4528 results about "Microfluidic chip" patented technology

The invention provides an agricultural environment monitoring method and system based on the Internet of Things. According to the method, multi-source data weights are dynamically distributed through a soil sensor array connected with Internet of Things nodes, and a global soil parameter set is generated; deploying a micro-electro-mechanical micro-fluidic chip in the coverage area to carry out soil solution selective permeation, converting the target ion concentration into an electric signal, and synchronously transmitting the electric signal and soil parameters; historical time series data are extracted, coherence is established through time correlation analysis, and undeployed area data are filled in combination with a spatial interpolation algorithm to construct a dynamic prediction model; according to the nutrient space change trend output by the model, crop growth requirements are matched to generate a fertilization amount adjustment instruction, and the fertilization amount adjustment instruction is issued to field fertilization equipment through the Internet of Things to execute dynamic regulation. Space-time precise sensing of soil nutrients and self-adaptive fertilization regulation and control are realized, and the utilization efficiency of agricultural resources and crop growth sustainability are improved.

The invention relates to the technical field of fluid control, and discloses a fluid control intelligent adjusting method and system for a micro-fluidic chip, and the method comprises the steps: carrying out the data collection of a self-adaptive fluid controller, a micro-fluidic channel pressure sensor array, a temperature sensor array and a flow sensor array based on the micro-fluidic chip; obtaining real-time fluid parameter monitoring data; performing feature extraction to obtain a multi-dimensional fluid feature matrix; establishing a three-dimensional fluid model of the microfluidic channel, and solving to obtain flow field distribution data; inputting the flow field distribution data into a Kriging agent model, establishing a mapping model of channel width, depth, length and fluid control performance, and calculating to obtain an optimal geometric parameter combination; and performing multi-level calculation on the fluid pressure uniformity, the temperature distribution uniformity and the flow stability to obtain a hierarchical fluid parameter control strategy. According to the invention, the problem of multi-physics field coupling is effectively solved, the calculation complexity of the control system is reduced, and the execution efficiency of the control strategy is improved.

The present invention relates to the technical field of microfluidics, and discloses a microfluidic chip, which comprises a mixing detection module, a control valve and a power module, and the mixing detection module comprises a plurality of storage cavities, a first channel, a second channel, a third channel, a common channel, a mixing cavity and a detection cavity. According to the invention, the storage cavity for providing the reagent, the channel for fluid such as the reagent and the sample to flow, the mixing cavity for mixing and reacting the fluid such as the reagent and the sample, and the detection cavity for analyzing and detecting the sample are integrated on the mixing detection module; a control valve and a power module which are used for realizing directional flow and mixed reaction of fluid in each channel are arranged as parts which can be removed relative to the mixed detection module, so that the integration degree of the micro-fluidic chip can be improved, and the volume of the micro-fluidic chip can be reduced; in addition, the installation and replacement of the mixed detection module serving as a consumable are facilitated, and the flexibility and convenience of the micro-fluidic chip in use are effectively improved.

The invention relates to the technical field of drug concentration detection, and relates to a clinical pharmaceutical blood concentration detection method, which comprises the following steps: capturing drug molecules in a blood sample based on a micro-fluidic chip, enabling a sensor to detect the drug molecules and combine with the surface of the drug molecules, and collecting high-sensitivity blood reaction data. According to the method, main variables in data are identified and utilized by adopting a principal component analysis method, so that the complexity of spectral data is effectively reduced, meanwhile, the most critical information for calculating the drug concentration is reserved, the data processing flow is optimized, the analysis speed and efficiency are improved, and the prediction of the drug concentration is more accurate; according to the method, more reliable data support is provided for clinic, the data is smoothed through the moving average algorithm, random fluctuation caused by sampling errors or external variables is reduced, the overall quality and credibility of the data are improved, errors can be obviously reduced, and therefore doctors are supported to conduct more accurate medicine dosage adjustment and curative effect evaluation.

