16 results about "Sperm dna" patented technology

The invention discloses an efficient extraction method of blue fox sperm DNA, and belongs to the technical field of molecular biology. Aiming at the structural characteristics that chromatin of the blue fox sperms is highly condensed, histone is replaced by protamine, and a stable nucleoprotein complex is formed through an intermolecular disulfide bond, the invention provides an efficient DNA extraction method suitable for the blue fox sperms. According to the method, based on the synergistic effect of three chemical reagents, namely SDS, PK and DTT, full lysis of the sperm cells of the blue foxes is achieved, and then efficient enrichment of sperm DNA of the blue foxes can be achieved by combining a conventional DNA extraction method. According to the efficient extraction method of the blue fox sperm DNA provided by the invention, the yield and integrity of the blue fox sperm DNA are remarkably improved, and the limitation that sperm chromatin is difficult to effectively lyse by a conventional method is overcome; the method provides reliable technical support for research on genetic diversity evaluation, population management, molecular breeding and the like of the blue foxes, and has the potential of popularization and application in other high-condensation sperm species.

The utility model provides a seminal fluid treatment device which comprises a base, a seminal fluid injection port arranged at the first end of the base, a sperm recovery pool arranged at the second end of the base, a seminal fluid storage bin and a sperm upstream channel, the seminal fluid storage bin and the sperm upstream channel are arranged in the base, and the first end of the seminal fluid storage bin is communicated with the seminal fluid injection port. The second end of the semen storage bin is communicated with the first end of the sperm upstream channel, the second end of the sperm upstream channel is communicated with the sperm recovery pool, and the first end and the second end of the sperm upstream channel are both provided with filter membranes. According to the utility model, on the basis of the characteristic that sperms swim upwards, centrifugal operation is not needed in the semen treatment process, so that the damage to sperm DNA and organelles is reduced to the greatest extent; the operation process is simple and rapid, the technical requirements for operators are low, and standardization is easier; in addition, less culture medium is needed in the operation process, a centrifugal machine and the like do not need to be purchased, and the use cost is low.

The utility model relates to an optimization device in the aspect of micro-fluidic sperm optimization, belongs to the technical field of medical instruments, and solves the problem of low concentration of high-quality sperms after sperm optimization in the prior art. The fallopian tube simulating device comprises a collecting pool and a screening piece, the screening piece is connected with the collecting pool, a screening channel is arranged on the screening piece and provided with an inlet end and an outlet end, the inlet end can be soaked in original semen, the outlet end is connected with the collecting pool, and the fallopian tube is simulated through the screening channel. And performing sperm screening by using the boundary tracking navigation capability of the sperms. According to the sperm screening device, the screening piece is infiltrated into the original sperm, the fallopian tube is simulated through the screening channel, sperm screening is carried out through boundary tracking navigation capacity of sperms, operation is simple, centrifugal operation is not needed in the screening process, sperm DNA cannot be damaged or broken due to peroxidation, the concentration of screened high-quality sperms is improved, and then the fertilization rate is increased.

The present invention refers to a biomass of T. chuii and a pharmaceutical composition comprising a biomass of T. chuii for use in the treatment of infertility in a male subject. It also refers to a method for increasing the quality of the semen of a male subject having normozoospermia, not having hypospermia and not having a disorder characterized by a high percentage of sperm DNA fragmentation (SDF), that comprises the administration of a biomass of T. chuii.

The application discloses a preparation of a biological memristor and application of the biological memristor in sperm DNA breakage detection, and takes supernatant of a sample to be detected, and drops the supernatant on the surface of the memristor; after the memristor is connected with a detection platform, different voltage corresponding electrical signals are outputted, and the normal sperm and the sperm with DNA breakage are distinguished. The application has the characteristics of simple detection process, low cost, high sensitivity and fast response, and can quickly distinguish whether sperm DNA sequence breakage exists in sperm cells of a male patient with infertility.

