426 results about "Spermatid" patented technology

The invention relates to a sperm cell analysis and diagnosis system based on a multi-modal large language model, which comprises a multi-modal data co-processing unit, a cross-modal semantic alignment module, a dynamic diagnosis decision engine and a self-adaptive evolution system, the four-dimensional data processing module is used for synchronously processing microscopic images, motion trail videos, biochemical detection data and four-dimensional input data of medical record texts and comprises a feature selector based on a gating attention mechanism. According to the sperm cell analysis and diagnosis system based on the multi-modal large language model, quantitative analysis of sperm movement chaos features is realized for the first time, a nonlinear dynamic evaluation standard is established, a cross-modal knowledge distillation and meta-learning migration framework is developed, a data annotation dependence bottleneck is broken through, and an interpretable clinical decision support system is constructed; dynamic updating and probabilistic suggestion of diagnosis rules are achieved, semantic analysis of single-cell multi-omics data is achieved, and molecular mechanism research results are converted into clinically available knowledge.

The invention provides a method for overspeed preparation of gene edited goldfish. The method comprises the following steps: S1, obtaining gobiocypris rarus without primordial germ cells as receptor fish; s2, designing a target spot for the goldfish amh gene, introducing a Cas9 / gRNA complex into a goldfish fertilized egg, performing sequencing verification to obtain a mutant individual, and feeding the mutant individual as a donor fish; s3, taking gonads of donor fish, cutting into pieces, performing enzymolysis digestion, filtering, and performing density gradient centrifugation to enrich donor germline stem cells; s4, transplanting the germline stem cells of the donor fish into the receptor fish body, and feeding to obtain the gene edited goldfish sperms and ova. S5, hybridizing and feeding goldfish amh mutant sperms and ova produced by gobiocypris rarus to obtain gene edited goldfish individuals. According to the invention, gobiocypris gobiocypris is used as a receptor, the cross-subfamily gene edited goldfish is used as a donor, and an efficient, stable and easy-to-popularize method for preparing the gene edited goldfish is provided by utilizing a belly borrowing reproduction technology.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

The application discloses PRR30 gene SNP and a haplotype combination thereof in identifying bull semen quality, and belongs to the technical field of molecular genetics. Eight SNP sites are identified, wherein SNP7: g.794 C>A or SNP8: g.2247 G>T can be used for identifying the high and low of bull semen collection amount, SNP2: g.-2151 C>T can be used for identifying the high and low of bull sperm abnormality rate, and the haplotype combination of SNP1-SNP8 is that the bull with H1H11 has higher semen collection amount and lower sperm abnormality rate, and can be further used for identifying bull semen quality, thereby helping staff to evaluate bull breeding performance in an early stage, to carry out superior gene bull screening, to promote the cultivation of superior bull, to shorten the screening period and to improve the breeding efficiency.

This invention relates to the field of beauty and skincare, and discloses an automated system and method for detecting semen quality, comprising: a morphological defect rate calculation module for calculating the morphological defect rate corresponding to a target semen sample; a dysfunctional sperm identification module for identifying dysfunctional sperm in the target semen sample; a reproductive function scoring module for evaluating the reproductive function score corresponding to the target semen sample; a dynamic motility potential scoring module for configuring a suitable culture medium for the target semen sample, culturing the target semen sample in the suitable culture medium, recording the sperm viability baseline parameters during the culture process, calculating the sperm survival rate of the target semen sample, and evaluating the dynamic motility potential score corresponding to the target semen sample; and a semen quality analysis module for generating a semen quality analysis report corresponding to the target semen sample. This invention can improve the accuracy of semen quality detection.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The application belongs to the field of fish development, and particularly relates to a molecular marker for identifying Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring, a primer set thereof, a kit and application. The primer set for identifying the molecular marker of the Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring comprises primers F and R, the nucleotide sequence of the primer F is shown as SEQ ID NO. 1, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 2. Based on genomic data analysis, the application develops the molecular marker F / R which can effectively identify the Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring in the juvenile stage. The method comprises extracting genomic DNA of parents and offspring, PCR amplification and agarose gel electrophoresis detection, and finally distinguishing the gynogenesis individuals and hybrid individuals by whether the Nibea albiflora parent marker is contained in the electrophoresis band.

