117 results about "Male mice" patented technology

Mice are provided that comprise a reduction or deletion of ADAM6 activity from an endogenous ADAM6 locus, or that lack an endogenous locus encoding a mouse ADAM6 protein, wherein the mice comprise a sequence encoding an ADAM6 or ortholog or homolog or fragment thereof that is functional in a male mouse. In one embodiment, the sequence is an ectopic ADAM6 sequence or a sequence that confers upon a male mouse the ability to generate offspring by mating. Mice and cells with genetically modified immunoglobulin heavy chain loci that comprise an ectopic nucleotide sequence encoding a mouse ADAM6 or functional fragment or homolog or ortholog thereof are also provided.

The invention provides a method for establishing an SD mouse fat and diabetes research model through diet and STZ induction. Through high-fat feed and STZ induction, the attacking characteristics of fat and diabetes of humans are induced, and an animal model for establishing pathogenesis of fat and type-2-diabetes caused by insulin resistance and partial defects of pancreatic beta cells is provided. Mice are divided into a normal control group, a fat group and a diabetes group, and each group has six SD male mice. The high-fat feed is adopted for feeding mice for eight weeks, a pure-fat insulin resistance model is induced, after the mice are fed with the high-fat feed for eight weeks, injection of STZ small doze in batch is conducted, and establishment of the diabetes animal model is induced. The method can better simulate the attacking characteristics of human type-2-diabetes caused by improper diet and enjoinment factors currently, and compared with other modeling, the method has better scientific significance of clinical and fundamental research combination.

The invention discloses an imaging model in small animal living bodies with echinococcus granulosus and a construction method thereof. The construction method comprises the steps of: feeding CF-1 male mice; carrying out in vitro culture and drug treatment on protoscolex; carrying out in vitro fluorescence imaging and fluorescence gradient analysis; pretreating mice with depilatory paste for experimental animals, injecting the abdominal cavity of each mouse with chloral hydrate anesthetic, implanting three groups of stained scoleces under Glisson capsules by injection, dividing the mice into three groups according to different groups of injected protoscolex; in a control group, injecting the livers of mice with protoscolex on which drug treatment is not carried out, collecting images every 12 hours after injection, carrying out fluorescence intensity analysis on the ROIs region, putting into a polyethylene cage after imaging, and carrying out in vitro fluorescence gradient detection in the positive control group. The construction method constructs a mouse model with echinococcus granulosus which can express luciferase, provides a platform for drug sensitive tests in living bodies with echinococcosis, and can monitor the growth and transfer of hydatid in the living model dynamically in a long term.

The invention discloses a research method for a protective mechanism of learning and memorizing functions. The research method comprises the steps that ICR male mice are divided into groups randomly to detect the effects of learning and memorizing of the mice in different stages and different time in the classic space recognition memory experiment, and the autophagy expression of synaptosome of the mice in the cerebral cortex is detected; then gamma-aminobutyric acidreceptor antagonist dicentrine and autophagy inhibitor 6-amino-3-methyl purine are used for separating cultured ICR mouse original generation nerve and intracerebroventricular injection of the ICR mice respectively, the learning and memorizing situation of the mice is detected by using Morris water maze and conditioned fear experiment, the expression of autophagy protein and PSD protein of the cerebral cortex of the mice and the change of synaptosome morphology are detected through immunohistochemistry, and then a regulatory mechanism between NMDA receptor subtypes and the autophagy process is observed.

The invention provides an establishment method of a primary liver cancer mouse model and relates to an establishment method of a liver cancer mouse model. The invention solves the problems of an existing establishment method of the primary liver cancer mouse model that the establishment time is long, the feeding cost is high, and the death easily occurs in feeding. The establishment method comprises the following steps of: 1, selecting a 4-week-old ICR (Institute for Cancer Research) male mouse, and observing and feeding the mouse for 2 weeks; 2, after the male mouse is fed for 2 weeks, determining the body weight of each mouse and the daily water intake; 3, disinfecting the abdomen of the mouse by using alcohol cotton with a volume concentration of 75 percent, injecting DEN (Diethylnitrosamine) aqueous solution into an abdominal cavity, enabling the mouse to drink water containing DEN with a volume concentration of 0.15 percent after 12 hours and feeding for 4 weeks; 4, after 4 weeks, injecting the DEN aqueous solution into the abdominal cavity again, then feeding and drinking the water containing the DEN freely during the feeding period; 5, feeding to 10 weeks as of the day of injecting the DEN aqueous solution for the first time to obtain the primary liver cancer mouse model. The establishment method is also used for establishing an in-situ liver cancer animal model.

