1646 results about "Rational design" patented technology

Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed.

The present invention overcomes the inadequacies inherent in the known methods for generating libraries of antibody-encoding polynucleotides by specifically designing the libraries with directed sequence and length diversity. The libraries are designed to reflect the preimmune repertoire naturally created by the human immune system and are based on rational design informed by examination of publicly available databases of human antibody sequences.

The present invention overcomes the inadequacies inherent in the known methods for generating libraries of antibody-encoding polynucleotides by specifically designing the libraries with directed sequence and length diversity. The libraries are designed to reflect the preimmune repertoire naturally created by the human immune system and are based on rational design informed by examination of publicly available databases of human antibody sequences.

Gene expression profiling reveals four distinct subgroups of multiple myeloma that have significant correlation with various clinical characteristics. Diagnosis for multiple myeloma (and possibly monoclonal gammopathy of undetermined significance) based on differential expression of 14 genes, as well as prognosis for the four subgroups of multiple myeloma based on the expression of 24 genes are established. A 15-gene model that classifies myeloma into 7 groups is also reported. Gene expression profiling also allows placing multiple myeloma into a developmental schema parallel to that of normal plasma cell differentiation. Development of a gene expression- or developmental stage-based classification system for multiple myeloma would lead to rational design of more accurate and sensitive diagnostics, prognostics and tumor-specific therapies for multiple myeloma.

Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for INCENP.

Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for CDKN1B.

Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for Nup62.

Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to COPB2.

Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to PKN-3.

A method for the rational design and preparation of vaccines based on HIV envelope polypeptides is described. In one embodiment, the method for making an HIV gp120 subunit vaccine for a geographic region comprises determining neutralizing epitopes in the V2 and / or C4 domains of gp120 of HIV isolates from the geographic region and selecting an HIV strain having gp120 a neutralizing epitope in the V2 or C4-domain which is common among isolates in the geographic region. In a preferred embodiment of the method, neutralizing epitopes for the V2, V3, and C4 domains of gp120 are determined. At least two HIV isolates having different neutralizing epitopes in the V2, V3, or C4 domain are selected and used to make the vaccine. The invention also provides a multivalent HIV gp120 subunit vaccine. A DNA sequence encoding gp120 from preferred vaccine strains of HIV, GNE8 and GNE16, expression constructs comprising the GNE8-gp120 and GNE16-gp120 encoding DNA under the transcriptional and translational control of a heterologous promoter, and isolated GNE8-gp120 and GNE16-gp120 are also described.

Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed.

The present invention features inter alia altered binding polypeptides having engineered cysteine residues or analogs thereof at a predetermined site within, for example, a constant region domain or a portion thereof. The engineered cysteine residues or analogs thereof provide sites for conjugating effector moieties (e.g. diagnostic or therapeutic agents) that impart novel functionality to the binding polypeptide, preferably without interfering with a desirable property (e.g. an Fc-mediated effector function). The invention includes methods for the rational design of such altered polypeptides, as well as methods for modifying (ie. conjugating) the altered polypeptides with desirable effector moieties. Particular modified binding polypeptides (ie. immunoconjugates) of altered binding polypeptides and methods for utilizing such modified binding polypeptides as protein-based therapeutics are also provided.

The invention discloses an experimental device and an experimental method for hydrothermal catalytic pyrolysis in a heavy oil layer by means of ultrasonic wave, wherein the experimental device comprises a core holder, a ring pressure supply device, a water storage tank, an oil storage tank, an ultrasonic transducer, an ultrasonic generator, a liquid container, a catalyst, a reaction assistant storage device and a constant temperature box; a steam generator is arranged on a water pipe between the water storage tank and the core holder; the experimental method comprises the following steps: 1, pre-treating tested core; 2, performing water-flooding experiment and measuring original water-flooding permeability of the tested core; 3, performing oil-flooding experiment and measuring original oil saturation degree of the tested core; 4, performing hydrothermal pyrolysis simulation experiment; and 5, preparing data. The experimental device and the experimental method have rational design. The device is convenient to install and distribute, has complete function, is convenient to use and operate and has good usage effect. The problems that the prior normal steam thermal driving heavy oil has easily-rebounding viscosity and short measure validity period, and the normal hydrothermal catalytic pyrolysis technology has limited effect during the heavy oil recovery process can be solved.