The invention discloses a traditional Chinese medicine composition for preventing and treating light and moderate varicocele and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 12 to 30 parts of radix paeoniae rubra, 9 to 24 parts of peach kernel, 9 to 24 parts of spina gleditsiae, 9 to 24 parts of radix cyathulae, 6 to 18 parts of cassia twig, 9 to 24 parts of fructus forsythiae, 6 to 18 parts of thunberg fritillary bulb, 6 to 18 parts of rhodiola rosea, 6 to 18 parts of semen aesculi and 3 to 15 parts of raw liquorice. After the traditional Chinese medicine composition provided by the invention is adopted for treatment, the spermatovenous reflux time is remarkably shortened, the semen quality including semen volume, sperm density and sperm motility is remarkably improved, the total effective rate of treatment is higher than that of other prescriptions, the living quality of a patient is remarkably improved, the medicine safety is high, and the varicocele can be effectively treated.

An apparatus for diagnosing an embryo implantation potential using an artificial intelligence-based diagnostic model according to the present disclosure includes a memory storing a diagnostic model trained to determine an implantation potential index for an embryo based on an image generated by photographing one or more embryos formed by fertilization of a sperm and an oocyte and before implantation, and a processor for inputting a diagnostic image generated by photographing one or more first embryos, which are determination targets of the implantation potential index, into the diagnostic model as input data, and receiving implantation potential index data, which is a result of determining the implantation potential index for the first embryo, as output data from the diagnostic model.

The invention discloses an efficient extraction method of blue fox sperm DNA, and belongs to the technical field of molecular biology. Aiming at the structural characteristics that chromatin of the blue fox sperms is highly condensed, histone is replaced by protamine, and a stable nucleoprotein complex is formed through an intermolecular disulfide bond, the invention provides an efficient DNA extraction method suitable for the blue fox sperms. According to the method, based on the synergistic effect of three chemical reagents, namely SDS, PK and DTT, full lysis of the sperm cells of the blue foxes is achieved, and then efficient enrichment of sperm DNA of the blue foxes can be achieved by combining a conventional DNA extraction method. According to the efficient extraction method of the blue fox sperm DNA provided by the invention, the yield and integrity of the blue fox sperm DNA are remarkably improved, and the limitation that sperm chromatin is difficult to effectively lyse by a conventional method is overcome; the method provides reliable technical support for research on genetic diversity evaluation, population management, molecular breeding and the like of the blue foxes, and has the potential of popularization and application in other high-condensation sperm species.

The invention belongs to the technical field of artificial insemination of bumblebees, and particularly relates to a male bumblebee sperm collecting device. The male bee seminal vesicle extraction platform is arranged on the base, the male bee seminal vesicle extraction platform is used for clamping the male bee seminal vesicle glass sheet, the male bee seminal vesicle glass sheet is used for placing a male bee seminal vesicle, and the male bee seminal vesicle extraction platform drives the male bee seminal vesicle glass sheet to move in the horizontal direction of the base; the insemination needle fixator is vertically arranged on the base in a lifting mode and used for fixing a glass needle, and the glass needle faces the male bee seminal vesicle glass sheet. Through the device, the extraction efficiency can be remarkably improved, so that the sperm exposure time is effectively shortened, and relatively high vitality is kept.

The invention discloses a method for predicting the sperm motility of a breeding bull based on a host SNP-rumen microorganism-metabolite interaction axis, and the sperm motility of the breeding bull can be obtained by detecting the haplotype condition of SNP1-SNP3 and / or SNP4-SNP6 sites of a breeding bull genome. The method breaks through the limitations of strong dependency on phenotype endpoint evaluation and prediction lag in the current bull seed selection process, and realizes forward accurate prediction and early evaluation of the semen quality of the breeding bull.

