413 results about "Mycoprotein" patented technology

The invention relates to a special micro-fluidic chip for cholera diagnosis with one-dimensional self-assembly magnetic bead chain electrodes, belonging to the field of testing. Rapid diagnosis of fulminating infectious disease cholera with low cost is one of many expectations in the development of medical technology, and the invention provides a micro-fluidic chip for simultaneously detecting and rapidly diagnosing cholera by using multiple characteristic antibodies of cholera. The chip is provided with three micro liquid storage tanks, and the invention is characterized in that capillary passages in parallel configuration contain in the chip, the parallel configuration contains four micro-passages that are mutually in parallel, four magnetic bead chain working electrodes are respectively installed inside the four micro-passages, the surface substances of the four magnetic bead chain working electrodes are respectively four antibody substances that are cholera TP0821 antibody, cholera TP0319 antibody, cholera TP0624 antibody and cholera O139 mycoprotein antibody. The integrated structure and the appearances of specific magnetic bead chain working electrodes facilitate to improve the diagnosis efficiency of cholera diseases.

The invention relates to a pest-proof disease-resistant organic fertilizer and a preparation method thereof, belonging to the technical field of organic fertilizers. Tobacco waste and oil tea pulp are utilized as main raw materials to develop a method of the pest-proof disease-resistant organic fertilizer. The invention breaks through the limitations of harmful substances and single product effect due to addition of chemical materials in the traditional organic fertilizer. The pest-proof disease-resistant organic fertilizer is prepared from the following raw materials: tobacco waste, oil tea pulp, organic substances, a poising agent and a microbial inoculant. The preparation method of the organic fertilizer comprises the following steps: crushing tobacco straws, tobacco stems, tobacco powder, oil tea pulp and organic substances, spraying the poising agent and microbial inoculant, mixing, carrying out pool stack fermentation and strip stack fermentation, screening and granulating to obtain the insect-proof disease-resistant organic fertilizer. The high-efficiency biological insect-inhibiting factors contained in the organic substances containing massive mycoprotein can effectively inhibit and kill pests and pathogenic bacteria, and solve the problem of environmental pollution caused by the pesticide fertilizer.

The invention belongs to the field of fermented waste liquid treatment in the biological fermentation industry and discloses a fertilizer prepared by utilizing threonine fermented wastes. The fertilizer is prepared by the following steps: (1) preparing threonine fermentation liquor; (2) filtering and discoloring; (3) separating crystals and a mother solution; (4) hydrolyzing mycoprotein; (5) preparing feed liquid A; and (6) preparing the fertilizer. According to the fertilizer, amino acid fermented wastes are effectively utilized, the production cost of the fertilizer is reduced, the wastes are changed into valuable materials, the fertilizer efficiency is lasting, and the fertilizer is superior to like products in the market.

The invention is applied to the production of eucalyptus crops, can improve soil structure and provide sufficient water, oxygen and nutrient to improve the output and the quality of planted eucalyptus crops. The invention relates to a fertilizer containing microorganisms and organic and inorganic substances. The fertilizer adopts cooperation of biotechnology and electrolytic technology, takes the microorganisms and mineralized matter as additives, and is added with nutrient formulation (nitrogen, phosphorus, potassium, sulfur, magnesium, calcium, silicon, boron, zinc, ferrum, molybdenum, and mycoprotein, amino acid, humus, plant nucleic acid, B vitamins, organized enzymes and unidentified factors improving the growth of the crops), which not only can provide sufficient nutrient for the crops, but also improves the soil, and is favorable for planting eucalyptus. Through the biotechnology, the microorganisms in the state of dormancy are injected the fertilizer, and are activated in favorable environment to decompose animal and plant residue, farm chemical, fertilizers, heavy metal toxic residue and minerals in the soil and change the contents of nitrogen, phosphorus and potassium; through the electrolytic technology, mineralized matter additives make the soil carry out chain reaction with the water and the supplied oxygen, so as to gradually change electric charge of ions in the soil, increase oxygen supply to the soil, improve the infiltrability of the water and strengthen conditions of microorganism activities.