The invention discloses a full-automatic down feather filling machine, which consists of a fan, a material loading mechanism, a down feather storage mechanism, a metering mechanism, a down feather filling mechanism and a material cleaning mechanism. The air inlet of the fan is connected with the material loading mechanism, the material loading mechanism is connected with the down feather storage mechanism, the down feather storage mechanism is connected with the metering mechanism, the metering mechanism is connected with the down feather filling mechanism, the down feather filling mechanism is connected with the material cleaning mechanism, and the material cleaning mechanism is connected with the air outlet of the fan. The working mode of manually filling down feather is replaced by the full-automatic down feather filling machine with the structure, and the down feather flows in a full-enclosed environment in the overall process, so that the working environment of staff is improved, labor intensity is lowered, the waste of the down feather is reduced, and working efficiency is improved. In addition, the full-automatic down feather filling machine has the advantages that: the machine has a simple structure and a rational design and is convenient to use.

A connecting mechanism of vibration table three-directional shocking platform comprises force a passing mechanism which enable the platform for placing sample connect the vibration table at lateral side adjacent to the platform and bottom side of the platform respectively. Said force passing mechanism comprises a sliding block and a horizontal sliding track and a vertical sliding track which are respectively connected to the upper and lower end surfaces of the sliding block. Said connecting mechanism of vibration table three-directional shocking platform has rational design, low making and maintaining cost, and can satisfy the experimental request for achieving simultaneous vibration three-axially by using the three-directional shocking platform. And the connecting mechanism is able to achieving restoration of actual vibration environment furthest, which has light weight, small volume and be easy to assemble and maintain and manage. The mechanism is able to simplify the device of the entire simultaneous shocking apparatus three-axially, and the mechanism does not generate producing source such as public hazard noise and so on.

The invention relates to a high-strength wear-resistant steel plate and a preparation method thereof. The steel comprises the following chemical components in percentage by mass: 0.12 to 0.22 percent of C, 0.25 to 0.50 percent of Si, 1.10 to 1.80 percent of Mn, 0.025 to 0.055 percent of Als, 0.30 to 1.00 percent of Cr, 0.20 to 0.60 percent of Ni, 0.10 to 0.50 percent of Mo, 0.010 to 0.050 percent of Ti, 0.0010 to 0.0050 percent of B, less than or equal to 0.020 percent of P, less than or equal to 0.015 percent of S and the balance of Fe and inevitable impurities. The invention also provides the preparation method of the steel grade. The wear-resistant steel of the invention has the advantages of rational design of the chemical components, no Nb and V component systems, simple rolling process, on-line quenching process, quick production rhythm and stable product performance and can be widely applied to mechanical equipment under various extremely severe working conditions.

The invention is directed to a contact lens design and methods of manufacturing, fitting and using such lenses. The contact lens may be designed for use in a corneal refractive therapy (CRT) program. The lens provides a design which allows proper fitting of a patient, whether for corrective contact lenses or for a CRT program. Due to the rational design of the lenses according to the present invention, a minimal number of lens parameter increments can be identified to cover the range of common corneas. It is therefore possible to provide pre-formed lens buttons or blanks which are easily formed into a final design, thereby simplifying and speeding up the treatment process. Further, any adjustment of the lens design which may be required based upon trial fitting or the like, is easily envisioned and implemented by the fitter.

The invention discloses an ore coarse crushing device for mineral processing, which comprises a bottom plate, a left vertical plate and a right vertical plate are vertically and fixedly installed on the bottom plate, a limit slideway is vertically arranged on the top plate, and the vertical limit slideway is vertically The sliding type is equipped with a sliding rod, and a transmission rod is connected between the sliding rod and the runner. The piston head is connected with a piston rod extending to the left to the outside of the water-drawing sleeve. The left end of the piston rod is fixed with a push plate. There is a nozzle connected to the water-drawing sleeve through the liquid outlet pipe; the structure of the invention is ingenious and the design is reasonable, and the ore in the crushing tank is broken through the up and down reciprocating motion of the impact block, and the left and right reciprocating motion of the crushing tank makes the ore evenly spread on the ground. The bottom of the crushing tank makes the ore get a comprehensive impact crushing effect and improves the crushing efficiency of the ore; the nozzle sprays water intermittently into the crushing tank to reduce the dust generated during the crushing process of the ore, avoiding the spread of dust, and more Environmental friendly.

