57 results about "Mycoprotein" patented technology

The invention relates to the technical field of treatment of thallus mash, in particular to a treatment method and system of thallus protein mash and application of the treatment method and system. The treatment method comprises the following steps: carrying out vacuum concentration on mycoprotein mash to obtain concentrated protein mash; carrying out wall breaking treatment on the concentrated protein mash to obtain concentrated protein mash containing thallus fragments; carrying out neutralization reaction on the concentrated protein mash by using an alkaline solution to obtain a concentrated protein reaction solution; sequentially carrying out enzymolysis reaction and enzyme deactivation treatment on the concentrated protein reaction solution by using a compound enzyme system to obtain a proteolysis reaction solution; wherein the compound enzyme system comprises the following raw materials: lysozyme, alkaline protease, nuclease and flavourzyme; and carrying out purification treatment on the proteolysis reaction liquid to obtain the mycoprotein treatment liquid. According to the treatment method, operations such as vacuum treatment, wall breaking treatment, neutralization reaction treatment and enzymolysis reaction are combined, and the content of peptide substances in the mycoprotein mash is effectively increased to 25% or above.

The utility model provides a mycoprotein production system which is characterized by comprising a building body on which a steel frame platform is built; the slurry mixing tank is fixed on the building body; the plurality of fermentation tanks are fixed on the building body, and a second conveying pipe is arranged between the slurry mixing tank and the fermentation tanks; the belt filter press is connected with the fermentation tank; the slurry mixing tank, the fermentation tank and the belt filter press are provided with odor collecting pipelines, the building body is further provided with a dosing assembly which is connected with the fermentation tank, and the building body is provided with a tap water assembly connected with the slurry mixing tank.

The invention relates to the technical field of microbial fermentation, and discloses a high-protein pig feed prepared by a microbial fermentation technology, which is prepared by the following steps: S1, mixing soybean meal, corn straw and bran according to a mass ratio of 5: 3: 2, crushing to 60-80 meshes, adding a compound microbial agent and water, and adjusting the water content to 45-50% to prepare a fermentation base material; the method comprises the following steps: carrying out first-stage solid-state fermentation for 48-60 hours under the condition of 35-38 DEG C, introducing sterile air in the fermentation process and maintaining the pressure in a tank to be 0.05-0.1 MPa and the stirring speed to be 30-50 rpm, and by arranging a complex microbial inoculant synergistic fermentation system, when the high-protein feed is prepared from soybean meal, corn straws and other plant raw materials, the high-protein feed can be prepared from the soybean meal, the corn straws and other plant raw materials. The specific compatibility proportion of bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae is screened, the synchronous effect of cellulose degradation, organic acid synthesis and mycoprotein enrichment is achieved, and efficient passivation of anti-nutritional factors of different raw materials is guaranteed.

This invention provides a method for culturing Aspergillus oryzae, a culture kit, a method for producing mycoprotein, and mycoprotein, all of which result in a culture of Aspergillus oryzae that is rich in γ-aminobutyric acid compared to ordinary Aspergillus oryzae, yields a large amount of microbial cells, and improves the mushroom-like flavor of the purified mycoprotein. [Solution] A method for culturing γ-aminobutyric acid-enriched Aspergillus oryzae is provided, characterized by adding glutamic acid and / or its salt and a vitamin B6-containing component to a culture medium for culturing the Aspergillus oryzae. A culture kit for γ-aminobutyric acid-enriched Aspergillus oryzae is also provided, comprising Aspergillus oryzae and a culture medium for culturing the Aspergillus oryzae, wherein the culture medium contains glutamic acid and / or its salt and a vitamin B6-containing component. Furthermore, a γ-aminobutyric acid-enriched mycoprotein and a method for producing the same are also provided, characterized by having a γ-aminobutyric acid content of 400 mg or more per 100 g dry weight of mycoprotein.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai EPI7 protein, a coding gene and application thereof, the amino acid sequence of the glomus huanghuai EPI7 protein is shown as SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene for coding the EPI7 protein is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai EPI7 protein. The invention proves that the glomus huanghuai EPI7 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI7 protein gene and transient expression of tobacco.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of gastrointestinal cancer crowds. The culture medium contains yeast extract, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, dietary fiber, porridge, vegetable juice, fruit juice, oat powder, coix seed powder, black bean powder, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of gastrointestinal cancer people, the real intestinal environment of domestic gastrointestinal cancer people is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of the gastrointestinal cancer people can be constructed. The method is beneficial to accurate diagnosis and personalized treatment of intestinal microecology of people with gastrointestinal cancer, and better evaluates the health state of people with gastrointestinal cancer and formulates preventive measures.

