31 results about "Mycoprotein" patented technology

The utility model provides a mycoprotein production system which is characterized by comprising a building body on which a steel frame platform is built; the slurry mixing tank is fixed on the building body; the plurality of fermentation tanks are fixed on the building body, and a second conveying pipe is arranged between the slurry mixing tank and the fermentation tanks; the belt filter press is connected with the fermentation tank; the slurry mixing tank, the fermentation tank and the belt filter press are provided with odor collecting pipelines, the building body is further provided with a dosing assembly which is connected with the fermentation tank, and the building body is provided with a tap water assembly connected with the slurry mixing tank.

The invention relates to the technical field of microbial fermentation, and discloses a high-protein pig feed prepared by a microbial fermentation technology, which is prepared by the following steps: S1, mixing soybean meal, corn straw and bran according to a mass ratio of 5: 3: 2, crushing to 60-80 meshes, adding a compound microbial agent and water, and adjusting the water content to 45-50% to prepare a fermentation base material; the method comprises the following steps: carrying out first-stage solid-state fermentation for 48-60 hours under the condition of 35-38 DEG C, introducing sterile air in the fermentation process and maintaining the pressure in a tank to be 0.05-0.1 MPa and the stirring speed to be 30-50 rpm, and by arranging a complex microbial inoculant synergistic fermentation system, when the high-protein feed is prepared from soybean meal, corn straws and other plant raw materials, the high-protein feed can be prepared from the soybean meal, the corn straws and other plant raw materials. The specific compatibility proportion of bacillus licheniformis, lactobacillus plantarum and saccharomyces cerevisiae is screened, the synchronous effect of cellulose degradation, organic acid synthesis and mycoprotein enrichment is achieved, and efficient passivation of anti-nutritional factors of different raw materials is guaranteed.

This invention provides a method for culturing Aspergillus oryzae, a culture kit, a method for producing mycoprotein, and mycoprotein, all of which result in a culture of Aspergillus oryzae that is rich in γ-aminobutyric acid compared to ordinary Aspergillus oryzae, yields a large amount of microbial cells, and improves the mushroom-like flavor of the purified mycoprotein. [Solution] A method for culturing γ-aminobutyric acid-enriched Aspergillus oryzae is provided, characterized by adding glutamic acid and / or its salt and a vitamin B6-containing component to a culture medium for culturing the Aspergillus oryzae. A culture kit for γ-aminobutyric acid-enriched Aspergillus oryzae is also provided, comprising Aspergillus oryzae and a culture medium for culturing the Aspergillus oryzae, wherein the culture medium contains glutamic acid and / or its salt and a vitamin B6-containing component. Furthermore, a γ-aminobutyric acid-enriched mycoprotein and a method for producing the same are also provided, characterized by having a γ-aminobutyric acid content of 400 mg or more per 100 g dry weight of mycoprotein.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai EPI7 protein, a coding gene and application thereof, the amino acid sequence of the glomus huanghuai EPI7 protein is shown as SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene for coding the EPI7 protein is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai EPI7 protein. The invention proves that the glomus huanghuai EPI7 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI7 protein gene and transient expression of tobacco.

A bioreactor (10) for producing a protein, such as a mycoprotein and / or mycelium fibers, is proposed. The bioreactor (10) comprises a reactor body (12) having a reactor wall (14), the reactor wall (14) confining a reactor volume for incubating an inoculum for producing the protein, the reactor wall (14) having a tapering bottom section (16); a bottom opening (18) provided in the reactor wall (14) at the tapering bottom section (16); and a cap (20) for covering the bottom opening (18), the cap (20) including a top face (50), and a first fluid channel (36) extending through the cap (20), wherein the first fluid channel (36) is configured for transferring fluid between an inside of the reactor body (12) and an outside of the reactor body (12), and wherein an exit opening (46) of the first fluid channel (36) is arranged flush with the top face (50) of the cap (20).

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai CBEL protein, a coding gene and application thereof, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 2. The invention proves that the glomus huanghuai CBEL9 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai CBEL9 protein gene and transient expression of tobacco.

The invention belongs to the field of microorganisms, and discloses a freshwater aquaculture biological flocculant producing strain and application thereof.The biological flocculant producing strain T-49 can assimilate inorganic nitrogen and convert nitrogen-containing compounds such as ammonia nitrogen in a water body into mycoprotein to form biological floccules capable of being eaten by cultured objects. The bioflocculant producing strain can be prepared into a biological agent or added into fish feed to reduce ammonia nitrogen in water and reduce feed cost. The strain is obtained through screening in a YPG liquid culture medium and shows outstanding water waste flocculation capacity and water ammonia nitrogen removal capacity in flocculation activity determination and ammonia nitrogen removal determination of the strain, and the culture water environment meets the growth condition of T-49. After the T-49 is added, the ammonia nitrogen content of the water body is reduced by 59.60%.