The invention relates to a method for preparing an algal biological feed. The method comprises the following steps of: preparing complex microbial inoculants from actinomycetes, saccharomycetes, bacillus subtilis, lactobacillus casei and lactobacillus; and fertilizing algas, and under the synergistic effect of a plurality of strains, generating a mass of cellulase type degraded fibers while synthetising mycoprotein by taking full advantage of an inorganic nitrogen source, an organic nitrogen source and saccharides generated through degradation of the fibers. The algal fermented biological feed provided by the invention has more essential amino-acids and higher nutritive value as compared with algas; animals can absorb the feed quickly and the daily gain can be increased by 4-7%; besides, the immunity of the animals is enhanced, the serum alkaline phosphatase activity is improved by 15% and the alanine transaminase activity is increased by 6%; and the breeding effect is quite obvious. In addition, the preparation method provided by the invention has the advantages of low production cost and short period in contrast with the prior art.

The invention relates to a method for preparing a microbial fertilizer, a nutrient solution, a fodder and a pesticide and for fermentation bed cultivation by utilizing indigenous microorganism probiotics. The microorganism probiotics are formed by fermenting eight pure natural materials, including indigenous bacteria, green juice, fresh fish amino acid, enzyme alcohol, Chinese prescription nutrient solution, lactic acid bacteria, natural calcium and water-soluble calcium phosphate potassium; the microorganism probiotics contain the mixed flock of various beneficial bacteria, such as, lactic acid bacteria, photosynthetic bacteria, bacillus and yeast; the fertilizer can be used for fertilizing and loosening soil; the agricultural products are free from residue, taste nice and are high in yield; the fodder can be used for guaranteeing no residue on the cultured animal meat product; the meat is tender and free from smell; after the microorganism nutrient solution is used, the microorganism pesticide can prevent and treat insect diseases; the chemical pesticide and the fertilizer residue can be eliminated and decomposed; when the fermentation bed is used for culturing, the excrement cleaning is not required; the animal excrement is decomposed by the fermented padding while beneficial mycoprotein is formed; the fodder can be eaten by the animals; the microbial fertilizer is odorless, zero-emission, environment-friendly, pollution-free to environment and beneficial to the health of human and animals.

The invention discloses an amino acid fermentation bacteria utilization method, which belongs to the technical field of amino acid production. The method comprises the steps that by taking glutamic acid fermentation as an example and adopting the isoelectric point of fermentation broth, a disc separator is utilized to separate mycoprotein in the fermentation broth, and a moderate amount of compound enzyme is added into the mycoprotein serum, so that the walls of bacteria are broken and the bacteria are enzymetically hydrolyzed. The enzymatic hydrolysate is separated by the high-speed disc separator, and the cell walls are removed, the obtained clarified enzymatic hydrolysate is concentrated under low temperature to produce the enzymetically hydrolyzed mycoprotein extract, or the clarified enzymatic hydrolysate is dried by a gunite granulation fluidized bed drier, a spray granulation drier and the like to produce the enzymetically hydrolyzed mycoprotein powder. In the method, the utilization value of the mycoprotein is greatly increased, so that the mycoprotein is changed from cheap feed into high value-added yeast extract and yeast powder substitute, the product can be used as fermentation ingredient and in other fields, the resources can be sufficiently utilized in the whole process, the purposes of energy saving and consumption reduction are achieved, and the invention has a broad application prospect.

The invention discloses cassava straw silage and a preparation method thereof. The silage is prepared from, by weight, 150-200 parts of cassava straw, 30-35 parts of sunflower stems and leaves, 10-20 parts of sweet potato vine, 30-40 parts of corn straw, 30-50 parts of banana leaves and 10-15 parts of sticktight, and straw leavening agents are added according to the proportion of 11g/kg of the total mass of the mentioned raw materials. According to the cassava straw silage and the preparation method thereof, the cassava straw serves as the main raw material, the sunflower stems and leaves, the sweet potato vine, the corn straw, the banana leaves and the sticktight serve as auxiliary materials, the straw leavening agents are used for fermenting the raw materials, and a great amount of microorganism mycoprotein and beneficial metabolite are generated and accumulated. What's more important, the effect of microorganism culture in the straw leavening agents is utilized, cellulose and lignin in the cassava straw and the corn straw are degraded, meanwhile, the sweet potato vine, the banana leaves and the sunflower stems and leaves are added, all the constituents work together to improve palatability of the cassava straw, improve digestibility and enrich nutrient compositions of the silage, and the silage can be stored for a long time without mildewing or rotting.

