149 results about "Assisted reproductive technology" patented technology

The invention belongs to the technical field of assisted reproduction, and particularly relates to a device and a method for gradually adjusting the glucose concentration of an in-vitro embryo culture solution. The device comprises at least two miniature liquid storage bins, wherein each miniature liquid storage bin is filled with a high-concentration glucose solution in advance; the slow release channel is respectively connected with each micro liquid storage bin and the culture solution main container; the culture solution main container is used for accommodating an embryo and a basal culture medium; the control unit is used for sequentially opening the release ports of the miniature liquid storage bins, so that the high-concentration glucose solution sequentially flows into the culture solution main container through the slow release channels. The technical means of staged closed-loop micro sugar control is introduced into the structure and the using method of the device, so that the accurate adjustment of the sugar concentration of the in-vitro embryo culture solution is realized, the requirements of embryos in different development stages can be effectively met, and the embryo culture quality and the subsequent development potential are remarkably improved.

The invention discloses a sperm real-time tracking and segmentation method based on a multi-task cascade analysis network, and the method comprises the following steps: S1, obtaining a sperm bright field image under a microscope, and carrying out the preprocessing of the sperm bright field image; s2, a detection module is adopted to carry out sperm head positioning detection on the preprocessed image, and rapid detection of a high-resolution sperm image is realized through combination of the improved detection module and a self-developed attention mixed feature down-sampler; s3, a sperm head detection result is cut and input to the lightweight real-time segmentation network for real-time segmentation, the model parameter quantity is significantly reduced, and the segmentation precision is improved; and S4, outputting a real-time tracking result to the sperm target in the continuous frames by using a tracking algorithm, and measuring and calculating the activity parameter of the target sperm. Through joint optimization of detection, segmentation and data enhancement modules, an efficient and robust automatic solution is provided for the assisted reproduction technology, and the method can be widely applied to sperm real-time analysis and screening in the assisted reproduction technology.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention relates to the technical field of assisted reproduction polygene detection, and discloses an oocyte occurrence and maturation disorder detection panel, a detection kit and application thereof, and the detection panel comprises mutation genes related to the oocyte occurrence and maturation disorder for detection, copy number variation genes and rearrangement event and deletion genes. According to the application, by detecting high-risk genes and mutation sites, the risk of occurrence and maturation disorder of the oocytes is predicted in combination with clinic, the development potential of the oocytes is evaluated, the in-vitro maturation strategy is optimized, and the clinical outcome of assisted reproduction is improved. The detection panel can be used for efficiently detecting gene mutation which has clinical diagnosis and treatment significance on oocyte occurrence and maturation disorders; the method has important guiding significance on genetic counseling, risk prediction, clinical diagnosis, precise treatment and potential personalized intervention measures of patients suffering from recurrent oocyte occurrence disorder, oocyte maturation disorder and low oocyte maturation rate and patients to be subjected to assisted reproduction technology treatment.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a glucose slow-release structure used in an embryo in-vitro culture solution, a preparation process and a use method. The glucose slow-release structure comprises at least one inner layer containing glucose and a biodegradable material outer layer wrapping the inner layer, and the biodegradable material outer layer comprises a matrix with a plurality of micropores and at least one sealing material layer used for sealing pores of the micropores. According to the invention, gradient release from low sugar in the cleavage stage to high sugar in the blastocyst stage is realized, midway liquid change or artificial sugar supplement is not needed, the operation is obviously simplified, and pollution and mechanical stress risks are reduced.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The invention discloses application of Kitl in promoting meiosis of germ cells cultured in vitro. By adding a small molecule Kit ligand (Kitl), germ cells (including primordial germ cells PGCs, primordial germ cell-like cells PGCLCs and the like) cultured in vitro are promoted to enter and complete the first meiosis earlier stage, meiosis key protein expression is improved, and homologous chromosome association and recombination efficiency is improved. The method has a wide transformation medical prospect in the fields of human assisted reproductive technology and reproductive medicine.

