146 results about "Protein hydrolysates" patented technology

The invention discloses a method for preparing, enriching and separating various egg yolk bioactive peptides through enzymolysis of defatted egg yolk powder and application of the egg yolk bioactive peptides, and belongs to the technical field of egg product processing and bioactive peptides. Egg yolk protein hydrolysate is prepared by adopting a three-step enzymolysis process, adsorption and gradient elution are performed on the egg yolk protein hydrolysate by adopting a macroporous resin chromatographic column, four components including water-1, water-2, 20% ethanol and 80% ethanol are obtained, the water-1 component is common egg yolk peptide, the water-1 component is enriched with osteogenic active peptide, the water-2 component is enriched with ethanol, and the 80% ethanol is enriched with ethanol. The 20% ethanol component enriches antioxidant peptide, and the 80% ethanol component enriches ACE inhibitory peptide. The functional activity of each bioactive peptide component is very strong, and the bioactive peptide component can be used as a high-end functional ingredient. According to the method disclosed by the invention, comprehensive utilization of the degreased yolk powder is realized, enrichment and separation of three active peptides are realized by using one raw material through a step-by-step enzymolysis-directional separation technology, the yield of the active peptides is greatly improved, and the economic benefits of enterprises are improved.

The invention discloses a yolk protein source tripeptide with a function of improving osteoporosis and application of the yolk protein source tripeptide, and belongs to the technical field of biological medicines. The tripeptide is a tripeptide APW of which the amino acid sequence is Ala-Pro-Trp. The peptide fragment can be obtained through artificial synthesis or directional enzymolysis of defatted yolk powder. Functional verification shows that the tripeptide APW and the egg yolk protein hydrolysate containing the peptide fragment have the function of improving the bone mineral density, and in a zebra fish osteoporosis model, when the egg yolk protein hydrolysate is 20 mu g / mL, the bone mineral density is improved by 58%; when the tripeptide APW is 0.5 mu g / mL, the bone mineral density is increased by 23.4%; in a rat osteoporosis model, the bone mineral density of thighbone is increased by 3.39% after 10 mg / kg. D of egg yolk protein enzymatic hydrolysate is eaten. Moreover, the food-borne bioactive peptide is high in biological safety, so that the tripeptide APW and a yolk protein enzymolysis product containing the peptide fragment can be applied to preparation of medicines for treating osteoporosis or health-care foods for improving bone mineral density, and the tripeptide APW has a good market prospect and application potential.

The present invention discloses a Dendrobium officinale protein hydrolysate composition, a preparation method thereof, and an application thereof in anti-aging cosmetics. The Dendrobium officinale protein hydrolysate composition consists of Dendrobium officinale protein hydrolysates TDM-1 and TDM-5, with a mass ratio of 1:(0.5-2); TDM-1 and TDM-5 are peptide components with a molecular weight ≤10 kDa and a peptide component with a molecular weight of 10 kDa < ≤50 kDa, which are obtained by enzymatic hydrolysis of Dendrobium officinale crude protein using a neutral protease and papain. TDM-1 and TDM-5 components have synergistic effects of antioxidant, anti-inflammatory, and barrier repair by inhibiting the activation of the MAPK / NF-κB signaling pathway and promoting ECM repair mediated by the TGF-β pathway, thereby achieving multi-target anti-aging and having low irritation. They can be used in dosage forms such as essences and creams, providing core raw materials for the development of natural anti-aging cosmetics.

The invention relates to the technical field of small molecule peptide royal jelly production control, in particular to a small molecule peptide royal jelly production control method and system. Comprising the following steps: applying preset tiny pH disturbance to an enzymolysis reaction solution to obtain a pH change curve, and extracting transient response performance data; acquiring at least one auxiliary response data, and performing cross validation on the transient response performance data and the auxiliary response data to obtain a corrected pH value; monitoring the generation rate of the protein hydrolysate, and obtaining a reversely deduced pH value according to a pre-established enzyme pH-activity relation model; calculating a pH measurement reliability index based on the transient response performance data, the corrected pH value and the inversely deduced pH value; and self-adaptive adjustment is carried out according to the pH measurement reliability index. The problems of measurement deviation caused by aging of the pH probe in the production of the small molecule peptide royal jelly, reduction of the enzymolysis efficiency, damage to the product quality, increase of the production cost and the like caused by the measurement deviation are solved.

