1334 results about "Microfluidic chip" patented technology

The invention relates to the technical field of model analysis, in particular to an imaging flow cytometry cell detection method based on an improved model. The method comprises the following steps: introducing a cell sample to be detected into an imaging flow cytometry system integrated with a micro-fluidic chip for continuous image acquisition to generate an initial cell image sequence; an automatic digital focusing algorithm is applied to the initial cell image sequence, and a cell image frame set with the optimal focal plane is screened out; inputting the cell image frame set into a preset PA-YOLO improved model for multi-dimensional extraction and fusion, and generating a multi-scale cell characteristic spectrum; carrying out refined feature learning and cell target positioning and classification on the multi-scale cell feature spectrum, and outputting a cell detection result; and carrying out validity verification on the cell detection result, and carrying out comparative analysis in combination with an imaging flow cytometry system to generate a cell detection report. According to the method, the imaging quality and the detection accuracy of cell images with different depths can be remarkably improved.

The invention discloses a generative odor real-time synthesis method and system based on cross-modal potential space mapping, and belongs to the technical field of artificial intelligence and olfaction calculation. The method comprises the following steps: acquiring a multi-modal input stream of a current scene, and extracting an emotion semantic feature vector by using a deep neural network; mapping the semantic features into target odor chemical feature vectors by using nonlinear projection through a pre-constructed vision-smell joint embedding space; constructing a convex optimization model based on olfactory perception, and calculating a basic liquid optimal mixing proportionality coefficient matrix capable of fitting the target vector; the matrix is converted into a micro-fluidic driving signal, and the target smell is synthesized in situ in the micro-fluidic chip. The invention further discloses a self-adaptive cleaning logic and olfactory fatigue compensation mechanism based on scene mutation detection. The method solves the problems that in the prior art, label matching is dependent, new smell cannot be synthesized, and dynamic transition is lacked, and olfactory replicating and real-time generation of abstract semantic scenes are achieved.

The invention belongs to the technical field of microfluidics, and particularly relates to a liquid separation-convergence integrated liquid drop micro-fluidic chip and a liquid drop preparation method.The liquid separation-convergence integrated liquid drop micro-fluidic chip is characterized in that a liquid drop generation unit is arranged at the communication position of a branch splitting channel and a convergence channel which is correspondingly communicated with the branch splitting channel; and under the action of continuous phase shearing force, the dispersed phase solution is sheared off by the continuous phase solution, so that liquid drops are formed. The droplet generation units are symmetrically arranged along the circumference of the channel, the distance from each unit to the center is equal, and a continuous phase solution and a dispersed phase solution encountered at each droplet generation unit are almost equivalent, so that the sizes of the generated droplets are consistent, the droplets with basically consistent sizes are obtained at a collection port, and high-flux preparation of microspheres with uniform sizes is realized.

The invention discloses a closed-loop control digital microfluidic system and a method thereof, and relates to the technical field of microfluidics, and the method comprises the following steps: initializing a digital microfluidic chip electrode array, setting an electrode spacing, an electrode size and a voltage upper limit, collecting a no-load state signal, and generating initial reference data; liquid drops are formed at the preset position of the digital micro-fluidic chip, and according to the initial reference data, the initial position, the volume and the form information of the liquid drops are collected, and a liquid drop initial state data set is generated; inputting the initial state data set of the liquid drop into a pre-trained closed-loop prediction model, performing kinetic analysis and trajectory prediction calculation, generating an expected motion trajectory of the liquid drop, and comparing the expected motion trajectory with the real-time state data flow of the liquid drop to generate deviation feedback data of the liquid drop; and dynamically adjusting the initial driving voltage parameter according to the droplet deviation feedback data to generate a closed-loop driving voltage sequence. The liquid drop trajectory control precision is improved, and motion errors caused by environmental disturbance and electrode response differences are reduced.

