34 results about "Hypotaurine" patented technology

Provided is a method for using low molecular weight compounds found in the body as a biomarker for diagnosing depression. Specifically, more than one compound selected from a group comprising the following are used: ADP-ribose, ATP, ADP, AMP, serotonin, tryptophan, kynurenine, SDMA (symmetrical dimethylarginine), threonine, glyceric acid, serine, N-acetylaspartic acid, glutamic acid, trigonelline, creatine, 2-methylserine, sphingosine, homovanillic acid, piperidine, sulfoxidized methionine, pipecolic acid, sphinganine, gamma-butyrobetaine, guanidinoacetic acid, isobutyric acid, creatinine, sarcosine, 3-methylbutyric acid, nicotinamide, betaine, ornithine, carnitine, ethanolamine, phosphoethanolamine, taurine, hypotaurine, aspartic acid, methionine, and tyrosine.

The present invention discloses a breast cancer diagnostic marker combination and application and a determination method thereof, and particularly relates to 13 difference metabolites (Aspartate, Glutamate, 5-Oxoproline, Cystine, Glutamine, Cysteine, Hypotaurine, Asparagine, Glycerolphosphate, Glycerophosphocholine, Arachidonate, Glyoxylate and pentosidine) and a combination thereof as a breast cancer early detection and diagnosis marker and application thereof and a determination method of the diagnosis marker, and the determination method is a metabonomic analysis method based on liquid / gas chromatography-mass spectrometry of plasma / serum of a patient with breast cancer. The diagnostic marker combination has the characteristics of high sensitivity and high specificity, has high sensitivity and specificity for early diagnosis of breast cancer, can be used for early detection of breast cancer, can gain time for patients to begin treatment as soon as possible, and improves clinical therapeutic effects.

The present invention discloses a cloned embryo treatment liquid, which comprises BIX-01294 and a PZM culture liquid, wherein the BIX-01294 concentration in the PZM culture liquid is 5-500 nM, and 500 mL of the PZM culture liquid comprise 3.156 of NaCl, 1.053 g of NaHCO3, 0.373 g of KCl, 0.024 g of KH2SO4, 0.049 g of MgSO4, 0.308 g of calcium lactate, 0.011 g of pyruvic acid, 0.073 g of L-glutamic acid, 0.273 g of hypotaurine, 10 mL of BME, 5 mL of MEM, 0.033 g of penicillin, 0.025 g of streptomycin, and the balance of Sigma water. According to the present invention, with the application of the cloned embryo treatment liquid using the technical scheme to perform the culture treatment on the cloned embryo, the gene expression of SUV39H1, SETdb1, DNMT1 or DNMT3a in the cell can be significantly reduced, and the H3K9me2 levels of 2cell and 4cell at the early embryo development stage can be significantly down-regulated.

A method of inhibiting enzymatic browning in food involves the contacting of hypotaurine, a salt of hypotaurine or an equivalent of hypotaurine with the food. The hypotaurine can be provided from solution, an extract, or in solid form to a food that can loose its appeal to a consumer due to browning, such as cut fruits and vegetables, seafood, or a beverage. The antibrowning agent can be applied to the food by spraying, dusting, dipping, or dissolving, depending upon the form of the food to which the agent is added.

The invention disclosesculture liquid for improving the development quality of a bovine fertilized embryoin vitro. The culture liquid comprises the following additives of an insulin-transferrin-selenium additive, beta-mercaptoethanol, hypotaurine, 1-oleoyl-sn-glycerol-3-sodium phosphate and salidroside. A culture method for improving the development quality of the bovine fertilized embryoin vitroby using the culture liquid is disclosed. The culture liquid and the culture methodcan effectively improve the development rate and development quality of the bovine fertilized embryoin vitro.

The invention discloses novel markers for predicting the efficacy of pemetrexed-platinum combined treatment on non-small cell lung cancer and its application. Specifically, the invention provides a set of the markers for predicting the efficacy of pemetrexed-platinum combined treatment on non-small cell lung cancer and its use in a product for predicting the efficacy of pemetrexed-platinum combined treatment on non-small cell lung cancer. The markers comprise hypotaurine, uridine, dodecanoyl carnitine, choline, dimethyl glycine, nicotinamide and hexadecanoyl carnitine. The markers and a modelwith the markers can predict the efficacy of the chemotherapy scheme before the patient undergoes chemotherapy and has sensitivity of 90.8% and specificity of 79.5%.

The invention relates to a method for preventing and treating brown rot of peaches. The method comprises the following steps: soaking peaches in hypotaurine solution at the concentration of 5-20 ppm for 0.5-2 h, processing and storing. The rot rate and the plaque diameter of peaches which have been soaked in the hypotaurine solution are obviously reduced, the activities of relative protective enzymes (phenylalanine ammonia-lyase and chitinase) are obviously increased, and brown rot of peaches is obviously suppressed. The method is safe and efficient, the process is simple, the method can be widely applied in storage of harvested peaches, and thus the method has broad market prospect.