To provide a beverage or food in a sealed container which comprises protein hydrolysate, caffeine, and polyphenols and exhibit high long-term preservation stability, and a method for producing the beverage or food in a sealed container.SOLUTION: A beverage or food in a sealed container comprising: a sealed container; and a beverage or food housed in the sealed container, the beverage or food containing caffeine and polyphenols, wherein the beverage or food comprises a protein hydrolysate, and wherein the dissolved oxygen content of the beverage or food at 10°C is 4.0 mg / L or less. The volume of the beverage or food exceeds 99.9 vol.% relative to the volume of the sealed container.SELECTED DRAWING: Figure 1

The application discloses a composition, which comprises hydrolyzed protein; the hydrolyzed protein comprises hydrolyzed whey protein and hydrolyzed casein protein, the weight ratio of the hydrolyzed whey protein and the hydrolyzed casein protein is (70-95):(5-30), the amino acid nitrogen content in the hydrolyzed protein is (1.0-3.0) g / 100 g, the proportion of protein hydrolysate with a relative molecular mass distribution <1000 in the hydrolyzed protein is 34-70%, the peptide content (calculated on a dry basis) in the hydrolyzed protein is not less than 33 g / 100 g, and the total free amino acid content in the hydrolyzed protein is not less than 1 g / 100 g. The composition can effectively treat diarrhea and is suitable for people with gastrointestinal dysfunction and severe patients with diarrhea symptoms.

Belongs to the field of cell culture media. The stem cell culture medium contains 20-80% of protein hydrolysate on the basis of the weight of the total components in the stem cell culture medium. The protein hydrolysate comprises one or more of a high free amino acid yeast hydrolysate, a high nucleotide yeast hydrolysate, a high glutathione yeast hydrolysate, a high polypeptide type yeast hydrolysate, a rice protein hydrolysate, a high glutamine wheat protein hydrolysate, a pea protein hydrolysate and a soybean protein hydrolysate. The animal stem cell natural culture medium is prepared by taking the full-nutrition protein hydrolysate as a core raw material, so that the nutritional requirements of animal stem cell proliferation can be met under the condition of not additionally adding or adding a small amount of amino acids, vitamins, growth factors and serum, and the cost of the stem cell culture medium is effectively reduced; therefore, the method has a wide application prospect in the field of food such as cell culture meat.

The invention discloses a hydrolysate deacidification-dehydration coupling process and a byproduct recovery method, and belongs to the technical field of hydrolysate treatment. The method comprises the following steps: pretreating a hydrolysate raw material through two-stage filtration; adding the pretreated hydrolysate, a deacidification agent and a dehydration auxiliary agent into a coupling reaction device, and synchronously carrying out deacidification-dehydration reaction under the conditions of 60-120 DEG C and 0.1-0.5 MPa; after the reaction, separating to obtain a deacidification and dehydration product and a mixed solid phase; and dissolving and crystallizing the mixed solid phase to recover high-purity byproducts. The deacidification and dehydration synergistic interaction is achieved, the acidity reduction rate is larger than or equal to 85%, the water content is smaller than or equal to 5%, and the process time consumption is shortened by 28.6%; the byproduct purity is not less than 90%, and the recovery rate is not less than 80 A tail gas treatment system is additionally arranged to ensure that environmental protection reaches the standard. The process is suitable for biomass, polysaccharide and protein hydrolysate treatment, has the advantages of high efficiency, good resource utilization rate and the like, and provides an efficient solution for hydrolysate industrial treatment.

Protein hydrolysate compositions and methods of making the same are disclosed. The protein hydrolysate composition has a protein-rich organic content. The protein hydrolysate composition may be substantially free of exogenous chelating agents, chaotropic agents and surfactants. The protein hydrolysate composition may be low in ash content. The protein hydrolysate composition is produced by processing a biomass, e.g., a microbial biomass, through a combination of physical, chemical and / or enzymatic treatments. The protein hydrolysate may be sourced via microbial biomass from CCk as a carbon source. Also disclosed are methods of using the protein hydrolysate compositions, e.g., as a biostimulant.

