2318 results about "Microfluidic chip" patented technology

The invention provides an agricultural environment monitoring method and system based on the Internet of Things. According to the method, multi-source data weights are dynamically distributed through a soil sensor array connected with Internet of Things nodes, and a global soil parameter set is generated; deploying a micro-electro-mechanical micro-fluidic chip in the coverage area to carry out soil solution selective permeation, converting the target ion concentration into an electric signal, and synchronously transmitting the electric signal and soil parameters; historical time series data are extracted, coherence is established through time correlation analysis, and undeployed area data are filled in combination with a spatial interpolation algorithm to construct a dynamic prediction model; according to the nutrient space change trend output by the model, crop growth requirements are matched to generate a fertilization amount adjustment instruction, and the fertilization amount adjustment instruction is issued to field fertilization equipment through the Internet of Things to execute dynamic regulation. Space-time precise sensing of soil nutrients and self-adaptive fertilization regulation and control are realized, and the utilization efficiency of agricultural resources and crop growth sustainability are improved.

The invention relates to the technical field of model analysis, in particular to an imaging flow cytometry cell detection method based on an improved model. The method comprises the following steps: introducing a cell sample to be detected into an imaging flow cytometry system integrated with a micro-fluidic chip for continuous image acquisition to generate an initial cell image sequence; an automatic digital focusing algorithm is applied to the initial cell image sequence, and a cell image frame set with the optimal focal plane is screened out; inputting the cell image frame set into a preset PA-YOLO improved model for multi-dimensional extraction and fusion, and generating a multi-scale cell characteristic spectrum; carrying out refined feature learning and cell target positioning and classification on the multi-scale cell feature spectrum, and outputting a cell detection result; and carrying out validity verification on the cell detection result, and carrying out comparative analysis in combination with an imaging flow cytometry system to generate a cell detection report. According to the method, the imaging quality and the detection accuracy of cell images with different depths can be remarkably improved.

The present invention belongs to the field of microbead preparation technology, and specifically relates to a microbead production process with adjustable internal phase based on microfluidic technology and its application. The microbead production process includes the following steps: (1) raw material transportation: the required materials are transported to the microfluidic chip separately through a pump under heat preservation conditions; (2) logistics shearing: the required materials are sheared in the microfluidic chip to obtain composite droplets; (3) cooling and molding: after the sheared droplets are cooled, they are cooled and solidified using a cooling medium to form microbead particles; (4) product collection: the microbead particles and the cooling medium are subjected to solid-liquid separation, filtered, and collected to obtain microbeads. The microbead production process of the present invention can quickly realize the continuous production of double-layer microbeads, realize the precise regulation of the inner layer of the double-layer microbeads, and quickly and accurately control the particle size of the microbeads; at the same time, it can quickly realize the production conversion between single-layer microbeads and double-layer microbeads.

The invention discloses a generative odor real-time synthesis method and system based on cross-modal potential space mapping, and belongs to the technical field of artificial intelligence and olfaction calculation. The method comprises the following steps: acquiring a multi-modal input stream of a current scene, and extracting an emotion semantic feature vector by using a deep neural network; mapping the semantic features into target odor chemical feature vectors by using nonlinear projection through a pre-constructed vision-smell joint embedding space; constructing a convex optimization model based on olfactory perception, and calculating a basic liquid optimal mixing proportionality coefficient matrix capable of fitting the target vector; the matrix is converted into a micro-fluidic driving signal, and the target smell is synthesized in situ in the micro-fluidic chip. The invention further discloses a self-adaptive cleaning logic and olfactory fatigue compensation mechanism based on scene mutation detection. The method solves the problems that in the prior art, label matching is dependent, new smell cannot be synthesized, and dynamic transition is lacked, and olfactory replicating and real-time generation of abstract semantic scenes are achieved.

The invention relates to an organ-like core-shell microsphere as well as a preparation method and application thereof. The preparation method comprises the steps that 1, raw materials are prepared, specifically, shell raw materials and inner core raw materials are prepared, the shell raw materials comprise a photoinitiator and methacrylated hyaluronic acid (HAMA), and the inner core raw materials comprise organoid precursor cell suspension and matrigel; 2) molding: extruding the dispersion phase of the shell raw material wrapping the core raw material to a continuous phase by using a micro-fluidic chip and a high-precision injection pump, cutting the dispersion phase into liquid drops by the continuous phase, and performing illumination curing on the liquid drops to form core-shell microspheres; and (3) incubating, namely culturing the core-shell microspheres in a culture medium, so that the organoid precursor cells in the inner core are developed into organoids. According to the preparation method, rapid forming and curing of the organ-like precursor cells carried by the matrigel are achieved, so that the time of the organ-like precursor cells staying in the oil phase is shortened, the cells can obtain oxygen and nutrient substances, and the cell activity is improved.

