77 results about "Sperm dna" patented technology

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformly stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.

This invention is a method of separating mammal XY sperm. Fluorescence colorant Hoechst33342 is used to color sperm DNA and then they are separated by flow cytometry according to theory of DNA content of X sperm is more than Y sperm of mammal. Preservation method, sperm diluent, sheath liquor and acceptance liquor used for separating sperm before sperm separating is improved to confirm improve the separation accuracy rate and activity after separation at the same time of ensuring separating XY sperm efficiency. It is fit for big scale commercialization separating generation of XY sperm of scalper, buffalo, goat and other mammal. It can be bonded to artificial insemination and external insemination and other animal breeding new techniques, it greatly improved the efficiency of animal husbandry production.

The invention relates to a method for detecting sperm DNA fragments, comprising the following steps: 1) adjusting sperm concentration; 2) completely melting low melting point gel, and balancing for standby use; 3) mixing the sperm and the gel following proper proportion; 4) absorbing the obtained suspension to solidoid enveloped with high-melting point gel, and adding a cover plate to the suspension to solidify the suspension until the temperature thereof is 2-8 DEG C after standing; 5) removing the cover plate and soaking the enveloped solidoid horizontally into denatured liquid for reaction under the condition of the temperature being lower than the temperature of the low melting point gel; 6) transferring the enveloped solidoid to solution for reaction under the condition of the temperature being lower than the temperature of the low melting point gel; 7) washing the enveloped solidoid; 8) carrying out continuous dehydration following ethanol concentration sequence from low to high, and drying in the air; 9) dyeing; and 10) counting incidence rate of the DNA fragments under a microscope. The device for detecting sperm DNA fragments comprises an enveloped solidoid, low melting point gel aqueous solution, denatured liquid and washing liquid. The outstanding technological effects are: the result is easy to be judged, the operation is easy and clinical promotion and application are easy.

The invention discloses a preparation method and application of an H1, H2 or J type astaxanthin aggresome water dispersion system. Natural biological macro chitosan and special molecular structures offish sperm DNA are adopted by astaxanthin aggresome water dispersion systems with three colors to induce the formation and stabilizing of an astaxanthin polymer through physical interaction among macro molecules combining with a solvent and a salt ion effect. Ethanol with low toxicity is taken as the astaxanthin good solvent, the organic solvent can be completely removed in the later period of the preparation process and can be further enriched and recycled to facilitate clean production and the reduction of cost. The H1, H2 or J type astaxanthin aggresome water dispersion system can be controlled by regulating technological parameters to allow the developing range of astaxanthin waterborne product to be expanded to yellow, orange and pink, and a stable aggregation state and developing effect of the astaxanthin are maintained in concentration, dehydration and reconstitution process. The preparation technology of the three kinds of the astaxanthin aggresome water dispersion systems issimple, the reaction conditions are mild, the production cost is low, the technology is green and has no pollution, and therefore industrial popularization is convenient.

The invention relates to a raman spectrum-based fast measurement method for a motile sperm deoxyribonucleic acid (DNA). The invention adopts the following technical scheme: collecting a semen sample by a test tube; naturally liquefying semen in a room-temperature environment; absorbing the liquefied semen and adding to a centrifugal tube containing density gradient centrifugate; absorbing upper seminal plasma after centrifuging, discarding, then adding a PBS buffer solution and centrifugally washing again; absorbing supernatant, discarding, adding the PBS buffer solution to prepare a sperm suspension, and incubating; attaching a metal bottom plate to a culture dish; adding the PBS buffer solution, and absorbing the incubated sperm suspension, soaking in the culture dish, wherein the sperm swims outside and contacts the metal bottom plate; setting a spectrum response range, integrating time and the excitation light wavelength, so as to obtain raman spectrum data from the head part of the sperm. The motile sperm swimming out from the pointed-end part of a sucker can be attached to the metal bottom plate by operation of a thin tube pipette, and the damage condition of the sperm DNA is evaluated by measuring the sperm of which the head is attached to the metal bottom plate.

