32 results about "Tissue specificity" patented technology

A method for detecting the extent of microplastic accumulation in bivalve organisms in extreme deep-sea environments is provided. This method is performed through sampling of biological communities to represent the community structure of that biological bed layer, then morphological characterization statistics are conducted to classify the age stage data of individual bivalves, multivariate factor analysis is used to obtain the individual bivalves with the greatest degree of contribution in each classified age stage, subsequently tissue-specific microplastic extraction is performed, the morphology of seafloor microplastics is restored and streamlined identification of full-size microplastics is considered, carbon-14 dating is used to trace the duration of microplastic adsorption by each individual bivalve, dynamic accumulation curves for seafloor bivalves are constructed by connecting each bivalve's survival duration, and finally this is scaled up to the entire bivalve bed to derive the microplastic accumulation rate and historical accumulation of the entire extreme ecosystem.

The invention discloses a genetic disease pathogenic site sorting and diagnosis auxiliary method and system based on multi-modal artificial intelligence. The method comprises the following steps: firstly, collecting genetic disease data to construct a heterogeneous knowledge graph; the method comprises the following steps: acquiring patient data, and executing an analysis process: generating multi-modal feature representation of candidate pathogenic sites from four dimensions of variation features based on rules, a tissue specificity mechanism, a protein three-dimensional structure and real-time literature evidence; carrying out fusion sorting on the features by using a sorting model, and generating an interpretable report and a clinical examination suggestion based on the uncertainty of a sorting result; and after the doctor executes examination according to the suggestion and feeds back newly added data, the analysis process is repeated until a preset iteration target is achieved. According to the method, the limitation of a static analysis model is broken through, multi-dimensional evidence fusion and clinical workflow embedding are realized, and the interpretation accuracy and diagnosis efficiency of the pathogenic site of the genetic disease are remarkably improved.

The invention belongs to the technical field of proteomics detection, and particularly relates to a method for detecting mouse liver neomembrane proteome based on an orthogonal translation system and a mouse strain containing an SORT-KASM module. Comprising the following steps: (S.1) constructing a conditionally expressed SORT-KASM transgenic mouse strain; (S.2) activating the expression of the SORT-KAS M by a tissue specific Cre system; (S.3) giving unnatural amino acid ingestion to the mouse; and (S.4) carrying out biotin labeling coupling on non-natural amino acids in the proteome through an azide-alkyne click reaction. According to the detection method, the space and time high resolution of the liver tissue is achieved, the membrane protein has the high-coverage marking capacity, the low-abundance newborn protein has the high-sensitivity marking capacity, good stability is achieved, and the membrane protein participates in the important physiological processes such as substance transfer, energy metabolism and steady state maintaining; the innovation of the detection method provides a new thought for researching the liver cell membrane proteome.

The present application provides an oligonucleotide conjugate and a pharmaceutical composition comprising the oligonucleotide conjugate. The oligonucleotide conjugate comprises a functional double-stranded oligonucleotide, a lipophilic moiety, and a peptide chain, wherein the functional double-stranded oligonucleotide, the lipophilic moiety, and the peptide chain are connected by means of linkers. The oligonucleotide conjugate and the pharmaceutical composition provided in the present application can effectively regulate the expression level of a target gene in vivo or in vitro, and have excellent tissue specificity. Therefore, the oligonucleotide conjugate and the pharmaceutical composition can effectively treat and / or prevent disease symptoms related to the level of mRNA expressed by the target gene. The present application holds promise.

The invention discloses application of a SbMYB113-like gene to improvement of low-temperature-resistant breeding of scutellaria baicalensis, belongs to the technical field of plant biology, is used for scutellaria baicalensis, and is characterized in that the nucleotide sequence of the SbMYB113-like gene is shown as SEQ ID NO: 1, by reducing overexpression of the SbMYB113-like gene in the scutellaria baicalensis, the anti-oxidation defense system of the scutellaria baicalensis is improved, the stability of the cell structure and function is protected, and the stability of the low-temperature-resistant breeding of the scutellaria baicalensis is improved. The tolerance of the scutellaria baicalensis to low-temperature stress is improved. The invention aims at researching subcellular localization of SbMYB113-like protein, analyzing tissue specific expression and a low-temperature stress response expression mode of the SbMYB113-like gene in combination with real-time fluorescent quantitative PCR (Polymerase Chain Reaction), and verifying the cold resistance function of SbMYB113-like through genetic transformation, so that the SbMYB113-like gene has an application value in low-temperature scutellaria baicalensis breeding.