The invention relates to the field of chip packaging testing, in particular to an automatic packaging testing method and system for a micro-fluidic chip. The method comprises the following steps: acquiring a real-time monitoring image of a micro-fluidic chip to be packaged in a packaging box; performing chip position alignment calibration processing on the real-time monitoring image of the micro-fluidic chip, and collecting a calibrated chip monitoring image; performing chip internal component visual inspection and multi-channel flow topological correlation fitting on the calibrated chip monitoring image, and constructing a chip packaging micro-channel topological structure model; and obtaining a packaging demand log, performing automatic packaging and filling execution on the chip packaging micro-channel topological structure model, and collecting environment monitoring parameters of the microfluidic packaging box. The packaging technology is continuously optimized through real-time feedback, the packaging test parameters are automatically adjusted, and the stability and precision of the packaging technology are gradually improved.

The invention discloses an intelligent detection method for water pollutants, which comprises the following steps of: transmitting a signal of a specific frequency band to a water body through an electromagnetic wave transmitting module, acquiring reflected and refracted signals by using a multi-channel receiver, extracting the characteristics of dielectric constant, refractive index and the like of the water body, and analyzing the types and the concentration range of the pollutants by adopting a pattern recognition algorithm; the method comprises the following steps: selecting a micro-fluidic chip and an electrochemical unit, selecting a corresponding biosensor for targeted detection, processing detection data through the micro-fluidic chip and the electrochemical unit, predicting the concentration, spatial distribution and trend of pollutants through a data analysis module, setting a dynamic self-adaptive threshold by the system, realizing pollution early warning, and uploading data to a cloud platform through a remote communication module. And remote monitoring is supported. The method can be widely applied to the fields of water quality monitoring, environmental protection and pollution control, and the detection precision, the real-time performance and the intelligent level are improved.

The invention discloses a micro-fluidic chip device for cell fusion and in-situ sorting. The micro-fluidic chip device comprises a PDMS (Polydimethylsiloxane) micro-fluidic chip, a PCB (Printed Circuit Board) and a patterned gold electrode micro-array layer which are sequentially arranged from top to bottom, the PDMS micro-fluidic chip is provided with a sample inlet, a sample outlet and a collection cavity, and the sample inlet, the sample outlet and the collection cavity are communicated through the experiment cavity; a plurality of independent channels and a capturing cavity connected with the independent channels are arranged in the experiment cavity, and a double-cavity capturing structure is arranged at an inlet of the capturing cavity; and an independent microelectrode matched with the double-cavity capture structure is arranged on the patterned gold electrode microarray. The independent microelectrodes on the patterned gold electrode microarray layer are matched with the double-cavity capture structure, so that cell capture, cell electrofusion and cell in-situ release can be realized in the same chip, and cells in the double-cavity capture structure can be controlled more accurately.

The invention discloses a CTC cell detection method based on a micro-fluidic chip, and belongs to the technical field of cell treatment. In order to solve the problems of high false positive and false negative rates, time consumption and low efficiency caused by difficulty in accurately distinguishing CTC from other cell types, the invention can efficiently and specifically capture CTC cells, reduce the possibility of false positive and false negative and improve the detection accuracy by constructing a specific capture chamber and filling specific antibodies and immunomagnetic beads. The multi-channel design allows a plurality of samples to be processed at the same time, the detection efficiency is improved, the micro-fluidic chip technology integrates a plurality of steps on one platform, automation and standardization of operation are achieved, personal errors are reduced, the stability and repeatability of detection are improved, the uniformity and stability of the detection process are ensured, and the detection efficiency is improved. The fluorescence imaging technology clearly displays CTC and obtains key parameters such as quantity, form and fluorescence intensity of CTC, thereby providing powerful support for cancer diagnosis and research.