The present invention discloses food containing haematococcus pluvialis and trehalose. The food contains the haematococcus pluvialis and trehalose, wherein a mass ratio of the haematococcus pluvialisto the trehalose is (100-800):(500-1,800). The food can increase spermatogenesis, promote sperm proliferation and maturity, energize sperms, increase sperm vitality, protect integrity of sperm cell membranes, increase stability of sperm DNA, comprehensively improve quality of semen and receive satisfactory results.

A method for separating non-human mammalian XY sperm, based on the principle that the DNA content of mammalian X sperm is more than that of Y sperm, the sperm DNA is stained with fluorescent dye Hoechst33342 and then separated by flow cytometry. The present invention improves the accuracy of sperm separation and the vitality of sperm after separation while ensuring the efficiency of separating XY sperm by improving the preservation method before sperm separation, the diluent of sperm, the sheath fluid and receiving liquid of separated sperm, and is suitable for The large-scale commercial separation and production of XY sperm of non-human mammals such as cattle, buffalo, and goats can be combined with new animal breeding technologies such as artificial insemination and in vitro fertilization to greatly improve the efficiency of animal husbandry production.

The invention provides a multifunctional sperm quality analysis system. The multifunctional sperm quality analysis system comprises sperm routine computer-aided sperm analysis (CASA) and image analysis, the CASA is used for analyzing a sample video recorded through a CCD camera or video inputted into the local of an analysis host to calculate the quantity, the concentration, the vitality and the motion trail of sperms of the sample; the image analysis is used for analyzing sperm functions and comprises sperm DNA fragment detection, sperm nucleoprotein detection and sperm morphological detection, and pictures taken through the CCD camera or pictures inputted to the local of the analysis host are analyzed. The problems that existing seminal fluid analysis operation is tedious, human factorsare uncontrollable, and the inspection cost is high are solved.

The invention relates to the field of fish sperm preservation solutions, in particular to a pseudobagrus sperm preservation solution for solving the problem of pseudobagrus large scale reproduction and a preparation method of the pseudobagrus sperm preservation solution. The sperm preservation solution is prepared from sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium bicarbonate, glucose and penicillin and is prepared by weighing the raw materials, adding the raw materials in a plastic bottle or a glass bottle, and preparing into a solution with distilled water. The prepared sperm preservation solution is subjected to sperm chromatin detection with a flow cytometer, spermatids are circled on an FSC / SSC graph, and a door is set on an FL1(FITC) / FL3(ECD) scatter diagram for analysis; and a DNA fragment index at is calculated through a formula to evaluate sperm DNA quality. The sperm preservation solution provided by the invention can be preserved for 10 days in a refrigeration tank at the temperature of 4 DEG C, 98 percent or more of sperms keep exuberant vigor and can still be taken as sperms for semination, and the fertilization rate is 95 percent orabove.

The invention provides a method and a kit for detecting sperm DNA fragment rate. According to the method and the kit provided by the invention, RNA in a sperm sample is digested by RNA enzyme based onflow cytometry, so that mixed signals brought by sperm RNA are eliminated, the detection result is more accurate, stable and reliable, the repeatability is good, and the clinical popularization is easy.

An isolated nucleic acid, a recombinant protein encoded thereby and uses thereof. The isolated nucleic acid including (a) a polynucleotide at least 60% identical to SEQ ID NOs:1, 3, 5 or portions thereof as determined using the Bestfit procedure of the DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the university of Wisconsin (gap creation penalty-50, gap extension penalty-3); (b) a polynucleotide encoding a polypeptide being at least 60% homologous with SEQ ID NOs:2, 4, 6 or portions thereof as determined using the Bestfit procedure of the DNA sequence analysis software package developed by the Genetic Computer Group (GCG) at the university of Wisconsin (gap creation penalty-50, gap extension penalty-3); or (c) a polynucleotide hybridizable with SEQ ID NOs:1, 3, 5 or portions thereof at 68° C. in 6xSSC, 1% SDS, 5x Denharts, 10% dextran sulfate, 100 mug / ml salmon sperm DNA, and <32>p labeled probe and wash at 68° C. with 3xSSC and 0.1% SDS.