614 results about "Spermatid" patented technology

This disclosure relates to dimeric immunoconjugates for use in treating cancer generally and to dimeric immunoconjugates that bind human sperm protein 17 (Sp17) specifically. A dimeric immunoconjugate of this disclosure generally comprises a first monomeric antibody that carries a first payload and a second monomeric antibody that carries a second payload, wherein the first payload and the second payload are different. Such dimeric immunoconjugates advantageously allow the simultaneous delivery of two chemotherapeutics to a cancer cell, which allows for synergistic antineoplastic activity.

The invention relates to the technical field of multi-modal fusion, in particular to a sperm quality assessment method and system based on multi-modal fusion, and the method comprises the following steps: obtaining image and parameter data to generate a space-time alignment set, extracting trajectory calculation linearity, analyzing parameter fluctuation to mark an abnormal time window, extracting an image assessment deformity rate to generate a feature matrix, and grading to generate a multi-modal sperm quality evaluation result. According to the method, linear calculation is introduced in the trajectory path extraction process, spatial distribution thermodynamic mapping is combined, the dynamic characteristics of local sperm populations are dominated, an abnormal time window is constructed based on the parameter value change rate and a set threshold value, and motion consistency indexes are combined, so that the dynamic characteristics of the local sperm populations are optimized. The explanation depth of sperm head malformation data in quality judgment is enhanced, the dimension space of sperm quality description is effectively expanded, the consistency ratio between vitality and morphology is introduced, track continuous characteristics are combined, a hierarchical grading system is established, and the accuracy of sperm quality evaluation results is ensured.

The invention relates to a sperm cell analysis and diagnosis system based on a multi-modal large language model, which comprises a multi-modal data co-processing unit, a cross-modal semantic alignment module, a dynamic diagnosis decision engine and a self-adaptive evolution system, the four-dimensional data processing module is used for synchronously processing microscopic images, motion trail videos, biochemical detection data and four-dimensional input data of medical record texts and comprises a feature selector based on a gating attention mechanism. According to the sperm cell analysis and diagnosis system based on the multi-modal large language model, quantitative analysis of sperm movement chaos features is realized for the first time, a nonlinear dynamic evaluation standard is established, a cross-modal knowledge distillation and meta-learning migration framework is developed, a data annotation dependence bottleneck is broken through, and an interpretable clinical decision support system is constructed; dynamic updating and probabilistic suggestion of diagnosis rules are achieved, semantic analysis of single-cell multi-omics data is achieved, and molecular mechanism research results are converted into clinically available knowledge.

The invention discloses a sperm real-time tracking and segmentation method based on a multi-task cascade analysis network, and the method comprises the following steps: S1, obtaining a sperm bright field image under a microscope, and carrying out the preprocessing of the sperm bright field image; s2, a detection module is adopted to carry out sperm head positioning detection on the preprocessed image, and rapid detection of a high-resolution sperm image is realized through combination of the improved detection module and a self-developed attention mixed feature down-sampler; s3, a sperm head detection result is cut and input to the lightweight real-time segmentation network for real-time segmentation, the model parameter quantity is significantly reduced, and the segmentation precision is improved; and S4, outputting a real-time tracking result to the sperm target in the continuous frames by using a tracking algorithm, and measuring and calculating the activity parameter of the target sperm. Through joint optimization of detection, segmentation and data enhancement modules, an efficient and robust automatic solution is provided for the assisted reproduction technology, and the method can be widely applied to sperm real-time analysis and screening in the assisted reproduction technology.

The invention relates to the technical field of microorganisms, and provides Witzia ciliaris KY009 (preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 33480) and Clostridium butyricum KY002 (preservation number is CGMCC NO. 33478), and the two strains are separated from an excrement sample. Experiments prove that the two strains can obviously improve the sperm quality and quantity, regulate the sex hormone level, improve the intestinal inflammation symptom and regulate the expression of related inflammatory factors. The invention also provides a composition containing the two strains, wherein the composition comprises a food composition, a pharmaceutical composition, a health-care product composition, a functional microbial agent and the like. The compositions are useful for improving reproductive health, modulating sex hormone levels, treating intestinal inflammation and promoting mucosal repair, and alleviating metabolic and immune related diseases. The strain and the composition provided by the invention have wide application prospects in the fields of medicines, foods and health-care products.

