3313 results about "Microfluidic chip" patented technology

The invention provides an agricultural environment monitoring method and system based on the Internet of Things. According to the method, multi-source data weights are dynamically distributed through a soil sensor array connected with Internet of Things nodes, and a global soil parameter set is generated; deploying a micro-electro-mechanical micro-fluidic chip in the coverage area to carry out soil solution selective permeation, converting the target ion concentration into an electric signal, and synchronously transmitting the electric signal and soil parameters; historical time series data are extracted, coherence is established through time correlation analysis, and undeployed area data are filled in combination with a spatial interpolation algorithm to construct a dynamic prediction model; according to the nutrient space change trend output by the model, crop growth requirements are matched to generate a fertilization amount adjustment instruction, and the fertilization amount adjustment instruction is issued to field fertilization equipment through the Internet of Things to execute dynamic regulation. Space-time precise sensing of soil nutrients and self-adaptive fertilization regulation and control are realized, and the utilization efficiency of agricultural resources and crop growth sustainability are improved.

The invention relates to the field of chip packaging testing, in particular to an automatic packaging testing method and system for a micro-fluidic chip. The method comprises the following steps: acquiring a real-time monitoring image of a micro-fluidic chip to be packaged in a packaging box; performing chip position alignment calibration processing on the real-time monitoring image of the micro-fluidic chip, and collecting a calibrated chip monitoring image; performing chip internal component visual inspection and multi-channel flow topological correlation fitting on the calibrated chip monitoring image, and constructing a chip packaging micro-channel topological structure model; and obtaining a packaging demand log, performing automatic packaging and filling execution on the chip packaging micro-channel topological structure model, and collecting environment monitoring parameters of the microfluidic packaging box. The packaging technology is continuously optimized through real-time feedback, the packaging test parameters are automatically adjusted, and the stability and precision of the packaging technology are gradually improved.

The invention discloses an intelligent detection method for water pollutants, which comprises the following steps of: transmitting a signal of a specific frequency band to a water body through an electromagnetic wave transmitting module, acquiring reflected and refracted signals by using a multi-channel receiver, extracting the characteristics of dielectric constant, refractive index and the like of the water body, and analyzing the types and the concentration range of the pollutants by adopting a pattern recognition algorithm; the method comprises the following steps: selecting a micro-fluidic chip and an electrochemical unit, selecting a corresponding biosensor for targeted detection, processing detection data through the micro-fluidic chip and the electrochemical unit, predicting the concentration, spatial distribution and trend of pollutants through a data analysis module, setting a dynamic self-adaptive threshold by the system, realizing pollution early warning, and uploading data to a cloud platform through a remote communication module. And remote monitoring is supported. The method can be widely applied to the fields of water quality monitoring, environmental protection and pollution control, and the detection precision, the real-time performance and the intelligent level are improved.

The invention relates to the technical field of fertilizer preparation, and discloses an ecological balance fertilizer for yield increase and disease resistance of peanuts and a preparation method of the ecological balance fertilizer. The preparation process comprises the following steps: constructing a PLGA core containing trichoderma harzianum spores and magnesium carbonate through a micro-fluidic chip low-temperature water-in-oil emulsion method; coating bacillus amyloliquefaciens and potassium silicate with ultraviolet-induced cross-linked temperature-sensitive hydrogel to form a middle layer; loading rhizobium by using aminated mesoporous S < iO2 >, and spraying and assembling an enzyme response shell through a fluidized bed; finally, the hydroxyapatite nanorod and a binder are combined and granulated and formed through a fluidized bed. Precise controlled release of functional bacteria and nutrients is realized by constructing a temperature-sensitive-enzyme response type release system, and the activity of a fungicide is guaranteed to be preserved by virtue of trichoderma harzianum-rhizobium synergistic interaction and silicon bond linkage soil remediation in combination with a low-temperature fluidized bed process, so that a'fertilizer-bacterium-soil 'ecological cycle system is finally constructed.

