20 results about "Histochemical staining" patented technology

The application relates to the field of immunohistochemical staining, in particular to a DAB buffer and a DAB staining solution kit for DAB color development. The application discloses a DAB buffer for DAB color development, and the main components are hydrogen peroxide, nonylphenol polyoxyethylene ether, magnesium sulfate and trisodium citrate. The metal ions in the DAB buffer can enhance the electron transfer speed between DAB and hydrogen peroxide, and improve the color development sensitivity. The nonylphenol polyoxyethylene ether can stabilize the hydrogen peroxide, so that the hydrogen peroxide can be stored for a longer time and the cost is saved. The application further discloses a DAB staining solution kit, which comprises a blocking agent, a reaction enhancer, an enzyme-labeled polymer IgG, a DAB concentrated solution, a DAB buffer and hematoxylin, and the above-mentioned DAB buffer for DAB color development. The DAB staining solution kit of the application can improve the detection sensitivity of immunohistochemical staining, and can also improve the storage stability of the DAB color development solution and reduce the cost.

The application provides an immunohistochemical staining image analysis method, including: cutting a plurality of cell nuclei of an immunohistochemical staining image according to a machine learning model; removing pixels belonging to the cell nuclei in the immunohistochemical staining image and removing pixels meeting a color range to obtain a plurality of cytoplasm pixels; distributing the cytoplasm pixels to the cell nuclei according to the positions of the cytoplasm pixels to form a plurality of cells; and calculating a pixel staining score of each pixel in the cells, thereby calculating a cell staining score of the cells. Thus, the staining score of the cells can be objectively and automatically calculated.

The present invention relates to: a composition and kit for immunohistochemistry (IHC) staining, comprising an anti-PAUF monoclonal antibody that specifically binds to a PAUF protein; and an immunohistochemical staining method using same. The present invention exhibits significantly improved staining sensitivity and clarity over conventional PAUF staining methods, thereby providing the advantage of obtaining more accurate and reliable results regarding the expression of the PAUF protein. In addition, the present invention enables accurate staining of a large amount of clinical tissues in a short time by using specialized staining equipment together, thereby providing the advantage of enhancing the reliability of staining results. Accordingly, it is possible to specifically, rapidly, and accurately identify only PAUF within cancer tissues of cancer patients, thereby enabling rapid and efficient cancer diagnosis, and by using this, early cancer diagnosis is possible, thereby enabling early treatment for cancer patients in need of targeted therapy.

A method for training a machine learning model and a computing system performing same, wherein the machine learning model is trained to analyze biological tissue slide images stained by a immunohistochemical staining method for staining tissues expressing specific biomarkers and thus can be used to further elaborately analyze expression levels of biomarkers, etc., whereby a determination can be made on pathological specimen images by analyzing expression levels of biomarkers, etc. A method and system for training a machine learning model using training data, corresponding to immunohistochemically stained images, generated from multiple feature vectors calculated based on various staining intensity criteria.

A method for preventing the retinal pigment from being removed from the network, comprising the steps of pre-fixing liquid fixation, solidifying agent wrapping shaping, fixing liquid fixation, embedding agent embedding, melanin decomposition liquid decomposing melanin, pre-decoloring liquid pre-decoloring, decoloring liquid decoloring and rinsing liquid elution. A method for removing melanin is provided to effectively avoid the retinal RPE layer cell from being removed from the network, protein denaturation and nucleic acid degradation. The method mainly adopts the following steps: pre-fixing liquid to maintain the original state of tissue cells, solidifying agent to maintain the integrity of tissue structure, melanin decomposition liquid composed of various extracts to decompose melanin particles, pre-decoloring liquid and decoloring liquid to separate melanin, and rinsing liquid to elute the decomposed melanin on the surface of the tissue, etc. Under the premise of ensuring the integrity of the retinal tissue structure, not damaging the surface protein, nucleic acid and cell structure of the tissue section, effectively decomposing the melanin particles in the tissue section, maintaining the biological activity of various organelles on the section surface and meeting the needs of subsequent various histochemical staining.