The invention discloses an ultrasonic phased array driven graphene oxide flow stripping and closed-loop regulation and control system and method, and the system comprises an ultrasonic phased array module, and a piezoelectric transducer array element in the ultrasonic phased array module controls sound beam focusing and energy distribution through a phase delay controller, and carries out the stripping of graphite oxide flowing in a flow channel; a micro-Raman spectrometer in the micro-fluidic-Raman detection module collects characteristic peak data of a graphene oxide sheet layer in a micro-fluidic chip in real time and sends the characteristic peak data to a signal analysis unit; and the feedback control module is used for receiving the layer number distribution data output by the signal analysis unit, dynamically adjusting ultrasonic parameters of the phase delay controller, and controlling the diverter valve to guide substandard products into a secondary treatment channel and finally return the substandard products to the flow channel for stripping again. The system and the method for stripping the graphene oxide based on coupling of the ultrasonic phased array and the micro-fluidic chip have the advantages of efficient stripping, accurate detection, closed-loop optimization and the like.

The invention belongs to the technical field of microfluidics, and particularly relates to a liquid separation-convergence integrated liquid drop micro-fluidic chip and a liquid drop preparation method.The liquid separation-convergence integrated liquid drop micro-fluidic chip is characterized in that a liquid drop generation unit is arranged at the communication position of a branch splitting channel and a convergence channel which is correspondingly communicated with the branch splitting channel; and under the action of continuous phase shearing force, the dispersed phase solution is sheared off by the continuous phase solution, so that liquid drops are formed. The droplet generation units are symmetrically arranged along the circumference of the channel, the distance from each unit to the center is equal, and a continuous phase solution and a dispersed phase solution encountered at each droplet generation unit are almost equivalent, so that the sizes of the generated droplets are consistent, the droplets with basically consistent sizes are obtained at a collection port, and high-flux preparation of microspheres with uniform sizes is realized.

The invention discloses a portable nucleic acid detection micro-fluidic chip, a nucleic acid detection device and a nucleic acid detection method, and belongs to the field of micro-fluidic chips, a detection module comprises a quantitative chamber, a micro-channel, a reaction chamber, a first connecting channel and a color developing module; the reaction chamber comprises an RPA reaction chamber in which an RPA amplification reagent is pre-embedded, a porous filter membrane and a CRISPR reaction chamber in which a CRISPR detection component is pre-embedded, and the RPA reaction chamber, the porous filter membrane and the CRISPR reaction chamber are sequentially positioned at the same position of the three adjacent chip layers; the aperture of the porous filter membrane is larger than the particle size of an RPA amplification product and smaller than the particle size of a core enzyme in a CRISPR detection component; two ends of the micro-channel are respectively communicated with the quantitative chamber and the RPA reaction chamber; two ends of the first connecting channel are respectively communicated with the CRISPR reaction chamber and the color developing module; the flow resistance of the first connecting channel is smaller than that of the micro-channel. According to the invention, the sequential reaction of RPA amplification and CRISPR cleavage can be completed without depending on artificial transfer of amplification products.

The invention discloses a closed-loop control digital microfluidic system and a method thereof, and relates to the technical field of microfluidics, and the method comprises the following steps: initializing a digital microfluidic chip electrode array, setting an electrode spacing, an electrode size and a voltage upper limit, collecting a no-load state signal, and generating initial reference data; liquid drops are formed at the preset position of the digital micro-fluidic chip, and according to the initial reference data, the initial position, the volume and the form information of the liquid drops are collected, and a liquid drop initial state data set is generated; inputting the initial state data set of the liquid drop into a pre-trained closed-loop prediction model, performing kinetic analysis and trajectory prediction calculation, generating an expected motion trajectory of the liquid drop, and comparing the expected motion trajectory with the real-time state data flow of the liquid drop to generate deviation feedback data of the liquid drop; and dynamically adjusting the initial driving voltage parameter according to the droplet deviation feedback data to generate a closed-loop driving voltage sequence. The liquid drop trajectory control precision is improved, and motion errors caused by environmental disturbance and electrode response differences are reduced.

