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3 results about "External fertilization" patented technology

External fertilization is a mode of reproduction in which a male organism's sperm fertilizes a female organism's egg outside of the female's body. It is contrasted with internal fertilization, in which sperm are introduced via insemination and then combine with an egg inside the body of a female organism. External fertilization typically occurs in water or a moist area to facilitate the movement of sperm to the egg. The release of eggs and sperm into the water is known as spawning. In motile species, spawning females often travel to a suitable location to release their eggs. However, sessile species are less able to move to spawning locations and must release gametes locally. Among vertebrates, external fertilization is most common in amphibians and fish. Invertebrates utilizing external fertilization are mostly benthic, sessile, or both, including animals such as coral, sea anemones, and tube-dwelling polychaetes. Benthic marine plants also use external fertilization to reproduce. Environmental factors and timing are key challenges to the success of external fertilization. While in the water, the male and female must both release gametes at similar times in order to fertilize the egg. Gametes spawned into the water may also be washed away, eaten, or damaged by external factors.

Transgenic rainbow shark

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
Owner:GLOFISH LLC

Laser-assisted in vitro fertilization

The present invention provides a method for improving the efficiency of in vitro fertilization in animals. The method comprises: (a) obtaining oocytes from the animal, wherein the oocytes include the oocyte body and the zona pellucida; (b) obtaining sperm from the animal; (c) maturing the oocytes in a maturation medium; (d) removing cumulus cells from the oocytes; (e) creating holes in the zona pellucida of the oocytes with a laser; (f) contacting the oocytes with the sperm; and (g) incubating the oocytes with the sperm, wherein the incubation enables in vitro fertilization of the oocytes and sperm, and an animal embryo is produced; and the efficiency of in vitro fertilization for producing an animal embryo is improved by creating holes in the zona pellucida of the oocytes with a laser. In certain embodiments, the cumulus cells are removed from the oocytes by treatment with hyaluronidase, pipetting, and / or vortexing. In one embodiment, the oocytes are placed on a microscope stage prior to step (e).
Owner:COLOSSAL BIOSCIENCES INC

Methods, uses, devices, computer equipment and storage media for diagnosing routine in vitro fertilization results of sperm

ActiveCN116482379BObstetricsPhysiology
The application relates to a method, device, computer equipment and storage medium for diagnosing the result of routine in vitro fertilization of sperm. The method for diagnosing and predicting the result of routine in vitro fertilization comprises the following steps: calculating the PLCzeta positive rate of a sperm sample, the PLCzeta positive rate being the proportion of the number of PLCzeta positive sperm in the sperm sample in the total number of sperm, the PLCzeta positive sperm being sperm in which PLCzeta can be detected in at least one of the following head regions of the sperm: acrosome, equator and post-acrosomal region; and diagnosing the fertilization result of routine in vitro fertilization according to the PLCzeta positive rate. The method can efficiently judge the fertilization capacity of sperm that has undergone or is to undergo routine in vitro fertilization, find sperm that may have low fertilization or fertilization failure in routine in vitro fertilization due to PLCzeta defects, provide a basis for the development of a fertilization scheme, and prevent the occurrence of fertilization failure or low fertilization.
Owner:REPRODUCTIVE & GENETIC HOSPITAL OF CITIC XIANGYA CO LTD +1