31 results about "Azoospermatism" patented technology

The invention relates to research of a human azoospermia and severe oligozoospermia detection method, and provides a rapid detection method for researching human azoospermia and severe oligozoospermia. The method includes the steps: acquiring samples; treating the samples; performing diagnosis by the aid of a real-time PCR (real-time polymerase chain reaction) platform; performing ELISA (enzyme-linked immuno sorbent assay) detection by the aid of sample treating results. The invention further provides a kit using the method. The kit comprises RNAs (ribose nucleic acid) and tissue metabolites, wherein the RNAs are extracted after the samples are treated. The research method helps patients suffering from the human azoospermia and the severe oligozoospermia to be rapidly detected and diagnosed, and the kit can help the patients to rapidly find pathogenesis for treatment.

A sperm-producing decoction is disclosed and warms and invigorates kidney yang. The decoction is used for patients with male infertility caused by insufficiency of the kidney yang, low sperm count, azoospermia, or a sperm amount lower than a normal value, and low sperm motility. A traditional Chinese medicine composition is prepared from following raw material medicines by weight: prepared rehmannia root, Chinese wolfberry, raspberry, common dodder, pulp of dogwood fruit, fruit of magnoliavine, semen plantaginis, epimeddium and morinda root. The sperm-producing decoction has good effects for azoospermatism, oligospermia, and normal sperm count type infertility, and is good in effects and quick-acting in curative effects for male infertility. Natural traditional Chinese components are free of side effects for human bodies. The sperm-producing decoction has functions of strengthening sperms and effectively increasing the amount of sperms.

The invention belongs to the technical field of traditional Chinese medicines, in particular discloses a Chinese medicinal composition for treating necrospermia, asthenospermia oligospermia, or aspermatism, which comprises the following raw material medicaments in part by weight: 10 to 20 parts of epimedium brevicornum, 10 to 20 parts of medicinal indianmulberry root, 10 to 20 parts of desertliving cistanche, 10 to 20 parts of south dodder seed, 10 to 20 parts of siberian solomonseal rhizome, 10 to 20 parts of Chinese angelica, 7 to 13 parts of common curculigo rhizome, 8 to 16 parts of Chinese thorowax root, 8 to 16 parts of large-headed atractylodes rhizome, 14 to 26 parts of astragalus, 14 to 26 parts of radix rehmanniae praeparata, 14 to 26 parts of dioscorea root, 3 to 8 parts of liquorice root, 5 to 13 parts of glossy privet fruit, 10 to 20 parts of plantain seed, 10 to 20 parts of palmleaf raspberry fruit, 20 to 40 parts of root of red-rooted salvia, 8 to 16 parts of eucommia bark, 7 to 13 parts of baicalin, 3 to 8 parts of salivia chinensis, 6 to 13 parts of Indian buead and 6 to 14 parts of oriental waterplantain rhizome. The Chinese medicinal composition has the effects of warming and invigorating kidney yang with tonifying qi and invigorating blood circulation and supplementing essence and replenishing marrow, and has the advantages of simple preparation, convenient administration, good curative effect and no toxic or side effect.

The invention provides application of a serum miRNA biomarker, and relates to biomarkers. The serum miRNA biomarker comprises at least one of miR-34c, miR-1275, miR-375, miR-410 and miR-758. The serum miRNA biomarker can be applied to preparation of a reagent for detecting oligospermia and azoospermia. A serum miRNA biomarker composition comprises at least one of the miRNA and sequences of reverse transcription primers, forward primers and reverse primers of each miRNA, and can be applied to preparation of a reagent for detecting oligospermia and azoospermia. The determined close correlation between the miRNA combination and indexes of oligospermia and azoospermia cases can be used as the biomarker to screen and diagnose patients with oligospermia and azoospermia, and an efficient basis is provided to clinical individual intervention treatment.

The invention discloses a separation and culture method and application of human testis mesenchymal stem cells. The separation method comprises the steps that an obstructive azoospermia patient testis specimen which is processed through tissue biopsy separation is digested with type IV collagenase, digested cell suspension is filtered to remove tissue clumps, and human testis cells are obtained; the human testis cells are marked with a CD51 antibody, separation is performed with a flow cytometer, the cells expressing the CD51 are collected, and then the human testis mesenchymal stem cells with the positive CD51 are separated. The invention further provides the human testis mesenchymal stem cells which are obtained through the separation method and have the positive CD51, the culture method of the stem cells and application of the stem cells in preparation of medicine for treating related diseases caused by low testosterone level.