The invention relates to a method for purifying and preparing a recombinant anti-VEGF antibody fragment which is periplasmically-expressed in escherichia coli. The key technique is the purification and preparation process and method of an anti-VEGF antibody fragment which is periplasmically-expressed in escherichia coli. When the recombinant antibody is purified, a soluble reinforcing reagent is added in the periplasm extraction process to facilitate the correct folding of the heavy-chain and light-chain of the antibody. Before the antibody fragment is purified, heat treatment is performed to inactivate and deposit a part of host mycoprotein and thus to make the antibody molecule more stable. During the purification process, the two-step purification of cation exchange-hydrophobic chromatography is carried out to produce the antibody protein with purity of more than 95%. The method is easy and convenient to operate, is low in cost and has a high yield, and is suitable for industrialization.

The invention relates to a sweet potato skin/sweet potato powder dreg fermented feed and a preparation method thereof. The fermented feed comprises fresh sweet potato skins or sweet potato dregs, an adsorbent, dreg cakes, urea, ammonium sulfate, calcium hydrophosphate, magnesium sulfate, Aspergillus oryzae, Aspergillus niger, Saccharomyces cerevisiae, Candida, and lactobacillus. By means of the oxygen-consuming fermentation of the Aspergillus niger, saccharomycetes and Aspergillus oryzae, all of which are capable of favorably producing various enzyme systems, pectin, crude fibers, protein, starch and other macromolecular substances in fermentation materials are degraded rapidly; in addition, an inorganic nitrogen source is converted into mycoprotein, wherein the lactobacillus produces lactic acid and other active nutrient substances by using micromolecular nutrient substances. The preparation method has the advantage of reducing environment pollution and resource waste caused by difficulty in utilizing the sweet potato skins and the sweet potato powder dregs; after being fed, the fermented feed has the advantages of reducing cultivation cost, increasing cultivation benefits, reducing the cost investment in enzyme preparations and protein feed and increasing the productivity. The fermented feed is a novel resource-utilization type biological feed and has very high economic and social effects.

The invention relates to a method for producing mycoprotein and polypeptide organic fertilizer by waste fermented mash and gulonic acid mother solution formed in the vitamin C production technology, which belongs to the field of biotechnology and waste resource utilization technology, and comprises the following steps: adding 5 to 20 parts of gulonic acid mother solution in 100 parts of waste vitamin C fermented mash protein, adjusting pH value to 2.0 to 5.0, and hydrolyzing for 0.5 to 12 hours under the temperature of 40 to 70 DEG C. On the one hand part of protein in fermented mash protein is hydrolyzed to become polypeptide and amino acid to dissolve in water, through adjusting pH value and appropriate concentration, right amount of trace element is added, obtaining polypeptide organic fertilizer, on the other hand the rest protein in mash protein is precipitated, and the precipitated protein is made into mycoprotein through filter-pressing, drying and smashing. The invention recycles the vitamin C fermentation industry waste to produce mycoprotein and polypeptide organic fertilizer, thus not only solving the problem of industrial pollution, but also providing organic fertilizer and feed protein for agricultural production.

The invention is applied to saline soil, can improve soil structure and provide sufficient water, oxygen and nutrient to improve the output and the quality of crops. The invention relates to a liquid fertilizer containing microorganisms and rich inorganic nutrient. The fertilizer adopts cooperation of biotechnology and electrolytic technology, takes the microorganisms and mineralized matter as additives, and is added with nutrient formulation (nitrogen, phosphorus, potassium, sulfur, magnesium, calcium, silicon, boron, zinc, ferrum, molybdenum, and mycoprotein, amino acid, humus, plant nucleic acid, B vitamins, organized enzymes and unidentified factors improving the growth of the crops), which not only can provide sufficient nutrient for the crops, but also improves the saline soil, and is favorable for planting the crops. Through the biotechnology, the microorganisms in the state of dormancy are injected the fertilizer, and are activated in favorable environment to decompose animal and plant residue, farm chemical, fertilizers, heavy metal toxic residue and minerals in the soil and change the contents of nitrogen, phosphorus and potassium; through the electrolytic technology, mineralized matter additives make the soil carry out chain reaction with the water and the supplied oxygen, so as to gradually change electric charge of ions in the soil, increase oxygen supply to the soil, improve the infiltrability of the water and strengthen conditions of microorganism activities.