The invention relates to the technical field of microorganisms, in particular to Kazakstein yeast with high protein yield and application of the Kazakstein yeast. The invention provides Kazachstania humii CMRC 12Y, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the Kazachstania humii CMRC 12Y is CGMCC No.34656. The Kazachstania humii CMRC 12Y can be used for preparing the Kazachstania humii CMRC 12Y. The bacterial strain is separated from traditional fermented food, has the characteristics of good safety, fast growth, high mycoprotein content and the like, and can realize high biomass growth and high protein expression, and the mycoprotein produced by the bacterial strain has good sensory flavor, can be used for preparing microbial protein related products, and has important application prospects.

The invention belongs to the technical field of biology, and particularly relates to a preparation process for producing aspergillus niger mycoprotein feed by fermenting steam-exploded wheat straws. According to the invention, magnetic silicon dioxide particles are used as a carrier to load a quercetin-polyethylene glycol conjugate, SiO2 (at) QPEG is synthesized and is applied to a preparation process for producing aspergillus niger mycoprotein feed through steam explosion wheat straw fermentation, and the magnetic silicon dioxide particles not only improve the primary fermentation efficiency, but also realize the 10-time cycle attenuation rate lt; 5%. The obtained SiO2 (at) QPEG is applied to a preparation process for producing aspergillus niger mycoprotein feed by fermenting steam-exploded wheat straws, has the characteristics of high aspergillus niger mycoprotein yield and recycling, and highlights the breakthrough of the method in recoverability and economical efficiency.

According to the method, firstly, high-proportion bamboo powder is used as a carbon source, and by means of comprehensive regulation and control of bamboo flavones, cellobiose synthesized through fermentation and the like in the bamboo powder, cellulase producing genes and the like of bamboo-decomposing bacteria are induced and activated, so that the bamboo-decomposing bacteria are obtained; meanwhile, the cultivation environment, self-space hybridization, fusion, seed liquid, an enzymolysis agent, a permeation stabilizer, strain enrichment and the like of the bamboo degradation strains are regulated and controlled, and combined bacteria such as bacillus subtilis, aspergillus oryzae, lactic acid bacteria, saccharomycetes, rhizopus and the like are prepared; then the bamboo decomposition combined bacteria synergistically and interactively efficiently degrade nitrogen source bamboo fibers in directional fermentation under substrate optimization and multi-dimensional comprehensive regulation and control conditions, the nitrogen source bamboo fibers are converted and synthesized into high-quality mycoprotein, digestible bamboo protein, soluble sugar and other nutrient substances, the repression effect in the bamboo decomposition fermentation process is eliminated, and the bamboo decomposition effect is improved. The problems of low fermentation strain activity, low adaptability, low efficiency, poor synergy, biological safety, stability and the like are solved.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai effect protein EPI3 protein, and coding and application thereof, the amino acid sequence of the glomus huanghuai effect protein EPI3 protein is shown as SEQ ID NO.2, and the nucleotide sequence for coding the gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai effect protein EPI3 protein. The invention proves that the glomus huanghuai EPI3 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI3 protein gene and transient expression of tobacco.

The invention belongs to the technical field of gene engineering, and discloses a CBEL10 protein of glomus huanghuai, a coding gene and application thereof, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO: 2. The invention proves that the glomus huanghuai CBEL10 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai CBEL10 protein gene and transient expression of tobacco.

An acid-resistant Cyberlindnera jadinii strain and the use thereof, belonging to the technical field of microorganisms. The Cyberlindnera jadinii strain is Cyberlindnera jadinii C-12-2 and is preserved in Guangdong Microbial Culture Collection Center on March 05, 2024 and assigned the accession number GDMCC NO: 64388. The Cyberlindnera jadinii strain can grow in mediums having a pH value of 3.5, and can achieve a crude protein content of 70.02% after fermentation for 24 h. The Cyberlindnera jadinii strain is obtained by first undergoing heavy ion irradiation mutagenesis and then acid resistance domestication. A test illustrates that the strain can grow in acidic mediums having a pH value of 3.5, overcoming the obstacle of an original strain failing to grow under low pH values. The Cyberlindnera jadinii strain has higher acid resistance and protein production capabilities, and thus can be provided as a high-quality strain resource for mixed bacterial fermentation for producing mycoproteins, mycoprotein feeds or yeast cultures, etc.

