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170 results about "External fertilization" patented technology

External fertilization is a mode of reproduction in which a male organism's sperm fertilizes a female organism's egg outside of the female's body. It is contrasted with internal fertilization, in which sperm are introduced via insemination and then combine with an egg inside the body of a female organism. External fertilization typically occurs in water or a moist area to facilitate the movement of sperm to the egg. The release of eggs and sperm into the water is known as spawning. In motile species, spawning females often travel to a suitable location to release their eggs. However, sessile species are less able to move to spawning locations and must release gametes locally. Among vertebrates, external fertilization is most common in amphibians and fish. Invertebrates utilizing external fertilization are mostly benthic, sessile, or both, including animals such as coral, sea anemones, and tube-dwelling polychaetes. Benthic marine plants also use external fertilization to reproduce. Environmental factors and timing are key challenges to the success of external fertilization. While in the water, the male and female must both release gametes at similar times in order to fertilize the egg. Gametes spawned into the water may also be washed away, eaten, or damaged by external factors.

System for in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comrprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY

In-Vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY

Method for establishing ferret model capable of applying to study human disease and application thereof

The invention relates to a method for establishing a ferret model capable of applying to study human disease and an application thereof, which belong to the biology field and specifically relates to a ferret ovulation induction technology and a ferret external fertilization technology. With the method for establishing the ferret model based on the technology of utilizing CRISPR / Cas9 on the above aspects, the method can be applied to study human diseases.
Owner:INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES

Method for culturing in-vitro fertilization embryo of oocyte collected from living cow or in vitro

The invention relates to a method for culturing an in-vitro fertilization embryo of an oocyte collected from a living cow or in vitro. The method comprises the following steps: collection and in-vitromaturation of the oocyte, wherein the oocyte can be collected in vitro or from the living cow; in-vitro fertilization, wherein mature COCs is cultured in a fertilization culture solution and then themature COCs and sperm suspension are subjected to co-incubation culture; and in-vitro culture of the embryo and cryopreservation in liquid nitrogen. The method has the excellent technical effect as shown in specification.
Owner:天津力牧生物科技有限公司

Heterogeneous inseminate system

A heterogeneous inseminate including a first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species for the production of sex-selected embryos and sex-selected offspring.
Owner:XY

Freezing method of pig sperms

InactiveCN102138552AImprove integrityImprove in vitro fertilization (IVF) ratesDead animal preservationAnimal scienceLow density
The invention discloses a freezing method of pig sperms. Unexpectedly, the DNA (deoxyribonucleic acid) completeness and the in vitro fertilization (IVF) rate of unfrozen pig sperms can be obviously improved by using 9(w / v)% of low-density albumin for treatment during the freezing process.
Owner:金一

Split-range embryo culture solution and preparation method thereof

ActiveCN103173403AImprove in vitro growth and developmentImprove qualityEmbryonic cellsBiologyCell cycle
The invention discloses a split-range embryo culture solution and a preparation method thereof, belonging to the technical field of cytology and biology. The split-range culture solution added with three embryotrophic factors comprises a cleavage solution and a blastula solution, wherein the cleavage solution and the blastula are respectively used in a fertilized egg before and after 8-cell cycle. The embryotrophic factors added into the embryo culture solution disclosed by the invention have synergistic effect, so that the defect that the split-range culture solution loses the embryotrophic factors secreted by an embryo is avoided, the fertilized egg has higher embryo stem cell pluripotency during the growth in the culture solution disclosed by the invention, the development situation of the fertilized egg is equivalent to the in-vivo embryo growth, the in-vitro growth and development of the fertilized egg is improved, and the quality of the embryo is improved.
Owner:南京优而生物科技发展有限公司

Oocytes derived from ovarian culture initially containing no oocytes

Ovarian germ-line-competent embryonic stem cells (GLC-ESC) are cultured, either in the presence or absence of a compound having estrogenic activity. The GLC-ESC are either collected prior to specific commitment or are permitted to remain in the culture medium for a time sufficient to develop into oocytes, and the oocytes may be fertilized by adding sperm to the culture medium. The fertilized oocytes may be permitted to develop into embryos, which may be transferred into the uterus of an adult human female or frozen for later use. The invention provides a method for obtaining by in vitro fertilization an embryo that is genetically related to a human female who is not producing oocytes.
Owner:OVASCI