The invention provides application of a sperm tRNA (transfer ribonucleic acid) derived fragment 5 'tRF-Glu-CTC in prediction of development potential of assisted reproductive embryos. The expression of a tRNA derived fragment 5 'tRF-Glu-CTC in a sperm sample is detected, the abnormal expression of the sperm specific tRNA derived fragment 5' tRF-Glu-CTC in a DFI increase patient is verified, and the analysis on the correlation with clinical data and reproductive outcome of the patient prompts that the 5 'tRF-Glu-CTC molecular marker has significant correlation with the reproductive outcome, so that the 5' tRF-Glu-CTC molecular marker can be used for detecting the expression of the sperm specific tRNA derived fragment 5 'tRF-Glu-CTC in the DFI increase patient. The embryonic development potential can be effectively predicted, and a biomarker is provided for assisted reproduction treatment. In different experimental groups, the 5 'tRF-Glu-CTC can significantly improve the prediction precision, and shows good prediction performance in different clinical data. The detection method based on the fragment is expected to become a new tool for evaluating the assisted reproduction curative effect, the reproduction treatment scheme is further optimized, and the success rate is improved.

The present disclosure provides a sperm extractor, the sperm extractor may be a stimulation device, the sperm extractor including: a first massage member and a first driving mechanism, the first massage member including a first end, a second end opposite to the first end, and a lateral wall between the first end and the second end, the lateral wall surrounding and defining a first inserting space, the lateral wall including a first massage portion capable of massaging a glans of the penis received in the first inserting space; the first massage portion is driven to move by a motion of the connecting portion, the first massage portion is entirely deviating toward a first direction intersected with the inserting direction at a first moment and entirely deviating toward a second direction intersected with the inserting direction at a second moment, the first direction is different to the second direction.

The invention discloses a traditional Chinese medicine compound oral preparation for treating male oligoasthenospermia, and belongs to the technical field of traditional Chinese medicine preparations. The rhizoma polygonati processed with wine, the prepared rehmannia root and the like are used for nourishing the liver and kidney to consolidate congenital, the codonopsis pilosula, the astragalus membranaceus and the like are used for invigorating spleen and replenishing qi to complement postnatal, the fried cowherb seed is used for activating blood circulation, and the salted semen plantaginis is used for promoting diuresis, so that the problems of insufficient spermatogenesis and low vitality caused by deficiency of the spleen and the kidney are fundamentally solved. Experiments and clinical researches show that the preparation can significantly improve the sperm quantity, activity and activity rate of oligoasthenospermia model mice and patients, the clinical total effective rate reaches 90%, the pregnancy rate reaches 53.3%, and the curative effect is superior to that of Wuzi Yanzong pills in a control group. The action mechanism covers regulation of blood lipid metabolism, improvement of hypothalamus-pituitary-gonad axis functions, repair of testicular seminiferous tubule structural damage and reduction of lipid deposition, and meanwhile, oxidative stress and inflammatory injury are relieved and spermatogenic cell functions are protected by regulating and controlling a PPAR signal channel and inhibiting molecular pathways such as Cga / Sting mediated cell apoptosis.

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention discloses an anti-freezing solution for improving the survival rate of sperms of Chinese prawns and a cryopreservation method of the anti-freezing solution, and belongs to the technical field of low-temperature biology and reproductive science. The invention provides an anti-freezing solution for cryopreservation of sperms of Chinese prawns, which comprises a basic solution and an anti-freezing agent, and the anti-freezing agent is dimethyl sulfoxide with the volume fraction of 10% or glycerol with the volume fraction of 10%; the basic liquid contains 23.69 g of sodium chloride, 1.1 g of potassium chloride, 14.8 g of magnesium chloride hexahydrate, 4.3 g of sodium sulfate, 0.23 g of sodium bicarbonate, 1.12 g of calcium chloride and distilled water with the constant volume of 1000 mL. According to the cryopreservation method provided by the invention, 10% dimethyl sulfoxide or 10% glycerol is adopted as an antifreeze agent, the sperm density is controlled to be 1 * 10 < 7 > / mL, and step-by-step cooling is carried out, so that the survival rate of unfrozen sperms reaches 90% or above. The long-term preservation effect of the Chinese prawn sperms is remarkably improved, and the method is suitable for germplasm resource protection and efficient breeding.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The present disclosure generally relates to micromanipulation devices and methods for the manipulation of mammalian sub-cellular structures. Uses include intra cytoplasmic sperm injection procedures, embryo biopsy procedures, trophectoderm biopsy procedures, mitochondrial transfer procedures, pronuclear transfer procedures, maternal spindle transfer procedures, cumulus cell biopsy procedures and other sub-cellular manipulation procedures. Devices comprise a cellular manipulation dish further comprising multiple sub- cellular manipulation units having a microwell and a cell deposit well wherein the cell deposit opening is disposed adjacent to the microwell opening.

