93 results about "Protein hydrolysates" patented technology

The invention relates to the technical field of small molecule peptide royal jelly production control, in particular to a small molecule peptide royal jelly production control method and system. Comprising the following steps: applying preset tiny pH disturbance to an enzymolysis reaction solution to obtain a pH change curve, and extracting transient response performance data; acquiring at least one auxiliary response data, and performing cross validation on the transient response performance data and the auxiliary response data to obtain a corrected pH value; monitoring the generation rate of the protein hydrolysate, and obtaining a reversely deduced pH value according to a pre-established enzyme pH-activity relation model; calculating a pH measurement reliability index based on the transient response performance data, the corrected pH value and the inversely deduced pH value; and self-adaptive adjustment is carried out according to the pH measurement reliability index. The problems of measurement deviation caused by aging of the pH probe in the production of the small molecule peptide royal jelly, reduction of the enzymolysis efficiency, damage to the product quality, increase of the production cost and the like caused by the measurement deviation are solved.

The application discloses a composition, which comprises hydrolyzed protein; the hydrolyzed protein comprises hydrolyzed whey protein and hydrolyzed casein protein, the weight ratio of the hydrolyzed whey protein and the hydrolyzed casein protein is (70-95):(5-30), the amino acid nitrogen content in the hydrolyzed protein is (1.0-3.0) g / 100 g, the proportion of protein hydrolysate with a relative molecular mass distribution <1000 in the hydrolyzed protein is 34-70%, the peptide content (calculated on a dry basis) in the hydrolyzed protein is not less than 33 g / 100 g, and the total free amino acid content in the hydrolyzed protein is not less than 1 g / 100 g. The composition can effectively treat diarrhea and is suitable for people with gastrointestinal dysfunction and severe patients with diarrhea symptoms.

The invention discloses a hydrolysate deacidification-dehydration coupling process and a byproduct recovery method, and belongs to the technical field of hydrolysate treatment. The method comprises the following steps: pretreating a hydrolysate raw material through two-stage filtration; adding the pretreated hydrolysate, a deacidification agent and a dehydration auxiliary agent into a coupling reaction device, and synchronously carrying out deacidification-dehydration reaction under the conditions of 60-120 DEG C and 0.1-0.5 MPa; after the reaction, separating to obtain a deacidification and dehydration product and a mixed solid phase; and dissolving and crystallizing the mixed solid phase to recover high-purity byproducts. The deacidification and dehydration synergistic interaction is achieved, the acidity reduction rate is larger than or equal to 85%, the water content is smaller than or equal to 5%, and the process time consumption is shortened by 28.6%; the byproduct purity is not less than 90%, and the recovery rate is not less than 80 A tail gas treatment system is additionally arranged to ensure that environmental protection reaches the standard. The process is suitable for biomass, polysaccharide and protein hydrolysate treatment, has the advantages of high efficiency, good resource utilization rate and the like, and provides an efficient solution for hydrolysate industrial treatment.

Protein hydrolysate compositions and methods of making the same are disclosed. The protein hydrolysate composition has a protein-rich organic content. The protein hydrolysate composition may be substantially free of exogenous chelating agents, chaotropic agents and surfactants. The protein hydrolysate composition may be low in ash content. The protein hydrolysate composition is produced by processing a biomass, e.g., a microbial biomass, through a combination of physical, chemical and / or enzymatic treatments. The protein hydrolysate may be sourced via microbial biomass from CCk as a carbon source. Also disclosed are methods of using the protein hydrolysate compositions, e.g., as a biostimulant.

[Problem] To provide a matsutake primordium having a high methyl cinnamate content and a method capable of obtaining such a matsutake primordium having a high methyl cinnamate content at an artificially high yield. [Solution] Cells having the ability to form a primordium that have been isolated from matsutake fruiting bodies are cultured in a medium containing a protein degradation product and having a specific phenylalanine concentration.

