1187 results about "Vesicle" patented technology

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention relates to the technical field of pharmaceutical preparations, and discloses a composition with liver protection and antioxidation effects and a preparation method thereof.The composition is prepared from galactosamine modified astragalus membranaceus exosome, schisandra chinensis nanocrystals, liquorice-chitosan nanoparticles, lactobacillus acidophilus freeze-dried powder, beta-(1, 3) / (1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate) sourced from saccharomyces cerevisiae, and beta-(1, 3, 4-tetramethyl-1, 3-pentanediol monoisobutyrate). And (6) a dextran, grape exosome-curcumin hybrid vesicle freeze-dried powder, a curcumin-mitochondrial targeting peptide compound, a resveratrol-hydroxypropyl-beta-cyclodextrin inclusion compound and reduced panthenol. According to the liver-protecting and anti-oxidation composition disclosed by the invention, multi-dimensional intervention on liver protection is realized through an elaborately designed ternary system. The system is composed of a traditional Chinese medicine extract, a microorganism-plant exosome combination and a high-bioavailability antioxidant, all the parts have a synergistic effect, and the liver protection effect is remarkably improved.

The invention discloses a method for extracting extracellular vesicles from dried Sanguisorba officinalis rhizomes and application of the extracellular vesicles, and relates to the technical field of molecular biology and biomedicine.The method comprises the following steps that S1, rehydration is conducted, specifically, the dried Sanguisorba officinalis rhizomes are subjected to liquid nitrogen quick freezing and then ground into powder at the low temperature, the powder and hydration liquid are mixed according to the proportion of 1: 10 (w / v), the mixture is slowly stirred for 2-4 h at the temperature of 4 DEG C, and the extracellular vesicles are obtained; removing large-particle impurities by using sterile gauze or a filter screen, and collecting filtrate; according to the method, through the synergistic effect of multiple enzymolysis and in combination with cellulase, pectinase and the like, the degradation efficiency is remarkably improved, meanwhile, EDTA is chelated with metal ions, and the enzymolysis effect is enhanced. Short-time alternating ultraviolet light is introduced to activate'dormant 'vesicles, so that the yield and the biological activity are improved. Secondly, multiple protection mechanisms, such as vitamin C, EDTA (Ethylene Diamine Tetraacetic Acid), cane sugar, BSA (Bovine Serum Albumin) and an ultraviolet protective agent are adopted, so that the integrity and the biological activity of the vesicles are ensured.

The invention relates to a universal nano biological vesicle preparation method. The preparation method comprises the following steps: carrying out crude extraction on cells from bacteria, fungi, plants or animals to obtain a crude extracting solution; the crude extracting solution is placed in a low-temperature and high-pressure homogenizer to be subjected to low-temperature and high-pressure homogenizing treatment, then the nano vesicles are obtained, the low-temperature and high-pressure homogenizer comprises a high-pressure cavity, a homogenizing valve, a working cavity, a conveying system and a temperature control system, a slit is formed in the homogenizing valve, and the crude extracting solution located in the high-pressure cavity enters the working cavity through the slit of the homogenizing valve, the pressure intensity of the high-pressure cavity is 300-3000 bar, and the working temperature of the high-pressure homogenizer is 0-10 DEG C. The activation energy is reduced by controlling high-pressure and low-temperature environments, so that the original cell membrane structure is broken, the cell wall structure is destroyed, and phospholipid bimolecules are driven to aggregate and self-assemble to form the vesicles. For biological membranes derived from bacteria, fungi, plants and animals, the vesicles can be prepared by the preparation method disclosed by the invention, and the method has universality.

The invention discloses application of camellia nitidissima exosome-like nano-vesicles in preparation of antitumor drugs, and belongs to the technical field of biological medicines. The exosome-like nano-vesicles are extracted from golden camellia for the first time, and the exosome-like nano-vesicles are proved to have remarkable anti-tumor activity.

The invention relates to polygonum multiflorum exosome-like vesicles and application thereof, the polygonum multiflorum exosome-like vesicles are applied to preparation of antioxidant products, anti-inflammatory products and anti-ultraviolet products, the antioxidant products are products for removing one or more of DPPH free radicals, ABTS free radicals and hydroxyl free radicals, the anti-inflammatory products are products for inhibiting hyaluronidase, and the anti-ultraviolet products are products for removing one or more of DPPH free radicals, ABTS free radicals and hydroxyl free radicals. And the anti-ultraviolet product is a product capable of absorbing UVB. Compared with the prior art, the polygonum multiflorum exosome-like vesicles prepared by the method disclosed by the invention are low in cost and large in treatment capacity, and the obtained polygonum multiflorum exosome-like vesicles have excellent oxidation resistance, inflammation resistance and ultraviolet resistance.