[Problem] To provide a matsutake primordium having a high methyl cinnamate content and a method capable of obtaining such a matsutake primordium having a high methyl cinnamate content at an artificially high yield. [Solution] Cells having the ability to form a primordium that have been isolated from matsutake fruiting bodies are cultured in a medium containing a protein degradation product and having a specific phenylalanine concentration.

The invention discloses selenium-containing chickpea peptide, a preparation method thereof and application of the selenium-containing chickpea peptide in preparation of antihypertensive drugs, and belongs to the technical field of bioactive peptides. According to the method, selenium-rich bacillus natto is used for fermenting chickpeas to obtain selenium-containing chickpea protein hydrolysate, and then the selenium-containing chickpea peptides PQM and PQML are obtained through fermentation condition optimization and a quantitative peptidomics technology. Experiments prove that the selenium-containing chickpea peptide has good in-vivo stability, bioavailability and antihypertensive activity, has the effect of inhibiting the activity of angiotensin converting enzyme (ACE), and has the potential of preventing or treating hypertension.

The application discloses a controllable preparation method of high-activity earthworm protein peptide and belongs to the technical field of earthworm processing. The preparation method comprises the following steps: (1) adding amylase and cutinase to earthworm paste for hydrolysis; (2) filtering by using a filter; (3) hydrolyzing the filtrate by using a composite enzyme; (4) adding alkaline protease to the filter residue for hydrolysis, and then adding high-temperature protease for hydrolysis; (5) filtering the hydrolyzed paste, and then filtering and treating the paste by using a ceramic microfilter to obtain low-molecular-weight earthworm protein peptide; and (6) mixing the obtained earthworm water-soluble protein hydrolysate and the obtained earthworm insoluble protein polypeptide, and then concentrating and drying to obtain high-activity earthworm protein peptide. According to the application, the water-soluble protein and the water-insoluble protein in the earthworm are step-by-step differentially hydrolyzed, the protein resources in the earthworm are maximally utilized, the polypeptide / protein yield can be more than 85%, waste is avoided, and the activity of the obtained protein peptide is ensured.

The invention discloses a molecular docking screening method and application of hermetia illucens protein antioxidant peptides, and relates to the technical field of biologication.The method comprises the following steps that S1, hermetia illucens protein is prepared through enzymolysis, wherein hermetia illucens defatted protein powder serves as a raw material, enzymolysis is conducted through pepsin under the enzymolysis conditions that the pH is 2.0, the temperature is 40 DEG C, the enzyme adding amount is 3% of the protein mass, the enzymolysis time is 3 h, and enzymolysis liquid is obtained; after the reaction is finished, inactivating, centrifuging, collecting supernatant, and freeze-drying to obtain hermetia illucens protein hydrolysate; according to the molecular docking screening method and application of the hermetia illucens protein antioxidant peptide, the molecular docking technology is specifically applied to efficient screening of the hermetia illucens-sourced antioxidant peptide, KEAP1 is taken as a key target, high-activity candidate peptide is rapidly and accurately positioned from massive enzymolysis peptide fragments, and the screening efficiency of the hermetia illucens-sourced antioxidant peptide is improved. The defects that a traditional blind screening method is large in workload, long in period and high in cost are overcome, and the screening efficiency and the success rate are remarkably improved.

The invention relates to the technical field of food processing, in particular to an alkali-induced glycosylated tilapia protein hydrolysate loaded with fat-soluble polyphenol as well as a preparation method and application of the alkali-induced glycosylated tilapia protein hydrolysate. The glycosylated tilapia mossambica protein hydrolysate is prepared by adopting flavourzyme with mild action conditions for moderate hydrolysis and combining with a green, efficient and reaction degree-adjustable alkali-induced propylene glycol alginate (PGA) glycosylation strategy, so that the water solubility and emulsibility of tilapia mossambica protein are effectively improved, and the tilapia mossambica protein hydrolysate can be used for preparing the tilapia mossambica protein hydrolysate. The glycosylated protein hydrolysate (modified tilapia protein) is used as a carrier to develop an oral delivery system of fat-soluble polyphenols such as quercetin and the like, so that the bioaccessibility of the fat-soluble polyphenols is improved, and the fat-soluble polyphenols can be developed into functional food ingredients to improve the health condition of special groups; the high-value utilization of the tilapia protein in the protein-based polyphenol functional food ingredients is promoted.

