754 results about "Spermatid" patented technology

This disclosure relates to homodimeric antibodies for use in treating cancer generally and to both homodimeric antibodies that bind human sperm protein 17 (Sp17) and homodimeric antibody immunoconjugates specifically. Such homodimeric antibodies display improved properties relative to monomeric antibodies, for example, because they can crosslink cells, improve cellular uptake, and / or carry greater payloads.

This disclosure relates to dimeric immunoconjugates for use in treating cancer generally and to dimeric immunoconjugates that bind human sperm protein 17 (Sp17) specifically. A dimeric immunoconjugate of this disclosure generally comprises a first monomeric antibody that carries a first payload and a second monomeric antibody that carries a second payload, wherein the first payload and the second payload are different. Such dimeric immunoconjugates advantageously allow the simultaneous delivery of two chemotherapeutics to a cancer cell, which allows for synergistic antineoplastic activity.

The invention provides application of a protein deacetylase inhibitor NAM in ultralow-temperature cryopreservation of poultry semen, and belongs to the technical field of poultry breed conservation. The protein deacetylase inhibitor NAM is used as a cryoprotectant or is combined with other cryoprotectants for use, and the protein deacetylase inhibitor NAM is used as an effective component, so that the ultralow-temperature cryopreservation efficiency of poultry can be improved. Wherein the protein deacetylase inhibitor NAM can inhibit sperm cell deacetylation modification so as to maintain a high acetylation modification level, improve the ability of sperms to respond to ultralow temperature freezing stimulation and improve the fertilization ability of frozen-remelted sperms.

The invention discloses an HNRNPA2B1 gene-based bovine sperm malformation rate related SNP molecular marker and application thereof, and belongs to the technical field of genetic molecular biology, the number of the SNP molecular markers is two, SNP1 is at the position of a bovine genome chr4: 69733358, the polymorphism is C or A, SNP2 is at the position of a bovine genome chr4: 69733387, and the polymorphism is T or G. According to the two SNP loci provided by the invention, the dominant genotypes of g.-1713A > C and g.-1684G > T are AA and GG respectively, and the sperm collection quantity and the sperm malformation rate of the two SNP loci have better performance. By detecting the polymorphism of the two SNP loci, the motility evaluation of the bull sperms can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.

The invention relates to the technical field of multi-modal fusion, in particular to a sperm quality assessment method and system based on multi-modal fusion, and the method comprises the following steps: obtaining image and parameter data to generate a space-time alignment set, extracting trajectory calculation linearity, analyzing parameter fluctuation to mark an abnormal time window, extracting an image assessment deformity rate to generate a feature matrix, and grading to generate a multi-modal sperm quality evaluation result. According to the method, linear calculation is introduced in the trajectory path extraction process, spatial distribution thermodynamic mapping is combined, the dynamic characteristics of local sperm populations are dominated, an abnormal time window is constructed based on the parameter value change rate and a set threshold value, and motion consistency indexes are combined, so that the dynamic characteristics of the local sperm populations are optimized. The explanation depth of sperm head malformation data in quality judgment is enhanced, the dimension space of sperm quality description is effectively expanded, the consistency ratio between vitality and morphology is introduced, track continuous characteristics are combined, a hierarchical grading system is established, and the accuracy of sperm quality evaluation results is ensured.

The invention relates to a sperm cell analysis and diagnosis system based on a multi-modal large language model, which comprises a multi-modal data co-processing unit, a cross-modal semantic alignment module, a dynamic diagnosis decision engine and a self-adaptive evolution system, the four-dimensional data processing module is used for synchronously processing microscopic images, motion trail videos, biochemical detection data and four-dimensional input data of medical record texts and comprises a feature selector based on a gating attention mechanism. According to the sperm cell analysis and diagnosis system based on the multi-modal large language model, quantitative analysis of sperm movement chaos features is realized for the first time, a nonlinear dynamic evaluation standard is established, a cross-modal knowledge distillation and meta-learning migration framework is developed, a data annotation dependence bottleneck is broken through, and an interpretable clinical decision support system is constructed; dynamic updating and probabilistic suggestion of diagnosis rules are achieved, semantic analysis of single-cell multi-omics data is achieved, and molecular mechanism research results are converted into clinically available knowledge.