The invention discloses a molecular docking screening method and application of hermetia illucens protein antioxidant peptides, and relates to the technical field of biologication.The method comprises the following steps that S1, hermetia illucens protein is prepared through enzymolysis, wherein hermetia illucens defatted protein powder serves as a raw material, enzymolysis is conducted through pepsin under the enzymolysis conditions that the pH is 2.0, the temperature is 40 DEG C, the enzyme adding amount is 3% of the protein mass, the enzymolysis time is 3 h, and enzymolysis liquid is obtained; after the reaction is finished, inactivating, centrifuging, collecting supernatant, and freeze-drying to obtain hermetia illucens protein hydrolysate; according to the molecular docking screening method and application of the hermetia illucens protein antioxidant peptide, the molecular docking technology is specifically applied to efficient screening of the hermetia illucens-sourced antioxidant peptide, KEAP1 is taken as a key target, high-activity candidate peptide is rapidly and accurately positioned from massive enzymolysis peptide fragments, and the screening efficiency of the hermetia illucens-sourced antioxidant peptide is improved. The defects that a traditional blind screening method is large in workload, long in period and high in cost are overcome, and the screening efficiency and the success rate are remarkably improved.

The invention relates to the technical field of food processing, in particular to an alkali-induced glycosylated tilapia protein hydrolysate loaded with fat-soluble polyphenol as well as a preparation method and application of the alkali-induced glycosylated tilapia protein hydrolysate. The glycosylated tilapia mossambica protein hydrolysate is prepared by adopting flavourzyme with mild action conditions for moderate hydrolysis and combining with a green, efficient and reaction degree-adjustable alkali-induced propylene glycol alginate (PGA) glycosylation strategy, so that the water solubility and emulsibility of tilapia mossambica protein are effectively improved, and the tilapia mossambica protein hydrolysate can be used for preparing the tilapia mossambica protein hydrolysate. The glycosylated protein hydrolysate (modified tilapia protein) is used as a carrier to develop an oral delivery system of fat-soluble polyphenols such as quercetin and the like, so that the bioaccessibility of the fat-soluble polyphenols is improved, and the fat-soluble polyphenols can be developed into functional food ingredients to improve the health condition of special groups; the high-value utilization of the tilapia protein in the protein-based polyphenol functional food ingredients is promoted.

The application discloses selenium-containing chickpea peptides, a preparation method thereof and application of the selenium-containing chickpea peptides in preparation of blood pressure lowering drugs, and belongs to the technical field of bioactive peptides. Selenium-enriched Bacillus natto is used to ferment chickpeas to obtain selenium-containing chickpea protein hydrolysate, and then fermentation conditions are optimized and quantitative peptidomics technology is used to obtain selenium-containing chickpea peptides PQM and PQML. Experiments prove that the selenium-containing chickpea peptides have good in-vivo stability, bioavailability and blood pressure lowering activity, have an effect of inhibiting angiotensin converting enzyme (ACE) activity, and have potential for preventing or treating hypertension.

Structured food compositions, such as meat analogue compositions, which include protein products from microorganisms, such as microorganism-derived protein hydrolysates, are described. Methods of making such products are also described.

The invention discloses a walnut source DPP-IV inhibitory peptide as well as a preparation and screening method and application thereof. The walnut source DPP-IV inhibitory peptide comprises FPAG and / or LPSYQPTP, the preparation and screening method comprises the following steps: (1) adding walnut protein powder into deionized water, and fully dissolving to obtain a walnut protein solution; (2) adding hydrolyzed compound protease into the walnut protein solution for enzymolysis to obtain walnut protein hydrolysate freeze-dried powder; (3) re-suspending the walnut protein hydrolysate freeze-dried powder in deionized water, and performing ultrafiltration separation by using an ultrafiltration membrane with the molecular weight cutoff of 1kDa to obtain a component F1; step (4), performing peptide fragment sequence identification on the obtained F1 component through peptidomics; and step (5), screening from peptide fragment sequences obtained by identification through a bioinformatics technology. The walnut source DPP-IV inhibitory peptide screened by the invention can be used for preparing a medicine or food with a DPP-IV inhibition function, so that the problems that the existing walnut source DPP-IV inhibitor has many side effects and the like are solved.

The present invention relates to a protein hydrolysate, wherein at least 90 wt.% of the peptides in the protein hydrolysate have a molecular mass of at most 500 Dalton, and wherein between 35 and 55 wt.% of the peptides have a molecular mass between 204 and 300 Dalton.