The application discloses selenium-containing chickpea peptides, a preparation method thereof and application of the selenium-containing chickpea peptides in preparation of blood pressure lowering drugs, and belongs to the technical field of bioactive peptides. Selenium-enriched Bacillus natto is used to ferment chickpeas to obtain selenium-containing chickpea protein hydrolysate, and then fermentation conditions are optimized and quantitative peptidomics technology is used to obtain selenium-containing chickpea peptides PQM and PQML. Experiments prove that the selenium-containing chickpea peptides have good in-vivo stability, bioavailability and blood pressure lowering activity, have an effect of inhibiting angiotensin converting enzyme (ACE) activity, and have potential for preventing or treating hypertension.

FISH PROTEIN HYDROLYSATE AND APPLICATIONS IN FARM ANIMALS The present invention relates to a protein hydrolysate originating from fish tissues or cartilages rich in protein, in particular collagen, and its applications in the feeding and welfare of farm animals, in particular pigs and poultry. In particular, the invention relates to a hydrolysate obtained by enzymatic digestion using an endopeptidase enzyme, said hydrolysate being characterized in that it has a protein fraction having a molecular profile with the following distribution: between 15 and 60% of peptides with a molecular weight of between 1000 and 5000 Da, between 20 and 40% of peptides with a molecular weight of between 500 and 1000 Da, between 10 and 40% of peptides with a molecular weight of between 150 and 500 Da, between 1 and 10% of peptides with a molecular weight of between 1000 and 50 ... molecular weight less than 150 Da. Fig. 2

Structured food compositions, such as meat analogue compositions, which include protein products from microorganisms, such as microorganism-derived protein hydrolysates, are described. Methods of making such products are also described.

The invention discloses a walnut source DPP-IV inhibitory peptide as well as a preparation and screening method and application thereof. The walnut source DPP-IV inhibitory peptide comprises FPAG and / or LPSYQPTP, the preparation and screening method comprises the following steps: (1) adding walnut protein powder into deionized water, and fully dissolving to obtain a walnut protein solution; (2) adding hydrolyzed compound protease into the walnut protein solution for enzymolysis to obtain walnut protein hydrolysate freeze-dried powder; (3) re-suspending the walnut protein hydrolysate freeze-dried powder in deionized water, and performing ultrafiltration separation by using an ultrafiltration membrane with the molecular weight cutoff of 1kDa to obtain a component F1; step (4), performing peptide fragment sequence identification on the obtained F1 component through peptidomics; and step (5), screening from peptide fragment sequences obtained by identification through a bioinformatics technology. The walnut source DPP-IV inhibitory peptide screened by the invention can be used for preparing a medicine or food with a DPP-IV inhibition function, so that the problems that the existing walnut source DPP-IV inhibitor has many side effects and the like are solved.

The present invention relates to a protein hydrolysate, wherein at least 90 wt.% of the peptides in the protein hydrolysate have a molecular mass of at most 500 Dalton, and wherein between 35 and 55 wt.% of the peptides have a molecular mass between 204 and 300 Dalton.

The invention belongs to the technical field of corn protein processing, and particularly relates to corn enzymolysis protein with high protein content and high micromolecule active substances and a preparation method. The method comprises the following steps: S1, weighing corn protein powder, adding water into the corn protein powder, stirring, adding alkali to adjust the pH value, and then adding medium-temperature amylase for enzymolysis; s2, separating the materials to obtain filter residues; s3, adding water into the materials, stirring, and adding acidic cellulase for enzymolysis; and S4, raising the temperature of the material, adjusting the pH value with alkali, and adding alkaline protease for enzymolysis. The corn enzymolysis protein prepared by the method can be used as a high-quality functional protein to be applied to aquatic feed, so that the intestinal and liver health of aquatic animals is remarkably improved, the total dosage of protein raw materials in the aquatic feed is reduced, and the dosage of imported protein resources (such as fish meal) is reduced; pigmentation can be promoted, and the commodity performance of aquatic animals can be improved; therefore, the economic benefits of aquatic feed enterprises and farmers are improved.