The invention discloses a tear total IGE detection method and system based on multi-modal data fusion. The method comprises the following steps: acquiring ocular surface images, physiological parameters and environmental allergen data, and acquiring historical medical data from a hospital information system; preprocessing and feature extraction are performed, patient symptom description is analyzed in combination with a natural language processing technology, and related image block features are recognized; adjusting a space-time attention weight based on frequency domain analysis and transfer learning, inputting a lightweight Transform model, and predicting a tear total IgE concentration and an allergy risk score; whether a trace tear sampling program is started or not is judged, and the actually measured concentration of total tear IgE is detected through a disposable micro-fluidic chip; and integrating all information by using a Bayesian adaptive filtering algorithm to determine the total tear IgE level. By implementing the method provided by the invention, the sampling amount is extremely small, the detection time is short, the environmental interference can be dynamically corrected, and the absolute concentration value is output, so that the dual requirements of clinical precise diagnosis and treatment and home monitoring are met.

The invention discloses a micro-fluidic chip, extraction equipment and an exosome extraction method, which can be used for an enzymatic detection device. Wherein the micro-fluidic chip comprises a chip base body with a rotation center, the chip base body is provided with a sample introduction unit, a reagent unit, a filtering unit, a capturing unit, a waste liquid bin, a collecting unit and a switching structure, and the filtering unit is arranged on the downstream of the sample introduction unit in the centrifugal direction, communicates with the sample introduction unit and is used for filtering impurities in a sample; the capturing unit is arranged on the downstream of the filtering unit in the centrifugal direction and communicated with the filtering unit, the capturing unit is communicated with the reagent unit and located on the downstream of the reagent unit, and the capturing unit is used for capturing or releasing the extracted target object; the waste liquid bin and the collecting unit are arranged on the downstream of the capturing unit in the centrifugal direction, and the waste liquid bin communicates with the capturing unit through a communicating structure; the switching structure is arranged in the communication structure, the switching structure can selectively block the communication structure, and the switching structure can enable the capturing unit to be communicated with the collecting unit.

The invention discloses a batch processing technology of micro-fluidic chips, and relates to the technical field of chip processing. The method comprises the following steps: firstly, providing at least two layers of polymer coiled materials, namely a first coiled material for forming a micro-channel structure and a second coiled material for closing a micro-channel, the coiled materials are continuous coiled materials selected from polyethylene glycol terephthalate (PET), cycloolefin polymer (COP), polycarbonate (PC) or polymethyl methacrylate (PMMA), and the coiled materials are continuous coiled materials selected from polyethylene glycol terephthalate (PET), cycloolefin polymer (COP), polycarbonate (PC) or polymethyl methacrylate (PMMA); the thickness of the coiled material is 50-500 microns; secondly, carrying out continuous compression molding processing on the surface of the first coiled material through a roll-to-roll processing platform; through a roll-to-roll continuous process, micro-channel mold pressing, functional deposition, precise alignment bonding and online cutting are integrated, high-efficiency, high-consistency and high-integration-level batch manufacturing of the micro-fluidic chip is realized, and manual intervention and production cost are remarkably reduced.

The utility model relates to the technical field of micro-fluidic chips, in particular to a micro-fluidic chip which comprises a chip main body, a sample injection hole, a flow channel, a reaction hole and an air injection hole, the sample injection hole, the flow channel and the reaction hole are positioned on the chip main body, the sample injection hole, the flow channel and the reaction hole are sequentially connected, and the air injection hole is communicated with the flow channel. The flow channel comprises a sample injection flow channel and a driving flow channel, the sample injection hole, the sample injection flow channel, the flow blocking part, the driving flow channel and the reaction hole are sequentially connected, an inner cavity of the flow blocking part is provided with a flow blocking plug capable of preventing the sample injection flow channel from being communicated with the driving flow channel, the flow blocking plug is in sliding connection with the flow blocking part, and the gas injection hole is located in the driving flow channel. A sealing plug is slidably connected into the gas injection hole. According to the micro-fluidic chip disclosed by the utility model, when the sample liquid in the flow channel is driven to advance by extruding the gas, the gas can be prevented from flowing back in the flow channel, and the volume of the sample liquid entering the reaction area can be obtained through the volume of the gas injected into the flow channel, so that the quantitative control of the sample liquid is realized.