Tubule high-density type method for freezing pig jism and the products thereof

The invention discloses a high density freezing maintain pig seminal fluid method with fistula and product in animal propagation technical domain, which comprises the following steps: collecting fresh seminal fluid; detecting; pre-treating the seminal fluid; defreezing; proceeding assessment procedure of defreeze seminal fluid; diluting with freezing diluting liquid; storing with high density super low temperature with 0. 25mL fistula; stabilizing the defreezing sperm above 0. 5 and average active at 0. 58; reaching the highest at 0. 68; proceeding pig external fertilization with the sperm; getting 54. 3% cleavage ratio and 48. 5% morula developing ratio; meeting one sperm dosage with sperm quantity of one 0. 25mL fistula. This invention possesses larger using latent force and market developing prospect.
Owner:NANJING AGRICULTURAL UNIVERSITY

Method for producing in vitro embryos using lamb superovulated oocytes

InactiveCN102296047AIncrease productivityA large number of collectionsEmbryonic cellsExternal fertilizationBiology
The invention discloses an in vitro embryo production method by lamb superovulation oocytes. The production method provided by the invention is accomplished by the following technical scheme: carrying out in vitro culture on oocytes acquired from lamb body in a maturation medium and maturing into ova, carrying out in vitro fertilization and culture so as to make the ova grow into embryos, and transplanting survived embryos into a surrogate ewe body for breeding. In comparison with the prior art, the method provided by the invention is utilized to adopt the oocytes acquired after the lamb superovulation treatment for the in vitro embryo production and has advantages of large quantity of the acquired oocytes, high production efficiency of embryo and short time for breeding lamb offspring. Therefore, the method provided by the invention has an edge in the application of large scale in vitro embryo production.
Owner:XINJIANG ACADEMY OF AGRI & RECLAMATION SCI +1

Reproductive technology of low dose semen production and in vitro / in vitro fertilization in domestic animals

Artificial insemination of female animals and in vitro fertilization of oocytes with fresh or frozen-thawed semen have been applied to the reproduction of animals. According to the traditional theory and conventional procedures, a great number of sperm cells are needed to ensure a successful fertilization.
Owner:XY

Sperm screening and in vitro fertilization bionic device based on micro fluidic technology

The invention provides a sperm screening and in vitro fertilization bionic device based on micro fluidic technology. The sperm screening and in vitro fertilization bionic device based on micro fluidic technology comprises a bionic device main body; the bionic device main body is composed of a glass sheet layer, a silica gel pipeline layer, and a polydimethylsiloxane layer; a center cell is arranged at the middle part of the silica gel pipeline layer; an inlet cell, a first outlet cell, and a second outlet cell are arranged outside the center cell; the center cell is communicated with the inlet cell, the first outlet cell, and the second outlet cell via a first pipeline, a second pipeline, and a third pipeline respectively; the first pipeline is provided with a first sample adding port; the second pipeline is provided with a second sample adding port; and the third pipeline is provided with a third sample adding port. The sperm screening and in vitro fertilization bionic device is used for stimulating complete natural impregnation process, unfavorable influences of operation on gametes and embryos are avoided, and polyspermy is avoided; high quality embryos at stimulated natural impregnation states can be obtained, in vitro fertilization success rate is increased greatly, the potential causes of sterility can be identified, and a direction is provided for clinical treatment.
Owner:NANJING JINGJING BIOTECHNOLOGY CO LTD

