31 results about "Azoospermatism" patented technology

The invention relates to research of a human azoospermia and severe oligozoospermia detection method, and provides a rapid detection method for researching human azoospermia and severe oligozoospermia. The method includes the steps: acquiring samples; treating the samples; performing diagnosis by the aid of a real-time PCR (real-time polymerase chain reaction) platform; performing ELISA (enzyme-linked immuno sorbent assay) detection by the aid of sample treating results. The invention further provides a kit using the method. The kit comprises RNAs (ribose nucleic acid) and tissue metabolites, wherein the RNAs are extracted after the samples are treated. The research method helps patients suffering from the human azoospermia and the severe oligozoospermia to be rapidly detected and diagnosed, and the kit can help the patients to rapidly find pathogenesis for treatment.

The Chinese medicine preparation for treating azoospermatism features its recipe comprising wolfberry fruit, dodder seed, mulberry, Chinese yam, white peony root and other nine kinds of Chinese medicinal materials in certain weight proportion. The Chinese medicine preparation of the present invention has the functions of benefiting kidney, nourishing Yin, dispersing the stagnated liver energy, alleviating mental depression, regulating liver and kidney and producing sperm, and may be used widely in treating mení»s azoospermatism causing sterility.

The invention belongs to the technical field of traditional Chinese medicines, in particular discloses a Chinese medicinal composition for treating necrospermia, asthenospermia oligospermia, or aspermatism, which comprises the following raw material medicaments in part by weight: 10 to 20 parts of epimedium brevicornum, 10 to 20 parts of medicinal indianmulberry root, 10 to 20 parts of desertliving cistanche, 10 to 20 parts of south dodder seed, 10 to 20 parts of siberian solomonseal rhizome, 10 to 20 parts of Chinese angelica, 7 to 13 parts of common curculigo rhizome, 8 to 16 parts of Chinese thorowax root, 8 to 16 parts of large-headed atractylodes rhizome, 14 to 26 parts of astragalus, 14 to 26 parts of radix rehmanniae praeparata, 14 to 26 parts of dioscorea root, 3 to 8 parts of liquorice root, 5 to 13 parts of glossy privet fruit, 10 to 20 parts of plantain seed, 10 to 20 parts of palmleaf raspberry fruit, 20 to 40 parts of root of red-rooted salvia, 8 to 16 parts of eucommia bark, 7 to 13 parts of baicalin, 3 to 8 parts of salivia chinensis, 6 to 13 parts of Indian buead and 6 to 14 parts of oriental waterplantain rhizome. The Chinese medicinal composition has the effects of warming and invigorating kidney yang with tonifying qi and invigorating blood circulation and supplementing essence and replenishing marrow, and has the advantages of simple preparation, convenient administration, good curative effect and no toxic or side effect.

The invention discloses a separation and culture method and application of human testis mesenchymal stem cells. The separation method comprises the steps that an obstructive azoospermia patient testis specimen which is processed through tissue biopsy separation is digested with type IV collagenase, digested cell suspension is filtered to remove tissue clumps, and human testis cells are obtained; the human testis cells are marked with a CD51 antibody, separation is performed with a flow cytometer, the cells expressing the CD51 are collected, and then the human testis mesenchymal stem cells with the positive CD51 are separated. The invention further provides the human testis mesenchymal stem cells which are obtained through the separation method and have the positive CD51, the culture method of the stem cells and application of the stem cells in preparation of medicine for treating related diseases caused by low testosterone level.

The invention relates to a biomarker, and particularly relates to a serum miRNA biomarker composition and an application thereof. The serum miRNA biomarker composition comprises at least one miRNA and sequences of reverse transcription primers, forward primers and reverse primers of all miRNAs. The serum miRNA biomarker composition can be used for preparing oligozoospermia and azoospermia detection reagents. An oligozoospermia and azoospermia detection kit comprises the serum miRNA biomarker composition, Taq enzyme, dNTP, MgCl2 and a PCR buffer solution. The serum miRNA biomarker composition provided by the invention has the advantages of high sensitivity, low sample consumption, wide detection range and wide linear quantitative range.

The invention relates to a preferential sperm freezing culture device which comprises freezing slide glass, a sperm tank, a sperm swimming channel, a collecting tank and a blocking piece. The volume of the sperm tank ranges from 50 microliters to 500 microliters, the volume of the collecting tank ranges from 5 microliters to 20 microliters, the width of the sperm swimming channel ranges from 10 micrometers to 30 micrometers, and the sperm swimming channel is connected with the sperm tank and the collecting tank. The preferential sperm freezing culture device has the advantages that the preferential sperm freezing culture device is applicable to screening preservation of frozen sperm, motile sperm can be automatically separated by the design of the sperm tank, the sperm swimming channel andthe collecting tank, operation amount is decreased, mechanical damage to the sperm caused by manual picking is avoided, the proportion of usable motile sperm is increased, the freezing slide glass ofall existing sperm can be completely replaced, the preferential sperm freezing culture device can be applied to the fields of normal sperm optimization sperm periodic IVF (in-vitro fertilization), ICSI (intracytoplasmic sperm injection) or artificial insemination, sperm collection of oligospermia and asthenospermia patients, surgical sperm extraction of azoospermatism patients, sperm collection and the like, and has a wide popularization and application prospect.

