1013 results about "Spermatid" patented technology

The sperm collection device includes a core member having an insertion hole for a penis and an insertion space, and a container having an internal space in which the core member is accommodated. The container includes a cylindrical base unit having a base end open in an axial direction of the base unit and attached to an insertion-hole-side end of the core member, and a cylindrical movable unit having a base end open in an axial direction of the movable unit and a front end being closed, and attached rotatably around an axis of the movable unit with respect to the other end of the base unit. In an overlapping part in which the base end of the movable unit overlaps the other end of the base unit, an airflow path and an adjustment mechanism are provided.

This disclosure describes proteins that specifically bind human sperm protein 17 (Sp17) with nanomolar affinity and high specificity. These proteins include a recombinant human anti-Sp17 IgG that is suitable for use as a therapeutic antibody to treat cancers that ectopically express Sp17. Other Sp17-binding proteins are described including antibody fragments, antibody conjugates, and fusion proteins.

This disclosure describes proteins that specifically bind human sperm protein 17 (Sp17) with nanomolar affinity and high specificity. These proteins include a recombinant human anti-Sp17 IgG that is suitable for use as a therapeutic antibody to treat cancers that ectopically express Sp17. Other Sp17-binding proteins are described including antibody fragments, antibody conjugates, and fusion proteins.

This disclosure relates to homodimeric antibodies for use in treating cancer generally and to both homodimeric antibodies that bind human sperm protein 17 (Sp17) and homodimeric antibody immunoconjugates specifically. Such homodimeric antibodies display improved properties relative to monomeric antibodies, for example, because they can crosslink cells, improve cellular uptake, and / or carry greater payloads.

This disclosure relates to dimeric immunoconjugates for use in treating cancer generally and to dimeric immunoconjugates that bind human sperm protein 17 (Sp17) specifically. A dimeric immunoconjugate of this disclosure generally comprises a first monomeric antibody that carries a first payload and a second monomeric antibody that carries a second payload, wherein the first payload and the second payload are different. Such dimeric immunoconjugates advantageously allow the simultaneous delivery of two chemotherapeutics to a cancer cell, which allows for synergistic antineoplastic activity.

This disclosure describes proteins that specifically bind human sperm protein 17 (Sp17) with nanomolar affinity and high specificity. These proteins include a recombinant human anti-Sp17 IgG that is suitable for use as a therapeutic antibody to treat cancers that ectopically express Sp17. Other Sp17-binding proteins are described including antibody fragments, antibody conjugates, and fusion proteins.

The invention belongs to the technical field of molecular biology and genetics, and particularly relates to a method for editing cattle genes based on Pro-iCHI. The invention provides a method for editing cattle genes based on Pro-iCHI, which comprises the following steps: transiently expressing protamine in cattle AG-haESCs after gene editing, and injecting into mature oocytes to obtain reconstructed embryos. The protamine can remove abnormal DNA methylation generated by an oocyte cytoplasm injection haploid stem cell technology, cell nucleuses are compressed into a sperm-like structure, the obtained bovine Pro-iCHI embryo can smoothly develop into a blastocyst, and the ratio of the blastocyst to the blastocyst obtained by in-vitro fertilization is equivalent. Moreover, the protamine coding gene is inserted into the saccharomyces cerevisiae protein expression vector and then is subjected to transient expression, so that the protamine coding gene cannot be integrated into a genome to cause insertion of an exogenous gene while abnormal DNA methylation is erased.

The invention provides application of a protein deacetylase inhibitor NAM in ultralow-temperature cryopreservation of poultry semen, and belongs to the technical field of poultry breed conservation. The protein deacetylase inhibitor NAM is used as a cryoprotectant or is combined with other cryoprotectants for use, and the protein deacetylase inhibitor NAM is used as an effective component, so that the ultralow-temperature cryopreservation efficiency of poultry can be improved. Wherein the protein deacetylase inhibitor NAM can inhibit sperm cell deacetylation modification so as to maintain a high acetylation modification level, improve the ability of sperms to respond to ultralow temperature freezing stimulation and improve the fertilization ability of frozen-remelted sperms.