The invention discloses a kit and a method for combined detection of sperm cell active oxygen and DNA fragment index, and belongs to the technical field of reproductive biology and clinical diagnosis. The kit comprises reactive oxygen dye, acridine orange dye, acidizing fluid and buffer solution. According to the invention, a specific fluorescent dye is combined with a sperm DNA fragment detection method to obtain a method capable of jointly detecting the sperm cell DNA fragment index and the active oxygen, the detection method can be used for simultaneously detecting in the same tube buffer system, and signals among detected items are not influenced. By adopting the detection method disclosed by the invention, the sperm cells with high active oxygen, high DFI and extremely poor quality (ROS + DFI +) and low active oxygen, low DFI and extremely good quality (ROS-DFI-) can be obviously detected, and clinical research and screening of sperm cells with higher quality and distinguishing of poorer sperm cells can be facilitated.

This invention discloses a traditional Chinese medicine compound composition for protecting sperm DNA integrity, its preparation method, and its application. The composition comprises the following components by mass: 10-30 parts Codonopsis pilosula, 10-20 parts Citrus reticulata, 10-30 parts Taraxacum mongolicum, 10-30 parts Agrimonia pilosa, 10-30 parts Astragalus membranaceus, 10-30 parts Rhodiola rosea, 6-10 parts Astragalus complanatus, 6-10 parts Morus alba, 6-10 parts Schisandra chinensis, 6-10 parts Curcuma zedoaria, 6-10 parts Carthamus tinctorius, and 6-10 parts Citrus reticulata seed. This traditional Chinese medicine compound composition can tonify the spleen and kidneys, clear heat and detoxify, invigorate blood circulation and regulate qi, and balance the yin and yang of kidney essence. It effectively protects sperm DNA integrity and reduces the increase in sperm DNA fibrillation (DFI) caused by sperm DNA damage, demonstrating definite clinical efficacy. The traditional Chinese medicine compound composition of this invention is convenient to take in clinical use, has good patient tolerance, and no adverse reactions have been found with long-term oral administration. After being made into a standardized traditional Chinese medicine preparation, it can further promote the standardized treatment and use of traditional Chinese medicine.

The application provides application of pyridoxamine in sperm of oligospermia, preferably and / or in a freezing and resuscitation operation, pyridoxamine is added in a sperm operation liquid or a semen freezing liquid, and the following shortcomings of patients with oligospermia are effectively improved: (1) the ratio of sperm forward motility; (2) the ratio of sperm forward motility after semen freezing and resuscitation; (3) the sperm DNA fragmentation rate after semen freezing and resuscitation; (4) the sperm acrosome enzyme content after semen freezing and resuscitation; in summary, pyridoxamine can be added in the sperm operation liquid or the semen freezing liquid as a natural semen protective agent, the sperm selection treatment and the freezing and resuscitation effect of patients with oligospermia are obviously improved, and the application is suitable for application of patients with oligospermia in assisted reproductive treatment and fertility preservation.

The present invention relates to a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation. According to a first aspect of the present invention, there is provided a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation, the method comprising:providing an image of the sperm cell, under brightfield and / or phase contrast with a total magnification of 400× to 1000×;evaluating the image of the sperm cell to identify and / or measure a pre-selected biomarker; andapproximating the output of the pre-selected chemical assay of sperm DNA fragmentation of the sperm cell by subjecting the identified and / or measured biomarker to a first machine learning analysis.

The invention relates to an improved method for analysing sperm DNA structure, by means of which it is possible to reduce the time required to process samples by 80%, optimising every stage of the process. This effect is achieved through a synergetic improvement in each step of the process. The formation of microgels is accelerated with cold metal plates (2–8°C) and using 0.8%-1.5% agarose. DNA denaturation is carried out using HCl (100 mM). The deproteinisation solution contains DTT (0.22 M), NaCl (2.75 M), detergents of the alcohol ethoxylate (0.044 M) and SDS (0.0014 M) type. Fixation is carried out using 96% ethanol at a low temperature. The staining process uses a panoptic dye, improving the quality of the images. The invention not only speeds up the analysis by reducing the time required by 80%, but also enables faster and more accurate diagnoses, thanks to the high quality of the images obtained.

