305 results about "Grain weight" patented technology

The invention discloses application of TaMYB-7B and a coding gene thereof in regulation and control of wheat tiller number, and belongs to the technical field of gene engineering. The invention finds for the first time that the TaMYB-7B gene is a novel gene capable of simultaneously regulating and controlling the tillering number and grain traits of wheat. The TaMYB-7B gene is interfered, knocked out, silenced or mutated, so that the tiller number of a plant can be increased, the grain length is increased, the hundred-grain weight is increased, finally, the yield of a single wheat plant can be increased, and a foundation is laid for wheat yield improvement and character improvement.

Provided in the present invention are a Brassica napus drought-tolerant gene BnaC04ARL and the use thereof. The gene is expressed in a plant to improve drought tolerance of the plant. The gene comprises a nucleotide sequence selected from the group consisting of: a nucleotide sequence shown as SEQ ID NO: 1; and / or a nucleotide sequence encoding an amino acid sequence shown as SEQ ID NO: 2. The overexpression of the gene can improve the viability of Brassica napus under drought conditions, enhance root system development, and improve the thousand-grain weight, the grain yield per plant, the number of siliques per plant, dry weight per plant, silique length, etc.

The invention discloses a molecular marker related to thousand seed weight of wheat, a detection primer and application of the molecular marker and the detection primer, and belongs to the technical field of molecular marker-assisted breeding. The nucleotide sequence of the molecular marker TaDTX50-STS is as shown in SEQ ID NO. 1; basic groups from the 502 site to the 661 site of the sequence as shown in SEQ ID NO. 1 have insertion / deletion mutation. The InDel molecular marker related to the thousand seed weight character in a wheat genome is identified and named as TaDTX50-STS, and by detecting the polymorphism or genotype of the InDel molecular marker, the InDel molecular marker can be used for identifying or assisting in identifying the thousand seed weight character of wheat, assisting in breeding dominant varieties with high thousand seed weight of wheat and improving the wheat breeding efficiency.

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

The invention relates to the field of rice gene engineering, and discloses an application of a GSE3.1 protein and a coding gene thereof in regulating and controlling the grain length, the grain width and the grain weight of rice seeds. According to the invention, a GSE3.1 gene is edited based on a CRISPR / Cas9 technology, a japonica rice variety Zhonghua 11 (ZH11) is introduced by using an agrobacterium-mediated method, and a knockout mutant is obtained through screening. The homozygous knockout mutation of the GSE3.1 gene in the invention leads to reduction of the grain width and grain length of rice seeds and reduction of thousand grain weight. According to the invention, the constructed plant overexpression vector ProActin: GSE3.1 is expressed in wild type ZH11, so that compared with the wild type plant, the seed grain length and grain width of the transgenic plant are obviously increased, and the thousand grain weight is obviously increased. Therefore, the GSE3.1 gene and the encoding protein thereof can regulate and control the size and the weight of the rice seeds, and are of great significance to cultivation of high-yield rice varieties.

The invention discloses a corn ZmMYBR37 gene and an application of the corn ZmMYBR37 gene in regulating and controlling the size of corn kernels. The nucleotide sequence of the ZmMYBR37 gene is as shown in SEQ ID No: 1 in a sequence table. A mutant material with small grains and reduced hundred-grain weight is separated from a corn EMS mutant library, and a mutation site occurs on a function unknown gene ZmMYBR37, so that protein translation is terminated in advance. The information analysis finds that the gene coding protein has a conservative MYB structural domain, and the gene coding protein is speculated to belong to an MYB transcription factor family. Gene expression analysis finds that the gene is high in constitutive expression in corn and high in expression level in leaves and embryos. The corn material overexpressed with the ZmMYBR37 has the advantages that the grain size is increased, and the hundred-grain weight is obviously increased, so that the ZmMYBR37 has the potential of increasing the corn yield.

The invention provides application of an OsRTD1 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsRTD1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the OsRTD1 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of the OsRTD1 gene mutant plant osrtd1 under the drought stress of PEG induction and soil dehydration is remarkably improved, the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced, the relative water loss rate of dehydrated leaves is higher, and the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced. The total grain number, the solid grain number, the total grain weight and the like of the OsRTD1 gene mutant plant osrtd1 under the stress of field drought are obviously higher than those of a wild plant. The OsRTD1 gene is a potential candidate new target for regulating and controlling a rice drought resistance mechanism.