The invention provides an ecology-imitating artificial propagation method for Dongquasichthys nobilis. The ecology-imitating artificial propagation method comprises the following steps: S1, selecting male and female parent fishes and putting the male and female parent fishes into an artificial ecology-imitating breeding pond for breeding; s2, in a non-breeding season, feeding female parent fish feed A for 2-3 times every day, and feeding male parent fish feed B for 2-3 times every day; s3, 2-3 months before breeding, female parent fish fortified feed I is fed 2-3 times every day, and male parent fish fortified feed II is fed 2-3 times every day; s4, after the gonad of the parent fish is mature, artificial spawning induction is conducted, and fertilized eggs are collected and hatched. According to the method, the ecology-imitating breeding environment is manufactured, so that the east astrostroma parent fish adapts to the artificial environment, the irritability is reduced, the east astrostroma parent fish is fed with different feeds in the non-breeding season and the breeding season, the ecological factor and nutritional requirements for gonad development of the east astrostroma can be met, the immunity of the east astrostroma parent fish is improved, gonad maturation is promoted, and the survival rate of the east astrostroma parent fish is increased. The quality of sperms and ova is improved, the fertilization probability is improved, the malformation probability of hatched larvae is reduced, and the breeding efficiency is improved.

The invention discloses an HNRNPA2B1 gene-based bovine sperm malformation rate related SNP molecular marker and application thereof, and belongs to the technical field of genetic molecular biology, the number of the SNP molecular markers is two, SNP1 is at the position of a bovine genome chr4: 69733358, the polymorphism is C or A, SNP2 is at the position of a bovine genome chr4: 69733387, and the polymorphism is T or G. According to the two SNP loci provided by the invention, the dominant genotypes of g.-1713A > C and g.-1684G > T are AA and GG respectively, and the sperm collection quantity and the sperm malformation rate of the two SNP loci have better performance. By detecting the polymorphism of the two SNP loci, the motility evaluation of the bull sperms can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.

The invention relates to the technical field of multi-modal fusion, in particular to a sperm quality assessment method and system based on multi-modal fusion, and the method comprises the following steps: obtaining image and parameter data to generate a space-time alignment set, extracting trajectory calculation linearity, analyzing parameter fluctuation to mark an abnormal time window, extracting an image assessment deformity rate to generate a feature matrix, and grading to generate a multi-modal sperm quality evaluation result. According to the method, linear calculation is introduced in the trajectory path extraction process, spatial distribution thermodynamic mapping is combined, the dynamic characteristics of local sperm populations are dominated, an abnormal time window is constructed based on the parameter value change rate and a set threshold value, and motion consistency indexes are combined, so that the dynamic characteristics of the local sperm populations are optimized. The explanation depth of sperm head malformation data in quality judgment is enhanced, the dimension space of sperm quality description is effectively expanded, the consistency ratio between vitality and morphology is introduced, track continuous characteristics are combined, a hierarchical grading system is established, and the accuracy of sperm quality evaluation results is ensured.

The invention discloses a method for efficiently and artificially inducing triploid grass carps, which comprises the following steps: (1) collecting sperms and ova of grass carps, and carrying out artificial insemination; (2) carrying out cold shock treatment on the fertilized eggs of the grass carps, wherein the cold shock treatment is that the fertilized eggs are placed in water at the temperature of 4-6 DEG C for 10-12 minutes; and incubating the fertilized eggs to obtain the triploid grass carp.

The invention belongs to the technical field of biological medicines, and particularly relates to a sperm injury repair model and an establishment method thereof. Comprising the following steps: step 1, establishing a sperm lead poisoning model; and 2, establishing a lead poisoning sperm repair model. The method for establishing the lead poisoning sperm repair model in the step 2 comprises the following steps: (1) preparing a preparation for optimizing sperm functions in an extracellular vesicle way; (2) repairing the damaged sperms by using the preparation for optimizing the sperm function through the extracellular vesicles; and (3) detecting the activity of the repaired sperms. In the invention, by establishing a sperm injury repair model and adopting an extracellular vesicle modification technology, the key functional protein is introduced into the extracellular vesicle, and the method has important significance on auxiliary researches such as development and research of related drugs for improving sperm injury and improving sperm motility of asthenospermia patients in vitro.

A method for automated, artificial-intelligence-based IVF microtool control includes receiving a command at a controller associated with an artificial intelligence / machine learning system (AI / ML system) and an imaging system used at least in part to operate robotic components of in vitro fertilization (IVF) module. The method includes retrieving at least one IVF microtool assembly (MA) from a tool inventory location using a first robotic mechanism of the robotics system, based at least in part on the command. The method includes retrieving at least one IVF receptacle using a second robotic mechanism of the robotics system based at least in part on the command. The method includes placing the IVF receptacle on a stage within the IVF module based on the command. The method includes positioning the at least one MA in proximity to the IVF receptacle, using the first robotic mechanism, based at least in part on the command.

