Preparation method, application and evaluation method of collagen-induced arthritis animal model

An animal model, arthritis technology, applied in the biological field, can solve problems such as the inability to effectively prevent the development of the disease

Inactive Publication Date: 2019-11-22
NANTONG UNIVERSITY
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Problems solved by technology

For the clinical treatment of RA, non-steroidal anti-inflammatory drugs, disease-modifying antirheumatic drugs, glucocorticoids a

Method used

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  • Preparation method, application and evaluation method of collagen-induced arthritis animal model
  • Preparation method, application and evaluation method of collagen-induced arthritis animal model
  • Preparation method, application and evaluation method of collagen-induced arthritis animal model

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Embodiment Construction

[0036] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will describe in detail in conjunction with specific embodiments.

[0037] A preparation method of collagen-induced arthritis animal model, specifically comprising the following steps:

[0038] (1) Select 6-8 weeks, male, DBA / 1 mice.

[0039] (2) Raise the mice selected in step (1) in a clean animal room with a temperature of 20-24°C, a relative humidity of 50%-60%, and alternating light and dark for 12 hours. They can eat and drink freely and adapt to the environment for three days. used to prepare models.

[0040] (3) Weigh the chicken Type collagen 100 μg, dissolved in 50 μl of 0.1 mol / L glacial acetic acid, overnight at 4°C; prepare collagen solution A. Wherein, the preparation method of the glacial acetic acid comprises the following steps: dissolving 5 μg of pure glacial acetic acid in 870 ul of double-distilled water, and mi...

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Abstract

The invention provides a preparation method of a collagen-induced arthritis animal model. The method includes the steps that 6-8 week old male DBA/1 mice are selected; the mice selected in step (1) are placed and raised in a clean animal room where the temperature is 20-24 DEG C, the humidity is 50%-60%, and light and dark are alternating every 12 hours, the mice are free to take food and drink water, and after three days of acclimatization the mice are used for preparing the model; a chicken type-II collagen is used for immunizing the DBA/1 mice to induce the CIA model, and the mice are immunized by intradermal injection into the tail bases of the mice; three immunizations are conducted. The method is of great significance for the pathological mechanism study and drug development of arthritis. Meanwhile, an evaluation method of the collagen-induced arthritis animal mode, through arthritis clinical index grading, ankle joint pathology examination, and determination of serum anti-CII-IgG antibody level and other indicators, is used for verifying whether the model is successful and comprehensively evaluating the collagen-induced arthritis animal model.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method, application and evaluation method of a collagen-induced arthritis animal model. Background technique [0002] Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease mediated by immunocompetent cells and various cytokines. The prevalence rate of this disease in my country's population is 0.34%-0.36%, and the incidence rate of women is more than three times higher than that of men. The clinical manifestations of RA are multiple symmetrical arthropathy, joint pain, swelling, stiffness, and dyskinesia. The basic pathological changes are synovitis and progressive joint destruction. The disorder and imbalance of the immune system are the main reasons for the occurrence and development of rheumatoid arthritis. T, B lymphocytes and the cytokines they secrete play an important role in the pathogenesis and progression of RA. For the clinical tr...

Claims

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Application Information

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IPC IPC(8): A01K67/027G01N1/30G01N33/535G01N33/564
CPCA01K67/027A01K2207/10A01K2207/20A01K2227/105A01K2267/0368G01N1/30G01N33/535G01N33/564
Inventor 马颂华陈罡王小琴刘燕
Owner NANTONG UNIVERSITY
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