The invention relates to a method for bovine in-vitro fertilized
embryo culture and a used culture solution. Particularly, on one hand, the invention relates to a method for culturing a bovine in-vitro fertilized
embryo. The method comprises the following steps of putting the bovine in-vitro fertilized
embryo into a bovine in-vitro fertilized
embryo culture solution and carrying out embryo in-vitro culture under the conditions that the temperature is 38.5 DEG C, the content of CO2 is 0.5% and the
humidity is 100%. The invention further relates to a bovine in-vitro fertilized
embryo culture solution. The culture solution comprises NaCl, KCl, NaHCO3, MgCl2, KH2PO3,
sodium pyruvate, glucose,
calcium galactonate, fetal calf serum, L-
glutamine, an essential amino-acid, a nonessential
amino acid,
glutathione and water. The method and the used culture solution have excellent technical effects in the specification, for example, the method has a higher
blastocyst hatching rate when the bovine in-vitro fertilized embryo is cultured.