23 results about "Motile sperms" patented technology

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.

Systems for sorting sperm include a housing and a microfluidic system supported by the housing. The systems also include two or more inlets providing access to the microfluidic system to deliver sperm or semen and fluid to the microfluidic system, as well as an outlet for harvesting sorted sperm. The systems include a flow channel that provides a flow path for sperm from an inlet to an outlet while sperm travels against a fluid flow towards an outlet for harvesting. In the systems, fluid delivered to the microfluidic system via an inlet flows from the inlet towards one or more outlets, and the flow channel extending along the length of the microfluidic system provides a flow path for motile sperm to travel against the fluid flow towards a collection outlet for harvesting. In some embodiments of a system, the microfluidic system also includes a microporous filter arranged in the flow path between the inlet and the collection outlet to cause motile sperm traveling against the fluid flow to pass through the filter to reach the collection outlet for harvesting.

The present invention relates to the field of genetic breeding technology, and specifically discloses a SNP molecular marker, primer, and application related to linear motility of sperm after cryopreservation and thawing of chicken semen. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO.1, and the 53rd position in the SNP molecular marker is a SNP site, which has a G / A polymorphism. The SNP molecular marker provided by the present invention has a SNP site at position 53, which has a G / A polymorphism. The proportion of linearly motile sperm in semen of individuals with a GA genotype after cryopreservation and thawing of semen is higher than the proportion of linearly motile sperm in semen of individuals with an AA genotype after cryopreservation and thawing of semen.

The application discloses a high-precision sperm morphology screening system and a screening method, and relates to the technical field of sperm cell recognition. Raw semen is obtained and filtered to obtain initial semen; the initial semen is spread into a preset single-layer sperm liquid strip to obtain initial single-layer motile sperm; a first magnification operation is performed on the initial single-layer motile sperm to obtain a first initial single-layer motile sperm image; a second magnification operation is performed on the first initial single-layer motile sperm image to obtain a second initial single-layer motile sperm image; and the second initial single-layer motile sperm image is substituted into a high-precision sperm morphology selection model to obtain target high-quality sperm. Through the preliminary filtration of the raw semen and the secondary screening of the preset single-layer sperm strip spreading method, and by adopting the progressive magnification strategy of the first magnification operation and the second magnification operation, the high-precision sperm morphology screening can be performed when high-quality sperm is selected by using the existing IMSI technology, and the screening efficiency of the high-precision sperm is improved.

The present application discloses an artificial propagation-hybridization combined breeding method for meat goats, which relates to the technical field of animal breeding. The specific steps include: selecting a goat breed with good meat performance as the maternal parent and selecting a goat breed with a large body as the paternal parent; culturing the maternal oocytes in vitro to mature oocytes containing the first polar body, using a temperature gradient screening device to screen out highly motile sperm, fertilizing the mature oocytes with the highly motile sperm in vitro, culturing them to a hybrid blastocyst morphology, transplanting them into the maternal body to develop until natural delivery, and obtaining a hybrid F1 generation; backcrossing the F1 generation with the maternal parent, and backcrossing the backcross offspring with the maternal parent multiple times until the breeding goal is met, and then cross-crossing and fixing them to obtain a new breed / line with excellent traits; improving the sperm screening efficiency by utilizing the screening device, ensuring the quality of the screened sperm, and thus significantly improving the fertilization efficiency and embryo survival rate.

To provide a motile sperm sorting device that can easily and efficiently sort motile sperm and suppress DNA damage to motile sperm, and to provide a method for sorting motile sperm.SOLUTION: The present invention relates to a motile sperm sorting device that includes a supply tank 4 that contains a sperm-containing liquid that contains sperm, a collection tank 5 that contains a liquid medium, and multiple flow paths 6 that connect the supply tank 4 and the collection tank 5 to each other. By generating a flow of medium from the collection tank 5 to the supply tank 4 in the multiple flow paths 6, motile sperm contained in the sperm are made to migrate upstream from the supply tank 4 to the collection tank 5 through the multiple flow paths 6, and the motile sperm are sorted.SELECTED DRAWING: Figure 2

Disclosed is a device for collecting motile sperm, the device comprising a post having a top, a bottom, and a wall, the wall comprising a mesh layer and a membrane layer, an inlet disposed at the top of the post, and a nose disposed at the bottom of the post.

A system and method for improving sperm selection during in vitro fertilization (IVF) procedures includes a sperm containment apparatus equipped with a capture system designed to isolate motile sperm as they respond to the presence of a retrieval instrument within the sperm solution. As more motile and potentially healthier sperm move away from the retrieval instrument, they are selectively corralled within certain regions of the apparatus. The capture mechanism may include various structures, such as a barrier member including one or more passageways which direct sperm movement. By isolating sperm based on their motility, this invention enhances the selection process, improving the chances of successful fertilization with higher-quality sperm. This system provides a more efficient and targeted approach to sperm retrieval compared to conventional methods.

