52 results about "Tissue specificity" patented technology

Provided are systems and methods for tissue specific modeling of post-transcriptional gene regulation (PTGR) using a neural network. The PTGR model operates to predict tissue- specific measures of mRNA stability such as miRNA binding and degradation. The systems and methods may use a dilated convolutional neural network for predicting mRNA stability based on miRNA binding and mRNA degradation in a sequence-to-sequence manner. Also provided are methods for generating PTGR models using miRNA binding data and mRNA degradome data.

The present invention relates to the field of plant genetic engineering technology, specifically providing a rice endosperm tissue-specific promoter, pPROLM26, and its applications. The present invention isolates a tissue-specific promoter with the nucleotide sequence shown in SEQ ID NO. 1 from a rice alcohol-soluble protein gene. This tissue-specific promoter is truncated four times and then molecularly identified with a RUBY visualization marker to obtain truncated promoter fragments shown in SEQ ID NOs. 2 to 5, each with endosperm-specific promoter function. The expression product has been measured to contain up to 2.5% betaine of the seed dry weight, demonstrating its high application value in the field of plant synthetic biology. The present invention further discloses applications of the promoter or truncated promoter fragments in improving crop seed quality, improving crop traits, cultivating new transgenic plant varieties using key elements of the promoter, or expressing high-value-added proteins using seed bioreactors.

A method for detecting the extent of microplastic accumulation in bivalve organisms in extreme deep-sea environments is provided. This method is performed through sampling of biological communities to represent the community structure of that biological bed layer, then morphological characterization statistics are conducted to classify the age stage data of individual bivalves, multivariate factor analysis is used to obtain the individual bivalves with the greatest degree of contribution in each classified age stage, subsequently tissue-specific microplastic extraction is performed, the morphology of seafloor microplastics is restored and streamlined identification of full-size microplastics is considered, carbon-14 dating is used to trace the duration of microplastic adsorption by each individual bivalve, dynamic accumulation curves for seafloor bivalves are constructed by connecting each bivalve's survival duration, and finally this is scaled up to the entire bivalve bed to derive the microplastic accumulation rate and historical accumulation of the entire extreme ecosystem.

The invention relates to the field of biological medicine, and particularly discloses a regulatory element combination for driving nucleic acid expression based on ITR-enhancer and application of the regulatory element combination. The regulatory element combination for driving nucleic acid expression based on an ITR-enhancer comprises at least one inverted terminal repeat (ITR) and at least one enhancer, the regulatory element combination is delivered through an AAV vector. The invention also provides a recombinant nucleic acid molecule for improving specific delivery of the target gene. The recombinant nucleic acid molecule comprises the regulatory element combination, an exogenous target gene and polyadenylic acid. The AAV vector provided by the invention can be used for driving the expression of a target gene without the action of a traditional promoter, and has the advantages of enhancer tissue specificity, short sequence (50-100bp), pathological microenvironment response and the like, thereby breaking through the capacity, targeting and dynamic limitation of the traditional AAV vector, improving the DNA (Deoxyribose Nucleic Acid) carrying capacity of the AAV vector, and simultaneously improving the expression of the target gene. The tissue and spatio-temporal expression specificity of a target gene is realized.

The invention discloses a genetic disease pathogenic site sorting and diagnosis auxiliary method and system based on multi-modal artificial intelligence. The method comprises the following steps: firstly, collecting genetic disease data to construct a heterogeneous knowledge graph; the method comprises the following steps: acquiring patient data, and executing an analysis process: generating multi-modal feature representation of candidate pathogenic sites from four dimensions of variation features based on rules, a tissue specificity mechanism, a protein three-dimensional structure and real-time literature evidence; carrying out fusion sorting on the features by using a sorting model, and generating an interpretable report and a clinical examination suggestion based on the uncertainty of a sorting result; and after the doctor executes examination according to the suggestion and feeds back newly added data, the analysis process is repeated until a preset iteration target is achieved. According to the method, the limitation of a static analysis model is broken through, multi-dimensional evidence fusion and clinical workflow embedding are realized, and the interpretation accuracy and diagnosis efficiency of the pathogenic site of the genetic disease are remarkably improved.

