19 results about "Mycoprotein" patented technology

The utility model provides a mycoprotein production system which is characterized by comprising a building body on which a steel frame platform is built; the slurry mixing tank is fixed on the building body; the plurality of fermentation tanks are fixed on the building body, and a second conveying pipe is arranged between the slurry mixing tank and the fermentation tanks; the belt filter press is connected with the fermentation tank; the slurry mixing tank, the fermentation tank and the belt filter press are provided with odor collecting pipelines, the building body is further provided with a dosing assembly which is connected with the fermentation tank, and the building body is provided with a tap water assembly connected with the slurry mixing tank.

This invention provides a method for culturing Aspergillus oryzae, a culture kit, a method for producing mycoprotein, and mycoprotein, all of which result in a culture of Aspergillus oryzae that is rich in γ-aminobutyric acid compared to ordinary Aspergillus oryzae, yields a large amount of microbial cells, and improves the mushroom-like flavor of the purified mycoprotein. [Solution] A method for culturing γ-aminobutyric acid-enriched Aspergillus oryzae is provided, characterized by adding glutamic acid and / or its salt and a vitamin B6-containing component to a culture medium for culturing the Aspergillus oryzae. A culture kit for γ-aminobutyric acid-enriched Aspergillus oryzae is also provided, comprising Aspergillus oryzae and a culture medium for culturing the Aspergillus oryzae, wherein the culture medium contains glutamic acid and / or its salt and a vitamin B6-containing component. Furthermore, a γ-aminobutyric acid-enriched mycoprotein and a method for producing the same are also provided, characterized by having a γ-aminobutyric acid content of 400 mg or more per 100 g dry weight of mycoprotein.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai EPI7 protein, a coding gene and application thereof, the amino acid sequence of the glomus huanghuai EPI7 protein is shown as SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene for coding the EPI7 protein is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai EPI7 protein. The invention proves that the glomus huanghuai EPI7 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI7 protein gene and transient expression of tobacco.

A bioreactor (10) for producing a protein, such as a mycoprotein and / or mycelium fibers, is proposed. The bioreactor (10) comprises a reactor body (12) having a reactor wall (14), the reactor wall (14) confining a reactor volume for incubating an inoculum for producing the protein, the reactor wall (14) having a tapering bottom section (16); a bottom opening (18) provided in the reactor wall (14) at the tapering bottom section (16); and a cap (20) for covering the bottom opening (18), the cap (20) including a top face (50), and a first fluid channel (36) extending through the cap (20), wherein the first fluid channel (36) is configured for transferring fluid between an inside of the reactor body (12) and an outside of the reactor body (12), and wherein an exit opening (46) of the first fluid channel (36) is arranged flush with the top face (50) of the cap (20).

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai CBEL protein, a coding gene and application thereof, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 2. The invention proves that the glomus huanghuai CBEL9 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai CBEL9 protein gene and transient expression of tobacco.

According to the method, firstly, high-proportion bamboo powder is used as a carbon source, and by means of comprehensive regulation and control of bamboo flavones, cellobiose synthesized through fermentation and the like in the bamboo powder, cellulase producing genes and the like of bamboo-decomposing bacteria are induced and activated, so that the bamboo-decomposing bacteria are obtained; meanwhile, the cultivation environment, self-space hybridization, fusion, seed liquid, an enzymolysis agent, a permeation stabilizer, strain enrichment and the like of the bamboo degradation strains are regulated and controlled, and combined bacteria such as bacillus subtilis, aspergillus oryzae, lactic acid bacteria, saccharomycetes, rhizopus and the like are prepared; then the bamboo decomposition combined bacteria synergistically and interactively efficiently degrade nitrogen source bamboo fibers in directional fermentation under substrate optimization and multi-dimensional comprehensive regulation and control conditions, the nitrogen source bamboo fibers are converted and synthesized into high-quality mycoprotein, digestible bamboo protein, soluble sugar and other nutrient substances, the repression effect in the bamboo decomposition fermentation process is eliminated, and the bamboo decomposition effect is improved. The problems of low fermentation strain activity, low adaptability, low efficiency, poor synergy, biological safety, stability and the like are solved.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai effect protein EPI3 protein, and coding and application thereof, the amino acid sequence of the glomus huanghuai effect protein EPI3 protein is shown as SEQ ID NO.2, and the nucleotide sequence for coding the gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai effect protein EPI3 protein. The invention proves that the glomus huanghuai EPI3 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI3 protein gene and transient expression of tobacco.

