The invention provides a bispecific
chimeric antigen receptor targeting an HIV-1
virus envelope
protein. The
chimeric antigen receptor comprises (1) a recognition unit targeting an HIV-1 gp120
protein co-
receptor binding site; (2) a recognition unit targeting other binding sites of HIV-1 gp120 (including but not limited to a CD4
binding site, a V1V2
glycan region, a V3
glycan region, a gp120-gp41 interface or a proximal membrane end external region on gp41); (3) a hinge and
transmembrane region; and (4) an
intracellular signal transduction domain; optionally, the
chimeric antigen receptor further comprises (5) one or more co-stimulatory
signal domains; preferably, according to the series connection sequence of the chimeric
antigen receptor extracellular recognition domain, the far membrane end is a recognition unit of a targeted HIV-1 gp120
protein co-
receptor binding site, and the near membrane end is a recognition unit of targeted HIV gp120 other binding sites. The invention provides a brand-new construction method of the HIV-1
virus envelope protein bispecific chimeric
antigen receptor, immune cells modified by the HIV-1
virus envelope protein bispecific chimeric
antigen receptor have stronger activating and killing capabilities, HIV-1 infected cells can be specifically recognized and killed, and the HIV-1 virus envelope protein bispecific chimeric
antigen receptor has a good application prospect.