The present invention relates to a non-dairy frozen confection comprising mycoprotein biomass in an amount of 0.1 to 2.5 wt%, wherein the mycoprotein biomass comprises mycoprotein fibrils having a fibril length of less than 50 µm.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of people with hyperuricemia. The culture medium contains yeast extract, ethanol, adenine, tryptone, mucoprotein, mycoprotein, insect protein, soybean protein, fructose, glucose, inulin, resistant dextrin, dietary fiber, oxalic acid, saturated fatty acid, sialic acid, peanut oil, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt, cysteine hydrochloride and a plant extract mixture. Vitamin mixed liquid and the like. By means of the bionic colon reactor, by referring to real in-vivo parameters of people suffering from hyperuricemia, the real intestinal environment of people suffering from hyperuricemia in China is well simulated in vitro, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of people suffering from hyperuricemia can be constructed. The method is beneficial to accurate diagnosis of intestinal microecology of people with hyperuricemia, individualized treatment of antisense booking and disease mechanisms, better maintenance of intestinal health and formulation of preventive measures.

A bioreactor (10) for producing a protein, such as a mycoprotein and / or mycelium fibers, is proposed. The bioreactor (10) comprises a reactor body (12) having a reactor wall (14), the reactor wall (14) confining a reactor volume for incubating an inoculum for producing the protein, the reactor wall (14) having a tapering bottom section (16); a bottom opening (18) provided in the reactor wall (14) at the tapering bottom section (16); and a cap (20) for covering the bottom opening (18), the cap (20) including a top face (50), and a first fluid channel (36) extending through the cap (20), wherein the first fluid channel (36) is configured for transferring fluid between an inside of the reactor body (12) and an outside of the reactor body (12), and wherein an exit opening (46) of the first fluid channel (36) is arranged flush with the top face (50) of the cap (20).

The invention relates to a proteolysis product with gastric mucosa repair activity and application thereof. The protein zymolyte with gastric mucosa repair activity is prepared by the following method: firstly, carrying out primary enzymolysis on mycoprotein by using a first enzyme to obtain a primary enzymolysis solution, and then carrying out secondary enzymolysis on the primary enzymolysis solution by using a second enzyme to obtain a secondary enzymolysis solution, the secondary enzymatic hydrolysate is a solution in which the protein hydrolysate with gastric mucosa repair activity is dispersed; the first enzyme comprises aspergillus oryzae protease; the second enzyme comprises aspergillus niger lipase. The proteolysis product provided by the invention has the effects of increasing the antioxidant level of gastric tissues, improving the defense factor level of gastric mucosa and reducing the level of related inflammatory factors in serum, can play a role in protecting the gastric mucosa, has gastric mucosa repairing activity, and is safe and easily available in raw materials, simple in preparation method and easy for batch production.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai CBEL protein, a coding gene and application thereof, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 2. The invention proves that the glomus huanghuai CBEL9 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai CBEL9 protein gene and transient expression of tobacco.

