230 results about "Protein hydrolysates" patented technology

The present invention relates to a rapeseed protein hydrolysate comprising protein fragments, wherein at least 50% (w / w) of the protein fragments has a molecular weight of less than 3.5 kDa.

The invention belongs to the technical field of deep processing of agricultural products, and provides royal jelly protein zymolyte with melanin inhibition activity and preparation and application of the royal jelly protein zymolyte. Performing enzymolysis on the soluble royal jelly protein solution with pepsin to obtain enzymatic hydrolysate; and carrying out ultrafiltration separation on the enzymatic hydrolysate to obtain a polypeptide solution with the molecular weight of less than 3KDa, namely royal jelly protein hydrolysate. The royal jelly is used as a raw material, the royal jelly protein hydrolysate with low molecular weight is prepared by extracting royal jelly protein and utilizing a protease enzymolysis technology and an ultrafiltration separation technology, and the royal jelly protein hydrolysate has good melanogenesis inhibition activity and can reduce generation of mouse melanoma cells and zebra fish melanin. The inhibitor is derived from natural food royal jelly, is green and safe, is controllable in conditions in the preparation process, is convenient to extract, does not need artificial synthesis, and is expected to be applied to food, medicines or cosmetics with a whitening effect.

The invention discloses a method for preparing, enriching and separating various egg yolk bioactive peptides through enzymolysis of defatted egg yolk powder and application of the egg yolk bioactive peptides, and belongs to the technical field of egg product processing and bioactive peptides. Egg yolk protein hydrolysate is prepared by adopting a three-step enzymolysis process, adsorption and gradient elution are performed on the egg yolk protein hydrolysate by adopting a macroporous resin chromatographic column, four components including water-1, water-2, 20% ethanol and 80% ethanol are obtained, the water-1 component is common egg yolk peptide, the water-1 component is enriched with osteogenic active peptide, the water-2 component is enriched with ethanol, and the 80% ethanol is enriched with ethanol. The 20% ethanol component enriches antioxidant peptide, and the 80% ethanol component enriches ACE inhibitory peptide. The functional activity of each bioactive peptide component is very strong, and the bioactive peptide component can be used as a high-end functional ingredient. According to the method disclosed by the invention, comprehensive utilization of the degreased yolk powder is realized, enrichment and separation of three active peptides are realized by using one raw material through a step-by-step enzymolysis-directional separation technology, the yield of the active peptides is greatly improved, and the economic benefits of enterprises are improved.

The invention discloses a yolk protein source tripeptide with a function of improving osteoporosis and application of the yolk protein source tripeptide, and belongs to the technical field of biological medicines. The tripeptide is a tripeptide APW of which the amino acid sequence is Ala-Pro-Trp. The peptide fragment can be obtained through artificial synthesis or directional enzymolysis of defatted yolk powder. Functional verification shows that the tripeptide APW and the egg yolk protein hydrolysate containing the peptide fragment have the function of improving the bone mineral density, and in a zebra fish osteoporosis model, when the egg yolk protein hydrolysate is 20 mu g / mL, the bone mineral density is improved by 58%; when the tripeptide APW is 0.5 mu g / mL, the bone mineral density is increased by 23.4%; in a rat osteoporosis model, the bone mineral density of thighbone is increased by 3.39% after 10 mg / kg. D of egg yolk protein enzymatic hydrolysate is eaten. Moreover, the food-borne bioactive peptide is high in biological safety, so that the tripeptide APW and a yolk protein enzymolysis product containing the peptide fragment can be applied to preparation of medicines for treating osteoporosis or health-care foods for improving bone mineral density, and the tripeptide APW has a good market prospect and application potential.

