972 results about "Spermatid" patented technology

The present invention relates to a method and device for male contraception. In one aspect, the method and device provide temporal and reversible infertility of mammal males without suppressing spermatogenesis. In an aspect, a method of contraception for use with reproductive cells that pass through a tube in a body is described in which energy is applied through the tube wall to some of the reproductive cells that pass through the tube to reduce fertilization ability of the some of the reproductive cells. In another aspect, the device is adapted for use with reproductive cells that pass through a tube in a body, and includes an energy transmitter that delivers energy to the reproductive cells that pass through the tube in the body.

The present invention provides methods for preparing cells with highly condensed chromosomes, such as sperm, and methods for detecting and quantifying specific cellular target molecules in intact cells. Specifically, methods are provided for detecting chromosomes and chromosomal abnormalities, including aneuploidy, in intact cells using fluorescence in situ hybridization of cells in suspension, such as sperm cells.

This invention describes unique patterns of distribution of ganglioside G_M1 in non-capacitated sperm and demonstrates that the pattern of distribution of G_M1 undergoes changes that can be correlated with the process of capacitation and/or with acrosomal exocytosis. Accordingly, the present invention discloses a method for determining the ability of sperm to respond to capacitation and/or acrosomal exocytosis stimuli. The method comprises determination of distribution pattern for G_M1. The method can be used for both diagnostic and predictive purposes when assessing male reproductive fitness, and can also be used to assess the effects of any agent or environment on sperm including cryoprotective agents and protocols, and contraceptive agents.

The invention discloses a special feed additive for conditioning the breeding performance of artificially inseminated breeder cocks and application thereof. The special feed additive for conditioning the breeding performance of artificially inseminated breeder cocks is prepared from a composite traditional Chinese herbal additive and a nutritional feed additive according to a weight ratio of 1:1, wherein the composite traditional Chinese herbal additive comprises 7-10 percent of epimedium, 7-10 percent of actinolite, 7-10 percent of yam, 7-10 percent of prepared rhizome of rehmanniat, 5-8 percent of medlar, 5-8 percent of cistanche salsa, 5-8 percent of dodder, 5-8 percent of raspberry, 5-8 percent of plantain seeds, 5-8 percent of japanese teasel, 5-8 percent of liriope, 3-4 percent of Dangshen, 3-4 percent of morinda officinalis, 3-4 percent of astragalus, 3-4 percent of angelica sinensis, 3-4 percent of atractylodes and 3-4 percent of glycyrrhiza, and the percent sum of the raw materials is 100 percent. The nutritional feed additive comprises 35-45 percent of alfalfa meal, 20-25 percent of folium cortex eucommiae powder, 20-25 percent of mulberry leaves powder, 4-9 percent of bone meal, 5-10 percent of saleratus and 0.5-1 percent of trace elements and composite vitamin additive, and the percent sum of raw materials is 100 percent. After the special feed additive is applied, the average ejaculate volume of breeder cocks is improved by 17.6 percent, the sperm activity is improved by 16.1 percent, and the sperm density is improved by 14.2 percent.

Described herein is a composition that comprises a cryoprotectant; a membrane protectant that stabilizes or assists in stabilization of membranes of sperm; and a free radical scavenger (e.g., a reducing agent, an antioxidant).

The invention belongs to the technical field of livestock molecular marker preparation, and concretely relates to a molecular marker related to a sperm activity character of a boar and application. The molecular marker is screened from a PRM2 gene; a nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. An allelic mutation R exists in nucleotide in position 169 of a sequence table, R is G or A, and the mutation causes the polymorphism of NaeI-RFLP. The invention further discloses a primer pair for amplifying PRM2 gene SNP (Single Nucleotide Polymorphism), and the primer pair is also the primer pair for detecting the screened molecular marker provided by the invention. The invention builds a method for detecting the polymorphisms of the sperm activity character of the boar. Association analytic application shows that the molecular marker provided by the invention can be used as the marker for assistant selection of the sperm activity character of the boar.