The invention discloses a modeling method of a depression animal model of a pubescent mouse. According to the current situation of stay-at-home children in today's society and the pathogenesis mechanism of depression, it is verified through a large number of experiments that a weanling male mouse of a C57BL/6J strain is raised through a single cage, and the depression animal model of the pubescentmouse is established on the periphery. It is shown through experimental results that the mouse of a model group produces depression-like phenotypes, including animal behaviors, corticosterone and sleeping situation, so that the modeling of the depression animal model of the pubescent mouse succeeds. The model is simple in operation, the current situations of the stay-at-home children can be verywell simulated, and the blank of depression animal models at the key pubescent development stage is also filled. Through the model, the modeling method can be very well used for screening anti-pubescent depression drugs and has the important application value in pathogenesis mechanism of pubescent depression in future.

A process for preparing the transgenic mouse able to specifically express Cre recombinant enzyme in cental nerve system includes such steps as using the terminals' regulatory sequence of 1.8 kb GFAP gene to configure the transgenic carrier pGFAP-Cre-hGH containing two beta-glubolin insulators, GFAP5' terminal regulatory region, Cre recombinant enzyme gene and human growth hormone gene, injecting the 7.6 kbtransgenic fragment beta globin insulator-pGFAP-Cre-hGH into mouse's fertilizer eggs, transplating them in the deference duct of male mouse, and developing to obtain the target mouses.

The invention provides a preparation method of a collagen-induced arthritis animal model. The method includes the steps that 6-8 week old male DBA/1 mice are selected; the mice selected in step (1) are placed and raised in a clean animal room where the temperature is 20-24 DEG C, the humidity is 50%-60%, and light and dark are alternating every 12 hours, the mice are free to take food and drink water, and after three days of acclimatization the mice are used for preparing the model; a chicken type-II collagen is used for immunizing the DBA/1 mice to induce the CIA model, and the mice are immunized by intradermal injection into the tail bases of the mice; three immunizations are conducted. The method is of great significance for the pathological mechanism study and drug development of arthritis. Meanwhile, an evaluation method of the collagen-induced arthritis animal mode, through arthritis clinical index grading, ankle joint pathology examination, and determination of serum anti-CII-IgG antibody level and other indicators, is used for verifying whether the model is successful and comprehensively evaluating the collagen-induced arthritis animal model.

The invention discloses application of Jilin ginseng oligopeptide to preparation of food or health food capable of improving and strengthening sexual functions. Jilin ginseng oligopeptide is a mixture of micromolecular biologically-active peptides extracted from Jilin ginseng by using biological enzymolysis technology. The Jilin ginseng oligopeptide is low in viscosity, high in bioavailability and fast in absorption, and the Jilin ginseng oligopeptide can be directly absorbed by the human body without digestion. Male mice are administered with an aqueous solution of the Jilin ginseng oligopeptide by intragastric injection for 30 days, and results show that the Jilin ginseng oligopeptide can increase the indexes of the accessory sexual organs of the male mice, raise the concentrations of NO and testosterone in the serum of the male mice, shorten the period of mating latency of the mice and increase mating frequency. According to the invention, the Jilin ginseng oligopeptide has obvious effect on improving and strengthening sexual functions, can be used for preparation of food or health food capable of improving and strengthening sexual functions and provides a novel approach to improvement and strengthening of sexual functions through dieting.

The invention provides a Ddx5 gene deleted spermatogenesis disorder mouse model and a construction method thereof, and the inventor finds that a relationship exists between the Ddx5 gene and spermatogenic disorder or fertility dysfunction in research, the testicle volume of a male mouse with Ddx5 gene deletion is obviously reduced compared with that of a wild mouse. Moreover, the testicle of the mouse is free of germ cells, the epididymis is free of sperms, and any offspring cannot be obtained after the mouse is mated with a female mouse. Therefore, the Ddx5 gene deleted male mouse has the advantages of stability and thorough spermatogenic function inhibition, and is a good research model for male fertility disorders.