The invention discloses aloe exosome-like nano-vesicles capable of delaying skin photoaging and an extraction method and application thereof, and belongs to the technical field of exosome-like nano-vesicle extraction. The extraction method of the aloe exosome-like nano-vesicles is designed and optimized, the yield of the aloe exosome-like nano-vesicles is obviously improved, and the extracted aloe exosome-like nano-vesicles can obviously delay skin photoaging and have an obvious improvement effect on skin fibroblast aging damage caused by ultraviolet radiation; a potential treatment means is provided for preventing or treating actinic keratosis caused by ultraviolet radiation and further possible squamous cell carcinoma.

The invention relates to a medical simulation experiment method and device for acting apoptosis vesicles on primary cartilage cells based on cell line culture, and the method mainly comprises the following steps: S1, co-culture system construction, S2, multi-dimensional observation and analysis, and S3, operation risk assessment. In the step S1, primary cartilage cells, primary immune cells and cell line culture apoptosis vesicles are co-cultured; s2, carrying out multi-dimensional observation and analysis on the influence of apoptosis vesicles on cells; and S3, mainly constructing a multi-dimensional risk scoring system, and carrying out risk grade division by applying a biological network model. The invention aims at simulating and evaluating the influence of cell line culture apoptosis vesicles on primary cartilage cells in vitro and the interaction between the cell line culture apoptosis vesicles and primary immune cells, so that an experimental basis is provided for subsequent clinical application.

The present disclosure provides a vaccine composition wherein the antigen components comprise a factor H binding protein (fHbp), a recombinant outer membrane vesicle (OMV) carrying porin A (PorA protein), and optionally a heparin binding protein (NHBA protein). The antigen components in the vaccine composition disclosed by the invention have a good synergistic effect, so that the vaccine composition has extremely high vaccine forming potential, is used for preventing epidemic of group B meningococcus, and plays a role in protecting infection of group B meningococcus.

The invention relates to a baicalin-cyclodextrin-vesicle compound as well as a preparation method and application thereof. Specifically, the invention discloses a baicalin-cyclodextrin-vesicle compound, the baicalin-cyclodextrin-vesicle compound comprises (i) an active component, and the active component comprises baicalin; (ii) a cyclodextrin; and (iii) a vesicle material, the vesicle material comprising vesicles of microbial origin; in addition, the cyclodextrin (ii) comprises the active ingredient (i) to form nanoparticles; and the (iii) vesicle material wraps the nanoparticles to form a baicalin-cyclodextrin-vesicle compound. The baicalin-cyclodextrin-vesicle compound has the performance of high transdermal efficiency, high bioavailability and high drug loading capacity, has the functions of aging resistance, oxidation resistance, whitening, inflammation resistance and the like, and can be applied to cosmetics.

The invention discloses a preparation method of rhizoma polygonati exosome microcapsules, and belongs to the technical field of biology, the rhizoma polygonati exosome microcapsules are prepared by four steps of crude extraction of rhizoma polygonati exosome, purification of the rhizoma polygonati exosome, preparation of rhizoma polygonati exosome freeze-dried powder and preparation of the rhizoma polygonati exosome microcapsules. The rhizoma polygonati exosome is wrapped in the tiny vesicles formed by the high polymer material, so that the external environment is effectively isolated, a protective barrier is provided for the exosome, the exosome is prevented from being degraded or inactivated in the external environment, and the stability of the exosome is improved; meanwhile, the polygonatum kingianum exosome can be prevented from being subjected to in-vivo enzymolysis and clearing through micro-capsule wrapping, the in-vivo residence time of the polygonatum kingianum exosome is prolonged, and the bioavailability is improved; the preparation process is simple, the operability is high, and the prepared exosome microcapsule has good application in the aspect of inhibiting gastric cancer cells.

The invention relates to Chinese pulsatilla root and stem exosome-like vesicles as well as a preparation method and application thereof. The preparation method comprises the following steps: juicing Chinese pulsatilla roots, filtering, and collecting filtrate; and centrifuging the filtrate, collecting the precipitate, re-suspending, and filtering to obtain a solution containing the exosome-like vesicles of the Chinese pulsatilla roots. Compared with the prior art, the Chinese pulsatilla root and stem exosome-like vesicles extracted by the method can inhibit malignant progression, including proliferation and migration, of lung cancer, and cell uptake and animal living imaging prove that the Chinese pulsatilla root and stem exosome-like vesicles can be taken by cells and can be enriched in tumor tissues at the same time.

