180 results about "Multiple tumors" patented technology

The invention discloses a method for preparation of an addressable electrochemical transducer array, and determination multiple tumor markers in a sample by adopting the addressable electrochemical transducer array. The method comprises the following steps: printing a row electrode and a line electrode on a proper substrate material by preparing proper ink; selecting a proper paper material, preparing a reaction hydrophobic region and a hydrophilic region on the paper by utilizing a wax printing technology, and constructing a reference electrode and a counter electrode shared by an array electrode on the other paper-based material through a screen printing technology; functionalizing the prepared hydrophilic region, identifying an antigen, and marking a signal molecule; assembling the prepared working electrode, the reference electrode and the counter electrode with the prepared reaction region, and constructing the addressable electrochemical transducer array; and dripping an enzyme substrate in the reaction region, and adding phosphoric acid buffer liquid on the paper layer printed with the reference electrode and the counter electrode, and detecting the target.

The present invention encompasses methods and compositions for the generation and use of cytotoxic T lymphocytes that target multiple viruses or that are specific for multiple tumor antigens. In specific embodiments, the generation methods employ use of certain cytokines to promote proliferation and reduce cell death in an activated T cell population and / or that employ a particular bioreactor having a gas permeable membrane.

The invention relates to a kit for auxiliary diagnosis of multiple tumors by taking a micro ribonucleic acid (RNA) combination as a tumor marker, and a detection method of the kit. The invention belongs to the fields of genetic engineering and oncology. The detection method comprises the steps of (1) extracting RNA from serum; (2) carrying out polyadenylation on the 3' end of micro RNA; (3) purifying and recovering a tailing product; (4) carrying out an inverse transcription reaction on the tailing product; (5) testing the relative expression of the micro RNA by means of a fluorescent quantitative PCR (polymerase chain reaction); (6) analyzing the result of the fluorescent quantitative PCR. The kit has the characteristics of high conservative property, space-time specificity, stability, tissue specificity and the like, and plays an important role in the aspects such as disease pathogenesis research, early diagnosis, individualized treatment and prognosis; furthermore, the kit is simple to operate, convenient in material obtaining, safe and noninvasive, and is conveniently used for screening of a great deal of diseases.

The invention discloses a preparation method and application of a non-enzymatic electrochemical immunosensor based on dopamine biomemetic modification and relates to the fields of nano-science, biological immune technology, electrochemical sensing and the like. An electrode is functionalized by utilizing dopamine biomemetic modification, and platinum, silver, palladium and other metal nanoparticles with enzymatic activity are synthesized in situ by utilizing the reduction characteristic of a poly-dopamine modification layer; an antibody is immobilized on the surface of the electrode by means of the interaction of the metal nanoparticles and poly-dopamine modification layer; electrochemical immunoassay on a tumor marker can be realized based on the electrocatalytic effect of the metal nanoparticles to an H2O2 reduction reaction and the identification effect of the antibody molecule to an antigen. The method is simple in steps, easy to operate and low in cost, has a high electrochemical activity and excellent response performance, can solve the problem of difficult nano-material synthesis in a conventional method and lack of simple and effective method, is applicable to preparation of multiple tumor marker immunosensor electrodes, and has a wide application prospect in scientific research and clinical application.

The invention provides a compound with the structure shown in a formula I, a preparation method of the compound, and an application of the compound as a tyrosine kinase and / or serine-threonine kinase inhibitor. The compound has a good inhibitory action on the activity of multiple kinases, and has an obvious inhibitory effect on tyrosine kinase and / or serine-threonine kinase in biochemical level and cell level in vitro (P is less than 0.05), and the half inhibitory concentration (IC50) for c-Met kinase is commonly below 10<-6>mol / L; and the compound also has an obvious inhibitory effect on proliferation of multiple tumor cells (P is less than 0.05), and the IC50 is respectively below 10<-5>mol / L. The compound with the structure shown in the formula I can be applied to preparing medicines for treating diseases related to protein kinase in organisms. The formula I is shown in the specification.

A combined device for rapidly and quantitatively detecting multiple tumor markers is characterized by being composed of 3 to 8 pieces of independent tumor marker test paper such as AFP, CEA, PSA, CA125, CA153, CA199, CA242 and NSE, a clamping shell special for the 3 to 8 pieces of test paper, and an immune layer result analysis and judgment recorder with built-in corresponding standard curves and the judgment method. The test paper can be combined to form the detection device with the 3 to 8 pieces of test paper according to the specific requirements. The standard curves and the judgment method for the 8 pieces of test paper are built in the immune layer result analysis and judgment recorder and used for conducting quantitative judgment on test results, and forecasting of various tumor occurrence conditions can be conducted on different types of combination of the test results through the judgment method. Detection is completed within 15 minutes to 20 minutes. The combined device for rapidly and quantitatively detecting the multiple tumor markers has the advantages of being combined in detection, easy and convenient to operate, rapid in response, accurate in result, suitable for on-site test and the like, and the requirements for more accurate, easier and move convenient clinical detection are met.

