11 results about "Histone" patented technology

The present disclosure includes, among other things, methods, kits, and systems for determining a status of lung cancer. In various embodiments, the present disclosure relates to the use of one or more histone modifications, chromatin accessibility, binding of one or more transcription factors, and / or DNA methylation as features of a status of lung cancer. In some embodiments, differential modifications and / or differential accessibility are detected and quantified at one or more genomic loci in a biological sample, e.g., cell-free DNA (cfDNA), from a liquid biopsy sample obtained or derived from a subject having lung cancer. In various embodiments, the determined status can be used, e.g., to select a treatment for lung cancer and / or to treat lung cancer.

The application discloses a transient transfection method for improving recombinant protein expression of CHO cells, and relates to the technical fields of eukaryotic cell gene transfection and animal cell culture. A cell suspension is obtained by mixing a CHO cell and a serum-free CHO cell basic culture medium; DNA and a transfection reagent are added into the cell suspension to mix a transfection culture, 5-azadeoxycytidine acid solution is added into the transfection culture at 0h-24h to form a transfection system and continue the transfection culture; the transfection system is added with a feeding culture medium at 1d-4d after the transfection culture to carry out suspension culture; the suspension culture is centrifuged at 7d to separate and harvest cell culture supernatant to complete recombinant protein expression. According to the application, 5-azadeoxycytidine acid is added into the transfection culture at a specific concentration, so that the expression of recombinant protein of the CHO cell is significantly improved during the transfection expression.

This invention relates to the fields of gene editing and animal genetic breeding technology, and discloses a precise editing method for key genes regulating lipid metabolism in cattle and sheep. The method includes: constructing a comprehensive lipid metabolism network map integrating transcriptomic, proteomic, and metabolomic data; using a graph neural network to identify key node genes with high flow weights and their negative feedback pathways; simulating cascade reactions after gene perturbation to assess compensatory effects; screening for multi-gene combinations that do not induce significant compensation and can synergistically regulate fat deposition and muscle growth; designing expression vectors containing multiple guide RNA sequences to achieve simultaneous and precise editing; and obtaining edited individuals and validating the phenotype through embryonic or somatic cell nuclear transfer. This invention, through system-level multi-target synergistic editing, avoids metabolic imbalances, improves editing effectiveness, and promotes muscle development while reducing fat deposition, providing a safe and efficient technical path for precision breeding of cattle and sheep.

This invention provides an application of gibberellin in the H3K27me3 modification of NAAT1 or HsfA2d and its role in regulating alkali-heat tolerance. It reveals that active gibberellin is associated with a series of genes, balancing the histone H3K27 trimethylation (H3K27me3) modification levels of NAAT1 and HsfA2d under alkali-heat stress, thereby enhancing the tolerance of gramineous plants to alkali-heat stress. This invention provides an important theoretical basis for breeding plant varieties or other plants with alkali-heat tolerance.

This disclosure includes, in particular, methods, kits, and systems for determining the ER status of cancers, such as breast cancer. In various embodiments, this disclosure relates to the use of one or more histone modifications, chromatin accessibility, binding of one or more transcription factors, and / or DNA methylation as characteristics of the ER status of cancer. In some embodiments, differential modifications and / or differential accessibility are detected and quantified at one or more genomic sites in a biological sample, such as cell-free DNA (cfDNA), obtained from or derived from a liquid biopsy sample from a subject with cancer. In various embodiments, the determined ER status can be used, for example, to select for treatment and / or therapy for cancer, such as breast cancer.

This invention belongs to the field of gene detection technology and discloses a KMB-17 host cell residual DNA analysis and detection kit, method, and application. The method uses the KMB-17 cell genome as a template to design primer pairs and probes, and utilizes qPCR technology to establish a method for analyzing and detecting KMB-17 host cell residual DNA. This detection method has high specificity, high amplification efficiency, high sensitivity, and good reproducibility. The detection kit can rapidly and specifically detect KMB-17 host cell DNA residues at the fg level. It can be used to quantitatively detect the content of host cell residual DNA in intermediate products, semi-finished products, and finished products of various vaccines, recombinant protein drugs, antibodies, cell and gene drugs produced using KMB-17 diploid cells as host cells. This method can serve as a supplementary method for detecting host cell residual DNA.

Monocot plants heterozygous for centromeric histone 3 (CenH3) and optionally expressing gene editing constructs, for use in inducing haploids of a monocot target plant and optionally pass-through gene editing are provided. The monocot haploid inducer plants are typically composed of diploid plant cells having only one allele encoding a functional CENH3 protein. The diploid plant cells can also include, for example, one CenH3 allele encoding non-functional CENH3 protein. In some embodiments, the allele encoding non-functional CENH3 protein is a frameshift mutation, protein null allele, an RNA null allele, or a combination thereof. The monocot haploid inducer plant can also include gene editing machinery, such as a site-directed nuclease and optionally a guide RNA stably expressed by cells of the monocot plant. Methods of inducing formation of a target haploid monocot plant while optionally simultaneously modifying the target monocot plant's genome are also provided.

This invention provides a method for isolating cell nuclei from *Pinus tabuliformis* tissue, relating to the field of plant molecular and cell biology. The method uses *Pinus tabuliformis* callus tissue as explants, releases protoplasts using a specific enzymatic hydrolysis solution, lyses them using a specific lysis buffer, and finally filters and centrifuges to obtain *Pinus tabuliformis* cell nuclei. Using this invention, cell nuclei suitable for single-cell and histone modification library construction and sequencing can be rapidly isolated, providing a foundation for research on single-cell nuclear genetic material transcription analysis, tissue-specific analysis, and cell fate determination in gymnosperms, and offering research ideas for the future use of cell nuclei in coniferous genetic engineering.

This invention discloses a fluorine-nitrogen co-doped carbon dot, its preparation method, and its applications. The fluorine-nitrogen co-doped carbon dot is a nanoparticle constructed using a one-step hydrothermal synthesis method with fluorine-containing and nitrogen-containing precursors. The nanoparticle is spherical with a particle size of 5 nm, and its surface is rich in carboxyl, amino, and hydroxyl functional groups. Its fluorescence quantum yield is not less than 20%, and its emission wavelength is in the 500-550 nm range. It has the characteristic of targeting the H3K36me2 histone modification in tumor cell nuclei, and can achieve specific labeling of tumor cells within 5 minutes. The enrichment intensity in tumor tissue is more than 2.72 times that in normal tissue. The fluorine-nitrogen co-doped carbon dot of this invention is expected to serve as a novel fluorescent labeling probe for tumor identification and imaging, contributing to the early and accurate diagnosis of malignant tumors. It has multiple advantages such as speed, accuracy, safety, and visualization, and has broad application prospects in tumor pathology identification and intraoperative navigation.

Various aspects of the present disclosure provide a method of detecting oncogenic fusions based on ddPCR, and their use in screening and monitoring cancer treatment is disclosed. Described herein is a method for detecting oncogenic fusions involving the histone lysine N-methyltransferase 2A (KMT2A) gene.