External fertilization and embryo transplantation information checking method

The invention provides an external fertilization and embryo transplantation information checking method. The method comprises the following steps that (1) identity information corresponding to a sperm, an ovum, an embryo and a matrix is stored in a database; (2) relevant identity information of culture dishes which carry the sperm, the ovum and the embryo and relevant identity information of a matrix identity recognition ring are recognized through the RF technology; (3) searching is performed on the database to compare whether the obtained identity information is matched with the database or not, and pairing is correct if yes; if not, pairing is wrong. According to the external fertilization and embryo transplantation information checking method, the defects in the prior art are overcome; under the condition of no manual intervention, the identity information of the culture dishes which carry the sperm, the ovum and the embryo and the identity information of the matrix identity recognition ring are recognized through the RF technology during external fertilization and embryo transplantation, and the recognized identity information is compared. In this way, the accuracy of the external fertilization and embryo transplantation can be guaranteed.
Owner:南京爱贝生物科技有限公司

Culture solution for bovine in vitro fertilization (IVF) and method for improving bovine IVF

The invention relates to a culture solution for bovine IVF and a method for improving bovine IVF. The method for bovine IVF comprises the following steps: washing a mature cumulus-oocyte complex in afertilization culture solution, transferring the mature cumulus-oocyte complex into the fertilization culture solution, and putting the fertilization culture solution into an incubator for later use;taking a frozen seminal tubule from liquid nitrogen, performing unfreezing in a water bath at 37 DEG C, injecting seminal fluid into a centrifuge tube containing a seminal fluid preparation culture solution, and discarding a supernatant after centrifugation; adding the seminal fluid preparation culture solution into the centrifuge tube, resuspending sperm precipitate, and taking a proper sperm suspension for sperm counting; and adding the sperm suspension with the calculated volume into fertilization culture liquid drops containing oocytes, placing a culture tray into the incubator, and carrying out sperm-ovum co-incubation to complete IVF operation for embryo in-vitro culture. The fertilization culture solution and the seminal fluid preparation culture solution contain sodium pyruvate, heparin and other components. The method can significantly improve the efficiency of bovine IVF.
Owner:天津力牧生物科技有限公司

Green transgenic fluorescent ornamental fish

The present invention relates to transgenic green ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
Owner:GLOFISH LLC

Method for bovine in-vitro fertilized embryo culture and used culture solution

The invention relates to a method for bovine in-vitro fertilized embryo culture and a used culture solution. Particularly, on one hand, the invention relates to a method for culturing a bovine in-vitro fertilized embryo. The method comprises the following steps of putting the bovine in-vitro fertilized embryo into a bovine in-vitro fertilized embryo culture solution and carrying out embryo in-vitro culture under the conditions that the temperature is 38.5 DEG C, the content of CO2 is 0.5% and the humidity is 100%. The invention further relates to a bovine in-vitro fertilized embryo culture solution. The culture solution comprises NaCl, KCl, NaHCO3, MgCl2, KH2PO3, sodium pyruvate, glucose, calcium galactonate, fetal calf serum, L-glutamine, an essential amino-acid, a nonessential amino acid, glutathione and water. The method and the used culture solution have excellent technical effects in the specification, for example, the method has a higher blastocyst hatching rate when the bovine in-vitro fertilized embryo is cultured.
Owner:天津力牧生物科技有限公司 +1

Phospholipid compositions and use thereof to enhance spermatozoa motility and viability

Sperm mobility and impregnation of an oocyte is enhanced by placing sperm in an aqueous solution of phospholipids prior to in vitro fertilization or artificial insemination. The aqueous phospholipid solution can also be used during storage or cryopreservation of the sperm. Also, the motility of sperm produced or ejaculated and the environment into which it is placed is enhanced my ingestion of the phospholipid composition by the male or female, or a vaginal placement of compositions containing the phospholipids.
Owner:NUTRITIONAL THERAPEUTICS

