613 results about "Grain weight" patented technology

The invention discloses application of gene miR408 and UCL (uclacyanin) in cultivating high-yielding rice. By analyzing miRNA in charge of specific expression in rice grains and embryos, it is found that the gene miR408 has an important regulating effect in rice grain growth, and the function is achieved by lowering the target gene UCL of the gene miR408. According to the application, rice mutant strains over-expressing miR408 and UCL-RNA interference and UCL-CRISPR knockout are respectively constructed. The research shows that the three types of mutant strains all have the phenomenon of rapid growth, and the drooping phenomenon of rice ears at the maturation stage is more obvious compared with that of a wild type; in addition, the fruiting rate is increased, ears are larger in size, the number of grains per ear is much larger compared with the wild type, and the thousand grain weight and the yield are also remarkably increased. According to the application, a brand-new, simple and effective method is provided for cultivation of high-yielding rice and can be applied and popularized on a large scale; besides, the application will not pollute the environment, and the rice is safe to eat.

The invention belongs to the field of microbial resources, and particularly discloses a strain of Bacillus aryabhattai J5 and application thereof in promoting growth of corn. The Bacillus aryabhattai J5 is collected in CGMCC (China General Microbiological Culture Collection Center) on October 15th, 2015 and is filed with the number of CGMCC NO.11508. The Bacillus aryabhattai J5 has functions of producing auxin, siderophore, ammonia and protease, dissolving phosphorus, resisting to salt, realizing antagonism, and the like. As the strain is capable of growing normally in a culture medium with salt content equal to 11%, the strain has good potential in eliminating soil salinization. As content of siderophore produced by the strain is up to 53.7%, the strain has effective disease-prevention property. By the strain, corn seed germination rate can be increased by 12.5%, and accordingly the strain has good growth promoting effect. By application of microbial inoculum prepared from the strain, corn yield is increased by 37.1%, and hundred-grain weight is increased by 35%. It is possible to develop the strain into microbial inoculum, and accordingly the strain has wide application prospect in terms of improving soil nutrient, eliminating salinization, promoting growth of plants, preventing diseases and the like.

The invention relates to a weighing machine and a weighing method, in particular to a quantitative packaging scale for sub-package according to amount of grains and a quantitative weighing method of the quantitative packaging scale. The packaging scale comprises a storage hopper, a unit grain weight acquiring unit and a quantitative packaging part, wherein the unit grain weight acquiring unit comprises a sampling device, a feeder, a plurality of lines of vibrators, a storage chute, a camera, a camera controller, a light source and an input device; the quantitative packaging part is a single-head quantitative packaging scale or a multi-head quantitative packaging scale, and comprises a material distribution chute, a feeding device, a weighing hopper and a weighing controller; and both the operation of the unit grain weight acquiring unit and that of the quantitative packaging part are coordinated and controlled by a PLC (Programmable Logic Controller). According to the invention, the unit grain weight of a material can be acquired instantly, so as to correct the sub-package weight and improve the precision of sub-package according to amount of grains.

The invention relates to a granary, a stored grain weight detection system and method and a sensor arrangement method, and provides the granary stored grain weight detection method based on the arrangement of two loops of sensors arranged on the bottom. The core technology comprises arrangement of inner and outer two loops of bottom pressure sensors, grain pile weight detection model based on bottom pressure intensity and side pressure intensity mean value estimation, and system calibration and modeling method and the like. The method has the advantages of high detection precision, low requirement for the performance of sensors, high applicability, simple device, convenient use and maintenance, and facilitating to carry out remote online granary quantity detection and granary condition monitoring and the like, and the demand for carrying out remote online quantity detection of grains stored in commonly-used granaries can be met. Since the detection method can be suitable for quantity detection of stored grains in a plurality of granary structures, the method has huge application value, and provides a new technological means for guaranteeing nation grain quantity safety.

