1030 results about "Spermatid" patented technology

An optical pattern-driven light induced dielectrophoresis (DEP) apparatus and separation methods are described which provide for the manipulation of particles or cells and selection based on traits correlated with the DEP response. Embodiments of the apparatus use DEP electric field patterns in combination with microfluidic laminar flows to measure response, separate, segregate and extract particles from heterogeneous mixtures according to the relative response of the particles to one or more DEP fields without damaging living cells. The methods are particularly suited for selecting and extracting the best sperm and embryo candidates based on fitness for use with existing artificial reproduction procedures and excluding defective or non-viable gametes.

An intravaginal fertilization and culture container includes a main chamber and a microchamber. A culture medium, one or more oocytes and sperm are introduced through an access opening having an associated resealable closure device. The container is enveloped in a capsule of soft elastic material prior to lodgment in the posterior fornix. A loop on one capsule part adapts the length to anatomic variations. The microchamber has a channel which receives the catheter tip to facilitate embryo retrieval while eliminating risk of injury thereto. The microchamber permits embryos to be microscopically inspected in situ before transfer to the uterine cavity.

Invented is a method of inhibiting the activity / function of PI3 kinases using quinoline derivatives. Also invented is a method of treating one or more disease states selected from: autoimmune disorders, inflammatory diseases, cardiovascular diseases, neurodegenerative diseases, allergy, asthma, pancreatitis, multiorgan failure, kidney diseases, platelet aggregation, cancer, sperm motility, transplantation rejection, graft rejection and lung injuries by the administration of quinoline derivatives.

A practical method for freeze storage of fish sperm features that the diluent, antifreezing agent and container for the freeze storage of fish sperm chosen from many schemes, and a three-step freezing mode and a quick thawing method are designed.

An integrated microfluidic sperm isolation and oocyte insemination device provides the opportunity to perform in vitro insemination with motilityenhanced sperm samples and with minimal manipulation of fragile oocytes. Sperm sorting is performed in a common sort channel wherein more mobile sperm swim across the interface between co-laminar flows of semen and media fluid.

A pharmaceutical composition adapted for use as a spermicide, the composition comprising a gel-microemulsion comprising an oil-in-water microemulsion and a polymeric hydrogel. The gel-microemulsion can be used in a spermicidal method.Also, a gel-microemulsion pharmaceutical composition adapted for use as a formulation base for additional therapeutic agents. Examples of additional agents include, anti-microbial agents and spermicidal agents. Such gel-microemulsions with additional therapeutic agents can be used in methods for appropriate therapeutic treatment.Also, a gel-microemulsion pharmaceutical composition that is adapted for use as both a spermicide and formulation base for anti-microbial agents to provide a dual function contraceptive / anti-microbial formulation. Method of using such a composition as a dual function contraceptive / anti-microbial formulation are also included.

Sperm in semen are sorted by fluorescence-activated cell sorting into gender-enriched populations enriched in X-chromosome or Y-chromosome bearing sperm by use of a fluorescent quantitative DNA-binding vital stain.

A formulation for male contraception comprising a progestin possessing both estrogenic and androgenic properties is remarkably effective for spermatogenesis suppression in males. The progestin Norethisterone (NET), particularly its derivatives Norethisterone acetate and Norethisterone enanthate in sufficient doses induce oligozoospermia or azoospermia in males. Formulations further comprising an androgen, such as a testosterone derivative such as a testosterone ester, particularly testosterone undecanoate, are especially effective male contraceptive formulations.

The invention discloses a method for preparing male sterile bodies of argopecten irradians. Argopecten purpuratus which is homogeneous with the argopecten irradians, has the same chromosome number and similar gamete form and size as the argopecten irradians and is androgynous is selected to hybridize with the argopecten irradians so as to prepare male sterile bodies. The testes of the male sterile bodies are gradually substituted by ovaries together with the development of gonads; and when the gonads are mature, the whole gonads are orange red special in female, can only produce ova and cannot produce sperms. The method disclosed by the invention has the advantages of high proportion of sterile bodies, stable technical effect, relative simpleness, easiness for popularization.