The invention discloses a fertilization solution for in-vitro fertilization of cattle and a use method thereof. The fertilization solution includes a SOF basal medium without inositol, and also includes the following additives: L-arginine, L-aspartic acid, L-carnitine, adrenaline, penicillamine, hypotaurine, and heparin. The fertilization solution of the invention can effectively ensure the in-vitro fertilization rate of the bovine embryo and the development quality of the fertilized embryo.

An evaluating method includes an evaluating step of evaluating a state of lung cancer for a subject to be evaluated using a concentration value of at least one of Homoarginine, GABA, 3-Me-His, ADMA, Spermine, Spermidine, Cystathionine, Sarcosine, aAiBA, bAiBA, Putrescine, N-Acetyl-L-lys, Hypotaurine, bABA, and Ethylglycine in blood of the subject.

The invention belongs to the technical field of high-throughput sequencing data analysis, and particularly relates to a method for screening transcription factors related to postharvest softening of grape fruits. According to the invention, hypotaurine (HT) and hydrogen peroxide (H2O2) treatment is carried out on harvested 'Kyoho' grapes, through transcriptome sequencing and weighted gene co-expression network analysis (WGCNA), correlation analysis is carried out on differential expression genes obtained through transcriptome analysis and pectin content change, a differential expression gene module closely related to the pectin content change is screened out, and the specific value of the differential expression genes and the specific value of the pectin content change are obtained. And excavating differential expression transcription factors in the softening process of the picked grape fruits. According to the method, the softening character related transcription factors can be effectively screened, and a theoretical reference is provided for researching the softening occurrence mechanism of the picked grape fruits.

Provided is a method for using low molecular weight compounds found in the body as a biomarker for diagnosing depression. Specifically, more than one compound selected from a group comprising the following are used: ADP-ribose, ATP, ADP, AMP, serotonin, tryptophan, kynurenine, SDMA (symmetrical dimethylarginine), threonine, glyceric acid, serine, N-acetylaspartic acid, glutamic acid, trigonelline, creatine, 2-methylserine, sphingosine, homovanillic acid, piperidine, sulfoxidized methionine, pipecolic acid, sphinganine, gamma-butyrobetaine, guanidinoacetic acid, isobutyric acid, creatinine, sarcosine, 3-methylbutyric acid, nicotinamide, betaine, ornithine, carnitine, ethanolamine, phosphoethanolamine, taurine, hypotaurine, aspartic acid, methionine, and tyrosine.

The invention relates to a novel application of small molecule metabolites ethanolamine, fumaric acid, hypotaurine and inositol in a serum sample as a combined marker in preparation of a kit for diagnosing whether a diabetic has retinopathy or not. The invention also relates to a kit for detecting retinopathy in diabetic patients, which is used for calculating the variable Prob value of the combined marker and judging the cut-off value (cut-off value) on the basis of a binary logistic regression equation by detecting the respective relative concentrations of the combined marker in the serum of the diabetic patients, and judging whether the diabetic suffers from retinal complications or not. The kit can realize high-sensitivity and high-efficiency detection of several small molecule metabolites involved in the invention, and has the characteristics of low detection cost and good repeatability. The method can be applied to auxiliary diagnosis of retinal complications in diabetic people, has a good effect in early diagnosis of diabetic retinopathy, and has a good application prospect.

The invention provides a composition comprising N-acetyl-D-tryptophan and hypotaurine and a kit for evaluating oocyte quality. The kit is used as a standard in an oocyte quality evaluating system, andthe quality of oocyte is evaluated by comparing the contents of the N-acetyl-D-tryptophan and the hypotaurineand in follicular fluid and corresponding components in the composition. The composition can be used as a basis for selecting high-quality oocyte for an IVF-ET technology, embryos with higher quality are obtained for transplantation, thereby increasing the pregnancy rate and reducing abortion, and the composition is of great significance for the birth of healthy offspring. Compared with existing morphological evaluation methods, the composition and the kit can be used for achieving non-invasive, objective and convenient oocyte quality evaluation.

An evaluating method includes an evaluating step of evaluating a state of colorectal cancer for a subject to be evaluated using (i) a concentration value of at least one metabolite of bABA, N-Me-bABA, 1-Me-His, aABA, Aminoadipic acid, bAiBA, Cadaverine, Ethylglycine, GABA, Homoarginine, Hypotaurine, Kinurenine, N6-Acetyl-L-Lys, Putrescine, Serotonin, Spermidine, Spermine, ADMA, Homocitrulline, 3-Me-His, Hydroxyproline, Phosphoethanolamine, SDMA, Acylcarnitine (13:1), and EPA in blood of the subject or (ii) a value of a formula calculated using the concentration value of the metabolite and the formula including an explanatory variable to be substituted with the concentration value of the metabolite.