This invention relates to the interdisciplinary field of assisted reproductive technology and artificial intelligence, specifically to a system and method for constructing an embryonic developmental trajectory map that integrates morphological features. By deeply integrating static morphological features with dynamic morphodynamic parameters, a more comprehensive panoramic map of embryonic development with richer information dimensions is constructed, overcoming the one-sidedness of single-dimensional assessment. Automated analysis of the map using an AI model reduces the influence of subjective human factors, enabling the discovery of subtle developmental patterns and potential differences that are difficult to discern with the human eye, significantly improving the accuracy of selecting high-quality embryos. The provided developmental trajectory map allows embryologists to intuitively examine the entire developmental process of the embryo and the performance of each key node, enhancing the interpretability and credibility of the assessment process. Through more scientific embryo selection, it is expected to improve embryo implantation and clinical pregnancy rates, and reduce multiple pregnancy and miscarriage rates.

The application discloses a kind of marker for predicting oocyte copy number variation and application thereof, the marker includes the combination of CLEC11A, P4HB, EFEMP2, IL32, FTL, FLNA, COL6A3, ACPP and APOO protein.By the marker of the application, the expression level of granulosa cell secretory protein can be determined by granulosa cell transcriptome sequencing, and the genomic CNV condition of corresponding oocyte can be inferred.The detection object of the technical solution is discarded granulosa cell in assisted reproductive technology, so it will not have any impact on oocyte, and it is non-invasive;The present study is a transcriptome and methylation group detection from single cell level, with high-throughput characteristics, and all granulosa cells and oocytes are one-to-one matched, which can reflect the difference between single cell level COCs.

The present invention relates to a medium composition for in vitro fertilization and / or in vitro culture comprising a compound represented by chemical formula 1 or a pharmaceutically acceptable salt thereof, and a method using same. The compound or composition suppresses reactive oxygen species and lipid peroxidation in ovarian granulosa cells to preventing cytotoxicity and mitochondrial dysfunction caused by apoptosis and ferroptosis, leading to an improvement in the quality of aged oocytes, and to increase the developmental rate and blastocyst formation rate of pre-implantation embryos, thereby improving the efficiency of in vitro fertilization. In addition, treatment with the medium composition during the vitrification process of in vitro–fertilized embryos improves re-expansion and survival rates, thereby reducing structural and metabolic damage. Accordingly, the present invention can be advantageously applied to an assisted reproductive technology for treating infertility and subfertility, and in vitro fertilization and / or in vitro culture for improving the propagation efficiency of livestock.

The application relates to application of semaglutide in preparation of a medicine for treating or delaying ovarian aging, and through a natural aging mouse model, a cell aging model and transcriptome analysis, it is first proved that semaglutide can significantly increase the number of follicles and reduce the level of aging markers, and from a molecular level, it is revealed that the mechanism of realizing multi-target point delay of ovarian aging is that semaglutide realizes multi-target point delay of ovarian aging through regulating an adipocyte cytokine signal pathway, inhibiting excessive cell division and regulating an NF-kappa B signal pathway. As a marketed drug, semaglutide has clear safety, a long half-life and high patient compliance, and has a significant clinical conversion prospect. The application breaks through the limitation that existing hormone replacement therapy and assisted reproductive technology can only treat symptoms or solve the problem of fertility, provides a first drug intervention strategy which can fundamentally delay the process of follicle depletion, and fills the blank in the field of ovarian aging treatment.

The invention discloses application of rutin hydrate in preventing and treating repeated embryo planting failure, and belongs to the technical field of assisted reproduction. It is found for the first time that CD36 is a key membrane protein for lipid transport and uptake of EECs, rutin hydrate competitively inhibits combination of CD36 and natural ligands of CD36, abnormal lipid excessive uptake is remarkably blocked, lipid accumulation and lipid toxicity damage in cells are effectively relieved, and the effect of inhibiting EECs lipid transport and uptake is achieved. And finally, the endometrial receptivity defect related to RIF is reversed, and the embryo adhesion and implantation capability is remarkably improved. Therefore, rutin hydrate provides a brand new strategy for developing targeted therapy drugs for RIF, especially for lipid metabolism disorder type endometrial receptivity badness, and has remarkable clinical application and transformation prospects.

