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Probiotics liposome and preparation method thereof

A technology of probiotics and liposomes, which is applied in the direction of liposome delivery, dairy products, milk preparations, etc., can solve problems such as no patent reports, and achieve improved stability and activity, stable system, increased survival rate and The effect of the number of colonization

Inactive Publication Date: 2019-08-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant patent reports at home and abroad about the preparation of probiotic liposomes

Method used

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  • Probiotics liposome and preparation method thereof
  • Probiotics liposome and preparation method thereof
  • Probiotics liposome and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Take 140 mg of soybean lecithin and 23 mg of cholesterol required for making liposomes with a ten-thousandth balance, and place them in a rotary steamer;

[0039] (2) Measure 5 mL of chloroform and add it to the rotary steamer, shake it to fully dissolve soybean lecithin and cholesterol, and form a clear and transparent mixed solution 1;

[0040] (3) Place the rotary evaporating bottle with mixed solution 1 at 45° C. on a 150rmp rotary evaporator for 30 min under reduced pressure to remove chloroform and form a uniform transparent film on the wall of the bottle. The steaming bottle was placed in a vacuum drying oven to dry for 12 hours to remove residual chloroform;

[0041] (4) prepare pH=7.0 with ultrapure water, the phosphate buffer solution that mass fraction is 0.40%;

[0042] (5) Take 10mL of phosphate buffer solution and 17.5mg of probiotic freeze-dried powder respectively, put them in a centrifuge tube, mix well to obtain mixed solution 2, and preheat to 40...

Embodiment 2

[0047] (1) Weigh 150 mg of soybean lecithin and 25 mg of cholesterol required for making liposomes with a ten-thousandth balance, and place them in a rotary steamer;

[0048] (2) Measure 8 mL of chloroform and add it to the rotary steamer, and shake to fully dissolve the soybean lecithin and cholesterol to form a clear and transparent mixed solution 1;

[0049] (3) Place the rotary evaporating bottle with the above mixed solution 1 at 45° C. on a 100rmp rotary evaporator for 40 min under reduced pressure to remove chloroform and form a uniform transparent film on the bottle wall, and then The rotary evaporator was placed in a vacuum drying oven for 12 hours to remove residual chloroform;

[0050] (4) prepare pH=7.2 with ultrapure water, the phosphate buffer solution that mass fraction is 0.42%;

[0051] (5) Take 10mL of phosphate buffer solution and 15mg of probiotic freeze-dried powder and place them in a centrifuge tube, mix well to obtain a mixed solution 2, and preheat to...

Embodiment 3

[0056] (1) Weigh 160 mg of soybean lecithin and 20 mg of cholesterol for making liposomes with a ten-thousandth balance, and place them in a rotary steamer;

[0057] (2) Take another 10 mL of chloroform and add it to the rotary steamer, shake it to fully dissolve the soybean lecithin and cholesterol, and form a clear and transparent mixed solution 1;

[0058] (3) Place the rotary evaporating bottle with the above mixed solution 1 at 50° C. on a 50rmp rotary evaporator for 60 min under reduced pressure, remove chloroform, and form a uniform transparent film on the wall of the bottle, and then The rotary evaporator was placed in a vacuum drying oven for 12 hours to remove residual chloroform;

[0059] (4) prepare pH=7.5 with ultrapure water, the phosphate buffer solution that mass fraction is 0.45%;

[0060] (5) Take 10mL of phosphate buffer solution and 14mg of probiotic freeze-dried powder respectively and place them in a centrifuge tube, mix thoroughly to obtain a mixed solu...

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Abstract

The invention discloses a probiotics liposome and a preparation method thereof. The particle size of the probiotics liposome is (143.87+ / -10.58)-(161.53+ / -11.62)nm, and the encapsulation rate of probiotics in the probiotics liposome is 40-57.14%. The method includes steps: (1) preparing a lipid film; (2) preparing phosphate buffer solution; (3) dissolving probiotics into the phosphate buffer solution to form mixed solution 2, and preheating; (4) forming liposome suspension; (5) subjecting the liposome suspension to ultrasonic treatment, filtration and hydration to obtain the probiotics liposome. The probiotics liposome is prepared aiming at the problem of proneness to inactivation of probiotics in a digestion process of the human body, stability and activity of encapsulated probiotics in the human body are improved, the survival rate and setting quantity of probiotics in the intestinal tract are increased, and accordingly probiotic effects of probiotics can be truly achieved.

Description

technical field [0001] The invention belongs to the field of food processing, and relates to a probiotic liposome and a preparation method thereof. Background technique [0002] Probiotics refer to living microorganisms in the human intestinal environment that are beneficial to the health of the body. Common probiotics include lactic acid bacteria such as Bifidobacterium, Lactobacillus, Streptococcus, Enterococcus, and some non-lactic acid bacteria such as Bacillus, yeast, and Propionibacterium. Studies have shown that probiotics are closely related to the health of the host. It can promote the digestion and absorption of nutrients, improve the body's immunity, maintain the balance of intestinal flora, improve the body's antioxidant capacity, inhibit intestinal inflammation, reduce serum cholesterol and protect the intestinal tract. mucous membrane barrier and many other functions. In recent years, there are more and more functional probiotic products on the market, includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/12A23C9/13A61K35/741A61K9/127A61K47/24A61K47/28A61P37/04A61P1/00A61P39/06A61P29/00A61P3/06
CPCA23C9/1203A23C9/1315A23C9/13A61K35/741A61K9/127A61K47/24A61K47/28A61P37/04A61P1/00A61P39/06A61P29/00A61P3/06
Inventor 黄惠华行云逸
Owner SOUTH CHINA UNIV OF TECH
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