The invention discloses a mouse multi-gene collaborative insertion and knockout system based on multi-data coupling, and relates to the technical field of bioengineering, and the mouse multi-gene collaborative insertion and knockout system comprises a multi-gene collaborative targeting vector, a high-fidelity Cas9 protein expression unit, a homologous recombination template library, a multi-modal efficiency prediction module, a dual-fluorescence Cre report unit and an off-target inhibition element. According to the method, vector silencing is avoided by connecting the nuclear matrix attachment region in series with the gRNA expression cassette, and the multi-gene collaborative editing efficiency is improved to gt by combining homologous arm optimization design and a machine learning driven efficiency prediction model; 80%; cas9 fusion deaminase and a glycosylase inhibitor are combined with miRNA-mediated off-target inhibition, so that off-target sites of a whole genome are reduced to be less than or equal to 1 / sample; real-time monitoring of tissue specific expression is achieved by means of a double-fluorescence Cre reporting system, space-time regulation and control application is supported, and a high-reliability tool is provided for complex gene interaction research.

The invention belongs to the technical field of proteomics detection, and particularly relates to a method for detecting mouse liver neomembrane proteome based on an orthogonal translation system and a mouse strain containing an SORT-KASM module. Comprising the following steps: (S.1) constructing a conditionally expressed SORT-KASM transgenic mouse strain; (S.2) activating the expression of the SORT-KAS M by a tissue specific Cre system; (S.3) giving unnatural amino acid ingestion to the mouse; and (S.4) carrying out biotin labeling coupling on non-natural amino acids in the proteome through an azide-alkyne click reaction. According to the detection method, the space and time high resolution of the liver tissue is achieved, the membrane protein has the high-coverage marking capacity, the low-abundance newborn protein has the high-sensitivity marking capacity, good stability is achieved, and the membrane protein participates in the important physiological processes such as substance transfer, energy metabolism and steady state maintaining; the innovation of the detection method provides a new thought for researching the liver cell membrane proteome.

The present application provides an oligonucleotide conjugate and a pharmaceutical composition comprising the oligonucleotide conjugate. The oligonucleotide conjugate comprises a functional double-stranded oligonucleotide, a lipophilic moiety, and a peptide chain, wherein the functional double-stranded oligonucleotide, the lipophilic moiety, and the peptide chain are connected by means of linkers. The oligonucleotide conjugate and the pharmaceutical composition provided in the present application can effectively regulate the expression level of a target gene in vivo or in vitro, and have excellent tissue specificity. Therefore, the oligonucleotide conjugate and the pharmaceutical composition can effectively treat and / or prevent disease symptoms related to the level of mRNA expressed by the target gene. The present application holds promise.

The invention discloses application of a SbMYB113-like gene to improvement of low-temperature-resistant breeding of scutellaria baicalensis, belongs to the technical field of plant biology, is used for scutellaria baicalensis, and is characterized in that the nucleotide sequence of the SbMYB113-like gene is shown as SEQ ID NO: 1, by reducing overexpression of the SbMYB113-like gene in the scutellaria baicalensis, the anti-oxidation defense system of the scutellaria baicalensis is improved, the stability of the cell structure and function is protected, and the stability of the low-temperature-resistant breeding of the scutellaria baicalensis is improved. The tolerance of the scutellaria baicalensis to low-temperature stress is improved. The invention aims at researching subcellular localization of SbMYB113-like protein, analyzing tissue specific expression and a low-temperature stress response expression mode of the SbMYB113-like gene in combination with real-time fluorescent quantitative PCR (Polymerase Chain Reaction), and verifying the cold resistance function of SbMYB113-like through genetic transformation, so that the SbMYB113-like gene has an application value in low-temperature scutellaria baicalensis breeding.