Provided are a double-cavity micro-fluidic chip having a reaction cavity and a test cavity and used for nucleic acid test, and a test method thereof. A fluid channel is provided between the reaction cavity and the test cavity, and only one exhaust channel is provided. The combination of the fluid channel and the exhaust channel can automatically provide resistance for a sample entering the test cavity from the reaction cavity, thereby achieving a controllable transfer of the sample between the reaction cavity and the test cavity. A gravity micro-fluidic chip and a centrifugal micro-fluidic chip are designed. The gravity micro-fluidic chip has a multi-turning circuitous fluid channel of which the diameter is smaller than that of a sample injection channel, such that under the action of gravity, the sample can flow into a reaction cavity from a sample adding region but cannot flow into the fluid channel, and then under pressurization by a simple pressurization device, the sample can enter a test cavity from the reaction cavity. The centrifugal micro-fluidic chip provides an exhaust channel branched from a fluid channel, and makes the exhaust direction opposite to a rotary centrifugal direction and the position of an exhaust opening higher than that of the fluid channel; therefore, by combining the principle of internal and external balance of the atmospheric pressure and the centrifugal driving performance, the centrifugal micro-fluidic chip effectively controls the flowing of a gas and a liquid in reaction cavities and test cavities, so as to effectively control the amount of the sample entering each cavity, so that multiple nucleic acid tests can be completed simply by means of one or two times of centrifugation. In addition, for a chip which only has a reaction cavity, an exhaust channel is connected to a liquid storage cavity, and a gravity-driving chip and an asymmetric pressure-driving chip are designed, thereby effectively avoiding aerosol pollution.

The invention discloses a dynamic optimization system and method for a compound essential oil ratio, and aims to solve the problems that the traditional technology depends on artificial experience, the optimization efficiency is low and the quality tracing is insufficient. According to the method, raw material molecular fingerprint data are collected in real time through a high-precision spectrum sensor array and a micro-fluidic chip, a production environment field domain is constructed in combination with a computational fluid mechanics model, a formula optimization problem is encoded into an Ising model by using a quantum annealing algorithm, and global search is realized through a quantum tunneling effect. The system integrates digital twin bodies, covers molecular dynamics simulation, phase equilibrium prediction and olfactory receptor activation probability simulation, and accurately maps a physical system state; a multi-modal data fusion network and a reinforcement learning framework are adopted to dynamically adjust formula parameters, and an NSGA-III algorithm is combined to optimize efficacy collaboration degree, cost and stability indexes. And the execution control module realizes nanoscale flow regulation through the piezoelectric micro-injection valve array, and realizes non-tampering evidence storage of formula parameters and quality data based on a block chain technology. Dynamic closed-loop optimization of the compound essential oil ratio is achieved, the production efficiency, the product consistency and the supply chain transparency are remarkably improved, and the method is suitable for the field of intelligent manufacturing of high-end essential oil.

The invention relates to the technical field of model analysis, in particular to an imaging flow cytometry cell detection method based on an improved model. The method comprises the following steps: introducing a cell sample to be detected into an imaging flow cytometry system integrated with a micro-fluidic chip for continuous image acquisition to generate an initial cell image sequence; an automatic digital focusing algorithm is applied to the initial cell image sequence, and a cell image frame set with the optimal focal plane is screened out; inputting the cell image frame set into a preset PA-YOLO improved model for multi-dimensional extraction and fusion, and generating a multi-scale cell characteristic spectrum; carrying out refined feature learning and cell target positioning and classification on the multi-scale cell feature spectrum, and outputting a cell detection result; and carrying out validity verification on the cell detection result, and carrying out comparative analysis in combination with an imaging flow cytometry system to generate a cell detection report. According to the method, the imaging quality and the detection accuracy of cell images with different depths can be remarkably improved.

