The invention discloses a pure natural makeup removal paste which is prepared from, by weight, 20-55% of vegetable fat, 10-35% of a surface active agent, 2-30% of solid wax, 0.1-5% of rosemary extract or tocopherol, 2-10% of sage essential oil, 3-6% of myrrh essential oil, 0.5-1% of carnation extract, 0.2-0.8% of citrus fruit extract and 0.2-0.6% of lotus extract, wherein the sum of the percentages of the compositions is 100%. The adopted natural vegetable fat contains no essence, synthetical pigment, preservative, mineral oil, mineral wax and other chemical raw materials harmful to human skin, and is safe, mild, free of stimulation and capable of nourishing skin while achieving makeup removal. In addition, by using the sage essential oil, the myrrh essential oil and the carnation extract in a cooperated mode, the good effects of repairing the skin surface lipid film, adjusting the skin balance value, promoting updating of damaged skin, thickening cuticle and replenishing and locking water are achieved. The invention further provides a preparation method of the makeup removal paste.