The application relates to the field of biological medicine, in particular to application of TDF in serum cholesterol regulation and a verification method. The application discloses that tenofovir disoproxil fumarate (TDF) can inhibit intestinal cholesterol absorption by specifically down-regulating the expression level of NPC1L1 protein in the intestinal tract, so as to reduce serum cholesterol. The regulation has tissue specificity, and TDF has no significant influence on the expression of key genes of liver cholesterol metabolism. The application also provides a method for verifying the regulation of TDF on serum cholesterol, which is evaluated by detecting blood lipid indexes and the gene and / or protein expression level of NPC1L1 in duodenal tissue. The application provides a treatment scheme with antiviral and cholesterol-lowering effects for patients with chronic hepatitis B combined with hypercholesterolemia, and provides a new idea for developing a lipid-lowering strategy targeting intestinal cholesterol absorption.

The present application discloses an endogenous promoter of Koelreuteria paniculata or Koelreuteria paniculata var. variegata and its application in regulating plant leaf color development. GLK The present application compares the leaf phenotype difference of Koelreuteria paniculata and Koelreuteria paniculata var. variegata, analyzes the chloroplast structure of the two, and determines the photosynthetic characteristic index, finds that the net photosynthetic rate of Koelreuteria paniculata var. variegata is significantly lower than that of Koelreuteria paniculata, and accordingly infers that the gene expression amount of Koelreuteria paniculata var. variegata is down-regulated due to the variation of the gene promoter. GLK Based on the transcriptome data, specific primers are designed to obtain the gene and perform tissue specificity and time sequence development expression pattern analysis, and further, through the FPNI-PCR technology, two types of promoters, A type and B type, are cloned from Koelreuteria paniculata and Koelreuteria paniculata var. variegata, and it is found through analysis that the A type promoter is a promoter common to Koelreuteria paniculata and Koelreuteria paniculata var. variegata, and the B type promoter is a promoter specific to Koelreuteria paniculata; it is found through GUS activity detection that the B type promoter has higher driving activity than the A type promoter. The present application has application prospect in cultivating plant varieties with high photosynthetic efficiency or ornamental plant varieties.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

A mouse model with conditional knockout of a vitamin D-binding protein gene, and the use thereof. A method comprises the following steps: S1, performing flox modification on the mouse vitamin D-binding protein gene to obtain F0-generation positive mice having undergone the flox modification; S2, mating said F0-generation positive mice with wild type mice to obtain F1-generation positive mice with preserved flox modification; S3, hybridizing the F1-generation positive mice with tissue-specific Cre tool mice to obtain heterozygous mice, and mutually mating the heterozygous mice to obtain homozygous mice; and S4, selecting the obtained homozygous mice to undergo tamoxifen injection, so as to obtain mice with conditional knockout of the vitamin D-binding protein gene.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

The invention discloses a nucleic acid molecule for inhibiting Zika virus, a pharmaceutical composition containing the nucleic acid molecule and application. The invention develops an anti-virus strategy for realizing the target host protein based on the RNA structure by changing the bottom-layer anti-virus logic. The MSI1 binding site in the nucleic acid molecule is highly conserved in all Zika virus strains, and the nucleic acid molecule has wide applicability. Meanwhile, the nucleic acid molecule can target an MSI1 high-expression region (namely virus-susceptible tissue), the problem of poor tissue specificity in existing delivery is solved, and the nucleic acid molecule is higher in targeting property, so that the nucleic acid molecule can cope with nervous system infection and pregnancy infection. In addition, the sequence design is relatively simple, and based on natural RNA sequence optimization, the modification demand is reduced, the natural affinity is high, and the chemical modification dependence is greatly reduced.

The invention discloses a DNA molecular marker, a primer and a detection method for bullfrog sex identification. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1, and the nucleotide sequences of a primer pair for detecting the molecular marker are as shown in SEQ ID NO: 2 and SEQ ID NO: 3. The molecular marker provided by the invention meets the identification requirements of minimally invasive living bodies, is not influenced by the tissue specificity and development stage of bullfrogs, and is low in cost and simple to use; the molecular marker not only can quickly and efficiently identify the genetic sex of bullfrogs, but also can promote the establishment of bullfrog unisexual fry breeding technology, and has a wide application prospect.