The application relates to the field of biological medicine, in particular to application of TDF in serum cholesterol regulation and a verification method. The application discloses that tenofovir disoproxil fumarate (TDF) can inhibit intestinal cholesterol absorption by specifically down-regulating the expression level of NPC1L1 protein in the intestinal tract, so as to reduce serum cholesterol. The regulation has tissue specificity, and TDF has no significant influence on the expression of key genes of liver cholesterol metabolism. The application also provides a method for verifying the regulation of TDF on serum cholesterol, which is evaluated by detecting blood lipid indexes and the gene and / or protein expression level of NPC1L1 in duodenal tissue. The application provides a treatment scheme with antiviral and cholesterol-lowering effects for patients with chronic hepatitis B combined with hypercholesterolemia, and provides a new idea for developing a lipid-lowering strategy targeting intestinal cholesterol absorption.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

The invention discloses a nucleic acid molecule for inhibiting Zika virus, a pharmaceutical composition containing the nucleic acid molecule and application. The invention develops an anti-virus strategy for realizing the target host protein based on the RNA structure by changing the bottom-layer anti-virus logic. The MSI1 binding site in the nucleic acid molecule is highly conserved in all Zika virus strains, and the nucleic acid molecule has wide applicability. Meanwhile, the nucleic acid molecule can target an MSI1 high-expression region (namely virus-susceptible tissue), the problem of poor tissue specificity in existing delivery is solved, and the nucleic acid molecule is higher in targeting property, so that the nucleic acid molecule can cope with nervous system infection and pregnancy infection. In addition, the sequence design is relatively simple, and based on natural RNA sequence optimization, the modification demand is reduced, the natural affinity is high, and the chemical modification dependence is greatly reduced.

The invention discloses a DNA molecular marker, a primer and a detection method for bullfrog sex identification. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, and the nucleotide sequences of a primer pair for detecting the molecular marker are as shown in SEQ ID NO: 2 and SEQ ID NO: 3. The molecular marker provided by the invention meets the identification requirements of minimally invasive living bodies, is not influenced by the tissue specificity and development stage of bullfrogs, and is low in cost and simple to use; the molecular marker not only can quickly and efficiently identify the genetic sex of bullfrogs, but also can promote the establishment of bullfrog unisexual fry breeding technology, and has a wide application prospect.

The invention provides a bifunctional protein degradation agent for directly targeting proteasome, and a preparation method and application thereof. The bifunctional protein degradation agent comprises a POI ligand for specifically binding to a to-be-degraded target protein related to a disease; the proteasome ligand is used for specifically binding a protein degradation tool proteasome; and a linker chain moiety linking the proteasome ligand to the POI ligand. Specifically, according to the degradation technology, degradation is achieved by directly collecting POIs near intracellular proteasomes. The invention establishes a novel targeted protein degradation technology platform, and provides a method for preparing the protein degradation agent at the same time. The invention also has the advantages that the compound molecule not only recruits POI to proteasome, but also can improve the hydrolytic activity of the proteasome and enhance the degradation efficiency of the POI. And the proteasome is highly expressed in all cells, and tissue specificity does not exist, so that the direct targeting proteasome degradation agent in the technology can be widely applied.

The present invention relates to an isolated peptide comprising an active fragment derived from an integrin alpha or beta subunit and capable of specific binding to the extracellular matrix of a target organ or a target cell or a target tissue in said target organ. The targeted delivery system constructed using the isolated peptide of the present invention exhibits enhanced tissue specificity, enabling precise delivery to the target organ, enhancing the therapeutic response by concentrating the therapeutic payload at the site of action, while reducing the required dosage and mitigating systemic side effects.

The invention generally relates to methods for assessing the health of a tissue by characterizing circulating nucleic acids in a biological sample. According to certain embodiments, methods for assessing the health of a tissue include the steps of detecting a sample level of RNA in a biological sample, comparing the sample level of RNA to a reference level of RNA specific to the tissue, determining whether a difference exists between the sample level and the reference level, and characterizing the tissue as abnormal if a difference is detected.

The application discloses a transcription factor AbHY5 for regulating the formation of colorful leaf color of Guzmania lingulata and application thereof. The application clones a light signal transcription factor AbHY5 from Guzmania lingulata, and the expression of the transcription factor has obvious tissue specificity and is specifically expressed in petals and red leaves. Research shows that the AbHY5 promoter responds to light induction, the expression level of AbHY5 significantly decreases under dark conditions, and the expression of AbHY5 has extremely significant positive correlation with the content of anthocyanin, flavonoids, sucrose and starch. Further research by transgenic technology shows that overexpression of AbHY5 significantly promotes the synthesis of pigments and flavonoids of the transgenic plant, the accumulation of endogenous sucrose and starch and the balance of ROS in the leaves, can increase the content of flavonoids, chlorophyll a, anthocyanin and carotenoids, and improve the photosynthetic rate of the plant leaves, thereby providing support for cultivating new Guzmania varieties with colorful leaves.