The invention discloses a pig sperm specific antibody. The coding sequence of the antibody is shown as any one of SEQ ID No: 1-3. According to the method, the nano antibody library is prepared by immunizing alpaca with whole pig sperms, and then the antibody capable of being specifically combined with the X or Y sperms can be screened out through the library, so that sorting of the X and Y sperms is realized. The screened antibody is a nano antibody, the size of the nano antibody is only 10% of that of a traditional antibody, the structure is simpler, the advantages of high stability, strong specificity and low immunogenicity are achieved, large-scale expression of the antibody can be achieved, the production cost of the antibody is greatly reduced, and industrial application is facilitated.

The invention provides a feed domesticated siniperca chuatsi sperm low-temperature preservation and ultralow-temperature preservation method, which comprises the following steps: firstly, providing a siniperca chuatsi sperm low-temperature preservation diluent which comprises Hanks liquid, sodium chloride, soya bean lecithin, vitamin E, curcumin, melatonin and SOD (superoxide dismutase); and adding an anti-freezing protective agent into the low-temperature preservation diluent to obtain the ultralow-temperature preservation anti-freezing liquid for the siniperca chuatsi sperms. The siniperca chuatsi semen and the low-temperature preservation diluent are uniformly mixed, so that low-temperature preservation can be realized; the siniperca chuatsi semen is uniformly mixed with the cryopreservation anti-freezing liquid, so that cryopreservation can be realized. The siniperca chuatsi sperm low-temperature preservation diluent is preferably selected, low-temperature preservation of the siniperca chuatsi sperms can be achieved, ultralow-temperature preservation can be conducted after an anti-freezing protective agent is added, the persistence of the preservation time of the siniperca chuatsi sperms can be guaranteed, the protection effect on the sperms is good, and the activation rate and activity of the unfrozen sperms are high.

The invention discloses a sperm real-time tracking and segmentation method based on a multi-task cascade analysis network, and the method comprises the following steps: S1, obtaining a sperm bright field image under a microscope, and carrying out the preprocessing of the sperm bright field image; s2, a detection module is adopted to carry out sperm head positioning detection on the preprocessed image, and rapid detection of a high-resolution sperm image is realized through combination of the improved detection module and a self-developed attention mixed feature down-sampler; s3, a sperm head detection result is cut and input to the lightweight real-time segmentation network for real-time segmentation, the model parameter quantity is significantly reduced, and the segmentation precision is improved; and S4, outputting a real-time tracking result to the sperm target in the continuous frames by using a tracking algorithm, and measuring and calculating the activity parameter of the target sperm. Through joint optimization of detection, segmentation and data enhancement modules, an efficient and robust automatic solution is provided for the assisted reproduction technology, and the method can be widely applied to sperm real-time analysis and screening in the assisted reproduction technology.

The invention relates to the technical field of microorganisms, and provides Witzia ciliaris KY009 (preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 33480) and Clostridium butyricum KY002 (preservation number is CGMCC NO. 33478), and the two strains are separated from an excrement sample. Experiments prove that the two strains can obviously improve the sperm quality and quantity, regulate the sex hormone level, improve the intestinal inflammation symptom and regulate the expression of related inflammatory factors. The invention also provides a composition containing the two strains, wherein the composition comprises a food composition, a pharmaceutical composition, a health-care product composition, a functional microbial agent and the like. The compositions are useful for improving reproductive health, modulating sex hormone levels, treating intestinal inflammation and promoting mucosal repair, and alleviating metabolic and immune related diseases. The strain and the composition provided by the invention have wide application prospects in the fields of medicines, foods and health-care products.

The invention provides application of a sperm tRNA (transfer ribonucleic acid) derived fragment 5 'tRF-Glu-CTC in prediction of development potential of assisted reproductive embryos. The expression of a tRNA derived fragment 5 'tRF-Glu-CTC in a sperm sample is detected, the abnormal expression of the sperm specific tRNA derived fragment 5' tRF-Glu-CTC in a DFI increase patient is verified, and the analysis on the correlation with clinical data and reproductive outcome of the patient prompts that the 5 'tRF-Glu-CTC molecular marker has significant correlation with the reproductive outcome, so that the 5' tRF-Glu-CTC molecular marker can be used for detecting the expression of the sperm specific tRNA derived fragment 5 'tRF-Glu-CTC in the DFI increase patient. The embryonic development potential can be effectively predicted, and a biomarker is provided for assisted reproduction treatment. In different experimental groups, the 5 'tRF-Glu-CTC can significantly improve the prediction precision, and shows good prediction performance in different clinical data. The detection method based on the fragment is expected to become a new tool for evaluating the assisted reproduction curative effect, the reproduction treatment scheme is further optimized, and the success rate is improved.

