60 results about "Spermatid" patented technology

An apparatus for diagnosing an embryo implantation potential using an artificial intelligence-based diagnostic model according to the present disclosure includes a memory storing a diagnostic model trained to determine an implantation potential index for an embryo based on an image generated by photographing one or more embryos formed by fertilization of a sperm and an oocyte and before implantation, and a processor for inputting a diagnostic image generated by photographing one or more first embryos, which are determination targets of the implantation potential index, into the diagnostic model as input data, and receiving implantation potential index data, which is a result of determining the implantation potential index for the first embryo, as output data from the diagnostic model.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

This invention discloses a pharmaceutical composition for treating asthenospermia by activating the Nrf2 / HO-1 / GPx4 pathway and its application. The pharmaceutical composition comprises taurine and geranium as active ingredients, wherein the mass ratio of taurine to geranium is 3-5:1. The pharmaceutical composition of this invention can synergistically activate the Nrf2 / HO-1 / GPx4 antioxidant signaling pathway, upregulate the expression of downstream antioxidant enzymes GPx4 and HO-1, inhibit lipid peroxidation, protect sperm mitochondrial function, and simultaneously improve the microenvironmental metabolic profile of the epididymis—the site of sperm maturation—thereby enhancing sperm motility. The pharmaceutical composition provided by this invention can be used to prepare a treatment for asthenospermia, particularly oxidative stress-induced asthenospermia.

The application discloses an information processing method and device for detecting sperm quality, equipment and medium, applied to a computer device, relates to the field of biomedical detection, and comprises the following steps: injecting an original semen sample into a preset microfluidic chip to dilute the original semen sample, and collecting multi-modal diluted sample data; performing parallel feature analysis on the multi-modal diluted sample data to determine corresponding multiple sperm characteristics; performing feature fusion on the multiple sperm characteristics based on a preset feature weight distribution network to obtain sperm fusion characteristics; determining corresponding several sperm quality parameters based on the sperm fusion characteristics, and determining the sperm quality of the original semen sample based on the several sperm quality parameters. Therefore, the detection efficiency and result reliability can be improved through highly automated synchronous multi-modal data acquisition and deep fusion analysis.

This utility model relates to the field of cryopreservation equipment technology and discloses a portable mouse sperm cryopreservation device, including a liquid nitrogen tank. A positioning component is fixedly connected to the inner wall of the liquid nitrogen tank, and a measuring rod is slidably connected inside the positioning component. A first foam float is fixedly connected to the bottom of the measuring rod. A hanging groove is provided on the top of the liquid nitrogen tank, and a hanging rod is hung inside the hanging groove. A telescopic rod is slidably connected inside the hanging rod, and a second foam float is fixedly connected to the bottom of the telescopic rod. A placement tray is fixedly connected to the outside of the second foam float, and a pressure cap is slidably connected to the outside of the telescopic rod. This portable mouse sperm cryopreservation device allows for a direct view of the liquid nitrogen level in the liquid nitrogen tank through the cooperation of the measuring rod and the first foam float. The placement tray is connected to the hanging rod via the telescopic rod and is equipped with a pressure cap and limiting components to securely fix the cryopreservation tube.

The application discloses a kit for determining anti-sperm antibody IgG and application thereof, and comprises sperm membrane antigen coated carboxyl magnetic beads, an analysis buffer and an enzyme working solution, wherein the sperm membrane antigen coated carboxyl magnetic beads are labeled with biotin, streptavidin and streptavidin antibody; in the application, the streptavidin antibody is added in the coating process of the carboxyl magnetic beads, a carboxyl magnetic bead-streptavidin antibody-streptavidin-biotinized antigen is formed, the connecting length between the antigen and the magnetic beads is increased, the steric hindrance is reduced, and the interference of some sample detection is avoided.

This invention discloses a microfluidic chip for three-stage non-destructive sperm screening and its usage method. The chip includes a substrate, a sample inlet channel layer, a sample chamber layer, a recovery chamber layer, and a screening filter membrane. The sample inlet channel layer has an inlet channel and an overflow channel. The sample chamber layer has an annular sample chamber, an inlet orifice, and an overflow orifice. The recovery chamber layer is divided into an outer recovery chamber and an inner recovery chamber by a dike. An inducing agent patch is located in the center of the inner recovery chamber. The screening filter membrane is sandwiched between the sample chamber layer and the recovery chamber layer. In use, after injecting a sperm sample, initial motility screening is achieved through the screening filter membrane. Secondary chemotactic screening is completed through an inducing agent concentration gradient. Tertiary adaptive screening for swimming direction is completed using the chemotactic channel. Finally, high-quality sperm are obtained from the inner recovery chamber. This invention achieves multi-parameter graded screening, effectively avoiding sperm damage, improving sperm recovery quality and screening efficiency, and is applicable to various assisted reproductive technologies, helping to improve conception success rates.

