257 results about "Histone" patented technology

The present invention relates to compounds useful as inhibitors of one or more histone demethylses. The invention also provides pharmaceutically acceptable compositions comprising compounds of the present invention and methods of using said compositions in the treatment of various disorders.

In recognition of the need to develop novel therapeutic agents and efficient methods for the synthesis thereof, the present invention provides novel compounds of general formula (I):and pharmaceutically acceptable derivatives thereof, wherein R1, R2, R3, n, X and Y are as defined herein. The present invention also provides pharmaceutical compositions comprising a compound of formula (I) and a pharmaceutically acceptable carrier. The present invention further provides compounds capable of inhibiting histone deacetylatase activity and methods for treating disorders regulated by histone deacetylase activity (e.g., cancer and protozoal infections) comprising administering a therapeutically effective amount of a compound of formula (I) to a subject in need thereof. The present invention additionally provides methods for modulating the glucose-sensitive subset of genes downstream of Ure2p. The present invention also provides methods for preparing compounds of the invention.

Disclosed herein are novel approaches to thyroid cancer therapy. These approaches include methods to enhance thyroid specific gene expression, for example methods to enhance expression of thyroglobulin and / or the Na<+> / I<-> symporter in thyroid cancer cells. Enhanced expression of thyroid-specific genes promotes cellular differentiation and reduces biologically aggressive behavior such as invasion and metastasis. In addition, enhanced expression of thyroglobulin and / or the Na<+> / I<31 > symporter increases the ability of thyroid cancer cells to concentrate iodine or iodide, thereby making the cells more susceptible to radioactive iodine therapy. Also disclosed herein are methods for detecting thyroid neoplasms in a subject, by administering a therapeutically effective amount of a histone deacetylase inhibitor, administering a detectable agent whose uptake or concentration in thyroid cells is increased by administration of the histone deacetylase inhibitor, and detecting the detectable agent.

Focused acoustic treatment of samples including a target biomolecule, such as cfDNA, may aid in the recovery of the biomolecule from a sample. cfDNA in a sample, whether chemically stabilized or not, may be linked or otherwise bound to histones or other proteins, e.g., by hydrogen bonds of histones or chaperone proteins to DNA and / or covalent crosslinks of such proteins to the DNA. Focused acoustic energy may remove or disrupt such links, aiding in isolation of the cfDNA from the sample.

The invention relates to a sausage, in particular to a Taiwan-style beef sausage and preparation method thereof. The Taiwan-style beef sausage is prepared from the following raw materials in parts by weight: 40 to 55 parts of beef, 1 to 4 parts of beef tallow, 5 to 17 parts of protein meat, 10 to 25 parts of duck neck skin, 12 to 25 parts of ice water, 0.5 to 2 parts of salt, 0.1 to 0.4 part of tripolyphosphate, 0.001 to 0.015 part of monascus color, 0.1 to 0.2 part of iso-VC sodium, 0.001 to 0.015 part of sodium nitrite, 0.1 to 0.2 part of aminotransferase, 2 to 5 parts of rehydration histone, 5 to 9 parts of white sugar, 0.1 to 0.3 part of white spirit, 7 to 15 parts of cassava starch, 0.5 to 0.7 part of carrageenan, 1 to 2.5 parts of R-type protein, 1 to 2.5 parts of isolated soybean protein, 0.1 to 0.15 part of essential oil, 0.3 to 1 part of ox bone essence, 0.1 to 0.3 part of aroma enhancing juice, 0.1 to 0.3 part of yeast cream, 0.2 to 0.5 part of monosodium glutamate, 0.1 to 0.2 part of white pepper powder, 0.01 to 0.03 part of netmeg powder, 0.02 to 0.07 part of licorice powder, 0.1 to 0.3 part of cassia oil powder, 0.01 to 0.02 part of garlic powder, and 0.015 to 0.03 part of ginger powder; the light sausage product is prepared from the raw materials by weight by the unfreezing of raw meat, trimming, mincing, primary stirring, salting, secondary stirring and filling, cooking in a smoking oven, cooling, shearing and pacing; and the Taiwan-style beef sausage has the advantages of good mouthfeel, full meat granules and low fat, and is health-care food.

The invention provides fusion protein reporter molecules that can be used to monitor protein modifications (e.g., histone modifications) in living cells, and methods of using the fusion reporter molecules for diagnosing protein-modification-associated disorders (e.g. histone-modification-associated disorders). The invention also provides methods of using the fusion protein reporters to identify candidate pharmaceutical agents that effect protein modification in cells and tissues, thus permitting identification of candidate pharmaceutical agents for treatment of protein-modification-associated disorders.