The present invention encompasses methods and compositions for the generation and use of cytotoxic T lymphocytes that target multiple viruses or that are specific for multiple tumor antigens. In specific embodiments, the generation methods employ use of certain cytokines to promote proliferation and reduce cell death in an activated T cell population and / or that employ a particular bioreactor having a gas permeable membrane.

The invention belongs to the field of medicinal chemistry and discloses pyrimidotriazole compounds containing hydrazine bonds as well as a preparation method and an application of the pyrimidotriazole compounds in drug preparation. The general formula I of the compounds is shown in the specification. The compounds have remarkable inhibition and killing functions on multiple tumor cells such as MGC-803, MCF-7 and EC-109, can serve as candidate or lead compounds for further development and are applied to preparation of an anti-tumor drug.

The invention discloses a multi-targeting fusion cell membrane modified bionic nano delivery system as well as a preparation method and application thereof. The multi-targeting fusion cell membrane modified bionic nano delivery system comprises a fusion cell membrane and a negative electricity nano core wrapped by the fusion cell membrane, wherein the fusion cell membrane is obtained by fusing a macrophage membrane and a cancer cell membrane; and the negative electricity nano core carries a hydrophobic drug. The surface of the fusion cell membrane contains proteins on the surfaces of a macrophage membrane and a cancer cell membrane, so that the fusion cell membrane has a targeted adhesion recognition function, and a modified nano drug loading system is endowed with a multiple tumor targeting function. The fusion cell membrane modified bionic nano delivery system can deliver chemotherapeutic drugs, has the characteristics of good stability, low immunogenicity and good biocompatibility,and provides an effective means for hydrophobic therapeutic drug delivery in tumor targeted therapy.

The invention discloses a 4-aromatic aminoquinazoline derivative and a purpose thereof, wherein the 4-aromatic aminoquinazoline derivative has a structure shown as the formula (I). Pharmacological experiments indicate that the compounds or the pharmaceutically acceptable salts thereof can restrain the multiplication of multiple tumor cells. In the formula, ring A is a benzene ring, an alkylbenzene ring, a benzothiophene ring or an alkyl benzothiophene ring, R1 is hydrogen or alkoxy, n is an integer selected from 1 to 6, R2 is -NR4R5. R4 or R5 is respectively selected from hydrogen, alkyl and naphthenic base, or R4 and R5 are combined to form a substituted or unsubstituted heterolipid ring group, wherein the substituted group is an alkyl or hydroxyalkyl. R3 is an ester group, a substituted or unsubstituted aniline, substituted or unsubstituted pyridine amino, substituted or unsubstituted pyrimidine amino, substituted or unsubstituted benzyl carbamido, substituted or unsubstituted picolyl carbamido, substituted or unsubstituted pyrimidine methyl carbamido or substituted or unsubstituted naphthenic carbamido, wherein the substituted group is halogen, alkyl, phenyl or pyridyl or pyrimidyl, and preferably, the substituted group is phenyl, pyridyl or pyrimidyl.

A 4'-demethylepipodophyllotoxin derivative IIIa-n is prepared by taking etoposide as raw material, reacting with sodium iodide to generate 4-bit iodine substitute and obtain intermediate II, agitating while adding into barium carbonate, regulating reactive system pH 7-8, adding into amino-compound, and reacting to room temperature for 8-10 hrs to obtain final product. It's simple and efficient, it can avoid 4-bit surface isomerization and 4-bit demethylation, it has strong inhibiting function of multiple tumor cell strain and non-toxic.

The invention discloses gem-difluoro ethyl substitutional diphenylethene and diphenylethene diphenylethane derivatives as well as a preparation method and application thereof. A 4' bit of a B aromatic ring of diphenylethane / diphenylethene is subjected to chemical structure modification through gem-difluoro ethyl, and meanwhile, a 2' bit is modified into nitro or an amino group and other substituent groups; the gem-difluoro ethyl substitutional diphenylethene and diphenylethane derivative has relatively high antitumor activity in vitro, and the antitumor activity of a trans-form is better than the antitumor activity of a cis-form; with the introduction of fluorine atoms, not only is the physical property of the compound changed, but also the antitumor activity in vitro is improved at the same time, and the derivative has a relatively good inhibiting effect on multiple tumor cells.

