414 results about "Grain weight" patented technology

The invention discloses a KASP molecular marker for identifying soybean hundred-grain weight and application thereof, and relates to the technical field of biology and application of an SNP locus, the SNP locus takes a soybean Wm82. A2. V1 genome sequence as a reference genome, the SNP is the 16321243th SNP on a soybean 11 # chromosome, and the SNP is the 16321243th SNP on the soybean 11 # chromosome. Corresponding to the 20th basic group from the 5'terminal of the sequence shown in SEQ ID NO: 1, when the locus is CC homozygosis, the corresponding genotype is A; when the locus is TT homozygous, the corresponding genotype is B; according to the application, soybeans with different hundred-grain weights are screened or screened in an auxiliary mode, and the different hundred-grain weights of the soybeans are as follows: the homozygous soybeans with the genotype A are higher than or candidate to be higher than the homozygous soybeans with the genotype B. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of soybean germplasm or breeding progeny materials with high hundred-grain weight.

The invention discloses application of TaMYB-7B and a coding gene thereof in regulation and control of wheat tiller number, and belongs to the technical field of gene engineering. The invention finds for the first time that the TaMYB-7B gene is a novel gene capable of simultaneously regulating and controlling the tillering number and grain traits of wheat. The TaMYB-7B gene is interfered, knocked out, silenced or mutated, so that the tiller number of a plant can be increased, the grain length is increased, the hundred-grain weight is increased, finally, the yield of a single wheat plant can be increased, and a foundation is laid for wheat yield improvement and character improvement.

Provided is the use of a protein derived from wheat and a biomaterial related thereto in increasing the yield per plant and grain protein content of a crop. The protein is designated as SWEET11, and may be a protein having an amino acid sequence as shown in sequence 9, sequence 6 or sequence 3 in the sequence listing. The wheat SWEET11 protein (TaSWEET11) is over-expressed in the Zhengmai 7698 wheat variety (nationally approved), and it is found that the over-expression of TaSWEET11 can significantly improve the thousand-grain weight of wheat grains and increase the yield. In addition, the over-expression of TaSWEET11 also increases the protein content in the wheat grain, significantly improving the grain quality while increasing the yield. The TaSWEET11 protein can be used for breeding and quality improvement of crops.

The invention discloses a corn kernel size regulation gene X1 as well as an SNP (Single Nucleotide Polymorphism) marker and application thereof. The invention discovers that the X1 gene has the function of regulating and controlling the grain shape and the grain weight for the first time. The invention provides a novel SNP (Single Nucleotide Polymorphism) marker related to the corn grain size, the SNP marker has two alleles AA and GG, and the allele AA is clear to be regarded as an excellent / synergistic allele. The X1 gene provided by the invention can lead to increase of grain types such as the length of a corn grain of a mutant strain compared with that of a control grain; the length and other grain types of the X1 gene overexpression strain are reduced compared with those of the control grain. Precious gene resources are provided for corn breeding, and the application prospect is wide.

The invention belongs to the field of gene engineering, and relates to a rice BRW1 gene and application thereof. The cDNA (complementary deoxyribonucleic acid) sequence of the rice BRW1 gene is shown as SEQ ID NO.1, and the amino acid sequence of the RNA binding protein coded by the rice BRW1 gene is shown as SEQ ID NO.2. The invention discloses the rice RNA binding protein gene BRW1 and the protein coded by the rice RNA binding protein gene BRW1. The rice BRW1 gene controls the grain weight of grains, the thousand grain weight of a knockout mutant of the gene is remarkably increased, and meanwhile the grain length and the grain width are increased. By mutating the BRW1 gene in the invention, the grain weight of rice grains can be increased, which indicates that the genetic improvement of the grain weight and grain shape of rice is facilitated by using the gene.