The invention provides an application of methylsulfonylmethane in preparation of a product for treating asthenospermia. The research of the invention finds that the sulfhydrylation modification level of ATP synthetase can be effectively changed by using methylsulfonylmethane as an active component, so that the intracellular energy metabolism state of sperms is regulated and controlled, and the motility of the sperms is further changed.

The application relates to the technical field of biological medicine, and discloses a drug composition containing nuclear autoantigen sperm protein for promoting myocardial regeneration and repair and application, which comprises an NASP gene; the nucleotide sequence of the NASP gene is shown as SEQ ID NO:1, and the amino acid sequence encoded by the NASP gene is shown as SEQ ID NO:2. The drug composition can promote the number of myocardial cells, repair the necrotic myocardium, and thus improve acute myocardial infarction at the gene level.

The invention relates to the technical field of semen preservative preparation, in particular to a diluent preparation capable of prolonging the normal-temperature survival time of boar semen and a preparation method of the diluent preparation. Glucose, L-glutamine, an antioxidant and polysaccharide components are scientifically proportioned, and an innovative polyethyleneimine-polysaccharide copolymer is combined, so that the normal-temperature survival time of boar semen is prolonged; the dual effects of sperm metabolism regulation and cell membrane protection are realized. The polyethyleneimine-polysaccharide copolymer enhances the biocompatibility and protective ability of the diluent through stable cross-linking of amino and carboxyl, and effectively relieves the problem of sudden change of osmotic pressure and acidity of the traditional preservative. In the formula, antioxidant components, such as reduced glutathione, vitamin E and coenzyme Q10, synergistically reduce oxidative stress and delay sperm senescence. According to the whole formula, proper pH and osmotic pressure are guaranteed, the sperm motility and survival time are remarkably prolonged, the requirement of pig industry for long-term preservation of sperm is met, and the feed additive has remarkable application value and popularization potential.

The present disclosure describes proteins that specifically bind to human sperm protein 17 (Sp17) with nanomolar affinity and high specificity. These proteins include recombinant human anti-Sp17 IgG, which are suitable for use as therapeutic antibodies to treat cancers that express Sp17 ectopic. Other Sp17 binding proteins, including antibody fragments, antibody conjugates, and fusion proteins, are also described.

The invention provides an application of ergothioneine as a testis oxidative stress regulator. The invention provides a multi-target intervention method based on ergothioneine, which systematically improves sperm quality reduction caused by obesity by cooperatively regulating key links such as oxidative stress level, hormone secretion state and mitochondrial function. According to the invention, a safe and effective intervention effect with a clear action mechanism can be realized, and a new technical approach is provided for the treatment of obesity-related male reproductive dysfunction. The invention also provides application of the ergothioneine as the testicular steroid generation accelerant.

The sperm optimal tube comprises an upper tube, a lower tube and an inner tube, the upper tube and the inner tube are communicated, the upper tube is detachably connected with the lower tube, when the upper tube is connected with the lower tube, the lower tube is located outside the inner tube, one end of the inner tube is communicated with the upper tube, and the other end is provided with an opening allowing sperm to pass through, and a switch valve is arranged on the opening, the switch valve can be opened under the action of centrifugal force and closed after the centrifugal force stops. The device can effectively separate normal and active sperm, and especially because the switch valve is arranged, the sperm is allowed to flow out under the action of centrifugal force, and the switch valve is closed to seal the inner tube when the centrifugal force is eliminated, so that the abnormal sperm, the inactivated sperm and other cell components in the semen in the inner tube are prevented from entering the lower tube, and the complete separation of sperm with different activities is realized; the design of the inner tube, the upper tube and the lower tube facilitates the disassembly of the outer tube, the semen is more convenient to take out, and the mutual interference is avoided.