The invention belongs to the field of rape molecular breeding, and relates to preparation of specific molecular markers of related genes MINI3 and TTG2 of the brassica napus grain weight. Double haploid colony (DH) is constructed with brassica napus I A 254 as a female parent and a brassica napus I A 177 as a male parent through hybridization, and the DH colony genotype and the thousand seed weight data are analyzed to obtain a QTLs locus of grain weight character. The MINI3 and the TTG2 genes of the IA254 and the IA177 are cloned by using a homology based candidate gene method, specific molecular markers MINI3a and TTG2a of the MINI3 and the TTG2 genes are designed according to sequence different locuses, and the molecular markers MINI3a and TTG2a are located on two grain weight QTLs locus of an A5 linkage colony for related verification and application, which proves that the molecular marker prepared by the invention is a novel genetic marker. The gene sequence is obtained firstly. The invention provides a novel marker for the molecular breeding of the brassica napus grain weight, and also provides useful information for candidate gene clone and marker auxiliary selection of thethousand seed weight character locuses of the brassica napus.

A field management method of high-end kiwi fruit during a fruiting period relates to a cultivation management method which is applied after a kiwi fruit plant can fruit. By systematic treatment of the method comprising application of fertilizer prior to flowering, bud picking, pollination, fruit thinning, application of fruit expanding fertilizer, application of leaf surface fertilizer, pest control, bagging, core picking, drought resistance, drainage of stagnant water, base fertilizer application, winter pruning, winter orchard-clearing disinfection and the like, the kiwi fruit can fully and efficiently utilize light, temperature, water, air and fertilizer, the relations of branch and leaf growth and fruiting are adjusted in real time, distribution of plant nutrient is adjusted, the fruiting capability of the kiwi fruit can be brought into play as far as possible, single-fruit grain weight and the number of contained seeds are increased, pest and disease damage can be eliminated simultaneously, and the disease-resistant capability of plants is enhanced. Besides, the trophic structure of the soil can be adjusted, soil fertility is not reduced all the year round, high quality, high yield and stable yield of the kiwi fruit are achieved, and the age limit of fruiting is prolonged.

The invention discloses a gene GW8 (Grain Width in Chromosome 8) (SEQ ID NO:1-6) for controlling rice grain width, grain weight and yield and an application thereof. The GW8 can control the shape and the size of a rice grain. The lateral and longitudinal division speed of the cell is affected by the GW8 to control the size and the length-width ratio of a husk, and the filling speed is regulated so as to affect rice yield. The GW8 can serve as a molecular marker for identifying the length-width ratio of the rice kernel and the kernel size species. The GW8 has a wide application prospect on breeding on the basis of improving rice yield and improving rice quality.

The invention relates to a grain bin stored-grain quantity detection method based on a structure self-adapting detection model. Two circles of pressure sensors are arranged on the bottom face of a grain bin and output values of the sensors are detected and according to the detection model (as is shown in the specification), the grain bin stored-grain weight estimation W<^> is calculated. The method has the characteristics of being high in detection precision and adaptive to stored-grain quantity detection of a plurality of kinds of grain bin structure types and the like.

The invention discloses a potato circulating production process by means of a detoxified potato test tube. The potato circulating production process comprises the following steps of test-tube strong seedling cultivation and test-tub potato induction. After the step of the test-tube strong seedling cultivation is performed, terminal buds of detoxified potato test-tube strong seedlings cultured in the step of the test-tube strong seedling cultivation are sheared, and then the detoxified potato test-tube strong seedlings without the terminal buds are added into a test-tub potato induction culture medium to perform test-tub potato induction production. Sheared terminal buds are continuously used for the test-tube strong seedling cultivation. The potato circulating production process is optimized while improving the production quantity per unit area and grain weight of test-tub potatoes, shortens production procedures, reduces the production cost, achieves annual reciprocating circulation to promote the detoxified potato production by means of the detoxified potato test tube, accelerates breeding, popularization and application of the detoxified potatoes, and accordingly promotes improvement of the production level of potatoes in our country and quick development of potato industry.