The present application relates to cell biology, and more specifically, to a multimodal biophysical characterization apparatus, a multimodal biophysical characterization system, a multimodal biophysical characterization method, and its use. The present application can achieve multimodal, high-resolution, high-throughput, high-sensitivity, low-cost, low-damage, and configurable physical characterization of single cells or multicellular aggregates, and can subject the sample to be tested to stimuli of different types and intensities (such as mechanical stimulation, electrical stimulation, light stimulation, etc.) to better simulate the in vivo microenvironment, as well as perform operations on samples at specific positions or regions (such as marking, fixing, ablating, cutting, extracting, sorting, etc.), and combine with other characterization methods (such as protein staining, histochemical staining, single-cell sequencing, etc.) for comparative analysis. The multimodal biophysical characterization apparatus provided by the present application is suitable for fields such as synthetic biology, diagnostics, drug discovery, early tumor screening, cell therapy, and precision medicine.

To provide a quality control slide which enables estimation of the presence or absence of abnormality in an automatic immunostaining device and causes thereof if abnormality is present.SOLUTION: A quality control slide is provided, having a spot (antigen(-)) containing no antigen, a spot (fixation(-) / antigen(+)) not histologically fixed and containing an antigen, and a spot (fixation(+) / antigen (+)) histologically fixed and containing the antigen.SELECTED DRAWING: Figure 1

A supramolecular histochemistry system for staining includes a chaotropic ion and a chaotropic ion complexing agent that acts as a molecular host and accepts the chaotropic ion as a molecular guest. The chaotropic ion facilitates the diffusion of a probe, such as an antibody, into and within a tissue sample, binding the probe to the molecular host or diluting the probe, thereby promoting the association of the antibody with a target antigen and generating an immunostaining or histochemistry signal. Alternatively, a small fluorescent probe serves as the molecular guest and is complexed by the supramolecular host to facilitate deep penetration. A method for performing histology employs the supramolecular system with deep, uniform histochemistry. Such a method is relatively rapid, scalable, automatable, and cost-effective.

The utility model discloses an anti-leakage immunohistochemical staining machine heating structure, which comprises a heating block and a flow guide block connected with the heating block, the heating block is arranged below a slide, a top slot and a bottom slot are arranged at the joint of the heating block and the flow guide block, and the flow guide block is arranged in the top slot. An opening of the top opening groove faces the direction of the slide, an opening of the bottom opening groove deviates from the direction of the slide, and a liquid drainage hole is formed in the flow guide block and located in the outer side of the top opening groove and the outer side of the bottom opening groove. The bottom groove and the flow guide plate are additionally arranged between the heating block and the flow guide block, liquid such as reagents overflowing from the slide is guided and collected, the liquid such as the reagents is prevented from permeating into the heating film to damage the heating film, the problem of corrosion or stripping of the heating film is avoided, reliable and efficient heat transfer between the heating film and the supporting body is guaranteed, and the service life of the heating film is prolonged. The equipment failure rate is reduced, and the service life of the immunohistochemical staining equipment is prolonged.

The invention discloses a gastric cancer tumor immune microenvironment typing method and system and application in prognosis prediction. The method comprises the following steps: carrying out multiple immunohistochemical staining on a patient tissue sample and obtaining a full-view image, segmenting a tumor region and a matrix region in the image, identifying CD3 + T cells and counting, and calculating the CD3 + T cell density of the two regions and the CD3 + T cell ratio of the matrix region. Typing according to a threshold value type 3: the tumor region with CD3 + T cell density greater than or equal to 1000 cells / mm < 2 > is an immune inflammation type; the tumor region CD3 + T cell density is lt; 1000 cells / mm < 2 > and the ratio of CD3 + T cells in a matrix region is 1t; 0.18 is an immune desert type; the tumor region CD3 + T cell density is lt; and if the ratio of CD3 + T cells in a matrix region is greater than or equal to 0.18, the cells are in an immunoexclusion type. The method can provide risk stratification information (data prompt) related to OS / PFS in vitro.