The invention belongs to the technical field of gene engineering, and discloses a CBEL10 protein of glomus huanghuai, a coding gene and application thereof, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 2. The invention proves that the glomus huanghuai CBEL10 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai CBEL10 protein gene and transient expression of tobacco.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai EPI effect protein as well as a coding gene and application thereof, the glomus huanghuai EPI effect protein is EPI1, the amino acid sequence of the glomus huanghuai EPI effect protein is as shown in SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene for coding the effect protein is as shown in SEQ ID NO.1. The invention proves that the glomus huanghuai EPI1 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI1 protein gene and transient expression of tobacco.

The present invention relates to a dairy substitute comprising a mycoprotein source dispersed in water with high acyl gellan gum. Such dairy substitute is characterised by a texture and flavour close

The invention discloses candida utilis BYC-YP-118 and application thereof, the candida utilis BYC-YP-118 is preserved in China Center for Type Culture Collection, the preservation date is June 23, 2025, and the preservation number is CCTCC NO: M20251460. The content of the mycoprotein is more than or equal to 60% and can reach 67.32% to the maximum, the mycoprotein is rich in valine, lysine, threonine, tryptophan, phenylalanine, methionine, isoleucine, leucine and other essential amino acids for a human body, the nutritional value of the yeast protein is improved, the yeast protein can be used as a high-quality feed additive to effectively replace imported soybean meal and fish meal, the feed cost is reduced, and the economic benefit is increased. Sustainable development of animal husbandry is promoted.

The invention discloses lactobacillus reuteri and application thereof. The lactobacillus reuteri C3 has the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252241. The traditional Chinese medicine composition has the effects of regulating intestinal flora, enhancing immunity, relieving diarrhea, improving allergic symptoms, inhibiting helicobacter pylori and the like. The hermetia illucens larva pulp feed fermented by the lactobacillus reuteri has the advantages of high protein and low antinutritional factors, and can replace soybean meal or fish meal to a certain extent to be used in preparation of livestock and poultry feed. According to the lactobacillus reuteri disclosed by the invention, high-nitrogen wastewater can be efficiently utilized, the wastewater is converted into high-value mycoprotein, and resource utilization of wastes is realized; the wastewater treatment cost is reduced and the circular economy development is promoted.

The invention belongs to the technical field of microorganisms, and particularly relates to pichia kudriavzevii and application thereof. Wherein the pichia kudriavzevii is pichia kudriavzevii AMCC 31367 (pichia kudriavzevii AMCC 31367), and the preservation number of the pichia kudriavzevii AMCC 31367 is CCTCC (China Center for Type Culture Collection) NO: M 20251680. The pichia kudriavzevii has the characteristics of multi-environment tolerance, broad-spectrum applicability of a substrate and high content of protein and amino acid, particularly can resist high-concentration lactic acid, and can still grow under the condition that the concentration of the lactic acid is 170 g / L; the problems of limited raw material selection, low production efficiency, high cost and the like in microbial mycoprotein development are relieved; in addition, the strain has application potential in the fields of biological materials, microbial engineering, environmental governance, feed, food processing and the like.

The invention belongs to the technical field of gene engineering, and discloses a GIP protein for inducing a plant immune function, a coding gene and application thereof, the GIP protein is GIP10, the amino acid sequence of the GIP protein is as shown in SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene of the coding protein is as shown in SEQ ID NO.1. The invention proves that the glomus huanghuai GIP10 protein plays an important role in the process of improving the disease resistance of plants through cloning the glomus huanghuai GIP10 protein gene and transient expression of tobacco.