The invention discloses an active peptide with effects of resisting oxidation and protecting alcoholic liver injury and application of the active peptide. According to the invention, walnut protein hydrolysate is taken as a raw material, and two novel peptide fragments HGEPGQQQR and VAPFPEVFGK with high antioxidant activity are obtained through screening. The two active peptides show good antioxidant activity on the cellular level, and the EC50 values of the two active peptides are 0.0120 mg / mL and 0.0068 mg / mL respectively, which are both superior to 0.0122 mg / mL of positive control glutathione. In an ethanol injury model, the survival rates of cells subjected to ethanol injury treatment can be respectively increased from 47.35% to 62.69% and 57.05% by the two antioxidant peptides. The results show that the walnut protein source antioxidant peptides HGEPGQQQR and VAPFPEVFGK can be developed and applied as a potential antioxidant and a liver protection active component.

The invention discloses a tortoise shell bioactive peptide and a preparation method and application thereof.The tortoise shell bioactive peptide comprises one or more of EHTVVY, GPKKDQK, SPLSDVRGH, SENADKGALS and DPKMNGLK. The preparation method comprises the steps of preparation of tortoise shell protein hydrolysate, ultrafiltration separation, anion exchange resin separation, gel column separation, de novo sequencing analysis, molecular docking screening and solid-phase synthesis. The tortoise shell bioactive peptide can be applied to the fields of oxidation resistance and immunoregulation. The method disclosed by the invention is a separation, purification and identification technical method of the tortoise shell specific antioxidant polypeptide based on activity guidance, and has the advantages of clear components, clear activity, clear mechanism and the like; the obtained specific polypeptide has important significance for functional product development of polypeptide compounds with clear tortoise shell components.

The invention discloses ACE (Angiotensin Converting Enzyme) inhibitory decapeptide IR10 derived from bovine cheese protein hydrolysate and application of the ACE inhibitory decapeptide IR10, and relates to the technical field of bioactive peptides, and the technical scheme of the ACE inhibitory decapeptide IR10 is characterized in that the amino acid sequence is IQKEDVPSER. The ACE inhibitory decapeptide IR10 derived from the cattle cheese protein hydrolysate is obtained by screening from the cattle cheese protein hydrolysate and is decapeptide IR10 with ACE inhibitory activity, the in-vitro ACE inhibition rate of the ACE inhibitory decapeptide IR10 derived from the cattle cheese protein hydrolysate is tested to be 62.07% under the condition of the concentration of 1mg / ml, and the ACE inhibitory decapeptide IR10 has the effect of being suitable for functional food for assisting blood pressure reduction.

The present invention belongs to the field of cell culture media. Provided are a method for preparing a stem cell culture medium by using a protein hydrolysate as a core raw material and a method of using the stem cell culture medium. The stem cell culture medium contains 20-80% of the protein hydrolysate on the basis of the total weight of the components in the stem cell culture medium. The protein hydrolysate comprises one or more of a high-free amino acid yeast hydrolysate, a high-nucleotide yeast hydrolysate, a high-glutathione yeast hydrolysate, a high-polypeptide yeast hydrolysate, a rice protein hydrolysate, a high-glutamine wheat protein hydrolysate, a pea protein hydrolysate and a soybean protein hydrolysate. The preparation of a natural animal stem cell culture medium by using a whole nutrient protein hydrolysate as a core raw material can meet the nutritional requirements for animal stem cell proliferation under the conditions of no additional addition or addition of a small amount of amino acids, vitamins, growth factors and serum, which effectively reduces the cost of the stem cell culture medium, thereby providing the stem cell culture medium with wide application prospects in the field of foods such as cultured meat.

The invention discloses a method for preparing oil gel by using a protein hydrolysate-cellulose aerogel template, and belongs to the field of biological materials and foods. The method comprises the following steps: (1) preparing cereal protein hydrolysate; (2) preparing a protein hydrolysate-cellulose compound; (3) preparing a protein hydrolysate-cellulose aerogel; (4) carrying out post-treatment on the protein hydrolysate-cellulose aerogel; and (5) preparing the oil gel. According to the method, a protein hydrolysate-cellulose-based composite aerogel is prepared by adopting a dual enhancement strategy of combining electrostatic adsorption and chemical crosslinking, and the oleogel is prepared by taking the composite aerogel as a template through a simple physical oil absorption process. The oleogel is safe, non-toxic and edible, and no toxic reagent is added in the processing process. The oleogel shows a good strain memory effect and remarkable structural elasticity. And excellent oil absorption capacity, oil holding capacity and thermal stability are also shown. The simple method capable of enhancing the mechanical properties of the oleogel is suitable for commercial mass preparation, and the prepared oleogel can be used for replacing fat in artificial meat products.

