to the destination, raising the temperature to kill enzyme, and lixiviating the ergothioneine in the cell of the mycelium to the fermentation liquor out of the cell. By use of the method disclosed by the invention, the yield of the ergothioneine can be improved, the fermentation destination can be quickly and simply judged, fermentation byproducts are reduced, the enzyme is used for breakingmycelium cells, and ergothioneine is safe to be lixiviated.

A method for extracting and purifying L-ergothioneine comprises the following steps: (1) soaking and extracting a mushroom mycelium fermentation liquid by using hot water, so as to obtain a mushroom and L-ergothioneine water solution; (2) performing ultrafiltration of the mushroom and L-ergothioneine water solution by using an ultrafiltration membrane with a molecular weight cut-off of 1kDa to 30kDa, and collecting an ultrafiltration liquid; and (3) performing chromatographic purification of the ultrafiltration permeation liquid, the chromatographic purification conditions being: a chromatographic filler being an HILIC filler, a mobile phase forming acetonitrile-water with a volume ratio of 80: 20 to 88: 12, and a quantity of feed of L-ergothioneine being 0.01g to 0.323 per 100g.

The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) An enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5'-phosphate as a coenzyme.

The invention discloses a composition containing L-Ergothioneine extract, brown rice fermentation filtrate and acetylchitosamine and application of the composition. The composition includes 0.1-2.0 parts of L-Ergothione extract, 0.1-4.0 parts of brown rice fermentation filtrate and 0.1-2.0 parts of acetylchitosamine. Since the three natural fermentation source components which include the L-Ergothioneine extract, brown rice fermentation filtrate and acetylchitosamine are selected, the composition has the advantages of naturalness, high activity, easy absorption and high safety, an obvious synergistic effect is achieved through sieving of the dosage of the components, meanwhile, the effects of regulating the biological rhythm of skin, delaying skin aging and improving pigmentation are achieved, and damage of blue light to the skin is prevented from the three aspects, so that comprehensiveness is achieved, and the blue light resistant effect is achieved.

Personal care composition including a first skin and/or hair care active cetyl pyridinium chloride; and at least one additional skin and/or hair care active selected from the group consisting of tetrahydrocurcumin, sugar amine, vitamin B₃, retinoids, hydroquinone, peptides, phytosterol, dialkanoyl hydroxyproline, hexamidine, salicylic acid, n-acyl amino acid compounds, sunscreen actives, water soluble vitamins, oil soluble vitamins, hesperedin, mustard seed extract, glycyrrhizic acid, glycyrrhetinic acid, carnosine, Butylated Hydroxytoluene (BHT) and Butylated Hydroxyanisole (BHA), ergothioneine, vanillin or its derivatives, diethylhexyl syrinylidene malonate, melanostatine, sterol esters, idebenone, dehydroacetic acid, Licohalcone A, creatine, creatinine, feverfew extract, yeast extract, beta glucans, alpha glucans, their salts, their derivatives, their precursors, and/or combinations thereof; and a dermatologically acceptable carrier. The invention further relates to methods for regulating the condition of mammalian keratinous tissue wherein the methods each comprise the step of topically applying to the keratinous tissue of a mammal needing such treatment, a safe and effective amount of the personal care composition of the invention.

The present invention relates in general to methods of protecting a mammalian central nervous system cell from damage, and to methods of treating or ameliorating neurodegenerative diseases. The invention further relates to screening for neuro-protective agents that may alone, or in combination with other neuroprotective agents, aid in protecting cells of the central nervous system from damage attributed to neurotoxic compounds, free radicals, or neurodegenerative diseases. The invention further relates to pharmaceutical compositions comprising L-ergothioneine or other newly identified compounds and pharmaceutically acceptable carriers for administration to a mammal in need of neuroprotection.

Personal care compositions containing ergothioneine are provided. Methods for regulating the condition of mammalian keratinous tissue by topically applying the personal care compositions are also provided.

The invention provides a method for inhibiting photodegradation of ergothioneine and application of the method. The method comprises the following steps: adding hyaluronate into a solution containingergothioneine; the content of the ergothioneine is 0.0005-0.01% by weight; and the content of the hyaluronate is more than 0.1% by weight. According to the present invention, the combination of hyaluronate and ergothioneine can effectively inhibit the photodegradation of ergothioneine, and the composition can be use in food health products or cosmetics, with good moisturizing effect and stable efficacy.

The invention relates to a composition for repairing skin barriers and improving skin resistance and a preparation method. The composition comprises the following raw material components: 2-3 parts ofa paeoniae radix extract, 1-2 parts of a leontopodium alpinum extract, 3-5 parts of a bifida ferment product extract, 5.5-9.6 parts of biomimetic polypeptide, 12-16 parts of a solvent, 3-4 parts of beta-glucan, 1-2 parts of ergothioneine, 0.1-0.5 part of hyaluronic acid, 0.15-0.6 part of hydrolyzed sodium hyaluronate and 0.3-0.4 part of a preservative. The composition for repairing the skin barriers and improving the skin resistance has the beneficial effects that the paeoniae radix extract, the leontopodium alpinum extract and the bifida ferment product extract are compounded with bionic polypeptide, so that the composition has the effects of enhancing the self-repairing capability of the skin and improving the skin immunity; By adding bionic polypeptide, skin receptor signals can be increased in a multi-channel manner to improve aged skin.