The invention relates to a ridge-covering and trench-sowing cultivation and fertilization technology of dryland winter wheat, which comprises the following steps of: preparing land, applying a base fertilizer, forming ridges with the widths of 35-40cm and covering a white plastic film on the ridges; preserving a ridge width of 30-35cm between two ridges; sowing wheat in grooves with a row distance of 15-20cm and a rowing amount of 150-225 kg/ha; uncovering the film during the total growth fertility period of the wheat so as to keep the strip film covering the soil surface; ditching and topdressing a nitrogen fertilizer of 30-45 kg/hm between wheat rows when the soil starts to defrost in spring and the wheat starts to turn green; milling in time after topdressing the fertilizer; keeping the plastic film covering the ridges after wheat is harvested; and returning all straws to be covered in grooves among the ridges for stimulating the rainfall infiltration in summer and lessening the water evaporation loss of the soil. The invention can stimulate the formation and the transferring of dry substances to grains, remarkably improves the ear grain number and the thousand grain weight and remarkably increases the wheat yield.

The invention discloses a primer pair for assaying the thousand-grain weight of wheat and a related molecular marker. The specific primer pair disclosed by the invention consists of a single-stranded DNA fragment shown in a sequence 3 of a sequence table and a single-stranded DNA fragment shown in a sequence 4 of the sequence table. The invention discloses allelic variation sequences, namely TaGS-D1a and TaGS-D1b, of an ordinary wheat gene TaGS and provides functional markers for assaying the allelic variation of the TaGS-D1a and the TaGS-D1b and the relationship between the functional markers and the thousand-grain weight of the wheat. Through applying the functional markers provided by the invention to molecular-marker-assisted selection, wheat varieties (materials) with higher thousand-grain weight can be quickly screened out, and thus, the pace for breeding new high-yielding wheat varieties is accelerated. The primer pair and the related molecular marker have important theoretical significance and economic value in the molecular-marker-assisted selection of the high-yielding wheat varieties.

The invention relates to a wheat cultivating method which comprises the following steps: selecting a land parcel with yield per mu of at least 500 Kg and organic matter weight content larger than 1 percent; fertilizing by adopting a prescription by the weight ratio of nitrogen to phosphorus to potassium of 3:1;4; selecting a wheat variety with good plant type structure, straight and big plant, more grains, high photosynthetic efficiency and grouting speed and stable grain weight; sowing the wheat variety twice in ways of line space shortening, sowing amplitude widening and planting density increase; and carrying out management before winter, spring management and later period management in sequence. The wheat cultivating method breaks through a normal mode of high water, fertilizer and sowing contents in the prior art, effectively solves the gradually raising contradictions between high yield and quality, high yield and lodging resistance, high yield and stable yield as well as high yield and efficiency, enables the wheat yield per mu to reach 700 Kg and has the advantages of simple technology and easy mastering.

The invention discloses a breeding method of high-quality double-low disease-resistant high-yield lodging-resistant rape cultivar 'Zhen 2405'. The bred high-quality double-low rape cultivar has the advantages of full and round seed grain, heavy thousand grain weight, black seed grain, good marketability, excellent disease resistance, stable yield, good lodging resistance, good plant-type trait, good maturity performance, consistent maturity and suitability for the mechanized harvest. The method comprises the following steps: A) selecting the early-mature high-yield rapeseed line 'Zhen 9701' as the maternal plant, which has high resistance to Sclerotinia sclerotiorum and lodging and has compact plant-type trait, selecting rape line F1 as the male parent, which has good agronomic characters and high-yield trait, and cross-breeding to obtain a rape line F0; and B) sowing the rape line F0, sowing the excellent individual plants after the two-row sowing of the rape line F0, and self-inbreeding for 6 generations to obtain the high-quality double-low high-yield rape cultivar (Brassica napus) 'Zhen 2405' with excellent resistance to Sclerotinia sclerotiorum and lodging.

The invention relates to the technical field of plant genetic engineering and discloses clone and application of semi-dominant gene qGL3 capable of controlling grain length and grain weight of rice kernel. In the invention, a main-effective QTL (quantitative trait loci) SEQ ID NO:1 which is capable of simultaneously controlling the grain length and grain weight of rice kernel and a semi-dominant allelic gene SEQ ID NO:3 having advantageous function are separated and cloned; the two genes have the capacities of modifying the yield and appearance quality of rice; and at the same time, the two homologous genes of the two genes in rice are cloned and a fact that the two homologous genes have regulation effect on the length of rice kernel as well is proved. Thus, the gene qGL3 and the advantageous allelic gene qGL3-D thereof as well as the two homologous genes of the gene qGL3 all can be applied to crop genetic modification.