A seat (chair, stool, etc.) has a seat base with a contoured front that is recessed in the middle. The recess extends towards a seating center and / or in a downward direction, creating an open area for seated person. The open area is located under the body part around the testes of a seated male person so that the dispersion of body heat from that area via air circulation will not be blocked by the seat base, and at the same time, the pressure between the testes area and the seat base will be reduced or eliminated, thereby improving the physiological condition for sperm, and in turn, may increase sperm activity and may reduce the possibility of infertility.

The present invention relates to a method and device for male contraception. In one aspect, the method and device provide temporal and reversible infertility of mammal males without suppressing spermatogenesis. In an aspect, a method of contraception for use with reproductive cells that pass through a tube in a body is described in which energy is applied through the tube wall to some of the reproductive cells that pass through the tube to reduce fertilization ability of the some of the reproductive cells. In another aspect, the device is adapted for use with reproductive cells that pass through a tube in a body, and includes an energy transmitter that delivers energy to the reproductive cells that pass through the tube in the body.

The present invention provides methods for preparing cells with highly condensed chromosomes, such as sperm, and methods for detecting and quantifying specific cellular target molecules in intact cells. Specifically, methods are provided for detecting chromosomes and chromosomal abnormalities, including aneuploidy, in intact cells using fluorescence in situ hybridization of cells in suspension, such as sperm cells.

This invention describes unique patterns of distribution of ganglioside GM1 in non-capacitated sperm and demonstrates that the pattern of distribution of GM1 undergoes changes that can be correlated with the process of capacitation and / or with acrosomal exocytosis. Accordingly, the present invention discloses a method for determining the ability of sperm to respond to capacitation and / or acrosomal exocytosis stimuli. The method comprises determination of distribution pattern for GM1. The method can be used for both diagnostic and predictive purposes when assessing male reproductive fitness, and can also be used to assess the effects of any agent or environment on sperm including cryoprotective agents and protocols, and contraceptive agents.

The invention relates to a method for producing male fish, wherein it comprises that: artificially inducing; artificially inducing the female and male fishes; artificial insemination; inverting hormone; feeding seed with Artemia which contains hormone; converting male into biological female; the female grows. The invention comprises sperm inactivation and female diploid generation, to generate ready male fish and normal female fish; crosses the male fish and female fish to obtain the full male fish; inverts the male fish into biological female fish to cross with male fish, to obtain the full super male fish.

Lubricant compositions and methods are disclosed for aiding and enhancing massage or sexual activity, treating and preventing sexually transmitted diseases, and preventing pregnancy by administering a colloidal metal containing composition. Colloidal metal containing compositions that include metal flakes, sperm-function inhibitors, antimicrobial agents, analgesics, plant extracts, anti-oxidizing agents, and anti-inflammatory agents are also provided and may advantageously be used to in the methods of the invention.

The invention discloses a special feed additive for conditioning the breeding performance of artificially inseminated breeder cocks and application thereof. The special feed additive for conditioning the breeding performance of artificially inseminated breeder cocks is prepared from a composite traditional Chinese herbal additive and a nutritional feed additive according to a weight ratio of 1:1, wherein the composite traditional Chinese herbal additive comprises 7-10 percent of epimedium, 7-10 percent of actinolite, 7-10 percent of yam, 7-10 percent of prepared rhizome of rehmanniat, 5-8 percent of medlar, 5-8 percent of cistanche salsa, 5-8 percent of dodder, 5-8 percent of raspberry, 5-8 percent of plantain seeds, 5-8 percent of japanese teasel, 5-8 percent of liriope, 3-4 percent of Dangshen, 3-4 percent of morinda officinalis, 3-4 percent of astragalus, 3-4 percent of angelica sinensis, 3-4 percent of atractylodes and 3-4 percent of glycyrrhiza, and the percent sum of the raw materials is 100 percent. The nutritional feed additive comprises 35-45 percent of alfalfa meal, 20-25 percent of folium cortex eucommiae powder, 20-25 percent of mulberry leaves powder, 4-9 percent of bone meal, 5-10 percent of saleratus and 0.5-1 percent of trace elements and composite vitamin additive, and the percent sum of raw materials is 100 percent. After the special feed additive is applied, the average ejaculate volume of breeder cocks is improved by 17.6 percent, the sperm activity is improved by 16.1 percent, and the sperm density is improved by 14.2 percent.

Described herein is a composition that comprises a cryoprotectant; a membrane protectant that stabilizes or assists in stabilization of membranes of sperm; and a free radical scavenger (e.g., a reducing agent, an antioxidant).