The present invention discloses a Dendrobium officinale protein hydrolysate composition, a preparation method thereof, and an application thereof in anti-aging cosmetics. The Dendrobium officinale protein hydrolysate composition consists of Dendrobium officinale protein hydrolysates TDM-1 and TDM-5, with a mass ratio of 1:(0.5-2); TDM-1 and TDM-5 are peptide components with a molecular weight ≤10 kDa and a peptide component with a molecular weight of 10 kDa < ≤50 kDa, which are obtained by enzymatic hydrolysis of Dendrobium officinale crude protein using a neutral protease and papain. TDM-1 and TDM-5 components have synergistic effects of antioxidant, anti-inflammatory, and barrier repair by inhibiting the activation of the MAPK / NF-κB signaling pathway and promoting ECM repair mediated by the TGF-β pathway, thereby achieving multi-target anti-aging and having low irritation. They can be used in dosage forms such as essences and creams, providing core raw materials for the development of natural anti-aging cosmetics.

The invention relates to the technical field of biochemistry, in particular to a specific affinity enrichment and preparation method of a torreya grandis protease hydrolysate ACE inhibitory peptide, which comprises the following steps: step 1, preparing and characterizing magnetic aminated Fe3O4 nanoparticles; step 2, preparing and characterizing the immobilized trypsin; 3, preparing and characterizing the immobilized ACE, and optimizing reaction conditions; step 4, determining the activity of the immobilized ACE; step 5, then preparing and specifically enriching the Chinese torreya antihypertensive peptide; step 6, then, carrying out molecular docking through AutoDock Vina; according to the method, genipin is adopted as a cross-linking agent, magnetic Fe3O4 nanoparticles with the ACE inhibitory peptide specific affinity recognition function are constructed, specific enrichment of the ACE inhibitory peptide from the torreya grandis cake protein hydrolysate is achieved, and specific separation is achieved.

The invention provides a dietary fiber composition for reducing weight and regulating intestinal flora in a targeted manner as well as a preparation method and application of the dietary fiber composition. The composition is prepared from the following raw materials in parts by weight: 20 to 30 parts of inulin, 15 to 25 parts of germinated brown rice powder, 10 to 20 parts of pectin, 8 to 15 parts of guar gum, 12 to 20 parts of beta-glucan, 15 to 25 parts of konjac glucomannan, 10 to 20 parts of multi-element dietary fiber powder, 5 to 10 parts of compound probiotic powder, 3 to 8 parts of bitter gourd polypeptide, 3 to 8 parts of folium mori extract and 2 to 5 parts of protein hydrolysate. Multi-element dietary fiber powder, prebiotics and the like are combined, endogenous GLP-1 is stimulated, the characteristics that the protein nutrient is not easily absorbed by people, but can be utilized by intestinal flora to generate SCFA and stimulate GLP-1 secreted by L cells at the far end of the intestinal tract are fully exerted, the requirements of most people for weight losing and weight control can be met, the protein nutrient can serve as a source of DDP-IV inhibitory peptide, decomposition of the endogenous GLP-1 is slowed down, and the weight losing and weight control effects are achieved. A more lasting effect of endogenous GLP-1 is ensured; a hot extrusion embedding technology and an airflow cutting technology are adopted, so that more nutrient substances can be effectively reserved in intestinal tracts at the same time, and nutrients are provided for flora.