The invention relates to a biomarker, and particularly relates to a serum miRNA biomarker composition and an application thereof. The serum miRNA biomarker composition comprises at least one miRNA and sequences of reverse transcription primers, forward primers and reverse primers of all miRNAs. The serum miRNA biomarker composition can be used for preparing oligozoospermia and azoospermia detection reagents. An oligozoospermia and azoospermia detection kit comprises the serum miRNA biomarker composition, Taq enzyme, dNTP, MgCl2 and a PCR buffer solution. The serum miRNA biomarker composition provided by the invention has the advantages of high sensitivity, low sample consumption, wide detection range and wide linear quantitative range.

The invention relates to a preferential sperm freezing culture device which comprises freezing slide glass, a sperm tank, a sperm swimming channel, a collecting tank and a blocking piece. The volume of the sperm tank ranges from 50 microliters to 500 microliters, the volume of the collecting tank ranges from 5 microliters to 20 microliters, the width of the sperm swimming channel ranges from 10 micrometers to 30 micrometers, and the sperm swimming channel is connected with the sperm tank and the collecting tank. The preferential sperm freezing culture device has the advantages that the preferential sperm freezing culture device is applicable to screening preservation of frozen sperm, motile sperm can be automatically separated by the design of the sperm tank, the sperm swimming channel andthe collecting tank, operation amount is decreased, mechanical damage to the sperm caused by manual picking is avoided, the proportion of usable motile sperm is increased, the freezing slide glass ofall existing sperm can be completely replaced, the preferential sperm freezing culture device can be applied to the fields of normal sperm optimization sperm periodic IVF (in-vitro fertilization), ICSI (intracytoplasmic sperm injection) or artificial insemination, sperm collection of oligospermia and asthenospermia patients, surgical sperm extraction of azoospermatism patients, sperm collection and the like, and has a wide popularization and application prospect.

The invention provides a spermatogenic pill having the functions of warming yang, tonifying kidneys and strengthening the essence. The spermatogenic pill is mainly used for treating azoospermatism. The spermatogenic pill has excellent treatment effect for patients having spermatogenic cells without vas deferens obstruction. The traditional Chinese medicine composition is prepared from the following more than twenty medicinal raw materials by weight: herba epimedii, semen cuscutae, the fruit of Chinese wolfberry, desertliving cistanche, fish glue, goat testicle, male silk moth, human placenta, semen allii tuberose, red ginseng, fleece-flower root, rehmannia glutinosa, rhizoma curculiginis, rhizoma curculiginis, fructus psoraleae, cinnamon, cooked monkshood, angelica sinensis, the root of red-rooted salvia and the like. The spermatogenic pill targets man sterility, has the functions of warming yang, tonifying kidneys and strengthening the essence and is mainly used for treating azoospermatism. The spermatogenic pill can take effect quickly for patients having spermatogenic cells without vas deferens obstruction. Natural Chinese herbal medicines does not have side effects on the body. The formula has the functions of strengthening the essence and effectively increasing the number of sperms.

The invention discloses an NANOS2 marker for predicating or diagnosing azoospermia or oligospermia. The NANOS2 marker can be DNA or mRNA of NANOS2 or protein coded by the NANOS2. The invention furtherprovides an NANOS2 polyclonal antibody, and an in vitro diagnostic reagent prepared according to the NANOS2 marker. The in vitro diagnostic reagent is used for predicating or diagnosing azoospermia or oligospermia, has the advantages of high convenience and rapidness, has high accuracy and specificity, can be applied to large-scale screening, can further serve as a clinical detection means, and particularly provides a novel approach for the diagnosis and detection of SCO azoospermia.

The invention discloses an application of a serum miRNA combination as molecular marker for assessing non-obstructive azoospermia. The serum miRNA combination includes hsa-miR-1263, hsa-miR-221-5p, hsa-miR-483-3p, hsa-miR-4275 and hsa-miR-194-3p. Through the miRNA combination, a reagent kit for assessing the non-obstructive azoospermia is prepared. The serum miRNA for assessing the non-obstructiveazoospermia is obtained, further a detection reagent kit containing primers used for quantitative RQ-PCR detection of the serum miRNA combination is obtained, detection is performed through the detection primers and the detection reagent kit, the operation is simple, the accuracy is high, and the serum miRNA combination is worth of extensive popularization.