The invention discloses a construction method of a rigid primary support of mould-building concrete of a plateau permafrost tunnel. The method comprises the following steps: 1. measuring and paying off; 2. tunnel excavation and construction: excavating by utilizing a full face excavation method; 3. construction of the rigid primary support of the mould-building concrete: erecting a grille steel frame, applying an anchor rod, hanging a reinforced mesh and pouring concrete with an initial lining trolley, namely constructing the primary support of the tunnel by utilizing a bolt-mesh-shotcreting-grouting combined support method; 4. laying of a waterproof and thermal insulation layer: laying the waterproof and thermal insulation layer on the completely constructed rigid primary support of the mould-building concrete; and 5. secondary lining construction: carrying out secondary lining construction on the laid waterproof and thermal insulation layer. The construction method has the advantages of rational design, low cost, high engineering quality and fast construction speed, is convenient in construction, effectively ensures the construction safety and quality, and simultaneously ensures that the completely constructed plateau permafrost tunnel has excellent water resistance and drainage functions, excellent thermal insulation function, long service life, low maintenance cost and low later-stage rework cost.

The invention provides a method for remedying soil polluted with cadmium by using interplanting. In the soil polluted with cadmium, cadmium high-accumulation plants and cadmium low-accumulation plants are planted simultaneously in an interplanting manner and planted alternatively at the number ratio of 1 to 1; four plants around each plant are all different from the plant in variety, the plant spacing is 10 to 30 cm and the line spacing is 10 to 30 cm; the plants are watered by using a water source without being polluted by heavy metal and the field moisture capacity of the soil is kept to be 80%; compound fertilizers and carbamide are applied, depending on the soil property and the crop variety; edible parts of the interplanted plants are harvested at the harvest time and the cadmium content in the plant variety with relative low cadmium accumulation is decreased to the specified safe standards for consumption in China, so that the purpose of yielding safe edible products from the soil polluted with cadmium is achieved. The method for remedying soil polluted with cadmium by using interplanting, provided by the invention, has the advantages of rational design, low expense and strong operability and can be applied to the purpose of yielding products at the same time of remedying the soil polluted cadmium.

The invention relates to an intelligent valve positioner. A copper waterproof connector and a silencer are arranged outside a box body, while a transducing plate, a connecting plate, a cartridge valve and an insert block are arranged in the box body; a plug block is arranged below the connecting plate, and a main board is arranged above the connecting plate; a protection cover is arranged above the main board; a valve plate is arranged on one side of the cartridge valve and has a valve port; and a linear displacement sensor is arranged on a pneumatic actuating mechanism out of the box body and connected with the copper waterproof connector by a lead. The intelligent valve positioner has a simple structure, rational design, low energy consumption and high control precision, can conduct functions of valve position alarm, fault alarm and self-diagnosis, can be switched manually and automatically, has 'gas, power supply and signal interruption' protection functions, works stably in severe spot environments and has a wide application range.

The invention discloses a method for detecting a bridge impact coefficient based on a dynamic load test. The method comprises the steps of: (1) detecting and recording deflection data of a bridge; (2) drawing a deflection schedule curve of the bridge; (3) obtaining a bridge impact coefficient muij corresponding to each pair of adjacent wave peak value Xij and wave trough value Yij on the i-th deflection schedule curve of the bridge; (4) obtaining a bridge impact coefficient mui corresponding to a vehicle speed vi; (5) repeating the step (3) and the step (4) until n bridge impact coefficients mu1, mu2, ...mun corresponding to n different vehicle speeds v1, v2, ...vn are obtained; and (6) obtaining a bridge impact coefficient mu. The method for detecting the bridge impact coefficient based on the dynamic load test, disclosed by the invention, has the advantages of rational design and convenience in realization; the bridge impact coefficient obtained by detection is safer, so that dynamic effect of the bridge structure can be reflected more exactly and more rationally under the function of the load of movable vehicles; the practical problem that the conventional impact coefficient cannot reflect exactly that the movable load acts on the bridge structure in form of multiple load points is solved efficiently; and the method for detecting the bridge impact coefficient based on the dynamic load test, has strong practicability and high popularization and application values.