The invention relates to a lodging and senility resisting, high photosynthetic efficiency and yield increasing regulator for corn, and a preparation method and an application thereof. The regulator contains 5-aminolevulinic acid, 2-(3,4-dichlorophenoxy)triethylamine and ethephon, and preferably contains an activator and a spreader, and the activator and the spreader are selected from one or more of tween20 and tween60. The regulator can substantially enhance the low temperature cold injury, high temperature, drought and low light stress resistance of corn, increases the effective spike number, the number of grains per spike and the thousand-grain weight of the corn, and increases the output of the corn by 20% or above. The regulator has the characteristics of low cost, use convenience and less field residual, is easy to promote and apply, and has an active promotion effect on Chinese corn production.

The invention discloses a rice overgrowth-controlling, lodging-resistant and yield-increasing agent and an application thereof. The rice overgrowth-controlling, lodging-resistant and yield-increasing agent mainly comprises a plant growth regulator and a fertilizer, wherein the regulator is brassinolide and paclobutrazol or uniconazol, and the fertilizer is one or combination of more than two of substances containing N/P/K/B/Zn/Ca/Cu/Fe/Mn/Mo/Me/S/Cl/Si and other elements, such as urea, potassium dihydrogen phosphate, diammonium phosphate, calcium carbonate, potassium nitrate, silicon fertilizer, zinc sulfate, ferrous sulfate, potassium chloride, boric acid, borax, copper sulfate, ferrous sulfate, molybdenum fertilizer, magnesium sulfate, potassium sulfate, manganese sulfate and rare earth fertilizer. The rice overgrowth-controlling, lodging-resistant and yield-increasing agent has good safety and can effectively shorten the length between neck-panicle nodes and top second nodes of rice and enhance lodging resistance of rice; simultaneously, the rice overgrowth-controlling, lodging-resistant and yield-increasing agent can not affect safe dates of full heading and the grain number per spike of rice, prolong the service life of functional leaf of rice, improve the seed setting rate and the 1000-grain weight of rice and significantly affect the yield of rice. The rice overgrowth-controlling, lodging-resistant and yield-increasing agent is suitable for various types of rice, including conventional rice, hybrid rice and super hybrid rice, and has the advantages of low cost and high performance-price ratio.

The invention relates to a planting method for wild ginseng in the forest. The method comprises the following steps: A, land selection: selecting mountain land podzolic soil as the planting soil; setting the altitude between 500m and 800m and the slope between 15 and 30 DEG; selecting a conifer broadleaf mixed forest as the vegetation; and selecting a temperate zone mountain conifer broadleaf forest climatic type as the climate; B, the forced sprouting for the seeds: selecting secondary horse tooth ginsengs or round upper arm and round rhizome-shaped ginsengs as seeds; C, seeding: dividing the seeding into spring seeding or autumn seeding, pricking holes with the depth of between 4 and 5cm and the hole space of 20cm, and sowing 1 to 2 seeds in each hole; and D, reserving seeds for planting and harvesting seeds: carrying out the seed reservation and seed selection for the 8-year wild ginseng in the forest annually, wherein the step D comprises the following steps: (1) seed reservation: reserving the seeds for planting annually, and thinning flower and fruits; and (2) seed harvest: harvesting the fruits when the fruits are mature and red, cleaning the fruits with water, and achieving the thousand grain weight of the seeds above 30g. In the cycle of the annual seed picking, seed selection, sprouting, seeding, seed reservation, seed harvesting and comprehensive purification and optimization for the wild ginseng in the forest, the wild ginseng in the forest with combined good shape and quality is cultured and finally reaches the performance and index of the wild mountain ginseng.