The invention belongs to the technical field of biology, and particularly relates to a technology for producing a mycoprotein feed through multi-strain synergistic fermentation and steam explosion of corn straw. According to the method, aspergillus niger GRJC003 with the preservation number of CCTCC NO: M 2025084 and trichoderma reesei are mixed and fermented, and in combination with optimized conditions such as steam explosion pretreatment and addition of pH response type sophorolipid sustained-release particles, a remarkable effect is achieved in preparation of the mycoprotein feed from the steam-exploded corn straw. According to the process, cellulose is thoroughly hydrolyzed and the tolerance to an inhibitor is enhanced by virtue of a unique enzyme complementation, metabolism reciprocity and microenvironment regulation and control synergistic mechanism of the double strains, so that the yield of mycoprotein is increased, meanwhile, efficient utilization of carbon and nitrogen resources and stable control of the process are realized, and the process has outstanding industrial application value and sustainability.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai EPI effect protein as well as a coding gene and application thereof, the glomus huanghuai EPI effect protein is EPI1, the amino acid sequence of the glomus huanghuai EPI effect protein is as shown in SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene for coding the effect protein is as shown in SEQ ID NO.1. The invention proves that the glomus huanghuai EPI1 protein plays an important role in the process of improving the disease resistance of plants through cloning of the glomus huanghuai EPI1 protein gene and transient expression of tobacco.

The present invention relates to a dairy substitute comprising a mycoprotein source dispersed in water with high acyl gellan gum. Such dairy substitute is characterised by a texture and flavour close

The invention belongs to the technical field of extraction, and discloses a method, system and application for recovering high-value liquid ammonia and protein from glutamic acid waste mother liquor, and the method comprises the following steps: extracting mycoprotein from glutamic acid waste mother liquor, concentrating, feeding into a reaction tank, adding an ammonia gas decomposing agent into the reaction tank, controlling the pH value, continuously stirring, and carrying out solid-liquid separation; the solution violently reacts and releases dilute ammonia gas, and the ammonia gas is compressed, sterilized and conveyed to be fermented to be used as a nitrogen source; performing solid-liquid separation on the mother liquor to obtain salt solid products for sale, and preparing the separated mother liquor into feed for sale. According to the novel green method for extracting the ammonia gas from the glutamic acid waste mother liquor and efficiently recovering the protein feed, the mother liquor is subjected to component analysis, different methods are adopted for separation according to different components, high-added-value products ammonia and protein are extracted and separated, green recovery is implemented, the added value and the overall value are improved, the environmental protection problem is solved, and the method is worthy of popularization and application. The economic benefit is achieved, and meanwhile the great social value is achieved.

The invention discloses candida utilis BYC-YP-118 and application thereof, the candida utilis BYC-YP-118 is preserved in China Center for Type Culture Collection, the preservation date is June 23, 2025, and the preservation number is CCTCC NO: M20251460. The content of the mycoprotein is more than or equal to 60% and can reach 67.32% to the maximum, the mycoprotein is rich in valine, lysine, threonine, tryptophan, phenylalanine, methionine, isoleucine, leucine and other essential amino acids for a human body, the nutritional value of the yeast protein is improved, the yeast protein can be used as a high-quality feed additive to effectively replace imported soybean meal and fish meal, the feed cost is reduced, and the economic benefit is increased. Sustainable development of animal husbandry is promoted.

The invention discloses lactobacillus reuteri and application thereof. The lactobacillus reuteri C3 has the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252241. The traditional Chinese medicine composition has the effects of regulating intestinal flora, enhancing immunity, relieving diarrhea, improving allergic symptoms, inhibiting helicobacter pylori and the like. The hermetia illucens larva pulp feed fermented by the lactobacillus reuteri has the advantages of high protein and low antinutritional factors, and can replace soybean meal or fish meal to a certain extent to be used in preparation of livestock and poultry feed. According to the lactobacillus reuteri disclosed by the invention, high-nitrogen wastewater can be efficiently utilized, the wastewater is converted into high-value mycoprotein, and resource utilization of wastes is realized; the wastewater treatment cost is reduced and the circular economy development is promoted.