The present disclosure provides a sperm extractor, the sperm extractor may be a stimulation device, the sperm extractor including: a first massage member and a first driving mechanism, the first massage member including a first end, a second end opposite to the first end, and a lateral wall between the first end and the second end, the lateral wall surrounding and defining a first inserting space, the lateral wall including a first massage portion capable of massaging a glans of the penis received in the first inserting space; the first massage portion is driven to move by a motion of the connecting portion, the first massage portion is entirely deviating toward a first direction intersected with the inserting direction at a first moment and entirely deviating toward a second direction intersected with the inserting direction at a second moment, the first direction is different to the second direction.

The invention relates to the technical field of genetic relationship identification, in particular to a construction method, an identification method and application of a primary genetic relationship identification model based on ultralow deep sequencing. According to the identification model obtained by using the construction method of the genetic relationship identification model provided by the invention, the genetic relationship can be identified by adopting ultra-low depth sequencing (0.01 *), the genetic relationship can be determined according to the heterozygosity index between samples to be identified, the prediction accuracy is up to 99.9%, and the construction method is a first-grade genetic relationship detection technology with low cost and high accuracy; the method has the advantages of simple and convenient scheme, high accuracy, high credibility, high repeatability and high economic benefit, has multiple application scenes, and can advance an embryo paternity test window from antenatal / postpartum to before embryo implantation, thereby avoiding physical, mental and economic losses of a patient; ovum / sperm fertilization errors can be found in advance, non-parent embryo transplantation is avoided, and loss caused by embryo wrong transplantation is prevented.

The invention discloses a traditional Chinese medicine compound oral preparation for treating male oligoasthenospermia, and belongs to the technical field of traditional Chinese medicine preparations. The rhizoma polygonati processed with wine, the prepared rehmannia root and the like are used for nourishing the liver and kidney to consolidate congenital, the codonopsis pilosula, the astragalus membranaceus and the like are used for invigorating spleen and replenishing qi to complement postnatal, the fried cowherb seed is used for activating blood circulation, and the salted semen plantaginis is used for promoting diuresis, so that the problems of insufficient spermatogenesis and low vitality caused by deficiency of the spleen and the kidney are fundamentally solved. Experiments and clinical researches show that the preparation can significantly improve the sperm quantity, activity and activity rate of oligoasthenospermia model mice and patients, the clinical total effective rate reaches 90%, the pregnancy rate reaches 53.3%, and the curative effect is superior to that of Wuzi Yanzong pills in a control group. The action mechanism covers regulation of blood lipid metabolism, improvement of hypothalamus-pituitary-gonad axis functions, repair of testicular seminiferous tubule structural damage and reduction of lipid deposition, and meanwhile, oxidative stress and inflammatory injury are relieved and spermatogenic cell functions are protected by regulating and controlling a PPAR signal channel and inhibiting molecular pathways such as Cga / Sting mediated cell apoptosis.

The invention discloses a spermatogenesis disorder sperm image recognition system based on deep learning, and relates to the technical field of sperm image recognition. The image recognition management center is in communication connection with an image acquisition module, a sperm positioning module, a feature extraction module, a motion feature analysis module, a sperm form classification module, a sperm state comprehensive recognition module and a result display feedback module, and all the modules are in electric signal connection. According to the method, morphology and motion features are learned and extracted from a large amount of sperm image data through a deep learning technology, the accuracy of sperm classification is improved, the time and errors of manual operation are remarkably reduced, meanwhile, fine differences of sperms can be accurately recognized, the diagnosis efficiency is greatly improved, and the method is suitable for popularization and application. Therefore, a doctor can obtain a more accurate diagnosis result in a shorter time, and a more timely treatment suggestion is provided for a patient.