The invention provides an embryonic development evaluation method and device, electronic equipment and a storage medium, and relates to the technical field of assisted reproduction. The method comprises the following steps: acquiring an embryo image of a to-be-evaluated embryo, and extracting an inner cell mass image from the embryo image; traversing the internal cell cluster image, and determining a pixel distribution index corresponding to the internal cell cluster image; determining at least one feature parameter of the internal cell mass image according to a pixel distribution index corresponding to the internal cell mass image; and determining an evaluation result of the to-be-evaluated embryo according to each characteristic parameter of the inner cell mass image. According to the method, pixel traversal is carried out on an extracted internal cell cluster image to obtain position indexes of a plurality of center pixel points so as to obtain a pixel distribution index, and feature mining is carried out based on pixel relative position difference information indicated by the pixel distribution index so as to obtain multiple feature parameters capable of representing texture information of the internal cell cluster image. Therefore, the method is used for evaluating embryonic development, and the accuracy and credibility of an evaluation result are improved.

The invention discloses application of spermidine in in-vitro culture of human unfrozen oocytes and embryos. By adding the combination of spermidine, melatonin and astaxanthin into the thawed oocyte resuscitation culture solution and the embryo in-vitro culture solution, the mitochondrial autophagy and mitochondrial functions of the thawed oocytes are improved, so that the in-vitro culture effect of the thawed oocytes and embryos is improved, and the success rate of an assisted reproduction technology is increased.

This image is an information display image used for observing sperm in assisted reproductive technology. This image includes an object (information display section) that is displayed according to the position of the sperm and displays information about the sperm, and an object (display information selection section) for selecting information about the sperm to be displayed by the information display section. The information display section displays information about the state of the sperm, for example, using color or brightness. The display information selection section is an operation section that enables selection of information about the sperm to be displayed by the information display section. Image 1 showing the changed state is an image showing the state in which the information displayed by the information display section in the image diagram has changed over time, etc. Image 2 showing the changed state shows that it is also possible to prevent the information display section from being displayed by selecting the display information selection section.

This utility model discloses an improved semen DNA collection card for assisted reproductive technology, belonging to the field of medical supplies technology. It includes a folding card with an upper cover, a lower cover, and a bottom surface. The bottom surface is located between the upper and lower covers. The lower cover has a sampling filter paper surface, and a PVC diaphragm is provided in the middle of the sampling filter paper surface to divide it into a pre-processing semen collection area and a post-processing semen collection area. This utility model stores the patient's unprocessed semen in the pre-processing semen collection area and the processed semen in the post-processing semen collection area. The PVC diaphragm between the two areas prevents interference and cross-contamination between the two semen samples, facilitating DNA comparison before and after semen processing. The folding card also facilitates transportation and transfer, allowing for the storage of both pre- and post-processing semen within a single card, which is beneficial for clinical research.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

The invention provides an integrated frozen carrying rod and an embryo sample storage and taking method, and belongs to the technical field of assisted reproduction. Comprising a carrying rod body and a sheathing canal, and a carrying rod sheet is arranged at one end of the carrying rod body; one end of the sheathing canal is coaxially connected with a protective sleeve, a sealing metal ring is arranged at the end, away from the sheathing canal, of the protective sleeve, a guide sinking groove is formed in the side wall of the sheathing canal in the axial direction, one end of the guide sinking groove is communicated with the other end of the sheathing canal, and the other end of the guide sinking groove is communicated with the protective sleeve. The carrying rod sheet is telescopically arranged in the protective sleeve. The difficulty of sample vitrification and storage operation by using the freezing carrying rod can be reduced, and the operation risk is reduced while the operation efficiency is improved.

The present invention relates to the identification and / or selection of sperm, and in particular a means for identifying and / or selecting the fittest sperm for use in assisted reproductive technologies.

The present application relates to the technical field of biological medicine, and more particularly to a culture solution, a kit and an application for in-vitro maturation of aged oocytes. Through in-vitro experiments, the present application adds exogenous stromal cell-derived factor 1, establishes a culture system for in-vitro maturation of aged oocytes, and reveals the molecular mechanism of stromal cell-derived factor 1 playing a role through autophagy, thereby providing a new solution for improving the fertility of aged women and improving the success rate of assisted reproductive technology, and having important clinical application value.