The invention discloses a honeysuckle leaf-derived exosome-like nano-vesicle functional miRNA (micro Ribonucleic Acid) and application thereof, the honeysuckle leaf-derived exosome-like nano-vesicle functional miRNA is miR-novel1 or ath-miR396b-5p, the nucleotide sequence of the miR-novel1 is as shown in SEQ ID NO.1, and the nucleotide sequence of the ath-miR396b-5p is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the honeysuckle leaf-derived exosome-like nano-vesicle functional miRNA and application of the honeysuckle leaf-derived exosome-like nano-vesicle functional miRNA. The invention also specifically discloses an application of the functional miRNA in preparation of anti-inflammatory drugs. The functional miRNA in the honeysuckle leaf exosome-like nano-particles obviously inhibits transcription of pro-inflammatory cytokine genes and expression of protein for synthesizing pro-inflammatory mediator enzymes (iNOS and COX-2) by inhibiting an STAT3 signal channel under the condition of no cytotoxic effect, so that the anti-inflammatory effect is achieved, and the honeysuckle leaf exosome-like nano-particles can be used as a new choice for preparing anti-inflammatory drugs and other anti-inflammatory products.

The invention relates to the technical field of plant exosome-like extracts, in particular to a liquorice exosome-like extract as well as extraction and application thereof. The method comprises the following steps: firstly, removing impurities such as cells, cell debris, organelles, large vesicles and the like in homogenate by adopting a combination mode of low-speed centrifugation and medium-speed centrifugation, and further purifying the homogenate by adopting high-speed centrifugation after the low-speed centrifugation and the medium-speed centrifugation are completed, so as to obtain the exosome-like extract. And the setting of the increasing rotation speed is beneficial to finer separation of exosome-like particles with different sizes and densities, so that the purity and quality of the extract are improved. According to the method, all centrifugal operations are carried out in a low-temperature environment of 4 DEG C; the low-temperature condition is beneficial to maintaining the biological activity and stability of the sample, and the problems of protein denaturation, enzyme inactivation and the like caused by temperature rise are reduced, so that the quality and performance of the exosome-like extract are ensured.

The invention relates to an aloe extract nano-vesicle as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The aloe extract nano-vesicle provided by the invention is a nano-micelle formed by an aloe extract and tocopheryl acetate, the particle size of the aloe extract nano-vesicle is 280 to 500 nm, the Zeta potential of the aloe extract nano-vesicle is-10.12 to-11.34 mV, and the polydispersity coefficient of the aloe extract nano-vesicle is 0.239 to 0.434. The aloe extract nano-vesicle disclosed by the invention has a nano-scale size, and can easily penetrate through a skin barrier and enter skin cells, so that the moisturizing effect of the aloe extract nano-vesicle is achieved. The permeability of the moisturizing composition is far better than that of the traditional moisturizing composition, so that the required moisture can be more effectively provided for the skin. The aloe extract nano-vesicle can carry other active ingredients such as moisturizing factors to deeply permeate into skin, comprehensive care is provided for the skin, the texture and appearance of the skin are improved, the preparation process is simple, and the aloe extract nano-vesicle is suitable for technological production.

The invention belongs to the technical field of bionic nano-drugs, and particularly relates to a bionic vesicle preparation as well as a preparation method and application thereof. The bionic vesicle preparation disclosed by the invention is prepared from the following raw materials: mesenchymal stem cell outer vesicles, nintedanib and DSPE-PEG-HA (hyaluronic acid modified pegylated phospholipid); the nintedanib is loaded in the mesenchymal stem cell outer vesicle, and the DSPE-PEG-HA is coupled to the surface of the mesenchymal stem cell outer vesicle. The bionic vesicle preparation disclosed by the invention can be used for directly delivering a drug to a pulmonary fibrosis injury part through inhalation type drug delivery, and acting on a focus part at a higher local concentration, meanwhile, the exposure of the drug in whole-body circulation is remarkably reduced, the occurrence of side effects is reduced, and the specific targeting intervention and regulation on fibroblasts are realized.