The invention discloses a micro-fluidic chip for basalt CO2 mineralization reaction and a preparation method, the micro-fluidic chip comprises a bottom glass plate, a middle epoxy resin layer and a top glass plate, the preparation process comprises the following steps: installing a metal injection / outpouring pipeline on the bottom glass plate drilled with corresponding bolt holes, and forming an accommodating cavity by using a sealing ring; preparing and screening basalt particles according to the particle size distribution of a target reservoir, and filling the accommodating cavity with the basalt particles; quantitatively analyzing a particle arrangement structure based on a digital image processing technology, ensuring that the geometric similarity between the particle arrangement structure and a target reservoir is greater than or equal to 90%, and if the particle arrangement structure does not reach the standard, refilling; after the verification is passed, covering a top glass plate and pressing by using a bolt; and finally, pouring epoxy resin and sealing to form the micro-fluidic chip. The chip can bear high temperature and high pressure, supports visual observation and quantitative analysis of CO2 mineralization reaction in a deep geological environment, has high-precision pore reproduction, long-period sealing performance and high preparation efficiency, and is suitable for interdisciplinary research in the fields of energy environment and geophysics.

The invention relates to a microfluidic nucleic acid detection device, and relates to the field of detection. Comprising a bottom plate, a chip inlet and outlet module erected on one side above the bottom plate, a chip table erected on the chip inlet and outlet module and used for bearing a micro-fluidic chip with a liquid bag, a displacement module erected above the chip table, and a crushing module erected on the displacement module and used for squeezing liquid in the liquid bag into the micro-fluidic chip, the liquid transferring module is erected on one side of the crushing module and is used for transferring liquid in the micro-fluidic chip to flow; the pneumatic module is erected above the front end of the bottom plate and is used for controlling a flow channel of the micro-fluidic chip to be opened and closed; the heating module is erected on the other side above the bottom plate and is used for locally heating the micro-fluidic chip and automatically detecting the temperature; and the fluorescent module is used for acquiring a fluorescent signal of the micro-fluidic chip. The micro-fluidic chip detection device has the advantages that different types of micro-fluidic chips can be rapidly detected, and an accurate result can be obtained.

The invention discloses a gene sequencing sample data matching method based on a micro-fluidic chip, relates to the technical field of gene sequencing sample matching, and aims to solve the problem that the matching rate is reduced due to inaccurate analysis of gene sequencing samples. According to the method, multi-strategy comparison and candidate set screening are adopted, multi-dimensional comparison of sequences, variation and functions is combined, the accuracy is improved, the adaptability to complex samples is enhanced through quantitative index and biological verification evaluation and dynamic optimization of comparison strategies, the matching reliability and practical value are remarkably improved, the clinical and scientific research diversified requirements are met, and the method is worthy of popularization and application. Sample types such as blood and cells are adapted through a differential lysis strategy, impurities such as proteins and salts are removed through stepped purification, and the nucleic acid concentration and the fragment state are unified in combination with standardized treatment, so that interference is reduced from the source.

The invention discloses a temperature control system and method of a micro-fluidic chip, and relates to the technical field of micro-fluidic temperature control, and the method comprises the following steps: in the manufacturing process of the micro-fluidic chip, embedding topological insulator nanowires into a substrate in a directional arrangement mode to form a pre-locking array, and meanwhile, generating an anisotropic heat conduction network under the continuous action of a sound-magnetic field, a topological vortex invariant is calculated, and a heat conduction weight coefficient is generated; arranging a fluorescent layer on the surface of a micro-reaction cavity of the anisotropic heat conduction network, collecting a fluorescence lifetime attenuation curve in real time, and calculating a transient temperature value and a temperature change rate according to a heat conduction constraint variation model and a heat conduction weight system; nonlinear feed-forward compensation power is calculated according to the transient temperature value and the temperature change rate, feedback compensation power is calculated in combination with a deviation field of the target temperature and the real-time temperature, and a compensation power instruction is generated; according to the method, the thermal pilot frequency domain filtering function is constructed through the compensation power instruction, so that the uniformity and response speed of thermal response are improved.