The invention relates to the field of microfluid chemical synthesis, and discloses a method for preparing gold nanoparticles based on two-phase flow microfluid control. The method comprises the following steps: 1) converging a gold precursor water phase, a reducing agent water phase and an organic phase in a micro-fluidic chip at 0-25 DEG C to form water-in-oil liquid drops; (2) conveying the fluid to a mixing area channel of a micro-fluidic chip, and carrying out instantaneous nucleation reaction to form a gold nanoparticle prototype; 3) conveying the fluid into a capillary reactor, and further nucleating, growing and curing the gold nanoparticle prototype; and (4) the fluid is subjected to phase splitting, and a gold nanoparticle solution is obtained. A three-in-one micro-fluidic chip is adopted, process parameters are optimized, and a gold precursor and a reducing agent are wrapped in an organic phase before reaction to form water-in-oil liquid drops, so that on one hand, the situation of adsorption and scaling of the inner wall of a channel can be avoided, and on the other hand, the heat and mass transfer efficiency of a material can be improved through the generated liquid drops; and the uniformity of a reaction nucleation environment is ensured.

Embodiments of the invention include additional methods and compositions for fabricating organoids. A unique approach has been developed for the rapid aggregation of defined number of cells using a micropillar-based template to limit the size of organoids, which are then more commonly referred to as spheroids. This procedure can be utilized to create in vitro cardiac tissue models (e.g., cardiac organoids) simply, rapidly, and inexpensively. In certain aspects an alginate-gelatin based hydrogel premixed with cardiomyocytes is used in combination with a patterned microfluidic chip / cell culture chamber having an array of micropillars to fabricate 3D cardiac organoids.

The invention discloses a floating algae flow type microimaging sheath fluid focusing micro-fluidic chip device and system, and belongs to the technical field of micro-fluidic control. According to the device, the three-channel sheath fluid flow chip design is adopted, a large-channel inlet structure is combined to avoid algae cell blockage, and a sheath flow focusing technology is utilized to compress a sample flow to the center of a channel and limit the sample flow within the field depth range of a microscope objective to obtain a clear image. The arc-shaped structure of the sample channel reduces laminar flow disturbance, and a four-side transparent observation area supports panoramic depth imaging. The system comprises a microscopic imaging module, a fluid driving module and a sheath liquid flow chip module, a flow rate ratio of sheath liquid flow to sample flow is accurately controlled through a dual-channel liquid flow driving injection pump, and stable imaging within the depth of field of an objective lens is realized by combining a manual focusing platform. Experimental verification shows that the system can effectively compress sample flow to 21m nuclear flow diameter, imaging of floating algae such as chlorella, scenedesmus quadricauda and the like is clear, and the contradiction between flux and imaging quality of a traditional micro-fluidic chip is solved.

The present invention belongs to the field of microbead preparation technology, and specifically relates to a microbead production process with adjustable internal phase based on microfluidic technology and its application. The microbead production process includes the following steps: (1) raw material transportation: the required materials are transported to the microfluidic chip separately through a pump under heat preservation conditions; (2) logistics shearing: the required materials are sheared in the microfluidic chip to obtain composite droplets; (3) cooling and molding: after the sheared droplets are cooled, they are cooled and solidified using a cooling medium to form microbead particles; (4) product collection: the microbead particles and the cooling medium are subjected to solid-liquid separation, filtered, and collected to obtain microbeads. The microbead production process of the present invention can quickly realize the continuous production of double-layer microbeads, realize the precise regulation of the inner layer of the double-layer microbeads, and quickly and accurately control the particle size of the microbeads; at the same time, it can quickly realize the production conversion between single-layer microbeads and double-layer microbeads.

The invention relates to a wearable sensing device for sweat calcium ion detection. The wearable sensing device comprises a micro-fluidic chip and a signal processing and transmission module which can be matched with the sensing chip and is used for signal transmission and data processing, the micro-fluidic chip comprises a Janus fabric layer, a channel layer and a chip cover layer printed with an electrochemical electrode which are sequentially arranged in an attached manner; rapid sweat sampling and concentration refreshing can be realized, and the concentration of calcium ions in sweat can be directly and selectively measured; and the signal processing and transmission module is electrically connected with an electrochemical electrode for detecting the calcium ion concentration in the micro-fluidic chip and is used for data processing and transmission. By using a micro-fluidic chip, sweat secreted by skin continuously flows through an interface of the electrochemical sensor, so that the concentration of calcium ions in the sweat can be detected in real time; the sweat sample is small in size, detected sweat can be rapidly discharged through the micro-channel and the waste liquid discharging area, sweat accumulation is avoided, and the testing accuracy is guaranteed.