The invention relates to Chinese herbal medicine feed additive for stud rams, which mainly solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art. The Chinese herbal medicine feed additive is prepared by crashing and mixing epimedium, actinolite, Chinese dodder seed, raspberry, Chinese magnoliavine fruit, astragalus root and dangshen and can be added into feed for usage. The invention improves ram sexual appetite, prompts spermatogenesis, increases sperm motility, effectively improves reproductive performance, solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art according to spermatogenesis principle.

The invention discloses a health-care food for males and a preparation method thereof. The health-care food is prepared according to a traditional Chinese medicine theory and the respective health-care efficacies and characteristics of maca, cistanche and liquorice; maca has the efficacies of resisting fatigues, improving sexual functions, increasing the number of sperms, improving the quality of the sperms, reducing benign prostatic hyperplasia, enhancing fertility and the like; traditional Chinese medicine cistanche has the efficacies of benefiting essence and blood, tonifying the kidney and reinforcing yang as well as lubricating intestines and smoothing bowels and can have health-care effects on kidney deficiency and impotence, spermatorrhea and premature ejaculation, coldness and pain of a waist and knees and the like; liquorice is a tonifying Chinese herbal medicine and can coordinate drug properties as well as tonify spleen and benefit qi. By virtue of a nanotechnology, the bioavailability of bioactive molecules in maca, cistanche and liquorice is improved; by virtue of fine processing, maca, cistanche and liquorice are prepared into the health-care food for males; the health-care food is safe and effective, nontoxic and harmless, and has synergistic effects on improvement of sexual function, enhancement of fertility, regulation of internal secretion, reduction of prostate diseases and improvement of the immunologic function of an organism.

The invention discloses a cryopreservation method for sperms of ablen, which comprises the following steps of: sampling the sperms, performing microscopic examination, mixing the sperms and an antifreeze agent, and freezing. The cryopreservation method is simple and feasible, has obvious effect and good cryopreservation effect. If the sperms of the ablen most important in Chinese aquiculture are preserved by the method, the activity of the unfrozen sperms is more than 90 percent, and the bottleneck problems of insufficient male fish and asynchronous sexual maturity of female and male parent fish in the ablen culture are effectively solved.

The invention which belongs to the medical field of genetic engineering and reproduction discloses a seminal plasma miRNA marker associated with human non-obstructive azoospermia and an application thereof. The maker is selected from several of hsa-miR-141, hsa-miR-193a-5p, hsa-miR-590-5p and hsa-miR-7-1*. The maker which has specificities and sensitivities to the non-obstructive azoospermia can be used for the preparation of a reagent for non-obstructive azoospermia diagnosis or monitoring, so invasive diagnosis is avoided, repeated detection is realized, and the dynamic monitoring of the obstacle degree of sperm generation can be easily achieved.

The invention discloses a boar semen refrigerating method and dedicated refrigerating fluid thereof. The method comprises the following steps: (1) diluting dense boar semen according to a volume ratio of 1:(0.5-3), stewing at 26-30 DEG C for 25-40 minutes, and centrifuging 800g of boar semen for 10 minutes to obtain sperm precipitation; (2) adding refrigerating fluid I into the sperm precipitation according to a volume ratio of 1: (1-1.5), stewing at 17-20 DEG C for 1-2 hours, and stewing at 4-7 DEG C for 2 hours to obtain a system I; (3) adding refrigerating fluid II containing glycerin into the system I to obtain a system II with final glycerin concentration of 3%; (4) stewing the system II at 4 DEG C for 1-2 hours, and adding caffeine to obtain a system III with final caffeine concentration of 0.2g/L; and (5) stewing the system III at 4 DEG C for 0.5-2 hours, dropwise refrigerating at -120 DEG C, stewing and balancing for 5-10 minutes, and submerging the system III in liquid nitrogen for cryopreservation. After the boar semen refrigerated by the method is thawed, the sperm survival rate of the boar semen is higher than 0.45, and the in vitro survival time of the boar semen is longer than 6 hours, thereby reaching the production application level. The boar semen refrigerating method disclosed by the invention is used for providing scientific basis for the application and popularization of the refrigerated boar semen in production, and providing a technical method and standard for boar semen refrigerating.