The invention discloses a detection kit and method for detecting allergic inflammatory factors based on a centrifugal micro-fluidic chip, and relates to the field of kits, the detection kit comprises the centrifugal micro-fluidic chip and a preset reagent; the centrifugal micro-fluidic chip comprises a chip base body, a sample adding cavity, a liquid level sensor, a liquid level sensor, a liquid level sensor, a liquid level sensor and a liquid level sensor, the nucleic acid extraction and purification cavity comprises a plurality of amplification cavities; a reagent storage chamber; a waste liquid chamber; and a capillary valve. The functions of nucleic acid extraction, variable temperature amplification and multi-target synchronous detection are integrated through the centrifugal micro-fluidic chip, full-closed operation is achieved by means of centrifugal force or other driving force such as air pressure and electroosmotic flow, and the pollution risk caused by manual step-by-step operation is thoroughly eliminated; a multi-wavelength distinguishable fluorescent probe and a grouping preset primer combination are adopted to synchronously complete parallel detection of the allergic inflammatory factor mRNA and the drug accompanying diagnosis target, cross interference is avoided, and large instruments and equipment do not need to be relied on.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to an integrated micro-fluidic card box. The device comprises a first solution storage part, an adsorption part, a second solution storage part and a reaction part, the bottom surface of the first solution storage part is rotationally connected with the top surface of the adsorption part, the bottom surface of the adsorption part is rotationally connected with the top surface of the second solution storage part, and the bottom surface of the second solution storage part abuts against the top surface of the reaction part; a second channel penetrates through the first solution storage part, a second runner is arranged on the adsorption part, and a second through hole penetrates through one end of the adsorption part; during single-sample nucleic acid detection, lysate feeding, cleaning liquid feeding, eluent feeding and sample loading share an exhaust channel formed by the second flow channel and the second channel, and the flow process of the solution can be controlled only by one driving pump; the driving pump and the external pipeline only discharge gas but not liquid, so that the solution is prevented from being remained when passing through the pump and the pipeline, and pollution is avoided; a plurality of micro-fluidic chips are communicated with the driving pump and the controller, so that a plurality of samples can be detected at the same time, and the detection efficiency is improved.

The invention discloses a dual-mode micro-droplet generation micro-fluidic chip and application thereof in generation of dual-emulsion droplets, and relates to the technical field of micro-fluidic chips. The method comprises the following steps: respectively injecting two different internal phase solutions from the left ends of respective iron tubes, guiding the internal phase solutions to a wedge-shaped tube orifice of a theta-shaped glass capillary tube through respective thin tubes, injecting an intermediate phase solution into a square glass tube through an intermediate phase inlet by using a No.1 needle, and injecting an external phase solution into the square glass tube through an external phase inlet by using a No.2 needle; the two internal phase solutions, the intermediate phase solution and the external phase solution converge at an inlet of the circular glass capillary tube, and after entering the circular glass capillary tube, water-in-oil-in-water double-emulsion droplets are obtained. The invention can obtain the dual-mode micro-droplet generation micro-fluidic chip and the application of the dual-mode micro-droplet generation micro-fluidic chip in generation of dual-emulsion droplets.

The invention relates to the field of microbiological detection, in particular to a method for detecting vibrio parahaemolyticus by combining RPA-CRISPR / Cas12a with a microfluidic biosensor. According to the method, the preloaded freeze-drying reagent is combined with centrifugally driven liquid operation, so that rapid and closed system detection is realized, and the pollution risk is effectively reduced. According to the invention, quantitative detection of vibrio parahaemolyticus is realized in a centrifugal micro-fluidic chip by systematically optimizing an RPA amplification primer and crRNA. The detection method shows high specificity and high sensitivity to non-target vibrio and other food-borne bacteria, and the lower detection limit is 6.08 copies / mu L. In a word, the invention provides a rapid, portable and sensitive platform, can realize quantitative detection in a wide linear range, provides a practical tool for real-time monitoring of pathogens, and has application prospects in monitoring of aquaculture biosafety and marine product safety.

The invention belongs to the field of biological microfluidics, and particularly relates to a detection device for biomarkers in blood. The invention provides a device for detecting biomarkers in blood. The device comprises a blood sampling layer, a detection layer and a micro-fluidic chip layer which are adjacent in sequence. According to the device for detecting the biomarkers in the blood, a blood sampling module, an anticoagulation module, a sample transmission module, a chemical reaction module and a signal detection module are integrated on a miniature wearable platform, and portable, painless, rapid and accurate detection is achieved.

The utility model relates to the technical field of microfluidics, and discloses a multi-stage micro-fluidic chip, which at least comprises a first-stage collision fluid channel and a second-stage collision fluid channel, and a mixing channel of the upper-stage fluid channel is arranged as a premixing fluid channel of the lower-stage fluid channel; the first-stage collision fluid channel comprises a first premixed fluid channel and a second premixed fluid channel, a first-stage fluid collision channel is arranged between the first premixed fluid channel and the second premixed fluid channel, and the first-stage fluid collision channel enters the second-stage premixed fluid channel after collision; a second-stage fluid collision channel is arranged between the third premixing fluid channel and the fourth premixing fluid channel, and the second-stage fluid collision channel enters the mixing channel after collision. The multi-stage micro-fluidic chip has the advantages of high mixing efficiency, low shear damage, easiness in integrated packaging and batch manufacturing and the like, and the micro-fluid mixing performance and the system practicability are remarkably improved.