The invention belongs to the technical field of plant molecular biology, and discloses an application of a soybean GmSW10 gene in reducing soybean plant height, grain weight and grease content and / or increasing protein content. The CDS sequence of the soybean GmSW10 gene is as shown in SEQ ID NO: 1. Knockout of the soybean GmSW10 gene can directly influence the plant height, the grain weight, the oil content and the protein content of soybean seeds, after the soybean GmSW10 gene is knocked out, the weight of soybean seeds and hundred grains is remarkably reduced, the plant height is reduced, the protein content is increased, and the oil content is reduced, which indicates that the GmSW10 gene is a key gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean, and the GmSW10 gene can be used for preparing the soybean GmSW10 gene for regulating and controlling the plant height, the grain weight, the oil content and the protein content of the soybean. And a new genetic resource is provided for genetic improvement of soybeans.

The invention discloses a rape grain weight regulation gene NGAL3 based on whole genome screening and a breeding application thereof, based on the seed size and grain weight phenotypic value of a cabbage type rape germplasm resource, a candidate gene interval is positioned by using GWAS; the method comprises the following steps: selecting large-grain-weight and small-grain-weight extreme phenotypic materials, performing transcriptome sequencing in a critical period of seed development, and screening differential expression genes; and performing cross comparison on the GWAS candidate gene and the differential expression gene to obtain a key gene for jointly regulating and controlling the size and the grain weight of the seed, and performing functional verification on the key gene. The thousand seed weights of the created homozygous three mutant strains L1-sg1-1-4-5 and L2-sg1-4-8-16 are obviously increased by 46% and 29% compared with those of the wild type strains. The invention provides a new target for high-yield rape breeding, and non-transgenic high-grain-weight germplasm can be created through gene editing or beneficial allelic variation of NGAL3 is selected and enriched under the assistance of molecular markers.

The invention discloses application of a KASP molecular marker in identification or auxiliary identification of soybean hundred-grain weight character, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker auxiliary breeding. According to the application of the KASP molecular marker in identification or auxiliary identification of the soybean hundred-grain weight character, the genotype of the KASP molecular marker is G or A, and the KASP molecular marker is located at the 7703700bp position of the No.6 chromosome of a soybean genome Wm82. A2. V1. The obtained KASP marker for regulating and controlling the hundred-grain weight character gene of the soybean is suitable for rapid screening and molecular marker-assisted breeding of the relative height of the hundred-grain weight of the soybean. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

The invention relates to the field of plant growth regulators, in particular to a conditioner group for improving the grain quality and the rice quality of direct seeding rice and a preparation and use method thereof.The preparation method of a conditioner in the initial heading stage comprises the following steps that 1, 6-benayl aminopurine is dissolved in a diluted hydrochloric acid solution in a assisted mode, and a 6-benayl aminopurine solution is obtained; step 2, adding choline chloride into the solution obtained in the step 1, stirring to obtain a mixed solution, transferring the mixed solution into a volumetric flask, and adjusting the pH value; 3, abscisic acid powder is dissolved with absolute ethyl alcohol, volume metering is carried out after dissolution to form a stock solution, and then PH adjustment is carried out. Two conditioners are included and sprayed in the initial heading stage and the full heading stage respectively, glumous flower degeneration can be reduced, the photosynthetic production capacity of the upper three leaves after flowering can be improved, assimilation substances are promoted to be transferred to grains, especially the filling state of vulnerable grains is improved, and the yield of the seedlings is increased. Finally, the grain number, the maturing rate, the grain weight and the yield are improved, meanwhile, the head rice rate of rice can be improved, and the protein content is reduced, so that the palatability of rice is improved.