The invention provides a preparation method for a cornstalk-bagasse mixed fermented feed. According to the invention, dried cornstalk and bagasse are used as raw materials; ammoniated treatment is carried out at first so as to remove lignin, hemicellulose and cellulose in cornstalk and bagasse; a mixed fermentation bacteria solution I and a mixed fermentation bacteria solution II are sprayed on the mixed material of cornstalk and bagasse for two-step fermentation so as to fully degrade lignin, hemicellulose and cellulose in the mixed material and increase the content of mycoprotein, thereby increasing the content of crude protein and other nutrients in a final product of the feed. The invention has the following advantages: common bacterial strains for feeds are combined in the method, and the bacterial strains used in the method do not generate an obvious antagonistic effect and are not pathogenic bacterial systems; collective fermentation of the mixed bacterial systems enables the utilization efficiency of cornstalk and bagasse resources and the content of nutrients to be effectively increased; equipment and process flow are simple, convenient to operate and easy to popularize and apply, and the preparation method in the invention provides an effective channel for environmental protection and high efficiency reutilization of resources and promotes the progress of resource utilization technology of crop straws.

The invention relates to a method for preparing chitin and composite protein powder by composite microbial fermentation of prawn leftovers, which comprises the following steps: homogenizing prawn leftovers, adding composite microbe (lactobacillus bulgaricus and bacillus subtilis) according to a certain ratio, fermenting for 48 hours, separating the prawn shells from the fermentation liquid, drying and crushing the prawn shells to obtain chitin, adjusting the pH of the fermentation liquid, adding powdery soybean meal, inoculating the bacillus subtilis, continuing to perform solid fermentation for 48 hours, drying after fermentation to prepare composite protein powder. The product prepared in the invention contains both microbial mycoprotein and vegetable protein degradation products and animal protein, is rich in nutrition, and has functional activity; The greateat advantage is the green utilization of prawn leftovers, which discharges no pollutant, realizes clean production, and obtains the products of chitin and composite protein powder with high added value.

The invention relates to a panus giganteus culture medium. The panus giganteus culture medium is prepared by the following raw materials in parts by weight: 28-35 parts of broadleaf sawdust, 15-20 parts of wheat bran, 7-14 parts of soybean curd residue, 3-5 parts of monosodium glutamate mycoprotein, 5-10 parts of semen litchi particles, 12-15 parts of pineapple peels, 4-6 parts of silkworm excrement, 13-18 parts of dry poultry manure, 5-8 parts of pine needle meal, 1-2 parts of montmorillonite powder, 1-2 parts of calcined lime, 0.5-1 part of calcium superphosphate, 1-2 parts of kieselguhr and 5-10 parts of nutrition additives. According to the culture medium, sources of main materials are extensive, the cost is low, various nutrient elements are adopted, components are rich; the culture medium is synergetic with the nutrition additives and is easily absorbed by the thallus, so that the biotransformation efficiency is improved, and the nutrition is rich; compared with the normal culture medium, the panus giganteus culture medium has the advantages that the yield of the thallus in per kilogram of the culture medium is increased by 60-75%; the cultured thallus is thick and strong, and strong in stress resistance.

The invention is applied to the production of organic crops, can improve soil structure and provide sufficient water, oxygen and nutrient to improve the output and the quality of planted crops. The invention relates to a fertilizer containing microorganisms and organic matter. The fertilizer adopts cooperation of biotechnology and electrolytic technology, takes the microorganisms and mineralized matter as additives, and is added with nutrient formulation (nitrogen, phosphorus, potassium, sulfur, magnesium, calcium, silicon, boron, zinc, ferrum, molybdenum, and mycoprotein, amino acid, humus, plant nucleic acid, B vitamins, organized enzymes and unidentified factors improving the growth of the crops), which not only can provide sufficient nutrient for the crops, but also improves the soil, and is favorable for planting the organic crops. Through the biotechnology, the microorganisms in the state of dormancy are injected the fertilizer, and are activated in favorable environment to decompose animal and plant residue, farm chemical, fertilizers, heavy metal toxic residue and minerals in the soil and change the contents of nitrogen, phosphorus and potassium; through the electrolytic technology, mineralized matter additives make the soil carry out chain reaction with the water and the supplied oxygen, so as to gradually change electric charge of ions in the soil, increase oxygen supply to the soil, improve the infiltrability of the water and strengthen conditions of microorganism activities.