The invention belongs to the field of wheat immature embryo genetic transformation, and discloses a method for constructing and optimizing a wheat immature embryo vacuum infiltration transient transformation system, which comprises the following steps: by taking a wheat variety Fielder immature embryo pollinated for 14 days as a material, optimizing vacuum infiltration pressure and treatment times, and utilizing GUS histochemical staining, conductivity determination and qRT-PCR gene expression analysis, and establishing a wheat immature embryo vacuum infiltration transient transformation system. And evaluating the relationship between the transformation efficiency and the cell damage degree. According to the method disclosed by the invention, a transformation system combining 0.025 MPa with four times of vacuum infiltration treatment is established by a synergistic mechanism of low pressure of 0.025 MPa and four times of infiltration, so that the GUS positive rate of the wheat Fielder immature embryo is increased to 62.8%, and an effective technical support is provided for wheat gene function analysis, CRISPR editing and molecular design breeding.

Diagnosis based on antigen-specific detection can be performed by other types of tissue staining with lower cost and wider availability.SOLUTION: The present disclosure relates to techniques for obtaining composite immunohistochemistry (IHC) images from histochemical stain images. Among other things, aspects of the present disclosure are directed to accessing an input image depicting a tissue section that has been stained with at least one histochemical stain, generating a synthetic image by processing the input image using a trained generator network, and outputting the synthetic image. The composite image depicts a tissue section that has been stained with at least one IHC stain directed to a first antigen, and the techniques may also include receiving an input based on an expression level of the first antigen from the composite image and / or generating a value based on the expression level of the first antigen from the composite image.SELECTED DRAWING: Figure 15A

The present disclosure relates to techniques for obtaining a synthetic immunohistochemistry (IHC) image from a histochemically stained image. Particularly, aspects of the present disclosure are directed to accessing an input image that depicts a tissue section that has been stained with at least one histochemical stain; generating a synthetic image by processing the input image using a trained generator network; and outputting the synthetic image. The synthetic image depicts a tissue section that has been stained with at least one IHC stain that targets a first antigen, and techniques may also include receiving an input that is based on a level of expression of a first antigen from the synthetic image and / or generating, from the synthetic image, a value that is based on a level of expression of the first antigen.

This invention pertains to the clinical application of traditional Chinese medicine, primarily focusing on the application of a Chinese herbal extract in the prevention and treatment of endometriosis. The invention also relates to a method for preparing this extract. The preparation method involves: taking 250 g of raw Curcuma zedoaria slices and heating them under reflux with 15 times the volume of 90% ethanol, extracting twice, 1.5 h each time; then extracting the residue with 10 times the volume of distilled water for 1.5 h; combining the ethanol and water extracts, filtering, and concentrating under reduced pressure at 55°C to a crude drug content of 1.93 g / mL. In vivo experiments have demonstrated that the extract significantly reduces the volume of ectopic endometrium. HE staining in nude mice in the endometrial lesion group revealed extensive inflammatory infiltration and angiogenesis in the ectopic endometrial lesions. Administration of the extract significantly reduced inflammatory infiltration and angiogenesis. Immunohistochemical staining of nude mice in the EMs group revealed high positive rates of PCNA and MVD cells. After administration of the herbal extract of this invention, the positive rates of PCNA and MVD cells were significantly reduced, demonstrating a significant anti-endometriosis effect.

The invention relates to the technical field of medical research, in particular to a method for evaluating the invasive growth mode of hepatocellular carcinoma based on multiple pathological staining technologies, which comprises the following steps: taking liver tissue, pretreating a specimen, preparing a slice, and obtaining a specimen slice; dyeing the specimen sections by using hematoxylin-eosin dyeing, and analyzing the dyeing condition; the method comprises the following steps: respectively carrying out masson staining and specific immunohistochemical staining on a key junction region of a specimen section on the basis of a staining condition, and evaluating an invasive growth mode of hepatocellular carcinoma, so that the invasive growth mode of hepatocellular carcinoma is evaluated by carrying out multi-dimensional observation and analysis on the junction region of hepatocellular carcinoma tissue and multiple adjacent structures; three pathological technologies of basic morphology, fibrosis evaluation and specific cell localization immunohistochemical staining are combined, mutual verification is carried out, the limitation of a single technology is made up, and a new pathological basis is provided for accurately knowing the growth characteristics and invasion potential of hepatocellular carcinoma and formulating an individualized diagnosis and treatment strategy.