To provide a fermented product of koji mold containing ergothioneine at a high concentration and a method for producing the same.SOLUTION: The present invention provides a method for producing a fermented product of koji mold with high ERG content, by culturing using an ergothioneine high-yield koji mold strain. The resulting high-ERG-content koji mold fermented product contains 50 to 50,000 ppm of ERG and is utilized as a high-ergothioneine-content food material such as mycoprotein or rice koji, or as an intermediate material containing ergothioneine.SELECTED DRAWING: None

The invention relates to a proteolysis product with a gastric mucosa protection effect, a micromolecular proteolysis product and application. The present invention relates to a proteolysis product having a gastric mucosa protective effect, which is obtained by performing enzymolysis on a mycoprotein dispersion liquid using papain to obtain a solution in which the proteolysis product having the gastric mucosa protective effect is dispersed. The proteolysis product provided by the invention has a gastric mucosa protection effect, and especially has obvious protection and repair effects on ethanol-induced gastric mucosa injury; in addition, the raw materials are easy to obtain, safe and reliable, the preparation method is simple, no harmful substance is introduced, and batch production is easy. The proteolysis product provided by the invention has homology of medicine and food, also has a gastric mucosa protection effect, and can be used as a health-care product with an auxiliary protection function on gastric mucosa.

The invention relates to a method and a device for preparing complete cattle feed from double-cropping biological sugarcane. Comprising the following steps: S10, feeding first biological sugarcane into a five-roller squeezer for squeezing, using a small part of squeezed sugarcane juice for preparing lactic acid bacteria acidic fermentation liquor, and using the rest sugarcane juice for preparing syrup; s20, crushing the squeezed bagasse, feeding the crushed bagasse into a reaction tank, and carrying out an ammonia leaching-steam explosion pretreatment process; s30, a compound microbial agent, the pretreated bagasse and the crushed sugarcane leaves are fed into a bioreactor to be fermented for 15-20 days, and the bagasse and the sugarcane leaves rich in mycoprotein and various nutrient substances are obtained; and S40, mixing the fermented bagasse and sugarcane leaves with a compound trace element nutritional agent, a compound vitamin nutritional agent, oil bran, protein mulberry, table salt, corn, sodium bicarbonate, seaweed enzymatic hydrolysate and molasses to obtain the complete cattle feed. The problems that feed prepared from bagasse is poor in palatability and unbalanced in nutritional ingredients, and protein feed is high in cost can be solved.

The invention belongs to the field of microorganisms, and discloses a freshwater aquaculture biological flocculant producing strain and application thereof.The biological flocculant producing strain T-49 can assimilate inorganic nitrogen and convert nitrogen-containing compounds such as ammonia nitrogen in a water body into mycoprotein to form biological floccules capable of being eaten by cultured objects. The bioflocculant producing strain can be prepared into a biological agent or added into fish feed to reduce ammonia nitrogen in water and reduce feed cost. The strain is obtained through screening in a YPG liquid culture medium and shows outstanding water waste flocculation capacity and water ammonia nitrogen removal capacity in flocculation activity determination and ammonia nitrogen removal determination of the strain, and the culture water environment meets the growth condition of T-49. After the T-49 is added, the ammonia nitrogen content of the water body is reduced by 59.60%.

The invention relates to a stirring device for a microbial mycoprotein high-density fermentation system and application, and belongs to the technical field of microbial fermentation. Different from conventional double-layer paddle radial flow stirring in a traditional microbial reactor, the spiral ribbon stirring paddle is designed on the basis of the inclined blade paddle, axial flow can be provided on the basis of radial flow, a uniform three-dimensional mixed flow field is formed, meanwhile, three-dimensional distributed turbulent kinetic energy formed in a bottom dead zone by matching with a bent connecting body can be achieved, and the stirring efficiency is improved. Radial and axial mixing kinetic energy is provided for a bottom dead zone, so that the mixing effect of a mixing flow field is further improved, the overall mixing performance of a bioreactor system is improved, the formation of a mixing dead zone at the bottom of a fermentation tank in a high-density fermentation process is avoided, and thalli are prevented from sinking to the bottom of the tank body; and the bottlenecks of non-uniform material mixing, non-uniform gas distribution and the like of the traditional Rushton blade in the high-density fermentation process of the mycoprotein are improved.

