Drought-induced rice flower specific promoter and use thereof

A drought induction and promoter technology, applied in plant promoters and their application fields, can solve the problems of other gene expression and effect, consumption of plant energy, etc.

Active Publication Date: 2009-04-22
BEIJING KAITUO DNA BIOTECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In some cases, the introduced foreign gene needs to be specifically expressed in a certain tissue within a certain period of time. Constitutive expression not only consumes a lot of energy of the plant itself, but also may affect the expression and function of other genes at the same time

Method used

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  • Drought-induced rice flower specific promoter and use thereof
  • Drought-induced rice flower specific promoter and use thereof
  • Drought-induced rice flower specific promoter and use thereof

Examples

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Embodiment Construction

[0023] The methods used in the following examples are conventional methods unless otherwise specified. The primers used were synthesized by Shanghai Handsome Biotechnology Co., Ltd., and the sequencing was completed by Shanghai Boya Biotechnology Co., Ltd. Enzymes and T4 DNA polymerase used for blunt end were purchased from Zibao Bioengineering Co., Ltd., and the ligation kit was from Invitrogen Company. The methods were all carried out according to the methods provided in the kit. The vector pCAMBIA1303 used in the experiment comes from CAMBIA. See Table 1 and image 3 .

[0024] 1. Chip hybridization microarray data selection promoter

[0025] 1.1 Plant material and treatment

[0026] It is used for gene expression microarrays in rice. For its production method and sources, see "Conservation and divergence of light-regulated genome expression patterns during seedling development in rice and Arabidopsis" Plant Cell 17, 3239-3256 and "A microarray analysis of the rice trans...

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Abstract

The invention discloses a drought induced rice flower-specific promoter and application thereof. The promoter has a nucleotide sequence shown in SEQ ID No.1, or a nucleotide sequence which can be hybridized with the sequence under highly rigorous conditions and has same functions. The promoter has flower tissue specificity and shows certain inductivity under drought induction conditions. By constructing a plant expression vector containing the promoter, the promoter is transformed into transgenic rice, the GUS histochemical staining and the RT-PCR detection are performed to a plurality of different tissues of a transgenic material such as ordinary leaves, sword-like leaves, flowers and so on under the drought induction conditions and the result proves the expression of a driving gene of the promoter in a rice flower tissue under the drought induction. The cloning of the promoter has greater practical significance on the research of male sterile strains of rice, and can be used for creating sterile lines of the rice and other plants and maintaining line-double-lines.

Description

technical field [0001] The invention relates to a plant promoter and its application, in particular to the cloning and functional analysis of a rice promoter exhibiting tissue specificity and drought inducibility. Background technique [0002] The formation of floral organs during the development of higher plants is a very complex process, which includes a series of organ differentiation and strictly controlled cellular and biochemical changes, accompanied by the coordinated expression of a large number of genes. Traditionally, the promoters of plant genes are divided into constitutive promoters (the gene expression is not limited by time and space and expressed in plants by the induction of certain substances) and inducible promoters (the The expression of the gene is induced by a certain substance, without the presence of the inducer, the gene expression level is very low or even absent) and a tissue-specific promoter (the expression of the gene is distributed in a certain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H1/00A01H5/00
CPCC12N15/8289C07K14/415C12N15/823C12N15/8273
Inventor 刘敏翟晨光邓兴旺夏勉
Owner BEIJING KAITUO DNA BIOTECH RES CENT
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