The invention relates to a sperm and ovum health beverage which comprises fulvic acid, gene vitality element, plant extract, fungus polysaccharide, vitamin, hydroxycitric acid (HCA), soluble dietary fiber, L-arginine, L-carnitine, zinc gluconate, calcium lactate, sweetening agent, milk, coffee and purified water. The invention has simple technology, low cost and complete product nutrition and contains rich natural polypeptide, polysaccharide, amino acid and vitamin and various trace elements. All components interact to promote blood circulation and sex hormone secretion so as to achieve the functions of promoting the growth of sperms and ovums, improving the quality and the vitality of the sperms and the ovums and improving the sexual function. The sperm and ovum health beverage has the good improving effect on ED symptoms including renal dysfunction, working strain, heavy spirit pressure, bradypsychia, palpitation, stomachache, weak waist and knee, sexual dysfunction, and the like. As a pure natural preparation, the sperm and ovum health beverage has no poison and side effect and adverse reaction.

The present invention is a sperm collector with squeezing function. It includes a soft rubber body provided with a penis insertion passage and a container body provided with at least a top end cover and receives the soft rubber body. When the top end cover and the container body are engaged, the soft rubber body is confined within a cavity formed by the top end cover and the container body. When the top end cover is disengaged, a front end portion of the soft rubber body protrudes out of an opening of the container body. A pressing means is provided at a middle lower portion of the container body. When using the present invention, the user could press the pressing means to squeeze the penis insertion passage of the sperm collector. Accordingly, stimulation to penis is increased and better effect of sperm collection is achieved. In particular, since the present invention is also provided with a heating rod, lubricant pockets and electrical vibrators, which enable the user to reach a climax as quick as possible, thereby further enhancing the effect of sperm collection.

The invention relates to a medicine for treating male oligozoospermia and improving semen quality. Various natural edible plant medicines such as Rhizoma Polygonati, Fructus Lycii, Cordyceps militaris (L.) Link., Fructus Rubi, Fructus Mori, Fructus Hippophae, Semen Coicis, Radix Ginseng, and Concha Ostreae (calcined) serve as raw materials, are combined suitably, and jointly have effects of invigorating the spleen and replenishing Qi, and nourishing the kidney and replenishing essence, Lepidium Meyenii contains macaenes and macamides which are used as active substances has effects of promoting sexual function, increasing the number of sperms and improving activity of the sperms, selenium yeast, aminoacid, L-carnitine tartrate, calcium carbonate and fructose can strengthen and protect energy metabolism of the sperms, taurine and vitamin effectively remove reactive oxide species and protect activity of the sperms, and folacin can promote maturity of the sperms and improve functions of the sperms. The medicine is reasonable in composition, and the synergistic effect of various components is utilized, so that the medicine is remarkable and lasting in treatment effect, safe and reliable and convenient to take orally, and can effectively promote spermatogenesis, improve survival rate of the sperms and improve activity of the sperms.

After sampling semen from healthy males and from patients whose chief complaint was infertility, the semen volume, sperm concentration, sperm motility rate, and sperm survival rate in each of the specimens were measured, sperm-bound SPMI was detected using an anti-SPMI antibody, and the amount and rate of SPMI binding were calculated. As a result, only patient specimens were found in the specimen group with amount and rate of SPMI binding above a certain level. Diagnosis of asthenozoospermia can be made by determining the amount and rate of SPMI binding.