A food containing mycoprotein and with high nutritive value and delicious taste contains mycoprotein (40-100 Wt. Portions) and flavouring (1-5). It is prepared from edible fungus through drying, roasting and grinding.

The invention discloses a novel process for producing monosodium glutamate, which comprises the following key points: performing two stages of membrane filtrations on a glutamic acid fermentation broth obtained through fermentation so as to eliminate mycoproteins and other macromolecular substances; performing decolorization through active carbons, sheet frame filtration, alkali neutralization and concentration on the fermentation broth; performing ammonia blowoff recycling in the process of the concentration; performing further decolorization through a particle carbon column; and performing concentration and crystallization to prepare the final product of the monosodium glutamate. The process achieves the procedure of converting the glutamic acid fermentation broth into sodium glutamate directly without extracting the glutamic acid, has the advantages of simple operation, short production period, low cost, less pollution, high added value of by-products, and the like, and solves the problems that the production of the monosodium glutamate by the prior art has over-dependence on sulphuric acid, high yield of wastewater and lower quality of the by-products.

The invention provides a method for cleanly producing glutamic acid, gamma-polyglutamic acid and an organic fertilizer, relating to a method for sequentially preparing the glutamic acid, the gamma-polyglutamic acid and an organic slow-release fertilizer with functions of retaining water and humidity by adopting a microbiological fermentation method. According to the method provided by the invention, the gamma-polyglutamic acid is produced by using electric waste liquid such as the glutamic acid; the extraction rate of the glutamic acid is only 70-80 percent; the gamma-polyglutamic acid, a large amount of mycoprotein and the organic slow-release fertilizer with functions of retaining water and humidity, with higher added values, are simultaneously obtained; an extraction mother solution of the glutamic acid is simply adjusted to be used as a culture medium for producing the gamma-polyglutamic acid, so that a hybridization process is omitted and the discharge amount of waste water in the extraction process of the glutamic acid is reduced and zero discharge is basically realized; and the pressure of glutamic acid production enterprises on environment pollution is reduced and the aim of clean production is achieved.

The invention discloses a functional complex microbial agent serving as aquaculture bait, and relates to a microbial agent. The functional complex microbial agent comprises the following ingredients: bacillus, yeast and a photosynthetic bacterium. Compared with the existing agent, the functional complex microbial agent is used for aquaculture of fishes and the like, can convert eutrophicated water into substances, such as beneficial mycoprotein, amino acid, polypeptide, carbohydrate and fatty acid, which are suitable for growth and absorption of plankton required by growth of spotted silver carps, silver carps and the like.

The invention discloses a method for producing erythritol. Glucose is taken as raw material, candidalipolytica is taken as a fermentation strain, shaking culture as well as first-stage and second-stage inoculum development are performed, then the fermentation in a fermentation tank is carried out, mycoprotein impurities in zymotic fluid are removed by a ceramic membrane after the fermentation is finished, then a sodium membrane is used to selectively pass through the erythritol so as to extract the erythritol, and finally the erythritol product is obtained through condensation crystallization and separation drying. The method has the advantages of short incubation period, high extraction yield, low production cost and so on.

The invention discloses a microbial fermentation bed strain and a manufacturing method thereof. The microbial fermentation bed strain comprises the following components in percentage by weight: 50 to 70 percent of bacillus subtilis strain and 30 to 50 percent of saccharomyces cerevisiae. The strain adopted by the invention is safe and reliable; the production is low in cost, energy-saving and environmentally-friendly and adopts simple and controllable process; the pathogenic bacteria, parasite ova, grass seed and the like in packing can be killed; the strain can decompose livestock and poultry manure quickly, optimize breeding environment and convert the livestock and poultry manure into probiotic bacterium mycoproteins used for feeding the livestock and poultry, so the material to meat (egg) ratio is lowered effectively and breeding economic benefit is improved; and in a packing fermentation process, these kinds of bacteria have a mutually beneficial and symbiotic effect on the dynamic change of the pH value and the oxygen content, so a high constant stability is ensured in the long-term operation of the fermentation bed, and the requirement on the packing is low.