[Problem] To improve a sperm yield. [Solution] A sperm sorting device 1 comprises: a bottom wall 11; a side wall 12; and an internal space 13 defined by the bottom wall 11 and the side wall 12. The internal space 13 has: an input part 31; a collection part 32; and a communication part 33 for connecting the input part 31 and the recovery part 32. The communication part 33 includes: a bottom surface 43; and an inner wall 44 extending upward from the bottom surface 43 and across the input part 31 and the recovery part 32. Motile sperm 200 introduced into the input part 31 move to the collection part 32 along the bottom surface 43 of the communication part 33 and a surface of the inner wall 44. Even motile sperm 200 with a low acceleration against gravity can move from the input part 31 to the collection part 32 through the communication part 33.

Kits and methods for semen collection and filtration are provided. The kits and methods provide for simple and reliable collection of semen samples away from a clinical setting. The kits and methods also provide for more rapid processing of semen samples, selecting motile sperm through passive filtration approaches, which can be beneficial even when used in a clinical setting. In a broad sense, the kits include a semen collection device such as a condom, a base having a reservoir, and a filtration cap allowing for passive filtration of motile sperm. Methods of using the kits are also provided.

A device for filtering out non-functional sperm and separating motile sperm may include a main channel having a flow direction from a first end toward a second end of the main channel. The device may include a buffer inlet in fluid communication with the first end of the main channel. The apparatus may include a collection chamber in fluid communication with the second end of the main channel. The device may include a secondary channel in fluid communication with the collection chamber on a downstream side. The apparatus may include a waste chamber in fluid communication with the secondary channel on a downstream side.

The application relates to the field of animal breeding technology, in particular to a method and system for detecting total live sperm count of a cow and application. The method comprises the following steps: based on the genome version ARS-UCD1.2, the methylation level of a region such as chr17:62205563-63205744 of a to-be-detected cow is detected; the detection result is compared with a reference standard, and the total live sperm count of the to-be-detected cow is obtained; the reference standard is obtained based on GWAS correlation data of the total live sperm count and the methylation level of a reference cow group in the region of chr17:62205563-63205744. A region significantly related to the total live sperm count of the cow is obtained through research, early identification of the total live sperm count of the cow can be realized by detecting the methylation level of the region, and this has important significance in the field of animal phenotype identification.

A sperm sorting device is suitable for sorting a plurality of motile sperm from a semen sample, and includes a base and a first sorter positioned on the base. The base includes a receiving space defined by a bottom wall and a surrounding wall. The first sorter includes a first kit and a first permeable membrane. The first kit has a first sleeve for being placed into the receiving space. The first sleeve has a first inner sleeve surface and a first outer sleeve surface. The first permeable membrane is fixed to the first sleeve. The first permeable membrane and the inner wall surface of the bottom wall and the surrounding wall define a first sample receiving chamber for receiving the semen sample. In this way, the motile sperm can naturally swim upward and pass through the first permeable membrane, and the quality of sperm sorting can be improved.

This invention discloses a barrel-shaped sperm selection device for selecting highly motile sperm from semen samples. The device includes a container with a porous membrane inside, which divides the interior of the container into an initial chamber and a filtration chamber. This allows the semen sample to move through the porous membrane to the filtration chamber, achieving the selection purpose. The advantages of this invention are: the overall barrel shape of the device, with the porous membrane arranged on a hub along the axial direction of the cylindrical container to form inner and outer cavities, simplifies the structure and optimizes the principle, greatly reducing the requirements for molds and processes. The barrel-shaped structure with inner and outer cavities prevents semen overflow even when the semen sample volume is too large; and prevents reduced selection rates due to air bubbles even when the semen sample volume is too small.

Systems and methods for male fertility analysis may include: loading an aliquot of a male fertility sample into a chamber of an analysis system via an inlet, the chamber having an opening covered over by a porous membrane and a concave bottom opposite the opening; introducing media so that the membrane acts as a migration path for motile sperm; removing the media and a motile portion of the aliquot from the system into a container; sealing the container; and transporting the sealed container and a remainder of the male fertility sample to a testing facility. The testing facility may: determine a first concentration of the portion of the sample from the first container; determine a second concentration of the remainder; and estimate a percentage of total motile sperm for the sample based on the first concentration, the second concentration, and a predetermined relationship between portions and remainders of samples.

The present invention relates to a chip for realizing high-motility sperm screening based on microfluidic technology, comprising a substrate and a slide, wherein the substrate is used to support the slide; a loading pool is provided on the slide, the loading pool comprises a sample inlet, and a grading pool and a collecting pool are sequentially provided on the periphery of the loading pool; the loading pool, the grading pool and the collecting pool are sequentially connected; a sampling port is also provided on the slide, and the sampling port is connected to the collecting pool. The present invention solves the potential adverse effects caused by various operations such as sperm centrifugation and washing in vitro in the existing assisted reproductive technology process. It utilizes sperm chemotaxis and swimming characteristics along the interface to effectively screen high-motility sperm, obtain high-motility sperm required for assisted reproductive technology, and greatly improve the success rate of in vitro fertilization. It has a wide range of applications, and the device can be used in human reproduction and animal breeding.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.

