95 results about "Grain number" patented technology

The invention discloses an SNP (Single Nucleotide Polymorphism) site related to the number of spikelet per ear and the number of grains per ear of wheat and application of the SNP site. The SNP site comprises a kit for identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat, primers and application of the elements in identifying or assisting in identifying the number of spikelet per ear and the number of grains per ear of wheat. The invention provides a new method for molecular marker-assisted selective breeding of wheat, and has important significance in cultivation and research of high-yield wheat varieties.

The invention provides application of an OsRTD1 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsRTD1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the OsRTD1 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of the OsRTD1 gene mutant plant osrtd1 under the drought stress of PEG induction and soil dehydration is remarkably improved, the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced, the relative water loss rate of dehydrated leaves is higher, and the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced. The total grain number, the solid grain number, the total grain weight and the like of the OsRTD1 gene mutant plant osrtd1 under the stress of field drought are obviously higher than those of a wild plant. The OsRTD1 gene is a potential candidate new target for regulating and controlling a rice drought resistance mechanism.

The invention discloses an intelligent detection method and device for a cotton water miscellaneous micronaire value based on multi-source information fusion, and relates to the field of cotton quality detection.The method comprises the steps that the weight, the initial air pressure difference, the cotton sample image, the resistance value, the environment temperature and the environment humidity of a to-be-detected cotton sample are obtained; according to the initial air pressure difference and the weight, the compensated air pressure difference is determined through a weight compensation model; according to the cotton sample image, utilizing an image segmentation technology to extract the impurity area percentage and the impurity grain number of the cotton sample to be detected, and determining the impurity content of the cotton sample to be detected; according to the resistance value, the environment temperature and the environment humidity, utilizing a moisture regain prediction model to determine the moisture regain of the cotton sample to be detected; and determining the micronaire value of the cotton sample to be tested by using a Stacking ensemble learning model according to the compensated air pressure difference, impurity content and moisture regain. According to the method, the moisture regain, the impurity rate and the micronaire value of the cotton can be accurately detected in a non-standard detection environment.

The invention discloses a corn ear grain number, ear row number and ear row grain number detection method, and belongs to the field of image detection.According to the corn ear grain number counting method, multi-scale features of a corn ear image are encoded through a Transform encoder, the long-distance dependency relationship and global context information between the features are enhanced, and the detection accuracy is improved. A decoder outputs feature representation of each query point and provides rich semantic information for a point query and Transform decoding module, the decoder outputs feature representation of each query point, rich semantic information is provided for classification and positioning tasks of prediction heads, background interference can be effectively eliminated, and counting precision is improved; according to the method for detecting the ear row number and the ear row grain number, common problems in half-row corn ear row detection, nonlinear corn ear row detection and the like are solved by utilizing clustering and a vector-based point searching updating iteration method, and row detection and ear row number detection can be efficiently and accurately realized.

The invention discloses application of a wheat nitrogen response gene TaNIA1 in cultivation of high-yield wheat. The invention belongs to the technical field of biology, and particularly relates to application of a wheat nitrogen response gene TaNIA1 in cultivation of high-yield wheat. The protein TaNIA1 disclosed by the invention can be applied to the following aspects: 1) application to regulation and control of plant yield; 2) application in preparation of products for regulating and controlling plant yield; the invention relates to application of TaNIA1 in cultivation of plants with changed yield, application of TaNIA1 in preparation of products for cultivation of plants with changed yield, and application of TaNIA1 in plant breeding. TaNIA1 influences nitrogen absorption and utilization of wheat, so that agronomic traits such as wheat tiller number, grain number per ear, ear length, hundred-grain weight and the like are remarkably increased, and the yield of wheat is finally influenced.

The invention discloses a wheat grain number related C783T SNP (single nucleotide polymorphism) and application thereof, the SNP site corresponds to the 783th basic group from the 5'tail end of the sequence shown as SEQ ID NO: 1, namely the C783T SNP site; the sites are C / T polymorphisms. The invention also develops a reagent or a kit for identifying or assisting in identifying the character of the number of wheat ears based on the SNP site, and provides a method for identifying or assisting in identifying the genotype of wheat. Through experimental verification of a large number of wheat materials, it is found that the grain number of wheat with the homozygous genotype C / C is larger than or candidate larger than the grain number of wheat with the homozygous genotype T / T. The SNP has high effectiveness and potential application value, wheat with high grain number per ear can be found by detecting the SNP, and the SNP has important value in research or application of cultivation of high-yield wheat varieties.