In vitro maturation culture method of denuded oocytes of mice

InactiveCN103898048AHigh maturation efficiencyImprove development qualityGerm cellsExternal fertilizationBlastula
The invention discloses an in vitro maturation culture method of denuded oocytes of mice. The method comprises the following steps: (1) performing in vitro maturation culture on the denuded oocytes; (2) performing parthenogenetic activation on matured denuded oocytes; (3) performing in vitro fertilization on the matured denuded oocytes. According to the method, an environment for artificially simulating natural maturation of oocytes can be created, so that the denuded oocytes can efficiently maturate and grow in vitro and can be subjected to in vitro fertilization and embryonic development as same as normally growing oocytes after in vitro maturation culture. The practice proves that the in vitro maturation culture efficiency of the denuded oocytes of the system is high, the system is mature and stable, and the embryonic development quality of the denuded oocytes is high after in vitro natural fertilization, so that the application and popularization of the denuded oocytes in reproductive health of human beings and genetic breeding of modern livestock can be greatly facilitated. In addition, the operation of obtaining blastulae from the denuded oocytes is simplified and the denuded oocytes serving as ovum sources can be conveniently put into actual production.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Method for improving blastocyst rate of in-vitro embryos of animals

InactiveCN102703377AImprove developmental abilityImprove early developmental abilitiesEmbryonic cellsCulture fluidEmbryo
The invention provides a method for improving the blastocyst rate of in-vitro embryos of animals. According to the method, the developmental capacity of a parthenogenetic embryo and an in-vitro fertilization embryo can be remarkably improved by adding type I interferon into an embryonic development culture solution for the in-vitro culture of the embryos; compared with the blastocyst rate obtained under the culture of the conventional culture solution, the blastocyst rate is improved by 7 to 13 percent; and the blastocyst hatching rate is also remarkably higher than the blastocyst hatching rate under the culture of the conventional culture solution. By the method, the early development capacity of in-vitro embryos of cattle and sheep is improved, so that the in-vitro production efficiency of embryos of the cattle and sheep is improved, and high-quality embryos are provided for the scientific research and production field in which the embryos of the cattle and sheep are taken as materials.
Owner:CHINA AGRI UNIV

Two-layer percoll density gradient centrifugal separation method of boar sperms

InactiveCN102250836ASimple processing methodEasy to handleGerm cellsAnti-sperm antibodySemen liquefaction
The invention provides a two-layer percoll density gradient centrifugal separation method of boar sperms. The method is suitable for normal sperm specimen and sperm specimen with low sperm density or low sperm motility, and is a simple, convenient and fast sperm treatment method. The method can be used for remove leucocytes, bacteria, fragments and anti-sperm antibodies in the sperms and improving the delayed sperm liquefaction, the sperm in-vitro capacitation and the like, so that the sperms can effectively fertilize ova to improve the pregnancy rate, and the percentage of the sperms with motility and normal shape in the obtained sperms is about 95%. The method provided by the invention is suitable for screening sperms with motility from fresh boar sperms and diluted sperms, thus providing high-quality sperms for the study of boar sperm capacitation mechanism and in-vitro fertilization.
Owner:JILIN UNIV

In-vitro culture solution for culturing swine parthenogenetic activated embryos and swine in-vitro fertilized embryos

InactiveCN103710299AImprove developmental efficiencyIncrease the number ofEmbryonic cellsCulture fluidEmbryo
The invention discloses a swine in-vitro embryo culture solution. The swine in-vitro embryo culture solution disclosed by the invention is obtained through adding ligustrazine, with the final concentration of 0.05-5.00 microgram / milliliter, into an embryo culture solution NCSU-23. A traditional Chinese medicine monomer component, namely ligustrazine, is added into the swine in-vitro embryo culture solution disclosed by the invention; shown by experiments, through carrying out in-vitro culture on the swine parthenogenetic activated embryos and the swine in-vitro fertilized embryos by using the culture solution, the development efficiency of the embryos and the number of the cell masses and total cells in blastulae can be effectively increased. The swine in-vitro embryo culture solution has the advantages that a foundation is laid for the development of other biotechnologies, medical research and animal husbandry, and meanwhile, a certain theoretical basis for clarifying the development mechanism of early swine embryos is provided.
Owner:BEIJING UNIV OF AGRI

Culturing device, culturing liquid and culturing system for external fertilization of human ovums and sperms and early development of embryo