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

Provided are a double-cavity micro-fluidic chip having a reaction cavity and a test cavity and used for nucleic acid test, and a test method thereof. A fluid channel is provided between the reaction cavity and the test cavity, and only one exhaust channel is provided. The combination of the fluid channel and the exhaust channel can automatically provide resistance for a sample entering the test cavity from the reaction cavity, thereby achieving a controllable transfer of the sample between the reaction cavity and the test cavity. A gravity micro-fluidic chip and a centrifugal micro-fluidic chip are designed. The gravity micro-fluidic chip has a multi-turning circuitous fluid channel of which the diameter is smaller than that of a sample injection channel, such that under the action of gravity, the sample can flow into a reaction cavity from a sample adding region but cannot flow into the fluid channel, and then under pressurization by a simple pressurization device, the sample can enter a test cavity from the reaction cavity. The centrifugal micro-fluidic chip provides an exhaust channel branched from a fluid channel, and makes the exhaust direction opposite to a rotary centrifugal direction and the position of an exhaust opening higher than that of the fluid channel; therefore, by combining the principle of internal and external balance of the atmospheric pressure and the centrifugal driving performance, the centrifugal micro-fluidic chip effectively controls the flowing of a gas and a liquid in reaction cavities and test cavities, so as to effectively control the amount of the sample entering each cavity, so that multiple nucleic acid tests can be completed simply by means of one or two times of centrifugation. In addition, for a chip which only has a reaction cavity, an exhaust channel is connected to a liquid storage cavity, and a gravity-driving chip and an asymmetric pressure-driving chip are designed, thereby effectively avoiding aerosol pollution.

The invention provides a microfluidic chip, system and method for osteochondral organs, and relates to the technical field of organoid microfluidic chips, the chip comprises a bone microvascular endothelial cell channel, a bone compartment, a cartilage compartment and a synovial fluid channel which are arranged in parallel and in sequence, and permeation areas between the channel and the compartments and between the compartments. According to the scheme, the complex structure and function of a human osteochondral system are effectively simulated, the physiological and pathological processes of human bone joints can be simulated more truly in an in-vitro environment, and a method for establishing a highly-simulated femoral head necrosis model and osteoarthritis model based on the chip is provided. The problems that a traditional model is insufficient in physiological environment simulation, lack of control over dynamic culture conditions, tedious and time-consuming in the construction process, difficult to support high-throughput experiments and the like are solved.

The invention relates to the field of physiological status detection, and provides a wearable microfluidic fluorescence sensor based on a Janus membrane for sweat analysis. The sensor comprises a Janus membrane-based micro-fluidic chip and a fluorescent sensing layer which are stacked, the Janus membrane-based micro-fluidic chip comprises a hydrophobic nanofiber membrane, a bottom polydimethylsiloxane layer, a hydrophilic nanofiber membrane and a top polydimethylsiloxane layer which are stacked, and the fluorescent sensing layer comprises three paper chips, the simultaneous fluorescence detection of chloride ions, glucose and pH of sweat is realized. The Janus membrane with the directional water conveying capacity is used as a substrate for preparing a micro-fluidic chip. In one aspect, sweat may be automatically delivered to an analysis zone along a designed path. On the other hand, sweat is transferred from a hydrophobic membrane close to the skin to a hydrophilic membrane, sweat accumulation is effectively avoided, and a comfortable skin microenvironment is promoted. A Janus membrane-based micro-fluidic chip is combined with fluorescence sensing, a micro-fluidic fluorescence sensor based on a Janus membrane is constructed, and simultaneous high-stability and high-accuracy analysis of multiple targets in human sweat is realized.

The invention discloses a dynamic optimization system and method for a compound essential oil ratio, and aims to solve the problems that the traditional technology depends on artificial experience, the optimization efficiency is low and the quality tracing is insufficient. According to the method, raw material molecular fingerprint data are collected in real time through a high-precision spectrum sensor array and a micro-fluidic chip, a production environment field domain is constructed in combination with a computational fluid mechanics model, a formula optimization problem is encoded into an Ising model by using a quantum annealing algorithm, and global search is realized through a quantum tunneling effect. The system integrates digital twin bodies, covers molecular dynamics simulation, phase equilibrium prediction and olfactory receptor activation probability simulation, and accurately maps a physical system state; a multi-modal data fusion network and a reinforcement learning framework are adopted to dynamically adjust formula parameters, and an NSGA-III algorithm is combined to optimize efficacy collaboration degree, cost and stability indexes. And the execution control module realizes nanoscale flow regulation through the piezoelectric micro-injection valve array, and realizes non-tampering evidence storage of formula parameters and quality data based on a block chain technology. Dynamic closed-loop optimization of the compound essential oil ratio is achieved, the production efficiency, the product consistency and the supply chain transparency are remarkably improved, and the method is suitable for the field of intelligent manufacturing of high-end essential oil.