The invention discloses application of a TaSPL18-5D protein and a related biological material thereof to regulation and control of the ear length and the yield of a plant. According to the invention, a TaSPL18-5D overexpression wheat strain is obtained through a transgenic technology, and then analysis on the TaSPL18-5D overexpression wheat strain finds that compared with wild type wheat KN199, the TaSPL18-5D overexpression wheat strain has the advantages that the ear length is shortened, and the spikelet number and the ear grain number are obviously reduced. Therefore, the TaSPL18-5D protein can negatively regulate the ear length, the spikelet number and the grain number per ear of the plant, a new target is provided for improving the plant type and the yield of the wheat after green revolution, and the TaSPL18-5D protein has important significance for cultivating high-yield wheat varieties in agricultural production.

The invention discloses an application of NOG2 protein in regulation and control of plant yield. The invention provides application of protein NOG2, a substance for regulating and controlling expression of coding nucleic acid of the protein or a substance for regulating and controlling activity or content of the protein in any one of the following aspects: regulating and controlling plant yield, grain number per spike and / or grain length; plants with high yield, increased grain number per ear and / or increased grain length are prepared; according to the present invention, the expression of the coding nucleic acid of the protein NOG2 in the plant is down-regulated, weakened or reduced so as to improve the plant yield, the grain number per ear and / or the grain length, and the coding nucleic acid of the protein NOG2 is the high-quality gene for culturing the high-quality rice variety, and is used for culturing the high-yield rice.

The application discloses a method for screening wheat with different thousand-grain weights and ear phenotypes, and comprises the following steps: detecting whether the genotype of a wheat to be tested based on a TaNAC92-2A gene is genotype I or genotype II, the ear length, the number of grains per ear, the number of fertile spikelets and / or the number of spikelets per ear of the wheat with genotype I are greater than those of the wheat with genotype II; the thousand-grain weight of the wheat with genotype I is less than that of the wheat with genotype II; the genotype of the wheat with genotype I based on an A415G SNP site is AA homozygous type; the genotype of the wheat with genotype II based on the A415G SNP site is GG homozygous type; and the A415G SNP site is the 415th nucleotide from the 5' end in the wheat genome, as shown in SEQ ID NO: 1. By detecting the genotype of the wheat to be tested based on the TaNAC92-2A gene, the wheat thousand-grain weight and / or the ear phenotype can be screened. The application has important application value.

The application discloses a sorghum double-grain trait related gene DG1 and application thereof. The application provides application of any one of the following A1) to A3) in regulation of plant ear grain number, regulation of plant yield and / or cultivation of multiple-grain plants: A1), a protein DG1; A2), a nucleic acid molecule encoding the protein DG1; and A3), a recombinant carrier, an expression cassette or a recombinant bacterium containing the nucleic acid molecule encoding the protein DG1. The application finds that the protein DG1 is a gene for regulating plant ear grain number, overexpression of the gene can increase the plant ear grain number, make the sorghum multiple-grain and realize high yield.

The invention discloses an AP2 gene or an encoding protein thereof and application of the AP2 gene or the encoding protein thereof in increasing plant yield or promoting plant growth. The ZmAP2 gene is identified from corn and is overexpressed in rice, and compared with a control CK, the number of grains per ear of an overexpressed plant is remarkably increased, the grain length is remarkably increased, the thousand grain weight is remarkably increased, the biomass is remarkably increased, and the plant height is remarkably increased; the homologous rice OsAP2 gene is further subjected to overexpression in rice, and compared with a control CK, the overexpressed rice plant grain number per ear is remarkably increased, grains are remarkably lengthened, the thousand grain weight is remarkably increased, and the yield of a single plant is remarkably increased. The invention has application potential in the aspects of promoting crop growth, improving crop yield, cultivating high-yield crop varieties and the like.

The TaSL1 protein or biological material provided by this invention has the function of increasing the number of grains per ear and the length of the ear in wheat. After reducing the expression of TaSL1 protein in wheat, the transgenic wheat obtained has a significantly increased number of grains per ear, ear length, and awn length compared with the control. The TaSL1 protein or biological material provides a new genetic resource for breeding wheat varieties with high grain count and long ears, which is of great significance for improving wheat yield.

The invention belongs to the technical field of plant bioengineering, and discloses a KASP primer group for detecting wheat plant height and yield synergistically improved QTL locus QPH-6A and application, the primer group is designed for SNP loci at the physical positions 11, 164 and 425 bp of wheat 6A chromosomes, and the primer group comprises two specific forward primers for different alleles and a universal reverse primer. By utilizing the primer group and the detection method provided by the invention, whether the wheat material carries the QPH-6A excellent allele or not can be quickly and accurately identified. The excellent allelic gene has a unique'dwarfing without yield reduction 'genetic effect, and the thousand seed weight, the ear length and the ear seed number can be remarkably increased while the plant height is reduced to a suitable lodging-resistant range (75-85 cm). According to the invention, the problems of too short plant, reduced biomass, premature senility and the like caused by the traditional dwarf gene in arid ecological regions such as Xinjiang are solved, and an efficient breeding tool is provided for cultivating a new high-yield ideal plant type wheat variety suitable for an arid environment.