The invention provides a micro-fluidic chip capable of culturing oral squamous cell carcinoma organoid and a preparation method thereof, and relates to the technical field of micro-fluidic chips, the chip comprises a four-layer structure: the first layer is a closed layer, the second layer is a culture solution / drug pool layer (containing a concentration gradient injection channel and a slow flow channel), the third layer is a matrix culture pool layer, and the fourth layer is a matrix culture pool layer. The fourth layer is a matrigel inflow layer (containing an S-shaped flow channel); the second layer and the third layer are integrally formed, and all the layers are connected through oxygen plasma welding; the problems that in the prior art, matrigel is prone to shifting, drug gradient regulation and control is poor, operation is tedious, and adaptability is insufficient are solved, the matrigel and organoids can be stably fixed, the drug concentration gradient can be precisely regulated and controlled, the nutrition supply efficiency is enhanced, the structural integration level and operation convenience are improved, and the application prospect is wide. The method is suitable for oral squamous cell carcinoma organoid culture, drug screening and tumor mechanism research.

The invention discloses a detection kit and method for detecting allergic inflammatory factors based on a centrifugal micro-fluidic chip, and relates to the field of kits, the detection kit comprises the centrifugal micro-fluidic chip and a preset reagent; the centrifugal micro-fluidic chip comprises a chip base body, a sample adding cavity, a liquid level sensor, a liquid level sensor, a liquid level sensor, a liquid level sensor and a liquid level sensor, the nucleic acid extraction and purification cavity comprises a plurality of amplification cavities; a reagent storage chamber; a waste liquid chamber; and a capillary valve. The functions of nucleic acid extraction, variable temperature amplification and multi-target synchronous detection are integrated through the centrifugal micro-fluidic chip, full-closed operation is achieved by means of centrifugal force or other driving force such as air pressure and electroosmotic flow, and the pollution risk caused by manual step-by-step operation is thoroughly eliminated; a multi-wavelength distinguishable fluorescent probe and a grouping preset primer combination are adopted to synchronously complete parallel detection of the allergic inflammatory factor mRNA and the drug accompanying diagnosis target, cross interference is avoided, and large instruments and equipment do not need to be relied on.

The invention provides an oil aliasing particle detection device based on a multi-frequency eddy current effect, which adopts a multi-mode electromagnetic sensing architecture, integrates three groups of orthogonally arranged solenoid coil detection units in a single detection channel, and respectively loads low-frequency (10-100kHz), intermediate-frequency (100kHz-1MHz) and high-frequency (1-10MHz) excitation signals with different characteristics, so as to detect the aliasing particles in the oil. Based on frequency domain response characteristic differences presented by different metal particles in high, medium and low frequency eddy current fields, a particle material-size-space distribution frequency domain characteristic matrix is constructed through multi-physics field coupling modeling, and synchronous identification and orthogonal decoupling of ferromagnetic particles and non-ferromagnetic particles are realized. According to the invention, the eddy current effect difference characteristics of metal / non-metal particles under excitation of different frequencies are utilized, and a signal decoupling algorithm is combined to realize accurate distinguishing of aliasing particles, so that the reliability of high-flux oil detection is remarkably improved; and a key technical support is provided for engineering application of a microfluid chip technology in the fields of engine wear monitoring, hydraulic system fault early warning and the like.

The invention provides a micro-fluidic chip, a model for realizing accurate positioning of an organoid and sequential interaction of a tumor microenvironment, and a construction method of the model. The micro-fluidic chip comprises a chip main body and a chip bottom plate, a blood vessel channel, a central channel, a neural immune cell channel and a culture medium channel are sequentially arranged on the lower surface of the chip main body; the chip bottom plate is arranged on the lower surface of the chip main body and seals each channel; one side of the blood vessel channel is communicated with one side of the central channel, the other side of the central channel is communicated with one side of the neural immune cell channel, and the other side of the neural immune cell channel is communicated with one side of the culture medium channel; the upper surface of the chip main body is provided with an open hole, and the open hole penetrates through the chip main body and is communicated with the middle position of the central channel. According to the model manufactured by the micro-fluidic chip, accurate positioning of tumor organs can be realized through combination of an automatic positioning device and physical constraint, and multi-dimensional cell interaction is supported by dynamic environment regulation and control.