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

This invention discloses a method for predicting the outcome of in vitro fertilization-embryo transfer (IVF-ET) based on static embryo images, used to distinguish between biochemical pregnancy and clinical pregnancy. It belongs to the field of medical artificial intelligence and assisted reproductive technology and is applicable to single embryo transfer (SET) and dual embryo transfer (DET) scenarios. The method includes: acquiring a static image of at least one embryo to be transferred; preprocessing and standardizing the embryo image, and then inputting it into a feature extraction model based on a convolutional neural network. The model uses DenseNet-169 as the backbone network and combines it with a Feature Pyramid Network (FPN) for multi-scale feature fusion. When the input is a dual embryo image, a Trophectoderm–Inner Cell Attention (TEIC-Attn) attention module is introduced to adaptively weight and fuse the features of the two embryos to characterize the contribution of different embryos to the final transfer outcome. When the input is a single embryo image, the prediction is completed based on the corresponding embryo features. The model ultimately outputs a probability prediction result of whether the IVF treatment outcome is a biochemical pregnancy or a clinical pregnancy. Furthermore, this invention combines a gradient-based class activation mapping method to generate interpretable visual heatmaps, highlighting key morphological regions closely related to embryo transfer outcomes, such as the inner cell mass and trophectoderm, thereby providing clinicians and patients with intuitive and reliable decision-making support. This invention enables unified prediction and interpretation of single and double embryo transfer outcomes without relying on time-series data, improving the accuracy and interpretability of in-vitro fertilization treatment decisions and demonstrating promising clinical application prospects.

The invention relates to the use of carbon monoxide for preserving gametes. Means and methods for preserving gametes are provided. The method of the invention comprises the steps of a) providing a gamete or a sample comprising said gamete in a container, and b) contacting said gamete in said container with carbon monoxide. The invention also provides the use of carbon monoxide in the treatment and / or prevention of diseases, such as diseases caused by or associated with elevated DNA cleavage, redox potential of gametes, and / or diseases of gametes caused by or associated with elevated ROS levels. Also provided are uses of carbon monoxide for the prevention of congenital abnormalities and / or aneuploidy, as well as for the treatment of diseases caused by and / or associated with elevated DNA cleavage and / or redox potential of gametes. The invention also relates to a method of treating congenital abnormalities or aneuploidy comprising contacting carbon monoxide gas with gametes of a patient in need thereof. Thus, the means, methods and uses of the present invention ensure that gametes are preserved in a functional / intact state, thereby ensuring maintained and / or improved gamete quality. Thus, such carbon monoxide treated / carbon monoxide exposed gametes are particularly useful in reproductive techniques, such as in vitro reproductive techniques, including in human assisted reproductive techniques and / or in animal (artificial) insemination techniques.

This invention relates to the field of image analysis technology, specifically to a real-time monitoring and culture system for embryonic development during assisted reproductive technology. It includes: an image data acquisition module for acquiring grayscale images of embryos; an image information content analysis module for determining the splitting information content and sharpness information content of each embryo grayscale image based on changes in the number of edge pixels and differences in pixel grayscale values; a target embryo image screening module for screening target embryo images based on the differences in splitting information content and sharpness information content of the embryo grayscale images; and an embryonic development monitoring module for comparing the similarity features of edge lines in all target embryo images to identify high-information-content embryo images for embryonic development monitoring. This invention can accurately screen embryo images with high reference value, thereby reducing data redundancy and improving the accuracy of embryonic development monitoring.

This invention discloses a microfluidic chip for three-stage non-destructive sperm screening and its usage method. The chip includes a substrate, a sample inlet channel layer, a sample chamber layer, a recovery chamber layer, and a screening filter membrane. The sample inlet channel layer has an inlet channel and an overflow channel. The sample chamber layer has an annular sample chamber, an inlet orifice, and an overflow orifice. The recovery chamber layer is divided into an outer recovery chamber and an inner recovery chamber by a dike. An inducing agent patch is located in the center of the inner recovery chamber. The screening filter membrane is sandwiched between the sample chamber layer and the recovery chamber layer. In use, after injecting a sperm sample, initial motility screening is achieved through the screening filter membrane. Secondary chemotactic screening is completed through an inducing agent concentration gradient. Tertiary adaptive screening for swimming direction is completed using the chemotactic channel. Finally, high-quality sperm are obtained from the inner recovery chamber. This invention achieves multi-parameter graded screening, effectively avoiding sperm damage, improving sperm recovery quality and screening efficiency, and is applicable to various assisted reproductive technologies, helping to improve conception success rates.