The invention belongs to the field of CRK gene family research, and discloses an identification and expression regulation analysis method of amorphophallus konjac AkCRKs gene family members, which is based on a hidden Markov model of a CRK gene conserved domain, and uses TBtools software to carry out identification and bioinformatics analysis on the AkCRKs gene family members. And the expression characteristics of AkCRKs gene family members in different tissue parts, jasmonic acid, soft rot bacteria (Pcc) and under low temperature stress are analyzed. 19 AkCRKs gene family members are identified in the whole genome of amorphophallus konjac and are non-uniformly distributed on 7 chromosomes. The promoter of the AkCRKs gene family member comprises a plurality of adversity stress response, hormone response, growth and development regulation and other cis-acting elements. The expression of the AkCRKs gene has tissue specificity and participates in response to Pcc, MeJA and low-temperature stress. The research result of the invention provides a basis for further researching the function and adversity stress response mechanism of the amorphophallus konjac AkCRKs gene.

The invention provides an AAV variant tissue specificity screening method, device and system and a storage medium, and relates to the technical field of bioengineering and gene therapy. The screening method comprises the following steps: constructing an AAV variant library; obtaining high-throughput sequencing data; processing the high-throughput sequencing data by using a change point detection algorithm, and filtering the sequencing data according to a noise filtering threshold value; and quantifying the expression levels of the AAV variants in different tissues by using a standardized scoring system to obtain evaluation scores, and screening out the AAV variants with tissue specificity based on the evaluation scores. According to the screening method provided by the invention, the accuracy of variant recognition is improved through a double bar code system, the noise is dynamically filtered by using a change point detection algorithm, false positive and omission caused by a fixed threshold are avoided, cross-sample comparability is realized by adopting a standardized scoring system, and the accuracy of variant recognition is improved. Therefore, the efficiency, the accuracy and the credibility of screening the tissue-specific AAV variants are remarkably improved.

Provided are a recombinant adeno-associated virus vector carrying the TYR gene, and the use thereof in the treatment of OCA1 disease. A recombinant adeno-associated virus capable of tissue-specific expression of the TYR protein is prepared by co-transfecting HEK293 cells with the REP protein of AAV, a CAP protein expression plasmid and a helper plasmid of a selected serotype, and the vector and then packaging same. The recombinant adeno-associated virus comprises an expression cassette of the human TYR gene, so that by means of suprachoroidal injection, the recombinant adeno-associated virus can effectively and targetedly infect the RPE and realize efficient and continuous tissue-specific expression of the TYR protein, thereby relieving disease-caused melanin deficiency, increasing the intensities of retinal electrophysiological a and b waves and achieving the purpose of treating oculocutaneous albinism type I (OCA1). In addition, the recombinant adeno-associated virus vector has the characteristics of simple and convenient administration, high safety and minimal induced inflammation.

The present application belongs to the technical field of bioinformatics, and particularly relates to a method for predicting poplar root-specific cis-regulatory elements and modules at the whole genome level. The present application discloses a method for predicting poplar root-specific cis-regulatory elements and modules at the whole genome level. For the first time, FIMO and tidymodels are combined to predict poplar tissue-specific cis-regulatory elements and modules at the whole genome level. Moreover, the promoter region is subjected to truncation analysis, and the TFBSs of the five promoter regions are respectively used to construct a random forest prediction model, so as to obtain TFBSs with high feature scores as candidate CREs. The candidate CREs are used to screen root-specific cis-regulatory modules, and experimental verification is performed. The method of the present application has the characteristics of rapidity and high throughput, and provides technical support for predicting plant root-specific cis-regulatory modules, and has practical value and wide application prospect.

Provided is the use of a recombinant adeno-associated virus expressing RS1 protein in the preparation of an anti-X-linked juvenile retinoschisis (XLRS) drug, belonging to the technical field of biomedicine. In order to fill the clinical research gap of recombinant adeno-associated viruses capable of efficient tissue-specific expression of RS1 protein for preventing and / or treating XLRS patients, the use of the recombinant adeno-associated virus expressing RS1 protein in the preparation of an anti-XLRS drug is provided. Clinical data indicates that the provided recombinant adeno-associated virus expressing RS1 protein can effectively improve the eyesight of XLRS patients, and can also significantly improve the retinal structure of XLRS patients, so that macular retinoschisis cavities can completely disappear. Moreover, safety data shows that there is no adverse events related to the drug itself, which indicates controllable safety. Thus, the drug is expected to become a new-generation clinical candidate drug for treating and preventing XLRS.