The invention relates to methods for the preparation of protein hydrolysates having low concentrations of undesired nitrosamines as well as the thus obtained protein hydrolysates, compositions containing them and uses thereof. The methods comprise the steps of (a) contacting at least one protein with at least one oxidant, preferably a peroxide, at a concentration of 10 to <500 ppm relative to the weight of the at least one protein, at a pH in the range of 2.0 to <7.0; and (b) subjecting the at least one protein of step (a) to a hydrolysis reaction to obtain a protein hydrolysate.

The invention provides an antioxidant peptide as well as a preparation method and application thereof, and belongs to the technical field of biology. The invention provides an antioxidant peptide with an amino acid sequence as shown in SEQ ID NO. 2 and / or an antioxidant peptide with an amino acid sequence as shown in SEQ ID NO. 4. Two antioxidative peptides are identified from the gastrodia elata protein hydrolysate through enzymolysis, ultrafiltration, gel chromatography purification and mass spectrometry technologies in combination with ABTS and DPPH free radical scavenging activity, the molecular weights of the two antioxidative peptides are 937.08 Da and 1153.50 Da respectively, and the two antioxidative peptides have high antioxidative activity, heat stability and in-vitro safety and can be used for preparing antioxidative peptides for the gastrodia elata protein hydrolysate. The cell viability decline and ROS level rise caused by oxidative stress injury of cells can be effectively relieved, and the neuroprotective effect can be achieved by activating an Nrf2 pathway. The antioxidant peptide is suitable for daily chemical products, skin care and beauty cosmetics, health foods and medicines with antioxidant efficacy requirements.

The invention relates to the technical field of small molecule peptide royal jelly production control, in particular to a small molecule peptide royal jelly production control method and system. Comprising the following steps: applying preset tiny pH disturbance to an enzymolysis reaction solution to obtain a pH change curve, and extracting transient response performance data; acquiring at least one auxiliary response data, and performing cross validation on the transient response performance data and the auxiliary response data to obtain a corrected pH value; monitoring the generation rate of the protein hydrolysate, and obtaining a reversely deduced pH value according to a pre-established enzyme pH-activity relation model; calculating a pH measurement reliability index based on the transient response performance data, the corrected pH value and the inversely deduced pH value; and self-adaptive adjustment is carried out according to the pH measurement reliability index. The problems of measurement deviation caused by aging of the pH probe in the production of the small molecule peptide royal jelly, reduction of the enzymolysis efficiency, damage to the product quality, increase of the production cost and the like caused by the measurement deviation are solved.

To provide a beverage or food in a sealed container which comprises protein hydrolysate, caffeine, and polyphenols and exhibit high long-term preservation stability, and a method for producing the beverage or food in a sealed container.SOLUTION: A beverage or food in a sealed container comprising: a sealed container; and a beverage or food housed in the sealed container, the beverage or food containing caffeine and polyphenols, wherein the beverage or food comprises a protein hydrolysate, and wherein the dissolved oxygen content of the beverage or food at 10°C is 4.0 mg / L or less. The volume of the beverage or food exceeds 99.9 vol.% relative to the volume of the sealed container.SELECTED DRAWING: Figure 1

The application discloses a composition, which comprises hydrolyzed protein; the hydrolyzed protein comprises hydrolyzed whey protein and hydrolyzed casein protein, the weight ratio of the hydrolyzed whey protein and the hydrolyzed casein protein is (70-95):(5-30), the amino acid nitrogen content in the hydrolyzed protein is (1.0-3.0) g / 100 g, the proportion of protein hydrolysate with a relative molecular mass distribution <1000 in the hydrolyzed protein is 34-70%, the peptide content (calculated on a dry basis) in the hydrolyzed protein is not less than 33 g / 100 g, and the total free amino acid content in the hydrolyzed protein is not less than 1 g / 100 g. The composition can effectively treat diarrhea and is suitable for people with gastrointestinal dysfunction and severe patients with diarrhea symptoms.

The present invention relates to a protein hydrolysate comprising L-cysteine-containing peptides, methods for obtaining it and uses of the hydrolysate.

