The application relates to the field of
animal breeding technology, in particular to a method and
system for detecting total live
sperm count of a cow and application. The method comprises the following steps: based on the
genome version ARS-UCD1.2, the
methylation level of a region such as chr17:62205563-63205744 of a to-be-detected cow is detected; the detection result is compared with a reference standard, and the total live
sperm count of the to-be-detected cow is obtained; the reference standard is obtained based on GWAS correlation data of the total live
sperm count and the
methylation level of a reference cow group in the region of chr17:62205563-63205744. A region significantly related to the total live sperm count of the cow is obtained through research, early identification of the total live sperm count of the cow can be realized by detecting the
methylation level of the region, and this has important significance in the field of animal
phenotype identification.