The invention relates to a sperm cell analysis and diagnosis system based on a multi-modal large language model, which comprises a multi-modal data co-processing unit, a cross-modal semantic alignment module, a dynamic diagnosis decision engine and a self-adaptive evolution system, the four-dimensional data processing module is used for synchronously processing microscopic images, motion trail videos, biochemical detection data and four-dimensional input data of medical record texts and comprises a feature selector based on a gating attention mechanism. According to the sperm cell analysis and diagnosis system based on the multi-modal large language model, quantitative analysis of sperm movement chaos features is realized for the first time, a nonlinear dynamic evaluation standard is established, a cross-modal knowledge distillation and meta-learning migration framework is developed, a data annotation dependence bottleneck is broken through, and an interpretable clinical decision support system is constructed; dynamic updating and probabilistic suggestion of diagnosis rules are achieved, semantic analysis of single-cell multi-omics data is achieved, and molecular mechanism research results are converted into clinically available knowledge.

The invention provides a Wujin pig seminal plasma protein crude extract and application of the Wujin pig seminal plasma protein crude extract in an anti-freezing agent and an anti-freezing diluent for freeze preservation of Wujin pig seminal fluid. The Wujin pig seminal plasma protein crude extract is derived from Wujin pig seminal plasma, does not contain other exogenous bioactive substances, has a better freezing protection effect on sperms of Wujin pigs and is safer, and the acrosome integrity rate of frozen sperms of the Wujin pigs can be remarkably increased. According to the present invention, with the anti-freezing diluent, the vitality and the movement performance of the cryopreserved Wujin pig sperm can be significantly improved, the active oxygen level is reduced, the mitochondrial membrane potential and the energy metabolism are improved, and the oxidation resistance is enhanced, such that the vitality of the Wujin pig sperm can be easily improved, and the mitochondrial function can be easily maintained; the research provides key technical support for establishment of a Wujin pig semen cryopreservation technology, and has great practical significance for Wujin pig breeding and population propagation.

The invention discloses a pig sperm specific antibody. The coding sequence of the antibody is shown as any one of SEQ ID No: 1-3. According to the method, the nano antibody library is prepared by immunizing alpaca with whole pig sperms, and then the antibody capable of being specifically combined with the X or Y sperms can be screened out through the library, so that sorting of the X and Y sperms is realized. The screened antibody is a nano antibody, the size of the nano antibody is only 10% of that of a traditional antibody, the structure is simpler, the advantages of high stability, strong specificity and low immunogenicity are achieved, large-scale expression of the antibody can be achieved, the production cost of the antibody is greatly reduced, and industrial application is facilitated.

The invention provides a feed domesticated siniperca chuatsi sperm low-temperature preservation and ultralow-temperature preservation method, which comprises the following steps: firstly, providing a siniperca chuatsi sperm low-temperature preservation diluent which comprises Hanks liquid, sodium chloride, soya bean lecithin, vitamin E, curcumin, melatonin and SOD (superoxide dismutase); and adding an anti-freezing protective agent into the low-temperature preservation diluent to obtain the ultralow-temperature preservation anti-freezing liquid for the siniperca chuatsi sperms. The siniperca chuatsi semen and the low-temperature preservation diluent are uniformly mixed, so that low-temperature preservation can be realized; the siniperca chuatsi semen is uniformly mixed with the cryopreservation anti-freezing liquid, so that cryopreservation can be realized. The siniperca chuatsi sperm low-temperature preservation diluent is preferably selected, low-temperature preservation of the siniperca chuatsi sperms can be achieved, ultralow-temperature preservation can be conducted after an anti-freezing protective agent is added, the persistence of the preservation time of the siniperca chuatsi sperms can be guaranteed, the protection effect on the sperms is good, and the activation rate and activity of the unfrozen sperms are high.

The invention discloses a sperm real-time tracking and segmentation method based on a multi-task cascade analysis network, and the method comprises the following steps: S1, obtaining a sperm bright field image under a microscope, and carrying out the preprocessing of the sperm bright field image; s2, a detection module is adopted to carry out sperm head positioning detection on the preprocessed image, and rapid detection of a high-resolution sperm image is realized through combination of the improved detection module and a self-developed attention mixed feature down-sampler; s3, a sperm head detection result is cut and input to the lightweight real-time segmentation network for real-time segmentation, the model parameter quantity is significantly reduced, and the segmentation precision is improved; and S4, outputting a real-time tracking result to the sperm target in the continuous frames by using a tracking algorithm, and measuring and calculating the activity parameter of the target sperm. Through joint optimization of detection, segmentation and data enhancement modules, an efficient and robust automatic solution is provided for the assisted reproduction technology, and the method can be widely applied to sperm real-time analysis and screening in the assisted reproduction technology.