Belongs to the field of cell culture media. The stem cell culture medium contains 20-80% of protein hydrolysate on the basis of the weight of the total components in the stem cell culture medium. The protein hydrolysate comprises one or more of a high free amino acid yeast hydrolysate, a high nucleotide yeast hydrolysate, a high glutathione yeast hydrolysate, a high polypeptide type yeast hydrolysate, a rice protein hydrolysate, a high glutamine wheat protein hydrolysate, a pea protein hydrolysate and a soybean protein hydrolysate. The animal stem cell natural culture medium is prepared by taking the full-nutrition protein hydrolysate as a core raw material, so that the nutritional requirements of animal stem cell proliferation can be met under the condition of not additionally adding or adding a small amount of amino acids, vitamins, growth factors and serum, and the cost of the stem cell culture medium is effectively reduced; therefore, the method has a wide application prospect in the field of food such as cell culture meat.

The invention discloses a hydrolysate deacidification-dehydration coupling process and a byproduct recovery method, and belongs to the technical field of hydrolysate treatment. The method comprises the following steps: pretreating a hydrolysate raw material through two-stage filtration; adding the pretreated hydrolysate, a deacidification agent and a dehydration auxiliary agent into a coupling reaction device, and synchronously carrying out deacidification-dehydration reaction under the conditions of 60-120 DEG C and 0.1-0.5 MPa; after the reaction, separating to obtain a deacidification and dehydration product and a mixed solid phase; and dissolving and crystallizing the mixed solid phase to recover high-purity byproducts. The deacidification and dehydration synergistic interaction is achieved, the acidity reduction rate is larger than or equal to 85%, the water content is smaller than or equal to 5%, and the process time consumption is shortened by 28.6%; the byproduct purity is not less than 90%, and the recovery rate is not less than 80 A tail gas treatment system is additionally arranged to ensure that environmental protection reaches the standard. The process is suitable for biomass, polysaccharide and protein hydrolysate treatment, has the advantages of high efficiency, good resource utilization rate and the like, and provides an efficient solution for hydrolysate industrial treatment.

A method of preserving the pollen viability of maize, especially under heat stress, comprising treating the maize plants with a protein hydrolysate.

A wet pet food palatability enhancer includes: a palatability agent component selected from a group consisting of a protein hydrolysate and a yeast extract; a free glycine; and a reducing sugar. A phosphorus content of the wet pet food palatability enhancer is 1% or less in terms of dry matter. The wet pet food palatability enhancer does not include a Maillard reaction product.

Protein hydrolysate compositions and methods of making the same are disclosed. The protein hydrolysate composition has a protein-rich organic content. The protein hydrolysate composition may be substantially free of exogenous chelating agents, chaotropic agents and surfactants. The protein hydrolysate composition may be low in ash content. The protein hydrolysate composition is produced by processing a biomass, e.g., a microbial biomass, through a combination of physical, chemical and / or enzymatic treatments. The protein hydrolysate may be sourced via microbial biomass from CCk as a carbon source. Also disclosed are methods of using the protein hydrolysate compositions, e.g., as a biostimulant.

[Problem] To provide a matsutake primordium having a high methyl cinnamate content and a method capable of obtaining such a matsutake primordium having a high methyl cinnamate content at an artificially high yield. [Solution] Cells having the ability to form a primordium that have been isolated from matsutake fruiting bodies are cultured in a medium containing a protein degradation product and having a specific phenylalanine concentration.

The invention discloses a composition containing pig cerebroprotein hydrolysate, and a preparation method and application thereof. The composition comprises pig brain protein hydrolysate, gastrodia elata, American ginseng, dogwood, ginkgo, rhizoma polygonati, radix puerariae, folium mori, radix asparagi, vitamin C, vitamin B1, vitamin B3, vitamin B5, vitamin B6 and vitamin B12. The composition can improve memory, further repair damaged nerve cells, improve brain blood circulation, reduce damage caused by oxygen free radicals, accelerate removal of free radicals in the body, eliminate inflammation and maintain nervous system health, so that the composition has the effects of improving memory and preventing Alzheimer's disease.

