77 results about "Sperm dna" patented technology

The invention discloses a preparation method and application of an H1, H2 or J type astaxanthin aggresome water dispersion system. Natural biological macro chitosan and special molecular structures offish sperm DNA are adopted by astaxanthin aggresome water dispersion systems with three colors to induce the formation and stabilizing of an astaxanthin polymer through physical interaction among macro molecules combining with a solvent and a salt ion effect. Ethanol with low toxicity is taken as the astaxanthin good solvent, the organic solvent can be completely removed in the later period of the preparation process and can be further enriched and recycled to facilitate clean production and the reduction of cost. The H1, H2 or J type astaxanthin aggresome water dispersion system can be controlled by regulating technological parameters to allow the developing range of astaxanthin waterborne product to be expanded to yellow, orange and pink, and a stable aggregation state and developing effect of the astaxanthin are maintained in concentration, dehydration and reconstitution process. The preparation technology of the three kinds of the astaxanthin aggresome water dispersion systems issimple, the reaction conditions are mild, the production cost is low, the technology is green and has no pollution, and therefore industrial popularization is convenient.

The invention relates to a raman spectrum-based fast measurement method for a motile sperm deoxyribonucleic acid (DNA). The invention adopts the following technical scheme: collecting a semen sample by a test tube; naturally liquefying semen in a room-temperature environment; absorbing the liquefied semen and adding to a centrifugal tube containing density gradient centrifugate; absorbing upper seminal plasma after centrifuging, discarding, then adding a PBS buffer solution and centrifugally washing again; absorbing supernatant, discarding, adding the PBS buffer solution to prepare a sperm suspension, and incubating; attaching a metal bottom plate to a culture dish; adding the PBS buffer solution, and absorbing the incubated sperm suspension, soaking in the culture dish, wherein the sperm swims outside and contacts the metal bottom plate; setting a spectrum response range, integrating time and the excitation light wavelength, so as to obtain raman spectrum data from the head part of the sperm. The motile sperm swimming out from the pointed-end part of a sucker can be attached to the metal bottom plate by operation of a thin tube pipette, and the damage condition of the sperm DNA is evaluated by measuring the sperm of which the head is attached to the metal bottom plate.

A method for separating non-human mammalian XY sperm, based on the principle that the DNA content of mammalian X sperm is more than that of Y sperm, the sperm DNA is stained with fluorescent dye Hoechst33342 and then separated by flow cytometry. The present invention improves the accuracy of sperm separation and the vitality of sperm after separation while ensuring the efficiency of separating XY sperm by improving the preservation method before sperm separation, the diluent of sperm, the sheath fluid and receiving liquid of separated sperm, and is suitable for The large-scale commercial separation and production of XY sperm of non-human mammals such as cattle, buffalo, and goats can be combined with new animal breeding technologies such as artificial insemination and in vitro fertilization to greatly improve the efficiency of animal husbandry production.

An isolated nucleic acid, a recombinant protein encoded thereby and uses thereof. The isolated nucleic acid including (a) a polynucleotide at least 60% identical to SEQ ID NOs:1, 3, 5 or portions thereof as determined using the Bestfit procedure of the DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the university of Wisconsin (gap creation penalty-50, gap extension penalty-3); (b) a polynucleotide encoding a polypeptide being at least 60% homologous with SEQ ID NOs:2, 4, 6 or portions thereof as determined using the Bestfit procedure of the DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the university of Wisconsin (gap creation penalty-50, gap extension penalty-3); or (c) a polynucleotide hybridizable with SEQ ID NOs:1, 3, 5 or portions thereof at 68° C. in 6xSSC, 1% SDS, 5x Denharts, 10% dextran sulfate, 100 mug / ml salmon sperm DNA, and <32>p labeled probe and wash at 68° C. with 3xSSC and 0.1% SDS.