The invention discloses a sperm genome methylation detection method for safety evaluation of biological breeding crops by using primates and application, and relates to the technical field of safety evaluation of crops, the sperm genome methylation detection method comprises the following steps: dividing non-human primates into three groups, collecting sperms after long-term feeding, and extracting DNA (Deoxyribose Nucleic Acid); carrying out whole genome sequencing and quality control after bisulfite treatment; the epigenetic safety of crops is comprehensively evaluated by analyzing the methylation level of a whole genome and a functional region and functional enrichment of a differential methylation region and related genes thereof; according to the sperm genome methylation detection method for evaluating the safety of the biologically bred crops by utilizing the primates and the application, by utilizing a high-resolution WGBS technology, subtle epigenetic changes which are difficult to find by traditional toxicology can be detected, and the sperm genome methylation detection method has important significance in cross-generation reproduction effect evaluation, and has a wide application prospect. A food safety evaluation system can be perfected, and a more scientific and reliable safety interpretation basis can be established.

The invention discloses a sperm sample layering treatment centrifuge tube support device. The device is composed of a machine body, a test tube placing rack, a test tube locking mechanism and a centrifuge cover. The test tube placing rack is arranged in the inner cavity of the machine body and is provided with a test tube placing table for placing a centrifugal test tube filled with a sperm sample. The test tube locking mechanism is used for stably fixing the centrifugal test tube on the test tube placing table through components such as a central block, a Z-shaped connecting rod, a silica gel lining ring and a hoop. The centrifugal machine cover can be closed to shield the machine body, and an ultraviolet disinfection lamp is arranged on the inner side of the centrifugal machine cover and can disinfect the inside of the device. During use, after sample test tubes are placed and fixed, the machine cover is closed, a power supply is connected to set parameters, and the device is started to enable the motor to drive the test tubes to rotate at high speed to realize sample layering. After centrifugation is finished, the cover is opened, the test tube is taken out, and then the ultraviolet disinfection lamp can be turned on to disinfect the inside of the device to guarantee cleanness and sanitation.

This image is an information display image used for observing sperm in assisted reproductive technology. This image includes an object (information display section) that is displayed according to the position of the sperm and displays information about the sperm, and an object (display information selection section) for selecting information about the sperm to be displayed by the information display section. The information display section displays information about the state of the sperm, for example, using color or brightness. The display information selection section is an operation section that enables selection of information about the sperm to be displayed by the information display section. Image 1 showing the changed state is an image showing the state in which the information displayed by the information display section in the image diagram has changed over time, etc. Image 2 showing the changed state shows that it is also possible to prevent the information display section from being displayed by selecting the display information selection section.

The invention belongs to the technical field of biology, and particularly discloses a chicken semen cryopreservation solution and a preparation method thereof. The chicken semen cryopreservation liquid comprises a cryoprotection liquid I and a cryoprotection liquid II. The freezing protection liquid I is prepared from the following components: glucose, sodium glutamate, potassium acetate, dipotassium phosphate, magnesium acetate, N, N-bis (2-ethoxyl)-2-aminoethanesulfonic acid and an antibiotic solution. And the freezing protection liquid II comprises dimethylformamide and nano zinc oxide. The invention discloses a chicken semen cryopreservation solution and a preparation method thereof, by applying the chicken semen cryopreservation solution, the fertilization and incubation performance of frozen semen is remarkably improved, centrifugation-free and direct semen deposition of the frozen semen are realized, and physical damage and activity reduction caused by the centrifugation process to sperms are avoided.

The invention relates to the technical field of image processing and reproductive hospital inspection, and provides a sperm movement tracking and quality analysis method based on deep learning and optical flow fusion, which comprises the following steps of: identifying sperms through a target detection model, acquiring a detection frame, and screening to obtain detection points; predicting the predicted position of the sperm in the current frame by using a sparse optical flow method based on the historical trajectory, performing data association on the predicted position and a detection point, establishing a matching relationship, updating the trajectory according to the matching relationship, establishing a new trajectory or terminating a lost trajectory, calculating kinematics parameters and sperm concentration based on the complete motion trajectory, and completing activity grading. According to the invention, through fusion of deep learning detection and optical flow prediction, high-precision identification under a complex background and stable tracking under a high-density cross scene are realized, the identity exchange rate is significantly reduced, and the shielding robustness is enhanced; and meanwhile, full-process automation from video input to clinical report is realized, and the accuracy, repeatability and clinical credibility of an analysis result are improved.

