3 results about "Sperm dna" patented technology

The application discloses a preparation of a biological memristor and application of the biological memristor in sperm DNA breakage detection, and takes supernatant of a sample to be detected, and drops the supernatant on the surface of the memristor; after the memristor is connected with a detection platform, different voltage corresponding electrical signals are outputted, and the normal sperm and the sperm with DNA breakage are distinguished. The application has the characteristics of simple detection process, low cost, high sensitivity and fast response, and can quickly distinguish whether sperm DNA sequence breakage exists in sperm cells of a male patient with infertility.

The invention relates to an improved method for analysing sperm DNA structure, by means of which it is possible to reduce the time required to process samples by 80%, optimising every stage of the process. This effect is achieved through a synergetic improvement in each step of the process. The formation of microgels is accelerated with cold metal plates (2–8°C) and using 0.8%-1.5% agarose. DNA denaturation is carried out using HCl (100 mM). The deproteinisation solution contains DTT (0.22 M), NaCl (2.75 M), detergents of the alcohol ethoxylate (0.044 M) and SDS (0.0014 M) type. Fixation is carried out using 96% ethanol at a low temperature. The staining process uses a panoptic dye, improving the quality of the images. The invention not only speeds up the analysis by reducing the time required by 80%, but also enables faster and more accurate diagnoses, thanks to the high quality of the images obtained.

This invention, entitled "A Fertilization Culture Medium, Its Preparation Method, and Its Application," belongs to the field of assisted reproductive technology. The technical problem it aims to solve is that existing technologies, due to the generation of sperm DNA fragments during the culture process, lead to a decrease in overall sperm quality, limiting fertilization rate and blastocyst formation rate. This invention proposes a fertilization culture medium that can improve overall sperm quality and thus increase fertilization rate. The key technical points are: a fertilization culture medium comprising the following components: sodium chloride, potassium chloride, alkali metal dihydrogen phosphate, calcium chloride, magnesium sulfate, sodium bicarbonate, sodium lactate, sodium pyruvate, glucose, fructose, disodium edetate, gentamicin, human serum albumin, alanylglutamine, taurine, non-essential amino acids, and testosterone.