The invention relates to the technical field of medicines, in particular to a curcumin-loaded engineered cell membrane nano-vesicle NVs-CUR and a preparation method thereof, and the preparation method comprises the following steps: S1, extruding a macrophage suspension through an extruder to obtain an NVs resuspension; and S2, adding the CUR mixed solution into the NVs resuspension solution, and carrying out ultrasonic treatment to obtain the nano vesicle NVs-CUR. As a novel drug delivery system, the NVs-CUR shows excellent performance in the aspects of physical stability, drug release and stability, cellular uptake ability, cytotoxicity and the like. The characteristics provide powerful support for application in treatment of diseases such as nerve injury and the like, and indicate the wide prospect in the field of biomedicine.

The invention discloses a therapeutic nano-vesicle preparation rich in mitochondrial functional protein, a preparation method of the therapeutic nano-vesicle preparation and application of the therapeutic nano-vesicle preparation in treatment of radioactive skin injury. The preparation method comprises the following steps: culturing, amplifying and cleaning umbilical cord mesenchymal stem cells, dispersing the umbilical cord mesenchymal stem cells in a suspension, mechanically extruding the umbilical cord mesenchymal stem cells into a crude solution through a multistage aperture polycarbonate membrane, and centrifuging, concentrating and purifying the crude solution to obtain the preparation. The preparation has the remarkable advantages that local administration can be realized, inflammation can be quickly inhibited, oxidative stress is reduced, and a repair pathway is activated, so that the preparation is suitable for treating acute radioactive skin injury; the preparation method is easy to standardize and scale, high in stability and convenient for emergency storage and delivery, and avoids the defects of cell therapy; a plurality of targets such as immune inflammation, oxidative stress and the like can be synergistically regulated, the biological effect of hUMSCs is simulated and partially replaced, and the limitation caused by the cell survival rate and the microenvironment dependency is avoided; the key function of hUMSCs is covered in function, and the clinical feasibility, safety and emergency suitability of the application level are higher.

Disclosed are compositions containing exosomes loaded with arginase or arginase chimeras for treating hyperammonemia due to arginase deficiency, and methods for treating hyperammonemia by administering compositions containing exosomes loaded with arginase or arginase chimeras.

The invention discloses a bone joint repairing composition containing stem cell vesicles and application of the bone joint repairing composition, and relates to the technical field of bone joint repairing compositions.The bone joint repairing composition is prepared from, by weight, 90-110 parts of stem cell vesicles and 41-57 parts of thelenota ananas saponin Ananaside A. The stem cell vesicles and the thelenota ananas saponin Ananaside A are combined to form the bone joint repairing composition. The stem cell vesicles are extracellular vesicles derived from human amniotic mesenchymal stem cells. According to the bone joint repairing composition provided by the invention, the stem cell vesicles and the thelenota ananas saponin in a proper proportion are matched for use, so that the synergistic interaction between the stem cell vesicles and the thelenota ananas saponin is realized. Animal experiments show that the composition has a remarkable repairing effect on a rat model with bone and joint injuries, can effectively relieve bone and joint pain symptoms of rats, not only widens the application field of human amniotic mesenchymal stem cell-derived extracellular vesicles, but also provides a new clinical treatment means for bone and joint repairing.

The invention discloses ZIF-8 loaded M2 macrophage outer vesicle hydrogel as well as a preparation method and application thereof. The preparation method comprises the following steps: inducing M0 macrophages through IL-4 to obtain M2 type macrophages; collecting cell supernate, and separating by adopting an ultracentrifugation method to obtain the M2 type macrophage extracellular vesicle EV; purifying the obtained M2-type macrophage extracellular vesicle EV, mixing the purified M2-type macrophage extracellular vesicle EV with ZIF-8 nanoparticles, and carrying out electroporation treatment to obtain a ZIF-loaded exosome vesicle ZIF (at) EV; performing ultrasonic stirring and mixing on a sodium alginate solution and calcium carbonate, adding the obtained ZIF-coated EV and D-(+)-gluconic acid delta-lactone, and fully stirring and mixing to obtain the product. The ZIF-EV hydrogel disclosed by the invention is effective in wound healing, and the ZIF-EV hydrogel can continuously release the ZIF-EV on the wound to promote wound healing.

