52 results about "Motile sperm" patented technology

A method for measuring the total sperm concentration (TSC) in a sample is described and includes: (i) placing the sample in a transparent container between a synchronically pulsed light source and a photodetector; and (ii) measuring the optical absorbance of the sample in the range of 800-1000 nm, the TSC of the sample being proportional to the absorbance. Also described is a sampling device for use in optically analyzing a biological fluid, a method for measuring motile sperm concentration (MSC) in a semen sample, a method of determining the average velocity (AV) of sperm cells and a system for analyzing semen quality including means for measuring TSC, means for measuring MSC; and a video visualization system.

The invention relates to a raman spectrum-based fast measurement method for a motile sperm deoxyribonucleic acid (DNA). The invention adopts the following technical scheme: collecting a semen sample by a test tube; naturally liquefying semen in a room-temperature environment; absorbing the liquefied semen and adding to a centrifugal tube containing density gradient centrifugate; absorbing upper seminal plasma after centrifuging, discarding, then adding a PBS buffer solution and centrifugally washing again; absorbing supernatant, discarding, adding the PBS buffer solution to prepare a sperm suspension, and incubating; attaching a metal bottom plate to a culture dish; adding the PBS buffer solution, and absorbing the incubated sperm suspension, soaking in the culture dish, wherein the sperm swims outside and contacts the metal bottom plate; setting a spectrum response range, integrating time and the excitation light wavelength, so as to obtain raman spectrum data from the head part of the sperm. The motile sperm swimming out from the pointed-end part of a sucker can be attached to the metal bottom plate by operation of a thin tube pipette, and the damage condition of the sperm DNA is evaluated by measuring the sperm of which the head is attached to the metal bottom plate.

Motile particles are sorted from non-motile particles in a microfluidic sorting device wherein a stream of sort fluid containing motile and non-motile particles is caused to flow adjacent a media stream in non-turbulent fashion through a sort channel, during which flow motile particles cross the interface between the adjacent flow streams, entering the media stream, and forming a motile particle-depleted sort stream. The sorting devices are easily and inexpensively fabricated and have numerous uses, in particular sorting of motile from non-motile sperm.

A microchip includes a first chamber and second chamber formed to a predetermined depth in a member made of polydimethylsiloxane (PDMS); and a channel (microchannel) connecting the first chamber to the second chamber. These components mimic in vivo organs which produce by selecting capacitated motile sperm from semen and causing the selected sperm to meet mature oocyte, and these components are capable of effectively producing motile capacitated good-quality sperms from sperms ejaculated outside the body.

The present disclosure relates to a method of extracting high purity polydeoxyribonucleotide (PDRN) from a salmon testis, the method comprising: 1) separating seminal fluid and an immature testis region from the salmon testis, 2) mildly grinding the immature testis region and then diluting with artificial seminal plasma, 3) treating the diluent with a predetermined concentration of human chorionic gonadotropin (hCG), and 4) extracting the high purity polydeoxyribonucleotide (PDRN) from the salmon testis. The method comprises the following steps: (1) extracting sperm from a sperm diluent to induce artificial maturation of the sperm cells into sperms, (4) centrifuging the sperm diluent and collecting potential active sperms after hCG treatment for a preset time, and (5) extracting PDRN from the collected sperms. According to the method, 100-200 times of PDRN raw materials with higher purity can be extracted from 20 mL (maximum 50 mL) of sperms (seminal fluid) collected by salmons at a time. In addition, the method enables the extraction of PDRN in high yield in a cost-effective and economically feasible manner.

The invention discloses a sperm quality assessment device. The sperm quality assessment device includes a main frame and a test pad. The test pad is encircled by the main frame and includes an exposed introductory portion. The test pad has greater hydrophilicity than the main frame, and includes an MTT reagent. Therefore, the activity pattern of the colored motile sperms can be used to estimate sperm quality, such as motility. The present device has the particular advantage of reducing manufacturing cost by using paper, and is also easy to use.

The present invention discloses method and apparatus for regulating optimum flow of semen and separating motile sperms. The device consists of; a first cylindrical shape container, comprising a first external diameter, second external diameter, a thirds external diameter and a mesh, wherein said mesh is comprised of plurality of pores and attached to lower bottom of said first cylindrical shape container; a second cylindrical shape container comprising an internal diameter and an external diameter, wherein said second cylindrical shape container is closed ended at bottom, and embraces said first cylindrical shape container at said second external diameter of said first cylindrical shape container, thereby creating at least a first predetermined distance and at least a second predetermined distance, wherein said at least a first predetermined distance regulates air coming from said second cylindrical shape container, thereby regulating flow of semen and separating motile sperms coming through said mesh to said second cylindrical shape container.