The invention relates to the technical field of model analysis, in particular to an imaging flow cytometry cell detection method based on an improved model. The method comprises the following steps: introducing a cell sample to be detected into an imaging flow cytometry system integrated with a micro-fluidic chip for continuous image acquisition to generate an initial cell image sequence; an automatic digital focusing algorithm is applied to the initial cell image sequence, and a cell image frame set with the optimal focal plane is screened out; inputting the cell image frame set into a preset PA-YOLO improved model for multi-dimensional extraction and fusion, and generating a multi-scale cell characteristic spectrum; carrying out refined feature learning and cell target positioning and classification on the multi-scale cell feature spectrum, and outputting a cell detection result; and carrying out validity verification on the cell detection result, and carrying out comparative analysis in combination with an imaging flow cytometry system to generate a cell detection report. According to the method, the imaging quality and the detection accuracy of cell images with different depths can be remarkably improved.

The invention discloses a method for preparing lubricating oil microspheres based on a microfluidic technology. The method comprises the following steps: preparing an external phase solution, an intermediate phase solution, an internal phase solution and a collection solution; firstly, the internal phase solution is introduced into the micro-fluidic chip, and after the flow of the internal phase solution is stable, the intermediate phase solution is introduced into the micro-fluidic chip to be sheared with the internal phase solution to form nuclear layer liquid drops. After the core layer liquid drops are generated stably, the external phase solution is introduced into the micro-fluidic chip to be sheared with the core layer liquid drops, so that the external phase solution wraps the outer layer of the core layer to form a shell layer with a core-shell structure, and then the double liquid drops are dropped into a collecting solution containing glutaraldehyde to be crosslinked and cured to form the chitosan lubricating oil microspheres. The chitosan lubricating oil microsphere has the following beneficial effects that the lubricating oil is wrapped in the microsphere through a microfluidic technology, so that the chitosan lubricating oil microsphere is of a core-shell structure, and when the lubricating oil is needed, the lubricating oil in the microsphere is released to improve the friction performance of parts.

The invention relates to the field of microfluid chemical synthesis, and discloses a method for preparing gold nanoparticles based on two-phase flow microfluid control. The method comprises the following steps: 1) converging a gold precursor water phase, a reducing agent water phase and an organic phase in a micro-fluidic chip at 0-25 DEG C to form water-in-oil liquid drops; (2) conveying the fluid to a mixing area channel of a micro-fluidic chip, and carrying out instantaneous nucleation reaction to form a gold nanoparticle prototype; 3) conveying the fluid into a capillary reactor, and further nucleating, growing and curing the gold nanoparticle prototype; and (4) the fluid is subjected to phase splitting, and a gold nanoparticle solution is obtained. A three-in-one micro-fluidic chip is adopted, process parameters are optimized, and a gold precursor and a reducing agent are wrapped in an organic phase before reaction to form water-in-oil liquid drops, so that on one hand, the situation of adsorption and scaling of the inner wall of a channel can be avoided, and on the other hand, the heat and mass transfer efficiency of a material can be improved through the generated liquid drops; and the uniformity of a reaction nucleation environment is ensured.

Embodiments of the invention include additional methods and compositions for fabricating organoids. A unique approach has been developed for the rapid aggregation of defined number of cells using a micropillar-based template to limit the size of organoids, which are then more commonly referred to as spheroids. This procedure can be utilized to create in vitro cardiac tissue models (e.g., cardiac organoids) simply, rapidly, and inexpensively. In certain aspects an alginate-gelatin based hydrogel premixed with cardiomyocytes is used in combination with a patterned microfluidic chip / cell culture chamber having an array of micropillars to fabricate 3D cardiac organoids.