The present invention relates to a method for obtaining a specific protein hydrolysate from Moringa oleifera seed cake. The present invention also relates to a protein hydrolysate from Moringa oleifera seed cake and its use as a medicament. Finally, the present invention relates to a pharmaceutical or dermatological composition comprising an effective amount of a protein hydrolysate from Moringa oleifera seed cake as an active agent, for its use as a medicament for treating fibrotic diseases and inflammatory conditions, treating cancer, treating bacterial or viral infectious diseases, and treating genetic drift and pathologies associated with skin pigmentation.

The invention discloses a method for inducing aroma enhancement of egg yolk hydrolysate by utilizing Maillard reaction, and belongs to the technical field of food processing. The method disclosed by the invention comprises the following steps: taking fresh egg yolk, homogenizing, adding water, adjusting the pH value to 7-8, respectively adding protease and cysteine endonuclease for reaction, and taking supernatant after the reaction, thereby obtaining the aroma-enhanced egg yolk protein hydrolysate. Then xylose is added, the initial pH is adjusted to 7.0-7.5, and homogenization treatment is performed; and carrying out spray drying on the homogenized system to obtain powder, placing the powder in a sealed container, and carrying out a dry heat reaction to obtain the aroma-enhanced egg yolk hydrolysate. The egg yolk hydrolysate is rich in characteristic volatile compounds such as furans, pyrazines, aldehydes and the like, presents rich toast bread fragrance, nut fragrance, soft meat fragrance and scorch fragrance, has excellent oxidation resistance, and improves flavor and optimizes taste experience through synergism of amino acid and nucleotide; the product is used as a natural flavor base material for food processing of seasonings, meat products, egg products and the like, the process is simple and controllable, and the method is suitable for industrial production.

The present invention relates to a method for preparing an eco-friendly fertilizer by using a by-product produced during starfish extraction, and an eco-friendly fertilizer prepared thereby. According to the present invention, the eco-friendly liquid fertilizer can be prepared through a simple process by using waste liquid remaining after solids are collected from starfish protein hydrolysates. The fertilizer according to the present invention has an excellent composition of organic matter and nitrogen-phosphate-potassium (NPK) and exhibits growth-promoting activity due to starfish-derived organic matter, and thus, if the fertilizer of the present invention is used during crop cultivation, the quality and yield of crops can be effectively improved.

The invention relates to an isolated peptide having an amino acid sequence selected from the group consisting of: Pro-Val-Leu-Lys (SEQ ID NO: 1); Pro-Leu-Pro-Arg (SEQ ID NO: 2); Glu-Ala-Glu-Phe-Asp (SEQ ID NO: 3); Pro-Glu-Arg-Asn-Glu (SEQ ID NO: 4); Pro-Gln-Pro-Glu-Arg (SEQ ID NO: 5); Glu-Phe-Asp-Glu-Lys-Pro-Ala-Asp (SEQ ID NO: 6); Glu-Asp-Ala-Pro-Val-Leu-His (SEQ ID NO: 7); Glu-Phe-Asp-Ala-Arg-Pro-Thr (SEQ ID NO: 8); Glu-Glu-Thr-Pro-Ser-His-Asp (SEQ ID NO: 9); Glu-Glu-Val-Glu-Glu-Glu-Glu-Val-Glu (SEQ ID NO: 10); Glu-Phe-Asp-Glu-Lys-Ala-Pro-Asp (SEQ ID NO: 11); Glu-Asn-Ala-Pro-Asp-Gln-Lys (SEQ ID NO: 12); Glu-Asp-Met-Ala-Pro (SEQ ID NO: 13); Pro-Ile-Asn-Asp-Asn (SEQ ID NO: 14); Pro-Thr-Asp-Leu (SEQ ID NO: 15); Pro-Val-Ala-Glu (SEQ ID NO: 16); Arg-Asn-Gly-Pro-Arg (SEQ ID NO: 17); Arg-Ala-Glu-Asp-Thr-Ala-Thr-Tyr-Tyr (SEQ ID NO: 18); Glu-Gly-Asp-Ser-Ala-Ala-Ile (SEQ ID NO: 19); Glu-Pro-Glu-Gly-Asp-Val-His-Gln (SEQ ID NO: 20); and Glu-Asp-Glu-Val-Leu-Ala-Thr-Pro (SEQ ID NO: 21), a protein hydrolysate comprising the isolated peptides, and use thereof as a taste-modulating agent in food and pharmaceutical preparations.

