67 results about "Tissue specificity" patented technology

Provided is the use of overexpression of the auxin transporter PtoPIN1a gene in increasing the biomass of Populus tomentosa. The nucleotide sequence of the auxin transporter PtoPIN1a gene is as shown in SEQ ID No. 1. By means of using a genetic engineering method, wild-type Populus tomentosa is transformed by means of Agrobacterium tumefaciens infection to obtain a tissue-specific PtoPIN1a overexpressing transgenic plant, and subsequently, a plant with a relatively high expression level is selected, by means of positive identification, for phenotype observation. Results show that compared with the wild type in the same period, the PtoPIN1a transgenic plant has an increased plant height, a thicker stem and a significantly increased aboveground biomass. By means of the provided technical cultivation method, a high-biomass high-quality poplar variety is cultivated. The method is of great significance for future molecular breeding to cultivate excellent poplar varieties.

The invention relates to the technical field of plant genetic engineering, and particularly provides a rice endosperm tissue specific promoter pPROLM26 and application thereof. According to the invention, a tissue-specific promoter with a nucleotide sequence as shown in SEQ ID NO.1 is separated from a rice alcohol-soluble protein gene, the tissue-specific promoter is cut off for four times, and then the cut-off promoter fragment with an endosperm-specific promoter function as shown in SEQ ID NO.2-5 is obtained after molecular identification is carried out on the cut-off promoter fragment and an RUBY visual marker; the betaine content of the expression product can reach 2.5% of the dry weight of the seeds through determination, and the method has very high application value in the field of plant synthetic biology. The invention further discloses application of the promoter or the truncated promoter fragment in the aspects of improving crop seed quality, improving crop traits, cultivating new varieties of transgenic plants by using key elements of the promoter or expressing high value-added proteins by using a seed bioreactor and the like.

Provided are systems and methods for tissue specific modeling of post-transcriptional gene regulation (PTGR) using a neural network. The PTGR model operates to predict tissue- specific measures of mRNA stability such as miRNA binding and degradation. The systems and methods may use a dilated convolutional neural network for predicting mRNA stability based on miRNA binding and mRNA degradation in a sequence-to-sequence manner. Also provided are methods for generating PTGR models using miRNA binding data and mRNA degradome data.

The present invention relates to the field of plant genetic engineering technology, specifically providing a rice endosperm tissue-specific promoter, pPROLM26, and its applications. The present invention isolates a tissue-specific promoter with the nucleotide sequence shown in SEQ ID NO. 1 from a rice alcohol-soluble protein gene. This tissue-specific promoter is truncated four times and then molecularly identified with a RUBY visualization marker to obtain truncated promoter fragments shown in SEQ ID NOs. 2 to 5, each with endosperm-specific promoter function. The expression product has been measured to contain up to 2.5% betaine of the seed dry weight, demonstrating its high application value in the field of plant synthetic biology. The present invention further discloses applications of the promoter or truncated promoter fragments in improving crop seed quality, improving crop traits, cultivating new transgenic plant varieties using key elements of the promoter, or expressing high-value-added proteins using seed bioreactors.

A method for detecting the extent of microplastic accumulation in bivalve organisms in extreme deep-sea environments is provided. This method is performed through sampling of biological communities to represent the community structure of that biological bed layer, then morphological characterization statistics are conducted to classify the age stage data of individual bivalves, multivariate factor analysis is used to obtain the individual bivalves with the greatest degree of contribution in each classified age stage, subsequently tissue-specific microplastic extraction is performed, the morphology of seafloor microplastics is restored and streamlined identification of full-size microplastics is considered, carbon-14 dating is used to trace the duration of microplastic adsorption by each individual bivalve, dynamic accumulation curves for seafloor bivalves are constructed by connecting each bivalve's survival duration, and finally this is scaled up to the entire bivalve bed to derive the microplastic accumulation rate and historical accumulation of the entire extreme ecosystem.

The invention relates to the field of biological medicine, and particularly discloses a regulatory element combination for driving nucleic acid expression based on ITR-enhancer and application of the regulatory element combination. The regulatory element combination for driving nucleic acid expression based on an ITR-enhancer comprises at least one inverted terminal repeat (ITR) and at least one enhancer, the regulatory element combination is delivered through an AAV vector. The invention also provides a recombinant nucleic acid molecule for improving specific delivery of the target gene. The recombinant nucleic acid molecule comprises the regulatory element combination, an exogenous target gene and polyadenylic acid. The AAV vector provided by the invention can be used for driving the expression of a target gene without the action of a traditional promoter, and has the advantages of enhancer tissue specificity, short sequence (50-100bp), pathological microenvironment response and the like, thereby breaking through the capacity, targeting and dynamic limitation of the traditional AAV vector, improving the DNA (Deoxyribose Nucleic Acid) carrying capacity of the AAV vector, and simultaneously improving the expression of the target gene. The tissue and spatio-temporal expression specificity of a target gene is realized.