This invention discloses a cSNP primer composition, kit, and its application for body fluid tracing based on next-generation sequencing technology, relating to the fields of forensic biological evidence and molecular identification technology. The cSNP primer composition includes primer pairs amplifying 34 cSNP genetic markers. The primer composition for body fluid tracing based on next-generation sequencing technology provided by this invention relates to 34 cSNP markers for identifying five types of body fluids. This invention has developed and established a kit capable of determining the body fluid type and genotyping the body fluid donor cSNPs. Using the primer composition or kit provided by this invention, highly consistent genotyping results can be obtained, with high sensitivity and good tissue specificity. It provides an effective detection tool for directly associating common body fluid types and body fluid donors, and is of great significance for further research on body fluid identification and achieving accurate and efficient forensic identification related to body fluids.

The invention provides a bifunctional protein degradation agent for directly targeting proteasome, and a preparation method and application thereof. The bifunctional protein degradation agent comprises a POI ligand for specifically binding to a to-be-degraded target protein related to a disease; the proteasome ligand is used for specifically binding a protein degradation tool proteasome; and a linker chain moiety linking the proteasome ligand to the POI ligand. Specifically, according to the degradation technology, degradation is achieved by directly collecting POIs near intracellular proteasomes. The invention establishes a novel targeted protein degradation technology platform, and provides a method for preparing the protein degradation agent at the same time. The invention also has the advantages that the compound molecule not only recruits POI to proteasome, but also can improve the hydrolytic activity of the proteasome and enhance the degradation efficiency of the POI. And the proteasome is highly expressed in all cells, and tissue specificity does not exist, so that the direct targeting proteasome degradation agent in the technology can be widely applied.

The present invention relates to an isolated peptide comprising an active fragment derived from an integrin alpha or beta subunit and capable of specific binding to the extracellular matrix of a target organ or a target cell or a target tissue in said target organ. The targeted delivery system constructed using the isolated peptide of the present invention exhibits enhanced tissue specificity, enabling precise delivery to the target organ, enhancing the therapeutic response by concentrating the therapeutic payload at the site of action, while reducing the required dosage and mitigating systemic side effects.

The invention generally relates to methods for assessing the health of a tissue by characterizing circulating nucleic acids in a biological sample. According to certain embodiments, methods for assessing the health of a tissue include the steps of detecting a sample level of RNA in a biological sample, comparing the sample level of RNA to a reference level of RNA specific to the tissue, determining whether a difference exists between the sample level and the reference level, and characterizing the tissue as abnormal if a difference is detected.

The invention belongs to the technical field of gene editing, and particularly relates to a construction method of a transgenic mouse with a tissue-specific precancerous marker. A fertilized ovum is constructed through a gene editing technology, a mutant p53 protein and a fluorescent protein are fused, a loxp-Stop-loxp sequence is added in front of a promoter of a mutant p53 sequence, the fertilized ovum is transplanted into a female mouse, the obtained progeny is an LPL transgenic mouse, and expression inhibition of the mutant p53 protein and the fluorescent protein is achieved; the LPL transgenic mouse is mated with a Cre tool mouse to obtain a transgenic mouse of which the offspring is a tissue-specific precancerous marker, the transgenic mouse expresses mutant p53 protein and fluorescent protein at a specific tissue, early tumor focus development is monitored and traced through a living body fluorescence imaging system, and the LPL transgenic mouse has extremely high application and research values.

The application discloses a transcription factor AbHY5 for regulating the formation of colorful leaf color of Guzmania lingulata and application thereof. The application clones a light signal transcription factor AbHY5 from Guzmania lingulata, and the expression of the transcription factor has obvious tissue specificity and is specifically expressed in petals and red leaves. Research shows that the AbHY5 promoter responds to light induction, the expression level of AbHY5 significantly decreases under dark conditions, and the expression of AbHY5 has extremely significant positive correlation with the content of anthocyanin, flavonoids, sucrose and starch. Further research by transgenic technology shows that overexpression of AbHY5 significantly promotes the synthesis of pigments and flavonoids of the transgenic plant, the accumulation of endogenous sucrose and starch and the balance of ROS in the leaves, can increase the content of flavonoids, chlorophyll a, anthocyanin and carotenoids, and improve the photosynthetic rate of the plant leaves, thereby providing support for cultivating new Guzmania varieties with colorful leaves.