To provide a sperm selection assisting device, a projection device, a microscope system, etc. that efficiently assist in selecting good sperm suitable for intracytoplasmic sperm injection from sperm in a specimen. [Solution] A sperm sorting assistance device comprising: an acquisition unit that acquires an observation image of sperm; a reception unit that accepts a sperm evaluation method; an evaluation unit that evaluates the sperm contained in the acquired observation image based on the evaluation method; and a display control unit that controls a projection device provided in the microscope to display, on an observation image presentation unit in the microscope, a composite image in which the evaluation results by the evaluation unit for at least one sperm in the observation image are displayed corresponding to each sperm, and which further displays the evaluation method.

The present invention belongs to the technical field of semen cryopreservation, and more specifically, relates to sheep semen freezing solution and semen preservation method. The sheep semen freezing solution components of the present invention include Tris, citric acid, fructose, lactose, trehalose, penicillin, streptomycin, soy lecithin, nobiletin, glycerol and water. By using a specific content of nobiletin as an effective antioxidant component of a sheep sperm cryopreservative, it plays an antioxidant role when the sperm is frozen, making the sperm more stable during the cryopreservation process. After adding nobiletin, the vitality and motility of sheep sperm after freezing and thawing can be significantly improved. It effectively reduces the oxidative stress damage of sperm during the freezing process and improves the antioxidant capacity of sperm.

The invention relates to the technical field of biology, in particular to application of rosa roxburghii tratt active extract in preparation of a medicine for treating dyszoospermia. The extract provided by the invention is prepared from roxburgh rose fruits or roxburgh rose pomace through extraction and enrichment, the raw material resources are rich, the price is low, and the toxic and side effects are low. Animal experiments show that the roxburgh rose active extract has the activity of improving dyszoospermia, and the roxburgh rose active extract can obviously increase the sperm concentration and motility rate of mice with dyszoospermia, reduce the malformation rate and obviously increase the thickness of spermatogenic epithelium, the number of supporting cells and the number of interstitial cells of testicular tissues of the mice with dyszoospermia. WB detection analysis shows that the action way of improving the dyszoospermia by the roxburgh rose active extract is to inhibit activation of an oxidative stress Keap1 / Nrf2 signal channel. The rosa roxburghii tratt active extract has a new function of improving dyszoospermia and can be applied as a medicine for improving dyszoospermia.

The invention relates to an artificial cross breeding method of Jinlong hybrid spot new germplasm, which belongs to the technical field of aquatic product distant cross breeding, and comprises the following steps: (1) parent breeding; (2) collecting and storing sperms of epinephelus lanceolatus; (3) carrying out reproductive regulation and ovum acquisition on the epinephelus coioides; (4) artificial preparation and incubation of hybrid fertilized eggs; according to the method, the artificial cross breeding of the female epinephelus coioides and the male epinephelus lanceolatus is realized for the first time, and the new germplasm Jinlong hybrid spots of the epinephelus coioides, which grow fast and are high in survival rate, are cultivated. The body weight of the Jinlong hybrid Epinephelus coioides of 3 months old is increased by 46.45% compared with that of pure Epinephelus coioides; and the survival rate of the new germplasm is increased by 62.50% compared with that of the epinephelus coioides. The industrial problems that artificial spawning of large groupers is difficult, the survival rate of fries is low, and fast-growing new germplasm lacks are effectively solved.

The invention relates to the crossing field of computer vision and biomedical engineering, and discloses a method and a system for sperm kinetic analysis, comprising the following steps: concurrently performing environmental analysis and target detection on a microscope image sequence, generating a mask for identifying the position of an interferent and an index for quantifying scene complexity, positioning sperms by the latter; the core is a self-adaptive trajectory tracking module which receives the two paths of information and performs tracking based on a dual feedback mechanism: on one hand, the correlation cost of a detection result in an interferent mask area is set as a maximum value, so that wrong matching is avoided; and on the other hand, the internal parameters of the tracking model are dynamically adjusted according to the scene complexity index. According to the method, dynamic parameters such as VCL, VSL, ALH and BCF are accurately calculated by generating the robust motion trail, a comprehensive report containing a quantitative result and analysis quality control evaluation is finally output, and the accuracy and credibility of an analysis result are remarkably improved.