The invention discloses a drug for treating prostate diseases, erectile dysfunction, premature ejaculation and sterility and a reparation method of the drug. The drug contains the following raw materials in parts by weight: 45-55 parts of rhizoma curculiginis, 45-55 parts of herba epimedii, 90-110 parts of actinolite, 8-12 parts of carthamus tinctorius, 18-22 parts of honeycomb, 8-12 parts of malesilk moth, 18-22 parts of wolfberry, 25-35 parts of pimoinella candolleana and 13-17 parts of cassia twig. The preparation method comprises the following steps: adding water which is 6-8 times of thetotal mass of the raw material drugs into the raw material drugs, boiling with high heat, continuing to carry out decoction for 25-35 minutes with soft fire, and extracting a decoction solution; adding water which is 5-7 times of the total mass of the medicine dreg into the medicine dreg, boiling with high heat, continuing to carry out decoction for 20-30 minutes with soft fire, combining two decoction solutions, and uniformly mixing so as to obtain the drug, or preparing medical wine by virtue of a conventional method. The drug is used for treating male diseases of prostate diseases, erectile dysfunction, premature ejaculation, sterility and the like caused due to oligospermia, low survival rate and azoospermia and has the characteristics of high effect taking speed, good treatment effect, safety and no toxic and side effects. The total effective rate of the drug is 95% proved by being clinically taken by 200 patients.

The Chinese medicine preparation for treating azoospermatism has the recipe comprising pilose antler, deer gelatin, red ginseng, astragalus root, wolfberry fruit and other 11 kinds of Chinese medicinal materials in certain weight proportion. The present invention has the functions of nourishing Yin, invigorating kidney, promoting blood circulation to disperse blood clots, complementing Yin and Yang, dredging meridian and producing sperm. The clinical application shows that the Chinese medicine preparation may be used widely in the treatment of maleí»s azoospermatism caused sterility.

The invention provides medicine for treating male infertility and a preparation method thereof. Male infertility refers to infertility caused by male elements, aetiological agents of the disease are complex, and plenty of diseases or elements can cause male infertility. Clinically, male infertility is categorized into sexual dysfunction and normal sexual function, and the normal sexual function condition can be further divided into such symptoms as azoospermatism, oligospermia, asthenozoospermia and asthenospermia. The medicine is prepared by combining cynomorium coccineum, corn cervi pantotrichum, yam rhizomes, human placenta, white atractylodes rhizome, vigna umbellata, radix angelica sinensis, morinda officinalis, rush, epimedium herbs, rhizoma cibotii, fructus psoraleae, fructus alpiniae oxyphyllae, flatstem milkvetch seeds, exocarpium benincasae, actinolite, polygonum multiflorum, adenophora roots, dendrobe, semen allii tuberosi, sealwort, Chinese wolfberry fruits, mulberries, glossy privet fruits, black sesame, seal penes, prepared rehmannia roots, cistanche, gekko, radix paeoniae alba, lily, radix ophiopogonis and radix polygonati officinalis. All drugs are combined, so that all the drugs achieve a synergistic effect in efficacy, and thereby male infertility can be effectively treated.

The invention belongs to the technical field of medical detection, and discloses a specific marker screening method for detecting azoospermia and severe oligospermia. The method comprises the following steps: collecting a sample, treating the sample, and screening out specific markers of the severe oligospermia and azoospermia; in a testicular tissue level, screening expression of differential microRNA in testicular tissues of normal males and sterile males by using a chip method; in a seminal plasma layer, searching differential marker proteins of normal males and sterile males by using a proteomics method; in the urine level, employing metabonomics for screening the difference of metabolites in urine of normal men and infertility men; integrating data obtained from three high-flux layers, namely a testis tissue layer, a seminal plasma layer and a urine layer, and carrying out correlation analysis on the data and sperm quality, density and vitality; and optimizing and establishing a diagnosis technology. The method is long in detection time, high in efficiency and capable of explaining the cause of dyszoospermia of the patient.