The invention belongs to the technical field of biological detection, and particularly relates to a rapid detection method and a micro-fluidic chip for rapid detection. The existing detection method and instrument have the problems of long detection time, incapability of detecting unknown pathogenic bacteria and the like, and cannot meet the timeliness requirement of clinical application. According to the scheme, rapid detection of the drug resistance of bacteria is realized by detecting the growth state of high-throughput single bacteria, and the method comprises the following steps: culturing the bacteria by using the micro-fluidic chip designed in the invention, capturing the single bacteria through a single bacteria capturing channel, and continuously growing in the channel; and obtaining a morphological change matrix of single bacteria evolved along with time in each bacteria capture channel, carrying out statistical analysis to obtain a bacteria phenotype index, and further obtaining the drug tolerance condition of the bacteria on the basis of index numerical value change. According to the method, the function of rapidly detecting the drug resistance of various common clinical pathogenic bacteria within 2 hours can be realized.

The invention belongs to the technical field of ultra-precision machining, and discloses a micro-fluidic chip based on the electroosmosis effect and a stress regulation and control method thereof.The micro-fluidic chip is internally provided with a plurality of solution channels, each solution channel is of a snakelike reciprocating structure, one end of each solution channel is provided with a channel inlet, and the other end of each solution channel is provided with a channel outlet; a plurality of inflection points arranged at intervals are arranged on one side, facing the bottom of the micro-fluidic chip, of the solution channel, the inflection points are close to the bottom of the micro-fluidic chip and are distributed on the micro-fluidic chip in the horizontal direction, and an electrode is further arranged on the solution channel. The electroosmosis effect and the microfluidic technology are combined, high-precision dynamic stress regulation and control are achieved through gradient flow channel design, a composite driving liquid formula and a hysteresis compensation algorithm, and the problems that pressure control response is delayed and precision is insufficient in a traditional polishing technology are solved; and a new technical approach is provided for ultra-precision machining of the large-aperture optical element for the inertial confinement nuclear fusion device.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to an integrated micro-fluidic card box. The device comprises a first solution storage part, an adsorption part, a second solution storage part and a reaction part, the bottom surface of the first solution storage part is rotationally connected with the top surface of the adsorption part, the bottom surface of the adsorption part is rotationally connected with the top surface of the second solution storage part, and the bottom surface of the second solution storage part abuts against the top surface of the reaction part; a second channel penetrates through the first solution storage part, a second runner is arranged on the adsorption part, and a second through hole penetrates through one end of the adsorption part; during single-sample nucleic acid detection, lysate feeding, cleaning liquid feeding, eluent feeding and sample loading share an exhaust channel formed by the second flow channel and the second channel, and the flow process of the solution can be controlled only by one driving pump; the driving pump and the external pipeline only discharge gas but not liquid, so that the solution is prevented from being remained when passing through the pump and the pipeline, and pollution is avoided; a plurality of micro-fluidic chips are communicated with the driving pump and the controller, so that a plurality of samples can be detected at the same time, and the detection efficiency is improved.

The invention relates to the field of cell analysis, and provides a multi-mode digital micro-fluidic system and method for cell sorting analysis, the system comprises an active digital micro-fluidic platform, the active digital micro-fluidic platform comprises a micro-fluidic chip, an upper computer control system and an FPGA hardware circuit, an upper computer generates a liquid drop path planning instruction, the FPGA hardware circuit converts the liquid drop path planning instruction into an electrode driving signal, and the electrode driving signal is transmitted to the micro-fluidic chip; the nanoliter-grade liquid drops are driven to change between the electrodes; the optical tweezers platform comprises a laser device, an optical microscope and a CMOS camera, laser beams are generated by the laser device to form a three-dimensional optical potential well, the laser beams are transmitted into the micro-fluidic chip through the optical microscope to realize non-contact control on cells, and the CMOS camera records images and transmits the images back to the upper computer; the objective table clamp is used for realizing cooperative control of the optical tweezers and the active digital microfluidics; and the image recognition system is used for recognizing morphological characteristics of cells in the liquid drops based on a convolutional neural network and screening target cells. According to the invention, integration of high-throughput nondestructive cell sorting and in-situ multi-modal analysis is realized.