OX40 is a potent immune stimulating target. Provided herein is a method of treating cancer, which includes administering to a subject in need of treatment an OX40 agonist and a common gamma chain (yc) cytokine or an active fragment, variant, analog, or derivative thereof. In certain aspects the common gamma chain (yc) cytokine is interleukin-2 (IL-2) or an active fragment, variant, analog, or derivative thereof. Combined treatment with an agonist anti-OX40 mAb and IL-2 synergized to augment tumor immunotherapy against multiple tumor types. Dual therapy was also able to restore the function of anergic tumor-reactive CD8+ T cells.

The invention relates to a double-specific chimeric antigen receptor T cell as well as a preparation method and application thereof. The double-specific chimeric antigen receptor double-specifically targeting human NKG2DL and human CD47 is constructed on the basis of human NKG2D and human SIRPalpha molecules as well as an immune response cell modified with the double-specific chimeric antigen receptor. The novel modified immune response cell can effectively realize targeting attack of multiple tumor cells, particularly positive tumor cells expressing NKG2DL and CD47 and can be used for preparing preparations for treating tumor. The preparation method of the immune response cell modified with the double-specific chimeric antigen receptor double-specifically targeting NKG2DL and CD47 comprises simple steps, the obtained immune response cell modified with the double-specific chimeric antigen receptor double-specifically targeting NKG2DL and CD47 has high specific killing rate for tumor cells.

The invention discloses a folic acid and polydopamine modified tumor-targeted binary drug loading nanoparticle with pH response as well as a preparation method and an application thereof. The preparation method comprises the following steps: (1) preparing hydrophobic drug loading nanoparticles; (2) modification of polydopamine; (3) modification of targeted ligand folic acid; and (4) a binary drug loading process of the pH response. The method disclosed by the invention is simple and wide in adaptability, and the prepared nanoparticle has good biocompatibility, biodegradability and target properties of multiple tumors; and due to the binary drug loading and pH response characteristics, the nanoparticle has an excellent treatment effect.

The invention discloses cell CTL (BiAT) with a bispecific antibody as well as a preparation method and an application thereof. The cytotoxicity T lymphocyte (CTL) with the bispecific antibody is prepared by assembling CTL cells by combining a CD3 monoclonal antibody and any antibody aiming at tumor-associated antigens (TAAs), and the CTL is named as CTL (BiAT) cells. The target binding of targeting specificity CTL and one tumor-associated antigen or more tumor-associated antigens is generated due to combined use of multiple tumor-associated antigen epitope peptides to stimulate DC induction, the targeting action of the CTL cells is ensured due to dual mechanisms, the number of the targets acting on the CTL cells is increased, the targeting property of the CTL cellular immune effect is further improved, and tumor cells can be efficiently killed.

The present invention encompasses methods and compositions for the generation and use of cytotoxic T lymphocytes that target multiple viruses or that are specific for multiple tumor antigens. In specific embodiments, the generation methods employ use of certain cytokines to promote proliferation and reduce cell death in an activated T cell population and / or that employ a particular bioreactor having a gas permeable membrane.

The invention discloses a multi-tumor-marker detecting platform based on a SERS detecting technology and a micro-fluidic chip. A SERS substrate is formed in a micro-fluidic channel at first to improve detection sensitivity, and then a typical sandwich structure is formed on the SERS substrate to detect whether a to-be-detected reagent contains specific tumor markers or not. The substrate is connected with a specific capturing antibody by a PDMS chip at first, and then a placing position of a new PDMS chip is perpendicular to a previous placing position to achieve the effect of simultaneously detecting multiple tumor markers in an intersection region. A SERS immunoprobe which is connected with a second antibody and a Raman reporting molecule to achieve the effect of qualitatively and quantitatively detecting the tumor marks in the to-be-detected reagent, the second antibody can amplify signals, and therefore, detection sensitivity can be improved further. The whole platform can simultaneously detect the multiple tumor markers qualitatively and quantitatively, and provides favorable technical support for early diagnosis of cancer of cancerous persons and increasing of survival rate.

The invention discloses new application of a 2-(4-fluorophenyl)-3-nitro-8-O-ethyl-2-hydro-benzopyran compound, in particular application of the compound serving as phosphatidylinositol-3-kinase (PI3K) inhibitor. The PI3K inhibitor can inhibit PI3K and disturb a PI3K / AKT signal channel, has good treatment effect on multiple tumors, particularly malignant hematological diseases, can effectively control the propagation of tumor cells and induce the apoptosis thereof, and has the effect of inhibiting and treating the tumors; and meanwhile, the PI3K has low toxicity.

