15 results about "Motile sperm" patented technology

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.

Systems for sorting sperm include a housing and a microfluidic system supported by the housing. The systems also include two or more inlets providing access to the microfluidic system to deliver sperm or semen and fluid to the microfluidic system, as well as an outlet for harvesting sorted sperm. The systems include a flow channel that provides a flow path for sperm from an inlet to an outlet while sperm travels against a fluid flow towards an outlet for harvesting. In the systems, fluid delivered to the microfluidic system via an inlet flows from the inlet towards one or more outlets, and the flow channel extending along the length of the microfluidic system provides a flow path for motile sperm to travel against the fluid flow towards a collection outlet for harvesting. In some embodiments of a system, the microfluidic system also includes a microporous filter arranged in the flow path between the inlet and the collection outlet to cause motile sperm traveling against the fluid flow to pass through the filter to reach the collection outlet for harvesting.

The sperm optimal tube comprises an upper tube, a lower tube and an inner tube, the upper tube and the inner tube are communicated, the upper tube is detachably connected with the lower tube, when the upper tube is connected with the lower tube, the lower tube is located outside the inner tube, one end of the inner tube is communicated with the upper tube, and the other end is provided with an opening allowing sperm to pass through, and a switch valve is arranged on the opening, the switch valve can be opened under the action of centrifugal force and closed after the centrifugal force stops. The device can effectively separate normal and active sperm, and especially because the switch valve is arranged, the sperm is allowed to flow out under the action of centrifugal force, and the switch valve is closed to seal the inner tube when the centrifugal force is eliminated, so that the abnormal sperm, the inactivated sperm and other cell components in the semen in the inner tube are prevented from entering the lower tube, and the complete separation of sperm with different activities is realized; the design of the inner tube, the upper tube and the lower tube facilitates the disassembly of the outer tube, the semen is more convenient to take out, and the mutual interference is avoided.

The application discloses a high-precision sperm morphology screening system and a screening method, and relates to the technical field of sperm cell recognition. Raw semen is obtained and filtered to obtain initial semen; the initial semen is spread into a preset single-layer sperm liquid strip to obtain initial single-layer motile sperm; a first magnification operation is performed on the initial single-layer motile sperm to obtain a first initial single-layer motile sperm image; a second magnification operation is performed on the first initial single-layer motile sperm image to obtain a second initial single-layer motile sperm image; and the second initial single-layer motile sperm image is substituted into a high-precision sperm morphology selection model to obtain target high-quality sperm. Through the preliminary filtration of the raw semen and the secondary screening of the preset single-layer sperm strip spreading method, and by adopting the progressive magnification strategy of the first magnification operation and the second magnification operation, the high-precision sperm morphology screening can be performed when high-quality sperm is selected by using the existing IMSI technology, and the screening efficiency of the high-precision sperm is improved.

Disclosed is a device for collecting motile sperm, the device comprising a post having a top, a bottom, and a wall, the wall comprising a mesh layer and a membrane layer, an inlet disposed at the top of the post, and a nose disposed at the bottom of the post.

A system and method for improving sperm selection during in vitro fertilization (IVF) procedures includes a sperm containment apparatus equipped with a capture system designed to isolate motile sperm as they respond to the presence of a retrieval instrument within the sperm solution. As more motile and potentially healthier sperm move away from the retrieval instrument, they are selectively corralled within certain regions of the apparatus. The capture mechanism may include various structures, such as a barrier member including one or more passageways which direct sperm movement. By isolating sperm based on their motility, this invention enhances the selection process, improving the chances of successful fertilization with higher-quality sperm. This system provides a more efficient and targeted approach to sperm retrieval compared to conventional methods.

A device for filtering out non-functional sperm and separating motile sperm may include a main channel having a flow direction from a first end toward a second end of the main channel. The device may include a buffer inlet in fluid communication with the first end of the main channel. The apparatus may include a collection chamber in fluid communication with the second end of the main channel. The device may include a secondary channel in fluid communication with the collection chamber on a downstream side. The apparatus may include a waste chamber in fluid communication with the secondary channel on a downstream side.