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention discloses an anti-freezing solution for improving the survival rate of sperms of Chinese prawns and a cryopreservation method of the anti-freezing solution, and belongs to the technical field of low-temperature biology and reproductive science. The invention provides an anti-freezing solution for cryopreservation of sperms of Chinese prawns, which comprises a basic solution and an anti-freezing agent, and the anti-freezing agent is dimethyl sulfoxide with the volume fraction of 10% or glycerol with the volume fraction of 10%; the basic liquid contains 23.69 g of sodium chloride, 1.1 g of potassium chloride, 14.8 g of magnesium chloride hexahydrate, 4.3 g of sodium sulfate, 0.23 g of sodium bicarbonate, 1.12 g of calcium chloride and distilled water with the constant volume of 1000 mL. According to the cryopreservation method provided by the invention, 10% dimethyl sulfoxide or 10% glycerol is adopted as an antifreeze agent, the sperm density is controlled to be 1 * 10 < 7 > / mL, and step-by-step cooling is carried out, so that the survival rate of unfrozen sperms reaches 90% or above. The long-term preservation effect of the Chinese prawn sperms is remarkably improved, and the method is suitable for germplasm resource protection and efficient breeding.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention belongs to the field of fish germplasm improvement and innovation, and discloses an application of river perch sperms in inducing gynogenesis of largemouth bass, which comprises the following steps: obtaining mature largemouth bass ova and river perch sperms through artificial spawning induction; the method comprises the following steps: collecting river perch semen, diluting, oscillating, irradiating and inactivating with an ultraviolet lamp, mixing with mature largemouth bass ova, inseminating, and hatching fertilized ova to obtain gynogenesis largemouth bass. According to the method, the sperms of the river perches are used for stimulating gynogenesis of the micropterus salmoides for the first time, the gynogenesis micropterus salmoides is successfully obtained, and the heterologous sperms in the far geographic space are used as the stimulation source of the artificial gynogenesis ovum for the first time. The method not only provides an innovative germplasm resource basis in the aspect of improving germplasm of the micropterus salmoides, but also has important significance in the research aspects of fish genetic breeding and'micro-hybridization among fish species in far geographic space '.

The present disclosure generally relates to micromanipulation devices and methods for the manipulation of mammalian sub-cellular structures. Uses include intra cytoplasmic sperm injection procedures, embryo biopsy procedures, trophectoderm biopsy procedures, mitochondrial transfer procedures, pronuclear transfer procedures, maternal spindle transfer procedures, cumulus cell biopsy procedures and other sub-cellular manipulation procedures. Devices comprise a cellular manipulation dish further comprising multiple sub- cellular manipulation units having a microwell and a cell deposit well wherein the cell deposit opening is disposed adjacent to the microwell opening.

To provide a sperm selection assisting device, a projection device, a microscope system, etc. that efficiently assist in selecting good sperm suitable for intracytoplasmic sperm injection from sperm in a specimen. [Solution] A sperm sorting assistance device comprising: an acquisition unit that acquires an observation image of sperm; a reception unit that accepts a sperm evaluation method; an evaluation unit that evaluates the sperm contained in the acquired observation image based on the evaluation method; and a display control unit that controls a projection device provided in the microscope to display, on an observation image presentation unit in the microscope, a composite image in which the evaluation results by the evaluation unit for at least one sperm in the observation image are displayed corresponding to each sperm, and which further displays the evaluation method.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for influencing the linear motion / far-end mass droplet character of a pig sperm. The SNP molecular marker is Ggt (Glutathione Ggt) which is positioned at the 31908556 bp position of the 10th chromosome of a pig genome Ensembl Sscrofa11.1 version; a mutation. By verifying the influence effect of the molecular marker on linear motion and far-end mass droplet characters of pig sperms, an efficient and accurate molecular marker assisted breeding technology is finally established, and the molecular marker assisted breeding technology is applied to genetic improvement of boars for improving the quality of the boar sperms, so that the reproductive performance of offspring pigs is improved, the economic profit of enterprises is increased, and the core competitiveness is increased. By optimizing the dominant allele A of the SNP, the frequency of the dominant allele A can be increased generation by generation, the semen quality of the boars can be improved, excellent boars with the two characters can be bred cooperatively, the genetic improvement progress of the boars can be accelerated, and the economic benefit of boar breeding can be effectively improved.

The present invention belongs to the technical field of semen cryopreservation, and more specifically, relates to sheep semen freezing solution and semen preservation method. The sheep semen freezing solution components of the present invention include Tris, citric acid, fructose, lactose, trehalose, penicillin, streptomycin, soy lecithin, nobiletin, glycerol and water. By using a specific content of nobiletin as an effective antioxidant component of a sheep sperm cryopreservative, it plays an antioxidant role when the sperm is frozen, making the sperm more stable during the cryopreservation process. After adding nobiletin, the vitality and motility of sheep sperm after freezing and thawing can be significantly improved. It effectively reduces the oxidative stress damage of sperm during the freezing process and improves the antioxidant capacity of sperm.