The invention discloses a YOLO-Drop-based 3D printing droplet microfluidic immunoassay method, which organically integrates a high-precision microfluidic chip preparation technology, a droplet-level immunoreaction system and a fluorescence imaging acquisition and deep learning detection algorithm. And full-chain closed-loop processing from droplet generation, fluorescence signal acquisition, image recognition, result statistics to concentration quantitative analysis is realized. Through deep combination of chip physical parameters and model input features, the method not only significantly improves the accuracy and stability of droplet identification and concentration quantification, but also enhances the robustness of the algorithm under complex sample conditions through multi-scale feature fusion and a signal dual screening mechanism. Meanwhile, the optimized YOLO-Drop model supports embedded efficient reasoning, so that the whole analysis process has the characteristics of real-time performance and high throughput, and the method is suitable for rapid quantitative detection of various biomarkers.

The invention relates to the technical field of crossing of biomedical engineering, molecular diagnosis and nano-optical sensing, and particularly discloses a virus nucleic acid variation amplification-free LSPR (Local Surface Plasmon Resonance) detection method and chip based on a gold nano-structure. The virus nucleic acid variation amplification-free LSPR detection chip based on the gold nanostructure comprises a sample injection port, a reaction area, a washing area, a detection area and a waste liquid area, the sample injection port is used for injecting a viral nucleic acid or protein sample, a plurality of groups of probe sequences are fixed in different reaction regions of the chip, non-amplification rapid detection of viral nucleic acid variation is realized, high sensitivity and specificity are ensured, and by utilizing the unique advantages of an MSNs template branched gold nanostructure, the detection sensitivity is improved. Refractive index change caused by single base mutation is directly amplified, the capability of completing femtomole-level detection within 30 minutes is achieved, the overall detection speed is increased, rapid typing detection of viral nucleic acid variation is achieved through LSPR spectral signals under the non-amplification condition, and a portable platform is formed by integrating the LSPR spectral signals and a micro-fluidic chip.

The invention belongs to the technical field of microfluidics, and particularly relates to a microfluidic chip and a method for preparing liquid drops in a high-flux and large-scale manner. A step emulsification structure and a liquid drop splitting structure are used together, a liquid drop generation structure is arranged at the communication position of a branch splitting channel and a main channel unit correspondingly communicated with the branch splitting channel, a dispersion phase necks at the liquid drop generation structure under the action of interfacial tension to form liquid drops, and the liquid drops are generated under the leading of the interfacial tension, so that the dispersion phase is uniform in dispersion. Therefore, the size of the liquid drops is basically determined by the channel width of the liquid drop generation structure, the branch splitting channel is designed to be wide, the influence of manufacturing errors on the uniformity of the generated liquid drops can be reduced, the branch splitting channel is designed to be a plurality of serpentine channels, and the uniformity of the liquid drops is improved while high-flux liquid drop preparation is guaranteed; and then the liquid drops flow through the main channel unit and are converged into the main splitting channel, and the liquid drops are uniformly split into liquid drops with smaller sizes through the liquid drop splitting structure, so that the preparation of the liquid drops with high flux and uniform sizes is realized.

The invention relates to the technical field of microfluidics, in particular to a carbon quantum dot hydrothermal synthesis system and method based on microfluidics thermal field equilibrium, which comprises a thermal field sensing module, an equilibrium analysis module, a thermal field coupling module, an equilibrium regulation and control module and a closed loop execution module, the thermal field sensing module is used for collecting array temperature on the surface of the micro-fluidic chip and fluid pressure data in a flow channel, and reconstructing three-dimensional temperature distribution in the micro-channel through a multi-physics field coupling algorithm to obtain a real-time thermal field state matrix. According to the invention, by constructing a multi-physical field coupling algorithm of a thermocouple array-flow resistance model-conjugate heat transfer equation, the three-dimensional temperature distribution of the fluid in the micro-channel can be inverted and reconstructed by using surface temperature and pressure data, so that a control system can pass through a chip matrix, and the temperature of the fluid in the micro-channel can be controlled. The radial temperature gradient and the axial thermal distortion in the fluid are directly sensed, the hysteresis and the error of sensor measurement are eliminated, and a real physical basis is provided for accurate temperature control.