The invention discloses an application and a method of an overexpression transcription factor PtoLBD1.2 gene in promoting growth and development of populus tomentosa, a nucleotide sequence of the transcription factor PtoLBD1.2 gene is shown as SEQ ID NO.3. A transgenic plant is overexpressed through transgenic tissue specificity PtoLBD1.2, a plant with high expression quantity is screened out through subsequent positive identification for phenotypic observation, and a result shows that the transcription factor PtoLBD1.2 gene can be used as an overexpression transcription factor PtoLBD1.2 gene for promoting growth and development of populus tomentosa. Compared with the wild type in the same period, the transgenic plant of PtoLBD1.2 has the advantages that the plant height is increased, the stem is thickened, and the aboveground biomass is obviously improved. According to the cultivation method provided by the invention, the high-quality poplar variety with high growth speed and high biomass is cultivated. The method has great significance in molecular breeding and cultivation of excellent poplar in the future.

The invention discloses a genetic disease pathogenic site sorting and diagnosis auxiliary method and system based on multi-modal artificial intelligence. The method comprises the following steps: firstly, collecting genetic disease data to construct a heterogeneous knowledge graph; the method comprises the following steps: acquiring patient data, and executing an analysis process: generating multi-modal feature representation of candidate pathogenic sites from four dimensions of variation features based on rules, a tissue specificity mechanism, a protein three-dimensional structure and real-time literature evidence; carrying out fusion sorting on the features by using a sorting model, and generating an interpretable report and a clinical examination suggestion based on the uncertainty of a sorting result; and after the doctor executes examination according to the suggestion and feeds back newly added data, the analysis process is repeated until a preset iteration target is achieved. According to the method, the limitation of a static analysis model is broken through, multi-dimensional evidence fusion and clinical workflow embedding are realized, and the interpretation accuracy and diagnosis efficiency of the pathogenic site of the genetic disease are remarkably improved.

The invention discloses a mouse multi-gene collaborative insertion and knockout system based on multi-data coupling, and relates to the technical field of bioengineering, and the mouse multi-gene collaborative insertion and knockout system comprises a multi-gene collaborative targeting vector, a high-fidelity Cas9 protein expression unit, a homologous recombination template library, a multi-modal efficiency prediction module, a dual-fluorescence Cre report unit and an off-target inhibition element. According to the method, vector silencing is avoided by connecting the nuclear matrix attachment region in series with the gRNA expression cassette, and the multi-gene collaborative editing efficiency is improved to gt by combining homologous arm optimization design and a machine learning driven efficiency prediction model; 80%; cas9 fusion deaminase and a glycosylase inhibitor are combined with miRNA-mediated off-target inhibition, so that off-target sites of a whole genome are reduced to be less than or equal to 1 / sample; real-time monitoring of tissue specific expression is achieved by means of a double-fluorescence Cre reporting system, space-time regulation and control application is supported, and a high-reliability tool is provided for complex gene interaction research.

The invention belongs to the technical field of proteomics detection, and particularly relates to a method for detecting mouse liver neomembrane proteome based on an orthogonal translation system and a mouse strain containing an SORT-KASM module. Comprising the following steps: (S.1) constructing a conditionally expressed SORT-KASM transgenic mouse strain; (S.2) activating the expression of the SORT-KAS M by a tissue specific Cre system; (S.3) giving unnatural amino acid ingestion to the mouse; and (S.4) carrying out biotin labeling coupling on non-natural amino acids in the proteome through an azide-alkyne click reaction. According to the detection method, the space and time high resolution of the liver tissue is achieved, the membrane protein has the high-coverage marking capacity, the low-abundance newborn protein has the high-sensitivity marking capacity, good stability is achieved, and the membrane protein participates in the important physiological processes such as substance transfer, energy metabolism and steady state maintaining; the innovation of the detection method provides a new thought for researching the liver cell membrane proteome.