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker synergistically related to growth and reproduction traits of chickens and application, and belongs to the technical field of molecular biology. The SNP molecular marker related to reproduction is located at the 28973618th nucleotide of chromosome 6 of a reference sequence of a GRCg6a version of a chicken genome, and has C / G polymorphism. The growth-related SNP molecular marker disclosed by the invention is positioned at the 2897383838th nucleotide of chromosome 6 of a reference sequence of a GRCg6a version of a chicken genome, and has G / A polymorphism. The sperm density and the keel length of a diplotype CCAA individual are the highest, and the growth traits and the reproduction traits are in a synergistic relationship. The SNP molecular marker disclosed by the invention can be used for carrying out marker-assisted selection on characters such as chicken sperm density and keel length, implementing early selection and improving the breeding efficiency. The invention provides a reliable molecular marker for the genetic improvement of chicken growth and reproduction traits, and has important significance for the genetic improvement of chicken.

Disclosed is a method for live detection of apoptotic sperms contained in a sperm sample under in vitro conditions.

The application discloses PRR30 gene SNP and a haplotype combination thereof in identifying bull semen quality, and belongs to the technical field of molecular genetics. Eight SNP sites are identified, wherein SNP7: g.794 C>A or SNP8: g.2247 G>T can be used for identifying the high and low of bull semen collection amount, SNP2: g.-2151 C>T can be used for identifying the high and low of bull sperm abnormality rate, and the haplotype combination of SNP1-SNP8 is that the bull with H1H11 has higher semen collection amount and lower sperm abnormality rate, and can be further used for identifying bull semen quality, thereby helping staff to evaluate bull breeding performance in an early stage, to carry out superior gene bull screening, to promote the cultivation of superior bull, to shorten the screening period and to improve the breeding efficiency.

The invention discloses an auxiliary reproductive semen treatment device matched with different insemination modes, which comprises a box body with an openable box door, a heating treatment table is arranged at the center of the bottom in the box body, and a treatment plate is placed on the heating treatment table; a control box is arranged at the top, a control circuit board is arranged in the control box, a heat dissipation plate and a display screen are arranged, and keys and knobs are arranged on the two sides; the back of the box is provided with a power interface. Through modular design, the device is compatible with semen treatment of various insemination modes such as IVF, ICSI and IUI; the first processing plate (transverse groove method) and the second processing plate (screen method) are used for screening sperms, through natural movement ability, form and thermotaxis guidance, mechanical damage is reduced, sperm motility is improved, sperm DNA fragment rate is reduced, and therefore ovum fertilization rate, embryo quality and pregnancy outcome are expected to be improved.

This invention relates to the field of beauty and skincare, and discloses an automated system and method for detecting semen quality, comprising: a morphological defect rate calculation module for calculating the morphological defect rate corresponding to a target semen sample; a dysfunctional sperm identification module for identifying dysfunctional sperm in the target semen sample; a reproductive function scoring module for evaluating the reproductive function score corresponding to the target semen sample; a dynamic motility potential scoring module for configuring a suitable culture medium for the target semen sample, culturing the target semen sample in the suitable culture medium, recording the sperm viability baseline parameters during the culture process, calculating the sperm survival rate of the target semen sample, and evaluating the dynamic motility potential score corresponding to the target semen sample; and a semen quality analysis module for generating a semen quality analysis report corresponding to the target semen sample. This invention can improve the accuracy of semen quality detection.