The invention relates to the technical field of artificial intelligence in assisted reproduction, in particular to an embryo optimization model construction method, device and equipment and a storage medium. Comprising the following steps: acquiring video data of embryonic development from a plurality of fertilized eggs to a blastocyst stage based on a delayed photography embryo culture technology; constructing time sequence image data according to the video data; the time sequence image data comprises multiple frames of continuous images; performing feature extraction on each frame of image of the time sequence image to obtain a high-dimensional feature corresponding to each frame of image; determining a comparison difference feature with the corresponding reference sample feature, performing fusion, and constructing a fusion feature corresponding to each frame of image; constructing a feature sequence of the target embryo development according to the fusion features; and acquiring a trained embryo quality grading model according to a time dependency relationship of the feature sequence for capturing embryo development. The model provided by the invention can optimize the utilization rate of embryos, reduce unnecessary repeated ovulation promoting cycles, and promote the development of reproductive medicine towards the precision direction.

This invention relates to the fields of biomedical engineering and embryo quality assessment, and particularly to a biomarker for assessing the quality of IVM-derived embryos and its application. The biomarker includes time point parameters t2 and t9 of the IVM-derived embryos, and time interval parameters S2, t9–t7, t9–t8, or tSB–t9. A method for assessing the quality of IVM-derived embryos based on the aforementioned biomarker has been established. This method combines non-invasive temporal imaging technology with morphodynamic multi-parameter analysis, providing a reliable tool for assessing the blastocyst formation potential and chromosomal euploidy of IVM-derived embryos in clinical practice, thus potentially expanding the application of IVM technology in assisted reproductive technology.

The invention relates to the technical field of assisted reproduction, and discloses a microinjection device and an operation method, the microinjection device comprises a handle, a rotary vibration shaft, a piezoelectric vibrator and a micromanipulation needle, the piezoelectric vibrator is tangentially arranged between the handle and the rotary vibration shaft, and electric energy is converted into circumferential high-frequency micro vibration of the rotary vibration shaft. The circumferential vibration is transmitted to the micromanipulation needle connected with the front end, so that the unique fish-mouth-shaped end face of the micromanipulation needle penetrates through the cells in a high-frequency rotary cutting mode, and extrusion damage to the oocytes caused by traditional axial puncture is remarkably reduced. The invention further discloses a gallium-based alloy which is high in density and better in biocompatibility and serves as an operation medium, and the transmission efficiency of vibration energy is improved. The invention also provides a method for pre-filling the microinjection needle by utilizing the physical characteristics of low-temperature curing and normal-temperature liquefaction of the gallium-based alloy and adopting cold chain transportation, so that the product can be used immediately after being opened, the clinical operation steps are obviously simplified, the operation threshold is reduced, and the microinjection efficiency and success rate are improved.

The present application relates to the field of biomedical technology, and more particularly to a prediction model for embryo quality outcome of women of childbearing age undergoing assisted reproductive technology; the present application is based on clinical and laboratory data of women of childbearing age undergoing assisted reproductive technology, uses light GBM multi-factor analysis to screen the key factors of clinical core parameters affecting women of childbearing age undergoing assisted reproductive technology, and performs spear correlation analysis with each clinical core parameter to obtain core factors affecting embryo quality outcome of women of childbearing age undergoing assisted reproductive technology, and to construct a model capable of accurately predicting embryo quality outcome, which can accurately predict the positive influencing factors of embryo quality, effectively reduce the psychological burden of a part of ART pregnant patients, so as to make clinical intervention more targeted, more conducive to the rational allocation of clinical resources, and suitable for large-scale popularization and application.

Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.