250 results about "Tissue specificity" patented technology

A method of altering the targeting and/or cellular uptake efficiency of an adeno-associated virus (AAV) viral vector having a capsid containing an AAV9 cell surface binding domain is described. The method involves modifying a clade F cell surface receptor which comprises a glycan having a terminal sialic acid residue and a penultimate β-galactose residue. The modification may involve retargeting the vector by temporarily functionally ablate AAV9 binding in a subset of cells, thereby redirecting the vector to another subset of cells. Alternatively, the modification may involve increasing cellular update efficiency by treating the cells with a neuraminidase to expose cell surface β-galactose. Also provided are compositions containing the AAV9 vector and a neuraminidase. Also provided is a method for purifying AAV9 using β-galactose linked to solid support. Also provided are mutant vectors which have been modified to alter their targeting specificity, including mutant AAV9 in which the galactose binding domain is mutated and AAV in which an AAV9 galactose binding domain is engineered.

The present invention relates to methods for detecting free floating nucleic acids, as present in not cellular bound nucleic acids in bodily fluids like plasma or serum fractions of human or animal blood or in any other tissue samples derived from the human or animal body in order to diagnose a cell proliferative disease. Specifically the invention relates to the detection of increased levels of nucleic acids in bodily fluids. Furthermore the invention allows to determine the source of the enriched DNA by measuring the ratio of DNA originating from a certain organ versus total DNA from other organs in a given bodily fluid sample by specifying the DNA's methylation pattern. This can be done with or without increasing the DNA concentration of a given biological sample. In a preferred embodiment a further analysis of this methylation pattern allows for the detection of the presence of tumourous or otherwise proliferative disease in said organ.

The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin. inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.

The invention relates to methods for treating of tumor metastasis, methods for preventing, inhibiting, and predicting tumor recurrence and tumor metastasis, and methods of preventing cancer development for one at risk of developing cancer, for one diagnosed with a benign cancer, and/or for one diagnosed with malignant cancer. The invention also relates to methods of treating angiogenesis-dependent diseases and disorders. In addition, the invention provides: (1) a method for screening for tumor/cancer derived angiogenesis and metastasis factors; (2) a method for screening for compounds that inhibit angiogenesis and metastasis; (3) a method for screening for compounds that promote anti-angiogenesis and anti-metastasis activities; (4) a method for cancer prognosis evaluation; (5) a method for predicting the tissue specificity of a metastatic cancer; (6) a method for determining likelihood of metastasis; and (7) a method for cancer prognosis evaluation by the surveillance of metastasis development.

The present invention provides methods for the conditional or regulated expression of a site-specific recombinase using split intein-mediated protein splicing. This enhances the temporal and tissue-specificity of trait gene expression and allows for fine tuning of expression specificity.

The invention discloses a DNA fragment separating colony and functional checking of rice tissue special expressing promoter PD54O in plant gene engineering technical domain, which is characterized by the following: the PD54O promoter expresses in rice leaf and leaf sheath and does not express in germ and endosperm; transferring genetic rice plant can resist the invasion of borer with the expression of PD54O regulate and control insect-resisting gene; it does not express Bt protein in seeds of food.

The invention discloses HGG (Human Gammaglobulin) polypeptide in combination with the tissue specificity of cerebral arterial thrombosis. The invention relates to a method for obtaining the polypeptide. The method comprises the following steps: carrying out in-vivo selection of a mouse MCAO (Middle Cerebral Artery Occlusion) cerebral arterial thrombosis model by adopting an in-vivo phage display peptide library screening technology so as to obtain phage clones in combination with cerebral arterial thrombosis tissue; and randomly selecting the plurality of phage clones to sequence, and authenticating the in-vivo combination specificity of HGG peptide and coded phage clones HGG-M13 thereof. The invention further relates to application of the polypeptide in preparation of a high-sensitivity imaging molecular probe and a targeting delivery neuroprotective drug for cerebral stroke. The polypeptide can be synthesized through an artificial method; the polypeptide is low in molecular weight, high in activity and penetrating power, good in specificity and low in toxicity, and has good tissue targeting of cerebral arterial thrombosis in vivo; and therefore, the polypeptide is applicable to serving as a carrier of the high-sensitivity imaging molecular probe and the targeting delivery neuroprotective drug.

The invention relates to the technical field of identification of animal species and animal-derived components, in particular to a method for detecting a goose-derived component and a primer for detecting a nucleotide sequence of goose cell mitochondria, which aim to identify whether the goose-derived component is contained in products such as feeds, foods and the like or not. Due to the design of a specific amplification primer, mitochondrion deoxyribonucleic acid (DNA) which is independent of one another genetically, does not have repeated sequences and does not have tissue specificity in a biological individual is detected by using a polymerase chain reaction (PCR) technology, and a PCR detection method for the goose-derived component is established, so that the aim of quickly and accurately detecting and identifying the goose-derived component is fulfilled.

The invention discloses a Phytophthora nicotianae molecule detection primer and a detection method thereof, specially used for the specific molecule detection of Phytophthora nicotianae. A pair of specific primers of the Phytophthora nicotianae (comprising an upstream primer YhF:5'-GACATGATATCAACTGTTCTGCAG-3' and a downstream primer YhR:5'-CCTTGGATCTTCTCTCGATAAG-3') are mainly designed, and an amplified product having a fragment length of 342bp can be specially amplified on the pure DNA and bacteria bearing pathogenic tissues of the Phytophthora nicotianae through PCR amplification and agarose gel electrophoresis. The primer and the method can be used for the rapid, sensitive and specific detection of the Phytophthora nicotianae in Phytophthora nicotianae infected plant tissues in the production practices, can be used for the early stage diagnosis of field diseases and the monitoring and identification of pathogens, and provide reliable technologic and theoretic bases for the control of diseases caused by the Phytophthora nicotianae.

