The invention discloses a formula having anti-fatigue, anti-premature decrepitude and anti-coagulation effects and a preparation method thereof. The components of the formula include sea perch fish sperms, urechis unicinctus, shelled oysters, antler blood, chaga mushroom, radix ginseng, solomonseal rhizome, cassia bark, lepidium meyenii and Chinese chive seeds. The formula is rich in protein, vitamin A, B vitamins, calcium, magnesium, zinc, selenium, phospholipids, minerals, collagen, proteoglycan, hyaluronic acid, nucleotide, ganglioside, growth hormone and various amino acids required by human bodies, therefore, blood circulation can be promoted, intravascular waste can be timely removed, anti-fatigue, anti-premature decrepitude, anti-coagulant and anti-thrombotic effects can be achieved, unblocked microcirculation can be ensured, and premature ageing can be effectively prevented.