The invention discloses an application method of a three-step nitrogen conservation synergistic compost product in contaminated soil remediation, and belongs to the technical field of organic waste compost fermentation. Aiming at the problems of difficulty in low-temperature starting, long fermentation period, serious greenhouse gas emission and serious nitrogen loss in the existing composting technology, a temperature-control time-sharing inoculation technology is adopted: a low-temperature-resistant lignocellulose degrading bacterium LDT1 is added in a low-temperature starting period to realize quick starting at 10-15 DEG C; a multi-element synergistic emission reduction system composed of zeolite, calcium superphosphate and ferrous sulfate is added in the high-temperature stable period; an alkali-resistant ammonia assimilation strain JS2 is added in the nitrogen retention period, and free ammonia nitrogen is converted into mycoprotein. According to the method, the composting period is shortened from 90-270 days to 30-40 days, the NH3 emission amount is reduced by 70-76%, the N2O emission amount is reduced by 35-45%, the CO2 emission amount is reduced by 45-55%, the ammonia nitrogen content is increased by 3-5 times compared with that of a traditional method, and collaborative optimization of degradation, emission reduction and nitrogen conservation is achieved.

The present invention relates to a plant-based, edible fat-containing product comprising an aqueous phase and 30 - 99 wt.% of a continuous fat phase, further comprising 0.01 - 5.0 wt.% of mycoprotein. Examples of a product are a low-fat spread, high-fat spread and a wrapper. Methods for preparation are also disclosed. The fat-containing product has favourable salt-release properties.

The invention belongs to the technical field of microorganisms, and particularly relates to pichia kudriavzevii and application thereof. Wherein the pichia kudriavzevii is pichia kudriavzevii AMCC 31367 (pichia kudriavzevii AMCC 31367), and the preservation number of the pichia kudriavzevii AMCC 31367 is CCTCC (China Center for Type Culture Collection) NO: M 20251680. The pichia kudriavzevii has the characteristics of multi-environment tolerance, broad-spectrum applicability of a substrate and high content of protein and amino acid, particularly can resist high-concentration lactic acid, and can still grow under the condition that the concentration of the lactic acid is 170 g / L; the problems of limited raw material selection, low production efficiency, high cost and the like in microbial mycoprotein development are relieved; in addition, the strain has application potential in the fields of biological materials, microbial engineering, environmental governance, feed, food processing and the like.

The utility model discloses a treatment assembly for mycoprotein, and the treatment assembly for mycoprotein comprises a first liquid storage tank which is used for storing a bacterial stock solution; the device comprises a first liquid storage tank, a steam ejector and a second liquid storage tank, a mixing space is formed in the steam ejector, the mixing space is provided with a liquid inlet, a steam inlet and a steam outlet, the liquid inlet is used for being communicated with the first liquid storage tank, the steam inlet is used for being communicated with steam in an on-off mode, and the steam outlet is used for being communicated with the second liquid storage tank; and the filtering device is communicated with the second liquid storage tank. According to the treatment assembly for the mycoprotein, the bacterial stock solution can be instantly heated through steam, denaturation of the mycoprotein is achieved, the product quality is guaranteed, the defect that the color of a mycoprotein product is deepened due to long-time heating is overcome, and meanwhile after the mycoprotein is heated and denatured, the separation performance of the mycoprotein is improved.

The invention belongs to the technical field of gene engineering, and discloses a GIP protein for inducing a plant immune function, a coding gene and application thereof, the GIP protein is GIP10, the amino acid sequence of the GIP protein is as shown in SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene of the coding protein is as shown in SEQ ID NO.1. The invention proves that the glomus huanghuai GIP10 protein plays an important role in the process of improving the disease resistance of plants through cloning the glomus huanghuai GIP10 protein gene and transient expression of tobacco.

The present invention relates to a method for preparing a food or feed product comprising mycoprotein. The method comprises the steps a) of providing an alginate premix, that is comprising alginate and an aqueous solution. The method further comprises a step b) of providing mycoprotein and optionally at least one further ingredient, and at least one of b1) and b2), with b1) the mycoprotein being in unfrozen form, and b2) at least 70% of the total alginate content of the mass of step c) is provided in the form of the alginate premix from step a). In a subsequent step c), the alginate premix from step a), the mycoprotein from step b) and optionally the at least one further ingredient from step 2), are mixed and a mass is obtained. In step d) the mass is shaped and cured, and the food or feed product is obtained.