The invention discloses an application of needle mushroom genes Fvegt1, Fvegt2 and Fvegt3 to synthesis of ergothioneine, and belongs to the field of biosynthesis. By a homologous clone method and a gene synthesis method, 3 genes in an ergothioneine synthesis way are cloned from a needle mushroom mycelium, expression in vitro is performed, and in vitro biosynthesis of the needle mushroom ergothioneine is realized. The FvEgt1 can catalyze substrates of His, Cys, SAM, FeSO4 and the like to form AdoHcy and HerSul; FvEgt2 can be combined with the Cys, so that SH of cysteine is activated to form sulfur anions; the FvEgt3 can catalyze PLP and HerSul to form a ketimine compound; and the sulfur anions activated by the SH of the cysteine perform nucleophillic attack on the ketimine compound to obtain the ergothioneine. The invention firstly clarifies a biosynthesis way of the ergothioneine in basidiomycetes needle mushrooms except bacteria, ascomycetes and the like.

The invention provides an efficacy promoting composition, which is characterized by comprising polymer hyaluronic acid or salt thereof, hydrolyzed hyaluronic acid or salt thereof, acetylated hyaluronic acid or salt thereof, tetrahydropyrimidine or derivative thereof, ergothioneine, yeast/rice fermentation product filtrate and aminobutyric acid. When the efficacy promoting composition provided by the invention is used for moisturizing and repairing the skin, on one hand, the stability of macromolecular functional components can be improved, and the contact time with the skin is prolonged, so that the utilization rate of the macromolecular functional components is increased, the dosage of the functional components is reduced, the cost is saved, and skin irritation caused by excessive dosageof some functional components is reduced; on the other hand, the transdermal absorption effect of the micromolecular functional components can be improved, and the utilization rate of the micromolecular functional components is increased.

The invention belongs to the technical field of skin care products, and particularly relates to a moisturizing, repairing, anti-aging and anti-saccharification composition and application thereof. The moisturizing, repairing, anti-aging and anti-saccharification composition is characterized by being prepared from the following raw materials in parts by weight: 0.1-8 parts of vitamin palmitates, 1-6 parts of vitamine, 1-20 parts of a plant extract, 0.1-4 parts of ergothioneine, 0.01-0.5 part of astaxanthin, 1-13 parts of argireline, 0.5-6 parts of fullerene, 0.1-6 parts of a peptide compound, 0.1-7 parts of hydrolyzed collagen, 0.01-0.2 part of an antioxidant active enzyme and 0.1-4 parts of a coenzyme. The composition in the technical scheme is applied to moisturizers, emulsions, creams, essence stock solutions and masks to keep the moisture and elasticity of cells, promote regeneration of damaged tissues, improve the repairing capacity of the skin, delay aging of the cells and improve the anti-aging capacity and anti-saccharification capacity of the skin.

The invention relates to a deep moisturizing compound and a preparation method thereof. The deep moisturizing compound consists of the following components in parts by weight: 0.05-1 part of hyaluronic acid, 0.04-2 parts of transdermal fibronectin, 0.06-4 parts of ergothioneine, 4-40 parts of a dispersing agent and 5-60 parts of a solvent. The preparation method of the deep moisturizing compound comprises the following steps: dispersing the sodium hyaluronate and the ergothioneine by using the dispersing agent according to the ratio, then adding the material into the solvent, stirring the materials until the sodium hyaluronate and the ergothioneine are completely dissolved, finally adding the transdermal fibronectin, and completely stirring the material to obtain the deep moisturizing compound. According to the deep moisturizing compound provided by the invention, hyaluronic acid is used as a main functional substance for skin moisturizing, meanwhile, transdermal fibronectin is added to promote permeation and realize deep moisturizing, and finally ergothioneine is used as an auxiliary material to improve and repair a skin barrier and lock moisture in skin, so that comfortable skinfeeling can be provided, and skin health is facilitated.

The invention discloses an engineering strain for fermented synthesis of ergothioneine and a construction method thereof, and belongs to the technical field of bioengineering. With escherichia coli and bacillus subtilis as the host and three shuttle plasmids pEBS of bacillus subtilis, escherichia coli and saccharomyces cerevisiae as the expression vector, egtABCDE gene clusters from different sources are introduced into the escherichia coli and the bacillus subtilis for heterologous expression; biosynthesis of the ergothioneine is realized; then, the egtABCDE gene clusters are subjected to optimized expression, including codon optimization, promoter optimization and expression of different combinations, and meanwhile a culture medium and added methionine, hercynine, histidine and cysteineare optimized. Through the optimized expression and optimized culture conditions, efficient biosynthesis of the ergothione is realized. The engineering strain lays a foundation for efficient fermentedpreparation of ergothione by a microbial system and is suitable for industrial production and application.

The invention relates to the field of protection of injuries from environmental stress factors, in particular to an efficient moisturizing and allergy-relieving repairing composition and application thereof. The high-efficiency moisturizing and allergy-relieving repair composition comprises the following components in percentage by mass: a) ectoin; b) ergothioneine or a pharmaceutically acceptablesalt or ester thereof; and c) cannabinoid as an active ingredient, wherein the content of the active ingredient in the composition is 0.5-5%. The composition has the effects of relieving sensitivity,repairing skin micro-damage and repairing after sun exposure, and can also be used for repairing a skin barrier after household acid brushing; by matching with 4D hyaluronic acid, the skin can be moistened immediately, an invisible protection film is established, external stimulation is resisted, a skin protective layer is built, the active ingredient is locked, and the purposes of efficiently moisturizing, relieving allergy and repairing are achieved.