The invention relates to the technical field of microorganisms, and provides Witzia ciliaris KY009 (preservation number is CGMCC (China General Microbiological Culture Collection Center) NO. 33480) and Clostridium butyricum KY002 (preservation number is CGMCC NO. 33478), and the two strains are separated from an excrement sample. Experiments prove that the two strains can obviously improve the sperm quality and quantity, regulate the sex hormone level, improve the intestinal inflammation symptom and regulate the expression of related inflammatory factors. The invention also provides a composition containing the two strains, wherein the composition comprises a food composition, a pharmaceutical composition, a health-care product composition, a functional microbial agent and the like. The compositions are useful for improving reproductive health, modulating sex hormone levels, treating intestinal inflammation and promoting mucosal repair, and alleviating metabolic and immune related diseases. The strain and the composition provided by the invention have wide application prospects in the fields of medicines, foods and health-care products.

The invention provides application of a sperm tRNA (transfer ribonucleic acid) derived fragment 5 'tRF-Glu-CTC in prediction of development potential of assisted reproductive embryos. The expression of a tRNA derived fragment 5 'tRF-Glu-CTC in a sperm sample is detected, the abnormal expression of the sperm specific tRNA derived fragment 5' tRF-Glu-CTC in a DFI increase patient is verified, and the analysis on the correlation with clinical data and reproductive outcome of the patient prompts that the 5 'tRF-Glu-CTC molecular marker has significant correlation with the reproductive outcome, so that the 5' tRF-Glu-CTC molecular marker can be used for detecting the expression of the sperm specific tRNA derived fragment 5 'tRF-Glu-CTC in the DFI increase patient. The embryonic development potential can be effectively predicted, and a biomarker is provided for assisted reproduction treatment. In different experimental groups, the 5 'tRF-Glu-CTC can significantly improve the prediction precision, and shows good prediction performance in different clinical data. The detection method based on the fragment is expected to become a new tool for evaluating the assisted reproduction curative effect, the reproduction treatment scheme is further optimized, and the success rate is improved.

The present disclosure provides a sperm extractor, the sperm extractor may be a stimulation device, the sperm extractor including: a first massage member and a first driving mechanism, the first massage member including a first end, a second end opposite to the first end, and a lateral wall between the first end and the second end, the lateral wall surrounding and defining a first inserting space, the lateral wall including a first massage portion capable of massaging a glans of the penis received in the first inserting space; the first massage portion is driven to move by a motion of the connecting portion, the first massage portion is entirely deviating toward a first direction intersected with the inserting direction at a first moment and entirely deviating toward a second direction intersected with the inserting direction at a second moment, the first direction is different to the second direction.

The invention relates to the technical field of genetic relationship identification, in particular to a construction method, an identification method and application of a primary genetic relationship identification model based on ultralow deep sequencing. According to the identification model obtained by using the construction method of the genetic relationship identification model provided by the invention, the genetic relationship can be identified by adopting ultra-low depth sequencing (0.01 *), the genetic relationship can be determined according to the heterozygosity index between samples to be identified, the prediction accuracy is up to 99.9%, and the construction method is a first-grade genetic relationship detection technology with low cost and high accuracy; the method has the advantages of simple and convenient scheme, high accuracy, high credibility, high repeatability and high economic benefit, has multiple application scenes, and can advance an embryo paternity test window from antenatal / postpartum to before embryo implantation, thereby avoiding physical, mental and economic losses of a patient; ovum / sperm fertilization errors can be found in advance, non-parent embryo transplantation is avoided, and loss caused by embryo wrong transplantation is prevented.

The invention discloses a traditional Chinese medicine compound oral preparation for treating male oligoasthenospermia, and belongs to the technical field of traditional Chinese medicine preparations. The rhizoma polygonati processed with wine, the prepared rehmannia root and the like are used for nourishing the liver and kidney to consolidate congenital, the codonopsis pilosula, the astragalus membranaceus and the like are used for invigorating spleen and replenishing qi to complement postnatal, the fried cowherb seed is used for activating blood circulation, and the salted semen plantaginis is used for promoting diuresis, so that the problems of insufficient spermatogenesis and low vitality caused by deficiency of the spleen and the kidney are fundamentally solved. Experiments and clinical researches show that the preparation can significantly improve the sperm quantity, activity and activity rate of oligoasthenospermia model mice and patients, the clinical total effective rate reaches 90%, the pregnancy rate reaches 53.3%, and the curative effect is superior to that of Wuzi Yanzong pills in a control group. The action mechanism covers regulation of blood lipid metabolism, improvement of hypothalamus-pituitary-gonad axis functions, repair of testicular seminiferous tubule structural damage and reduction of lipid deposition, and meanwhile, oxidative stress and inflammatory injury are relieved and spermatogenic cell functions are protected by regulating and controlling a PPAR signal channel and inhibiting molecular pathways such as Cga / Sting mediated cell apoptosis.

