The application provides a high-efficiency expansion culture method of
umbilical cord blood gamma
delta T cells and application thereof, and belongs to the technical field of biological
medicine. Through a specific two-stage strategy of "initial induction culture + expansion culture", the application is simple in operation, stable in
system, and high in success rate, effectively overcomes the defects of limited
autologous blood source, unstable expansion and low
cell activity in the prior art, and lays a foundation for large-scale preparation and allogeneic application of gamma
delta T cells. The
umbilical cord blood gamma
delta T cells obtained by the method of the application are excellent in quantity, purity, activity and anti-tumor function, and have stronger tumor
cell killing ability, and are especially suitable for
adoptive immunotherapy. The application breaks through the technical
bottleneck of the traditional culture
system, provides a high-efficiency, stable and clinically
convertible gamma delta T cell expansion scheme, and has important application prospect and market value.