The invention relates to the technical field of microorganisms, and discloses pichia kudriavzevii FJM18 and an application of the pichia kudriavzevii FJM18 in a mycoprotein feed, and the preservation number of the pichia kudriavzevii FJM18 is CGMCC (China General Microbiological Culture Collection Center) No.30405. The FJM18 yeast can effectively convert a substrate into feed rich in nutrition, the nutritional value of the feed is improved, meanwhile, emission of organic pollutants is reduced, and environmental protection is facilitated. Besides, the application of the FJM18 yeast in preparation of a biological feed additive and a biological feed can also improve the biological activity of the feed, enhance the immunity of the poultry and promote the healthy growth of the poultry.

The invention relates to the technical field of microorganisms, in particular to Kazakstein yeast with high protein yield and application of the Kazakstein yeast. The invention provides Kazachstania humii CMRC 12Y, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Kazachstania humii CMRC 12Y is CGMCC No.34656. The Kazachstania humii CMRC 12Y can be used for preparing the Kazachstania humii CMRC 12Y. The bacterial strain is separated from traditional fermented food, has the characteristics of good safety, fast growth, high mycoprotein content and the like, and can realize high biomass growth and high protein expression, and the mycoprotein produced by the bacterial strain has good sensory flavor, can be used for preparing microbial protein related products, and has important application prospects.

The invention belongs to the technical field of biology, and particularly relates to a preparation process for producing aspergillus niger mycoprotein feed by fermenting steam-exploded wheat straws. According to the invention, magnetic silicon dioxide particles are used as a carrier to load a quercetin-polyethylene glycol conjugate, SiO2 (at) QPEG is synthesized and is applied to a preparation process for producing aspergillus niger mycoprotein feed through steam explosion wheat straw fermentation, and the magnetic silicon dioxide particles not only improve the primary fermentation efficiency, but also realize the 10-time cycle attenuation rate lt; 5%. The obtained SiO2 (at) QPEG is applied to a preparation process for producing aspergillus niger mycoprotein feed by fermenting steam-exploded wheat straws, has the characteristics of high aspergillus niger mycoprotein yield and recycling, and highlights the breakthrough of the method in recoverability and economical efficiency.

The invention relates to the technical field of treatment of clostridium mycoprotein, in particular to a method and system for removing unpleasant odor of clostridium mycoprotein. The clostridium mycoprotein is derived from clostridium mash, the clostridium mash comprises ash and organic acid capable of generating unpleasant odor, and the method comprises the following steps: carrying out neutralization reaction on an alkaline solution and the clostridium mash to remove the organic acid so as to obtain a weakly alkaline clostridium reaction solution containing organic acid radical ions; carrying out membrane separation on the clostridium reaction liquid to remove the organic acid radical ions and the ash content so as to obtain a clostridium mycoprotein mixed liquid; and drying the clostridium mycoprotein mixed solution to obtain the clostridium mycoprotein. According to the method, through the steps of neutralization reaction, pH value regulation and control, membrane separation and the like, the bad smell of the clostridium mycoprotein is effectively removed, meanwhile, the normal function of the mycoprotein is guaranteed, and powerful support is provided for further application of the clostridium mycoprotein.