The invention provides a spermatogenic pill having the functions of warming yang, tonifying kidneys and strengthening the essence. The spermatogenic pill is mainly used for treating azoospermatism. The spermatogenic pill has excellent treatment effect for patients having spermatogenic cells without vas deferens obstruction. The traditional Chinese medicine composition is prepared from the following more than twenty medicinal raw materials by weight: herba epimedii, semen cuscutae, the fruit of Chinese wolfberry, desertliving cistanche, fish glue, goat testicle, male silk moth, human placenta, semen allii tuberose, red ginseng, fleece-flower root, rehmannia glutinosa, rhizoma curculiginis, rhizoma curculiginis, fructus psoraleae, cinnamon, cooked monkshood, angelica sinensis, the root of red-rooted salvia and the like. The spermatogenic pill targets man sterility, has the functions of warming yang, tonifying kidneys and strengthening the essence and is mainly used for treating azoospermatism. The spermatogenic pill can take effect quickly for patients having spermatogenic cells without vas deferens obstruction. Natural Chinese herbal medicines does not have side effects on the body. The formula has the functions of strengthening the essence and effectively increasing the number of sperms.

The invention discloses an NANOS2 marker for predicating or diagnosing azoospermia or oligospermia. The NANOS2 marker can be DNA or mRNA of NANOS2 or protein coded by the NANOS2. The invention furtherprovides an NANOS2 polyclonal antibody, and an in vitro diagnostic reagent prepared according to the NANOS2 marker. The in vitro diagnostic reagent is used for predicating or diagnosing azoospermia or oligospermia, has the advantages of high convenience and rapidness, has high accuracy and specificity, can be applied to large-scale screening, can further serve as a clinical detection means, and particularly provides a novel approach for the diagnosis and detection of SCO azoospermia.

The invention discloses an application of a serum miRNA combination as molecular marker for assessing non-obstructive azoospermia. The serum miRNA combination includes hsa-miR-1263, hsa-miR-221-5p, hsa-miR-483-3p, hsa-miR-4275 and hsa-miR-194-3p. Through the miRNA combination, a reagent kit for assessing the non-obstructive azoospermia is prepared. The serum miRNA for assessing the non-obstructiveazoospermia is obtained, further a detection reagent kit containing primers used for quantitative RQ-PCR detection of the serum miRNA combination is obtained, detection is performed through the detection primers and the detection reagent kit, the operation is simple, the accuracy is high, and the serum miRNA combination is worth of extensive popularization.

The invention discloses a drug for treating prostate diseases, erectile dysfunction, premature ejaculation and sterility and a reparation method of the drug. The drug contains the following raw materials in parts by weight: 45-55 parts of rhizoma curculiginis, 45-55 parts of herba epimedii, 90-110 parts of actinolite, 8-12 parts of carthamus tinctorius, 18-22 parts of honeycomb, 8-12 parts of malesilk moth, 18-22 parts of wolfberry, 25-35 parts of pimoinella candolleana and 13-17 parts of cassia twig. The preparation method comprises the following steps: adding water which is 6-8 times of thetotal mass of the raw material drugs into the raw material drugs, boiling with high heat, continuing to carry out decoction for 25-35 minutes with soft fire, and extracting a decoction solution; adding water which is 5-7 times of the total mass of the medicine dreg into the medicine dreg, boiling with high heat, continuing to carry out decoction for 20-30 minutes with soft fire, combining two decoction solutions, and uniformly mixing so as to obtain the drug, or preparing medical wine by virtue of a conventional method. The drug is used for treating male diseases of prostate diseases, erectile dysfunction, premature ejaculation, sterility and the like caused due to oligospermia, low survival rate and azoospermia and has the characteristics of high effect taking speed, good treatment effect, safety and no toxic and side effects. The total effective rate of the drug is 95% proved by being clinically taken by 200 patients.

The Chinese medicine preparation for treating azoospermatism has the recipe comprising pilose antler, deer gelatin, red ginseng, astragalus root, wolfberry fruit and other 11 kinds of Chinese medicinal materials in certain weight proportion. The present invention has the functions of nourishing Yin, invigorating kidney, promoting blood circulation to disperse blood clots, complementing Yin and Yang, dredging meridian and producing sperm. The clinical application shows that the Chinese medicine preparation may be used widely in the treatment of maleí»s azoospermatism caused sterility.