The invention discloses a tissue-specific gene and regulatory factor data storage method. In the method, data storage is realized by establishing a tissue-specific gene and regulatory factor database comprising a tissue bank, a gene bank, a gene reference name bank, a tissue-specific gene bank and the tissue bank of tissue-specific genes. The method comprises the following steps of: extracting the tissue-specific genes from a Pubmed bibliographic database in a literature mining form; adding extracted tissue information into the tissue bank; retrieving information about the genes in the European Molecular Biology Laboratory (EMBL), Genebank and NCBI by utilizing names of the genes, and adding the information into corresponding items of the gene bank; and generating regulatory factor XML files by utilizing the information of regulating the searching of the genes from Transfac, an Eukaryotic Promoter Database (EPD) and a compel database. Compared with the prior art, the method has the advantages of bringing convenience to researchers utilizing modern computing technology to detect gene expression and regulate an inherent mechanism of network tissue specificity to acquire data on sequences of the tissue-specific genes and corresponding regulatory factors, fully utilizing tissue-specific gene analysis tools and improving research quality and efficiency.

The invention discloses a gene PgPDR3 (panax ginseng pleiotropic drug resistance) related to promotion of transport and accumulation of ginsenosides, as well as an encoding protein and an application of the gene PgPDR3. A sequence of the PgPDR3 gene related to the transport and the accumulation of the ginsenosides is shown as SEQ ID No. (sequence identification number) 1; and the sequence of the encoding protein of the PgPDR3 gene is shown as SEQ ID No. 2. The research finds that PgPDR3 gene expression has tissue specificity and is closely related to the accumulation of the ginsenosides. The PgPDR3 gene has significant effects of promoting synthesis, the transport and the accumulation of the ginsenosides. Panax ginseng or other plants can be screened or improved through the PgPDR3 gene or derivatives of the PgPDR2 gene to obtain excellent plant varieties with high ginsenoside content. Also, panax ginseng transgenic cells or plants can be adopted to produce ginsenoside monomers such as protopanaxadiol, Rb, Re, Rh2, Rg2 and Rg3.

The invention discloses an HDT702 promoter and application thereof. The HDT702 promoter is obtained from cloning of rice, and the nucleotide sequence of the HDT702 promoter is shown in SEQ ID NO.1. The promoter is a tissue specificity promoter and can promote the specific expression of downstream genes in anther of rice. The HDT702 promoter is applied into the transgenic engineering, so that an expression product of target genes is specifically accumulated in the anther, and the local expression amount is increased; and meanwhile, unnecessary waste of plant nutrients can be avoided, and therefore good application prospects can be achieved in the transgenic engineering.

The invention discloses a method for positioning immune tissues of growth hormone for malus plants and application thereof. The method for positioning immune tissues of growth hormone for malus plants comprises the following steps of: (1) fixing plant tissues to obtain fixed plant tissues; (2) carrying out slice making on the fixed plant tissues on the basis of the step (1) to obtain a plant tissue slice; and (3) immunostaining the obtained plant tissue slice on the basis of the step (2), and determining the distribution condition of hormone in the plant tissues through observing a position of a positive signal. The method for positioning immune tissues of growth hormone for malus plants can eliminate the easily caused influence of nonspecific staining of woody plant tissues, and can identify the positive signal clearly. The method for positioning immune tissues of growth hormone for malus plants verifies that the growth hormone has tissue specificity when being distributed in the tissues of a stem tip or a root tip of an apple tree in a condition of iron deficiency.

The invention discloses a rice histone deacetylases gene HDT701 promoter and an application thereof. The sequence of the promoter is one of the following nucleotide sequences: (a) the nucleotide sequence shown in the sequence table SEQ ID NO.1; (b) the nucleotide sequence which can be hybridized with a DNA sequence of 1) under rigorous conditions and has same function; (c) the nucleotide sequencewhich has more than 90% homology with the DNA sequence of a) or b) and also has the function of the promoter. According to the invention, the promoter of the HDT701 gene is cloned from the rice, wherein the cloned promoter is the rice histone deacetylases gene HDT701 promoter which can promote the expression of downstream gene in some organs of the rice, and the rice histone deacetylases gene HDT701 promoter is a tissue specificity promoter, so that the promoter can be used in the transgenic engineering, the expression products of the target gene can be accumulated at certain organ or tissue so as to increase local expression quantity, and simultaneously, unnecessary waste of plant nutrient can be reduced, consequently, the promoter has good application prospect in the transgenic engineering.

The invention provides a molecular marker Marker Sex 1 and an identification method for quickly identifying genetic sex of portunus trituberculatus, wherein the Marker Sex 1 has the nucleotide sequence represented as the SEQ ID No.1. The identification method includes steps of: extracting genome DNA from muscle tissue of the portunus trituberculatus; with the genome DNA as a template, performing aPCR amplification reaction; purifying and sequencing the PCR amplification product; comparing the sequencing result with the DNA sequence of the molecular marker, and determining male if double peakexists or female if single peak exists. The identification method is simple and quick in operation and is low in demand on the sample, has high identification accuracy, is free of influence due to tissue specificity and environment, and has important effect for establishing all-female or high-female-ratio larval breeding technologies.