48 results about "Grain weight" patented technology

The application discloses protein TaANT1-4D and application of biological materials related to the protein in regulation of ear type traits of plants, and aims to solve the technical problem of how to regulate the ear type traits of plants.The amino acid sequence of the protein TaANT1-4D provided by the application is shown in sequence 2 in the sequence listing.Experiments prove that knocking out TaANT1-4D in a wheat variety Fielder can significantly shorten the ear length, reduce the number of spikelets, the grain length and the thousand-grain weight.Therefore, the protein TaANT1-4D has important application value in regulation of the ear type traits of wheat and has a broad prospect in cultivation of wheat varieties.

This invention discloses a breeding method for improving japonica rice by cultivating polymorphic lines. This invention integrates the GS3 long-grain gene, PigmR disease-resistant gene, fgr aroma gene, ALS herbicide-resistant gene, and COLD1 cold-tolerant gene into japonica rice. The GS3 long-grain trait successfully compensates for the reduction in thousand-grain weight caused by the PigmR gene. Simultaneously, the COLD1 cold-tolerant genotype repairs the genetic defect of low-temperature intolerance in the GS3 donor parent. Furthermore, it demonstrates the heat-resistant function of GS3 in the context of polymorphic lines, simultaneously improving grain shape, blast resistance, aroma, herbicide resistance, heat tolerance, and low-temperature tolerance. This provides new germplasm for the green and efficient production of japonica rice and has significant application value.

This invention relates to the field of agricultural technology, specifically to a mutant rice OsDSP1 gene and its applications. Compared to the wild-type rice OsDSP1 gene, the mutant OsDSP1 gene has a T base inserted after position 115 or a G base deleted at position 116. The mutant rice OsDSP1 gene provided by this invention can improve rice resistance to brown planthoppers, while also increasing grain length and thousand-grain weight, effectively increasing rice yield. This invention provides new gene resources and improvement targets for the breeding of insect-resistant rice varieties, and has significant application value for insect-resistant and yield-increasing plant breeding.

This invention discloses a haplotype molecular marker associated with thousand-grain weight in rice and its application, belonging to the field of plant molecular breeding technology. The haplotype molecular marker provided by this invention consists of multiple tightly linked SNPs, and its association with the "significantly increased thousand-grain weight" phenotype is extremely significant. P The value is <0.01), and it has good stability. By detecting this haplotype molecular marker, the thousand-grain weight performance of rice can be predicted quickly and accurately at the seedling stage without waiting for the grains to mature, which greatly improves the breeding efficiency. In addition, this haplotype molecular marker can be directly used for molecular marker-assisted breeding of high-yield or large-grain rice varieties, or it can be integrated into rice functional gene chips as a core marker, or used to develop low-cost PCR detection kits, which is highly practical. It provides a direct molecular tool for the genetic improvement of rice thousand-grain weight, which helps to achieve the targeted breeding of high-yield, large-grain rice varieties.

The present application relates to a method for increasing grain weight, and belongs to the field of molecular genetics. Two maize kernel type regulating genes are disclosed, and a method for increasing maize grain weight and yield by gene editing is disclosed.

This invention discloses US The application and methods of genes in regulating rice plant height, grain length, grain width, and grain weight belong to the field of crop genetics and breeding technology. This invention reveals for the first time the potassium ion channel protein gene. US This gene has a pleiotropic function in rice, regulating plant height, grain length, grain width, and grain weight. By silencing or knocking out this gene, rice plant height can be significantly reduced, lodging resistance can be enhanced, and grain length, grain width, and thousand-grain weight can be greatly increased. This overcomes the adverse effect of traditional dwarfing genes often being accompanied by smaller grains, achieving synergistic improvement in dwarfing and grain weight. The discovery of this gene provides a brand-new gene resource and an efficient molecular breeding target for high-yield, high-quality, and lodging-resistant rice breeding, and has important theoretical value and broad application prospects.

