The present invention provides a method for improving the efficiency of
in vitro fertilization in animals. The method comprises: (a) obtaining oocytes from the animal, wherein the oocytes include the
oocyte body and the
zona pellucida; (b) obtaining
sperm from the animal; (c) maturing the oocytes in a maturation medium; (d) removing cumulus cells from the oocytes; (e) creating holes in the
zona pellucida of the oocytes with a
laser; (f) contacting the oocytes with the
sperm; and (g) incubating the oocytes with the
sperm, wherein the incubation enables
in vitro fertilization of the oocytes and sperm, and an animal
embryo is produced; and the efficiency of
in vitro fertilization for producing an animal
embryo is improved by creating holes in the
zona pellucida of the oocytes with a
laser. In certain embodiments, the cumulus cells are removed from the oocytes by treatment with
hyaluronidase, pipetting, and / or vortexing. In one embodiment, the oocytes are placed on a
microscope stage prior to step (e).