The invention discloses a control system and method for multi-channel treatment of a biological sample. A capture antibody layer of an optical fiber SPR sensor and a real-time detection biological solution can be prepared; comprising a microfluidic module, a sensing module and a computer, the sensing module is used for monitoring resonance wavelength information of a biological solution flowing through a sensing area of the optical fiber SPR sensor in real time; the micro-fluidic module is used for enabling the solution in the corresponding liquid storage tank to flow through the outer surface of the optical fiber SPR sensor in the micro-fluidic chip through the micro-fluidic channel under the set pressure; and the computer is used for detecting the corresponding content of the biological solution in the current liquid storage tank by measuring the resonant wavelength and comparing the resonant wavelength with pre-stored data based on the received electric signal of the spectrum. According to the control system, a micro-fluidic chip is used as a container for accommodating a sample to be detected or prepared, and a biological solution slowly flows into the micro-fluidic chip through a micro-fluid channel so as to realize preparation of a capture antibody layer or real-time detection of the biological solution.

The detection method comprises the following steps: adding an oxygen-containing solution into a blood sample, adding the oxygen-containing solution into a micro-fluidic chip, irradiating the blood sample through light sources with different wavelengths, and detecting data of absorbed light to obtain concentration information and establish a model; the contribution of hemoglobin in the measurement concentration of the blood sample is obtained by analyzing the data of the absorbed light, and finally, the blood sample is detected through the model, and the hemoglobin concentration is obtained and output. Compared with the prior art, under the condition that a blood sample is not pretreated, the hemoglobin concentration can be accurately detected, and a result is obtained; the method comprises the following steps: adding an oxygen-containing solution into a blood sample; compared with the prior art, by changing the light absorption property of hemoglobin in the blood sample, the hemoglobin can be detected more easily, and the detection accuracy is improved.

The invention discloses a floating algae flow type microimaging sheath fluid focusing micro-fluidic chip device and system, and belongs to the technical field of micro-fluidic control. According to the device, the three-channel sheath fluid flow chip design is adopted, a large-channel inlet structure is combined to avoid algae cell blockage, and a sheath flow focusing technology is utilized to compress a sample flow to the center of a channel and limit the sample flow within the field depth range of a microscope objective to obtain a clear image. The arc-shaped structure of the sample channel reduces laminar flow disturbance, and a four-side transparent observation area supports panoramic depth imaging. The system comprises a microscopic imaging module, a fluid driving module and a sheath liquid flow chip module, a flow rate ratio of sheath liquid flow to sample flow is accurately controlled through a dual-channel liquid flow driving injection pump, and stable imaging within the depth of field of an objective lens is realized by combining a manual focusing platform. Experimental verification shows that the system can effectively compress sample flow to 21m nuclear flow diameter, imaging of floating algae such as chlorella, scenedesmus quadricauda and the like is clear, and the contradiction between flux and imaging quality of a traditional micro-fluidic chip is solved.

The invention discloses a split type micro-fluidic chip and a working method thereof. In the chip, a reagent storage module is connected with a reaction module through a connection module, and the reaction module is slidably arranged in a chute of the connection module; a plurality of solution containing cavities are formed in the reagent storage module, a sealing film is attached to the top of the reaction module, and a plurality of solution overflowing cavities are formed in the reaction module; during work, the reaction module and the reagent storage module abut against each other, and when the solution containing cavity is communicated with the atmosphere, directional flowing and releasing of a solution in the solution containing cavity in the micro-fluidic chip to the solution overflowing cavity are achieved through negative pressure pumping. Sample sampling, cleaning, nucleic acid adsorption, elution and reaction in the micro-fluidic chip can be achieved, and instant detection of pathogenic microorganisms is achieved.