A method for automated, artificial-intelligence-based egg identification using optical coherence tomography (OCT) includes positioning an OCT imaging system head in proximity to a biological sample containing an oocyte, wherein the OCT is operatively coupled to an artificial intelligence / machine learning system (AI / ML system) and an imaging system, wherein the imaging system includes a camera system, and a lighting system. The method includes creating at least one three-dimensional image of the oocyte using the OCT, AI / ML system, and imaging system. The method includes using the AI / ML system to analyze the three-dimensional image, wherein an analysis includes detection of a polar body's presence or absence based at least in part on planar views of the oocyte.

The invention discloses a spermatogenesis disorder sperm image recognition system based on deep learning, and relates to the technical field of sperm image recognition. The image recognition management center is in communication connection with an image acquisition module, a sperm positioning module, a feature extraction module, a motion feature analysis module, a sperm form classification module, a sperm state comprehensive recognition module and a result display feedback module, and all the modules are in electric signal connection. According to the method, morphology and motion features are learned and extracted from a large amount of sperm image data through a deep learning technology, the accuracy of sperm classification is improved, the time and errors of manual operation are remarkably reduced, meanwhile, fine differences of sperms can be accurately recognized, the diagnosis efficiency is greatly improved, and the method is suitable for popularization and application. Therefore, a doctor can obtain a more accurate diagnosis result in a shorter time, and a more timely treatment suggestion is provided for a patient.

The invention belongs to the technical field of breeding equipment, and particularly discloses a boar semen quality analysis method, device, equipment and medium, and the method comprises the following steps: 1) collecting a semen sample video, and uploading the video to a cloud; 2) performing frame extraction on the video to form an image, and performing pre-processing; 3) identifying information in the image; 4) tracking the sperms in the image according to the identification result; 5) analyzing and calculating a tracking trajectory result; and 6) scoring according to a calculation result, and outputting seminal fluid quality evaluation. Through a video monitoring function of a microscope camera, a semen video is recorded and uploaded to a cloud end (computer equipment does not need to be arranged through cloud end design), the video is subjected to picture interception and analysis through an AI algorithm, each sperm in the semen video is identified and tracked, a tracking result is analyzed and scored, and the sperm tracking accuracy is improved. And finally, giving out the activity score of the seminal fluid.

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention discloses an anti-freezing solution for improving the survival rate of sperms of Chinese prawns and a cryopreservation method of the anti-freezing solution, and belongs to the technical field of low-temperature biology and reproductive science. The invention provides an anti-freezing solution for cryopreservation of sperms of Chinese prawns, which comprises a basic solution and an anti-freezing agent, and the anti-freezing agent is dimethyl sulfoxide with the volume fraction of 10% or glycerol with the volume fraction of 10%; the basic liquid contains 23.69 g of sodium chloride, 1.1 g of potassium chloride, 14.8 g of magnesium chloride hexahydrate, 4.3 g of sodium sulfate, 0.23 g of sodium bicarbonate, 1.12 g of calcium chloride and distilled water with the constant volume of 1000 mL. According to the cryopreservation method provided by the invention, 10% dimethyl sulfoxide or 10% glycerol is adopted as an antifreeze agent, the sperm density is controlled to be 1 * 10 < 7 > / mL, and step-by-step cooling is carried out, so that the survival rate of unfrozen sperms reaches 90% or above. The long-term preservation effect of the Chinese prawn sperms is remarkably improved, and the method is suitable for germplasm resource protection and efficient breeding.

The invention belongs to the technical field of artificial insemination devices, and discloses an artificial insemination device for assisted reproductive technology, which comprises an outer catheter, and the left side of the outer surface of the outer catheter is provided with a first semen injection hole. By arranging the first semen injection hole, the movable plate, the inner guide pipe and the second semen injection hole, when the first semen injection hole and the second semen injection hole are staggered, the inner guide pipe can seal the first semen injection hole, so that secretions cannot enter the inner guide pipe through the first semen injection hole, and the semen injection efficiency is improved. When an operator inserts the outer catheter into the uterine cavity, the push rod can drive the movable plate to rotate by pushing the push rod, at the moment, the movable plate can drive the inner catheter to rotate, the first sperm injection hole and the second sperm injection hole coincide, and therefore the first sperm injection hole and the second sperm injection hole coincide with each other. Therefore, sperms can be injected into the uterine cavity through the first sperm injection hole and the second sperm injection hole.