38 results about "Sperm morphology" patented technology

The invention belongs to the technical field of image recognition, and particularly relates to a medical sperm image recognition system based on deep learning. The system comprises an input module, apositioning module and a classification module, wherein the input module is used for collecting sperm pictures after graying processing of a detector; the positioning module is used for positioning asperm head on the sperm picture by utilizing a YOLO v3 model in a deep learning and image recognition method according to the sperm picture acquired in the input module; the classification module adopts the constructed VGG-sense block classification model to carry out positive anomaly judgment on the sperm heads positioned in the positioning module, and outputs normal sperms and abnormal sperms. The system is short in sperm picture detection time, greatly reduces the workload of doctors, is high in accuracy, reduces errors caused by subjectivity, can assist and partially replace the doctors toperform sperm morphology evaluation, and has a good application prospect.

The invention relates to a noninvasive sperm form and ultrastructure analysis method. The method comprises the following steps: analyzing by using a dispersion type confocal Raman spectrometer, selecting an excitation Raman spectrum, placing sperm cells under a reflected light microscopy, and acquiring the chemical group spectrum of the sperm cells through a certain excitation power and a certain exposure time; respectively dividing the acrosome, the neck and the tail of a sperm into scanning areas, scanning three times to obtain a Raman data spectrum peak, and analyzing the waveform change and the displacement of the spectrum peak; and retrieving the acquired sperm form Raman spectrums in an RRUFF Raman database to obtain the Raman spectra of all areas of the sperm form. The laser Raman spectrum adopted in the invention is a noninvasive technology, so the characteristics of the ultrastructures of different individuals and the scattering substances of different components of each sperm can be reflected without any dye or fixation, and a new assessment method is provided for the sperm morphology analysis.

The invention discloses a sperm morphology detection method and device based on an image technology, and relates to the computer vision field. A specific embodiment of the method comprises the following steps: identifying any one independent sperm in a to-be-detected sperm image; obtaining contours of key parts of the independent sperms; determining the central coordinates of the key parts of theindependent sperms according to the contours of the key parts of the independent sperms; based on the to-be-detected semen image and the central coordinates of the key parts of the independent sperms,extracting the contours of the independent sperms; and performing morphological detection on the independent sperms according to the contours of the independent sperms. According to the embodiment, the sperm detection efficiency is improved.

The invention belongs to the field of cytobiology research, and specifically belongs to the field of sperm morphology positioning research. A grid positioning sheet disclosed in the invention comprises a glass substrate, and a polished glass sheet; the glass substrate and the polished glass sheet are glued together; the polished glass sheet is provided with a square grid zone via drawing; the square grid zone comprises 25 1mmX1mm big grids arranged with intervals of 0.1mm; the top left of each big grid is marked with a capital letter selected from A to Y; each big grid comprises 100 0.1mmX0.1mm square small grids; along the side frame of each big grid from the top to the bottom and from the left to the right, the small grids are marked with numbers from 0 to 9; the numbers and grid lines on the grid positioning sheet are all displayed via highlight black lines. The sperm grid positioning sheet is suitable for observing of sperm morphology under a high-power microscope; accurate positioning of the observed sperms can be realized via the big and the small grids which are marked with English letters and Arabic numerals; and it is extremely convenient to find target sperms when repeated checking on specific sperms is necessary.

The invention discloses a sperm morphology rapid dyeing reagent and a dyeing method thereof. The sperm morphology rapid dyeing reagent is prepared from a solution A, a solution B and a solution C; thesolution A is tissue fixative containing fast green; the solution B is a preservation solution containing eosin; and the solution C is phosphate buffer containing alkaline azure A and methylthioninechloride. The dyeing method comprises the steps that fresh seminal fluid is collected and liquidized in a water-bath pot with the temperature being 20-37 DEG C, sperms are optimized for 8-12 minutes by adopting a swim-up method, and the fresh sperms with high activity are obtained; sperm suspension and a smear are prepared, and the smear is dried under a sterile environment; the smear is dipped into the solution A and reacts for 15 seconds at room temperature so as to remove redundant fixative; the smear is dipped into the solution B and reacts for 10 seconds at room temperature so as to remove the redundant dyeing agent; the smear is dipped into the solution C and reacts for 10 seconds at room temperature so as to remove the redundant reagent; the redundant dyeing agent is removed; moisture is removed, and after the smear is completely dried, dyeing is completed. The sperm morphology rapid dyeing reagent well solves the defects that sperm dyeing is complex to operate, and the cost ishigh in the prior art.