The invention discloses a culturing device, a culturing liquid and a culturing system for the external fertilization of human ovums and sperms and the early development of an embryo. The culturing device comprises at least two thermostatic culturing chambers, two small-sized air bottle chambers, a mixed gas filtering device, an air pressure protecting device, a mixed gas thermostatic preheating chamber, an air supplying pipe and the like, wherein each culturing chamber is a confined space with the volume of at least 100ml. The culturing liquid contains sodium chloride, magnesium sulfate, monopotassium phosphate, sodium bicarbonate, ethylenediamine tetraacetic acid, taurine, sodium pyruvate, L-lactic sodium lactate, glucose, gentamicin, alanine-glutamine dipeptide, 18 types of L-lactic amino acids except alanine and glutamine, and the like. When the culturing device and the culturing liquid are used, the external fertilization of the human ovums and sperms and the whole process of the development of the embryo before embryo implantation can be finished; the high-quality blastocyst forming rate and the embryo implantation rate are obviously increased.
Owner:余裕炉

Method for improving quality of external fertilization embryo of oocyte of sheep

The invention provides a method for improving quality of an external fertilization embryo of an oocyte of a sheep. The method relates to TrichostatinA (TSA) which is used for treating the oocyte of the sheep and can greatly improve the quality of the external fertilization embryo of the oocyte of the sheep, by adopting the method improving the quality of the external fertilization embryo of the oocyte of the sheep through TSA, after 168 hours, the blastocyst rate and the number of the obtained blastomeres are remarkably higher than those of the matched group, therefore, the method has better scientificity and practicability.
Owner:新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心

Automated Intracytoplasmic Sperm Injection Assisted Fertilization System

An integrated automated system comprising a microfluidic cassette, and methods of use thereof, for intracytoplasmic sperm injection assisted fertilization. The microfluidic cassette and the integrated automated system provides a complete set-up of human gametes for assisted in vitro fertilization, including proper cell stage recognition, gamete propulsion via microfluidic currents, microinjection of a spermatozoon into an oocyte, and subsequent embryo culture and monitoring, thus allowing widespread distribution of in vitro insemination by favoring affordability.
Owner:CORNELL UNIVERSITY

Progestin nano composition and preparation method thereof

The invention relates to a progestin nano composition, an applying pellet containing the progestin nano composition, a method for preparing the progestin nano composition and the applying pellet, preparations containing the progestin nano composition and / or the applying pellet such as capsules, tablets and lyophilized powder, and application of the progestin nano composition and the applying pellet on preparation of medicines. The medicines are used for treating and / or preventing diseases caused by hormone deficiency, or are used for hormone replacement therapy after external fertilization.
Owner:INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A +1

Method for producing sex controllable in vitro embryo of buffalo

The method of producing extracorporeal buffalo embryo in controlled sex includes recovering buffalo egg in butchery or from living buffalo body, extracorporeal culturing to mature, external fertilization with fresh or frozen X or Y sperm of fine breed bull in a flow cytometer; and recovering embryo of the fertilized egg via external culture to blastula stage for transplanting or further freeze maintaining. The embryo is used in generating posterity of the expected sex. The present invention has the advantages of industrial embryo production in controlled sex and raised propagation speed of high yield milk buffalo.
Owner:GUANGXI UNIV

Management control system based on external fertilization-embryo transplantation

The invention relates to a management control system based on external fertilization-embryo transplantation. The system comprises a doctor's advice management module, an electronic medical record module, an identity identification module, a patient management module, a laboratory management module and a heredity management module, in the abovementioned management system, a set of software system containing a big data acquisition and processing technology and a complex embryo transplantation algorithm is also arranged, the software system includes detection data acquisition and processing software deployed in a reproductive center, a software program installed in a computer of an attending doctor and used for automatically calculating a transplantation operation schedule and managing an embryo transplantation process and an APP program installed in an intelligent mobile phone and used for notifying and reminding a patient to transplant. The system efficiently fuses a complicated clinical treatment process and a patient service process, thereby improving a capability of serving patients of the reproductive center, enhancing satisfaction of the patients, and greatly reducing doctor-patient contradiction.
Owner:HUNAN QIFENG TECH CO LTD
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