The invention provides a micro-fluidic chip for combined detection of C-reactive protein and ferritin and application thereof.The micro-fluidic chip comprises a substrate and a cover plate pressed on the substrate, a micro-channel is defined by the substrate and the cover plate, the left end of the micro-channel is communicated with a buffer solution sample adding hole formed in the cover plate, the right end of the micro-channel is communicated with a waste liquid area, and the left end of the micro-channel is communicated with a buffer solution sample adding hole formed in the cover plate. The waste liquid area is provided with a movable water-absorbing paper sheet, the microchannel is sequentially provided with a marking area, a detection area and a sample adding area from left to right, the marking area is provided with a marked anti-human CRP antibody, a marked anti-human SF antibody, a marked second antibody and a marked antigen, the first detection site is provided with an anti-human CRP antibody, and the second detection site is provided with an anti-human SF antibody. The first reference point is provided with a first antibody which reacts with the labeled second antibody, the second detection site is provided with an anti-human SF antibody, and the second reference point is provided with an antibody which reacts with the labeled antigen. The detection process of C-reactive protein and ferritin can be simplified, and the detection time is shortened.

The utility model discloses a micro-fluidic chip imaging positioning device, and belongs to the technical field of micro-fluidic chip positioning. According to the technical scheme, the system comprises a microcosmic illumination module, a microcosmic imaging module, a chip moving and macroscopic illumination module, a macroscopic imaging module and an optical path multiplexing module; the microscopic illumination module comprises a fly's-eye lens assembly, the fly's-eye lens assembly comprises two fly's-eye lenses, the optical axes of the two fly's-eye lenses are parallel to each other, and the focus of each lens array element in one fly's-eye lens coincides with the center of the lens array element at the corresponding position of the other fly's-eye lens. The micro-fluidic chip clamp is applied to the aspect of micro-fluidic chip imaging positioning, overcomes the defects caused by an existing micro-fluidic chip clamp, solves the technical problems of universality, automation and high precision of micro-fluidic chip positioning, and has the characteristics of rapidness, automation, high precision and universality.

The utility model provides a micro-fluidic chip and a micro-fluidic system, a micron-sized main channel is arranged in the micro-fluidic chip, and a sample to be detected is introduced into a generation area of the main channel to form a large number of micro-droplets. The micro-droplets serve as a reaction container to react with tyrosine and tyrosinase which are guided into the reaction area of the main channel, and finally absorbance testing can be conducted on the micro-droplets in the detection area of the main channel. The micro-droplets are small in volume, have high surface area to volume ratio, and can form a reaction container for efficient reaction. The microfluidic system can rapidly and accurately measure to-be-detected samples with different concentrations, and has the advantages of low reagent consumption, energy conservation, environmental protection, small required detection space, large-scale parallel operation and the like.

The present invention relates to a microfluidic multiplexed immunoassay system based on flow magnet-activated cell sorting, and a method thereof. The system comprises a multiplexed immunoassay magnetic bead preparation module, a flow magnet-activated cell sorting microfluidic chip system, an optical detection module, and an image processing module, and achieves ultra-sensitive and rapid multiplexed protein quantitative detection by means of magnetic bead coding based on different magnetic contents and microfluidic chip decoding based on flow magnet-activated cell sorting. The microfluidic multiplexed immunoassay system of the present invention has the advantages of high detection sensitivity, short detection time, high signal-to-noise ratio, convenient and simple operation and low cost, is particularly suitable for multiplexed ultra-sensitive detection for monomolecular proteins, and has a potential economic value.

The invention discloses a generative odor real-time synthesis method and system based on cross-modal potential space mapping, and belongs to the technical field of artificial intelligence and olfaction calculation. The method comprises the following steps: acquiring a multi-modal input stream of a current scene, and extracting an emotion semantic feature vector by using a deep neural network; mapping the semantic features into target odor chemical feature vectors by using nonlinear projection through a pre-constructed vision-smell joint embedding space; constructing a convex optimization model based on olfactory perception, and calculating a basic liquid optimal mixing proportionality coefficient matrix capable of fitting the target vector; the matrix is converted into a micro-fluidic driving signal, and the target smell is synthesized in situ in the micro-fluidic chip. The invention further discloses a self-adaptive cleaning logic and olfactory fatigue compensation mechanism based on scene mutation detection. The method solves the problems that in the prior art, label matching is dependent, new smell cannot be synthesized, and dynamic transition is lacked, and olfactory replicating and real-time generation of abstract semantic scenes are achieved.