Hyper-inflammatory responses can lead to a variety of diseases including sepsis. It is now shown that extracellular histones released in response to inflammatory challenge are mediators contributing to endothelial dysfunction, organ failure and death during sepsis. As such, they can be targeted pharmacologically by inhibitors, as well as used as biomarkers for prognosis of sepsis and other diseases.

The present invention provides a method of obtaining a gene capable of becoming an index for predicting the efficacy of a histone deacetylase inhibitor, which comprises at least (I) a step of dividing tumor cells into a histone deacetylase inhibitor sensitive tumor cell and a histone deacetylase inhibitor resistant tumor cell, and (II) a step of examining the gene expression pattern of each of the sensitive tumor cell and the resistant tumor cell, and a step of selecting (i) a gene showing high expression in the sensitive tumor cell and low expression in the resistant tumor cell, or (ii) a gene showing low expression in the sensitive tumor cell and high expression in the resistant tumor cell, in order to provide a gene useful for predicting the efficacy, particularly an antitumor effect, of a histone deacetylase inhibitor, for a tumor desired to be treated.

Methods and compositions to increase Agrobacterium transformation efficiency (frequencies) in both dicot and monocot host plants include adding histones to the host plant at most transiently, and using histones and L-cysteine at certain stages in monocot transformation.

A method for promoting wound healing and preventing scar formation in a variety of wounds in skin, mucosa, and cornea. The method comprises administering a therapeutically effective amount of a histone deacetylase inhibitor or a hyperacetylating agent. The histone deacetylase inhibitor or hyperacetylating agent is capable of stimulating multiple cytokines / growth factors in the early phase of wound healing, and suppressing fibrogenic cytokines / growth factors in the late phase of tissue remodeling in the wound site, and is useful in promoting epithelial cell re-growth and reducing excessive collagen accumulation, which results in rapid wound closure with reduced scaring.

The invention discloses a non-induced expression genetic engineering strain and the constructing method and the application. The host cell of the strain is enteric bacilli E.coliBL21, CCTCC M205136, PCR expanding hrp Z gene section to clone to pGEM-T carrier, ferment cutting and bonding by EcoR I / Xho I, inserting the hrp Z gene into the enteric bacilli expression carrier pET-32b(+) lower reaches of thioredoxin, constructing rebuilding expression particle pET-32b-hrp, and transferring into E.coliBL21, filtering to gain positive clone. The non-induced expression rebuilt albumen HrpZ, SDS-PAGE shows that high efficiently expresses APDZ albumen. The rebuilt albumen has good prevention effect to plant disease and could improves the yield.

Expression-enhancing nucleotide sequences for eukaryotic expressions systems are provided that allow for enhanced and stable expression of recombinant proteins in eukaryotic cells. Genomic integration sites providing enhanced expression and methods of use thereof are provided for expression of a gene of interest in a eukaryotic cell. Chromosomal loci, sequences, and vectors are provided for enhanced and stable expression of genes in eukaryotic cells.

The present invention relates to medicinal chemistry, and is especially one kind of hydroxamic acids as histone deacetylse inhibitor, and discloses their preparation process and new intermediate for preparing these compounds. These compounds may be applied in treating diseases caused by disorder of histone deacetylse activity, such as solid tumor, leukemia and nerve degenerative diseases.

A conjugate of a histone moiety covalently attached to a macromolecule-of-interest, in which the histone moiety is transportable through the cell membrane and importable into the cell nuclei, is disclosed. Further disclosed are chemical and recombinant methods of preparing such a conjugate, pharmaceutical compositions containing same and uses thereof for delivering therapeutically active macromolecules into cells. A novel method for quantitatively determining a cytoplasmic uptake and / or a nuclear uptake of a moiety into cells is also disclosed.

Methods for significantly lowering the carbon-14 content of DNA and histones in vertebrates, especially humans, to about 10% to about 98% below normal background levels and thereby reduce chromosomal damage using dietary supplements based on low radiocarbon nucleotides, amino acids, and other DNA and histone precursors. Administration of the supplements during the earliest and / or most active growth staves of life is particularly beneficial. Components of the low radiocarbon supplements can be produced using the waste carbon dioxide effluent from industrial combustion processes, generally considered a greenhouse gas pollutant. Carbon dating methods can be used to monitor the improvements achieved by the methods and compositions of the invention.