The invention discloses a preparation method and application of a chemiluminiscence detection kit for quantitatively detecting TEX101 concentration, belongs to the field of in-vitro diagnostic reagents, and provides a TEX101 chemiluminiscence detection kit by researching TEX101 concentration difference results of obstructive azoospermia OA and non-obstructive azoospermia NOA. The kit can rapidly and quantitatively detect the concentration of TEX101 in seminal fluid, the sensitivity of identifying obstructive azoospermia OA and non-obstructive azoospermia NOA is 73%, the specificity is 100%, and the kit has a good prediction value for assisted reproduction results.

The invention relates to the field of animal models, in particular to a construction method and application of an azoospermia mouse model. According to the construction method of the azoospermia mouse model, a Pus7 gene on a mouse genome is knocked out. The important role of the Pus7 (Pseudouridine synthase 7) gene in the spermatogenesis process of mice is disclosed for the first time, male mice knocked out by the Pus7 have testicular dysplasia and azoospermia, and a mechanism of regulating testicular development by the Pus7 is expected to provide a new thought for research of male infertility mechanisms.

The invention provides a decoction for promoting fertility and nourishing kidney. The decoction has functions of nourishing kidney and yin and warmly invigorating kidney yang and can be mainly used for treating male infertility caused due to deficiency of kidney yin and yang, gonacratia or spermatorrhea, rare semen, oligospermia and poor sperm motility. Traditional Chinese medicine composition of the decoction is prepared from the following raw materials according to weight: prepared rhizome rehmannia, pulp of dogwood fruit, semen cuscutae, radix achyranthis bidentatae, tortoise plastron and antler pieces. The decoction is excellent effect of treating azoospermatism, oligospermia and infertility with normal sperms, is excellent in effect of treating male infertility and can become effective quickly. The decoction is prepared from natural traditional Chinese medicine components, so that the decoction is free of side effect; the formula of the decoction has the functions of strengthening sperms and effectively increasing the number of the sperms.

The invention provides a stem cell exosome preparation for preventing and treating male sexual dysfunction, the active ingredient of the stem cell exosome preparation is obtained by loading cryptotanshinone on exosome derived from mesenchymal stem cells, and the exosome is loaded with cryptotanshinone through an electrotransfection method. In the process of researching the mesenchymal stem cell exosome in the treatment of non-obstructive azoospermia, a researcher finds that the effect of using the mesenchymal stem cell-derived exosome alone was not obvious, but unintentionally finds that the effect is obviously enhanced after cryptotanshinone is loaded into the exosome through the electrotransfection method; in addition, the researcher also finds that different concentrations of exosomes loaded with cryptotanshinone have different effects.

The invention belongs to the technical field of traditional Chinese medicines, in particular discloses a Chinese medicinal composition for treating necrospermia, asthenospermia oligospermia, or aspermatism, which comprises the following raw material medicaments in part by weight: 10 to 20 parts of epimedium brevicornum, 10 to 20 parts of medicinal indianmulberry root, 10 to 20 parts of desertliving cistanche, 10 to 20 parts of south dodder seed, 10 to 20 parts of siberian solomonseal rhizome, 10 to 20 parts of Chinese angelica, 7 to 13 parts of common curculigo rhizome, 8 to 16 parts of Chinese thorowax root, 8 to 16 parts of large-headed atractylodes rhizome, 14 to 26 parts of astragalus, 14 to 26 parts of radix rehmanniae praeparata, 14 to 26 parts of dioscorea root, 3 to 8 parts of liquorice root, 5 to 13 parts of glossy privet fruit, 10 to 20 parts of plantain seed, 10 to 20 parts of palmleaf raspberry fruit, 20 to 40 parts of root of red-rooted salvia, 8 to 16 parts of eucommia bark, 7 to 13 parts of baicalin, 3 to 8 parts of salivia chinensis, 6 to 13 parts of Indian buead and 6 to 14 parts of oriental waterplantain rhizome. The Chinese medicinal composition has the effects of warming and invigorating kidney yang with tonifying qi and invigorating blood circulation and supplementing essence and replenishing marrow, and has the advantages of simple preparation, convenientadministration, good curative effect and no toxic or side effect.