The present application provides an oligonucleotide conjugate and a pharmaceutical composition comprising the oligonucleotide conjugate. The oligonucleotide conjugate comprises a functional double-stranded oligonucleotide, a lipophilic moiety, and a peptide chain, wherein the functional double-stranded oligonucleotide, the lipophilic moiety, and the peptide chain are connected by means of linkers. The oligonucleotide conjugate and the pharmaceutical composition provided in the present application can effectively regulate the expression level of a target gene in vivo or in vitro, and have excellent tissue specificity. Therefore, the oligonucleotide conjugate and the pharmaceutical composition can effectively treat and / or prevent disease symptoms related to the level of mRNA expressed by the target gene. The present application holds promise.

The invention discloses application of a SbMYB113-like gene to improvement of low-temperature-resistant breeding of scutellaria baicalensis, belongs to the technical field of plant biology, is used for scutellaria baicalensis, and is characterized in that the nucleotide sequence of the SbMYB113-like gene is shown as SEQ ID NO: 1, by reducing overexpression of the SbMYB113-like gene in the scutellaria baicalensis, the anti-oxidation defense system of the scutellaria baicalensis is improved, the stability of the cell structure and function is protected, and the stability of the low-temperature-resistant breeding of the scutellaria baicalensis is improved. The tolerance of the scutellaria baicalensis to low-temperature stress is improved. The invention aims at researching subcellular localization of SbMYB113-like protein, analyzing tissue specific expression and a low-temperature stress response expression mode of the SbMYB113-like gene in combination with real-time fluorescent quantitative PCR (Polymerase Chain Reaction), and verifying the cold resistance function of SbMYB113-like through genetic transformation, so that the SbMYB113-like gene has an application value in low-temperature scutellaria baicalensis breeding.

The application relates to the field of biological medicine, in particular to application of TDF in serum cholesterol regulation and a verification method. The application discloses that tenofovir disoproxil fumarate (TDF) can inhibit intestinal cholesterol absorption by specifically down-regulating the expression level of NPC1L1 protein in the intestinal tract, so as to reduce serum cholesterol. The regulation has tissue specificity, and TDF has no significant influence on the expression of key genes of liver cholesterol metabolism. The application also provides a method for verifying the regulation of TDF on serum cholesterol, which is evaluated by detecting blood lipid indexes and the gene and / or protein expression level of NPC1L1 in duodenal tissue. The application provides a treatment scheme with antiviral and cholesterol-lowering effects for patients with chronic hepatitis B combined with hypercholesterolemia, and provides a new idea for developing a lipid-lowering strategy targeting intestinal cholesterol absorption.

The present application discloses an endogenous promoter of Koelreuteria paniculata or Koelreuteria paniculata var. variegata and its application in regulating plant leaf color development. GLK The present application compares the leaf phenotype difference of Koelreuteria paniculata and Koelreuteria paniculata var. variegata, analyzes the chloroplast structure of the two, and determines the photosynthetic characteristic index, finds that the net photosynthetic rate of Koelreuteria paniculata var. variegata is significantly lower than that of Koelreuteria paniculata, and accordingly infers that the gene expression amount of Koelreuteria paniculata var. variegata is down-regulated due to the variation of the gene promoter. GLK Based on the transcriptome data, specific primers are designed to obtain the gene and perform tissue specificity and time sequence development expression pattern analysis, and further, through the FPNI-PCR technology, two types of promoters, A type and B type, are cloned from Koelreuteria paniculata and Koelreuteria paniculata var. variegata, and it is found through analysis that the A type promoter is a promoter common to Koelreuteria paniculata and Koelreuteria paniculata var. variegata, and the B type promoter is a promoter specific to Koelreuteria paniculata; it is found through GUS activity detection that the B type promoter has higher driving activity than the A type promoter. The present application has application prospect in cultivating plant varieties with high photosynthetic efficiency or ornamental plant varieties.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

A mouse model with conditional knockout of a vitamin D-binding protein gene, and the use thereof. A method comprises the following steps: S1, performing flox modification on the mouse vitamin D-binding protein gene to obtain F0-generation positive mice having undergone the flox modification; S2, mating said F0-generation positive mice with wild type mice to obtain F1-generation positive mice with preserved flox modification; S3, hybridizing the F1-generation positive mice with tissue-specific Cre tool mice to obtain heterozygous mice, and mutually mating the heterozygous mice to obtain homozygous mice; and S4, selecting the obtained homozygous mice to undergo tamoxifen injection, so as to obtain mice with conditional knockout of the vitamin D-binding protein gene.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

The present invention discloses the application of overexpressing the auxin transporter PtoPIN1a gene in improving the biomass of Populus tomentosa. The nucleotide sequence of the auxin transporter PtoPIN1a gene is shown as SEQ ID No.1. By using genetic engineering methods, tissue-specific PtoPIN1a overexpressing transgenic plants are obtained after transforming wild-type Populus tomentosa by the Agrobacterium tumefaciens infection method, and plants with higher expression levels are screened out through subsequent positive identification for phenotypic observation. The results show that compared with the wild-type in the same period, the transgenic plants of PtoPIN1a have increased plant height, thicker stems, and significantly improved above-ground biomass. The cultivation technical method provided by the present invention has cultivated high-quality poplar varieties with high biomass. This invention has great significance for future molecular breeding of excellent poplars.

