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Gene for controlling Oryza sativa plant height, enhancing lodging resistance, increasing effective tiller number and yield and its application

A technology of anti-lodging and tiller number, applied in the field of plant biology

Active Publication Date: 2014-08-20
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the sd1 gene has been widely used in current agricultural production, the problem of lodging is still a bottleneck for high-yield breeding

Method used

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  • Gene for controlling Oryza sativa plant height, enhancing lodging resistance, increasing effective tiller number and yield and its application
  • Gene for controlling Oryza sativa plant height, enhancing lodging resistance, increasing effective tiller number and yield and its application
  • Gene for controlling Oryza sativa plant height, enhancing lodging resistance, increasing effective tiller number and yield and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Embodiment 1: Construction of SDT near isogenic line

[0126] The inventor used the conventional japonica rice variety Wandao 44 (approved in Anhui in 1997, serial number: Wanpinshen 97010204) as the recurrent parent, and carried out continuous multiple generations of backcrossing with the naturally mutated semi-dwarf multi-tiller mutant sdt as the donor parent . in bc s f 2 In the population, individual plants were selected based on the phenotype of decreased plant height, increased tiller number, and PCR tracking of the stuck target segment. In addition to selecting the target segment, background scanning was also performed on other chromosome segments other than the target trait, and finally the near isogenic lines Wandao 44-sdt (WD44-sdt) and Rice 44-SDT (WD44-SDT) material was used for follow-up research. For the phenotypes of near-isogenic lines WD44-sdt and WD44-SDT, see figure 1 . According to the comparative analysis of the phenotypes of near-isogenic li...

Embodiment 2

[0132] Example 2: Comparative Study on Agronomic Characters of SDT Near-Isogenic Lines

[0133] The present inventor sows WD44-SDT and WD44-sdt in the experimental base of Science Island in Hefei City, Anhui Province and the experimental base in Lingshui County, Hainan Province respectively (the construction of near-isogenic lines WD44-SDT and WD44-sdt is detailed in Example 1. Introduction, continuous backcrossing for 7 generations to obtain near-isogenic lines), after the plants mature, count the plant height, number of tillers per plant, lodging index, and number of branches per plant (number of tillers per plant). of primary branches), number of secondary branches, panicle length, thousand grain weight (1,000 grain weight) and number of grains per panicle (number of grains per panicle) and yield per plant. See figure 1 .

[0134] Concrete statistical method: after the paddy rice matures, take the panicles on 120 main tillers in the field respectively, count the number o...

Embodiment 3

[0137] Example 3: Controlling Rice Plant Height, Improving Lodging Resistance, Increasing Tiller Number and Yield Gene Acquisition

[0138] The present invention utilizes rice late 3 (higher plant height, average value 108cm) and semi-dwarf multi-tiller sdt mutant (lower plant height, average value 78cm) to construct BC 1 f 2 Groups and BC 1 f 7 polymorphic markers were developed, and the sdt gene was cloned by using map-based cloning technology. The genomic DNA sequence (WD44-sdt gDNA) of the gene is shown in SEQ ID NO:4. The gene contains 4 introns, and the length of the mRNA sequence produced by transcription is 987nt, as shown in SEQ ID NO: 2. An mRNA with a length of 1552nt is transcribed in Wandao 44, as shown in SEQ ID NO: 1. Comparing the DNA sequence differences between WD44-sdt and WD44-SDT, it can be seen that in WD44-sdt there is a deletion of 131 bp from the middle of the second exon to the second intron (1281-1411) of the SDT gene, and the deletion position R...

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Abstract

The invention discloses an SDT (semi dwarf and high-tillering) gene for decreasing Oryza sativa plant height, enhancing lodging resistance, increasing tiller number and yield, and its application. The SDT gene coding is OsmiR156h. In semi dwarf and high-tillering SDT Oryza sativa mutant, the length of the mRNA 3'-UTR transcribed from the SDT gene is shorter than that of the 3'-UTR transcribed from a wild type SDT gene, resulting in high SDT gene transcription level than expression level of the wild type SDT gene. Genetic analysis shows that SDT is a semidominant mutation, which can inhibit longitudinal splitting of stem cells to control the internode length and plant height, thereby improving the lodging resistance of Oryza sativa; at the same time, the SDT gene can increase effective tiller number of Oryza sativa, thereby improving grain number and yield per spike. The present invention for the first time improves that the control on the length of 3'-UTR of mRNA transcribed from the OsmiR156h gene can change the expression level of gene, thereby realizing the application prospect of the cultivation of excellent varieties of Oryza sativa.

Description

technical field [0001] The invention belongs to the field of plant biotechnology. Specifically, the present invention relates to a gene for controlling rice plant height, improving lodging resistance, increasing effective tiller number and yield and application thereof. The present invention also relates to the sequence of the non-coding region of the gene. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in the world, and the improvement of rice yield is related to ensuring food security and sustainable agricultural development. In the current agricultural production, the improvement of rice yield depends on a large amount of external fertilization, but this will increase the risk of lodging and yield reduction in the mature stage of rice, thereby limiting the amount of fertilizer application. The solution to this contradiction is the widespread use of the rice semi-dwarf gene sd1 1，2 ——This gene is also known as the "green rev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C12N1/21A01H1/02A01H5/00C12N15/113
CPCC12N15/8216C12N15/8261
Inventor 傅向东赵孟吴昆吴跃进刘斌美刘倩
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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