A safe recording and comparison method for artificial fertilization, in-vitro fertilization and embryo transfer comprises the following steps of: step 1, archiving of a male parent and a female parent: storing identity information, pairing information and biological feature information; step 2, semen collection: identifying male parent information at first, then collecting semen, and establishing a matching relation between the semen and the male parent; step 3, semen treatment: during treatment, checking whether the semen is matched with the male parent in each sample transfer or transmitting the matching relation; step 4, ovum collection: identifying female parent information at first, then collecting an ovum, and establishing a matching relation between the ovum and the female parent; step 5, ovum fertilization: identifying semen and ovum information at first, then checking whether pairing is allowed, and if so, continuing, or otherwise, sending out an error prompt; and step 6, transplantation: verifying the female parent information at first, then checking whether an embryo is matched with the female parent, and if so, continuing, or otherwise, sending out the error prompt. The checking is performed before every operation to prevent errors.