The invention discloses a method for regulating and controlling the spike number per plant, the grain number per spike or / and the yield of a plant under a low-nitrogen condition, and belongs to the technical field of biology. The method comprises the step of regulating and controlling the ear number of a single plant, the grain number per ear or / and the yield of the plant by regulating and controlling coding gene expression of protein in the plant or regulating and controlling the activity and / or the content of the protein, the protein is a protein with an amino acid sequence of SEQ ID NO: 1. Experiments prove that the yield of grains can be remarkably increased by knocking down TaNIP1.

The invention discloses application of a rice gene OsGN6 to regulation and control of rice grain number per spike and yield. The invention provides application of OsGN6 protein or related biological materials thereof in regulation and control of grain number per ear and yield of plants. According to the invention, an OsGN6-BYP gene is mutated by applying a CRISPR-Cas9 method, and it is found that the OsGN6-BYP gene can significantly reduce the number of grains per ear of rice; and overexpression of the OsGN6-BYP gene can significantly increase the number of grains per ear of rice. Therefore, the OsGN6 gene plays an important role in regulating and controlling the grain number per ear of rice and increasing the yield, and has important significance for cultivating new varieties of high-yield plants.

The invention provides a molecular marker linked with a major QTL (Quantitative Trait Loci) of the total grain number of a single rice plant and application of the molecular marker. The molecular marker is TGN5. A new major QTL TGN5 for controlling the total grain number of a single plant of the super rice Y900 is identified, is positioned in a section of about 1.46 Mb of a long arm of a chromosome 5, and just falls in a hot spot region Chr5: 17.9-27.3 Mb range of indica-japonica genetic differentiation of parents; the TGN5 is reduced to an interval of about 251.39 Kb by constructing a local genetic linkage map, and 13.3% of phenotypic variation is explained to the maximum extent; the loci are verified in segregation populations of the super rice Y1 and Y2 at the same time, and the single plant total grain number heterosis of the three-stage super rice can be differentially influenced. The molecular marker provided by the invention can be applied to regulation and control of the total grain number of a single rice plant.

This invention relates to the field of scientific fertilization and production technology for rice, specifically to a method and application of panicle-stage fertilization to stabilize the number of grains per panicle and improve the panicle type index. The fertilization period is the third leaf from the bottom, which is the stage from the third leaf from the bottom until it is fully expanded. The fertilization amount is 110 kg / ha. ‑1 The fertilizer is urea, and the total nitrogen content of the urea is ≥46%. The nitrogen management method during the heading stage of this invention stabilizes the number of grains per panicle while improving the panicle shape index, thus resolving the synergistic contradiction between the number of grains per panicle and the grain fillability. Compared with the conventional method of applying nitrogen fertilizer during the heading stage at the three-leaf stage, this invention can cultivate a grain-setting characteristic dominated by grains in the middle and upper parts of the panicle, increasing the rice panicle shape index by 8.8%–10.0% and achieving a yield increase of 8.8%–9.1%.

The invention provides an oat grain number counting method, system and device based on deep learning, and the method comprises the steps: collecting an oat image data set, and carrying out the marking and data enhancement of the data set; constructing an oat grain counting model based on deep learning; the model comprises a backbone network, a feature pyramid module, a rotating frame detection head and a counting module which are connected in sequence. According to the oat image data set after marking and data enhancement, training the oat grain number counting model based on deep learning to obtain a trained oat grain number counting model based on deep learning; and obtaining a to-be-counted oat image, and obtaining the oat grain number based on the trained deep learning-based oat grain number counting model. According to the oat grain number counting method based on deep learning provided by the invention, multi-scale feature extraction and rotating frame detection technologies are adopted, model training is carried out in combination with mass focusing loss and rotation IoU loss, and the accuracy and efficiency of oat grain number counting are effectively improved.