This invention discloses a Fam205a1 Methods for constructing and applying gene knockout mouse models. The construction method utilizes CRISPR / Cas9 gene editing technology, targeting mice. Fam205a1 Gene-designed specific sgRNAs for knockout Fam205a1 The regions of exons 1 through 4 of the gene are obtained. Fam205a1 Gene knockout mice. Male mice in this model exhibit infertility, specifically characterized by impaired spermatogenesis, reduced sperm motility, abnormal sperm morphology (particularly head and acrosome defects), and spermatogenic cell apoptosis. However, the weight and appearance of their testes and epididymis show no significant difference from the wild type. The mouse model constructed in this invention has stable genetic traits and a well-defined phenotype, making it an ideal animal model for studying the mechanisms of idiopathic male infertility, screening drugs for treating male infertility, or developing male contraceptives.

This invention provides a microfluidic organ-on-a-chip for sperm screening, comprising a chip body with a sample injection area, multiple detection channels, and multiple upstream recovery areas of chemotactic gradients. The number of detection channels and upstream recovery areas of chemotactic gradients are the same. The sample injection area and the multiple upstream recovery areas of chemotactic gradients are connected by multiple independent detection channels. Each detection channel includes at least a buffer zone, a microchamber, and a chemotactic gradient generation zone connected sequentially. The sample injection area and the buffer zone are connected by a distribution channel, and the buffer zone and the microchamber are connected by a narrowing channel. The chemotactic gradient generation zone includes a main chemotactic gradient generation channel and tributary channels. The main chemotactic gradient generation channel connects the microchamber and the upstream recovery areas of the chemotactic gradient. Multiple tributary channels are located on both sides of the main chemotactic gradient generation channel and connected to it. Sperm samples are added to the sample injection area, and a transparent glass slide is placed on the chip body to form a sealed structure.

This invention relates to the field of sperm detection technology, specifically disclosing an automated sperm density detection chamber integrating sample mixing and temperature control functions. The chamber comprises, from bottom to top, a light-transmitting bottom layer, a microchannel layer, and a light-transmitting cover layer. The light-transmitting bottom layer has, from bottom to top, a glass substrate layer, an ITO heating electrode layer, a first light-transmitting insulating layer, a first ITO mixing electrode layer, and a second light-transmitting insulating layer. The light-transmitting cover layer has, from top to bottom, an upper glass surface layer, a second ITO mixing electrode layer, and a third light-transmitting insulating layer. The microchannel layer forms microchannels, and the light-transmitting cover layer has an inlet port and an exhaust port communicating with the microchannels. This invention achieves stable constant temperature control of the semen sample within the microchannels, effectively avoiding the adverse effects of environmental temperature fluctuations on sperm characteristics and detection results. Furthermore, it enables in-situ automatic mixing of the semen sample, improving the accuracy and repeatability of sperm density detection results.

This invention discloses the application of Polygonatum oligosaccharides in the preparation of products that improve sperm motility. The molecular weight of the Polygonatum oligosaccharides is 1 × 10⁻⁶. 4 ~5×10 4 This oligosaccharide, Da, can be prepared through steps including ethanol defatting, water extraction, 30%-50% ethanol precipitation, and protein removal. Experiments show that this oligosaccharide can significantly improve sperm motility, particularly restoring the forward sperm motility rate in asthenospermia model mice to over 32%, reaching the clinical treatment threshold; it can also effectively alleviate cyclophosphamide-induced reproductive damage and improve testicular tissue pathological damage. Furthermore, this oligosaccharide can upregulate the expression of sperm motility-related genes such as Crisp2 and Catsper, downregulate miR-27 expression, and has multiple effects including reducing oxidative stress, inhibiting inflammation, and improving reproductive hormone levels. Therefore, this oligosaccharide can be used to prepare products that improve sperm motility, prevent and / or treat asthenospermia, and alleviate chemotherapy-related reproductive damage.