The invention belongs to the field of medicinal chemistry, and discloses a class of pyrimidotriazole compounds, a preparation method thereof and the use of lysine-specific demethylase (hereinafter referred to as LSD1) as a target in the preparation of antitumor drugs Applications. The general formula of the compound of the present invention is shown in I. The LSD1 enzyme inhibitory activity experiment in vitro proves that this type of compound has obvious inhibitory and killing effects on a variety of tumor cells by inhibiting the activity of LSD1, and can be used as a lead compound for further development and applied to the preparation of anti-tumor drugs.

The invention discloses 4-aminoquinazolinehydroxamic acid compounds and applications thereof as antitumor medicaments. The compounds have good histone deacetylase inhibiting activity, have good inhibiting effects on multiple tumor cells of a human body, have weak inhibiting effects on normal cells and small toxicity, and are suitable for being developed as antitumor medicaments. The compounds also have good treatment effects on diseases caused by abnormal genetic expression. The 4-aminoquinazolinehydroxamic acid compounds are compounds or pharmaceutically acceptable salts shown as formula V.

The invention relates to a 3D folding paper base microfluid fluorescence detection device for simultaneously detecting multiple tumor markers, belonging to the technical field of biological detection. The detection device can be used for simultaneously detecting multiple tumor markers. A method of the invention comprises the following steps: (1) producing a paper base microfluid chip; (2) preprocessing the paper base microfluid chip; (3) carrying out immunoreaction program to simultaneously detect a three-dimensional paper base; and (4) carrying out fluorescence analysis and detection. A paper base microfluid analysis platform and a fluorescence detection manner are combined together and are used for simultaneously detecting multiple tumor markers. The detection device is simple, cheap, portable, disposable and easy to use, and a beneficial platform is provided for developing countries, resource-limited regions and remote regions.

The invention discloses a white light-emitting up-conversion nanoparticle and a test paper strip based on the white light-emitting up-converting nanoparticle and capable of simultaneously detecting multi-component tumor markers. According to the present invention, the nanoparticle has a three-layer core-shell structure, and emits white light under the excitation of near infrared light; and after the antibodies of multi-component tumor markers to be detected are labeled with the nanoparticle, the obtained conjugates are immobilized on the labeling complex binding zone of the test paper strip, such that the multiple tumor markers can be simultaneously detected; and with the nanoparticle and the test paper strip, the sensitive detection on the multiple tumor markers by the single up-conversion nanoparticle is achieved, and the advantages of simple operation, good specificity, high qualitative and quantitative real-time detection and high sensitivity are provided.

The invention belongs to the field of in-vitro diagnosis and particularly relates to a quantum dot immunochromatographic strip for synchronously quantifying multiple tumor markers and a method of the quantum dot immunochromatographic strip. The quantum dot immunochromatographic strip is characterized in that a marking pad (3) is coated with a mixture of various tumor marker antibodies marked corresponding to quantum dots with different wavelengths, a belt T (4) of an analyzing membrane (7) is coated with a mixture of various tumor marker antibodies, and a belt C (5) of the analyzing membrane (7) is coated with secondary antibodies; a standard curve of each tested object is stored in an electronic tag manner and the electronic tags are mounted on the immunochromatographic strip. According to the quantum dot immunochromatographic strip, a detection instrument having a signal detection function is utilized for reading standard curve data stored in the electronic tags and synchronously and quantitatively detecting concentrations of the various tumor markers in a to-be-detected sample by combining the corresponding fluorescence intensity of the to-be-detected sample measured by the detection instrument.

The invention belongs to the field of pharmaceutical chemistry, and particularly discloses 7-fluoro-substituted Isaindigotone derivatives. The structural formula of the derivatives is disclosed as Formula (I) or Formula (II), wherein X is C or N; R1 is hydrogen, amino, substituted amino, orpenta- or hexa-heterocyclic radical; R2 is hydrogen, hydroxy, amino, C1-C8 alkyl, or C1-C8 alkoxy or halo; R3, R5 and R6 are defined the same as R2; and R4 is hydrogen, hydroxy, amino, phenyl, C1-C8 cycloalkyl, C1-C8 alkyl, C1-C8 alkoxy, C1-C6 alkyl acyloxy, C1-C6 alkynyl, halo, or penta- or hexa-heterocyclic radical. The fluoro-substituted Isaindigotone derivatives can bebondedwith NM23-H2 protein and block the interaction between the NM23-H2 protein and c-myc G-quadruplex DNA, thereby causing the reduction of protooncogene c-myc transcription and translation, and inhibiting the proliferation of multiple tumor cell strains. The fluoro-substituted Isaindigotone derivatives have wide antitumor effects. The fluoro-substituted Isaindigotone derivatives are a novel NM23-H2-protein-targeted transcription regulation blocker, and have wide application space in preparing anticancer drugs.