The invention belongs to the technical field of agriculture, and particularly relates to a wheat filling stage shading stress test sampling method which comprises the following steps: selecting wheat of the same variety, and selecting a group of main stem ears which are consistent in growth vigor and bloom on the same day on the bloom day of the wheat of the variety for marking; respectively carrying out post-flowering shading treatment and non-shading treatment on the main stem ears with the marks; from the grain filling stage to the mature stage of the wheat, sampling the processed main stem ears at intervals, and recording agronomic character indexes of the main stem ears; the average ear grain number of the main stem ears subjected to shading treatment is used as a reference number, the main stem ears with the ear grain number close to the reference number in non-shading treatment are selected as non-shading samples, and the difference value range of the average ear grain number of the selected non-shading samples and the reference number is-1.5-1.5; according to the method, the sampling error is reduced, the shading test sampling process in the wheat filling period is optimized, and a new data analysis method is provided for the wheat filling test.

The invention discloses a molecular marker related to corn row grain number and application thereof, the molecular marker is based on a corn ZmSBP20 gene promoter region, the ZmSBP20 is located on a corn chromosome 4, the physical position of the ZmSBP20 on a corn B73 reference genome Zm-B73-REFERENCE-NAM-5. 0 is Chr4: 246387302-246390553, the corresponding physical position of the molecular marker is Chr4: 246391202-246391344, and the ZmSBP20 is located on the corn ZmSBP20 gene promoter region. The base sequence of the InDel + genotype at the position is as shown in SEQ ID No.1, and the InDel-genotype at the position is deleted by 142 bp. The molecular marker has the characteristics of stable amplification, high detection efficiency and the like, can accurately, efficiently and stably distinguish the InDel + genotype from the InDel-genotype, can be widely applied to molecular marker-assisted selection of favorable variation sites of row grain number, and provides important technical support and theoretical basis for genetic improvement of corn yield.

This invention discloses a rice grain-per-panicle number gene, OsTT2, and its applications, belonging to the fields of plant genetic engineering and molecular breeding technology. Through CRISPR / Cas9 gene editing experiments, this invention demonstrates that knocking out the OsTT2 gene significantly reduces the grain-per-panicle number in rice. It also elucidates the molecular mechanism by which OsTT2 influences grain-per-panicle number by directly binding to and activating the co-regulatory factor OsMED15a promoter, thereby regulating downstream grain shape development genes. Furthermore, this invention identifies for the first time a 331 bp fragment duplication variant (SD2) in the OsTT2 gene promoter region associated with the high grain-per-panicle number trait, and based on this, develops molecular markers for selection assistance and corresponding rice breeding methods. This invention provides new key gene resources and precision breeding tools for high-yield rice breeding.

The application discloses application of a rice OsPTH1 gene in improving plant nitrogen utilization efficiency and yield increase, and relates to the technical field of genetic engineering.The OsPTH1 mutant plant is obtained through CRISPR-Cas9 technology.Further experiments prove that, compared with wild-type rice, the mutant plant pth1 strain has increased effective tillering, shortened heading stage, increased grain number per panicle, changed dwarf plant height, increased yield per plant and improved nitrogen utilization efficiency.The application proves that the OsPTH1 gene can regulate rice yield and nitrogen utilization efficiency, and lays a foundation for cultivating new rice varieties with high yield and high nitrogen utilization efficiency.

The present invention discloses a KASP molecular marker for regulating wheat grain number and plant height genes and its application, which relates to the field of biotechnology; the primer set of the KASP molecular marker for identifying wheat grain number and plant height genes comprises three primers whose nucleotide sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3 respectively. The present invention also applies the KASP molecular marker to wheat <h2 style=";text-align:left;direction:ltr">TaTPP-7D Detection, identification, and auxiliary identification of gene SNP allele variation in wheat grain number and plant height traits or molecular marker-assisted breeding. The present invention develops relevant primer sets based on wheat grain number and plant height traits for detecting SNP allele variation sites of related traits. This has the advantages of being quick and convenient, with fast identification speed and high accuracy, improving wheat breeding efficiency, promoting the development of the wheat industry, and has broad application prospects.

The invention relates to a composition for increasing wheat grain weight, a growth regulator, a preparation method and application, and the growth regulator contains the composition with the active ingredients of chlorfenuron, 2, 4-epibrassinolide and an amino acid water-soluble fertilizer. According to the growth regulator for increasing the grain weight of the wheat, provided by the invention, the chlorfenuron, the 2, 4-epibrassinolide and the amino acid water-soluble fertilizer can be specifically combined and proportioned, so that the drought resistance and the photosynthetic efficiency of the wheat in the grain filling period are improved based on a synergistic effect, and grain filling is promoted; in addition, the thousand seed weight and the grain number per spike are remarkably increased, and the grain yield is stabilized; meanwhile, the preparation method has the advantages of safety, non-toxicity, high efficiency and environmental protection, is simple and easy to operate and is convenient for agricultural popularization and application.