The invention discloses a preparation method of a microfluidic phase change cooling liquid, and belongs to the technical field of advanced thermal management material preparation. According to the method, a specific microfluidic flow and functional fluid are designed, and a molten phase-change material fluid, a shell fluid containing curable resin and a heat-conducting filler and a cooling base fluid insoluble in a shell are pumped into a microfluidic chip respectively. In a chip flow channel, under the action of hydrodynamic force and interfacial tension, shell layer fluid wraps phase change material fluid in situ, and monodisperse core-shell liquid drops are formed and dispersed in base fluid. And then guiding the liquid drops to pass through a curing area, so that the shell layer is cured in the flowing process, and a suspension system with the phase change microcapsules dispersed in the base solution is directly obtained. According to the invention, accurate control of the size of the phase change microcapsule and the thickness of the shell layer is realized, and the preparation method of the phase change cooling liquid which is continuous, efficient and controllable in structure is provided.

The invention belongs to the technical field of biological intelligent detection, and particularly relates to a circulating tumor cell automatic detection device based on a micro-fluidic nano chip and AI analysis and application. The device comprises a micro-fluidic chip module, an optical acquisition module, an image recognition module, a feedback control module and a liquid path control module, and can realize efficient enrichment, image recognition and dynamic fluidic regulation of circulating tumor cells in peripheral blood. By constructing a change trend model of an image frame sequence, cell state changes are recognized in real time, flow velocity and channel control instructions are output, and collaborative optimization of recognition precision and the sample utilization rate is achieved. The device is suitable for tumor early screening, curative effect monitoring and recurrence early warning scenes.

The invention discloses a numerical simulation method for preparing organic nanoparticles by microfluidics and a preparation method of the organic nanoparticles. The method comprises the following steps: S1, obtaining three microchannel structures with different forms; and S2, performing numerical simulation of the nucleation-growth-aggregation process of the organic nanoparticles in the micro-fluidic chip according to the three micro-channel structures with different forms. By adopting the technical scheme provided by the invention, the influence of structural parameters such as the structure, the flow velocity and the flow velocity ratio and hydrodynamic parameters on the particle size and uniformity of the organic nanoparticles is quantitatively analyzed through numerical simulation.

The invention discloses a micro-fluidic DNA detection-based security key generation and authentication method, which comprises the following steps: S1, carrying out DNA detection on a biological sample through a micro-fluidic chip to obtain SNP and STR feature data; s2, performing unified coding on the SNP and STR feature data to form stable bit string representation; s3, processing the bit string representation by using a fuzzy extraction mechanism, and generating a consistent key material under the condition of permitting a detection error; s4, deriving a final symmetric key from the key material by using a key derivation function; and S5, performing security packaging, transmission and authentication on the final symmetric key by adopting a post-quantum cryptography mechanism. According to the security key generation and authentication method based on microfluidic DNA detection, a set of key generation and identity authentication scheme with instantaneity, high specificity and anti-quantum security is constructed.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

The invention relates to the field of medical materials, and particularly discloses a collagen microsphere preparation method based on a micro-fluidic technology, and the preparation method comprises the following steps: preparing a micro-fluidic device which comprises a chip with a coaxial flow channel, a fluid control system and an ultraviolet crosslinking module integrated at the outlet of the chip; preparing an inner phase fluid, an intermediate phase fluid and an outer phase fluid, wherein the inner phase is a modified collagen solution containing a photoinitiator, the intermediate phase is a perfluoropolyether oil phase and contains a surfactant, and the outer phase is a water phase and contains a nonionic surfactant; the three-phase flow velocity is adjusted, and monodisperse W / O / W liquid drops are generated in the micro-fluidic chip; sequentially carrying out low-temperature physical crosslinking and ultraviolet-initiated crosslinking on the W / O / W liquid drops to form cured microspheres; collecting and post-processing the microspheres to obtain the collagen microspheres. According to the preparation method provided by the invention, the natural activity of collagen is reserved, meanwhile, the size of the microspheres is accurately controlled, multiple functions are integrated, and the preparation method is environment-friendly and safe.