The invention belongs to the technical field of microbead preparation, and particularly relates to a microbead production process with an adjustable internal phase based on a microfluidic technology and application of the microbead production process. The micro-bead production process comprises the following steps: (1) conveying raw materials: respectively conveying required materials to a micro-fluidic chip through a pump under a heat preservation condition; (2) material flow shearing: shearing the required material in the micro-fluidic chip to obtain composite liquid drops; (3) cooling and forming: cooling and curing the sheared liquid drops by using a cooling medium after the sheared liquid drops are cooled, so as to form microbead particles; and (4) product collection: carrying out solid-liquid separation on the microbead particles and a cooling medium, filtering, and collecting to obtain the microbeads. According to the microbead production process disclosed by the invention, continuous production of double-layer microbeads can be quickly realized, accurate regulation and control of inner layers of the double-layer microbeads are realized, and the particle sizes of the microbeads are quickly and accurately controlled; and meanwhile, the production conversion of single-layer microbeads and double-layer microbeads can be quickly realized.

Disclosed in the present invention are a microfluidic chip and a microfluidic device comprising same. The microfluidic chip comprises a microfluidic substrate, wherein a micro-channel structure is provided on the surface of the microfluidic substrate, and the micro-channel structure sequentially comprises a liquid-intake flow channel, a mixing flow channel and a liquid-output flow channel, which are in communication with each other; a first-stage mixing flow channel and a second-stage mixing flow channel are sequentially provided on the mixing flow channel, and the second-stage mixing flow channel comprises a flow-dividing channel and a flow-converging channel; the flow-dividing channel comprises two flow-dividing portions, which are arranged at an angle, input ends of the two flow-dividing portions are in communication with each other, and an inlet of the second-stage mixing flow channel is formed at the position where the flow-dividing portions are in communication with each other; and the flow-converging channel is arc-shaped, two input ends of the flow-converging channel are respectively in communication with output ends of the two flow-dividing portions, and an outlet of the second-stage mixing flow channel is formed at the most downstream part of the flow-converging channel. By means of sequentially providing two stages of mixing flow channels on the mixing flow channel, a solution that involves dividing a medium and then mixing same by means of frontal counter-collision is provided, such that, by means of a medium collision mixing principle, the degree of mixing is effectively improved and the preparation efficiency is also improved.

The present invention belongs to the field of organoid chips. Provided is a multiple-organoid co-culture micro-fluidic chip, comprising organoid micro-fluidic chip cells, each organoid micro-fluidic chip cell being divided into an upper layer and a lower layer which are bonded to each other, the upper layer being sequentially provided with an organoid culture medium perfusion channel, a first channel barrier, an organoid culture chamber, a second channel barrier and a vascular endothelial culture channel, and the lower layer being provided with a micro-perfusion network channel. The chip ensures specific nutrition and microenvironment for each organoid and helps to maintain specific phenotypes for same. In addition, the chip can simulate in-vitro blood flowing, so as to perform signal communication and interaction between organoids, thereby fully simulating the in-vivo environment and interaction for multiple-organ diseases.

The utility model discloses a device for preparing a double-droplet microsphere. The device comprises a fluid injection unit, a liquid injection unit and a control unit, the micro-fluidic chip comprises a cover glass, one side of the cover glass is provided with a dispensing needle a, the dispensing needle a is connected with an internal phase glass capillary tube, the internal phase glass capillary tube is partially nested in an intermediate phase glass capillary tube, the intermediate phase glass capillary tube is partially nested in an external phase glass capillary tube, and the external phase glass capillary tube is connected with the external phase glass capillary tube. A dispensing needle b is vertically arranged above one end, which is overlapped with the inner phase glass capillary, of the intermediate phase glass capillary, and a dispensing needle c is vertically arranged above one end, which is overlapped with the intermediate phase glass capillary, of the outer phase glass capillary; and a micro-droplet collection unit. According to the utility model, through the coaxially arranged glass capillary tube and the dispensing needle head communicated with the glass capillary tube, the double-droplet microsphere is prepared, meanwhile, the groove is formed in the dispensing needle head, so that the construction of a coaxial glass capillary tube micro-fluidic chip is facilitated, the preparation equipment is simple, and the operation is simple.