The invention discloses a trans-boundary synthetic flora (SMC) for preventing and treating wheat stem rot as well as preparation and application of the trans-boundary synthetic flora. The synthetic bacterial flora is prepared by compounding Trichoderma harzianum T19 with the preservation number of CCTCC (China Center for Type Culture Collection) No: M 20251896, bacillus subtilis BS-Z15 with the preservation number of CCTCC No: M 20251892, two strains of growth promoting bacillus PM9 and PM16, and two strains of auxiliary bacillus S8 and S32 according to a specific proportion. Compared with an existing single antagonistic bacterial agent, the synthetic flora disclosed by the invention has the advantages that the bacteriostasis rate of the synthetic flora on Fusarium pseudograminearum under a non-sterile soil condition can reach 70% or above, and the disease index is reduced by 70.01%; meanwhile, the plant height of wheat is increased by 28.63%, the root length is increased by 24.53%, the fresh weight is increased by 181.21%, the dry weight is increased by 184.64%, the thousand seed weight is increased by 10.39%, and the rhizosphere microbial community structure and the soil enzyme activity are remarkably improved. The method can be used for green prevention and control and yield increase of basal stem rot of wheat and other gramineous crops.

The invention belongs to the field of plant biotechnology breeding, and particularly relates to a fine positioning molecular marker closely linked with rape grain weight main effect QTL-qSW.A3 and application of the fine positioning molecular marker. According to the present invention, the rape grain weight QTL-qSW.A3 is finely positioned through the molecular marker, the PARMS marker is adopted to transform and identify the genotype, and the QTL-qSW.A3 explains the average 10% phenotypic variance, and can be used for the genetic improvement of the rape grain weight and the yield;

The invention relates to application of rice genes SPPL1 and / or SPPL2 in improving high-temperature resistance of rice. Specifically, rice genes SPPL1 and / or SPPL2 participating in positive regulation and control of high-temperature stress resistance are cloned in rice, so that the rice genes SPPL1 and / or SPPL2 are overexpressed, and compared with a wild plant, the rice gene SPPL1 and / or SPPL2 overexpressed plant shows high-temperature-resistant characteristics, and agronomic characters (such as maturing rate, thousand seed weight, single-plant yield, plot yield and the like) are remarkably improved. Through cloning and discovery of new functions of the rice gene SPPL1 and / or SPPL2, a new gene target and a new resource are provided for improving high-temperature resistance genetic breeding of rice.

The invention discloses a KASP molecular marker primer combination for identifying soybean hundred-grain weight character and application of the KASP molecular marker primer combination, and belongs to the technical field of soybean agronomic character molecular marker development and molecular marker assisted breeding. The invention discloses a KASP molecular marker primer combination for identifying the soybean hundred-grain weight character. The sequence of the KASP molecular marker primer combination is shown as SEQ ID NO.3-5. The invention provides a novel and simple molecular marker and an auxiliary selection method through the obtained KASP marker for regulating the hundred-grain weight character gene of the soybean, and is suitable for rapid screening of the relative height of the hundred-grain weight of the soybean and molecular marker auxiliary breeding. The molecular marker can detect genotypes of materials with different hundred-grain weights in natural populations, can also be used for molecular-assisted selective breeding of soybean materials with different hundred-grain weights, replaces large-scale phenotypic screening, reduces field workload, and greatly improves screening accuracy and efficiency.

This invention provides an automatic calibration system for harvester yield detection coefficients and a harvester itself. The automatic calibration system includes a grain fullness detection sensor, a yield sensor, and a controller. Both the grain fullness detection sensor and the yield sensor are connected to the controller. The controller determines when the harvester is in a full-grain state based on the detection data acquired by the grain fullness detection sensor. It then acquires the grain full volume, the current grain bulk density collected by a bulk density meter, and the current cumulative system yield collected by the yield sensor. The yield detection coefficient is calibrated based on the current grain bulk density, grain full volume, and current cumulative system yield. Utilizing the harvester's existing grain fullness detection sensor as an automatic calibration switch, combined with the grain bulk density parameter measured by the bulk density meter input into the system, the system obtains the grain weight at the time of the full-grain alarm and automatically corrects the current yield coefficient, reducing yield measurement errors. Calibration is simple, does not increase costs, reduces the need for weighing sensor calibration, and enhances reliability.

The invention discloses an application of a GmFBXL87a or a GmFBXL87b protein in regulation and control of the sizes of soybean seeds. Belongs to the technical field of soybean breeding. The invention aims to regulate and control the sizes of soybean seeds. The invention provides an application of a GmFBXL87a or a GmFBXL87b gene in regulation and control of soybean grain size and hundred-grain weight, a nucleotide sequence of the GmFBXL87a is as shown in SEQ ID NO.3, and a nucleotide sequence of the GmFBXL87b is as shown in SEQ ID NO.4. The invention further provides a preparation method of the gene. And a theoretical basis is provided for improving the yield of soybeans.