The invention discloses a method for improving the yield of glutamic acid by using an annular process formed by glutamic acid isoelectric mother liquor, and belongs to the technical field of industrial fermentation. The method comprises the following steps: carrying out membrane treatment, two-stage electrodialysis treatment, acid hydrolysis treatment, acid decoloration treatment and other technical means on the glutamic acid isoelectric mother liquor, and finally recycling the obtained hydrolysis decoloration solution to replace sulfuric acid to adjust the isoelectric crystallization operation of the fermentation liquor, thereby constructing an annular process. In the whole annular process, pyroglutamic acid in the isoelectric mother liquor is hydrolyzed into glutamic acid through acid hydrolysis, and the pyroglutamic acid and the glutamic acid in the isoelectric mother liquor are recycled and reused in the operation process of glutamic acid isoelectric crystallization, so that the yield of the glutamic acid is increased; simultaneously generated concentrated solution and concentrated water are respectively filtered by a plate frame, concentrated and dried to obtain mycoprotein and ammonium sulfate. By means of the treatment method, the yield of glutamic acid is increased, the yield of mycoprotein and the yield of ammonium sulfate are increased, and huge economic benefits and environmental benefits are achieved.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of infants. The culture medium contains a yeast extract, tryptone, mucoprotein, mycoprotein, worm protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, breast milk oligosaccharide, dietary fiber, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate, bile salt, a plant extract mixture, a vitamin mixed solution and the like. By means of the bionic colon reactor, the real intestinal environment of domestic infants is well simulated in vitro by referring to real parameters in the infants, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of the infants can be constructed. Accurate diagnosis and personalized treatment of the intestinal microecology of the infants are facilitated, the health state of the infants can be better evaluated, and preventive measures can be better formulated.

According to the method, firstly, high-proportion bamboo powder is used as a carbon source, and by means of comprehensive regulation and control of bamboo flavones, cellobiose synthesized through fermentation and the like in the bamboo powder, cellulase producing genes and the like of bamboo-decomposing bacteria are induced and activated, so that the bamboo-decomposing bacteria are obtained; meanwhile, the cultivation environment, self-space hybridization, fusion, seed liquid, an enzymolysis agent, a permeation stabilizer, strain enrichment and the like of the bamboo degradation strains are regulated and controlled, and combined bacteria such as bacillus subtilis, aspergillus oryzae, lactic acid bacteria, saccharomycetes, rhizopus and the like are prepared; then the bamboo decomposition combined bacteria synergistically and interactively efficiently degrade nitrogen source bamboo fibers in directional fermentation under substrate optimization and multi-dimensional comprehensive regulation and control conditions, the nitrogen source bamboo fibers are converted and synthesized into high-quality mycoprotein, digestible bamboo protein, soluble sugar and other nutrient substances, the repression effect in the bamboo decomposition fermentation process is eliminated, and the bamboo decomposition effect is improved. The problems of low fermentation strain activity, low adaptability, low efficiency, poor synergy, biological safety, stability and the like are solved.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of middle-aged and elderly people. The culture medium contains yeast extract, tryptone, mucoprotein, mycoprotein, worm protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, inulin, resistant dextrin, dietary fiber, sorghum, buckwheat, oat, coix seed, corn germ oil, olive oil, linseed oil, walnut oil, sialic acid, algal oil DHA, potassium chloride, sodium chloride, monopotassium phosphate, sodium sulfate and bile salt. A plant extract mixture, a vitamin mixed solution and the like. By means of the bionic colon reactor, the real intestinal environment of the middle-aged and elderly people in China is well simulated in vitro by referring to real in-vivo parameters of the middle-aged and elderly people, the intestinal flora ecology and intestinal gas distribution of the middle-aged and elderly people are reproduced, and a database of normal intestinal microorganisms and intestinal gas of the middle-aged and elderly people can be constructed; accurate diagnosis and personalized treatment of intestinal microecology of the middle-aged and elderly people are facilitated, and the health state of the middle-aged and elderly people can be better evaluated and preventive measures can be better formulated.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai effect protein EPI3 protein, and coding and application thereof, the amino acid sequence of the glomus huanghuai effect protein EPI3 protein is shown as SEQ ID NO.2, and the nucleotide sequence for coding the gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai effect protein EPI3 protein. The invention proves that the glomus huanghuai EPI3 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI3 protein gene and transient expression of tobacco.

The invention belongs to the technical field of gene engineering, and discloses a CBEL10 protein of glomus huanghuai, a coding gene and application thereof, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 2. The invention proves that the glomus huanghuai CBEL10 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai CBEL10 protein gene and transient expression of tobacco.