The invention relates to cell culture media more particularly to fertilisation media, to making and using transgenes, to providing sperm cells for fertilisation particularly in applications such as sperm-mediated gene transfer and to using sperm cells for generating transgenic animals.

The invention discloses a novel frozen semen diluent used for improving the freeze-thaw quality and fertilization rate of frozen bovine semen and a preparation method thereof. Every 100ml of the prepared frozen semen diluent contains 0.9-1.2g of sodium citrate, 3-5g of sucrose, 18-22ml of yelk, 4-7ml of glycerin, 6-9mg of vitamin E, 500-700mg of vitamin C, 35mg of spectinomycin and 80 thousand units of gentamicin. The invention adds VE and VC into the semen diluent for the first time, so as to increase the motility rate and acrosome integrity of the frozen sperm as well as the activity of antioxidant enzyme in seminal plasma, thus improving the quality and fertilization rate of the freeze-thaw sperm.

The invention relates to a method for culturing an in-vitro fertilization embryo of an oocyte collected from a living cow or in vitro. The method comprises the following steps: collection and in-vitromaturation of the oocyte, wherein the oocyte can be collected in vitro or from the living cow; in-vitro fertilization, wherein mature COCs is cultured in a fertilization culture solution and then themature COCs and sperm suspension are subjected to co-incubation culture; and in-vitro culture of the embryo and cryopreservation in liquid nitrogen. The method has the excellent technical effect as shown in specification.

The invention belongs to the technical field of livestock molecular marker preparation, and concretely relates to a molecular marker related to a sperm activity character of a boar and application. The molecular marker is screened from a PRM2 gene; a nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. An allelic mutation R exists in nucleotide in position 169 of a sequence table, R is G or A, and the mutation causes the polymorphism of NaeI-RFLP. The invention further discloses a primer pair for amplifying PRM2 gene SNP (Single Nucleotide Polymorphism), and the primer pair is also the primer pair for detecting the screened molecular marker provided by the invention. The invention builds a method for detecting the polymorphisms of the sperm activity character of the boar. Association analytic application shows that the molecular marker provided by the invention can be used as the marker for assistant selection of the sperm activity character of the boar.

The invention relates to Chinese herbal medicine feed additive for stud rams, which mainly solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art. The Chinese herbal medicine feed additive is prepared by crashing and mixing epimedium, actinolite, Chinese dodder seed, raspberry, Chinese magnoliavine fruit, astragalus root and dangshen and can be added into feed for usage. The invention improves ram sexual appetite, prompts spermatogenesis, increases sperm motility, effectively improves reproductive performance, solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art according to spermatogenesis principle.

The invention discloses a health-care food for males and a preparation method thereof. The health-care food is prepared according to a traditional Chinese medicine theory and the respective health-care efficacies and characteristics of maca, cistanche and liquorice; maca has the efficacies of resisting fatigues, improving sexual functions, increasing the number of sperms, improving the quality of the sperms, reducing benign prostatic hyperplasia, enhancing fertility and the like; traditional Chinese medicine cistanche has the efficacies of benefiting essence and blood, tonifying the kidney and reinforcing yang as well as lubricating intestines and smoothing bowels and can have health-care effects on kidney deficiency and impotence, spermatorrhea and premature ejaculation, coldness and pain of a waist and knees and the like; liquorice is a tonifying Chinese herbal medicine and can coordinate drug properties as well as tonify spleen and benefit qi. By virtue of a nanotechnology, the bioavailability of bioactive molecules in maca, cistanche and liquorice is improved; by virtue of fine processing, maca, cistanche and liquorice are prepared into the health-care food for males; the health-care food is safe and effective, nontoxic and harmless, and has synergistic effects on improvement of sexual function, enhancement of fertility, regulation of internal secretion, reduction of prostate diseases and improvement of the immunologic function of an organism.

The invention discloses a cryopreservation method for sperms of ablen, which comprises the following steps of: sampling the sperms, performing microscopic examination, mixing the sperms and an antifreeze agent, and freezing. The cryopreservation method is simple and feasible, has obvious effect and good cryopreservation effect. If the sperms of the ablen most important in Chinese aquiculture are preserved by the method, the activity of the unfrozen sperms is more than 90 percent, and the bottleneck problems of insufficient male fish and asynchronous sexual maturity of female and male parent fish in the ablen culture are effectively solved.