This invention relates to microfluidic devices, and more particularly to a sperm sorting device and a sperm guiding device. A sperm sorting device is provided, comprising: an inlet chamber, an outlet chamber located radially inside the inlet chamber, and a channel connecting the inlet and outlet chambers. The channel includes blind branches with a radius of curvature between 50 μm and 75 μm. A sperm guiding device is also provided, comprising an inlet opening and an outlet opening, and blind branches with a radius of curvature between 50 μm and 75 μm. The technical advantage of this invention is the reduction of sperm backflow.

The application discloses a pig IZUMO2 eukaryotic protein and a preparation method and application of a monoclonal antibody of the pig IZUMO2 eukaryotic protein. The full-length sequence of pig IZUMO2 nucleotide CDS is verified, a research method for removing the transmembrane structure of the pig IZUMO2 nucleotide CDS and only expressing the extramembrane sequence of the pig IZUMO2 nucleotide CDS in eukaryotes is designed, and the IZUMO2 protein is expressed and secreted by CHO-K1 cells. The protein expressed by the method is close to natural protein, has biological activity and is simple to operate. The qPCR and ELISA tests verify that the IZUMO2 protein can reduce the expression of inflammatory factors in RAW264.7 cells induced by LPS, and has a certain anti-inflammatory capacity. It is found through experiments that the IZUMO2 protein can promote the capacitation of boar sperm, and it is proved that the protein can be applied to the preparation of a reagent for promoting the capacitation of boar sperm.

This invention discloses a method for cryopreservation of donkey semen using antioxidants, belonging to the field of animal reproductive technology. The method is based on a specific basal diluent, with the addition of targeted mitochondrial antioxidants SKQ1 or SS-31. First, a basal diluent containing sugars, citrate, proline, skim milk powder, and antibiotics is prepared. Then, SKQ1 (dissolved in DMSO) or SS-31 (dissolved in physiological saline) is separately prepared into a stock solution, which is then mixed with the basal diluent to obtain a composite preservation solution. Finally, the donkey semen treated with this composite preservation solution is diluted and stored at 4°C. The method of this invention can significantly improve sperm motility and acrosome integrity rate of donkey semen stored at 4°C for 5 days, reduce ROS content and abnormality rate, and significantly increase the insemination and conception rate of the preserved semen to over 40%, effectively extending the practical preservation period of donkey semen.

The application belongs to the technical field of biology, and discloses a method for activating an ASXL2-PRC2 axis, wherein adenovirus is injected into the seminiferous tubule of an organism through an outgoing pipe to activate the expression of endogenous EZH2 and spermatocyte-specific ASXL2 in the organism. The method can be used for reducing impaired sperm motility, increasing sperm penetration and tubulin polymerization, inhibiting abnormal spermatogenesis, down-regulating the transcription level of a TZ marker, increasing the enrichment of SUZ12, EED and H3K27me3 in CEP162 and CEP290 promoters under hypoxic conditions, and slowing down hypoxia-induced abnormal spermatogenesis and spermatocyte activation of the ASXL2-PRC2 axis in the germ cell line of the organism.

The present application relates to the technical field of extracellular vesicle sorting, and particularly relates to a epididymal body specific sorting marker, and an epididymal body sorting kit and method. The present application finds that CD52 can be used as a specific marker for sorting epididymal bodies. The present application is the first time to directly separate epididymal bodies from human seminal plasma, the sample is easy to obtain, and the operation process is non-invasive, safe, and the separation path is simple and easy to operate. In addition, it provides a method for the separation of other tissue-specific EV subpopulations. The present application can contribute to further explore the effect of epididymal bodies on sperm function and improve sperm function, and epididymal bodies as a marker have great potential for clinical diagnosis and treatment.

This invention provides a fertilization ability information generation system, a fertilization ability information generation method, and a fertilization ability information generation computer program that can improve the accuracy of evaluating sperm fertilization ability. [Solution] The fertilization capacity information generation system 100 includes a short-axis image detection unit 124 that detects a short-axis image 210 consisting of two substantially parallel lines or one band-shaped line extending in the short-axis direction of the head in a sperm head image 200 obtained by capturing an image of at least the head of a sperm 90; a fertilization capacity information generation unit 125 that generates fertilization capacity information that can be used to evaluate fertilization capacity based on the detection result of the short-axis image 210 by the short-axis image detection unit 124; and a storage unit 123 and a display device 122 that store or output the fertilization capacity information generated by the fertilization capacity information generation unit 125.