The application belongs to the technical field of fish variety improvement, and discloses a method for creating gynogenetic grass carp with rapid growth. The method comprises the following steps: screening sexually mature male mirror carp and sexually mature female grass carp with obvious growth advantages and stress resistance, collecting sperm of the mirror carp, diluting the sperm with Hank's solution, inactivating the sperm by ultraviolet irradiation, mixing the obtained inactivated sperm with middle-stage eggs of the grass carp, and then performing cold shock doubling treatment, and adding normal temperature water in batches for hatching to obtain gynogenetic grass carp. The gynogenetic grass carp induced by the mirror carp sperm performs excellently in growth speed and disease resistance, which provides a new method and idea for inheritance improvement of the grass carp, provides more possibilities for screening of excellent varieties, and brings greater economic benefits to the grass carp breeding industry.

This invention discloses a method, system, device, medium, and application for constructing a diagnostic model for male infertility, belonging to the fields of male infertility diagnosis, drug screening, and assisted reproductive technology. In this invention, by simultaneously and quantitatively analyzing the pH value, mitochondrial G-quadruplex structure level, and β-galactosidase activity within a single sperm cell, and integrating routine clinical sperm testing parameters and age, a high-precision diagnostic model for male infertility and a drug screening system are established, providing a reference for assisted reproduction to meet practical needs.

The invention relates to the technical field of traditional Chinese medicine, in particular to a medicine for improving sperm quality and application thereof.The medicine is reasonably combined according to etiology and pathogenesis of male infertility with abnormal sperm quality, prepared rehmannia root and poria cocos serve as monarch medicine, cistanche, astragalus membranaceus and ginseng serve as ministerial medicine, maca serves as adjuvant medicine, kidney tonifying is assisted, and the effect of improving the sperm quality is achieved. The traditional Chinese medicine composition has the effects of tonifying kidney, replenishing essence, promoting diuresis, dissolving turbidity and treating spleen and kidney through compatibility and synergism of all the medicines, improves abnormal sperm quality, improves sperm quality, effectively treats male infertility and can take both symptoms and root causes into consideration.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

This invention discloses a pharmaceutical composition for treating asthenospermia by activating the Nrf2 / HO-1 / GPx4 pathway and its application. The pharmaceutical composition comprises taurine and geranium as active ingredients, wherein the mass ratio of taurine to geranium is 3-5:1. The pharmaceutical composition of this invention can synergistically activate the Nrf2 / HO-1 / GPx4 antioxidant signaling pathway, upregulate the expression of downstream antioxidant enzymes GPx4 and HO-1, inhibit lipid peroxidation, protect sperm mitochondrial function, and simultaneously improve the microenvironmental metabolic profile of the epididymis—the site of sperm maturation—thereby enhancing sperm motility. The pharmaceutical composition provided by this invention can be used to prepare a treatment for asthenospermia, particularly oxidative stress-induced asthenospermia.

This invention discloses a sperm quality detection sensor and its preparation method, belonging to the field of detection and sensing technology. It includes an electrode with an HA functional layer modified on its surface, the HA functional layer having amino active sites; a redox probe that generates an electrical signal with the electrode; and a conductive layer disposed between the electrode and the redox probe for conduction between them. This application specifically binds the HA functional layer to receptors on the surface of healthy sperm in the test semen and employs microcurrent signal processing technology. The specific binding of the HA functional layer to the receptors affects the current between the electrode and the redox probe. The change in current of the sperm quality detection sensor characterizes the number of healthy sperm in the test semen. This sperm quality detection sensor overcomes the limitations of morphological interpretation in traditional detection technologies, significantly reducing the size of sperm detection equipment and improving the accuracy of sperm quality detection.