The invention relates to an organ-like core-shell microsphere as well as a preparation method and application thereof. The preparation method comprises the steps that 1, raw materials are prepared, specifically, shell raw materials and inner core raw materials are prepared, the shell raw materials comprise a photoinitiator and methacrylated hyaluronic acid (HAMA), and the inner core raw materials comprise organoid precursor cell suspension and matrigel; 2) molding: extruding the dispersion phase of the shell raw material wrapping the core raw material to a continuous phase by using a micro-fluidic chip and a high-precision injection pump, cutting the dispersion phase into liquid drops by the continuous phase, and performing illumination curing on the liquid drops to form core-shell microspheres; and (3) incubating, namely culturing the core-shell microspheres in a culture medium, so that the organoid precursor cells in the inner core are developed into organoids. According to the preparation method, rapid forming and curing of the organ-like precursor cells carried by the matrigel are achieved, so that the time of the organ-like precursor cells staying in the oil phase is shortened, the cells can obtain oxygen and nutrient substances, and the cell activity is improved.

The invention discloses a nano-drug preparation system and a nano-drug preparation process. The nano-drug preparation system comprises a micro-fluidic chip, at least two feeding devices and a controller, the two feeding devices are communicated with the micro-fluidic chip and are respectively used for conveying different raw material liquids; each feeding device comprises a liquid path reversing coordination integrated block and two sucking and pushing mechanisms; the liquid path reversing coordination integrated block is provided with an input end and an output end; the two sucking and pushing mechanisms are both communicated with a fluid channel of the liquid path reversing coordination integrated block, each sucking and pushing mechanism achieves input or output of raw material liquid in a sucking or pushing mode, the driving ends of the two sucking and pushing mechanisms are both in signal connection with the controller, and the controller is used for controlling the two sucking and pushing mechanisms to alternately operate in the reverse direction; a one-way valve assembly is arranged in the liquid path reversing coordination integrated block. By means of the arrangement, the function of stably and continuously conveying raw material liquid without liquid mixing is achieved, the controllability of the preparation process of nano-drugs in the micro-fluidic chip is guaranteed, and the purpose of improving the preparation effect is achieved.

The invention relates to the technical field of medical instruments, in particular to an intelligent wound nursing system which comprises a dressing body, and the dressing body comprises a skin-friendly layer, a functional layer and a protective layer. A monitoring module, a control module, a medicine leading-in module, a flexible circuit layer and an alarm module are integrated in the functional layer. According to the method, the infection misdiagnosis rate is reduced through multi-sensor fusion monitoring and a preset infection risk threshold algorithm; according to the design, the micro-fluidic chip and the biological probe are integrated in the percolate component sensor, so that the infection detection rate is increased; a temperature-sensitive hydrogel micro-valve array and nanofiber membrane slow release technology is adopted, so that medicine waste or poor treatment effect is reduced; through a multi-sensor data fusion algorithm, the detection accuracy is improved; the layout of the micro-valve array and the annularly distributed independent medicine storage cavities is adopted, so that the release error is reduced; through the ultrathin flexible framework, the snakelike wiring circuit and flip chip packaging, circuit breakage is avoided, and the clinical failure rate is reduced.

The present invention belongs to the technical field of microfluidic chips, and discloses a control valve which is arranged on a microfluidic channel (2) of a microfluidic chip. The microfluidic chip comprises a chip body (1). The control valve comprises a valve cavity (3) and a reversible water absorption mechanism (4), and the reversible water absorption mechanism (4) is arranged in the valve cavity (3). Also disclosed is a centrifugal microfluidic chip, which comprises a chip body (1), a liquid inlet (7), a reaction chamber (8), and a microfluidic channel (2), and further comprises a control valve arranged between the liquid inlet (7) and the reaction chamber (8), and the control valve is the above-mentioned control valve. The present invention provides a control valve and a centrifugal microfluidic chip, which are simple to process, do not require complex treatment, can realize the step-by-step release of a solution by changing the centrifugal speed, and are convenient to use.