The present invention provides a carbomer copper gluconate complex hydrogel, a preparation method, and applications thereof. The carbomer copper gluconate complex hydrogel is prepared in 100 ml or 100 g portions. The raw materials, calculated by mass percentage, include: 0.01% to 0.1% carbomer copper gluconate complex (calculated as copper ion addition); 1% to 2% propylene glycol; 0.1% to 0.2% sodium hyaluronate; 2% to 6% dimethicone; and the balance is an acidity regulator and water. The carbomer copper gluconate complex hydrogel not only effectively kills sperm and common pathogens, but also protects the vaginal mucosa while providing contraception, avoiding the irritation and side effects of chemical spermicides.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The application belongs to the field of fish development, and particularly relates to a molecular marker for identifying Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring, a primer set thereof, a kit and application. The primer set for identifying the molecular marker of the Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring comprises primers F and R, the nucleotide sequence of the primer F is shown as SEQ ID NO. 1, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 2. Based on genomic data analysis, the application develops the molecular marker F / R which can effectively identify the Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring in the juvenile stage. The method comprises extracting genomic DNA of parents and offspring, PCR amplification and agarose gel electrophoresis detection, and finally distinguishing the gynogenesis individuals and hybrid individuals by whether the Nibea albiflora parent marker is contained in the electrophoresis band.

The invention discloses an application of a biomarker TDRD6 mutation in an auxiliary ovum activation technology aiming at non-disease diagnosis or treatment. The mRNA CDS sequence of the TDRD6 is as shown in SEQ ID NO. 1, and the mRNA CDS sequence of the TDRD6 is as shown in SEQ ID NO. Whether the TDRD6 is mutated or not is detected, the oocyte activation capacity of sperms in the fertilization process is judged, and whether the auxiliary ovum activation technology can be applied or not is guided. The invention also discloses a method for improving the normal fertility rate, the high-quality embryo rate and the living yield by using the biomarker TDRD6 mutation, and application of the biomarker TDRD6 mutation in preparation of medicines for improving the normal fertility rate, the high-quality embryo rate and the living yield. A drug / pharmaceutical composition for improving normal fertility rate, high quality embryo rate and live yield. The TDRD6 mutation and the application indication of the TDRD6 mutation as auxiliary ovum activation are beneficial to more accurate and efficient use of an auxiliary ovum activation technology, and the TDRD6 mutation is suitable for treatment application of target people conforming to the indication and has a wide application prospect.

The invention discloses a traditional Chinese medicine composition for preventing and treating light and moderate varicocele and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 12 to 30 parts of radix paeoniae rubra, 9 to 24 parts of peach kernel, 9 to 24 parts of spina gleditsiae, 9 to 24 parts of radix cyathulae, 6 to 18 parts of cassia twig, 9 to 24 parts of fructus forsythiae, 6 to 18 parts of thunberg fritillary bulb, 6 to 18 parts of rhodiola rosea, 6 to 18 parts of semen aesculi and 3 to 15 parts of raw liquorice. After the traditional Chinese medicine composition provided by the invention is adopted for treatment, the spermatovenous reflux time is remarkably shortened, the semen quality including semen volume, sperm density and sperm motility is remarkably improved, the total effective rate of treatment is higher than that of other prescriptions, the living quality of a patient is remarkably improved, the medicine safety is high, and the varicocele can be effectively treated.

An apparatus for diagnosing an embryo implantation potential using an artificial intelligence-based diagnostic model according to the present disclosure includes a memory storing a diagnostic model trained to determine an implantation potential index for an embryo based on an image generated by photographing one or more embryos formed by fertilization of a sperm and an oocyte and before implantation, and a processor for inputting a diagnostic image generated by photographing one or more first embryos, which are determination targets of the implantation potential index, into the diagnostic model as input data, and receiving implantation potential index data, which is a result of determining the implantation potential index for the first embryo, as output data from the diagnostic model.

The invention discloses an efficient extraction method of blue fox sperm DNA, and belongs to the technical field of molecular biology. Aiming at the structural characteristics that chromatin of the blue fox sperms is highly condensed, histone is replaced by protamine, and a stable nucleoprotein complex is formed through an intermolecular disulfide bond, the invention provides an efficient DNA extraction method suitable for the blue fox sperms. According to the method, based on the synergistic effect of three chemical reagents, namely SDS, PK and DTT, full lysis of the sperm cells of the blue foxes is achieved, and then efficient enrichment of sperm DNA of the blue foxes can be achieved by combining a conventional DNA extraction method. According to the efficient extraction method of the blue fox sperm DNA provided by the invention, the yield and integrity of the blue fox sperm DNA are remarkably improved, and the limitation that sperm chromatin is difficult to effectively lyse by a conventional method is overcome; the method provides reliable technical support for research on genetic diversity evaluation, population management, molecular breeding and the like of the blue foxes, and has the potential of popularization and application in other high-condensation sperm species.