The invention discloses application of donkey-hide gelatin to preparing drugs or health-care products for treating premature ovarian failure and belongs to the field of new drug application of donkey-hide gelatin. On the basis that a premature ovarian failure animal model is established through cyclophosphamide, influences of donkey-hide gelatin on the weight, the weight increase, the food intake amount, the estrous cycle, the ovary organ coefficient, the sex hormone level, histopathology and follicle counting and the like of premature ovarian failure animals are observed, according to the experiment result, it is found that donkey-hide gelatin has a certain effect on weight restoration of female mice after modeling of cyclophosphamide and has the tendency to increase the ovary and uterus weight and the organ coefficient of male mice, donkey-hide gelatin has a certain forward regulating effect on follicular development, it is shown that donkey-hide gelatin has the effects of increasing the ovary coefficient and enhancing the functions of the ovary, premature ovarian failure can be inhibited, and therefore donkey-hide gelatin can be applied to preparing drugs or health-care products for treating premature ovarian failure.

The invention belongs to the technical field of medicine, and specifically discloses a preparation method of a compound preparation made of angelica keiskei, dendrobium officinale and Chinese wolfberry fruits, and an application of the preparation to a medicine for preventing or treating sexual dysfunction. The compound preparation is prepared by mixing angelica keiskei extractum, dendrobium officinale extractum and Chinese wolfberry fruit extractum. Experiments show that the preparation can increase the number of bestriding in sexual behavior of castrated male mice, increase the number of sperm in sexual dysfunction, and improve the contents of serum testosterone, lutropin, follicle-stimulating hormone and serotonin in individuals with sexual dysfunction. In addition, after using the compound preparation of the present invention, the tissue structure of mouse testesis is relatively complete and distinct in gradation, and more sperm cells exist. The preparation for improving sexual dysfunction of the invention has clear effective components, determined curative effect, no toxic and side effect, easy-to-implement extraction method and broad application prospect.

The invention relates to a novel use of aromatase inhibitors, in particular to application of the aromatase inhibitors in terms of preparation of medicines for treating male bladder outlet obstruction and lower urethral syndromes caused by the same. The medicines can rapidly and effectively treat bladder outlet obstruction of male mice. The novel use can be clinically applied better, provides a fundamental basis for clinical treatment, and is a novel method for treating the bladder outlet obstruction.

The invention provides application of a pulse and blood improving preparation and leech in preparing a medicament for treating sexual dysfunction. Particularly, according to the pulse and blood improving preparation and leech, the incubation period of a male mouse in capturing a female mouse is shortened, the number of capture times is increased, the weight of prostate and semen sac of the mouse can be increased, and the index of accessory sex organ of the mouse is increased. The pulse and blood improving preparation and leech have the effect of promoting sexual function, and have certain curative effect in treating sexual dysfunction.

The invention discloses a construction method and an application of a PHF6 <delta> and JAK3<M511I> double-mutation acute T lymphocytic leukemia mouse model. The preparation method comprises the following steps: utilizing a Vav1-Cre mouse to mate with a Phf6<fl/fl> mouse to obtain Vav1-Cre; a Phf6<fl/Y> (Phf6<-/Y>) male mouse; mating the Mx1-Cre mouse and the Phf6<fl/fl> to obtain Mx1-Cre; Phf6<fl/Y>male mouse; respectively enriching Lin-cells in bone marrow of two male mice, infecting retroviruses overexpressing GFP-JAK3M511I, and transplanting GFP + cells into receptor mice irradiated by a lethal dose through caudal vein injection, so as to construct two T-ALL leukemia mouse models. The model provided by the invention can be rapidly passed in mice, simulates the leukemia process of a T-ALL patient with PHF6 gene and JAK3 gene double mutation, and is used for targeted therapy drug screening and curative effect evaluation.

The Mito-Ob obese mouse model overexpresses the mitochondrial protein prohibitin (PHB). Mito-Ob male mice develop insulin resistance in addition to obesity and they do not develop overt diabetes. It has been discovered that these mice also spontaneously develop nonalcoholic steatohepatitis (NASH) and hepatocarcinogenesis over time. Also described is a mutant Mito-Ob mouse that develops lymphadenopathy and histiocytosis.

The invention relates to an application of fur seal oil which mainly contains EPA, DHA and DPA during the preparing of the medicine for curing sexual disorder, belonging to the field of medicine preparation. The fur seal oil is a fine product obtained through the esterification of the rough fur seal oil and the molecular distillation, wherein, the weight percentages of three omega-3 polyene unsaturated fatty acid are respectively 6.5 to 9.0 percent EPA, 10.5 to 12.5 percent DHA and 4.0 to 8.0 percent EPA. The active ingredient (fur seal oil) of the invention can increase the weight of the gonad of a large male mouse which is weak in sexual ability, increase the plasma stosterone content, prolong the time of the erection of penis, and increase the mating times of the male mouse with the female mouse. Thereby, the invention can improve the erection of penis and better the symptoms of the diseases related to vasoconstriction.