The application relates to the field of animal breeding technology, in particular to a method and system for detecting total live sperm count of a cow and application. The method comprises the following steps: based on the genome version ARS-UCD1.2, the methylation level of a region such as chr17:62205563-63205744 of a to-be-detected cow is detected; the detection result is compared with a reference standard, and the total live sperm count of the to-be-detected cow is obtained; the reference standard is obtained based on GWAS correlation data of the total live sperm count and the methylation level of a reference cow group in the region of chr17:62205563-63205744. A region significantly related to the total live sperm count of the cow is obtained through research, early identification of the total live sperm count of the cow can be realized by detecting the methylation level of the region, and this has important significance in the field of animal phenotype identification.

A sperm sorting device is suitable for sorting a plurality of motile sperm from a semen sample, and includes a base and a first sorter positioned on the base. The base includes a receiving space defined by a bottom wall and a surrounding wall. The first sorter includes a first kit and a first permeable membrane. The first kit has a first sleeve for being placed into the receiving space. The first sleeve has a first inner sleeve surface and a first outer sleeve surface. The first permeable membrane is fixed to the first sleeve. The first permeable membrane and the inner wall surface of the bottom wall and the surrounding wall define a first sample receiving chamber for receiving the semen sample. In this way, the motile sperm can naturally swim upward and pass through the first permeable membrane, and the quality of sperm sorting can be improved.

This invention discloses a barrel-shaped sperm selection device for selecting highly motile sperm from semen samples. The device includes a container with a porous membrane inside, which divides the interior of the container into an initial chamber and a filtration chamber. This allows the semen sample to move through the porous membrane to the filtration chamber, achieving the selection purpose. The advantages of this invention are: the overall barrel shape of the device, with the porous membrane arranged on a hub along the axial direction of the cylindrical container to form inner and outer cavities, simplifies the structure and optimizes the principle, greatly reducing the requirements for molds and processes. The barrel-shaped structure with inner and outer cavities prevents semen overflow even when the semen sample volume is too large; and prevents reduced selection rates due to air bubbles even when the semen sample volume is too small.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.

Systems and methods for male fertility analysis may include: loading an aliquot of a male fertility sample into a chamber of an analysis system via an inlet, the chamber having an opening covered over by a porous membrane and a concave bottom opposite the opening; introducing media so that the membrane acts as a migration path for motile sperm; removing the media and a motile portion of the aliquot from the system into a container; sealing the container; and transporting the sealed container and a remainder of the male fertility sample to a testing facility. The testing facility may: determine a first concentration of the portion of the sample from the first container; determine a second concentration of the remainder; and estimate a percentage of total motile sperm for the sample based on the first concentration, the second concentration, and a predetermined relationship between portions and remainders of samples.

A method for enhancing artificial insemination (AI) in mammals, particularly bovine livestock, uses a specialized aqueous mastic solution to substantially increase conception success rates from conventional 60-65% to about 95%. The solution comprises ionic minerals including magnesium chloride, calcium chloride, sodium, potassium, and an emulsion of milled mastic gum (Pistacia lentiscus, preferably Chios Mastiha, particulates 15-40 μm) dissolved in water sourced from inland saltwater bodies such as the Great Salt Lake, delivering approximately 72 ionic and trace minerals. The process involves: preparing the uterus by depositing the solution via syringe to maintain pH at 7.35-7.45 and eliminate bacteria; washing donor semen in the solution to isolate healthy, motile sperm and remove debris / reactive elements; and depositing the washed semen directly into the uterus during ovulation, confirmed via luteinizing hormone detection, ultrasound, or chorionic gonadotropin induction.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.

The invention discloses a high-precision sperm morphology screening system and screening method, and relates to the technical field of sperm cell recognition. Obtaining original semen and filtering to obtain initial semen; paving the initial semen into a preset single-layer sperm liquid strip to obtain initial single-layer motile sperms; performing a first amplification operation on the initial single-layer moving sperm to obtain a first initial single-layer moving sperm image; performing second amplification operation on the first initial single-layer moving sperm image to obtain a second initial single-layer moving sperm image; and substituting the second initial single-layer moving sperm image into a high-precision sperm morphology selection model to obtain a target high-quality sperm. According to the sperm morphological screening method, primary filtration of original sperm, secondary screening of a preset single-layer sperm strip laying method and a progressive amplification strategy of the first amplification operation and the second amplification operation are adopted, so that the problem that high-precision sperm morphological screening can be performed when high-quality sperms are selected by the existing IMSI (International Mobile Subscriber Identity) technology is solved, and the screening efficiency of the high-precision sperms is improved.