The invention relates to a method and a kit for diagnosing male infertility caused by Piwil1 gene mutation. The invention discloses a gene related to male infertility such as azoospermia, namely the Piwil1 gene. Moreover, the fact that mutation at multiple positions of the Piwil1 gene can cause the occurrence of male infertility is identified. Thus, the Piwil1 gene can be used as a target gene fordiagnosing male infertility, and also can be applied to the development for the treatment medicines effective for the diseases. According to the technical scheme, explanation is given for the mechanism that PIwIl1 participates in the histone substitution by protamine for the first time.

The invention discloses application of maca and maca polysaccharide in treating testis spermatogenesis disorders, and particularly relates to the application of the maca and the maca polysaccharide intreating the spermatogenesis disorders including oligospermia, asthenozoospermia and azoospermia, especially azoospermia caused by medicine. According to the application, an azoospermia model of a busulfan-induced mouse is used for verifying the improvement effect of the maca and the maca polysaccharide on the busulfan-induced azoospermia mouse.

The invention discloses pathogenic mutation of genetic gametogenesis disorder and a detection reagent thereof. The invention relates to a mutant Pof1b gene, the mutant Pof1b gene is located in a chromosome X and is deletion mutation c.312_316delp.S104fs, the gene number of the wild type Pof1b gene in an Ensemble database is ENSG00000124429, the physical position of the deletion mutation Pof1b geneis a third exon, five bases TACAT are deleted at chrX-84622737-84622742, and following sequences cause frameshift mutation. The mutant Pof1b gene or the mutant POF1B protein is used as a detection target to be applied to preparation of an auxiliary diagnosis reagent or detection equipment for male non-obstructive azoospermia or female hereditary primary ovarian insufficiency.

The invention discloses application of gene SOX30 in the treatment of non-obstructive azoospermia. The invention confirms the relation between the SOX30 and the non-obstructive azoospermia, so that the SOX30 can be used as the medicine, medicine target or target gene in gene therapy to apply to the treatment and relieving of the non-obstructive azoospermia and can provide a new strategy for the treatment and relieving of the non-obstructive azoospermia.

The invention provides a novel estrogen analogue as well as a preparation method and application thereof, and the preparation method comprises the following steps: sequentially adding weighed arylamine compounds, substituted acetylene and a metal catalyst MmXn into a reaction flask, adding a corresponding solvent, reacting in an air atmosphere at the temperature of 60-120 DEG C, stirring for 1-48 hours, cooling to room temperature, adding ethyl acetate for dilution, washing with a saturated saline solution, drying an organic phase with anhydrous magnesium sulfate, and carrying out column chromatography separation to obtain the estrogen analogue. The estrogen analogue is efficiently and rapidly synthesized by adopting a cheap metal catalysis technology; the novel estrogen analogue has an obvious spermatogenesis promoting effect on azoospermia rats, and has the potential of being developed into a specific azoospermia treatment medicine.

In this application, the methylation status of SOX30 gene and the expression levels of mRNA and encoded protein in normal control OA and NOA punctured testis tissues were detected. The detection results showed that SOX30 gene was specifically hypermethylated and expressed silently in NOA punctured testis tissues. Based on the above test results, this application provides a diagnostic box for non-obstructive azoospermia, which can help clinical NOA patients formulate effective treatment plans to improve accuracy and treatment progress, and at the same time help to better understand the cause of NOA and formulate risks. Evaluation and early diagnosis are of great significance.

The invention relates to an application of a C12orf40 gene. In clinical scientific research and practice, the inventor screens and identifies a disease-causing gene C12orf40 cleavage site variation (NM001031748.4, c.1286 + 1Ggt; a), no haploid sperms are found in testis biopsy tissues of a patient, and non-obstructive azoospermia is shown. The gene is specifically expressed in testis tissues, is not expressed in other tissues, can be used as a biomarker of the non-obstructive azoospermia, and is beneficial to further research on a mechanism of the non-obstructive azoospermia and exploration on treatment of the non-obstructive azoospermia.

The Chinese medicine preparation for treating azoospermatism features its recipe comprising ophiopogon root, white peony root, calamus, albizzia, tuckahoe and other nine kinds of Chinese medicinal materials in certain weight proportion. The Chinese medicine preparation of the present invention has the functions of replenishing essence, benefiting kidney, tranquilizing, invigorating spleen, promoting urination, regulating Yin-Yang, etc, and may be used widely in treating maleí»s spermacrasia causing sterility.