The invention discloses a wheat thousand seed weight identification method based on an SNP molecular marker and application, and relates to the technical field of genetic engineering, and the wheat thousand seed weight identification method comprises the following steps: A1, taking DNA of a wheat sample to be detected as a template, and carrying out PCR amplification by adopting a KASP primer combination according to any one of claims 2-3 or a kit according to claim 4; a2, judging the thousand seed weight of the wheat sample to be detected according to an amplification result. An SNP site related to the thousand seed weight of a plant is obtained through phenotypic difference analysis and exon capture analysis of the thousand seed weight, a corresponding KASP primer combination is designed for the SNP site, and the thousand seed weight of wheat can be rapidly identified through PCR amplification and genotype analysis: the thousand seed weight of AA genotype wheat is significantly higher than that of GG genotype wheat; the method provides an accurate molecular marking tool for high-yield breeding of wheat, has the advantages of high efficiency, accuracy and suitability for high-throughput detection, and has important significance for increasing the yield of wheat.

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention discloses a molecular marker related to thousand seed weight of wheat, a detection primer and application of the molecular marker and the detection primer, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker TaDTX50-STS is as shown in SEQ ID NO. 1; basic groups from the 502 site to the 661 site of the sequence as shown in SEQ ID NO. 1 have insertion / deletion mutation. The InDel molecular marker related to the thousand seed weight character in a wheat genome is identified and named as TaDTX50-STS, and by detecting the polymorphism or genotype of the InDel molecular marker, the InDel molecular marker can be used for identifying or assisting in identifying the thousand seed weight character of wheat, assisting in breeding dominant varieties with high thousand seed weight of wheat and improving the wheat breeding efficiency.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

The invention discloses application of an OsCPN10a gene in regulation and control of vigor, grain shape and drought tolerance of rice seeds, and belongs to the technical field of rice gene engineering. The invention particularly relates to the research of the OsCPN10a gene in the aspects of regulating and controlling the germination rate, the germination potential, the germination index, the grain length, the grain width, the thousand grain weight, the drought tolerance of the rice seeds and the like. Experimental results show that the grain shape of the OsCPN10a knockout mutant seed is obviously lengthened and widened, and the grain shape of the OsCPN10a overexpression seed is opposite; the yield of the Ocpn10a mutant is higher than that of a TNG67 plant; overexpression of the OsCPN10a has the potential of improving the drought resistance of the rice. The invention reveals that OsCPN10a affects genetic mechanisms such as rice seed vigor, grain length, grain width and storability, provides important gene resources and theoretical basis for genetic improvement of rice, and is beneficial to cultivation of a new rice variety with better grain shape and higher yield.

The invention relates to the field of rice gene engineering, and discloses an application of a GSE3.1 protein and a coding gene thereof in regulating and controlling the grain length, the grain width and the grain weight of rice seeds. According to the invention, a GSE3.1 gene is edited based on a CRISPR / Cas9 technology, a japonica rice variety Zhonghua 11 (ZH11) is introduced by using an agrobacterium-mediated method, and a knockout mutant is obtained through screening. The homozygous knockout mutation of the GSE3.1 gene in the invention leads to reduction of the grain width and grain length of rice seeds and reduction of thousand grain weight. According to the invention, the constructed plant overexpression vector ProActin: GSE3.1 is expressed in wild type ZH11, so that compared with the wild type plant, the seed grain length and grain width of the transgenic plant are obviously increased, and the thousand grain weight is obviously increased. Therefore, the GSE3.1 gene and the encoding protein thereof can regulate and control the size and the weight of the rice seeds, and are of great significance to cultivation of high-yield rice varieties.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker closely linked with a sweet corn kernel weight major QTL (Quantitative Trait Loci) and application of the SNP molecular marker, and belongs to the technical field of SNP molecular markers. The nucleotide sequence of the SNP molecular marker is shown as SEQ ID NO.1, the mutation site is the base of the 242797157th site on the first chromosome of a B73 reference genome (RefGenv4), the base of the site is T, and the sweet corn with the genotype of TT shows the high grain weight character; the basic group of the site is C, and the sweet corn with the genotype of CC shows the character of low grain weight. Based on the SNP molecular marker, a method for identifying the sweet corn grain weight character and a method for screening the sweet corn with the high grain weight character are provided, and the methods are high in accuracy, high in detection speed, easy in result interpretation and low in cost and have important significance in improvement of corn breeding efficiency and large-scale high-yield planting of corn.