This invention discloses a molecular breeding method for creating new wheat germplasm highly resistant to Fusarium head blight, stripe rust, and leaf rust. This method first develops newly located loci for grain weight / grain length / grain width. QTGW / KL / KW-1A The linkage marker KASP.QTGW / KL / KW-1A was identified and its effectiveness was verified. A major active site for resistance to Fusarium head blight was also developed. QFhb-2DL The linkage markers Fhb-2D-dcaps for the candidate genes were used. During the breeding process, molecular marker selection was first used to ensure that the created DH lines carried... QFhb-2DL Candidate genes for resistance to Fusarium head blight and QTGW / KL / KW-1A The enhanced allelic variation ensures the basic resistance and yield traits of the bred germplasm or materials. Furthermore, after propagation of the DH line, phenotypic identification was conducted in Yangzhou and Chengdu, simultaneously screening for resistance to Fusarium head blight, stripe rust, and leaf rust. Using local yield control varieties as comparisons, this facilitates the selection of germplasm or varieties adapted to local conditions and resistant to both Fusarium head blight and stripe rust / leaf rust, achieving a breakthrough in disease-resistant wheat breeding in the middle and upper reaches of the Yangtze River.

Provided in the present application are a glucose 6-phosphate isomerase OsPGIp1 protein and an encoding gene thereof, and use of the protein and a substance for regulating the expression of the encoding gene of the protein in regulating the height, grain weight, effective tiller number, seed-setting rate, or yield of a plant. The rice gene OsPGIp1 can be used for rice genetic breeding, germplasm resource screening, and mechanism research on breeding.

This application belongs to the field of biotechnology and provides a rice transcription factor gene. OsNAC15 Or the application of its encoded protein in regulating rice disease resistance, said rice transcription factor gene OsNAC15 The nucleotide sequence is shown in SEQ ID NO:1; the amino acid sequence of the encoded protein is shown in SEQ ID NO:2. This invention confirms the transgenic function through verification. OsNAC15 It is a positive regulator of rice blast resistance, and overexpression OsNAC15 It can significantly enhance rice's resistance to rice blast (leaf blast), while knocking out this gene leads to a decrease in rice resistance. Moreover, the regulation of this gene does not affect agronomic traits such as plant height, number of panicles, number of grains per panicle, seed setting rate, thousand-grain weight, or rice quality. It provides an ideal gene resource for breeding disease-resistant, high-quality, and high-yield rice varieties.

The application discloses mining of a wheat kernel weight gene 5D genome sequence SNP and development and application of a KASP molecular marker. The application provides a method for identifying or assisting in identifying a wheat kernel trait, and comprises the following steps: detecting whether the genotype of a SNP226 site in the genomic DNA of a wheat to be detected is an AC genotype, a GG genotype or a CG genotype; the kernel trait of a CC genotype wheat is better than that of a GG genotype wheat. The application has a good application prospect in molecular marker assisted selection and molecular design breeding for improving the wheat kernel weight.

This invention discloses a method and application for heterologous overexpression of the ZjUBQ3 gene from jujube trees to increase grain length and thousand-grain weight in rice, relating to the field of plant genetic engineering. The coding sequence of the ZjUBQ3 gene was obtained from the jujube variety 'Dongzao' via PCR amplification. This sequence is 1146 bp in length, encoding 381 amino acids composed of five ubiquitin monomers linked together, and its nucleotide sequence is shown in SEQ ID NO:1. This sequence was constructed into an overexpression vector and transformed into the rice variety 'Zhonghua 11', achieving heterologous overexpression of ZjUBQ3 in rice. Experimental results showed that, compared with the wild type, the transgenic rice lines heterologously overexpressing ZjUBQ3 exhibited significantly increased grain length and thousand-grain weight, while grain width remained largely unchanged. This invention confirms that the ZjUBQ3 gene from jujube trees has a positive regulatory effect on seed size, providing a valuable new gene resource for molecular breeding of high-yield and high-quality rice.