This invention relates to the field of animal genetic engineering technology, providing a molecular marker for the ATP5PO gene associated with bovine sperm motility and its application. The ATP5PO gene molecular marker is located at position 203 of the bovine ATP5PO gene fragment, where an A-G base mutation occurs, resulting in a single nucleotide polymorphism (SNP). The nucleotide sequence of the bovine ATP5PO gene fragment is shown in SEQ ID NO:1. Based on this ATP5PO gene molecular marker, genotype can be determined through sequencing, and the genotype can be associated with normal and thawed bovine sperm motility. This invention utilizes a specific primer set to obtain the bovine ATP5PO gene fragment associated with normal and thawed bovine sperm motility, and uses specific SNP sites within this fragment as molecular markers, providing a theoretical basis and practical application for marker-assisted selection in cattle.

This invention belongs to the field of family planning, specifically relating to a sterile fertility-protecting condom suitable for pregnant women and its preparation method. The preparation method includes: medical-grade natural latex undergoing sequential deproteinization, purification, and defoaming treatments; then, it is molded and vulcanized to obtain a semi-finished condom; sodium hyaluronate is mixed with purified water to obtain an aqueous lubricant; the aqueous lubricant is coated onto the semi-finished condom, dried, and then sterilized by electron beam irradiation to obtain a sterilized semi-finished condom; the sterilized semi-finished condom is tested, and if qualified, packaged and stored to obtain the finished sterile fertility-protecting condom suitable for pregnant women. This invention solves the problems of traditional silicone oil condoms damaging sperm and sodium hyaluronate being intolerant to radiation, thus preventing unintended pregnancy while protecting fertility.

The present application is applicable to the field of in-vitro diagnostic devices. Disclosed is a sample analyzer. The sample analyzer comprises: a feeding device, a sample distribution device, a microscopic imaging device and a controller, wherein the controller is configured to: on the basis of an image obtained by means of the microscopic imaging device executing a first photographing action on a semen sample, output at least a motility analysis result of sperm in the semen sample; and on the basis of an image obtained by means of the microscopic imaging device executing a second photographing action on a first body fluid sample, output at least a morphological analysis result of at least some formed elements in the first body fluid sample. A first sample container and a second sample container are two mutually independent sample containers, and a first carrier and a second carrier are two mutually independent carriers or the same carrier. The sample analyzer of the present application can perform formed-element analysis on a semen sample and another body fluid sample, thereby facilitating a reduction in purchase, usage and maintenance costs of sample analyzers that can be used for semen sample analysis.

This utility model discloses a sperm cryopreservation tube for sealing, relating to the field of cryopreservation technology. The technical solution includes a tube body with symmetrically fixed blocks on its inner side. A cap is movably connected inside the tube body, and an inner sleeve is fixedly connected inside the cap. A pressing assembly is provided inside the inner sleeve, including a pressing pestle and a button. A sealing sheet is movably connected inside the cap, and a movable plate is fixedly connected to the outside of the sealing sheet. A second spring is fixedly connected to the outside of the movable plate, with one end of the second spring away from the movable plate fixedly connected to the inner wall of the cap. A groove is provided on the inner wall of the tube. By using the pressing assembly, the sealing sheet is pressed, causing it to slide on the inner sleeve and engage in the groove to seal the injection port of the tube body, preventing the cap from falling off and causing liquid nitrogen contamination. This simplifies the process of hand-held rotation when sealing the cap and tube body, quickly sealing the injection port of the tube body, preventing excessive air from entering the tube, and ensuring the sperm preservation effect.

The application discloses a kind of built-in anti-ejaculation devices of stallion, it is related to the technical field of animal husbandry, including placing hose, support tube is placed in the inside of placing hose;The outer side wall of the open end of support tube is installed with snap ring, and the opposite surface of snap ring is installed with fixed assembly;One of snap ring is in abutment with the inner side wall of placing hose, and the other snap ring is in abutment with the opening of placing hose.The application discloses a kind of built-in anti-ejaculation devices of stallion, not only can realize the filtration of sperm of stallion, but also can make that tissue fluid can normally flow and avoid vas deferens congestion, and will not affect normal mating behavior of stallion, further, anti-ejaculation behavior is reversible, does not affect stallion recovery reproductive capacity in later period, overall built-in structure design is compact, fixed assembly is assembled firmly, can be long-term retained and used, no exposed component, safe and non-irritating, adapt to stallion physiological structure, and practicality is strong.