The present invention describes an approach to produce taurine or increase hypotaurine or taurine production in prokaryotes or eukaryotes. More particularly, the invention relates to genetic transformation of organisms with algal, microalgal or fungal genes that encode proteins that pool catalyze the conversion of sulfur-containing compounds such as sulfate or cysteine to taurine. The invention describes methods for the use of polynucleotides for cysteine dioxygenase-like (CDOL), sulfmoalanine decarboxylase-like (SADL), cysteine sulfate / decarboxylase or a portion of the cysteine synthetase / PLP decarboxylase (partCS / PLP-DC) polypeptide in bacteria, alga, yeast, or plants to produce taurine or increase hypotaurine or taurine. The preferred embodiment of the invention is in plants but other organisms may be used. Taurine production or increased levels of hypoataurine or taurine in plants could be used as nutraceutical, pharmaceutical, or therapeutic compounds or as a supplement in animal feed or for animal feed or as an enhancer for plant growth or yield

An evaluating method includes an evaluating step of evaluating future risk of developing Alzheimer's disease for a subject to be evaluated having mild cognitive impairment using a concentration value of at least one of α-ABA, Ala, Arg, Asn, Cit, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Orn, Phe, Pro, Ser, Thr, Trp, Tyr, Val, Cysteine, Taurine, bABA, Ethylglycine, Hypotaurine, 3-Me-His, 5-HydroxyTrp, aAiBA, and N8-Acetylspermidine in blood of the subject.

The invention relates to the application of a reagent for inhibiting the biosynthesis of endogenous metabolite-hypotaurine in the preparation of drugs for inhibiting tumor cell proliferation. Wherein said agents that inhibit the biosynthesis of endogenous metabolite hypotaurine include, but are not limited to, homocysteine ​​and taurine. The present invention also relates to interfering with the biosynthesis of hypotaurine, an endogenous metabolite of α-ketoglutarate-dependent dioxygenases (α-Ketoglutarate-dependent dioxygenases), to inhibit the growth of tumor cells Methods.

The invention discloses a bacteriostatic uterine horn insemination diluent suitable for donkey semen, and also discloses a preparation method of the antibacterial uterine horn insemination diluent suitable for donkey semen. The advantages are: by adding an appropriate amount of taurine, hypotaurine, bovine serum albumin, heparin sodium and progesterone, it can promote sperm capacitation in vitro and maintain sperm motility. It can greatly improve the utilization rate of male donkey semen, and further increase the number of fertilized female donkeys, so that more female donkeys can be conceived and promote donkey breeding. The development and expansion of the enterprise's breeding scale will improve the economic benefits of the enterprise; by adding an appropriate amount of cefoxitin sodium, amphotericin and polymyxin, it can effectively reduce the probability of metritis in female donkeys, and ensure a healthy uterine environment for female donkeys. Promoting the application and promotion of deep angle insemination can provide a strong guarantee for improving the conception rate.

The invention relates to a method for preventing and treating peach fruit brown rot. The steps of the method are as follows: the peach fruit is soaked in a hypotaurine solution with a concentration of 5-20 ppm for 0.5-2 h, treated, and then stored. The decay rate and plaque diameter of peach fruits treated with hypotaurine acid were significantly reduced, and the activities of defense-related enzymes (phenylalanine ammonia lyase and chitinase) were significantly increased, which significantly inhibited the peach fruit browning. rot. The method is safe, efficient and simple in process, can be widely applied to the storage of post-harvest peach fruits, and has broad market application prospects.

The invention relates to a method of treating and preventing ageing of the pilosebaceous unit and / or alopecia in human through the administration of taurine and / or hypotaurine and / or the acceptable salts thereof in an oral composition. Specifically, the invention relates to a method of treating and preventing miniaturization of the hair follicle. The invention also relates to the use of polyphenols to achieve these same ends. Finally, the invention relates to oral compositions that include polyphenols or taurine and / or hypotaurine and / or the acceptable salts thereof as active ingredients.

The present invention pertains to a cell culture medium comprising hypotaurine, GABA, and / or beta-alanine or the combination of choline and hypotaurine, GABA, and / or beta-alanine as media supplements which is shown to control viability, growth, and waste byproduct accumulation. The present invention further pertains to a method of producing a polypeptide of interest in a large scale cell culture containing hypotaurine, GABA, and / or beta-alanine or the combination of choline and hypotaurine, GABA, and / or beta-alanine.