Systems and methods for male fertility analysis may include: loading an aliquot of a male fertility sample into a chamber of an analysis system via an inlet, the chamber having an opening covered over by a porous membrane and a concave bottom opposite the opening; introducing media so that the membrane acts as a migration path for motile sperm; removing the media and a motile portion of the aliquot from the system into a container; sealing the container; and transporting the sealed container and a remainder of the male fertility sample to a testing facility. The testing facility may: determine a first concentration of the portion of the sample from the first container; determine a second concentration of the remainder; and estimate a percentage of total motile sperm for the sample based on the first concentration, the second concentration, and a predetermined relationship between portions and remainders of samples.

The invention discloses an in-vitro fertilization failure risk prediction model and a construction method and a prediction method thereof, based on data of an ART treatment cycle, multi-dimensional indexes such as woman age, BMI, male seminal fluid parameters and infertility years are incorporated, logistic regression, a random forest and an XGBoost model are constructed, and performance is evaluated by adopting a nested cross validation framework (including an SMOTE technology). Results show that the logistic regression model is optimal in performance and is remarkably superior to a random forest model and an XGBoost model, key predictive factors include male age, female BMI, the total number of forward motional sperms and a sperm DNA fragmentation index, the total number of forward motional sperms and the male age are protection factors, the BMI and the sperm DNA fragmentation index are risk factors, and the risk factors are the risk factors. The C-index of the model column diagram is up to 0.722 through internal verification, and the model column diagram has the clinical distinguishing capability of medium or above. The logistic regression model is verified to have high efficiency and stability in IVF fertilization failure prediction, and can provide decision support for clinical early intervention.

A method for enhancing artificial insemination (AI) in mammals, particularly bovine livestock, uses a specialized aqueous mastic solution to substantially increase conception success rates from conventional 60-65% to about 95%. The solution comprises ionic minerals including magnesium chloride, calcium chloride, sodium, potassium, and an emulsion of milled mastic gum (Pistacia lentiscus, preferably Chios Mastiha, particulates 15-40 μm) dissolved in water sourced from inland saltwater bodies such as the Great Salt Lake, delivering approximately 72 ionic and trace minerals. The process involves: preparing the uterus by depositing the solution via syringe to maintain pH at 7.35-7.45 and eliminate bacteria; washing donor semen in the solution to isolate healthy, motile sperm and remove debris / reactive elements; and depositing the washed semen directly into the uterus during ovulation, confirmed via luteinizing hormone detection, ultrasound, or chorionic gonadotropin induction.

Systems and methods for male fertility analysis may include: loading an aliquot of a male fertility sample into a chamber of an analysis system via an inlet, the chamber having an opening covered over by a porous membrane and a concave bottom opposite the opening; introducing media so that the membrane acts as a migration path for motile sperm; removing the media and a motile portion of the aliquot from the system into a container; sealing the container; and transporting the sealed container and a remainder of the male fertility sample to a testing facility. The testing facility may: determine a first concentration of the portion of the sample from the first container; determine a second concentration of the remainder; and estimate a percentage of total motile sperm for the sample based on the first concentration, the second concentration, and a predetermined relationship between portions and remainders of samples.

The invention relates to a microfluidic device, method, and use for the continuous, label-free isolation of motile, morphologically normal spermatozoa from semen samples This invention offers a gentle, reliable, and efficient platform for sperm selection, serving as an alternative to centrifugation-based techniques in ART laboratories. By maintaining the structural and genetic integrity of sperm, it improves fertilization success, embryo quality, and pregnancy outcomes. The platform also reduces variability between technicians and limits exposure to reactive oxygen species (ROS), ensuring consistent clinical performance. Beyond its application in fertility treatments, the system can be used as a diagnostic tool to assess sperm motility disorders, susceptibility to oxidative stress, and DNA fragmentation dynamics under different environmental conditions or drug exposures.

The invention discloses a high-precision sperm morphology screening system and screening method, and relates to the technical field of sperm cell recognition. Obtaining original semen and filtering to obtain initial semen; paving the initial semen into a preset single-layer sperm liquid strip to obtain initial single-layer motile sperms; performing a first amplification operation on the initial single-layer moving sperm to obtain a first initial single-layer moving sperm image; performing second amplification operation on the first initial single-layer moving sperm image to obtain a second initial single-layer moving sperm image; and substituting the second initial single-layer moving sperm image into a high-precision sperm morphology selection model to obtain a target high-quality sperm. According to the sperm morphological screening method, primary filtration of original sperm, secondary screening of a preset single-layer sperm strip laying method and a progressive amplification strategy of the first amplification operation and the second amplification operation are adopted, so that the problem that high-precision sperm morphological screening can be performed when high-quality sperms are selected by the existing IMSI (International Mobile Subscriber Identity) technology is solved, and the screening efficiency of the high-precision sperms is improved.