1. The name of the design product: sperm quality analyzer (SQA100 portable). 2. The use of the design product: for sperm quality analyzer. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application discloses a preparation of a biological memristor and application of the biological memristor in sperm DNA breakage detection, and takes supernatant of a sample to be detected, and drops the supernatant on the surface of the memristor; after the memristor is connected with a detection platform, different voltage corresponding electrical signals are outputted, and the normal sperm and the sperm with DNA breakage are distinguished. The application has the characteristics of simple detection process, low cost, high sensitivity and fast response, and can quickly distinguish whether sperm DNA sequence breakage exists in sperm cells of a male patient with infertility.

Aspects of this invention relate to a novel monoclonal antibody specifically designed to exhibit selective binding affinity towards antigens present predominantly in X chromosome sperm. The antibody is engineered for applications to sex select sperm for cattle breeding purposes.

The application discloses a sperm protein marker LYPD4 closely related to human sperm hyperactivation movement and an application thereof, and belongs to the technical field of biology.The application first proves that the LYPD4 protein is closely related to human sperm hyperactivation movement, and the relative content of the LYPD4 protein is significantly positively correlated with the human sperm hyperactivation rate; the LYPD4 antibody can significantly inhibit the total activity, forward movement, hyperactivation movement, penetration mucus capacity and sperm capacitation level of human sperm.The application provides a new application of the LYPD4 as a sperm function evaluation biomarker, and can be used for preparing a human sperm hyperactivation capacity detection kit, screening high-quality sperm for assisted reproduction, male infertility diagnosis and therapeutic drugs.The application fills a research blank of specific molecular markers of sperm hyperactivation movement, provides a new target and a new method for male reproductive health diagnosis and treatment and assisted reproduction technology, and has important scientific research value and clinical application prospect.

The utility model relates to a porous plate sperm screening device based on upstream method, including porous plate base and sperm screening spare, the body middle part recessed and recessed bottom interval of porous plate base is provided with two or more than two culture holes, and each culture hole is provided with a sperm screening spare in a matched mode, the main part of sperm screening spare is embedded in the culture hole, and the culture hole wall top is sleeved in through the sleeve setting of sperm screening spare top, the main part bottom of sperm screening spare sets up the annular buffer ring groove that forms relative to the main part of sperm screening spare and extends inwards, and multiple channels are arranged on the inner side groove wall of buffer ring groove, and the main part bottom of sperm screening spare forms the communicating port in the inner side of buffer ring groove, and the filter membrane that seals the communicating port and is used for sperm upstream is arranged at the communicating port, at least one concave sampling groove is arranged at the bottom of buffer ring groove.

This invention discloses an in vivo sperm processing device, control method, and application based on surface acoustic wave (SAW) devices. The non-invasive in vivo sperm processing method based on SAW devices offers significant advantages: it avoids the trauma risks of surgical and acupuncture treatments and solves the problem of unstable effects from hormone therapy and antioxidant therapy. By precisely controlling SAW parameters, this invention significantly improves sperm motility. It is simple to operate, low in cost, and highly safe, and applicable to various types of oligospermia and asthenospermia, providing a highly efficient, non-invasive, and widely applicable new solution for the research and treatment of oligospermia and asthenospermia.

This disclosure describes proteins that specifically bind human sperm protein 17 (Sp17) with nanomolar affinity and high specificity. These proteins include a recombinant human anti-Sp17 IgG that is suitable for use as a therapeutic antibody to treat cancers that ectopically express Sp17. Other Sp17-binding proteins are described including antibody fragments, antibody conjugates, and fusion proteins.

The application belongs to the technical field of biological medicine, and particularly relates to application of a reagent for inhibiting PPAR alpha expression in preparation of a medicine for treating male infertility diseases, wherein a PPAR alpha inhibitor (GW6471) or an active oxygen scavenger (NAC) is given to male mice exposed to cadmium water, it is found that the number of sperm in a Cd+GW6471 group is higher than that in a Cd+NAC group, the serum testosterone level in the Cd+GW6471 group is also higher than that in the Cd+NAC group, and the results of testicular tissue pathological staining also show that GW6471 can more significantly restore the decrease in the number of mature spermatogenic tubules caused by Cd, GW6471 restores the decrease in the number of SYCP3 positive spermatocytes caused by Cd, so that the sperm production efficiency is restored, and therefore the application of the reagent for inhibiting PPAR alpha expression in preparation of the medicine for treating male infertility diseases is proposed.