The invention provides a multifunctional sperm quality analysis system. The multifunctional sperm quality analysis system comprises sperm routine computer-aided sperm analysis (CASA) and image analysis, the CASA is used for analyzing a sample video recorded through a CCD camera or video inputted into the local of an analysis host to calculate the quantity, the concentration, the vitality and the motion trail of sperms of the sample; the image analysis is used for analyzing sperm functions and comprises sperm DNA fragment detection, sperm nucleoprotein detection and sperm morphological detection, and pictures taken through the CCD camera or pictures inputted to the local of the analysis host are analyzed. The problems that existing seminal fluid analysis operation is tedious, human factorsare uncontrollable, and the inspection cost is high are solved.

The invention relates to the technical field of medical apparatus and instruments, in particular to an automatic sperm morphological detection slide making device and method. The automatic sperm morphology detection slide making device comprises a control system, a sample loading device, a dyeing device, a flushing device and a blow-drying device. The sample loading device consists of a sample loading groove; the dyeing device is composed of dyeing liquid pipes (including a first dyeing liquid pipe and a second dyeing liquid pipe), a liquid inlet and a liquid outlet, the flushing device is composed of a spray head and a flushing pipeline, and the blow-drying device is composed of an air dryer (including a front baffle, a rear baffle, fan blades, a motor and an air bellow). The invention further comprises a button and a power supply. According to the invention, automation of sperm morphological detection slide making is realized, the operation is simple, the carrying is convenient, flaking precision can be realized while flaking is rapid, and the practicability is very high.

The invention relates to a method for inducing skin stem cells to differentiate into sperms. The method comprises the following steps of firstly, co-culturing the skin stem cells of a mouse and fibroblast treated by mitomycin C; inducing to differentiate into PGCs (primordial germ cells); then, reversely injecting PGC cell mixing liquid into a testis seminiferous tubule through an efferent ductule of a recipient mouse; after developing for one month in the testis of the recipient mouse, fetching out the testis of the recipient mouse; after HE staining analysis, finding the recovery of spermatogenesis in the seminiferous tubule, emerging of cells with typical sperm morphology, and expression of GFP and MVH of spermatogenic cells in the seminiferous tubule with recovery of spermatogenesis. The method has the advantages that the injury to a body is little, the limitation of ethical issue is avoided, the skin stem cells are effectively induced to be differentiated into sperm cells, the differentiation efficiency is high, the sperm cells obtained by differentiation have typical sperm morphology, and the specific gene of the sperm cell is expressed.

The invention provides a compound traditional Chinese medicine for treating masculine sterility, which comprises the following components in parts by mass: 8-15 parts of polyporus umbellatus, 6-12 parts of tuckahoe, 6-12 parts of oriental waterplantain rhizome, 6-12 parts of talcum, 10-30 parts of donkey-hide gelatin, 10-30 parts of rehmannia glutinosa, 8-15 parts of China dodder, 8-15 parts of raspberry and 4-6 parts of leech. The compound traditional Chinese medicine disclosed by the invention is mainly used for treating sterility due to male dysspermatism; after being verified by a large amount of clinical tests, the compound traditional Chinese medicine can be used for quickly recovering various objective indicators of dysspermatism patients, such as sperm count, sperm morphology, sperm pH value, sperm viscosity, sperm liquefying time, sperm motility, sperm viability rate and the like to be normal within one to three weeks, thereby the successful conception is achieved. Meanwhile, the compound traditional Chinese medicine has definite curative effect for sterility caused by sperm immune abnormality. By administrating the compound traditional Chinese medicine, the quality of semen can be improved as soon as possible, thereby the semen is ensured to be normal and impregnated.

A semi-automatic sperm staining instrument, comprising a rectangular base, several staining solution containers arranged side by side along the long sides at intervals on the base, and a padding device arranged above the base, the padding device includes a long side and a base A pressing plate with a horizontal short side, several sperm-coated glass sheets arranged on the pressing plate, sliding connection devices hinged at both ends of the lower long side of the pressing plate, so that the pressing plate can be turned up and down and reciprocally slide along the long side of the base. The pressure dyeing method and the use of special instruments to control the time also solve the problem of easy mixing of dyes. At the same time, there are washing and air-drying devices, and the standardization of sperm staining can be achieved at a fixed room temperature. It solved the problem of standardization of sperm morphology staining and laid the foundation for the standardization of sperm morphology diagnosis.

A sperm morphological fast stain consists of solution A containing Nile blue fixation liquid of tissues for staining sperm apical body region. A method for fast staining includes preparing sperm suspension film preparation, drying by air, using said solution A to cover film preparation till sperm apical body region be stained.

A method of automated measurement of motility and morphology parameters of the same single motile sperm. Automated motility and morphology measurements of the same single sperm are performed under different microscope magnifications. The same single motile sperm is automatically positioned and kept inside microscope field of view and in focus after magnification switch. A method of automated non-invasive measurement of sperm morphology parameters under high magnification of imaging. Sperm morphology parameters including subcellular structures are automatically measured without invasive samplestaining. A method of automatically selecting sperms with normal motility and morphology and DNA integrity for infertility treatment.