The invention discloses application of TaNOP2 protein and a coding gene thereof in wheat breeding. The invention also protects the application of the TaNOP2 protein or the TaNOP2 gene as an inhibition target in plant breeding. The target of the plant breeding is to cultivate plants with increased plant height and / or yield and / or tillering number and / or spike length and / or spike weight and / or grain number per spike and / or thousand grain weight and / or photosynthetic rate and / or grain length and / or grain width and / or grain perimeter and / or grain surface area. The invention provides a new thought and a potential target for wheat molecular breeding, can be used for cultivating high-yield new wheat germplasm, and has industrial popularization value.

The invention discloses a corn ZmMYBR37 gene and an application of the corn ZmMYBR37 gene in regulating and controlling the size of corn kernels. The nucleotide sequence of the ZmMYBR37 gene is as shown in SEQ ID No: 1 in a sequence table. A mutant material with small grains and reduced hundred-grain weight is separated from a corn EMS mutant library, and a mutation site occurs on a function unknown gene ZmMYBR37, so that protein translation is terminated in advance. The information analysis finds that the gene coding protein has a conservative MYB structural domain, and the gene coding protein is speculated to belong to an MYB transcription factor family. Gene expression analysis finds that the gene is high in constitutive expression in corn and high in expression level in leaves and embryos. The corn material overexpressed with the ZmMYBR37 has the advantages that the grain size is increased, and the hundred-grain weight is obviously increased, so that the ZmMYBR37 has the potential of increasing the corn yield.

The invention provides application of an OsRTD1 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsRTD1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the OsRTD1 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of the OsRTD1 gene mutant plant osrtd1 under the drought stress of PEG induction and soil dehydration is remarkably improved, the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced, the relative water loss rate of dehydrated leaves is higher, and the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced. The total grain number, the solid grain number, the total grain weight and the like of the OsRTD1 gene mutant plant osrtd1 under the stress of field drought are obviously higher than those of a wild plant. The OsRTD1 gene is a potential candidate new target for regulating and controlling a rice drought resistance mechanism.

The invention relates to the field of plant genetic engineering, in particular to a rice OsDNG1 protein, a coding gene and application of the rice OsDNG1 protein to regulation and control of grain shape and grain weight. According to the invention, the OsDNG1 gene is knocked out through a CRISPR / Cas9 gene editing technology, so that the length of rice grains can be increased by 4.10%-4.62%, and the thousand grain weight can be increased by 10.86%-11.49%. And if the gene is over-expressed, the grain length is reduced by 1.14%-1.32%, and the thousand grain weight is reduced by 8.24%-8.56%. In the re-sequenced indica rice variety, the indica rice variety without the OsDNG1 gene type is longer in grain length and larger in length-width ratio compared with the variety with the gene type. The invention not only provides a key target for analyzing the molecular mechanism of rice grain morphogenesis, but also provides important theoretical basis and technical support for molecular design breeding of rice grain shape and grain weight, and has remarkable agricultural application value.

The invention belongs to the technical field of plant molecular biology, and discloses an application of a soybean GmSW10 gene in reducing soybean plant height, grain weight and grease content and / or increasing protein content. The CDS sequence of the soybean GmSW10 gene is as shown in SEQ ID NO: 1. Knockout of the soybean GmSW10 gene can directly influence the plant height, the grain weight, the oil content and the protein content of soybean seeds, after the soybean GmSW10 gene is knocked out, the weight of soybean seeds and hundred grains is remarkably reduced, the plant height is reduced, the protein content is increased, and the oil content is reduced, which indicates that the GmSW10 gene is a key gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean, and the GmSW10 gene can be used for preparing the soybean GmSW10 gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean. And a new genetic resource is provided for genetic improvement of soybeans.

The invention discloses application of a wheat nitrogen response gene TaNIA1 in cultivation of high-yield wheat. The invention belongs to the technical field of biology, and particularly relates to application of a wheat nitrogen response gene TaNIA1 in cultivation of high-yield wheat. The protein TaNIA1 disclosed by the invention can be applied to the following aspects: 1) application to regulation and control of plant yield; 2) application in preparation of products for regulating and controlling plant yield; the invention relates to application of TaNIA1 in cultivation of plants with changed yield, application of TaNIA1 in preparation of products for cultivation of plants with changed yield, and application of TaNIA1 in plant breeding. TaNIA1 influences nitrogen absorption and utilization of wheat, so that agronomic traits such as wheat tiller number, grain number per ear, ear length, hundred-grain weight and the like are remarkably increased, and the yield of wheat is finally influenced.