An apparatus may include a microfluidic chip including at least one microfluidic channel and a first at least one electrical interconnect. An apparatus may include a circuit board having at least one interface interconnect and a second at least one electrical interconnect electrically coupled with the first at least one electrical interconnect of the microfluidic chip. An apparatus may include a cartridge enclosing the microfluidic chip and the circuit board, the cartridge including at least one opening allowing access to the at least one microfluidic channel on the microfluidic chip and the at least one interface interconnect.

The invention discloses a hydrogel microsphere sorting method, an artificial organ preparation method, a system and a medium. The method comprises the following steps: acquiring a hydrogel microsphere image in the micro-fluidic chip; analyzing the hydrogel microsphere image through a neural network to obtain an analysis result; based on the analysis result, hydrogel microspheres wrapping the target cells are sorted out; the neural network comprises a feature extraction layer and a multi-scale joint output head, and the hydrogel microsphere image is processed by the multi-scale joint output head after being convolved by the feature extraction layer to obtain an analysis result. According to the method, the hydrogel microspheres wrapped with the target cells are accurately identified and sorted by using a droplet microfluidic technology, a standardized artificial organ can be prepared based on the sorted hydrogel microspheres, and the functional maturity and reliability of the artificial organ are remarkably improved.

The invention relates to the technical field of micro-fluidic biological detection, and discloses a micro-fluidic chip for rapidly detecting bacteria based on multi-mode detection, a preparation method and a detection method.The chip comprises a PDMS runner layer and a glass substrate layer which are sequentially arranged from top to bottom; the PDMS flow channel layer comprises an injection area used for receiving a to-be-detected sample and carrying out pretreatment; the focusing area is used for realizing three-dimensional fluid focusing of the detected particles through a coupling effect of viscoelasticity and an inertial effect; the detection area is used for performing electrical impedance detection and fluorescence detection on the focused particles; the outflow area is used for collecting and outputting the detected sample; the PDMS flow channel layer and the glass substrate layer are bonded through oxygen plasma to form a sealed channel structure, and the sealed channel structure is used for realizing electrical impedance and fluorescence dual-mode synchronous detection of microbial particles in a single flow channel. By optimizing the fluid focusing structure and the electrode design, high-precision synchronous detection of the microbial particles is realized.

The invention relates to a three-dimensional multiple high-throughput analysis system and method based on microfluidics, and the system comprises a microfluidic chip integrating a temperature control reaction area and a detection area, an X-ray plate scanning module, a lens-free imaging unit, a synchronous control system and a data processing module. The device breaks through the bottleneck of plane limitation and spectrum crosstalk in the traditional technology, realizes high flux, three-dimensional space resolution and multi-parameter comprehensive analysis (multi-channel fluorescence + three-dimensional form + reaction kinetics), and has the characteristics of compact equipment, low cost, easiness in maintenance and the like; and an efficient and flexible analysis tool is provided for the fields of biomedical research, clinical diagnosis, drug screening and the like.

The invention discloses an immune quantitative detection device for tumor markers, relates to the technical field of medical detection, and aims to solve the problems of complicated operation, long detection period and poor portability in immune quantitative detection of tumor markers in the prior art. Comprising a shell, a disposable detection card, a multi-channel micro-fluidic chip, a high-sensitivity sensing system, a micro magnetic control incubation module, an AI auxiliary image recognition system and a wireless transmission module, the disposable detection card is used for accommodating the multi-channel micro-fluidic chip and a reaction reagent, and the multi-channel micro-fluidic chip is respectively aligned with the high-sensitivity sensing system and the micro magnetic control incubation module; the high-sensitivity sensing system is composed of a surface plasma resonance sensor and a quantum dot fluorescence labeling module, and the surface of a sensing chip of the surface plasma resonance sensor is modified with a tumor marker specific capture antibody. The method has the advantages of convenient detection and high efficiency and precision.

The utility model relates to a micro-fluidic flow cytometry fluorescence detector which comprises an excitation light source optical fiber, a light beam shaping module, a focusing and fluorescence collecting module and a micro-fluidic chip which are sequentially arranged along the excitation light path direction of the excitation light source optical fiber, and a multi-channel silicon photomultiplier tube is arranged above the micro-fluidic chip. A plurality of groups of detection channels for receiving fluorescence signals of different wave bands are arranged in the multichannel silicon photomultiplier tube at intervals up and down, and a dichroscope, a band-pass filter and a lens group are sequentially arranged in each group of detection channel along a light path and are gathered on a receiving chip; the micro-fluidic flow cytometry fluorescence detector can efficiently and accurately complete multi-wavelength fluorescence excitation and signal collection, and is suitable for multi-channel and multi-wavelength fluorescence detection requirements in a micro-fluidic chip.

