9 results about "Bovine oocyte" patented technology

The invention discloses a bovine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof, and belongs to the technical field of animal reproduction and embryo engineering. The preparation method of the bovine oocyte in-vitro maturation culture solution comprises the step of adding mitoquinine mesylate into a conventional IVM culture solution, wherein the adding concentration of the mitoquinine mesylate is preferably 50 nmol / L. The bovine oocyte in-vitro maturation culture solution provided by the invention can effectively reduce the oxidative stress reaction of the oocytes in the in-vitro culture process, enhance the mitochondrial function and remarkably improve the bovine oocyte in-vitro maturation quality and the embryonic development potential, so that the blastocyst rate is increased, and the problem that the development potential of the existing oocyte IVM culture solution is insufficient is solved.

The present application relates to the field of animal embryo engineering technology, and provides a method for improving the development ability of bovine IVP embryos. In the production of bovine in vitro embryos, by adding PAPPA, ergothioneine and selenocysteine to the bovine oocyte in vitro maturation culture solution, early embryo culture solution, late embryo culture solution, freezing solution and thawing solution, the in vitro maturation performance of oocytes and the development performance and vitality of the subsequent obtained embryos can be effectively improved. Studies have shown that after adding the above additives, the maturation rate of oocytes, the cleavage rate, the blastocyst rate of embryos, and the freezing survival rate, the hatching rate and the ATP content of embryos after freezing preservation are significantly improved, which has important application value.

The invention discloses a culture solution for improving in-vitro culture blastocyst rate of bovine oocytes and application of the culture solution, and belongs to the technical field of cell in-vitro culture. According to the culture solution, kaempferol is added into a basic culture solution, and the addition concentration of the kaempferol is 0.01-1 mu M / L; preferably, the addition concentration of the kaempferol is 0.1 mu M / L. By optimizing the concentration of the kaempferol and the synergistic proportion of the kaempferol and basic nutritional ingredients, the action mechanism of the kaempferol for regulating and controlling the mitochondrial function of the oocyte and meiosis spindle assembly is defined, and the direct association of'oxidation resistance-development potential improvement 'is established, so that the blastocyst formation rate and embryo quality of in-vitro culture of the oocyte are remarkably improved, and the development potential of the oocyte is improved. The requirements of the fields of assisted reproduction, embryo engineering and the like on an efficient and stable oocyte in-vitro culture technology are met.

The invention discloses a culture solution for promoting in-vitro maturation of bovine oocytes and application of the culture solution, and belongs to the technical field of assisted reproduction. The invention provides the culture solution which is composed of brand new components and is used for promoting in-vitro maturation of the bovine oocytes, support is provided for maturation of the oocytes from multiple aspects of carrier transportation, energy metabolism regulation, signal transduction, extracellular environment and the like, the in-vitro maturation quality of the bovine oocytes can be effectively promoted, and the survival rate of the bovine oocytes is increased. The quality and consistency of nucleus maturation and cytoplasm maturation of oocytes are obviously improved; the cultured mature oocytes are used for in-vitro fertilization, so that the fertilization proportion and the blastocyst hatching rate can be remarkably improved; the provided culture solution is appropriate in component number, clear in proportion, simple and easy to operate in preparation method, short in time consumption, good in repeatability and suitable for batch production, and has a good application prospect in maturation culture of bovine in-vitro oocytes.

The invention relates to the technical field of gene editing, in particular to a composition and a culture solution for promoting development of bovine gene editing cloned embryos and application of the composition and the culture solution. The composition (DLL3, Vercircon and ML604086) is added into an oocyte in-vitro maturation culture medium, and the DLL3, the Vercircon and the ML604086 cooperate with one another, so that apoptosis and autophagy injury of bovine oocytes are effectively reduced, the cleavage rate and blastocyst rate of embryos are increased, and the method has important practical value for improving the production efficiency and quality of gene editing cloned embryos.

The application discloses a one-step freezing solution for bovine in-vitro embryos and a one-step freezing method for bovine in-vitro embryos and belongs to the technical field of biotechnology.The method comprises the following steps: bovine oocyte in-vitro maturation; bovine in-vitro fertilization embryo production; after culturing the bovine in-vitro fertilization embryo for 6-8 days, the embryo is moved into freezing solution A, and after standing, the shriveled blastocyst is moved from the freezing solution A into freezing solution B, the embryo sinking to the bottom of the freezing solution B is moved into freezing solution C, is loaded into a macaroni tube, and is subjected to programmed freezing; after the freezing program is completed, the macaroni tube is put into liquid nitrogen. The bovine in-vitro embryos are frozen by using the three kinds of freezing solutions, on the premise that the conventional freezing method has low technical requirements for the operator and can be directly transplanted after thawing, the conventional freezing method is overcome, the survival rate, the hatching rate and the transplantation pregnancy rate of the embryos after thawing are low, the convenience of use and the effective integration of the high transplantation pregnancy rate are realized.

The invention discloses a method for improving the reproductive performance of cattle by using betaine, and belongs to the technical field of animal reproduction biology. The method comprises applying an effective dose of betaine to bovine oocytes and / or granular cells thereof; the betaine is a natural quaternary ammonium type alkaloid with an anti-oxidation function. According to the invention, it is found through metabonomics that betaine is a key metabolite of cattle high-yield breeding traits for the first time, and it is proved that betaine has double beneficial effects in a cattle breeding system: on a granular cell level, the level of active oxygen in cells induced by hydrogen peroxide can be significantly reduced by adding 5 mM betaine, and oxidative stress can be effectively relieved; on the oocyte level, 0.5 mM betaine is added into an in-vitro maturation and / or embryo culture solution, so that the cleavage rate and blastocyst formation rate of the bovine in-vitro embryo can be remarkably increased. The invention provides a brand-new, safe and effective solution for improving the production efficiency and the reproductive capacity of the bovine in-vitro embryo.

The present application relates to the technical field of gene editing, in particular to a composition for promoting the development of bovine gene editing cloned embryos, a culture solution and application thereof. The present application adds the composition (DLL3, Vercirnon and ML604086) in the in vitro maturation culture medium of oocytes, and effectively reduces the apoptosis and autophagy damage of bovine oocytes through the synergistic cooperation of DLL3, Vercirnon and ML604086, thereby improving the cleavage rate and blastocyst rate of embryos, which has important practical value for improving the production efficiency and quality of gene editing cloned embryos.

The invention provides a method for improving the production efficiency of bovine in-vitro embryos, and belongs to the technical field of livestock breeding biology. According to the method, carnosine is added in the bovine oocyte in-vitro maturation stage, melatonin is added in the embryo culture stage after in-vitro fertilization, and the embryonic development effect is remarkably improved through the staged synergistic effect. According to the method, the characteristics of energy metabolism regulation and control of carnosine and oxidation resistance of melatonin are fully utilized, the defects of oxidative stress and insufficient energy supply in in-vitro culture are effectively overcome, the blastocyst development rate and the embryo quality are improved, and the method is high in safety, easy and convenient to operate and good in application prospect.