The present invention describes an immunohistochemical staining device, which comprises dispensers for supplying reagents precisely so as to correctly administer same, with a control module also being installed in the device to automate the staining operation. Another feature of the immunohistochemical staining device is that it includes a touch screen for carrying out adjustments and for operation. The device also has reagent containers arranged radially for correct distribution on staining plates, in addition to including an elevator with rods for the vertical positioning of slide plates.

This application relates to the field of cell biology, and more specifically to a multimodal biophysical characterization apparatus, a multimodal biophysical characterization system, a multimodal biophysical characterization method, and its applications. This application enables multimodal, high-resolution, high-throughput, high-sensitivity, low-cost, low-damage, and configurable physical characterization of single cells or multicellular aggregates, and allows for the application of different types and intensities of stimuli (e.g., mechanical, electrical, or optical stimulation) to the sample under measurement to better mimic the in vivo microenvironment, and allows for manipulation (e.g., labeling, fixation, ablation, cutting, extraction, or sorting) of samples at specific locations or regions, and can be combined with other characterization methods (e.g., protein staining, histochemical staining, or single-cell sequencing) for comparative analysis. The multimodal biophysical characterization apparatus according to this application is suitable for fields such as synthetic biology, diagnostics, drug discovery, early tumor screening, cell therapy, and precision medicine.

The present application relates to a method and application for changing the fiber cell wall thickness and lignin content of poplar wood tissue, and belongs to the technical field of plant molecular breeding; the steps are as follows: S1: cloning of the genomic sequence and coding sequence of PtoMYB10; S2: screening of PtoMYB10 target site knockout and vector construction; S3: creation of PtoMYB10 knockout mutants. The method and application for changing the fiber cell wall thickness and lignin content of poplar wood tissue of the present application, by knocking out the PtoMYB10 gene of poplar, the mutant plants show higher growth vigor, compared with the wild type, the plant height and ground diameter are not inhibited by growth; the mutant plants show significantly reduced xylem fiber cell wall thickness; the mutant plants do not show significant changes in vessel cell wall thickness, the vessels maintain normal morphology without collapse; histological staining shows that the lignin content in the xylem of the mutant plants is reduced; the present application provides a genetic resource for molecular genetic improvement of forest trees oriented towards wood biomass development and utilization.

The invention relates to a kit for predicting colorectal cancer liver metastasis risk and prognosis, and belongs to the technical field of biomedicine. The kit comprises: (a) an antibody for specifically recognizing phosphoglycerate kinase 1 (PGK1) protein; and (b) a scoring reference for semi-quantitative scoring of PGK1 protein immunohistochemical staining results. The invention provides a colorectal cancer liver metastasis risk and prognosis evaluation solution which is accurate in prediction, easy to popularize, new in mechanism and wide in application, effectively overcomes the defects of insufficient sensitivity, low specificity and lack of molecular mechanism basis of the existing clinical prediction means, has outstanding substantive features and remarkable progress, and has a wide application prospect. The social benefit and the economic benefit are obvious.

The invention relates to the technical field of biomedical detection, and particularly discloses a multiple histochemical staining method based on controllable staining-decolorizing circulation. The method is realized through the steps of water bath-assisted antigen repair, composite pretreatment, alternate labeling dyeing of nanogold and quantum dots, electrochemical-assisted controllable decoloration, dynamic monitoring circulation, optical transparency treatment, multi-modal imaging reconstruction and the like. The key composition comprises a composite decolorizing agent containing a specific electron shuttling medium and a series of coloring agents containing nanogold and quantum dots. The preparation method mainly comprises the following steps: performing incubation reaction on each component and a tissue sample according to a specific sequence, completing controllable decolorization circulation under the assistance of an electric field, and regulating and controlling the process in real time through an optical monitoring system. According to the method disclosed by the invention, the sequential marking and accurate recognition of multiple target molecules on the same tissue sample can be realized; in addition, the preparation method has the advantages of being high in process controllability, good in dyeing cycle stability and high in operation repeatability.