The present application belongs to the field of wheat growth regulation, and particularly relates to a wheat growth regulation method. In view of the technical problem of negative correlation between the growth cycle and yield of wheat, a new method for cultivating wheat is provided. The present application sprays putrescine on the wheat ear, which can effectively shorten the growth cycle of wheat while ensuring the stability of effective tiller number, thousand-grain weight and ear grain number, and avoiding yield loss. The mechanism of action of putrescine may match the endogenous putrescine metabolism characteristics of wheat, realize the synergy of growth regulation and yield maintenance, and effectively improve the protein content.

This invention discloses AP2 Genes or their encoded proteins and their uses in increasing plant yield or promoting plant growth. This invention relates to genes identified in maize. ZmAP2 The gene was overexpressed in rice, and compared with the control (CK), the overexpressing plants showed a significant increase in panicle grain number, grain length, thousand-grain weight, biomass, and plant height; this invention further uses its homologous rice OsAP2 Gene overexpression in rice ， Compared to the control (CK), the overexpressed rice plants showed a significant increase in the number of grains per panicle, significantly longer grains, significantly increased thousand-grain weight, and significantly higher yield per plant. This invention has potential applications in promoting crop growth, increasing crop yield, and breeding high-yield crop varieties.

The application discloses corn phosphatidyl phospholipase C4, a coding gene thereof and application of the corn phosphatidyl phospholipase C4 in enhancing disease resistance. The corn phosphatidyl phospholipase C4 can endow transgenic rice with wide resistance without affecting the yield of the rice. The amino acid sequence of the corn phosphatidyl phospholipase C4 is SEQ ID No.2. The sequence of the coding gene is SEQ ID No.1. Research shows that the corn phosphatidyl phospholipase C4 gene is located in the cell membrane, overexpression of the corn phosphatidyl phospholipase C4 gene in rice can improve the resistance of the rice to rice blast caused by Magnaporthe oryzae and rice bacterial leaf blight caused by Xanthomonas oryzae, and meanwhile, the agronomic characters of the rice are evaluated, and it is found that the gene does not affect the ear length, grain length, 1000-grain weight and yield per plant of the rice.

The application discloses a protein, a gene and application for regulating insoluble gluten content of wheat, and relates to the technical field of biology.The amino acid sequence of the protein is shown as SEQ ID NO.1.The nucleotide sequence of the gene is shown as SEQ ID NO.3.The gene IGC-7B is obtained through fine positioning and map-based cloning.The gene negatively regulates the insoluble gluten content of wheat.The gene IGC-7B is knocked out in wheat through CRISPR-Cas9 technology, and an EMS excellent variation mutant is created in a wheat cultivar to study the function, it is found that the insoluble gluten content of wheat is increased after the gene is mutated, and the grain weight and yield are increased, and the function is verified through haplotype analysis in an AM3 wheat natural population and a rice natural population.The gene IGC-7B can realize the synergistic improvement of grain quality and yield.

This invention discloses the application of the jujube ZjUVI4 gene in rice grain shape improvement, belonging to the field of plant genetic engineering technology. The invention cloned the coding region sequence of ZjUVI4 from the jujube variety 'Dongzao' using PCR. The full-length sequence is 774 bp, encoding 257 amino acids. The nucleotide sequence of the coding region of the ZjUVI4 gene is shown in SEQ ID NO:1. Then, an overexpression vector was constructed, and transgenic experiments were conducted using the rice variety 'Zhonghua 11' as the transformation recipient. The ZjUVI4 gene was heterologously overexpressed in rice, and its biological function in the positive regulation of rice grain shape was investigated. This invention reveals the biological function of the jujube ZjUVI4 gene in positively regulating seed size. By transforming the ZjUVI4 gene into rice, the transgenic rice lines showed a significant increase in grain length and thousand-grain weight, while grain width remained largely unchanged, providing a new gene resource for rice grain shape improvement and high-yield, high-quality breeding.