Problem: To provide a cell culture substrate, a cell culture vessel, and a cell detachment method for adherent cells, which enable easier detachment of adherent cells compared to a case where trypsin is used. Solution: A cell culture substrate for coating a cell culture vessel, the substrate containing, as an active ingredient, one or more proteins of a silk protein, a silk protein hydrolysate, fibroin, and a fibroin hydrolysate.

The invention discloses selenium-containing chickpea peptide, a preparation method thereof and application of the selenium-containing chickpea peptide in preparation of antihypertensive drugs, and belongs to the technical field of bioactive peptides. According to the method, selenium-rich bacillus natto is used for fermenting chickpeas to obtain selenium-containing chickpea protein hydrolysate, and then the selenium-containing chickpea peptides PQM and PQML are obtained through fermentation condition optimization and a quantitative peptidomics technology. Experiments prove that the selenium-containing chickpea peptide has good in-vivo stability, bioavailability and antihypertensive activity, has the effect of inhibiting the activity of angiotensin converting enzyme (ACE), and has the potential of preventing or treating hypertension.

The application discloses a controllable preparation method of high-activity earthworm protein peptide and belongs to the technical field of earthworm processing. The preparation method comprises the following steps: (1) adding amylase and cutinase to earthworm paste for hydrolysis; (2) filtering by using a filter; (3) hydrolyzing the filtrate by using a composite enzyme; (4) adding alkaline protease to the filter residue for hydrolysis, and then adding high-temperature protease for hydrolysis; (5) filtering the hydrolyzed paste, and then filtering and treating the paste by using a ceramic microfilter to obtain low-molecular-weight earthworm protein peptide; and (6) mixing the obtained earthworm water-soluble protein hydrolysate and the obtained earthworm insoluble protein polypeptide, and then concentrating and drying to obtain high-activity earthworm protein peptide. According to the application, the water-soluble protein and the water-insoluble protein in the earthworm are step-by-step differentially hydrolyzed, the protein resources in the earthworm are maximally utilized, the polypeptide / protein yield can be more than 85%, waste is avoided, and the activity of the obtained protein peptide is ensured.

The present disclosure relates to an integrated process for obtaining ethanol and proteins from cereal grains (broken rice), the process comprising milling the grains, obtaining a slurry of the milled grains, partially hydrolysing the slurry, saccharifying the partially hydrolysed slurry to obtain saccharified slurry, adding a pre-fermented slurry to the saccharified slurry to obtain ethanol and distilling out the ethanol and separating the remaining as a whole stillage; separating the whole stillage into thin slop and DWG, treating a slurry of the DWG with cellulase enzymes to provide treated DWG slurry, separating the treated DWG slurry into wet cake and filtrate, washing and separating the said wet cake into protein concentrate and a liquid and optionally treating the obtained protein concentrate with alkali or hydrolysing enzymes to obtain purified protein hydrolysate or isolate.

The invention discloses a frozen dough modifier, which is prepared by the following steps: by taking sea buckthorn pomace as a raw material, firstly performing phytase treatment and alkali-solution and acid-precipitation to obtain sea buckthorn pomace protein, and then performing enzymolysis treatment by alkaline protease to obtain sea buckthorn pomace protein hydrolysate as a freezing modifier. The frozen dough improver contains 64% of hydrophilic amino acid, can provide a large number of hydrogen bonds for dough, enhances the acting force between the dough and water molecules, and further effectively inhibits growth of ice crystals and recrystallization, thereby preventing physical damage of large ice crystals to the dough structure. The improver has the advantages of remarkably enhancing the water binding capacity, the fermentation capacity, the resistance and the ductility of the dough, and meanwhile bread made of the frozen dough containing the improver is large in specific volume, soft in tissue and better in quality.