The invention discloses a method for inducing aroma enhancement of egg yolk hydrolysate by utilizing Maillard reaction, and belongs to the technical field of food processing. The method disclosed by the invention comprises the following steps: taking fresh egg yolk, homogenizing, adding water, adjusting the pH value to 7-8, respectively adding protease and cysteine endonuclease for reaction, and taking supernatant after the reaction, thereby obtaining the aroma-enhanced egg yolk protein hydrolysate. Then xylose is added, the initial pH is adjusted to 7.0-7.5, and homogenization treatment is performed; and carrying out spray drying on the homogenized system to obtain powder, placing the powder in a sealed container, and carrying out a dry heat reaction to obtain the aroma-enhanced egg yolk hydrolysate. The egg yolk hydrolysate is rich in characteristic volatile compounds such as furans, pyrazines, aldehydes and the like, presents rich toast bread fragrance, nut fragrance, soft meat fragrance and scorch fragrance, has excellent oxidation resistance, and improves flavor and optimizes taste experience through synergism of amino acid and nucleotide; the product is used as a natural flavor base material for food processing of seasonings, meat products, egg products and the like, the process is simple and controllable, and the method is suitable for industrial production.

The invention relates to an isolated peptide having an amino acid sequence selected from the group consisting of: Pro-Val-Leu-Lys (SEQ ID NO: 1); Pro-Leu-Pro-Arg (SEQ ID NO: 2); Glu-Ala-Glu-Phe-Asp (SEQ ID NO: 3); Pro-Glu-Arg-Asn-Glu (SEQ ID NO: 4); Pro-Gln-Pro-Glu-Arg (SEQ ID NO: 5); Glu-Phe-Asp-Glu-Lys-Pro-Ala-Asp (SEQ ID NO: 6); Glu-Asp-Ala-Pro-Val-Leu-His (SEQ ID NO: 7); Glu-Phe-Asp-Ala-Arg-Pro-Thr (SEQ ID NO: 8); Glu-Glu-Thr-Pro-Ser-His-Asp (SEQ ID NO: 9); Glu-Glu-Val-Glu-Glu-Glu-Glu-Val-Glu (SEQ ID NO: 10); Glu-Phe-Asp-Glu-Lys-Ala-Pro-Asp (SEQ ID NO: 11); Glu-Asn-Ala-Pro-Asp-Gln-Lys (SEQ ID NO: 12); Glu-Asp-Met-Ala-Pro (SEQ ID NO: 13); Pro-Ile-Asn-Asp-Asn (SEQ ID NO: 14); Pro-Thr-Asp-Leu (SEQ ID NO: 15); Pro-Val-Ala-Glu (SEQ ID NO: 16); Arg-Asn-Gly-Pro-Arg (SEQ ID NO: 17); Arg-Ala-Glu-Asp-Thr-Ala-Thr-Tyr-Tyr (SEQ ID NO: 18); Glu-Gly-Asp-Ser-Ala-Ala-Ile (SEQ ID NO: 19); Glu-Pro-Glu-Gly-Asp-Val-His-Gln (SEQ ID NO: 20); and Glu-Asp-Glu-Val-Leu-Ala-Thr-Pro (SEQ ID NO: 21), a protein hydrolysate comprising the isolated peptides, and use thereof as a taste-modulating agent in food and pharmaceutical preparations.

The application discloses a Chlorella protein hydrolysate-based oil gel and a preparation method and application thereof in the field of plant-based food, and the preparation method comprises the following steps: using or not using a protease to hydrolyze Chlorella protein to obtain Chlorella protein hydrolysate, and dispersing the Chlorella protein hydrolysate in water to form a Chlorella protein hydrolysate suspension; after using gluconolactone to induce the Chlorella protein hydrolysate suspension to form a hydrogel, diluting the hydrogel with water, and performing one-time high-speed shearing treatment to obtain a Chlorella protein hydrolysate microgel particle dispersion; adding the Chlorella protein hydrolysate microgel particle dispersion into liquid edible vegetable oil, and performing secondary high-speed shearing treatment to obtain a coarse emulsion; adding an edible polysaccharide solution into the coarse emulsion, and performing three-time high-speed shearing treatment to obtain a Chlorella protein hydrolysate-based oil-in-water emulsion; and removing water in the Chlorella protein hydrolysate-based oil-in-water emulsion to obtain the Chlorella protein hydrolysate-based oil gel. The Chlorella protein hydrolysate-based oil gel has good texture performance and oil retention.