The invention relates to a low voltage ride through control method for a three-phase network connection photovoltaic inverter. The method is characterized in that the output voltage of the inverter is controlled by a network voltage direct feed-forward control policy, whether a network voltage dip fault happens or not is judged by detecting an instantaneous value of the network voltage, a mean network voltage value is directly fed forward when the network voltage is in the range of normal values, and the network voltage direct feed-forward control policy is adopted when the network voltage dip is detected. The low voltage ride through control method for the three-phase network connection photovoltaic inverter has a rational design, the problem that a network connection photovoltaic inverter outputs overcurrent at the beginning of the network voltage dip is solved, and the safe low voltage ride through of the network connection photovoltaic inverter can be realized.

An electric engine starter in which downsizing and weight saving are improved to an extreme based on the rational design and by eliminating an unnecessary unit, wherein a battery is arranged outside the starter, a power accumulating unit has a rotation supporting unit for supporting one end of a spring, a first gear is formed on the rotation supporting unit, a second gear is fixed to an output shaft of a high-speed reduction gear mechanism, the first and the second gears are engaged with each other, the power accumulating unit and the power transmission unit are arranged on the same first axis line, the compact electric motor and the high-speed reduction gear mechanism are arranged on the same second axis line parallel to the first axis line, and the first axis line and the second axis line are arranged on the same plane surface.

Disclosed herein are novel antimicrobial peptides with useful, improved, or superior properties such as antimicrobial activity, desirable levels of hemolytic activity, and therapeutic index against a broad range of microorganisms including gram-negative and gram-positive bacteria and other organisms having a cellular or structural component of a lipid bilayer membrane. Also provided are methods of making and using such peptides to control microbial growth and in pharmaceutical compositions for treatment or prevention of infections caused by such microorganisms. Certain peptides are disclosed utilizing a structure-based rational design relating to an antimicrobial peptide, V681, with single D- / L-amino acid substitutions or charged residue substitutions in or near the center of the peptide on the nonpolar or polar face. Also disclosed are peptides with one or more amino acids in the D configuration, including peptides with all amino acids in the D configuration. Modified peptide analogs herein can demonstrate one or more properties such as improved antimicrobial activity, specificity, and resistance to degradation. Compositions disclosed herein have useful clinical potential as antibiotics including broad spectrum antibiotics.

Gene expression profiling reveals four distinct subgroups of multiple myeloma that have significant correlation with various clinical characteristics. Diagnosis for multiple myeloma (and possibly monoclonal gammopathy of undetermined significance) based on differential expression of 14 genes, as well as prognosis for the four subgroups of multiple myeloma based on the expression of 24 genes are established. A 15-gene model that classifies myeloma into 7 groups is also reported. Gene expression profiling also allows placing multiple myeloma into a developmental schema parallel to that of normal plasma cell differentiation. Development of a gene expression- or developmental stage-based classification system for multiple myeloma would lead to rational design of more accurate and sensitive diagnostics, prognostics and tumor-specific therapies for multiple myeloma.

The present invention involves the structure modification of dynamic power assembly suspending device used for vehicle damping and noise reducing. It includes inner pipes and outer pipes installed out inner pipes, it is characterized by: a central pipe is installed between said pipe and outer pipe, the first elastic body is installed between the inner pipe and central pipe and the second elastic body is installed between the central pipe and outer pipe. Thereby, the advantages of the invention as follows: 1. rational design, simple structure, it can effectively reduce vibration and noise in various conditions; especially the damping and noise reducing effect is good under the idle condition, general acceleration condition and condition; 2, good work stability, reasonable forcing situation, it greatly reduces the stress concentration phenomenon significantly, and effectively extends the use life; 3. convenient assembly, low production cost and easy use .

The invention discloses a triaxial vibration-decreasing / vibration-isolating platform of a hammer crusher, wherein, the whole platform structure belongs to the space redundancy 3-DOF translational mechanism, converting the spatial vibration caused by all excitation forces during the working process of the hammer crusher to triaxial wire vibration by the constraint of the space redundancy 3-DOF translational mechanism, then converting the triaxial wire vibration of a moving platform to uniaxial wire vibration of a mechanical active pair through mutual transitive relations between the moving platform of space redundancy 3-DOF translational mechanism and kinematics and mechanics of the mechanical active pair; buffering and vibration isolation are realized by elastic cells of the active pair and decreasing vibration can be realized through consuming vibrational energy of the system by damping elements of the active pairs, frictional damping of all kinematic pairs, or damping elements connected directly with the moving platform, thus reducing the spatial vibration of the hammer crusher and harm to ambient environment effectively, economically and reliably. The invention has the advantages of rational design, light weight, low cost, small volume and easy assembly and maintenance management, etc.