The invention relates to the technical field of microbial fermentation. The invention aims to provide a method for fermenting duckweed by using a compound probiotic preparation. The method comprises the following steps: mixing duckweed bran, corn flour, brown sugar and inorganic salt to obtain a culture medium; adding a compound probiotic preparation consisting of lactic acid bacteria and saccharomycetes into the culture medium, and uniformly mixing to finish inoculation, so as to obtain an inoculated material; sealing and fermenting the inoculated material to obtain a fermented material; and taking out the fermented material to obtain a feed product. Through the synergistic fermentation effect of lactic acid bacteria and saccharomycetes, duckweed cell walls are decomposed, mycoprotein is synthesized, the true protein content of the final fermented feed is increased, amino acid composition is more balanced, the fermented feed is rich in various digestive enzymes, vitamins and growth factors, and the nutritional value of the fermented feed is remarkably improved. The invention also provides a duckweed fermented product.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai GIP12 protein, a coding gene and application thereof, the amino acid sequence of the glomus huanghuai GIP12 effect protein is shown as SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene for coding the effect protein is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai GIP12 protein. The invention proves that the glomus huanghuai GIP12 protein plays an important role in the process of improving the disease resistance of plants through cloning the glomus huanghuai GIP12 protein gene and transient expression of tobacco.

The invention belongs to the technical field of livestock feed, and discloses a composite boar feed additive and a preparation process thereof. The additive is prepared from tea polyphenol, rosmarinic acid, quercetin, enteric sodium butyrate, yeast cell wall polysaccharide, embedded bacillus subtilis metabolite and a modified starch carrier, the activity of urease and deaminase of posterior intestinal ammonia-producing bacteria is inhibited through multi-component synergism, and nitrogen is regulated and controlled to be converted into mycoprotein. The preparation process comprises the following steps: dissolving and attaching active ingredients, coating enteric-coated pellets, embedding double-layer microcapsules and performing multi-stage mixing. According to the invention, enzyme inhibition functions of tea polyphenol, rosmarinic acid and quercetin are integrated with intestinal barrier strengthening and pH regulation of sodium butyrate, flora regulation of yeast cell wall polysaccharide and targeted bacteriostasis functions of embedded bacillus subtilis metabolites, and accurate release in specific sections of intestinal tracts is realized through enteric coating and microcapsule technologies; on the premise that the protein level of daily ration is not reduced and the reproductive performance is not damaged, the ammonia nitrogen emission is obviously reduced.

The invention relates to the technical field of microorganisms, in particular to a method for preparing microbial protein from soapstock wastewater, and aims to solve the problems of soapstock wastewater pollution and high protein preparation cost. According to the method, soapstock wastewater containing glycerin and sodium acetate is taken as a raw material, and recycling is realized through multi-stage treatment: firstly, toxic impurities are removed through combined detoxification of ferric salt sedimentation filtration, diatomite adsorption and activated carbon adsorption, a nitrogen source, a phosphorus source and growth promoting factors are supplemented after pH is adjusted to construct a balanced nutrition system, and a microbial culture medium is prepared after sterilization; microbial strains are cultured and activated through a liquid seed solution and inoculated into a culture medium for aerated fermentation; and carrying out solid-liquid separation on the fermentation liquor and collecting thalli to obtain microbial mycoprotein, and recycling the separated fermentation waste liquor for nutrition blending. The method realizes unification of efficient purification of the nigre wastewater and preparation of the microbial protein, is environment-friendly in process, low in cost and high in product purity, and has remarkable economic and environmental benefits.

The utility model relates to the technical field of treatment of thallus mash, in particular to a treatment system of thallus protein mash. The treatment system comprises a first vacuum concentration system which comprises a mycoprotein mash buffer tank, a first vacuum concentration device and a concentrated protein mash buffer tank, the mycoprotein mash buffer tank is communicated with the first vacuum concentration device, and the first vacuum concentration device is communicated with the concentrated protein mash buffer tank; the concentrated protein mash buffer tank is communicated with the high-pressure homogenizer; the enzymolysis system comprises a batching tank, an alkali liquor feeding pipe and an enzymolysis tank, the batching tank is communicated with the high-pressure homogenizer, the batching tank is communicated with the enzymolysis tank, and the alkali liquor feeding pipe is communicated with the enzymolysis tank; the purification treatment part is communicated with the enzymolysis tank. According to the treatment system, the first vacuum concentration system 1, the high-pressure homogenizer 2, the enzymolysis system 3 and other operation units are combined, so that the content of peptide substances in the mycoprotein mash can be effectively increased.