The present invention belongs to the field of microbead preparation technology, and specifically relates to a microbead production process with adjustable internal phase based on microfluidic technology and its application. The microbead production process includes the following steps: (1) raw material transportation: the required materials are transported to the microfluidic chip separately through a pump under heat preservation conditions; (2) logistics shearing: the required materials are sheared in the microfluidic chip to obtain composite droplets; (3) cooling and molding: after the sheared droplets are cooled, they are cooled and solidified using a cooling medium to form microbead particles; (4) product collection: the microbead particles and the cooling medium are subjected to solid-liquid separation, filtered, and collected to obtain microbeads. The microbead production process of the present invention can quickly realize the continuous production of double-layer microbeads, realize the precise regulation of the inner layer of the double-layer microbeads, and quickly and accurately control the particle size of the microbeads; at the same time, it can quickly realize the production conversion between single-layer microbeads and double-layer microbeads.

The invention provides a preparation method of a bionic microfluidic device for researching fluid migration in carbonate rock micro-nano fractures, which comprises the following steps: step 1, analyzing and classifying scanning electron microscope images of carbonate rock core slices through a random forest algorithm to generate a chip design drawing; step 2, based on the chip design drawing, re-etching a crack structure through a photoresist template and polydimethylsiloxane by adopting a soft lithography technology, and bonding with a glass substrate to form a micro-fluidic chip; and step 3, injecting a precursor liquid containing the calcite nano seed crystal into the micro-fluidic chip flow channel, fixing the seed crystal through ultraviolet irradiation, and injecting a saturated equilibrium liquid to realize calcium carbonate mineral precipitation so as to form the bionic micro-fluidic device. The method can be used for coupling research of all fluids and carbonate rock fractures, and a method for recovering the carbonate rock fracture structure to the maximum extent and a rock surface property cloning method are provided.

The invention relates to a wearable sensing device for sweat calcium ion detection. The wearable sensing device comprises a micro-fluidic chip and a signal processing and transmission module which can be matched with the sensing chip and is used for signal transmission and data processing, the micro-fluidic chip comprises a Janus fabric layer, a channel layer and a chip cover layer printed with an electrochemical electrode which are sequentially arranged in an attached manner; rapid sweat sampling and concentration refreshing can be realized, and the concentration of calcium ions in sweat can be directly and selectively measured; and the signal processing and transmission module is electrically connected with an electrochemical electrode for detecting the calcium ion concentration in the micro-fluidic chip and is used for data processing and transmission. By using a micro-fluidic chip, sweat secreted by skin continuously flows through an interface of the electrochemical sensor, so that the concentration of calcium ions in the sweat can be detected in real time; the sweat sample is small in size, detected sweat can be rapidly discharged through the micro-channel and the waste liquid discharging area, sweat accumulation is avoided, and the testing accuracy is guaranteed.

The invention provides a micro-fluidic chip for combined detection of C-reactive protein and ferritin and application thereof.The micro-fluidic chip comprises a substrate and a cover plate pressed on the substrate, a micro-channel is defined by the substrate and the cover plate, the left end of the micro-channel is communicated with a buffer solution sample adding hole formed in the cover plate, the right end of the micro-channel is communicated with a waste liquid area, and the left end of the micro-channel is communicated with a buffer solution sample adding hole formed in the cover plate. The waste liquid area is provided with a movable water-absorbing paper sheet, the microchannel is sequentially provided with a marking area, a detection area and a sample adding area from left to right, the marking area is provided with a marked anti-human CRP antibody, a marked anti-human SF antibody, a marked second antibody and a marked antigen, the first detection site is provided with an anti-human CRP antibody, and the second detection site is provided with an anti-human SF antibody. The first reference point is provided with a first antibody which reacts with the labeled second antibody, the second detection site is provided with an anti-human SF antibody, and the second reference point is provided with an antibody which reacts with the labeled antigen. The detection process of C-reactive protein and ferritin can be simplified, and the detection time is shortened.