The invention provides a device and a method for detecting antibiotics in aquaculture water, and belongs to the technical field of environmental pollutant detection. According to the device disclosed by the invention, a Fe3O4 (at) GO magnetic nano adsorption material is packaged in a micro-fluidic chip reaction chamber, and a magnetic sticker is innovatively adopted to realize immobilization and replaceable design of an adsorbent; the integrated electromagnetic oscillation module drives the adsorbent to vibrate in the microchannel, and the mass transfer capture efficiency of antibiotics such as sulfonamides, quinolones and tetracyclines is remarkably improved. According to the method, an adsorption-elution-detection integrated process is initiated for the first time, the pretreatment time is shortened to be within 10 minutes from the hour level, synchronous detection of 0.01-5 [mu] g / L ultra-trace multi-component antibiotics in 5-10 [mu] L of a water sample is achieved, compared with a traditional method, the detection precision is remarkably improved, the matrix interference resistance is high, and the method is suitable for large-scale popularization and application. The method is suitable for real-time online monitoring of multiple antibiotic residues in the aquaculture environment.

The invention provides a silk fibroin-based physical microgel material and a preparation method thereof, and belongs to the field of biomedical materials. According to the preparation method, an organic solvent is added into a silk fibroin solution, micron-sized gel particles with different sizes and different silk fibroin contents can be obtained by regulating and controlling the water-oil flow rate ratio and the silk fibroin concentration through the microfluidic technology, and the organic solvent and polysaccharide can be added into the silk fibroin solution at the same time; and the polysaccharide-silk fibroin mixed microgel is obtained through a microfluidic technology. The method is simple to operate and cost-saving, not only accelerates the sol-gel conversion speed of the silk fibroin, reduces the viscosity of the solution to enable the solution to pass through the micro-fluidic chip more easily, but also can promote the stability of the mixed solution of the silk fibroin and the polysaccharide, improves the mechanical property of the silk fibroin microgel and improves the stability of the micro-fluidic chip. The obtained silk fibroin-based microgel is uniform in size, regular in shape and high in porosity.

The utility model discloses a particle pairing device and a pairing system, the pairing device is used for controlling pairing of a first particle and a second particle in a micro-fluidic chip, and the particle pairing device comprises a detection module and a valve control module electrically connected to the detection module, the detection module is used for identifying first particles when a first particle solution flows through a first particle flow channel of the micro-fluidic chip, is also used for identifying second particles when a second particle solution flows through a second particle flow channel of the micro-fluidic chip, and is electrically connected to the valve control module; the valve control module is used for blocking the first particle flow channel when the detection module recognizes the first particles and blocking the second particle flow channel when the detection module recognizes the second particles. Therefore, pairing of a single first particle and a single second particle can be realized, for example, when the first particle and the second particle are respectively cells and microspheres, the accuracy and efficiency of pairing of the cells and the microspheres can be improved, and then the efficiency of single cell sequencing is improved.

The invention discloses a dual-mode micro-droplet generation micro-fluidic chip and application thereof in generation of dual-emulsion droplets, and relates to the technical field of micro-fluidic chips. The method comprises the following steps: respectively injecting two different internal phase solutions from the left ends of respective iron tubes, guiding the internal phase solutions to a wedge-shaped tube orifice of a theta-shaped glass capillary tube through respective thin tubes, injecting an intermediate phase solution into a square glass tube through an intermediate phase inlet by using a No.1 needle, and injecting an external phase solution into the square glass tube through an external phase inlet by using a No.2 needle; the two internal phase solutions, the intermediate phase solution and the external phase solution converge at an inlet of the circular glass capillary tube, and after entering the circular glass capillary tube, water-in-oil-in-water double-emulsion droplets are obtained. The invention can obtain the dual-mode micro-droplet generation micro-fluidic chip and the application of the dual-mode micro-droplet generation micro-fluidic chip in generation of dual-emulsion droplets.

The invention relates to the field of microbiological detection, in particular to a method for detecting vibrio parahaemolyticus by combining RPA-CRISPR / Cas12a with a microfluidic biosensor. According to the method, the preloaded freeze-drying reagent is combined with centrifugally driven liquid operation, so that rapid and closed system detection is realized, and the pollution risk is effectively reduced. According to the invention, quantitative detection of vibrio parahaemolyticus is realized in a centrifugal micro-fluidic chip by systematically optimizing an RPA amplification primer and crRNA. The detection method shows high specificity and high sensitivity to non-target vibrio and other food-borne bacteria, and the lower detection limit is 6.08 copies / mu L. In a word, the invention provides a rapid, portable and sensitive platform, can realize quantitative detection in a wide linear range, provides a practical tool for real-time monitoring of pathogens, and has application prospects in monitoring of aquaculture biosafety and marine product safety.