The invention discloses a molecular marker closely linked with a major quantitative trait locus (QTL) of the thousand-grain weight and grain length of wheat and an obtaining method and application of the molecular marker. A labeling primer wmc386 and barc216 are adopted to carry out polymerase chain reaction (PCR) amplification on the DNA of a wheat line Shannong 0431; and after the electrophoretic separation of the amplification product on 8% polyacrylamide gel, the molecular weights of the obtained amplification product are 190 bp and 100 bp respectively, i.e., the molecular marker of the major QTL of the thousand-grain weight and the grain length of the wheat. The molecular marker of the major QTL of the thousand-grain weight and the grain length of the wheat can be used for detecting whether the QTL for increasing the thousand-grain weight and the grain length is contained in a wheat variety or a wheat line, in order to fast screen out the wheat variety or the wheat line containing the QTL for increasing the thousand-grain weight and the grain length for breading; therefore, the selective breeding process for high-yield wheat varieties is much faster.

The invention discloses a method and a device for performing high-precision determination of corn ear variety based on images. A corn ear week view using cob as central axis is established and the week view is processed by image processing algorithms to obtain data of morphological index, ear grain row number and per row grain number, and the total grain number of ear grains is calculated based on the ear grain row numbers and the per row grain numbers; the corn ears are threshed to produce a cob and ear grains; the total weight of the ear grains or the weight of the cob is measured, and the total weight of the ear grains is calculated based on the weight of the corn ear and the weight of the cob; and the mean grain weight and the 100-grain weight are calculated based on the total weight of the ear grains and the total number of the ear grains. Before thresh of the corn ears, the weight of the corn ears is measured by a weighing sensor. With the digital image processing and sensor techniques, after obtaining the one-week ear grain distribution diagram of the corn ears, and the parameters of all the corn ear grains are calculated, including morphological index, ear grain row number, per row grain number, mean grain weight and so on. The precision of determining the variety of the corn ears is improved.

The invention discloses molecular markers closely linked with multiple-effect major QTL of the grain weight or silique length of rape, and application thereof. The closely-linked molecular markers canbe used for molecular marker-assisted selection of rape and map-based cloning of the QTL. According to the invention, a hybrid F1 and parents are subjected to continuous backcrosses for multiple generations, and a hybrid near-isogenic line is constructed by cooperatively using a molecular marker-assisted selection method; individual plants with high background reversion rate are subjected to selfing so as to obtain a QTL-NIL segregation population; molecular markers are gradually encrypted in a target region so as to realize genotype analysis of the population and searching of crossover individual plants; and thus, the molecular markers BrSF7-101 and BrSF6-2389 are obtained, and the physical distance between the two molecular markers is only 134 kb. The two closely-linked molecular markers are used for genetic typing of linked populations, and it is found that two alleles are greatly different in grain weight and silique length; and thus, the molecular markers can be applied to molecular marker-assisted selection of rape, improve selection efficiency and accelerate breeding progress.

The present invention relates to a kind of special fertilizer for peanut. When the soil is alkaline, the total nutrient range of said special fertilizer is 30%-36%, and the weight percentage of its formula is N:P:K=6-8:10-12:14-16, and when the soil is acidic, the total nutrient range of said special fertilizer is 25-30%, and the weight percentage of its formula is N:P:K=10-12:6-7:9-10. Said special fertilizer can raise average unit yield of peanut by about 30-40%, and can raise its thousand-grain weight by about 10% and raise its oil content by 6-8%.

The invention discloses a multicomponent composite fertilizer special for peanuts, which belongs to a crop fertilizer. The multicomponent composite fertilizer special for peanuts comprises the following components: 35 percent of total nutrients including 16 percent of nitrogen, 10 percent of phosphorus and 9 percent potassium, more than or equal to 10 percent of medium and trace elements, 7-9 percent of organic matter and 2-5 percent of amino acid. The medium and trace elements comprise trace elements including molybdenum, zinc, manganese, boron and iron. The multicomponent composite fertilizer special for peanuts has the advantages of nutrient content assurance and reasonable proportioning balance. The multicomponent composite fertilizer special for peanuts can effectively improve the immunological competence of the peanuts, regulate endogenous hormone, inhibit the regeneration of insect eggs, activate the activity of root azotobacter, promote root development, quicken nutrient absorption, achieve the functions of strengthening seedlings, promoting growth and promoting flowering, prevent bacterial wilt, continuous cropping symptoms and leaf spots, also prevent excessive growth and take special effect on symptoms including mosaic disease, chlorotic disorder, flocculi, defoliation and the like. After applied, the multicomponent composite fertilizer special for peanuts can effectively improve the percentage of fertile fruits and the thousand grain weight, promote the mellow and full degrees of fruits and achieve remarkable effect of production increase.