The invention relates to the technical field of microbial fermentation. The invention aims to provide a method for fermenting duckweed by using a compound probiotic preparation. The method comprises the following steps: mixing duckweed bran, corn flour, brown sugar and inorganic salt to obtain a culture medium; adding a compound probiotic preparation consisting of lactic acid bacteria and saccharomycetes into the culture medium, and uniformly mixing to finish inoculation, so as to obtain an inoculated material; sealing and fermenting the inoculated material to obtain a fermented material; and taking out the fermented material to obtain a feed product. Through the synergistic fermentation effect of lactic acid bacteria and saccharomycetes, duckweed cell walls are decomposed, mycoprotein is synthesized, the true protein content of the final fermented feed is increased, amino acid composition is more balanced, the fermented feed is rich in various digestive enzymes, vitamins and growth factors, and the nutritional value of the fermented feed is remarkably improved. The invention also provides a duckweed fermented product.

The invention belongs to the technical field of gene engineering, and discloses a glomus huanghuai GIP12 protein, a coding gene and application thereof, the amino acid sequence of the glomus huanghuai GIP12 effect protein is shown as SEQ ID NO.2, and the nucleotide sequence of nucleic acid or the gene for coding the effect protein is shown as SEQ ID NO.1. The invention further discloses a preparation method of the glomus huanghuai GIP12 protein. The invention proves that the glomus huanghuai GIP12 protein plays an important role in the process of improving the disease resistance of plants through cloning the glomus huanghuai GIP12 protein gene and transient expression of tobacco.

The invention belongs to the technical field of livestock feed, and discloses a composite boar feed additive and a preparation process thereof. The additive is prepared from tea polyphenol, rosmarinic acid, quercetin, enteric sodium butyrate, yeast cell wall polysaccharide, embedded bacillus subtilis metabolite and a modified starch carrier, the activity of urease and deaminase of posterior intestinal ammonia-producing bacteria is inhibited through multi-component synergism, and nitrogen is regulated and controlled to be converted into mycoprotein. The preparation process comprises the following steps: dissolving and attaching active ingredients, coating enteric-coated pellets, embedding double-layer microcapsules and performing multi-stage mixing. According to the invention, enzyme inhibition functions of tea polyphenol, rosmarinic acid and quercetin are integrated with intestinal barrier strengthening and pH regulation of sodium butyrate, flora regulation of yeast cell wall polysaccharide and targeted bacteriostasis functions of embedded bacillus subtilis metabolites, and accurate release in specific sections of intestinal tracts is realized through enteric coating and microcapsule technologies; on the premise that the protein level of daily ration is not reduced and the reproductive performance is not damaged, the ammonia nitrogen emission is obviously reduced.

The invention relates to the technical field of microorganisms, in particular to a method for preparing microbial protein from soapstock wastewater, and aims to solve the problems of soapstock wastewater pollution and high protein preparation cost. According to the method, soapstock wastewater containing glycerin and sodium acetate is taken as a raw material, and recycling is realized through multi-stage treatment: firstly, toxic impurities are removed through combined detoxification of ferric salt sedimentation filtration, diatomite adsorption and activated carbon adsorption, a nitrogen source, a phosphorus source and growth promoting factors are supplemented after pH is adjusted to construct a balanced nutrition system, and a microbial culture medium is prepared after sterilization; microbial strains are cultured and activated through a liquid seed solution and inoculated into a culture medium for aerated fermentation; and carrying out solid-liquid separation on the fermentation liquor and collecting thalli to obtain microbial mycoprotein, and recycling the separated fermentation waste liquor for nutrition blending. The method realizes unification of efficient purification of the nigre wastewater and preparation of the microbial protein, is environment-friendly in process, low in cost and high in product purity, and has remarkable economic and environmental benefits.

The utility model relates to the technical field of treatment of thallus mash, in particular to a treatment system of thallus protein mash. The treatment system comprises a first vacuum concentration system which comprises a mycoprotein mash buffer tank, a first vacuum concentration device and a concentrated protein mash buffer tank, the mycoprotein mash buffer tank is communicated with the first vacuum concentration device, and the first vacuum concentration device is communicated with the concentrated protein mash buffer tank; the concentrated protein mash buffer tank is communicated with the high-pressure homogenizer; the enzymolysis system comprises a batching tank, an alkali liquor feeding pipe and an enzymolysis tank, the batching tank is communicated with the high-pressure homogenizer, the batching tank is communicated with the enzymolysis tank, and the alkali liquor feeding pipe is communicated with the enzymolysis tank; the purification treatment part is communicated with the enzymolysis tank. According to the treatment system, the first vacuum concentration system 1, the high-pressure homogenizer 2, the enzymolysis system 3 and other operation units are combined, so that the content of peptide substances in the mycoprotein mash can be effectively increased.