The application provides application of an IGFBP1 gene in regulation or evaluation of hen reproductive performance, and belongs to the field of biotechnology and genetic breeding. The application promotes expression of the IGFBP1 gene of a hen by constructing a tissue-specific IGFBP1 recombinant adeno-associated virus vector and using the tissue-specific IGFBP1 recombinant adeno-associated virus vector to promote expression of the IGFBP1 gene of the hen, and improves the IGFBP1 protein content in serum of the hen, and further improves the reproductive performance of the hen. The application proves by experiments that the IGFBP1 gene has an influence on the egg production performance of a hen and the expression level of an HPG axis hormone, can improve the total egg production of a hen, an ovary index, the number of small white follicles, the number of large white follicles, and the content of LH, E2, AMH and FSH in serum, can be used as a candidate gene or a genetic marker of egg production performance of a hen, enriches the serum judgment index of hen reproductive performance, and provides a valuable reference basis for differential diagnosis of hen reproductive performance.

The invention belongs to the field of CRK gene family research, and discloses an identification and expression regulation analysis method of amorphophallus konjac AkCRKs gene family members, which is based on a hidden Markov model of a CRK gene conserved domain, and uses TBtools software to carry out identification and bioinformatics analysis on the AkCRKs gene family members. And the expression characteristics of AkCRKs gene family members in different tissue parts, jasmonic acid, soft rot bacteria (Pcc) and under low temperature stress are analyzed. 19 AkCRKs gene family members are identified in the whole genome of amorphophallus konjac and are non-uniformly distributed on 7 chromosomes. The promoter of the AkCRKs gene family member comprises a plurality of adversity stress response, hormone response, growth and development regulation and other cis-acting elements. The expression of the AkCRKs gene has tissue specificity and participates in response to Pcc, MeJA and low-temperature stress. The research result of the invention provides a basis for further researching the function and adversity stress response mechanism of the amorphophallus konjac AkCRKs gene.

The invention provides an AAV variant tissue specificity screening method, device and system and a storage medium, and relates to the technical field of bioengineering and gene therapy. The screening method comprises the following steps: constructing an AAV variant library; obtaining high-throughput sequencing data; processing the high-throughput sequencing data by using a change point detection algorithm, and filtering the sequencing data according to a noise filtering threshold value; and quantifying the expression levels of the AAV variants in different tissues by using a standardized scoring system to obtain evaluation scores, and screening out the AAV variants with tissue specificity based on the evaluation scores. According to the screening method provided by the invention, the accuracy of variant recognition is improved through a double bar code system, the noise is dynamically filtered by using a change point detection algorithm, false positive and omission caused by a fixed threshold are avoided, cross-sample comparability is realized by adopting a standardized scoring system, and the accuracy of variant recognition is improved. Therefore, the efficiency, the accuracy and the credibility of screening the tissue-specific AAV variants are remarkably improved.

Provided are a recombinant adeno-associated virus vector carrying the TYR gene, and the use thereof in the treatment of OCA1 disease. A recombinant adeno-associated virus capable of tissue-specific expression of the TYR protein is prepared by co-transfecting HEK293 cells with the REP protein of AAV, a CAP protein expression plasmid and a helper plasmid of a selected serotype, and the vector and then packaging same. The recombinant adeno-associated virus comprises an expression cassette of the human TYR gene, so that by means of suprachoroidal injection, the recombinant adeno-associated virus can effectively and targetedly infect the RPE and realize efficient and continuous tissue-specific expression of the TYR protein, thereby relieving disease-caused melanin deficiency, increasing the intensities of retinal electrophysiological a and b waves and achieving the purpose of treating oculocutaneous albinism type I (OCA1). In addition, the recombinant adeno-associated virus vector has the characteristics of simple and convenient administration, high safety and minimal induced inflammation.