This invention relates to an anti-hair loss composition, characterized by being a mixture of component A and component B; wherein component A comprises the following components: water, sodium fatty alcohol polyoxyethylene ether sulfate, CAB-35 surfactant, CMT-30B surfactant, ML35 foaming agent, G14S hair conditioning agent, trihydroxystearin, and plum fermentation liquid. Its advantages are that the composition is mild and non-irritating, effectively reducing hair loss and promoting hair growth. Its core relies on the protective effect of silk protein hydrolysate on keratin to prevent breakage and strengthen hair, while plum extract helps control oil and improve the hair growth environment. Simultaneously, the growth-promoting effect of plum extract on hair follicle cells enhances the anti-hair loss effect, while also nourishing the scalp, improving dry and frizzy hair, and enhancing hair smoothness and strength.

The present invention is enclosed in the area of animal feed, in particular in the field of fish feed. Aquaculture has emerged as a crucial sector in meeting the growing global demand for seafood. However, stress in aquaculture fish species can arise from numerous sources including handling, transportation, environmental fluctuations, and interactions with conspecifics. The object of the present invention is a feed comprising kyotorphin (KTP), preferably in its amidated derivative (Tyr-Arg.NH2) and a protein hydrolysate. The KTP is present in the feed by supplementation of analogues, or endogenously within as part of a dietary protein hydrolysate, as the dipeptide TyrArg. The purpose of the present invention is to provide a functional feed to mitigate stress responses in fish species used in aquaculture by enhancing stress tolerance, disease resistance, and growth efficiency in fish, ultimately leading to improved production outcomes and economic returns for aquaculture operations.

The invention provides a composition with the effects of assisting in lowering blood pressure, dissolving thrombus and softening blood vessels and a preparation method thereof. The composition is prepared from muskmelon powder, a white kidney bean extract, konjaku flour, coffee powder, inulin, psyllium husk powder, chia seed powder, earthworm protein hydrolysate, a folium cortex eucommiae extract, natto freeze-dried powder, raw carthamus tinctorius skin and auxiliary materials according to a specific weight percentage. Core active components such as a white kidney bean extract, an earthworm protein hydrolysate, natto freeze-dried powder and a folium cortex eucommiae extract are screened, and the activity and the absorption rate of the components are improved through optimized enzymolysis, ultrafiltration, precise freeze-drying and other preparation processes; all the components cooperate with one another to reduce blood pressure, resist oxidation and protect blood vessels, an active component network with a multi-target synergistic effect is formed, a mixing process is improved, low-sugar auxiliary materials and convenient dosage forms are matched, thermosensitive components are prevented from being inactivated, and the composition is suitable for people suffering from diabetes and hypertension.

The invention relates to the technical field of gene drugs, in particular to buffalo milk protein peptide as well as a preparation method and application thereof, and mainly aims at determining the optimal hydrolysis condition for extracting antidiabetic protein hydrolysate from buffalo milk. In the hydrolysis process, several milk source raw materials of buffalo milk, namely freeze-dried skimmed milk, casein, whey protein and skimmed milk, are used, then the several raw materials are subjected to enzymolysis, and pepsin, alkaline protease and papain are respectively used as three enzymes for enzymolysis; by researching the optimal enzymolysis conditions, further analyzing enzymolysis products with alpha-glucosidase inhibition and recovering the enzymolysis products, several six protein peptides with alpha-glucosidase inhibition characteristics are obtained, and through protein structure analysis, the protein peptides have the alpha-glucosidase inhibition characteristics, have a good blood sugar reduction function and can be used for preparing the blood sugar-reducing peptide with the alpha-glucosidase inhibition characteristics. And a new raw material is provided for later preparation of hypoglycemic drugs or foods.

The invention provides a protein raw material pretreatment method and application, and belongs to the technical field of protein raw material treatment.The protein raw material pretreatment method comprises the steps that a high-fiber protein raw material is prepared and subjected to pulsed electric field PEF treatment, stirring or circulation is conducted in the treatment process to guarantee the treatment uniformity, and after electroporation is generated, the protein raw material subjected to PEF treatment is obtained; the method comprises the following steps: performing PEF treatment on a protein raw material, adjusting the solid-liquid ratio of the protein raw material subjected to PEF treatment, sequentially performing sequential gradient enzymolysis, controlling the peptide chain length by monitoring the enzymolysis degree to obtain a protein hydrolysate with a specific peptide chain length, performing amyloid fibril treatment on the protein hydrolysate, and performing low-temperature drying on the treated product to finally obtain the functional protein raw material. According to the method, the pretreatment uniformity and the enzymolysis efficiency of the protein raw materials can be improved, the peptide chain length is accurately controlled, the protein digestibility is improved, the anti-nutritional factor content is reduced, and the functional protein raw materials suitable for livestock and poultry and aquatic feed production are produced.