The invention discloses a detection chip for a tumor driving gene and application thereof. The detection chip for the tumor driving gene comprises an ALK (anaplastic lymphoma kinase) fusion detection agent, an EGFR (epidermal growth factor receptor) fusion detection agent, an RET (reticulocyte) fusion detection agent and an ROS1 (reactive oxygen species 1) fusion detection agent. Proofed by the results of clinical detect, after fusion probes corresponding to the ALK, the EGFR, the RET and the ROS1 are specifically designed, the detection chip has the advantages that the sensitivity is high, and the gene fusion between a particular site area of the ALK, the EGFR, the RET and the ROS1 and fusion segments is specifically detected. The invention further discloses a detection chip for detecting a second sequence group of the gene mutation and a third sequence group of the gene amplification; after detecting once, the gene fusion, gene mutation and gene amplification of the multiple tumor driving genes, such as the ALK, BRAF (aserine / theroninespecific kinases), DDR2 (discordin domain receptor 2), the EGFR, ERBB2 (receptor tyrosine kinase 2), FGFR1 (fibroblast growth factor receptor 1), KRAS (kirstenrat sarcoma viral oncogene), MET (methionine), NRAS, PIK3CA (phosphatidylino-sitol 3-kinases), the RET and the ROS1.

The invention discloses a sandwich type electrochemistry immunosensor which is prepared based on a sodamide group smectite and a nanometer multi-hole gold membrane, and belongs to the technical field of function materials and biological sense. The sandwich type electrochemistry immunosensor provided by the invention can carry out amido functionalization on the sodium group smectite, an antibody and enzyme are conveniently fixed, and meanwhile, the favorable biological activities of the antibody and the enzyme are kept; a favorable three-dimensional serial perforated structure and biocompatibility of the nanometer multi-hole gold membrane are utilized, and the favorable catalytical property of the antibody is directly fixed, and the sensitivity of the sensor is notably improved; and the sodium group smectite which is cheap and is easily available is utilized, and the cost of the sensor is greatly reduced. The sandwich type electrochemistry immunosensor provided by the invention can realize high sensitiveness and specificity and rapid exact detection of multiple tumor markers.

The invention relates to an antitumor polypeptide for targeted inhibition on an ERK signal channel and application of the antitumor polypeptide. According to the antitumor polypeptide, a micro-molecule polypeptide for targeted inhibition on the ERK signal channel is established; a series of biological experiment shows that the micro-molecule polypeptide is capable of effectively inhibiting drug resistance of a PI3K / AKT inhibitor or a paclitaxel medicine in the antitumor treatment process for multiple tumors such as prostate cancer, breast cancer, colon cancer and rectal cancer, and has a remarkable synergic antitumor function; as the ERK signal channel exists in multiple tumors and is one of important signal channels for promoting cell growth and tumor generation and development, the micro-molecule polypeptide can be used for treating the tumors, and preferably the micro-molecule polypeptide is used together with the PI3K / AKT inhibitor or the paclitaxel medicine.

The invention discloses a joint detection kit for sialic acid and hydroxyproline. The kit comprises a color developing agent and a reference solution. The color developing agent is made from diethyl phthalate, ethyl acetate, 2,4-dinitrophenylhydrazine, hydrogen peroxide, hydrochloric acid and water, wherein the concentrations of the components are as follows: 0.35 mol / L of diethyl phthalate, 0.46 mol / L of ethyl acetate, 0.07 mol / L of 2,4-dinitrophenylhydrazine, 0.006 mol / L of hydrogen peroxide, and 0.62 mol / L of hydrochloric acid (hydrogen chloride). The reference solution is made from sialic acid, L-hydroxyproline and water, wherein the concentrations of the components are as follows: 0.3 g / L of sialic acid, and 0.25 g / L of L-hydroxyproline. With a specific color-developing analysis technology, in the same detection system, through primary reaction, multiple tumor markers can be reacted to develop colors. As the developed colors are superposed, the sensitivity of detection is improved. The joint detection kit complies with the trend of joint detection for tumor markers, is convenient and simple to operate, and is easy to promote and apply since the kit is operated without valuable equipment.