The invention discloses a multi-technology integrated rapid
ginseng breeding method and application, and the method comprises the following steps: S1, explant screening and gradient sterilization: selecting
rhizome buds of three-year-old healthy
ginseng as explants, firstly soaking the explants in 75%
ethanol for 30 seconds, then disinfecting the explants with a 0.1%
mercury bichloride solution for 8-10 minutes, then washing the explants with
sterile water for 5-6 times, and carrying out gradient sterilization on the explants; after
moisture is absorbed by sterile
filter paper, inoculating to a
callus induction culture medium, and culturing for 25-30 days at the temperature of 22 + / -1 DEG C under the dark condition, so as to obtain healthy
ginseng calluses; the
callus obtained in the step 1 is subjected to
ray irradiation mutagenesis, the
irradiation dose is controlled to be 20-30 Gy, and the
irradiation rate is 1 Gy / min. According to the method, the defect that a traditional ginseng breeding period is too long can be overcome, the breeding period is shortened, the
callus induction rate, the cluster
bud differentiation rate and the
acclimatization and
transplantation survival rate are greatly improved, precise screening of ginseng strains is achieved, and the industrial application cost is greatly reduced.