The invention relates to a rice cultivating method capable of improving resistant starch content, which includes the steps: taking a mature embryo of a rice variety with amylase content larger than 25% as an explant, inducing callus, subculturing to a multiplication period, preculturing under the condition of dark, conducting agrobacterium co-culture transformation, transferring to a screening medium to conduct three turns of screening, taking resistant callus to be induced to differentiate seedlings, conducting histochemical staining on marked genes of a resistant plant, conducting molecular detecting on screened genes of the resistant plant, identifying differentiation little seedlings containing a target gene starch synzyme ssIII-2 insertion sequence, conducting detecting of the resistant starch content of hot rice on selected transgenosis plants, selecting transgenosis plants with the resistant starch content larger than 10%, continuously planting, evaluating expression stability of the resistant starch content, and propagating excellent strains with the resistant starch content larger than 10% stably. The rice with improved resistant starch content has significant meaning on conducting dietotherapy for diabetes patients.