The invention provides a functional peptide with a yang tonifying effect, a seal penis peptide and a preparation method and application of the functional peptide and the seal penis peptide. The functional peptide comprises a peptide fragment pER and can promote testicular interstitial cells to secrete testosterone. Testosterone is steroid hormone and can be secreted by testicular interstitial cells through enzymatic reaction. The low testosterone level in serum can cause male testicular atrophy and low sperm survival rate, so that the male reproductive function is influenced, and the phenomena of poor sexual desire, premature ejaculation, erectile dysfunction and the like can also be caused. In addition, the testosterone also has the effects of maintaining muscle strength and quality, maintaining bone density and strength, refreshing, improving physical ability and the like. Therefore, the functional peptide provided by the invention has the effect of promoting the secretion of testosterone, which means that the functional peptide not only can effectively improve the male sexual function, but also can alleviate the damage of male sexual dysfunction to the body and spirit.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

The invention relates to an artificial cross breeding method of Jinlong hybrid spot new germplasm, which belongs to the technical field of aquatic product distant cross breeding, and comprises the following steps: (1) parent breeding; (2) collecting and storing sperms of epinephelus lanceolatus; (3) carrying out reproductive regulation and ovum acquisition on the epinephelus coioides; (4) artificial preparation and incubation of hybrid fertilized eggs; according to the method, the artificial cross breeding of the female epinephelus coioides and the male epinephelus lanceolatus is realized for the first time, and the new germplasm Jinlong hybrid spots of the epinephelus coioides, which grow fast and are high in survival rate, are cultivated. The body weight of the Jinlong hybrid Epinephelus coioides of 3 months old is increased by 46.45% compared with that of pure Epinephelus coioides; and the survival rate of the new germplasm is increased by 62.50% compared with that of the epinephelus coioides. The industrial problems that artificial spawning of large groupers is difficult, the survival rate of fries is low, and fast-growing new germplasm lacks are effectively solved.

Provided in the present utility model is a sperm extractor with double folding handles, including: a housing; the double folding handles mounted on the housing by mounting brackets; and an inner container mounted inside the housing, where the double folding handles include a left handle and a right handle that are disposed on a left side and a right side of the housing respectively; the mounting brackets include a first mounting bracket and a second mounting bracket that are disposed on an upper side and a lower side of the housing respectively.

The invention provides a breeding method of fast-growing high-quality Dongzhihuo. Comprising the following steps: identifying the genotype of an SNP (Single Nucleotide Polymorphism) locus which is located at the 79th site of a gene sequence as shown in SEQ ID NO.1, and if the genotype of the locus is TT type, determining that the locus is a candidate parent; selecting candidate parents with uniform and robust body types as parent fishes; carrying out spawning induction on the male fish and the female fish of the Dongstellate star; collecting ova and sperms for artificial insemination; uniformly putting the fertilized eggs into an indoor nursery pond for hatching and breeding. The euryale ferox bred through the method is high in growth and development speed, high in muscle fat content, high in crude protein content, high in flavor amino acid content, delicious in taste, more popular with consumers and good in market prospect.

The invention relates to the crossing field of computer vision and biomedical engineering, and discloses a method and a system for sperm kinetic analysis, comprising the following steps: concurrently performing environmental analysis and target detection on a microscope image sequence, generating a mask for identifying the position of an interferent and an index for quantifying scene complexity, positioning sperms by the latter; the core is a self-adaptive trajectory tracking module which receives the two paths of information and performs tracking based on a dual feedback mechanism: on one hand, the correlation cost of a detection result in an interferent mask area is set as a maximum value, so that wrong matching is avoided; and on the other hand, the internal parameters of the tracking model are dynamically adjusted according to the scene complexity index. According to the method, dynamic parameters such as VCL, VSL, ALH and BCF are accurately calculated by generating the robust motion trail, a comprehensive report containing a quantitative result and analysis quality control evaluation is finally output, and the accuracy and credibility of an analysis result are remarkably improved.

A massage device characterized by comprising: a housing, the housing having an opening formed through a side of the housing for inserting the part to be massaged; a first drive mechanism housed in the housing; and a receiving portion having one end fixedly connected to the inner wall of the housing and communicating with the opening, the receiving portion extending from one end along the insertion direction of the part to be massaged and the receiving portion being connected to the first drive mechanism, and the receiving portion reciprocating relative to the housing under the drive of the first drive mechanism to massage the part to be massaged.