The invention provides an application of pyridoxamine in sperm optimization and / or freezing recovery operation of asthenospermia, pyridoxamine is added in sperm operation liquid or sperm freezing liquid, and the following shortages of asthenospermia patients are effectively improved: (1) the ratio of sperm forward motility sperms; (2) determining the ratio of forward motility sperms after the semen is frozen and resuscitated; (3) determining the sperm DNA fragment rate after the sperm is frozen and resuscitated; (4) determining the content of sperm acrosomal enzyme after the sperm is frozen and resuscitated; in conclusion, the pyridoxamine can be added into sperm operating fluid or sperm refrigerating fluid as a natural sperm protective agent, the sperm optimization treatment and freezing resuscitation effects of the asthenospermia patient are obviously improved, and the pyridoxamine is suitable for being applied to assisted reproductive therapy and fertility preservation of the asthenospermia patient.

The application discloses a label-free sperm quality evaluation method, system and device. The application is based on a high-throughput multi-dimensional flow cytometry imaging system, obtains intensity, phase, bright field and dark field images of sperm cells, establishes a massive sperm cell image database, and constructs, trains and optimizes a sperm cell multi-dimensional image analysis model based on a multi-model fusion deep learning network. On this basis, the trained sperm cell multi-dimensional image analysis network model is used to analyze sperm cell images obtained by the multi-dimensional flow cytometry imaging system in the detection of a semen sample, analyze sperm structure and function parameters such as the proportion of each morphological category of sperm in the semen sample and the sperm DNA fragmentation rate, and realize high-speed, high-precision and label-free sperm quality evaluation.

The present invention relates to a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation. According to a first aspect of the present invention, there is provided a method for analysing DNA fragmentation in a sperm cell by approximating the output of a pre-selected chemical assay of sperm DNA fragmentation, the method comprising:providing an image of the sperm cell, under brightfield and / or phase contrast with a total magnification of 400× to 1000×;evaluating the image of the sperm cell to identify and / or measure a pre-selected biomarker; andapproximating the output of the pre-selected chemical assay of sperm DNA fragmentation of the sperm cell by subjecting the identified and / or measured biomarker to a first machine learning analysis.

The invention relates to the technical field of image processing, and provides a sperm DNA fragment recognition method and device, and the method comprises the steps: recognizing a sperm image, so as to obtain the contour information of a plurality of candidate sperms from the sperm image, and the contour information of the candidate sperms comprises a head contour, an overall contour and a halo contour; according to the head contour center point of each candidate sperm, determining a target overall contour from the overall contours of the plurality of candidate sperms to construct a target overall contour set; determining a target halo contour from the halo contours of the plurality of candidate sperms according to a head contour center point corresponding to the target overall contour in the target overall contour set so as to construct a target halo contour set; and according to each target halo contour in the target halo contour set, identifying DNA fragments of the candidate sperms. According to the method, the sperm head and tail features are combined for recognition, the sperms overlapped in the halo area can be accurately recognized, and the recognition accuracy and efficiency of sperm DNA fragments are improved.

This invention, entitled "A Fertilization Culture Medium, Its Preparation Method, and Its Application," belongs to the field of assisted reproductive technology. The technical problem it aims to solve is that existing technologies, due to the generation of sperm DNA fragments during the culture process, lead to a decrease in overall sperm quality, limiting fertilization rate and blastocyst formation rate. This invention proposes a fertilization culture medium that can improve overall sperm quality and thus increase fertilization rate. The key technical points are: a fertilization culture medium comprising the following components: sodium chloride, potassium chloride, alkali metal dihydrogen phosphate, calcium chloride, magnesium sulfate, sodium bicarbonate, sodium lactate, sodium pyruvate, glucose, fructose, disodium edetate, gentamicin, human serum albumin, alanylglutamine, taurine, non-essential amino acids, and testosterone.

The present invention relates to a method and a kit for detecting sperm DNA fragmentation. The present invention discloses a method for detecting DNA fragmentation of sperm in a semen sample, comprising the steps of: embedding the semen sample in a gel having a pore size of 2 nm or more and less than 60 nm to obtain a sperm-embedded gel; performing a lysis treatment on the sperm-embedded gel with a lysis solution to lyse the nuclear proteins of the sperm, wherein the lysis solution comprises urea and sodium dodecyl sulfate; performing a DNA staining on the lysed gel; and observing whether a halo is formed at the periphery of the head of each sperm on the stained gel, wherein the presence of halo formation is indicative of the presence of sperm DNA fragmentation. The present invention also discloses a kit for performing the method.