The invention discloses a nucleic acid molecule for inhibiting Zika virus, a pharmaceutical composition containing the nucleic acid molecule and application. The invention develops an anti-virus strategy for realizing the target host protein based on the RNA structure by changing the bottom-layer anti-virus logic. The MSI1 binding site in the nucleic acid molecule is highly conserved in all Zika virus strains, and the nucleic acid molecule has wide applicability. Meanwhile, the nucleic acid molecule can target an MSI1 high-expression region (namely virus-susceptible tissue), the problem of poor tissue specificity in existing delivery is solved, and the nucleic acid molecule is higher in targeting property, so that the nucleic acid molecule can cope with nervous system infection and pregnancy infection. In addition, the sequence design is relatively simple, and based on natural RNA sequence optimization, the modification demand is reduced, the natural affinity is high, and the chemical modification dependence is greatly reduced.

The invention discloses a DNA molecular marker, a primer and a detection method for bullfrog sex identification. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, and the nucleotide sequences of a primer pair for detecting the molecular marker are as shown in SEQ ID NO: 2 and SEQ ID NO: 3. The molecular marker provided by the invention meets the identification requirements of minimally invasive living bodies, is not influenced by the tissue specificity and development stage of bullfrogs, and is low in cost and simple to use; the molecular marker not only can quickly and efficiently identify the genetic sex of bullfrogs, but also can promote the establishment of bullfrog unisexual fry breeding technology, and has a wide application prospect.

Described herein are switchable core promoters that may selectively promote transcription initiation in the presence of an activated response element, such as an activated enhancer. These switchable core promoters may be paired with cell type- or cell state-specific response element to produce engineered promoters that selectively promote transcription of a payload sequence in a target cell type or target cell state. Also described herein are methods of using switchable core promoters and polynucleotides containing switchable core promoters to selectively express a payload in a target cell type or target cell state.

The invention discloses an engineered exosome for immunotherapy of lung cancer and a preparation method of the engineered exosome, particularly relates to a method for delivering synNotch-CAR mRNA (messenger Ribonucleic Acid) into a body through the exosome for in-situ modification of macrophages, and provides the engineered exosome capable of immunotherapy of lung cancer. The exosome derived from the M1 macrophages is used for engineering transformation, and targeted delivery of drugs to the macrophages is realized. The synNotch-CAR-TAMs in-vivo transformation strategy provided by the invention has high controllability, tissue specificity and durability, is expected to be expanded to various solid tumor types in the future, and provides a precise treatment scheme with higher clinical transformation value for cancer patients. The exosome is subjected to a series of engineering modification, so that the treatment effect of the exosome on lung cancer is improved.

This invention discloses a cSNP primer composition, kit, and its application for body fluid tracing based on next-generation sequencing technology, relating to the fields of forensic biological evidence and molecular identification technology. The cSNP primer composition includes primer pairs amplifying 34 cSNP genetic markers. The primer composition for body fluid tracing based on next-generation sequencing technology provided by this invention relates to 34 cSNP markers for identifying five types of body fluids. This invention has developed and established a kit capable of determining the body fluid type and genotyping the body fluid donor cSNPs. Using the primer composition or kit provided by this invention, highly consistent genotyping results can be obtained, with high sensitivity and good tissue specificity. It provides an effective detection tool for directly associating common body fluid types and body fluid donors, and is of great significance for further research on body fluid identification and achieving accurate and efficient forensic identification related to body fluids.

The invention provides a bifunctional protein degradation agent for directly targeting proteasome, and a preparation method and application thereof. The bifunctional protein degradation agent comprises a POI ligand for specifically binding to a to-be-degraded target protein related to a disease; the proteasome ligand is used for specifically binding a protein degradation tool proteasome; and a linker chain moiety linking the proteasome ligand to the POI ligand. Specifically, according to the degradation technology, degradation is achieved by directly collecting POIs near intracellular proteasomes. The invention establishes a novel targeted protein degradation technology platform, and provides a method for preparing the protein degradation agent at the same time. The invention also has the advantages that the compound molecule not only recruits POI to proteasome, but also can improve the hydrolytic activity of the proteasome and enhance the degradation efficiency of the POI. And the proteasome is highly expressed in all cells, and tissue specificity does not exist, so that the direct targeting proteasome degradation agent in the technology can be widely applied.