The invention relates to application of rice transcription factor Os05g39950 gene. According to the application, a transcription factor activation sequence motif VP64 (four transcription factor activation sequence motifs VP16) and the rice transcription factor Os05g39950 gene are fused and built to obtain a forming transcription factor, and the forming transcription factor is converted into crops such as rice, so the rice grain property is improved, for example, the rice grain is obviously shortened, widened and thickened, and the grain weight is reduced. The important theoretical value is realized on the detail clarification of seed development mechanism regulation and control, and the rice grain form can be improved through a transgenosis measure, so the important significance is also realized in the production practice.

The invention discloses a method of harvesting white atractylodes rhizome seeds. According to the method, the clarity of the harvested atractylodes rhizome seeds can reach up to above 96% and the hundred-grain weight can reach up to above 2.1532g by determining the best seed harvesting period, harvesting, drying, kneading, removing fuzzes, winnowing, bagging and storing. Compared with the 1.7749g of the hundred-grain weight, the seed quality index can be significantly improved, the empty rate can be reduced, the problems that the seeds are bad in quality and are free from endurable storage can be caused by green and empty seeds due to premature in the prior art can be effectively solved, the harvesting quality of the atractylodes rhizome seeds can be improved, the quality guarantee can be provided for the planting, sowing and seedling raising of the atractylodes rhizome seeds, the waste of atractylodes rhizome seeds can be prevented, the seed quality and seed clarity can be improved by adopting a way of drying and then kneading, and the storage time and germination rate of the seeds can be improved by adding peppers and controlling the storage temperature in a refrigeration house; furthermore, the method has the characteristics of being simple in harvesting method and high in harvesting efficiency.

The invention belongs to the technical field of rape molecular breeding and preparation of molecular markers, and particularly relates to preparation of a brassica napus L. grain weight major QTLs locus specific molecular marker. The marker can be used for auxiliary selection of molecular markers, fine positioning of grain weight trait loci and based cloning in the improvement on brassica napus L. grain weight traits. The marker is characterized in that double haploid (DH) is constructed by hybridizing brassica napus L. first A254 (large-grain pure line materials) as a female parent and brassica napus L. first A177 (small-grain pure line materials) as a male parent, and the molecular marker which is tightly linked with the grain weight major QTLs is obtained by analyzing DH group genotypes and thousand grain weight data and is named I0509 and J0609, and is relatively verified and applied. A new genetic marker is provided for molecular breeding of rape grain weight and also useful information is provided for fine positioning of thousand grain weight trait loci of the brassica napus L. and based cloning of relative genes.

The invention discloses a variable-speed response capsule glue-dipping device and method. A glue-dipping groove is improved and comprises an inner groove and an outer groove; under the action of a stirring rod at the opening at the bottom of the inner groove, a glue liquid springs out from bottom to top at the bottom of the inner groove so as to overflow into the outer groove and then enters the bottom of the inner groove from the outer groove for turnover, and the process is performed in a circulating and reciprocating manner to achieve the stirring effect; a second motor driving the stirring rod to rotate is matched with the operation of a glue-dipping cam driving device to perform high-speed rotation or low-speed rotation under the action of a variable-frequency controller; when the glue-dipping operation is stopped, the high-speed rotation is performed, thereby quickly and uniformly stirring the glue liquid; and when the glue-dipping operation is started, the stirring rod rotates at low speed so as to enable the glue-dipping liquid level and the glue liquid in the glue-dipping groove to be basically in a static state. Thus, the uniformity of glue films formed by glue dipping is better, and produced capsules have small difference in natural grain weight.