The invention belongs to the technical field of regenerated medical dressings, particularly relates to a plant polysaccharide hydrogel medical dressing for promoting tissue damage repair and application thereof, and provides the following scheme that a medical and mechanical combined product comprises a regenerated medical dressing and a low-temperature sterile storage box for storing the regenerated medical dressing, the regenerated medical dressing comprises a hydrogel main body, open pores formed in the hydrogel main body, sustained-release vesicles arranged in the open pores, and stem cells and a cell culture medium which are filled in the sustained-release vesicles. The stem cell hydrogel can rapidly break blood, rapidly stop bleeding by being attached to a wound surface and promote wound closing, stem cells are inoculated to the upper surface of the hydrogel formed by dendrobium polysaccharides, aloe, lily, coptis chinensis and honeysuckle, safe application of the stem cells is achieved, active factors secreted by the stem cells are slowly released to the skin wound surface, and the wound healing effect is achieved. Through contact stimulation between cells, various cytokines can be secreted better, and the skin repair efficiency is improved.

The invention provides a method for detecting an external vesicle marker through high-flux nano plasma exciting light immune color development, and belongs to the technical field of human extracellular vesicles. After a serum sample is subjected to centrifugal treatment, the serum sample and a CD81 capture antibody substrate are incubated, and the particle size distribution of the vesicles is monitored in real time; a zwitterionic polymer modified gold nanoparticle LAM detection probe and a polyethylene glycol modified silver nanoparticle LprG detection probe are prepared to be specifically combined with a vesicle surface antigen, a chromogenic enhancement solution is adopted to induce a plasma resonance signal, and full-hole scanning imaging is carried out; and constructing a double-layer game optimization model to cooperatively optimize the detection sensitivity and the signal stability, carrying out weighted summation on normalized signals of the particle size subgroups to obtain comprehensive detection signal intensity, comparing the comprehensive detection signal intensity with a threshold value, and outputting a final judgment result. The technical problem that quantitative accuracy is affected by signal intensity deviation caused by vesicle particle size difference in outer vesicle marker detection is solved.

The invention discloses a preparation method of polygonatum-sibiricum-sourced exosome-like nano-vesicles and application of the nano-vesicles in antitumor drugs, and relates to the technical field of biological medicines. The preparation method comprises the following steps: crushing fresh rhizoma polygonati slices, filtering and collecting to obtain a first filtrate; carrying out gradient centrifugation treatment on the first filtrate to obtain supernate; carrying out first membrane filtration treatment on the supernate to obtain second filtrate with the particle size of less than 450 nm; centrifuging the second filtrate to obtain a precipitate, and resuspending the precipitate with a PBS buffer solution to obtain a rhizoma polygonati exosome crude extract; and performing secondary membrane filtration treatment on the rhizoma polygonati exosome crude extract to obtain a third filtrate with the particle size of less than 220nm, thereby obtaining the rhizoma polygonati-derived exosome-like nano-vesicles. The polygonatum-sibiricum-sourced exosome-like nano-vesicle has remarkable inhibitory activity on tumors, provides a new strategy and means for tumor treatment, and has important scientific value and clinical application potential.

The invention discloses an engineered bionic nucleic acid nano-vesicle as well as a preparation method and application thereof, relates to the technical field of nano biomedicine, and aims at solving the problems that in-vivo targeting efficiency and immunogenicity of a traditional cationic lipid nano-carrier are limited due to deletion and cleavage obstacles of GSDMD expression in tumor cells. The technical key point of the invention is as follows: the engineered bionic nucleic acid nano-vesicle is provided and is prepared by wrapping a cationic lipid nucleic acid drug with an exosome derived from engineered macrophages; wherein the exosome from the engineered macrophage is the exosome from the macrophage with high expression of PD1, which is as shown in SEQ. ID. NO.1. The exosome from the engineered macrophage is the exosome from the macrophage with high expression of PD1; the lipid nucleic acid medicine is prepared by loading GSDMD-N mRNA (messenger Ribonucleic Acid) shown on the basis of SEQ.ID.NO.2 on a cationic liposome. The engineered bionic nucleic acid nano-vesicle is used for preparing an oral squamous cell carcinoma diagnostic kit and a therapeutic drug.

The invention provides a plug-and-play Mi3 protein nanocage fusion protein with a VNP6 tag and a preparation method and application of the plug-and-play Mi3 protein nanocage fusion protein, the fusion protein comprises the VNP6 tag, a SpyCatch003 component, a Mi3 protein nanocage and a functional protein sequence, and the VNP6 tag is located at the N end of the fusion protein. The VNP6 peptide sequence or the variant thereof is utilized to induce a vesicle or vesicle-like structure beneficial to protein folding and stabilization in escherichia coli, so that the correct folding rate and activity of recombinant protein are improved while the overall intracellular molecular crowding effect is relieved. The core idea of the invention lies in that the internal structure of cells is regulated at low temperature, and the internal microenvironment of an escherichia coli expression system is optimized, so that protein molecules under high expression load can obtain more reasonable spatial distribution, and the problems of misfolding and aggregation caused by molecular crowding are reduced.