The present invention describes an approach to produce taurine or increase hypotaurine or taurine production in prokaryotes or eukaryotes. More particularly, the invention relates to genetic transformation of organisms with algal, microalgal or fungal genes that encode proteins that pool catalyze the conversion of sulfur-containing compounds such as sulfate or cysteine to taurine. The invention describes methods for the use of polynucleotides for cysteine dioxygenase-like (CDOL), sulfinoalanine decarboxylase-like (SADL), cysteine sulfate / decarboxylase or a portion of the cysteine synthetase / PLP decarboxylase (partCS / PLP-DC) polypeptide in bacteria, alga, yeast, or plants to produce taurine or increase hypotaurine or taurine. The preferred embodiment of the invention is in plants but other organisms may be used. Taurine production or increased levels of hypoataurine or taurine in plants could be used as nutraceutical, pharmaceutical, or therapeutic compounds or as a supplement in animal feed or for animal feed or as an enhancer for plant growth or yield.

An evaluating method includes an evaluating step of evaluating a state of mild cognitive impairment for a subject to be evaluated, using (i) at least one value of concentration values of Put, α-ABA, Ala, Arg, Asn, Cit, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Orn, Phe, Pro, Ser, Thr, Trp, Tyr, Val, Taurine, 1-MeHis, 3-MeHis, aAAA, aAiBA, ADMA, Allyl Cys, bABA, bAiBA, Cystathionine, Cysteic acid, EtGly, GABA, hArg, Hypotaurine, Hypro, Kyn, Pyrazole-1-Ala, MCSO, MeCys, N6-AcLys, N8-AcSpd, Opthalmic acid, PEA, Pipecolic acid, Sar, SDMA, Spd, Spm, and Thioproline and test values of Alb, Folate, WBC, RBC, Hb, Ht, and PLT in blood of the subject, or (ii) a value of a formula including an explanatory variable to be substituted with the at least one value, calculated using the at least one value and the formula.

The invention discloses a cloned embryo treatment solution, which is composed of BIX-01294 and PZM culture solution, the concentration of BIX-01294 in the PZM culture solution is 5nM-500nM; wherein, the PZM culture solution is composed of the following components: NaCl 3.156g; NaHCO 3 1.053g; KCl 0.373g; KH 2 SO 4 0.024g; MgSO 4 0.049g; calcium lactate 0.308g; pyruvate 0.011g; L-glutamic acid 0.073g; hypotaurine 0.273g; BME 10mL; MEM 5mL; penicillin 0.033g; 500mL. Using the cloned embryo treatment solution of the above technical scheme to culture and treat cloned embryos can achieve the following beneficial effects: (1) can significantly reduce the gene expression of cells SUV39H1, SETdb1, DNMT1 or DNMT3a; (2) can significantly down-regulate the H3K9me2 levels in 2cell and 4cell.

The invention discloses new application of mogroside V and a biomarkers of the mogroside V acting on lung inflammation. The first application: the mogroside V is used for preparing medicine for treating the lung inflammation. The second application: the mogroside V serves as an inhibitor and is used for improving and reducing the increase of the content of TNF-alpha, IL-4 and IgE inflammatory factors and the reduction of the content of IFN-gamma caused by OVA stimulation, and inflammatory infiltration in lung tissue is relieved. The third application: the mogroside V is used for preparing the medicine for relieving cough or relieving cough and moistening lung. The invention also researches a biomarker of mogroside V acting on lung inflammation, wherein the biomarker is taurine, hypotaurine, pyridoxine, 5-phosphopyridoxal, histidine and aspartic acid. The invention provides new application of the mogroside V. The mogroside V has the effects of eliminating lung inflammation and moistening the lung to arrest cough, is low in toxic and side effects, can be used for development of clinical cough and lung moistening medicines, and is wide in market prospect.

An evaluating method includes an evaluating step of evaluating a state of pancreatic cancer for a subject to be evaluated using (i) a concentration value of at least one metabolite of N-Me-bABA, bABA, GABA, N6-Acetyl-L-Lys, 1-Me-His, aABA, Aminoadipic acid, bAiBA, Cadaverine, Ethylglycine, Homoarginine, Hypotaurine, Kinurenine, Putrescine, Serotonin, Spermidine, Spermine, ADMA, Homocitrulline, 3-Me-His, Hydroxyproline, Phosphoethanolamine, Acylcarnitine (13:1), and EPA in blood of the subject or (ii) a value of a formula calculated using the concentration value of the metabolite and the formula including an explanatory variable to be substituted with the concentration value of the metabolite.

A method of inhibiting enzymatic browning in food involves the contacting of hypotaurine, a salt of hypotaurine or an equivalent of hypotaurine with the food. The hypotaurine can be provided from solution, an extract, or in solid form to a food that can loose its appeal to a consumer due to browning, such as cut fruits and vegetables, seafood, or a beverage. The antibrowning agent can be applied to the food by spraying, dusting, dipping, or dissolving, depending upon the form of the food to which the agent is added.