The present invention relates to an isolated peptide comprising an active fragment derived from an integrin alpha or beta subunit and capable of specific binding to the extracellular matrix of a target organ or a target cell or a target tissue in said target organ. The targeted delivery system constructed using the isolated peptide of the present invention exhibits enhanced tissue specificity, enabling precise delivery to the target organ, enhancing the therapeutic response by concentrating the therapeutic payload at the site of action, while reducing the required dosage and mitigating systemic side effects.

The invention relates to the technical field of plant breeding, in particular to a soybean leaf specific promoter Pad and application thereof. The invention provides a leaf-specific promoter Phad of soybean. The nucleotide sequence of the leaf-specific promoter Phad is as shown in SEQ ID NO: 1. The invention also provides a primer for amplifying the leaf specific promoter Pad, a recombinant expression vector containing the leaf specific promoter Pad, and a recombinant bacterium containing the leaf specific promoter Pad. The leaf specific promoter Phad can be specifically expressed in transgenic soybean plant leaf tissues, and the problem that soybean endogenous tissue specific regulation elements are deficient is solved.

The invention generally relates to methods for assessing the health of a tissue by characterizing circulating nucleic acids in a biological sample. According to certain embodiments, methods for assessing the health of a tissue include the steps of detecting a sample level of RNA in a biological sample, comparing the sample level of RNA to a reference level of RNA specific to the tissue, determining whether a difference exists between the sample level and the reference level, and characterizing the tissue as abnormal if a difference is detected.

The invention belongs to the technical field of gene editing, and particularly relates to a construction method of a transgenic mouse with a tissue-specific precancerous marker. A fertilized ovum is constructed through a gene editing technology, a mutant p53 protein and a fluorescent protein are fused, a loxp-Stop-loxp sequence is added in front of a promoter of a mutant p53 sequence, the fertilized ovum is transplanted into a female mouse, the obtained progeny is an LPL transgenic mouse, and expression inhibition of the mutant p53 protein and the fluorescent protein is achieved; the LPL transgenic mouse is mated with a Cre tool mouse to obtain a transgenic mouse of which the offspring is a tissue-specific precancerous marker, the transgenic mouse expresses mutant p53 protein and fluorescent protein at a specific tissue, early tumor focus development is monitored and traced through a living body fluorescence imaging system, and the LPL transgenic mouse has extremely high application and research values.

The application discloses a transcription factor AbHY5 for regulating the formation of colorful leaf color of Guzmania lingulata and application thereof. The application clones a light signal transcription factor AbHY5 from Guzmania lingulata, and the expression of the transcription factor has obvious tissue specificity and is specifically expressed in petals and red leaves. Research shows that the AbHY5 promoter responds to light induction, the expression level of AbHY5 significantly decreases under dark conditions, and the expression of AbHY5 has extremely significant positive correlation with the content of anthocyanin, flavonoids, sucrose and starch. Further research by transgenic technology shows that overexpression of AbHY5 significantly promotes the synthesis of pigments and flavonoids of the transgenic plant, the accumulation of endogenous sucrose and starch and the balance of ROS in the leaves, can increase the content of flavonoids, chlorophyll a, anthocyanin and carotenoids, and improve the photosynthetic rate of the plant leaves, thereby providing support for cultivating new Guzmania varieties with colorful leaves.

The application provides application of an IGFBP1 gene in regulation or evaluation of hen reproductive performance, and belongs to the field of biotechnology and genetic breeding. The application promotes expression of the IGFBP1 gene of a hen by constructing a tissue-specific IGFBP1 recombinant adeno-associated virus vector and using the tissue-specific IGFBP1 recombinant adeno-associated virus vector to promote expression of the IGFBP1 gene of the hen, and improves the IGFBP1 protein content in serum of the hen, and further improves the reproductive performance of the hen. The application proves by experiments that the IGFBP1 gene has an influence on the egg production performance of a hen and the expression level of an HPG axis hormone, can improve the total egg production of a hen, an ovary index, the number of small white follicles, the number of large white follicles, and the content of LH, E2, AMH and FSH in serum, can be used as a candidate gene or a genetic marker of egg production performance of a hen, enriches the serum judgment index of hen reproductive performance, and provides a valuable reference basis for differential diagnosis of hen reproductive performance.