4 results about "Joint destruction" patented technology

The invention discloses a construction method and application of an overexpression growth inhibition and DNA damage induction gene 45 gamma adenovirus. The construction method comprises the following steps: 1) constructing a recombinant shuttle plasmid containing a GADD45 gamma gene; 2) co-transforming the recombinant shuttle plasmid containing the GADD45 gamma gene and an adenovirus skeleton plasmid into escherichia coli cells, and generating a recombinant GADD45 gamma adenovirus plasmid through homologous recombination; and 3) transfecting the recombinant GADD45 gamma adenovirus plasmid into a 293 cell, packaging the recombinant GADD45 gamma adenovirus plasmid by the 293 cell to obtain adenovirus particles with infection activity, and amplifying and purifying the recombinant GADD45 gamma adenovirus in the 293 cell to obtain the recombinant GADD45 gamma adenovirus. The overexpressed GADD45 gamma adenovirus slows down the disease progress of CIA mice, avoids joint destruction of the CIA mice, provides a new method and a new theory for clinical prevention and diagnosis of RA, and provides a new target for treatment of rheumatoid arthritis and autoimmune diseases.

The invention provides a traditional Chinese medicine composition for treating early rheumatoid arthritis and a preparation method thereof, and the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 8-12 parts of radix bupleuri, 8-12 parts of cassia twig, 8-12 parts of radix stephaniae tetrandrae, 15-25 parts of radix clematidis, 10-20 parts of rhizoma corydalis, 5-15 parts of radix scutellariae, 8-16 parts of radix angelicae, 15-25 parts of radix paeoniae alba and 3-8 parts of liquorice. The traditional Chinese medicine composition has the beneficial effects that the traditional Chinese medicine composition for treating early rheumatoid arthritis is a pure traditional Chinese medicine prescription, is good in clinical curative effect, can promote recovery of joint meridians and collaterals, is small in toxic and side effects, is wide in medicine source, is low in cost, and is suitable for popularization and application. The traditional Chinese medicine composition is of great significance in further improving the clinical curative effect of traditional Chinese medicine on early rheumatoid arthritis and preventing joint damage, deformity or dysfunction, and is suitable for clinical popularization and application.

The application discloses application of isoprenyl cysteine carboxymethyltransferase as a rheumatoid arthritis treatment target in preparation of a rheumatoid arthritis treatment drug. The application finds that the expression of ICMT is down-regulated after NSUN2 is knocked out, further research on the expression level of ICMT in RA FLS finds that the mRNA and protein expression of RA FLS ICMT is obviously up-regulated, the cell migration and invasion ability is reduced after ICMT is knocked down, and the expression of MMPs such as MMP1, MMP3 and MMP9 of RA FLS is obviously inhibited. The above results show that ICMT can regulate the abnormal migration and invasion of RA FLS, and targeting ICMT can become a new means to prevent RA joint destruction. Therefore, the application provides an effective treatment target for RA, and provides a new idea for clinical treatment of RA.

The invention belongs to the technical field of biology, and particularly relates to application of a biomarker for rheumatoid arthritis. The anti-lactylated histone autoantibody and various autoantibodies are found, the level of the anti-lactylated histone autoantibody and various autoantibodies in a serum sample of a rheumatoid arthritis patient is remarkably increased, and compared with a normal person, the anti-lactylated histone autoantibody and various autoantibodies can be used as a biomarker for diagnosis of rheumatoid arthritis. In addition, the level of H3K9la in synovial fibroblasts of rheumatoid arthritis patients is increased, and the H3K9la has the effect of promoting joint destruction. Therefore, the effect of relieving arthritis can be achieved by inhibiting the level of H3K9la, and the H3K9la can be used as a biomarker with the effect of treating rheumatoid arthritis. The invention provides an effective means for diagnosis and treatment of rheumatoid arthritis.