The invention relates to a proteolysis product with a gastric mucosa protection effect, a micromolecular proteolysis product and application. The present invention relates to a proteolysis product having a gastric mucosa protective effect, which is obtained by performing enzymolysis on a mycoprotein dispersion liquid using papain to obtain a solution in which the proteolysis product having the gastric mucosa protective effect is dispersed. The proteolysis product provided by the invention has a gastric mucosa protection effect, and especially has obvious protection and repair effects on ethanol-induced gastric mucosa injury; in addition, the raw materials are easy to obtain, safe and reliable, the preparation method is simple, no harmful substance is introduced, and batch production is easy. The proteolysis product provided by the invention has homology of medicine and food, also has a gastric mucosa protection effect, and can be used as a health-care product with an auxiliary protection function on gastric mucosa.

The invention belongs to the field of microorganisms and food processing, and particularly discloses a fungal protein hydrolysate, a preparation method thereof and application of the fungal protein hydrolysate in meat flavor. According to the method disclosed by the invention, firstly, fungi are treated by a combined means of alkali liquor suspension, high-pressure homogenization and heating, so that the extractability of fungal protein is improved; then, protease is adopted for carrying out directional cutting on hydrophobic amino acid residue sites, and the protein hydrolysate rich in branched chain amino acid is obtained. The hydrolysate has the following advantages: (1) the content of branched chain amino acids (leucine, isoleucine and valine) is high; the total content of branched chain amino acids accounts for more than 20% of the total content of free amino acids; (2) the proportion of low-molecular-weight peptide is high; the mass percentage content of the protein peptide fragment with the molecular weight of less than 3 kD is not less than 80%; and (3) good Maillard reaction activity is achieved, and rich meat flavor substances can be generated under the heating condition.

The application provides a lacto-casein hydrolysate with anti-inflammatory effect and a preparation method and application thereof.The lacto-casein hydrolysate of the application comprises a peptide segment FAWPQYLK, a peptide segment LPW, a peptide segment MPLW and a peptide segment PLW, can reduce the release of pro-inflammatory cytokines, such as IL-1beta, IL-2, IL-6 and TNF-alpha, and improve the release of anti-inflammatory cytokines, such as IL-10, so as to reduce the cell inflammatory response, and further achieve the anti-inflammatory effect.

The invention relates to the field of traditional Chinese medicine hair care, and discloses a multi-protein-containing repairing hair mask and a preparation method thereof. The hair mask is prepared from silk protein hydrolysate, wheat protein peptide, collagen tripeptide and polygonum multiflorum / cacumen biotae / ginseng traditional Chinese medicine extract according to a specific proportion through a step-by-step enzymolysis and low-temperature emulsification process. The multi-protein system can permeate into the deep layer of hair epidermis to repair damaged hair cuticles under the synergism of the active ingredients of the traditional Chinese medicines. Compared with a traditional single-protein hair mask, the product has the advantages of remarkable repair depth and lasting effect, can effectively improve the strength and glossiness of hair, and is suitable for caring hair damaged by ironing and dyeing.

The present invention relates to a process that, by using as raw materials by-products from the agribusiness industry, such as waste biomass from the processing of food flours, enables chemical intermediates and protein hydrolysates with biostimulant action for plants to be produced from the polysaccharides and proteins present in such biomass.