The invention discloses application of beta-glucosidase BnaBG27 in regulation and control of thousand seed weight of rape, and belongs to the technical field of gene engineering. According to the invention, the BGLU27 gene is separated and obtained from brassica napus Westar, and the gene is found to be highly expressed in rape seeds. Experiments find for the first time that the BGLU27 gene positively regulates the thousand seed weight of the rape, and the thousand seed weight of the rape can be increased by utilizing the gene, so that high-yield rape germplasm can be obtained. The method has a very important application prospect in breeding and improvement of high-yield rape varieties.

The invention discloses a rape grain weight regulation gene NGAL3 based on whole genome screening and a breeding application thereof, based on the seed size and grain weight phenotypic value of a cabbage type rape germplasm resource, a candidate gene interval is positioned by using GWAS; the method comprises the following steps: selecting large-grain-weight and small-grain-weight extreme phenotypic materials, performing transcriptome sequencing in a critical period of seed development, and screening differential expression genes; and performing cross comparison on the GWAS candidate gene and the differential expression gene to obtain a key gene for jointly regulating and controlling the size and the grain weight of the seed, and performing functional verification on the key gene. The thousand seed weights of the created homozygous three mutant strains L1-sg1-1-4-5 and L2-sg1-4-8-16 are obviously increased by 46% and 29% compared with those of the wild type strains. The invention provides a new target for high-yield rape breeding, and non-transgenic high-grain-weight germplasm can be created through gene editing or beneficial allelic variation of NGAL3 is selected and enriched under the assistance of molecular markers.

The invention discloses application of a KASP molecular marker in identification or auxiliary identification of soybean hundred-grain weight character, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker auxiliary breeding. According to the application of the KASP molecular marker in identification or auxiliary identification of the soybean hundred-grain weight character, the genotype of the KASP molecular marker is G or A, and the KASP molecular marker is located at the 7703700bp position of the No.6 chromosome of a soybean genome Wm82. A2. V1. The obtained KASP marker for regulating and controlling the hundred-grain weight character gene of the soybean is suitable for rapid screening and molecular marker-assisted breeding of the relative height of the hundred-grain weight of the soybean. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The invention relates to the field of plant growth regulators, in particular to a conditioner group for improving the grain quality and the rice quality of direct seeding rice and a preparation and use method thereof.The preparation method of a conditioner in the initial heading stage comprises the following steps that 1, 6-benayl aminopurine is dissolved in a diluted hydrochloric acid solution in a assisted mode, and a 6-benayl aminopurine solution is obtained; step 2, adding choline chloride into the solution obtained in the step 1, stirring to obtain a mixed solution, transferring the mixed solution into a volumetric flask, and adjusting the pH value; 3, abscisic acid powder is dissolved with absolute ethyl alcohol, volume metering is carried out after dissolution to form a stock solution, and then PH adjustment is carried out. Two conditioners are included and sprayed in the initial heading stage and the full heading stage respectively, glumous flower degeneration can be reduced, the photosynthetic production capacity of the upper three leaves after flowering can be improved, assimilation substances are promoted to be transferred to grains, especially the filling state of vulnerable grains is improved, and the yield of the seedlings is increased. Finally, the grain number, the maturing rate, the grain weight and the yield are improved, meanwhile, the head rice rate of rice can be improved, and the protein content is reduced, so that the palatability of rice is improved.

The invention discloses an SV molecular marker remarkably related to drought tolerance and yield of corn and application of the SV molecular marker, and belongs to the field of molecular marker-assisted breeding. According to the invention, the SV variation of the ZmMETTL gene related to the drought tolerance of the corn is found, and the SV variation is a segment of 822bp deletion variation located at the 174690388-174691209 site of the No.3 chromosome of the B73AGPv5 version of the corn. The SV variation is obviously related to the plant height, the single ear weight and the single ear grain weight of a corn material under normal watering and drought conditions, and can be used for screening materials of which the plant height and the yield are obviously different under the normal watering and drought conditions. The invention provides a new direction for identifying corn drought-resistant germplasm resources and assisting corn drought-resistant molecular breeding.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

Higher LOG L1 transcript abundance correlates with lower grain weight under HNT stress. This is supported by higher grain weight of log l1 mutants relative to wild type plants under HNT stress. This finding provides a genetic resource to increase rice adaptation to warming nights. This disclosure describes a novel nucleic acid sequence that, when expressed in a plant (e.g., rice), regulates grain weight and grain number. This disclosure also describes mutant plants and transgenic plants.