The invention provides a sperm screening and in vitro fertilization bionic device based on micro fluidic technology. The sperm screening and in vitro fertilization bionic device based on micro fluidic technology comprises a bionic device main body; the bionic device main body is composed of a glass sheet layer, a silica gel pipeline layer, and a polydimethylsiloxane layer; a center cell is arranged at the middle part of the silica gel pipeline layer; an inlet cell, a first outlet cell, and a second outlet cell are arranged outside the center cell; the center cell is communicated with the inlet cell, the first outlet cell, and the second outlet cell via a first pipeline, a second pipeline, and a third pipeline respectively; the first pipeline is provided with a first sample adding port; the second pipeline is provided with a second sample adding port; and the third pipeline is provided with a third sample adding port. The sperm screening and in vitro fertilization bionic device is used for stimulating complete natural impregnation process, unfavorable influences of operation on gametes and embryos are avoided, and polyspermy is avoided; high quality embryos at stimulated natural impregnation states can be obtained, in vitro fertilization success rate is increased greatly, the potential causes of sterility can be identified, and a direction is provided for clinical treatment.

The invention discloses a preparation method of a frozen diluent of sheep semen and an application of the preparation method. The preparation method of the frozen diluent of the sheep semen comprisesthe following steps: firstly, accurately weighing tris(hydroxymethyl) aminoethane, dihydrate trisodium citrate, fructose, an inositol compound, ethanediol or glycerinum, and oxytocin, adding the materials into ultrapure water, dissolving, adding a fresh yolk centrifuged liquid supernatant, penicillin and streptomycin, mixing evenly, fixing the volume after adjusting the pH value to 6.8-7.2, thus obtaining the frozen diluent of the sheep semen. The inositol compound is used to replace an antifreeze protein, the defect of the application of the antifreeze protein is avoided, meanwhile, the oxytocin is added when the semen is conveyed, and the sperm vitality and the acrosomal integrity are improved, so that the fertility rate is increased.

The invention discloses a mixed feed for improving antioxidant activity of the sperm of a boar. The mixed feed is characterized by comprising the following raw materials by weight: 40 to 60% of corn, 5 to 20% of fish meal, 5 to 20% of wheat, 10 to 25% of wheat bran, 0.2 to 5% of a soybean small peptide additive, 0.05 to 2% of taurine, 2 to 10% of soybean meal, 2 to 8% of plant oil, 1 to 2% of a calcium element additive, 0.1 to 0.8% of salt, 0.05 to 0.5% of N-carbamylglutamic acid, 0.1 to 0.3% of a composite trace element additive, 0.03 to 0.08% of a composite vitamin additive, 0.005 to 1% of anthocyanidin, 0.05 to 2% of tea polyphenol, 0.5 to 1.5% of amino acid and 0 to 5% of bentonite. The mixed feed is applicable to the boar, can effectively increase the amount of boar seminal fluid, improve sperm vitality and sperm density, reduce the rate of sperm deformation and enhance antioxidant activity of the sperm of the boar and is of great significance to improvement of the quality of boar seminal fluid.

A sperm collection device includes an open/close unit including two open/close members openably and closably supported about a hinge and core pieces supported by the open/close members. The core pieces are configured to open and close with the open/close members and to form an insertion space when the open/close members are closed.

The invention discloses a coated carrier and the application thereof in the method for detecting maturity of sperms and separating mature sperms noninvasively; the coated carrier is a carrier coated by hyaluronic acid, or hyalurate or hyaluronic acid derivatives; the detecting method is as follows: preparing specimens; loading the specimens to the coated carrier and covering with microscopic glass; reacting for 5-60 minutes at room temperature; observing by a microscope and counting; and calculating the combination ratio of hyaluronic acid of active sperms. The separating method is as follows: preparing specimens; loading the specimens to the coated carrier, reacting for 5-120 minutes at the temperature of 18-37 DEG C; and collecting the combined sperms. The coated carrier has the advantages of precise and reliable results, simple and fast operation, low cost and good safety performance, and can be used for estimating sperms quality, sperms maturity and analysis of the causes of sterility disease of mails, and is applicable to assisting reproduction or reproduction research center to safely collect mature and active sperms, so that the goal of improving embryo quality greatly is achieved.