The invention discloses a treatment method of methylobacterium single-cell protein fermentation liquor. The treatment method comprises the following steps: carrying out heat preservation on the fermentation liquor for producing single-cell protein thalli at 62-68 DEG C for 100-120 minutes; ultrasonic intermittent treatment is conducted in the last 10-20 minutes of the heat preservation process, the ultrasonic frequency is 18-20 Hz, and the power is 100-120 W; cooling to 35-45 DEG C, and keeping the pH value at 6.0-7.5; carrying out enzymolysis for 3-5 hours by using neutral protease and nuclease; centrifugally separating the fermentation supernatant and mycoprotein; adding chitosan, humic acid and trace elements into the supernate, and uniformly mixing to obtain a pretreatment solution; the invention further discloses a water fertilizing agent prepared from the pretreatment liquid. According to the treatment method of the methylobacterium single-cell protein fermentation liquor, the comprehensive utilization rate of the methylobacterium single-cell protein fermentation liquor is increased when the single-cell protein is produced and the water fertilizing agent is prepared. The produced fertilizer water agent is high in content of organic matters such as amino acid and long in shelf life.

An acid-resistant Cyberlindnera jadinii strain and the use thereof, belonging to the technical field of microorganisms. The Cyberlindnera jadinii strain is Cyberlindnera jadinii C-12-2 and is preserved in Guangdong Microbial Culture Collection Center on March 05, 2024 and assigned the accession number GDMCC NO: 64388. The Cyberlindnera jadinii strain can grow in mediums having a pH value of 3.5, and can achieve a crude protein content of 70.02% after fermentation for 24 h. The Cyberlindnera jadinii strain is obtained by first undergoing heavy ion irradiation mutagenesis and then acid resistance domestication. A test illustrates that the strain can grow in acidic mediums having a pH value of 3.5, overcoming the obstacle of an original strain failing to grow under low pH values. The Cyberlindnera jadinii strain has higher acid resistance and protein production capabilities, and thus can be provided as a high-quality strain resource for mixed bacterial fermentation for producing mycoproteins, mycoprotein feeds or yeast cultures, etc.

The invention discloses a culture medium for colonization of intestinal flora and intestinal gas of obesity people, and belongs to the technical field of biology. The culture medium contains yeast extract, tryptone, mucoprotein, mycoprotein, worm protein, soybean protein, fructo-oligosaccharide, galactooligosaccharide, seed polysaccharide, sorghum, buckwheat, oat, coix seed powder, wheat flour, corn flour, soybean flour, inulin and the like. By means of the bionic colon reactor, the real intestinal environment of obesity people in China is well simulated in vitro by referring to real in-vivo parameters of obesity people, the intestinal flora ecology and intestinal gas distribution are reproduced, and a normal intestinal microorganism and intestinal gas database of obesity people can be constructed. The method is helpful for accurate diagnosis and personalized treatment of intestinal microecology of obesity people, and better evaluates health status of obesity people and formulates preventive measures.

The invention belongs to the technical field of biology, and particularly relates to a technology for producing a mycoprotein feed through multi-strain synergistic fermentation and steam explosion of corn straw. According to the method, aspergillus niger GRJC003 with the preservation number of CCTCC NO: M 2025084 and trichoderma reesei are mixed and fermented, and in combination with optimized conditions such as steam explosion pretreatment and addition of pH response type sophorolipid sustained-release particles, a remarkable effect is achieved in preparation of the mycoprotein feed from the steam-exploded corn straw. According to the process, cellulose is thoroughly hydrolyzed and the tolerance to an inhibitor is enhanced by virtue of a unique enzyme complementation, metabolism reciprocity and microenvironment regulation and control synergistic mechanism of the double strains, so that the yield of mycoprotein is increased, meanwhile, efficient utilization of carbon and nitrogen resources and stable control of the process are realized, and the process has outstanding industrial application value and sustainability.