The invention provides a lotus embryo specific expression promoter pNnNF-YB4, a recombinant expression vector, recombinant bacteria and application, and belongs to the technical field of plant genetic engineering. According to the invention, a lotus embryo specific promoter pNnNF-YB4, which is located at upstream 1994bp of a specific high-expression gene NnNF-YB4 in cotyledons of lotus seeds, is found through gene sequencing. The promoter can drive a target gene to be highly expressed in a plant embryo, can regulate and control specific expression of other genes in the plant embryo by utilizing the expression characteristic of tissue specificity in the future so as to improve plant traits or cultivate new varieties of transgenic plants, and has good scientific research and commercial application prospects. The pNnNF-YB4 promoter disclosed by the invention is characterized in that the pNnNF-YB4 is used as a promoter, then a recombinant expression vector is constructed through Gateway reaction, wild-type arabidopsis thaliana is further converted to carry out promoter function verification, and a result shows that the pNnNF-YB4 promoter disclosed by the invention can specifically drive a downstream GUS gene to realize specific expression in arabidopsis thaliana seeds.

The application belongs to the technical field of biological medicine, and particularly relates to application of recombinant adeno-associated virus expressing RS1 protein in preparation of medicine for resisting X-linked juvenile retinoschisis (XLRS). In order to make up for the research blank of the recombinant adeno-associated virus expressing RS1 protein in prevention and / or treatment of XLRS patients in the clinic, the application provides application of the recombinant adeno-associated virus expressing RS1 protein in preparation of medicine for resisting XLRS. Clinical data shows that the recombinant adeno-associated virus expressing RS1 protein can effectively improve the vision of the XLRS patients, and can also obviously improve the retinal structure of the XLRS patients, so that the retinal split cavity in the macular region disappears completely; moreover, safety data shows that no adverse events related to the medicine itself are generated, the safety is controllable, and the recombinant adeno-associated virus expressing RS1 protein is expected to become a new generation of clinical candidate medicine for treating and preventing XLRS.

Embodiments disclosed herein are directed to engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the CRISPR complex to the binding site and / or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector protein is a Type V effector protein. In certain other example embodiments, the Type V effector protein is Cpf1. Embodiments disclosed herein are directed to viral vectors for delivery of CRISPR-Cas effector proteins, including Cpf1. In certain example embodiments, the vectors are designed so as to allow packaging of the CRISPR-Cas effector protein within a single vector. There is also an increased interest in the design of compact promoters for packing and thus expressing larger transgenes for targeted delivery and tissue-specificity. Thus, in another aspect certain embodiments disclosed herein are directed to delivery vectors, constructs, and methods of delivering larger genes for systemic delivery.

This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in male plant tissues, for example, to provide an economical male sterility system of hybrid seed production.

The invention relates to the technical field of plant genetic engineering, and particularly provides molecular cloning of a rice endosperm specific promoter pROLM24 and application of the rice endosperm specific promoter pROLM24. Three tissue-specific promoters with nucleotide sequences as shown in SEQ ID NO.1-3 are separated from a rice alcohol-soluble protein gene, the three tissue-specific promoters are fused with an RUBY visual marker, and a result shows that the content of betacyanin in an expression product is measured to be up to 2.9% of the dry weight of rice seeds. The method has very high application value in the field of plant synthetic biology. The invention further discloses application of the promoter in the aspects of improving crop seed quality, improving crop traits, cultivating new varieties of transgenic plants by using key elements of the promoter or expressing high value-added proteins by using a seed bioreactor and the like.

The invention belongs to the technical field of biology, and particularly relates to a mouse model for systemic expression or tissue specific expression of KRAS-G12D-EGFP fusion protein and a preparation method thereof, and the preparation method comprises the following steps: a) screening gRNA target sites; b) constructing a homologous recombinant vector; c) mixing the homologous recombinant vector, gRNA and Cas9 mRNA, injecting the mixture into a mouse fertilized egg, and transplanting the mixture into a mouse blastocyst; d) carrying out PCR (Polymerase Chain Reaction) screening on the F0-generation mice to obtain correct homologous recombination positive mice; and e) mating the positive F0-generation mouse with a wild type mouse, breeding, and carrying out PCR screening to obtain an F1-generation recombinant positive mouse. A mouse model modified by the gene is stable in heredity and can be used for researching the biological function of the KRAS-G12D mutant protein in a mouse body. Particularly, the mouse model can be used for researching the function of the KRAS-G12D mutant protein at the tissue specificity level, and the mutant protein can be subjected to cell tissue tracing by using EGFP fluorescent protein, so that the mouse model has a certain application value.