The application provides a soybean grain weight major QTL qSW20-1, a developed Indel molecular marker and application thereof, and belongs to the technical field of soybean molecular breeding. The QTL qSW20-1 provided by the application is located on the 20th chromosome of a soybean Glyma.Wm82.a4.v1 genome version, and the interval is reduced to between two SSR markers BARCSOYSSR_20_0830 and BARCSOYSSR_20_0842 by using a residual heterozygote. The same allele of the QTL qSW20-1 and large grain has a significant positive influence on the number of grains per plant and the grain weight per plant, but has no adverse influence on the protein content and the fat content of quality traits. Meanwhile, a molecular marker Indel67 related to the hundred-grain weight is developed, and can be used for molecular marker assisted selection breeding and improving the individual selection efficiency in a breeding population, and accelerating the breeding process.

This invention discloses a set of KASP primers related to wheat grain weight and their applications. The primer set includes primers with nucleotide sequences as shown in SEQ ID NO.7, SEQ ID NO.8, and SEQ ID NO.6. This KASP primer set can be used to detect loci on wheat chromosome 5D that are associated with thousand-grain weight. Ktkw-5D, Allele C / T typing was performed at this locus. Wheat carrying allele C had a significantly higher thousand-grain weight than wheat carrying allele T, thus enabling early screening of wheat grain weight and accelerating the breeding process.

The application belongs to the field of genetic engineering, and provides application of LOC_Os03g43100.2 gene and the protein coded by the gene in improving rice traits, and a method for cultivating transgenic rice with improved grain size and / or yield. The application obtains transgenic rice with grain length, grain width of matured rice, grain length, grain width of brown rice, and thousand-grain weight of the transgenic rice being obviously greater than those of wild-type rice, and the improvement effect on the traits of rice grains is very obvious, and the application is helpful to clarify the relationship between grain type and rice yield, and lays a theoretical foundation for cultivating high-yield rice varieties.

Some combine harvesters determine a decrease in turning accuracy based on the number of retries for the turning path and the weight of grain stored in the grain tank, and if a decrease in turning accuracy is detected, they perform a grain discharge operation from the grain tank. However, this only determines that turning accuracy decreases when the weight of grain stored in the tank increases, and does not improve turning accuracy. Therefore, we provide a combine harvester that improves turning accuracy by automatically adjusting the turning force, thereby reducing the operational burden on the operator to improve turning accuracy and improving work efficiency. [Solution] In a combine harvester equipped with a chassis 2 on which left and right travel devices 4L and 4R are mounted, a threshing device 6 and a grain tank 7 for storing grain are provided, and a harvesting device 15 is provided at the front, the control device 20 automatically changes the braking force of the brakes according to the amount of grain stored in the grain tank 7 detected by the yield sensor 30.

This invention discloses the application of the protein OsEAR9 in regulating rice plant height and yield. The amino acid sequence of the protein OsEAR9 is shown in SEQ ID NO:1. Experiments have shown that overexpression of the OsEAR9 gene in rice yields transgenic rice; this transgenic rice exhibits significantly increased plant height, panicle length, number of primary branches, number of secondary branches, number of grains per panicle, and yield per plant. Inhibition of the OsEAR9 gene in rice yields OsEAR9 knockout rice, i.e., OsEAR9 homozygous mutants; the OsEAR9 homozygous mutants show significantly reduced plant height, panicle length, number of secondary branches, number of grains per panicle, and thousand-grain weight, but do not affect yield per plant. Therefore, the protein OsEAR9 can regulate rice plant height, yield, and panicle shape. This invention has significant application value.

This invention belongs to the field of molecular biology and discloses the major QTL of maize kernel protein. qHP3.7 This invention provides the acquisition of the protein, the development and application of its molecular marker primers, and related technologies. It also provides a major-effect QTL for controlling maize kernel protein. qHP3.7 Located on the third chromosome of maize, this locus is a major QTL site controlling variations in maize kernel protein. A closely linked superior haplotype SNP marker, located at nucleotide 183,842,554 on the third chromosome of the maize B73 reference genome, explains 10.23% of the maize kernel protein phenotypic contribution. PARMS markers designed using this SNP were used to detect variations in maize inbred lines, demonstrating ease of operation, clear genotyping, and good selection effects on single ear grain weight and 100-grain weight yield traits. This marker can be used for marker-assisted selection breeding of maize kernel protein content.