The presently-disclosed subject matter relates to antibodies, compositions, and methods for inhibiting and treating pathogen infection and providing contraception. In particular, the presently-disclosed subject matter relates to inhibiting and treating pathogen infection and providing contraception in a subject using compositions and antibodies capable of trapping pathogens or sperm in mucus, thereby inhibiting transport of pathogens or sperm across mucus secretions. The subject matter further relates to methods for monitoring the effectiveness of vaccines by detecting antibodies capable of trapping pathogens in mucus.

The invention which belongs to the medical field of genetic engineering and reproduction discloses a seminal plasma miRNA marker associated with human non-obstructive azoospermia and an application thereof. The maker is selected from several of hsa-miR-141, hsa-miR-193a-5p, hsa-miR-590-5p and hsa-miR-7-1*. The maker which has specificities and sensitivities to the non-obstructive azoospermia can be used for the preparation of a reagent for non-obstructive azoospermia diagnosis or monitoring, so invasive diagnosis is avoided, repeated detection is realized, and the dynamic monitoring of the obstacle degree of sperm generation can be easily achieved.

The invention discloses a boar semen refrigerating method and dedicated refrigerating fluid thereof. The method comprises the following steps: (1) diluting dense boar semen according to a volume ratio of 1:(0.5-3), stewing at 26-30 DEG C for 25-40 minutes, and centrifuging 800g of boar semen for 10 minutes to obtain sperm precipitation; (2) adding refrigerating fluid I into the sperm precipitation according to a volume ratio of 1: (1-1.5), stewing at 17-20 DEG C for 1-2 hours, and stewing at 4-7 DEG C for 2 hours to obtain a system I; (3) adding refrigerating fluid II containing glycerin into the system I to obtain a system II with final glycerin concentration of 3%; (4) stewing the system II at 4 DEG C for 1-2 hours, and adding caffeine to obtain a system III with final caffeine concentration of 0.2g / L; and (5) stewing the system III at 4 DEG C for 0.5-2 hours, dropwise refrigerating at -120 DEG C, stewing and balancing for 5-10 minutes, and submerging the system III in liquid nitrogen for cryopreservation. After the boar semen refrigerated by the method is thawed, the sperm survival rate of the boar semen is higher than 0.45, and the in vitro survival time of the boar semen is longer than 6 hours, thereby reaching the production application level. The boar semen refrigerating method disclosed by the invention is used for providing scientific basis for the application and popularization of the refrigerated boar semen in production, and providing a technical method and standard for boar semen refrigerating.

The invention relates to a sperm and ovum health beverage which comprises fulvic acid, gene vitality element, plant extract, fungus polysaccharide, vitamin, hydroxycitric acid (HCA), soluble dietary fiber, L-arginine, L-carnitine, zinc gluconate, calcium lactate, sweetening agent, milk, coffee and purified water. The invention has simple technology, low cost and complete product nutrition and contains rich natural polypeptide, polysaccharide, amino acid and vitamin and various trace elements. All components interact to promote blood circulation and sex hormone secretion so as to achieve the functions of promoting the growth of sperms and ovums, improving the quality and the vitality of the sperms and the ovums and improving the sexual function. The sperm and ovum health beverage has the good improving effect on ED symptoms including renal dysfunction, working strain, heavy spirit pressure, bradypsychia, palpitation, stomachache, weak waist and knee, sexual dysfunction, and the like. As a pure natural preparation, the sperm and ovum health beverage has no poison and side effect and adverse reaction.

The present invention is a sperm collector with squeezing function. It includes a soft rubber body provided with a penis insertion passage and a container body provided with at least a top end cover and receives the soft rubber body. When the top end cover and the container body are engaged, the soft rubber body is confined within a cavity formed by the top end cover and the container body. When the top end cover is disengaged, a front end portion of the soft rubber body protrudes out of an opening of the container body. A pressing means is provided at a middle lower portion of the container body. When using the present invention, the user could press the pressing means to squeeze the penis insertion passage of the sperm collector. Accordingly, stimulation to penis is increased and better effect of sperm collection is achieved. In particular, since the present invention is also provided with a heating rod, lubricant pockets and electrical vibrators, which enable the user to reach a climax as quick as possible, thereby further enhancing the effect of sperm collection.