The invention discloses a sweat induction and multi-parameter detection integrated sensor and a preparation method and application thereof, and belongs to the technical field of flexible biosensing, a PDMS flexible substrate is adopted to construct a multi-layer integrated structure, the sweat induction and multi-parameter detection integrated sensor comprises a sweat guide layer, a sweat collection layer, a detection electrode layer and a micro-fluidic chip layer, sweat secretion is promoted by accurately controlling the temperature to be less than or equal to 40 DEG C; the detection electrode layer adopts a silk-screen printing process to prepare a three-electrode system, comprises a specifically modified glucose working electrode and a beta-hydroxybutyric acid working electrode and can synchronously detect the concentration of glucose and beta-hydroxybutyric acid in sweat, and the micro-fluidic chip layer is integrated with a Bluetooth transmission module to realize real-time wireless transmission of detection data to a mobile terminal; the biosensor has the advantages of being good in biocompatibility, high in detection sensitivity and comfortable to wear and can tolerate bending with the curvature radius larger than 5 mm, and a non-invasive and continuous metabolism monitoring solution is provided for diabetes management.

The utility model discloses a micro-fluidic chip flow channel and a single-cell paired micro-fluidic chip, the micro-fluidic chip flow channel comprises a microsphere flow channel and at least one separation device, the microsphere flow channel is provided with a microsphere inlet and a microsphere outlet, the separation device is arranged in the microsphere flow channel, microspheres flow through the separation device in the process of flowing from the microsphere inlet to the microsphere outlet, and the microspheres flow through the single-cell paired micro-fluidic chip. The separation device is used for separating the microspheres, so that the microspheres which are relatively close to each other can be separated, gaps are formed among the microspheres, the microspheres can enter the liquid drop singly, and the phenomenon of empty package or multiple package of the liquid drop is avoided. And the separation device separates the microspheres through physical action, so that the stability is good, and the separation effect is good.

The invention relates to the technical field of micro-fluidic chips, and discloses a multi-fluid effect synergistic three-dimensional single-row focusing micro-fluidic chip and a method for single-line focusing of polystyrene microspheres by using the micro-fluidic chip, the micro-fluidic chip comprises a sample liquid inlet, one end of the sample liquid inlet is provided with an inertia focusing link, and the other end of the sample liquid inlet is provided with an inertia focusing link; a tapered flow guide cone structure is arranged between the inertial focusing link and the sample liquid inlet and is used for inhibiting the initial dispersion and diffusion trend of particles at the sample liquid inlet; the bionic gradient height structure is positioned at one end, far away from the tapered flow guide cone structure, of the inertial focusing link and is used for focusing and compressing the particles in the vertical direction; a sheath fluid focusing area is arranged at one end, far away from the inertial focusing link, of the bionic gradient height structure, and a sheath fluid inlet and a chip mixed liquid outlet are sequentially formed in one end, far away from the bionic gradient height structure, of the sheath fluid focusing area. According to the invention, efficient three-dimensional centered arrangement is realized, dynamic convergence of a particle trajectory from a dispersion state to a central single beam is realized, and a digital focusing regulation capability is realized.

The invention belongs to the technical field of micro-fluidic chips, and particularly relates to a micro-fluidic card box. The device comprises a first solution storage part, a puncturing part and a second solution storage part, a first limiting part and a fifth chamber are arranged in the first solution storage part; the top surface of the puncturing piece is a second limiting part, a third limiting part is arranged below the puncturing piece, a first groove is formed between the second limiting part and the third limiting part, a first channel, a second channel and a third channel which are communicated with one another are arranged in the puncturing piece, the third channel is communicated with the outside, a second groove is formed above the third channel, and a fourth limiting part and a solid spine part of a needle head are arranged below the third channel; a third groove is formed between the fourth limiting part and the third channel; on the second solution storage piece, the sixth cavity and the seventh cavity are communicated through a sixth channel, the sixth cavity and the eighth cavity are communicated through a fifth channel, and the inner diameter of the fifth channel is smaller than the diameter of the top face of the puncturing piece; according to the invention, the solution directionally flows into the target chamber in sequence through centrifugation, and mutual pollution is avoided in the transfer process.