The present application belongs to the field of wheat growth regulation, and particularly relates to a wheat growth regulation method. In view of the technical problem of negative correlation between the growth cycle and yield of wheat, a new method for cultivating wheat is provided. The present application sprays putrescine on the wheat ear, which can effectively shorten the growth cycle of wheat while ensuring the stability of effective tiller number, thousand-grain weight and ear grain number, and avoiding yield loss. The mechanism of action of putrescine may match the endogenous putrescine metabolism characteristics of wheat, realize the synergy of growth regulation and yield maintenance, and effectively improve the protein content.

The invention belongs to the technical field of biology, and particularly relates to application of a rice OsC3H33 gene to regulation and control of plant grain sizes. According to the invention, under the background of different rice varieties, the mutants of the osc3h33-1 and the osc3h33-2, which are lack of functions, are successfully obtained. Researches find that the two mutants both have the phenomena that the number of grains per ear is reduced, and the grain length and thousand grain weight are reduced. Cytological observation shows that the longitudinal cell width and length of the osc3h33-1 and osc3h33-2 mutants are obviously reduced, the length of the transverse cell of the osc3h33-1 is not obviously changed, but the width is reduced. When the OsC3H33 mutant is applied to rice, the grains of the two mutants become smaller, and the cell area becomes smaller, which indicates that the OsC3H33 can adjust the grain size by influencing the cell expansion under different rice backgrounds, and further influence the thousand grain weight. In addition, the chalkiness degree of the grains of the mutant after the OsC3H33 gene is knocked out is obviously increased compared with that of the wild type.

The present application relates to a method and device for simulating the crystal plasticity of micro-fatigue of a twin-crystal alloy containing small-angle grain boundaries. The method comprises: performing EBSD characterization on a twin-crystal tenon specimen containing small-angle grain boundaries, generating an inp file from the EBSD results using Dream3d, setting parameters such as grain number and crystal orientation for the inp file using Matlab, importing the input file into ABAQUS to obtain an ABAQUS finite element model, and setting the load, boundary conditions, analysis step, friction and contact conditions. The file is then associated with a Fortran program written based on the crystal plasticity constitutive relation, and the stress, strain and cumulative plastic strain results are simulated. Thus, by establishing a finite element model consistent with the crystal microstructure of the actual specimen, applying the crystal plasticity constitutive relation to each grain, and assigning the true crystal orientation of each grain to each grain, the crystal plasticity simulation of the micro-fatigue specimen is achieved.

The application discloses a molecular marker of a wheat TaACL5-4A gene and application thereof, and belongs to the technical field of crop selection and cultivation. The molecular marker is a KASP marker, which is obtained by amplification of a primer group shown in SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, the 5' ends of the two front primers are marked with HEX and FAM respectively, the PCR amplification product is detected by using an enzyme marker, and KlusterCaller software is used for genotyping. The application has the advantages that by applying the KASP molecular marker developed by the application, the ear grain number, ear length and spikelet number and the like of the wheat can be predicted, the cost is saved, the selection efficiency is greatly improved, the breeding process can be accelerated, and new possibilities are provided for efficient screening of excellent alleles of the TaACL5-4A gene and cultivation of high-yield wheat varieties.

The present invention discloses a SNP‑C682T related to wheat grain number per spike and its application. The SNP site corresponds to the 682nd base from the 5' end of the sequence shown in SEQ ID NO.1. When the site is C / C homozygous, the corresponding genotype is A; when the site is T / T homozygous, the corresponding genotype is B. The grain number per spike is: wheat homozygous for genotype A is greater than, or is potentially greater than, wheat homozygous for genotype B. The SNP of the present invention has high effectiveness and potential application value. By detecting the SNP, wheat with a larger grain number per spike can be found, which is of great value in the research or application of breeding high-yield wheat varieties.

The application discloses a molecular marker and specific primer related to wheat storage protein and a plurality of spike grain number characters and application. The application belongs to the technical field of biotechnology and particularly relates to a molecular marker and specific primer related to wheat storage protein and a plurality of spike grain number characters and application. The application can be applied to identifying or assisting in identifying wheat storage protein content, spike grain number and wheat breeding by detecting the polymorphism or genotype of an InDel molecular marker in a wheat genome or detecting a substance for genotyping. The InDel molecular marker is a DNA molecule with a nucleotide sequence of 197-437 in SEQ ID No. 3 in the sequence listing. The genotype of the InDel molecular marker is identified by using a primer composition composed of SEQ ID No. 1 and SEQ ID No. 2, so that the efficiency of wheat breeding can be improved, and a dominant variety with high wheat storage protein content and spike grain number can be selected.