The invention belongs to the technical field of medical detection, and discloses a specific marker screening method for detecting azoospermia and severe oligospermia. The method comprises the following steps: collecting a sample, treating the sample, and screening out specific markers of the severe oligospermia and azoospermia; in a testicular tissue level, screening expression of differential microRNA in testicular tissues of normal males and sterile males by using a chip method; in a seminal plasma layer, searching differential marker proteins of normal males and sterile males by using a proteomics method; in the urine level, employing metabonomics for screening the difference of metabolites in urine of normal men and infertility men; integrating data obtained from three high-flux layers, namely a testis tissue layer, a seminal plasma layer and a urine layer, and carrying out correlation analysis on the data and sperm quality, density and vitality; and optimizing and establishing a diagnosis technology. The method is long in detection time, high in efficiency and capable of explaining the cause of dyszoospermia of the patient.

The invention discloses a preparation method and application of a chemiluminiscence detection kit for quantitatively detecting TEX101 concentration, belongs to the field of in-vitro diagnostic reagents, and provides a TEX101 chemiluminiscence detection kit by researching TEX101 concentration difference results of obstructive azoospermia OA and non-obstructive azoospermia NOA. The kit can rapidly and quantitatively detect the concentration of TEX101 in seminal fluid, the sensitivity of identifying obstructive azoospermia OA and non-obstructive azoospermia NOA is 73%, the specificity is 100%, and the kit has a good prediction value for assisted reproduction results.

The invention relates to the field of animal models, in particular to a construction method and application of an azoospermia mouse model. According to the construction method of the azoospermia mouse model, a Pus7 gene on a mouse genome is knocked out. The important role of the Pus7 (Pseudouridine synthase 7) gene in the spermatogenesis process of mice is disclosed for the first time, male mice knocked out by the Pus7 have testicular dysplasia and azoospermia, and a mechanism of regulating testicular development by the Pus7 is expected to provide a new thought for research of male infertility mechanisms.

The invention provides a decoction for promoting fertility and nourishing kidney. The decoction has functions of nourishing kidney and yin and warmly invigorating kidney yang and can be mainly used for treating male infertility caused due to deficiency of kidney yin and yang, gonacratia or spermatorrhea, rare semen, oligospermia and poor sperm motility. Traditional Chinese medicine composition of the decoction is prepared from the following raw materials according to weight: prepared rhizome rehmannia, pulp of dogwood fruit, semen cuscutae, radix achyranthis bidentatae, tortoise plastron and antler pieces. The decoction is excellent effect of treating azoospermatism, oligospermia and infertility with normal sperms, is excellent in effect of treating male infertility and can become effective quickly. The decoction is prepared from natural traditional Chinese medicine components, so that the decoction is free of side effect; the formula of the decoction has the functions of strengthening sperms and effectively increasing the number of the sperms.

The invention discloses application of maca and maca polysaccharide in treating testis spermatogenesis disorders, and particularly relates to the application of the maca and the maca polysaccharide intreating the spermatogenesis disorders including oligospermia, asthenozoospermia and azoospermia, especially azoospermia caused by medicine. According to the application, an azoospermia model of a busulfan-induced mouse is used for verifying the improvement effect of the maca and the maca polysaccharide on the busulfan-induced azoospermia mouse.

The invention discloses pathogenic mutation of genetic gametogenesis disorder and a detection reagent thereof. The invention relates to a mutant Pof1b gene, the mutant Pof1b gene is located in a chromosome X and is deletion mutation c.312_316delp.S104fs, the gene number of the wild type Pof1b gene in an Ensemble database is ENSG00000124429, the physical position of the deletion mutation Pof1b geneis a third exon, five bases TACAT are deleted at chrX-84622737-84622742, and following sequences cause frameshift mutation. The mutant Pof1b gene or the mutant POF1B protein is used as a detection target to be applied to preparation of an auxiliary diagnosis reagent or detection equipment for male non-obstructive azoospermia or female hereditary primary ovarian insufficiency.

The invention provides a drug for preventing and curing azoospermia and oligozoospermia of cocks. The drug is composed of the following six traditional Chinese medicines: radix acanthopanacis senticosi, rhizoma curculiginis, herba epimedii, semen cuscutae, fructus cnidii and actinolitum. The drug has the advantages of reasonable prescription, simple processing, feed added administration and significant effect in preventing and curing of azoospermia and oligozoospermia of cocks.

The invention relates to an application of a C12orf40 gene. In clinical scientific research and practice, the inventor screens and identifies a disease-causing gene C12orf40 cleavage site variation (NM001031748.4, c.1286 + 1Ggt; a), no haploid sperms are found in testis biopsy tissues of a patient, and non-obstructive azoospermia is shown. The gene is specifically expressed in testis tissues, is not expressed in other tissues, can be used as a biomarker of the non-obstructive azoospermia, and is beneficial to further research on a mechanism of the non-obstructive azoospermia and exploration on treatment of the non-obstructive azoospermia.