The invention belongs to biochemical analysis technology fiels, which in detail relates to a chip enzymolysis microreactor based on functional magnetic microsphere, the preparing method, regeneration method, and its application in protein biochemical analysis. The invention fixes protein enzyme on the surface of magentic microsphere with metal complexation method, and fixes in microchannel of microcurrent chip with its magnetism by using superparamagnetism of magetic microsphere, the protein liquid enters into microchannel under pump pressure for fast and effective enzymolysis, then enzymolysis product flows out of microchannel, and enters into mass analyzer for analysis. The invention aslo regenerates the magnetic microshpere lost enzymatic activity. The invention is characterized by realization of fast protein chip enzymolysis and mass spectrum determination, simple operation, high sensitivity, no protein denaturant, low energy consumption, easy regeneration, suitability for application in fast protein separation and determination in complicate biological sample, which possesses good practical and application value in protein histone research.

The invention belongs to the technical field of plant gene engineering, and more particularly relates to a paddy rice histone demethyl methylase gene, coded protein and the application thereof. The invention provides a new paddy rice histone demethyl methylase gene OsJMJ706, due to inactivation, heteroplasia of rice flowerthe, heteroplasia or deficit of internal and external glumes, as well as lessening of seeds can be caused on the gene, and by being detected by the method of western blot, the gene is proved to have enzyme activity for removing histone H3K9me3 and H3K9me2 locus methylate. The invention provides nucleic acid sequence and protein sequence of the gene as well as the application thereof.

An object of the present invention is to provide a method of comprehensively visualizing a constituent in tumor tissue or the like at a cellular level.The present invention provides a method of forming a two-dimensional distribution image of a target constituent based on information on the mass of constituents of the tissue section wherein, as the internal standard material, any one of actin, tubulin and GAPDH is used in the intracellular region, one of histone and nucleic acid is used in the nuclear region, and one of albumin and cytokine is used in the extracellular region.

The invention discloses a method for preparing a peanut protein at a low temperature and extracting original peanut oil and histone synchronously. The method comprises the following steps: peanuts go through secondary physical pressing in a single-screw oil press to obtain peanut protein powder and crude oil; the peanut protein powder is extruded and puffed in a double-screw extruder to obtain the histone; the crude oil is made into the original peanut oil after the processes of vacuum drying, standing and freezing at a constant temperature, filtering at a constant pressure, and twice finely filtering by a multi-layer vegetable fiber. The method of the invention can synchronously extract the original peanut oil and the histone; the prepared original peanut oil meets the national class 1 pressing standard; the prepared peanut protein structure, that is the histone is characterized by high vegetable protein, high fiber, low fat, no cholesterol, fragrant smell, etc., which can be directly prepared into vegetarian food or food vegetable protein additive material after being further processed. The method can realize the large-scale industrial production.

The invention discloses a method for inducing corn haploids. The method comprises the following steps: pollinating a female parent by a male parent corn haploid induction line with a higher corn haploid inductivity than a corn haploid induction line CAU5, and harvesting hybridized F1 generation grains to obtain corn haploids. A method for realizing the corn haploid induction line comprises the following steps: 1, introducing coding gene of a specific centromere histone mutant into a hybrid first generation plant of a corn inbred line HiIIA and a corn inbred line HiIIB to obtain a transgenic corn plant; and 2, hybridizing the specific histone mutant as a female parent with the CAU5 to obtain an F1 generation, hybridizing the F1 generation as a female parent with the CAU5 to obtain a BC1F1 generation, hybridizing the BC1F1 generation as a female parent with the CAU5 to obtain a BC2F1 generation, and continuously selfing the BC2F1 generation at least five generations to obtain the corn haploid induction line.