The present application belongs to the technical field of bioinformatics, and particularly relates to a method for predicting poplar root-specific cis-regulatory elements and modules at the whole genome level. The present application discloses a method for predicting poplar root-specific cis-regulatory elements and modules at the whole genome level. For the first time, FIMO and tidymodels are combined to predict poplar tissue-specific cis-regulatory elements and modules at the whole genome level. Moreover, the promoter region is subjected to truncation analysis, and the TFBSs of the five promoter regions are respectively used to construct a random forest prediction model, so as to obtain TFBSs with high feature scores as candidate CREs. The candidate CREs are used to screen root-specific cis-regulatory modules, and experimental verification is performed. The method of the present application has the characteristics of rapidity and high throughput, and provides technical support for predicting plant root-specific cis-regulatory modules, and has practical value and wide application prospect.

Provided is the use of a recombinant adeno-associated virus expressing RS1 protein in the preparation of an anti-X-linked juvenile retinoschisis (XLRS) drug, belonging to the technical field of biomedicine. In order to fill the clinical research gap of recombinant adeno-associated viruses capable of efficient tissue-specific expression of RS1 protein for preventing and / or treating XLRS patients, the use of the recombinant adeno-associated virus expressing RS1 protein in the preparation of an anti-XLRS drug is provided. Clinical data indicates that the provided recombinant adeno-associated virus expressing RS1 protein can effectively improve the eyesight of XLRS patients, and can also significantly improve the retinal structure of XLRS patients, so that macular retinoschisis cavities can completely disappear. Moreover, safety data shows that there is no adverse events related to the drug itself, which indicates controllable safety. Thus, the drug is expected to become a new-generation clinical candidate drug for treating and preventing XLRS.

The invention provides a lotus embryo specific expression promoter pNnNF-YB4, a recombinant expression vector, recombinant bacteria and application, and belongs to the technical field of plant genetic engineering. According to the invention, a lotus embryo specific promoter pNnNF-YB4, which is located at upstream 1994bp of a specific high-expression gene NnNF-YB4 in cotyledons of lotus seeds, is found through gene sequencing. The promoter can drive a target gene to be highly expressed in a plant embryo, can regulate and control specific expression of other genes in the plant embryo by utilizing the expression characteristic of tissue specificity in the future so as to improve plant traits or cultivate new varieties of transgenic plants, and has good scientific research and commercial application prospects. The pNnNF-YB4 promoter disclosed by the invention is characterized in that the pNnNF-YB4 is used as a promoter, then a recombinant expression vector is constructed through Gateway reaction, wild-type arabidopsis thaliana is further converted to carry out promoter function verification, and a result shows that the pNnNF-YB4 promoter disclosed by the invention can specifically drive a downstream GUS gene to realize specific expression in arabidopsis thaliana seeds.

The invention relates to a populus tomentosa PtoYABBY2 gene and application thereof, and belongs to the field of plant genetic engineering. The nucleotide sequence of the gene is as shown in Seq ID NO.1, and the gene encodes a protein of Seq ID NO.2. The gene can obviously advance the flowering time of plants by regulating and controlling key factors (such as up-regulation of AtFT and the like and inhibition of AtFLC) of a flowering pathway. Through overexpression of the PtoYABBY2 in arabidopsis thaliana, the flowering time can be advanced by about 4 days on average, and floral organs grow normally. The gene shows tissue specific expression (mature leaves > young leaves > stems / roots) and dynamic expression in a flower bud development stage in populus tomentosa. The invention provides a construction method of a cloning primer, a fluorescent quantitative primer and an overexpression vector. The invention also discloses a method for cultivating populus tomentosa varieties with different flowering time by using the gene. By applying the gene, the breeding cycle of populus tomentosa can be shortened, the problems of long juvenile phase and flying catkins pollution are solved, and a new tool is provided for forest molecular design breeding.