The invention relates to the technical field of biological medicine, in particular to immunogen cell death enhanced nano-vesicle hydrogel and application thereof. The invention provides a 3D-printable multifunctional synergistic immunogen cell death enhanced nano-vesicle hydrogel, which is characterized in that SerMA hydrogel is used as a matrix, cell membrane nano-vesicles (N (at) ILO) loaded with a photothermal agent IR1061, a chemotherapeutic drug OXA and a tumor acidic microenvironment regulator Lon are encapsulated, initial tumor immunogen cell death is triggered through a photothermal effect, and the tumor immunogen cell death enhanced nano-vesicle hydrogel can be used for 3D printing. OXA further enhances the ICD effect, Lon regulates the acidic tumor microenvironment to improve the T cell activity, and postoperative multi-mode synergistic anti-tumor treatment is achieved; meanwhile, the hydrogel also has excellent photocuring 3D printing capability, can be used for individually constructing a filling structure matched with a postoperative defect area, has a wide application prospect, and provides a new strategy and theoretical basis for postoperative comprehensive treatment of choroidal melanoma.

The invention discloses a Gegen Qinlian decoction vesicle-like nanoparticle as well as an extraction method and application thereof, and belongs to the technical field of biological medicines. The method comprises the following steps: preparing a radix puerariae qinlian decoction stock solution; placing at 4-8 DEG C for 12-15 hours to obtain a gelatinous substance; carrying out low-speed centrifugation at 4-8 DEG C, and taking supernate, so as to obtain crude extract of the vesicle-like nanoparticles of the radix puerariae, radix scutellariae and rhizoma coptidis decoction; carrying out ultracentrifugation at 4-8 DEG C, discarding the supernatant, and carrying out PBS resuspension precipitation to obtain a crude product of the vesicle-like nano-particles of the Gegen Qinlian decoction; and centrifuging 16000g at the temperature of 4-8 DEG C for 10-15 minutes, taking supernate, and filtering by using a 0.22 mu m filter membrane, so as to obtain the vesicle-like nanoparticles of the Gegen Qinlian decoction. According to the Gegen Qinlian decoction vesicle-like nanoparticles as well as the extraction method and the application thereof, the bioavailability and the targeting property of effective components in the Gegen Qinlian decoction are remarkably improved through a nanocrystallization technology, the concentration of the medicine at the ulcerative colitis part is enhanced, and the treatment effect is effectively improved.

The invention provides a recombinant engineering bacterium for preparing recombinant human albumin based on a transport secretion way and application, and relates to the technical field of gene engineering. One or more of endoplasmic reticulum related genes SRP54 and ERJ5, vesicle transport related genes YOS1 and SLY1 and Golgi apparatus related genes GOS1 and YIP1 are integrated into a recombinant engineering bacterium genome through a gene recombination technology, so that efficient secretory expression of recombinant human albumin is realized. According to the method provided by the invention, the expression quantity of the recombinant human albumin is remarkably improved, and the method has a wide application prospect.

The invention belongs to the field of biological medicines, and relates to a drug-loaded nano-vesicle as well as a preparation method and application thereof. The drug-loaded nano-vesicle comprises a vesicle core and a drug-loaded nano-vesicle, wherein the vesicle core comprises siRNA (small interfering Ribonucleic Acid) capable of specifically targeting and silencing an NR1D1 gene; the vesicle membrane is formed by fusing an erythrocyte membrane, a macrophage membrane, cardiolipin, cholesterol and lecithin. The drug-loaded nano-vesicle can specifically target macrophages in a sepsis immunosuppression stage, has an intracellular response release function, recovers BMAL1 and IGF2BP2-ATP6V1B2 / ATP6V0c axis functions by inhibiting NR1D1 expression, reconstructs a macrophage phagocytosis function and lysosome-dependent bacterium removal capability, and can be used for preparing a drug-loaded nano-vesicle with a specific targeting function. The survival rate of sepsis immunosuppression model animals is obviously improved; and the bacterial load is reduced. Compared with a traditional electroporation method, the preparation method disclosed by the invention has the advantage that the encapsulation efficiency of siRNA is remarkably improved.