The utility model discloses a micro-fluidic chip imaging positioning device, and belongs to the technical field of micro-fluidic chip positioning. According to the technical scheme, the system comprises a microcosmic illumination module, a microcosmic imaging module, a chip moving and macroscopic illumination module, a macroscopic imaging module and an optical path multiplexing module; the microscopic illumination module comprises a fly's-eye lens assembly, the fly's-eye lens assembly comprises two fly's-eye lenses, the optical axes of the two fly's-eye lenses are parallel to each other, and the focus of each lens array element in one fly's-eye lens coincides with the center of the lens array element at the corresponding position of the other fly's-eye lens. The micro-fluidic chip clamp is applied to the aspect of micro-fluidic chip imaging positioning, overcomes the defects caused by an existing micro-fluidic chip clamp, solves the technical problems of universality, automation and high precision of micro-fluidic chip positioning, and has the characteristics of rapidness, automation, high precision and universality.

The invention relates to the technical field of laser welding of micro-fluidic chips, in particular to a method and device for laser welding of a micro-fluidic chip. The method for laser welding of the micro-fluidic chip comprises the following steps: positioning the micro-fluidic chip, acquiring a local picture of the micro-fluidic chip through a visual detection module, controlling two end parts to automatically adjust positions on a space coordinate axis, and ensuring that a cover plate is tightly attached to the micro-fluidic chip; and the target heating light spot is aligned with the molten pool part, so that the matching degree of a real-time crystal phase image formed on the molten pool part and a target crystal phase image is larger than a set value, the welding procedure is completed in an automatic control and detection mode, and finally annealing is conducted to complete welding. The welding method is high in automation degree, laser welding is conducted through the laser welding head module, heating is fast, efficiency is high, and accurate and automatic welding is achieved by judging a crystal phase image.

Aspects of the present invention relate to a pancreatic cancer organoids on a chip device including a microfluidic chip having a top and bottom surface and a thickness therebetween, a central chamber embedded in the microfluidic chip, at least one opening in the top surface of the chip fluidly connected to the central chamber with one or more channels, and a plurality of evenly spaced micropillars arranged in a substantially circular shape within the central chamber such that the central chamber is partitioned into at least a first inner region and a first outer region, wherein the first inner region includes an immunocompetent pancreatic region configured to mimic a pancreatic niche, and the first outer region includes a vascular region configured to mimic an adjacent vascular network.

The invention discloses a method for determining the purity of electronic-grade hexafluoroethane, and relates to the technical field of the purity of hexafluoroethane, and the method comprises the following steps: vacuumizing a specific sampling container to 10 <-3 > Pa to 10 <-2 > Pa, forming a super-hydrophobic coating on the inner wall, sampling in a temperature-controlled, humidity-controlled and pressure-controlled environment, and removing water and impurities through two-stage pretreatment; enhancing analysis by using a gas chromatograph, a specific carrier gas, a column temperature program, a micro-fluidic chip and an ion mobility spectrometry; then secondary scanning analysis is carried out by using a mass spectrometer double ion source and a calibration system; and finally, learning and matching an algorithm through a data processing system machine. Samples can be accurately collected and pretreated, gas chromatography and mass spectrometry analysis is more accurate and efficient, a data processing system can accurately determine impurities and purity, multiple measurement data can be counted and analyzed, the result reliability is improved, all parts of the system are optimized and matched, the accuracy and stability of the whole process are guaranteed, and the purity is calculated; the method further comprises statistical analysis of multiple measurement data.

The utility model provides a micro-fluidic chip and a micro-fluidic system, a micron-sized main channel is arranged in the micro-fluidic chip, and a sample to be detected is introduced into a generation area of the main channel to form a large number of micro-droplets. The micro-droplets serve as a reaction container to react with tyrosine and tyrosinase which are guided into the reaction area of the main channel, and finally absorbance testing can be conducted on the micro-droplets in the detection area of the main channel. The micro-droplets are small in volume, have high surface area to volume ratio, and can form a reaction container for efficient reaction. The microfluidic system can rapidly and accurately measure to-be-detected samples with different concentrations, and has the advantages of low reagent consumption, energy conservation, environmental protection, small required detection space, large-scale parallel operation and the like.