The invention belongs to the field of plant biotechnology breeding, and particularly relates to a fine positioning molecular marker closely linked with rape grain weight main effect QTL-qSW.A3 and application of the fine positioning molecular marker. According to the present invention, the rape grain weight QTL-qSW.A3 is finely positioned through the molecular marker, the PARMS marker is adopted to transform and identify the genotype, and the QTL-qSW.A3 explains the average 10% phenotypic variance, and can be used for the genetic improvement of the rape grain weight and the yield;

The invention relates to application of rice genes SPPL1 and / or SPPL2 in improving high-temperature resistance of rice. Specifically, rice genes SPPL1 and / or SPPL2 participating in positive regulation and control of high-temperature stress resistance are cloned in rice, so that the rice genes SPPL1 and / or SPPL2 are overexpressed, and compared with a wild plant, the rice gene SPPL1 and / or SPPL2 overexpressed plant shows high-temperature-resistant characteristics, and agronomic characters (such as maturing rate, thousand seed weight, single-plant yield, plot yield and the like) are remarkably improved. Through cloning and discovery of new functions of the rice gene SPPL1 and / or SPPL2, a new gene target and a new resource are provided for improving high-temperature resistance genetic breeding of rice.

The invention discloses application of a sorghum SbMyb1 gene to regulation and control of grain yield, tannin and anthocyanin, and relates to the field of plant genetic engineering. The invention specifically discloses an application of a SbMyb1 gene or a related biological material thereof in regulation and control of plant economic traits. Experiments prove that the SbMyb1 gene encoding the protein with the amino acid sequence shown as SEQ ID NO: 2 can negatively regulate and control yield-related traits such as grain length and grain weight and positively regulate and control the content of secondary metabolites such as grain tannin and anthocyanin, has a good application prospect in high-yield, high-quality and stress-resistant molecular design breeding, and has a wide application prospect. And a new research thought is provided for the SbMyb1 gene to participate in the grain development process through the function exertion of the MBW complex.

The invention discloses a KASP molecular marker primer combination for identifying soybean hundred-grain weight character and application of the KASP molecular marker primer combination, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker assisted breeding. The invention discloses a KASP molecular marker primer combination for identifying the soybean hundred-grain weight character. The sequence of the KASP molecular marker primer combination is shown as SEQ ID NO.3-5. The invention provides a novel and simple molecular marker and an auxiliary selection method through the obtained KASP marker for regulating the hundred-grain weight character gene of the soybean, and is suitable for rapid screening of the relative height of the hundred-grain weight of the soybean and molecular marker auxiliary breeding. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The invention discloses a method for accurately identifying sweet-waxy corn germplasm, and particularly relates to a method for identifying sweet-waxy corn germplasm, which comprises a first tracking process: a process from F3-generation seed budding to F4-generation seed maturation, including sweet-waxy gene DNA tracking marking after seed budding; sampling once 28 days after pollination, and measuring the thickness of soluble sugar, amylose, amylopectin and pericarp; extracting DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) of F3-generation plants in a After the F4-generation seeds are mature and picked, determining sweet-waxy gene DNA, determining amylose and amylopectin, and determining single-plant grain weight, hundred-grain weight and endosperm / embryo dry weight; a second tracking process: a process from germination of the F4-generation seeds to bearing of fresh sweet-waxy corn, including observation and recording of a field corn plant growth process; measuring the thickness of soluble sugar, amylopectin, amylose and pericarp 15 days after pollination; determining and analyzing the traits of the indoor test species; data analysis and germplasm scoring: comparing and analyzing the data obtained in the second tracking process, scoring the F4-generation seeds, and determining the germplasm quality of the F4-generation seeds.