The application relates to the field of biological medicine, and particularly discloses a regulatory element combination based on ITR-enhancer-driven nucleic acid expression and application thereof. The regulatory element combination based on ITR-enhancer-driven nucleic acid expression comprises at least one inverted terminal repeat (ITR) and at least one enhancer; and the regulatory element combination is delivered through an AAV vector. The application also provides a recombinant nucleic acid molecule for improving target gene-specific delivery, which comprises the above regulatory element combination, an exogenous target gene and a poly A. The AAV vector provided by the application can drive the expression of a target gene without the action of a traditional promoter, and has the advantages of enhancer tissue specificity, a short sequence (50-100 bp) and pathological microenvironment response, thereby breaking through the limitations of traditional AAV vector capacity, targeting and dynamics, improving the DNA load of the AAV vector, and realizing the tissue and space-time expression specificity of the target gene.

The application discloses a molecular marker for identifying the genetic sex of a Zebra shark and a method for identifying male and female Zebra sharks, wherein the sequence of the molecular marker is shown as SEQ ID NO. 1. The method for identifying the genetic sex of the Zebra shark is simple and fast in operation, and a small amount of back fin is cut for sample collection, so that the Zebra shark is slightly damaged. An alkali lysis method is used for preparing a genome, and the DNA harvesting amount is high, and the requirement for a tissue sample is low. The gender identification molecular marker and the identification method provided by the application have high accuracy of identification results, are not affected by tissue specificity and environment, and have important values for Zebra shark gender control and all-male seed cultivation.

The invention discloses a function and an application of virus source tyrosine phosphatase in development of male testis of plutella xylostella, namely an application of a protein tyrosine phosphatase gene CvBV22-9 coded by cotesia vestalis virus in induction of apoptosis of testis cells of larvae of plutella xylostella. According to the invention, the CvBV22-9 gene is screened and identified as a main factor for inducing testis apoptosis, and the gene is continuously and highly expressed in a parasitized testis tissue and has tissue specificity. After the expression of the CvBV22-9 is knocked down, the testis apoptosis rate is obviously reduced; and about 90.80% of testis cell apoptosis can be induced by using recombinant baculovirus to over-express the gene. The low-concentration recombinant baculovirus can also destroy the testis structure and lead to decrease of the egg laying amount of mating female insects. According to the invention, a transgenic fruit fly model is successfully constructed, specific expression of CvBV22-9 in fruit fly testis is realized by using a UAS-Gal4 system, and 97.30% of testis apoptosis is induced.

This invention relates to the field of biotechnology and provides a gene-modified mouse model for tracking migratory organisms, its construction method, and its applications. The invention achieves precise labeling of endogenous migratory organisms by fusing the key structural protein TSPAN4 of the migratory organism with the fluorescent tag RFP and the modification tag Avi-tag, and site-specifically integrating it into the H11 site of mice, thus obtaining a TSPAN4-overexpressing transgenic mouse model. By introducing an LSL structure, the fusion gene is kept in a state of expression shutdown, achieving temporal and spatial specific activation only after mating with tissue-specific Cre tool mice. Multidimensional validation has shown that this model can accurately track migratory organisms in placental trophoblast cells, providing a standardized tool for assessing disease progression under physiological and pathological conditions through differences in migratory organism trajectory, inclusion packaging, and assembly, and can be directly applied to mechanistic studies and drug screening for diseases such as adverse pregnancy outcomes.

The application discloses a promoter with specific driving action and application thereof, and relates to the technical field of genetic engineering.The nucleotide sequence of the promoter provided by the application is shown in any one of SEQ ID NO.1-8.Eight novel tissue-specific promoters from Glycyrrhiza uralensis are successfully obtained, the promoters have clear and stable expression patterns and high driving activity, and can realize precise and directional expression of target genes in plant roots, stems, leaves and flower organs, and cover various types of multi-tissue coordinated expression.The promoters of the application are all endogenous sequences of plants, have stable driving activity and strong species adaptability, can construct specific expression cassettes and recombinant vectors, and are suitable for transformation of plants such as Arabidopsis thaliana and tobacco.The application enriches the resources of plant-specific promoters, solves the problems of single expression profile and insufficient precision of traditional promoters, and provides efficient, safe and diversified regulation tools for plant functional gene research, molecular breeding and improvement of medicinal plant traits.