14 results about "Grain number" patented technology

The application discloses a sorghum double-grain trait related gene DG1 and application thereof. The application provides application of any one of the following A1) to A3) in regulation of plant ear grain number, regulation of plant yield and / or cultivation of multiple-grain plants: A1), a protein DG1; A2), a nucleic acid molecule encoding the protein DG1; and A3), a recombinant carrier, an expression cassette or a recombinant bacterium containing the nucleic acid molecule encoding the protein DG1. The application finds that the protein DG1 is a gene for regulating plant ear grain number, overexpression of the gene can increase the plant ear grain number, make the sorghum multiple-grain and realize high yield.

The TaSL1 protein or biological material provided by this invention has the function of increasing the number of grains per ear and the length of the ear in wheat. After reducing the expression of TaSL1 protein in wheat, the transgenic wheat obtained has a significantly increased number of grains per ear, ear length, and awn length compared with the control. The TaSL1 protein or biological material provides a new genetic resource for breeding wheat varieties with high grain count and long ears, which is of great significance for improving wheat yield.

This invention discloses a rice grain-per-panicle number gene, OsTT2, and its applications, belonging to the fields of plant genetic engineering and molecular breeding technology. Through CRISPR / Cas9 gene editing experiments, this invention demonstrates that knocking out the OsTT2 gene significantly reduces the grain-per-panicle number in rice. It also elucidates the molecular mechanism by which OsTT2 influences grain-per-panicle number by directly binding to and activating the co-regulatory factor OsMED15a promoter, thereby regulating downstream grain shape development genes. Furthermore, this invention identifies for the first time a 331 bp fragment duplication variant (SD2) in the OsTT2 gene promoter region associated with the high grain-per-panicle number trait, and based on this, develops molecular markers for selection assistance and corresponding rice breeding methods. This invention provides new key gene resources and precision breeding tools for high-yield rice breeding.

The present application belongs to the field of wheat growth regulation, and particularly relates to a wheat growth regulation method. In view of the technical problem of negative correlation between the growth cycle and yield of wheat, a new method for cultivating wheat is provided. The present application sprays putrescine on the wheat ear, which can effectively shorten the growth cycle of wheat while ensuring the stability of effective tiller number, thousand-grain weight and ear grain number, and avoiding yield loss. The mechanism of action of putrescine may match the endogenous putrescine metabolism characteristics of wheat, realize the synergy of growth regulation and yield maintenance, and effectively improve the protein content.

The application discloses a molecular marker and specific primer related to wheat storage protein and a plurality of spike grain number characters and application. The application belongs to the technical field of biotechnology and particularly relates to a molecular marker and specific primer related to wheat storage protein and a plurality of spike grain number characters and application. The application can be applied to identifying or assisting in identifying wheat storage protein content, spike grain number and wheat breeding by detecting the polymorphism or genotype of an InDel molecular marker in a wheat genome or detecting a substance for genotyping. The InDel molecular marker is a DNA molecule with a nucleotide sequence of 197-437 in SEQ ID No. 3 in the sequence listing. The genotype of the InDel molecular marker is identified by using a primer composition composed of SEQ ID No. 1 and SEQ ID No. 2, so that the efficiency of wheat breeding can be improved, and a dominant variety with high wheat storage protein content and spike grain number can be selected.

This invention discloses AP2 Genes or their encoded proteins and their uses in increasing plant yield or promoting plant growth. This invention relates to genes identified in maize. ZmAP2 The gene was overexpressed in rice, and compared with the control (CK), the overexpressing plants showed a significant increase in panicle grain number, grain length, thousand-grain weight, biomass, and plant height; this invention further uses its homologous rice OsAP2 Gene overexpression in rice ， Compared to the control (CK), the overexpressed rice plants showed a significant increase in the number of grains per panicle, significantly longer grains, significantly increased thousand-grain weight, and significantly higher yield per plant. This invention has potential applications in promoting crop growth, increasing crop yield, and breeding high-yield crop varieties.

The application provides a soybean grain weight major QTL qSW20-1, a developed Indel molecular marker and application thereof, and belongs to the technical field of soybean molecular breeding. The QTL qSW20-1 provided by the application is located on the 20th chromosome of a soybean Glyma.Wm82.a4.v1 genome version, and the interval is reduced to between two SSR markers BARCSOYSSR_20_0830 and BARCSOYSSR_20_0842 by using a residual heterozygote. The same allele of the QTL qSW20-1 and large grain has a significant positive influence on the number of grains per plant and the grain weight per plant, but has no adverse influence on the protein content and the fat content of quality traits. Meanwhile, a molecular marker Indel67 related to the hundred-grain weight is developed, and can be used for molecular marker assisted selection breeding and improving the individual selection efficiency in a breeding population, and accelerating the breeding process.