The application provides a CAPS-153 marker and a detection method related to wheat starch content and grain weight, and belongs to the technical field of genotyping. The application provides a sequence of a SNP site closely linked to the thousand-grain weight trait of wheat grains, and provides three SNPs sites on a precursor sequence of tae-miR1121-2A, and five SNPs sites in a promoter region, and the eight SNPs can be closely linked to form two haplotypes Hap1 and Hap2 related to the thousand-grain weight. The application develops a CAPS functional marker to distinguish the wheat haplotypes Hap1 and Hap2, and Hap2 is an excellent haplotype. Therefore, the CAPS functional marker can distinguish the two haplotype wheat varieties of tae-miR1121-2A, and can be applied to molecular marker assisted selection breeding of new wheat varieties with high starch content in the future.

This invention belongs to the field of molecular biology and discloses the main QTL for maize yield tolerance to waterlogging. qWTCKNR3.2 This invention provides the acquisition of [specific compound name], the development and application of its molecular marker primers, and the main effect QTL for controlling the waterlogging tolerance coefficient of maize rows. qWTCKNR3.2 Located at nucleotide 218456862 on chromosome 3 of the maize B73 reference genome, this SNP contributed 14.53% to the phenotypic contribution of row number to waterlogging tolerance. PARMS markers designed using this SNP were used to test maize inbred lines, revealing that the method is simple to operate and produces clear genotypes. Furthermore, it demonstrated good selection effects on traits such as plant height, ear position, number of tassel branches, tassel main axis length, leaf length, number of ear rows, ear weight, and single ear grain weight under waterlogging stress. Therefore, it can be used for marker-assisted selection breeding of maize yield and waterlogging tolerance traits.

The application belongs to the field of rice genetic breeding, and discloses a rice SBEIIb mutant protein and application thereof in changing starch crystal structure and increasing resistant starch content. The mutant protein simultaneously contains double-amino-acid substitution (M610I / D611N) of substitution of M at the 610th position to I and substitution of D at the 611th position to N. The mutant protein can change rice starch from A-type to C B -type, significantly increase amylose content from 13.8% of the wild type to 20.3%, and significantly increase starch gelatinization temperature. Meanwhile, the mutant protein (M610I / D611N) can increase the resistant starch content in rice by about 5 times; compared with the ae mutant with SBEIIb deletion, the grain width, grain thickness and grain weight are significantly increased, and the chalky kernel rate, chalky area and chalkiness are significantly reduced. The SBEIIb-M610I / D611N in the application has important application value in cultivating functional rice varieties rich in resistant starch.

The application provides an amino acid application method for improving wheat grain weight and drought resistance, and belongs to the field of agricultural technology.The method mainly sprays glutamic acid solution at the wheat heading stage and the flowering stage, and the method can significantly improve the drought resistance of plants and offspring wheat at the grain filling stage.The results of examples show that the method can improve the SPAD value, photosynthetic rate and antioxidant enzyme activity of wheat under post-flowering drought stress, prolong the grain filling duration of wheat, and finally increase the dry matter accumulation amount, total starch content and grain weight.Meanwhile, the method can also maintain the germination energy, alpha-amylase activity, soluble sugar content, plant height, root number, total root surface area, total root volume of offspring wheat under PEG-6000 stress, reduce the relative permeability of the cell membrane, reduce the harm of drought stress to offspring wheat, and finally show that the adaptability of offspring wheat to drought stress is enhanced.

The application belongs to the technical field of biology, and particularly relates to a functional site of a maize gene KW6 and an application thereof. The application provides the maize gene KW6 which is applied in any one of the following: B1) regulating grain weight of a plant; B2) regulating yield of a plant; B3) plant breeding; experiments prove that KW6 the maize gene regulates grain weight or / and yield of a plant, and a knockout strain obtained by knocking out KW6 the maize gene has a significantly increased grain weight and yield compared with a wild type LH244. Therefore, it is proved that KW6 the maize gene and the encoded protein thereof negatively regulate grain weight and yield of a plant, and editing KW6 the maize gene by using a gene editing technology has a great application potential in molecular breeding.