The invention provides cell culture monitoring equipment which comprises a cell culture box and a monitoring module, the cell culture box is used for installing a micro-fluidic chip and further comprises a first observation window, the first observation window is used for exposing an observation area of the micro-fluidic chip, and the monitoring module comprises an imaging assembly. The imaging assembly is used for collecting an image of the micro-fluidic chip from the first observation window. The cell culture monitoring equipment can realize real-time and online monitoring of the cell growth process.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to a hot pressing method of a micro-fluidic chip and the micro-fluidic chip. The hot-pressing method of the micro-fluidic chip comprises the following steps: making a mold and preparing a forming mold, the forming mold comprises the following steps: preparing a female mold and at least two male molds, and the heights of the at least two male molds are different; detachably connecting the female die with one of the male dies; heating is conducted, specifically, the male die abuts against the blank, and the blank, the male die and the female die are heated; hot pressing is conducted, so that hot pressure exists between the male die and the blank, the male die extrudes the blank, the blank copies the shape of the male die, and the blank is machined into the micro-fluidic chip; demolding, cooling the micro-fluidic chip, the female mold and the male mold, and separating the micro-fluidic chip from the male mold. According to the invention, different micro-channel chips can be processed through one set of forming die, so that the preparation cost for preparing various different micro-channel chips is reduced.

The invention discloses an ultrasonic phased array driven graphene oxide flow stripping and closed-loop regulation and control system and method, and the system comprises an ultrasonic phased array module, and a piezoelectric transducer array element in the ultrasonic phased array module controls sound beam focusing and energy distribution through a phase delay controller, and carries out the stripping of graphite oxide flowing in a flow channel; a micro-Raman spectrometer in the micro-fluidic-Raman detection module collects characteristic peak data of a graphene oxide sheet layer in a micro-fluidic chip in real time and sends the characteristic peak data to a signal analysis unit; and the feedback control module is used for receiving the layer number distribution data output by the signal analysis unit, dynamically adjusting ultrasonic parameters of the phase delay controller, and controlling the diverter valve to guide substandard products into a secondary treatment channel and finally return the substandard products to the flow channel for stripping again. The system and the method for stripping the graphene oxide based on coupling of the ultrasonic phased array and the micro-fluidic chip have the advantages of efficient stripping, accurate detection, closed-loop optimization and the like.

The invention relates to a centrifugal micro-fluidic chip for automatic detection of a nano-enzyme test strip and application of the centrifugal micro-fluidic chip. The centrifugal micro-fluidic chip integrates the steps of sample adding, chromatography, color development and the like of the nano-enzyme test strip, and full automation of nano-enzyme experiments on the micro-fluidic chip is realized. Comprising a reaction structure main body, a bottom runner layer, a vertical through hole layer, a pressing layer, a top runner layer and a sealing body. The closed structure of the chip effectively reduces the interference of external environmental factors on the experiment, reduces the risk of sample pollution, and ensures the high reliability of the detection result.

The invention belongs to the technical field of microfluidics, and particularly relates to a liquid separation-convergence integrated liquid drop micro-fluidic chip and a liquid drop preparation method.The liquid separation-convergence integrated liquid drop micro-fluidic chip is characterized in that a liquid drop generation unit is arranged at the communication position of a branch splitting channel and a convergence channel which is correspondingly communicated with the branch splitting channel; and under the action of continuous phase shearing force, the dispersed phase solution is sheared off by the continuous phase solution, so that liquid drops are formed. The droplet generation units are symmetrically arranged along the circumference of the channel, the distance from each unit to the center is equal, and a continuous phase solution and a dispersed phase solution encountered at each droplet generation unit are almost equivalent, so that the sizes of the generated droplets are consistent, the droplets with basically consistent sizes are obtained at a collection port, and high-flux preparation of microspheres with uniform sizes is realized.