This invention discloses a gene controlling spike length and grain number per spike in wheat. This gene is located on chromosome 2B using a recombinant inbred line population and high-density SNP markers, within the 657.82–664.40 Mb region of chromosome 2B in the wheat Chinese spring reference genome. A candidate gene, named TaeEF1A, was identified based on the wheat reference genome sequence, RNA-seq, and gene function. Gene editing of TaeEF1A further validated its function in regulating spike length, spikelet number, and grain number per spike. Through gene sequencing and analysis of the gene sequence in a large number of germplasm resources, key variations affecting spike length and grain number per spike were identified in the 3'-UTR of TaeEF1A. Based on this, a detection marker was designed for this gene, which can be used to detect superior allelic variants of the TaeEF1A gene. This marker can be easily, rapidly, and with high throughput applied to marker-assisted breeding to increase spike length, spikelet number, and grain number per spike, thereby increasing yield.

This invention discloses the sorghum double-grain trait gene SbDG2 and its application in regulating double-grain sorghum formation. This invention provides the application of any one of the following substances (A1)-A3) in any one of the following (E1)-E4): E1) regulating the number of grains per spike; E2) regulating plant yield; E3) regulating the development of spikelet florets; E4) regulating the formation of double seeds per spikelet; A1) protein SBDG2; A2) nucleic acid molecule encoding protein SBDG2; A3) recombinant vector, expression cassette, or recombinant bacteria containing a nucleic acid molecule encoding protein SBDG2. This invention clones a gene SbDG2 that controls the double-grain trait in sorghum, and genetic breeding using this gene can effectively increase the number of grains per spike and the yield of sorghum.

This invention discloses the ZmGRAL1 protein, its encoding gene, and its applications, which are related to the stage-specific regulation of maize leaf color phenotype. It belongs to the fields of plant genetic engineering and breeding technology. This invention screened and obtained a maize leaf color mutant. gral1 ,Should gral1 The mutant exhibits a significant albino phenotype before V5, followed by regreening; agronomic traits such as plant height, 100-grain weight, and grain number per ear are not different from the wild type, making it a leaf color mutant with application value. For analysis... gral1 The molecular mechanism of mutant phenotype formation was isolated in this invention using map-based cloning technology. ZmGRAL1 Genes, and verified through gene knockout. ZmGRAL1 Genes that regulate the development of maize leaf color. ZmGRAL1 Genes can regulate chloroplast development and stage-specific regulation of maize leaf phenotype, and can be used as maternal marker genes to efficiently identify false hybrids through leaf color during the seedling stage.

This invention belongs to the field of wheat germplasm resource identification technology, and more specifically, relates to the KASP molecular marker for wheat grain number, primers, and their applications. The marker provided by this invention is located on wheat chromosome 5A, and its nucleotide sequence is shown in SEQ ID NO.1. The SNP site polymorphism is A or C, with the CC genotype significantly correlated with high grain number per spike. Specific primers designed based on this marker can be used for efficient identification of wheat grain number traits, assisting in the selection of high-yielding varieties in breeding. Through genome-wide association analysis and haplotype verification, this marker is stably expressed in multiple environments, with a phenotypic explanation rate of approximately 14%. This invention provides a practical tool for marker-assisted breeding of wheat yield traits and has significant application value.

This invention discloses a method for creating new germplasm of large-spike, large-grain wheat. The method involves hybridizing dwarf wheat, large-spike wheat varieties or lines, and large-grain wheat varieties or lines as parents to obtain dwarf hybrid seeds. Half of the dwarf hybrid seeds are planted, and superior fertile plants with large spikes or large grains are selected. The other half of the dwarf hybrid seeds are then planted and hybridized with the selected superior fertile plants with large spikes or large grains to obtain a dwarf wheat selection population. Superior fertile plants with large spikes and large grains are obtained from this selection population, and through continuous self-pollination, these superior plants are selected to obtain new large-spike, large-grain wheat lines. This invention clarifies the construction method and selection criteria for large-spike, large-grain wheat selection populations, significantly improving the breeding efficiency of large-spike, large-grain wheat. The newly bred large-spike, large-grain wheat lines increase the number of grains per spike and the thousand-grain weight, enhancing the yield potential of wheat and providing important germplasm support for high-yield wheat breeding and yield improvement.

The application discloses application of protein TaeIF4A in increasing grain number and yield of a wheat main ear. Experiments prove that overexpression of the protein TaeIF4A in an A genome can significantly increase plant height, tiller number, ear length, grain number of a main ear and yield per plant of the wheat; the coding gene of the protein TaeIF4A in the A genome is edited by a gene, and the coding gene of the protein TaeIF4A in a D genome is edited by a gene TaeIF4A-6A , and when the coding genes of the protein TaeIF4A in the A genome and / or the D genome are all mutated to cause early termination of protein translation, the plant height, tiller number, ear length, grain number of a main ear and yield per plant of the wheat can be significantly reduced. TaeIF4A-6D TaeIF4A-6A TaeIF4A-6D Therefore, the protein TaeIF4A can regulate yield, grain number of a main ear, plant height, tiller number and / or ear length of the wheat. The application has important application value.​​