Compounds of formula (I) are inhibitors of histone deacetylase activity, and are useful in the treatment of, for example, cancers:wherein Y1 is a bond, —(C═O)—, —S(O2)—, —C(═O)O—, —OC(═O)—, —(C═O)NR3—, —NR3(C═O)—, —S(O2)NR3—, —NR3S(O2)—, or —NR3(C═O)NR5—, wherein R3 and R5 are independently hydrogen or optionally substituted (C1-C6)alkyl, L1 is a divalent radical of formula -(Alk1)m(Q)n(Alk2)p wherein m, n, p, Alk1, Alk2 and Q are as defined in the claims; z is 0 or 1; A represents an optionally substituted mono-, bi— or tri-cyclic carbocyclic or heterocyclic ring system; -[Linker]- represents a divalent linker radical; R is a radical of formula (X) or (Y):wherein R1 is a carboxylic acid group (—COOH), or an ester group which is hydrolysable by one or more intracellular carboxylesterase enzymes to a carboxylic acid group; R4 is hydrogen; or optionally substituted C1-C6 alkyl, C3-C7cycloalkyl, aryl, aryl(C1-C6 alkyl)-, heteroaryl, heteroaryl(C1-C6 alkyl)-, —(C═O)R3, —(C═O)OR3, or —(C═O)NR3 wherein R3 is hydrogen or optionally substituted (C1-C6)alkyl, C3-C7 cycloalkyl, aryl, aryl(C1-C6 alkyl)-, heteroaryl, or heteroaryl(C1-C6 alkyl)-; R41 is hydrogen or optionally substituted C1-C6 alkyl; and B is a monocyclic heterocyclic ring of 5 or 6 ring atoms wherein R1 is linked to a ring carbon adjacent the ring nitrogen shown, and ring B is optionally fused to a second carbocyclic or heterocyclic ring of 5 or 6 ring atoms in which case the bond shown intersected by a wavy line may be from a ring atom in said second ring.

The present invention provides reconstituted histone octamers with multiple modifications (e.g. acetylation of all histones and trimethylation of histone H3K4) assembled onto plasmids for increased transcription post-transfection using our unique bi-lamellar invaginated liposomes (BIVs) to more effectively recruit the transcriptional machinery of human cancer cells post-transfection and substantially increase the production of therapeutic gene products.

The invention relates to a histone-modified chromosome co-immunoprecipitation method applicable in a tissue sample, which comprises the following steps: carrying out tissue sample pretreatment; grinding in an ice bath, filtering with sterile gauze, collecting cells in the filtrate, and treating the suspended cells in an enzyme digestion buffer solution under water-bath conditions; adding micrococcus nuclease into the cells with the enzyme digestion buffer solution, carrying out enzyme digestion, and adding an enzyme digestion termination solution with the same amount as the micrococcus nuclease; carrying out high-frequency ultrasonic treatment and centrifugation in an ice bath to obtain a pulverized chromatin solution; taking ProteinA / G, adding a BSA (bovine serum albumin)-containing PBS (phosphate buffer solution), adding an antibody, incubating, adding the pulverized chromatin solution, and incubating over night; and washing with a high salt solution many times on a magnetic rack, eluting DNA (deoxyribonucleic acid) on the magnetic bead with a TE solution, and purifying with a purification solution, extracting the DNA, and dissolving the DNA in ultrapure water. Compared with the prior art, the method can accurately position the combination site of the histone or transcription factor on the high flux level, thereby enhancing the detection effect of the clinic sample.

The invention discloses a method for cultivating a corn haploid inducer with higher corn haploid inductivity than a corn haploid inducer CAU5. The method comprises the following steps: 1, inducing coding gene of a specific histone mutant into a hybrid first generation plant of a corn inbred line HiIIA and a corn inbred line HiIIB to obtain a transgenic corn plant containing the specific histone mutant; and 2, hybridizing the specific histone mutant as a female parent with the corn haploid inducer CAU5 to obtain an F1 generation, hybridizing the F1 generation as a female parent with the corn haploid inducer CAU5 to obtain a BC1F1 generation, hybridizing the BC1F1 generation as the corn haploid inducer CAU5 to obtain a BC2F1 generation, and continuously selfing the BC2F1 generation five generations to obtain the corn haploid inducer with higher corn haploid inductivity than the corn haploid inducer CAU5.

The invention relates to a kit for detecting antinuclear antibody spectrum related to AIDs. The kit comprises a membrane strip, enzyme-labeled liquid, a substrate and concentrating and washing incubation liquid, wherein the membrane strip is composed of a carrier piece and an antigen band, a critical quality control band and a functional quality control line which are sequentially fixed on the carrier piece. The antigen band is composed of at least two of dsDNA, nucleosomes, SmD1, ribosome P0proteins, histones, U1snRNP, Ro / SS-A(52KD), Ro / SS-A(60KD), La / SS-B, Sc1-70, CENP-B, Jo-1, AMA-M2, PM-Sc1, PCNA, Mi-2 and Ku through independent marking to a nitrocellulose membrane or a nylon membrane. The kit is provided with the ingenious critical quality control band, one critical quality control band can play a role in interpreting two or even more detection bands (antigen bands), and result determination is simpler and more reliable.