The invention discloses a molecular marker of a wheat TaREC2-6B gene and application of the molecular marker, and belongs to the technical field of crop seed selection and cultivation. The molecular marker is a KASP marker and is obtained by amplifying a KASP marker primer group (AllleFAM, AllleHEX and Common), after a fluorescence signal is analyzed by a KlusterCaller, haplotypes of the wheat TaREC2-6B gene are divided into TaREC2-6B-HapI and TaREC2-6B-HapII, and the haplotypes of the TaREC2-6B gene are relatively wide in flag leaf width, relatively large in thousand seed weight and relatively large in single plant yield, so that the haplotypes of the TaREC2-6B gene are excellent genotypes of the wheat TaREC2-6B gene. The method has the beneficial effect that a new tool is provided for wheat breeding.

The application discloses a wheat TaMADS15-D gene and application of a coded protein thereof, and belongs to the technical field of plant genetic engineering and crop genetic breeding. TaMADS15-D The application discloses that the wheat TaMADS15-D gene is first disclosed to be capable of regulating nitrogen utilization efficiency, grain size and yield of wheat. TaMADS15-D Overexpression of the gene can significantly improve sensitivity of the wheat to chlorate, improve nitrogen utilization efficiency of the wheat, and significantly increase grain length, grain width and 100-grain weight of the wheat. The gene is a key gene capable of simultaneously regulating nitrogen utilization efficiency and grain size, and application of the gene to wheat molecular design breeding has important significance for cultivating a new wheat variety with high yield and high nitrogen utilization efficiency.

The application discloses a method for screening wheat with different thousand-grain weights and ear phenotypes, and comprises the following steps: detecting whether the genotype of a wheat to be tested based on a TaNAC92-2A gene is genotype I or genotype II, the ear length, the number of grains per ear, the number of fertile spikelets and / or the number of spikelets per ear of the wheat with genotype I are greater than those of the wheat with genotype II; the thousand-grain weight of the wheat with genotype I is less than that of the wheat with genotype II; the genotype of the wheat with genotype I based on an A415G SNP site is AA homozygous type; the genotype of the wheat with genotype II based on the A415G SNP site is GG homozygous type; and the A415G SNP site is the 415th nucleotide from the 5' end in the wheat genome, as shown in SEQ ID NO: 1. By detecting the genotype of the wheat to be tested based on the TaNAC92-2A gene, the wheat thousand-grain weight and / or the ear phenotype can be screened. The application has important application value.

The invention discloses wheat TaPAB1 protein and application of related biological materials of the wheat TaPAB1 protein in the aspect of improving the grain weight of wheat. A gene for coding the TaPAB1 protein is named as a TaPAB1 gene. According to the invention, the gene TaPAB1 is cloned from a wheat cultivation variety, Chinese spring, and is over-expressed in a wheat variety Kenong 199 (KN199) by utilizing a gene engineering technology, so that the thousand seed weight, the grain length, the plant height, the ear length and the yield of a TaPAB1 over-expressed plant are obviously increased compared with those of a wild type. The invention provides a research basis for increasing the wheat yield and breeding high-yield varieties.

The application discloses protein TaANT1-4D and application of biological materials related to the protein in regulation of ear type traits of plants, and aims to solve the technical problem of how to regulate the ear type traits of plants.The amino acid sequence of the protein TaANT1-4D provided by the application is shown in sequence 2 in the sequence listing.Experiments prove that knocking out TaANT1-4D in a wheat variety Fielder can significantly shorten the ear length, reduce the number of spikelets, the grain length and the thousand-grain weight.Therefore, the protein TaANT1-4D has important application value in regulation of the ear type traits of wheat and has a broad prospect in cultivation of wheat varieties.

The invention belongs to the field of molecular biology, and relates to application of soybean protein GmSEED1 and a coding gene thereof in regulating and controlling the grain weight of seeds. The GmSEED1 is a protein of which the amino acid sequence is as shown in SEQ ID No. 2. Experiments prove that the GmSEED1 and related biological materials thereof can be used for increasing the crop yield and cultivating high-yield varieties.

The invention discloses an application of a gene GmSW6 in regulation and control of plant grain weight and quality. GWAS is developed by using genotype and hundred-grain weight phenotype data, haplotype analysis, gene expression profile data and homologous gene function annotation are combined, and the grain weight related gene GmSW6 is successfully cloned by virtue of research means of molecular biology and comparative genomics. The function of the gene GmSW6 in regulating and controlling the plant grain weight is disclosed for the first time by utilizing a CRISPR-Cas9 system, and the target of increasing the hundred-grain weight of the plant is possibly achieved by knocking out a coding sequence of the GmSW6 gene or silencing the coding gene or reducing the expression level of the coding gene. Meanwhile, by introducing the gene into a target plant or over-expressing the gene in the plant, the grain weight of the plant can be regulated and controlled, the grain weight of the plant can be reduced, but the per unit yield can be increased under the condition, an important gene resource is provided for plant molecular breeding, and a new choice is provided for high yield of the plant.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention belongs to the technical field of plant molecular biology, and discloses application of a soybean GmBZL gene in reduction of soybean grain weight, plant height, oil content and / or improvement of protein content. The soybean GmBZL gene comprises a soybean GmBZL3 gene of which the CDS sequence is as shown in SEQ ID NO: 1 and / or a soybean GmBZL4 gene of which the CDS sequence is as shown in SEQ ID NO: 2. The GmBZL gene is a key gene for regulating and controlling the grain weight, the oil content, the protein content and the plant height of soybeans, valuable gene resources are provided for crop breeding, the soybean GmBZL gene can be widely applied to soybean breeding, and short-plant soybeans with small grains, high protein content and low oil content are obtained.

The application discloses a protein ipa1 with an amino acid sequence as shown in SEQ ID NO:1, which can be expressed in corn to significantly reduce the corn plant height and leaf angle, but does not affect the development of ears and kernels, the hundred-grain weight and ear-grain weight and other agronomic characters, and has important significance for creating short-stalk and compact plant type corn germplasm resources and new variety breeding.

The invention belongs to the field of molecular biology and gene engineering, and particularly relates to a construction method of a transgenic plant, a biological material and application of the biological material. The invention provides a construction method of a transgenic plant, which comprises the step of regulating and controlling the expression level of an OsRLK7 gene in a receptor plant to obtain an OsRLK7 gene overexpressed receptor plant. By improving the expression level of the OsRLK7 gene in a receptor plant, the grain size and thousand grain weight of the receptor plant can be effectively changed, so that the yield of the receptor plant is improved. The OsRLK7 gene is used as a positive regulation factor, and the change of the expression level of the OsRLK7 gene directly affects the development process of receptor plant seeds so as to further affect the size and thousand seed weight of the seeds. The regulation and control mechanism provides a new thought and means for gene function research and breeding work related to the yield of plants such as rice and the like, and has important theoretical value and application prospect.

The invention belongs to the technical field of gene engineering, and particularly relates to application of TaNAS1 protein or a coding gene thereof in regulation and control of plant nicotinamide content. The invention provides application of a TaNAS1 protein or a coding gene thereof in regulating and controlling the content of nicotinamide in plants. The amino acid sequence of the TaNAS1 protein is as shown in SEQ ID NO: 3. The TaNAS1 protein or the coding gene thereof is overexpressed in a plant, so that the nicotinamide content of the plant can be increased, the agronomic traits of wheat can be improved, and the nutrient element content of wheat grains can be increased. Specifically, overexpression of the TaNAS1 protein or the coding gene of the TaNAS1 protein increases the grain length, grain width and hundred-grain weight of wheat, and increases the contents of zinc, iron and copper elements in wheat grains.

The invention discloses an application of OsFRK4 in regulating the interaction of a rice source library to improve the photosynthetic efficiency and the grain weight. The OsFRK4 disclosed by the invention is a protein of which the amino acid sequence is SEQ ID No.3. According to the application disclosed by the invention, the weight of plant grains can be improved by knocking out the OsFRK4 gene, and the source-library synergistic improvement is realized while the crop yield is greatly improved from the aspects of improving the photosynthesis of rice source leaves and accelerating the conversion of carbohydrates in the grains to starch. Therefore, the invention has important application potential for improving crop yield potential and photosynthetic utilization efficiency and realizing source-library synergistic yield increase.