A composition, preferably a cosmetic composition, comprising - a rapeseed protein hydrolysate comprising protein fragments, wherein at least 50% (w / w) of the protein fragments has a molecular weight of less than 3.5 kDa; and - optionally a cosmetically acceptable further ingredient.

To provide alternative protein-rich food products that are not derived from animals, but are free of unhealthy meat-related components such as saturated fatty acids and cholesterol, are free of the deleterious environmental effects of animal husbandry, and provide similar texture and flavor characteristics and functional properties as animal meat.SOLUTION: Structured food compositions, such as meat substitute compositions, comprising microbial-derived protein products, such as microbial-derived protein hydrolysates, are provided. Methods of making such products are also provided. Also provided are flavor potentiator compositions comprising heme-containing polypeptides, such as microbial-derived flavohemoglobin, and food compositions, such as meat substitute compositions, comprising the heme-containing flavor potentiator compositions. In one aspect, the flavor potentiator comprises a heme-containing polypeptide from a hydrogen-oxidizing microorganism, wherein (i) the hydrogen-oxidizing microorganism is a Cupriavidus microorganism or (ii) the hydrogen-oxidizing microorganism grows under microaerobic conditions.SELECTED DRAWING: None

To provide a composition that can improve the quality of sleep. [Solution] A composition for improving sleep quality containing globin protein hydrolysate.

The present invention relates to a homogeneous fertilizer particle, comprising one, two or three from 1.0 to 30.0 wt.% of nitrogen (N), from 1.0 to 52.0 wt.% of phosphorus (P) (expressed as P2O5), from 1.0 to 60.0 wt.% of potassium (K) (expressed as K2O), depending on whether the fertilizer particle is a K, a NP or a PK, or a NPK fertilizer, and from 0.0001 to 5.0 weight% of one or more protein hydrolysates as a biostimulant, based on the total weight of the fertilizer particle. The present invention furthermore relates to a method to produce such fertilizer particles.

According to the invention, a preparation is described which contains at least one calcium compound selected from the group consisting of calcium phosphates, calcium fluorides and calcium fluorophosphates and hydroxyl derivatives and carbonate derivatives of these calcium salts, calcium hydroxides and calcium oxides precipitated using at least one protein component selected from proteins and protein hydrolysates, and at least one crosslinking agent for the protein component and / or non-set cement.

The invention belongs to the technical field of bioactive protein peptides / protein hydrolysates, and particularly relates to an anti-aging soybean protein peptide and a preparation method thereof. Soybean protein is used as a raw material, the soybean protein is firstly subjected to thermal denaturation treatment to change the secondary structure of the soybean protein, and then the alkaline subunit amount of the thermal denaturation soybean protein is increased by utilizing high static pressure physical treatment, so that the exposure of hydrophobic groups in soybean protein molecules and the increase of enzyme action sites are facilitated, and the proteolysis rate is further increased. And finally, carrying out enzymolysis on the treated soybean protein by using alkaline protease to obtain the soybean protein peptide. The soybean protein peptide obtained by the preparation method disclosed by the invention has very strong antioxidant activity and a good anti-aging effect.

The invention discloses walnut undecapeptide as well as a preparation method and application thereof. The amino acid sequence of the walnut undecapeptide is as shown in SEQ ID NO. 1. The walnut undecapeptide has good immunoregulatory activity, and can be applied to preparation of immunoregulatory active drugs, food or health care products. The method comprises the following steps: carrying out protease enzymolysis on a walnut protein aqueous solution, carrying out solid-liquid separation after enzyme deactivation, carrying out membrane separation on the obtained supernatant, and drying to obtain a walnut protein hydrolysate; and purifying active peptides from the walnut protein hydrolysate by using a reversed-phase chromatographic column, collecting target active peptide components eluted by ethanol with a volume fraction of 7%, concentrating, purifying by using a sephadex chromatographic column, concentrating, and drying to obtain the walnut undecapeptide. The preparation method is of great significance in promoting deep processing and utilization of walnut protein, increasing the added value of products and promoting sustainable development of the industry.

The invention belongs to the technical field of coarse cereal instant porridge, and discloses a processing technology of fermented coarse cereal instant porridge, which comprises the following steps: S1, raw material mixing and enzymolysis: adding lactic acid / beta-cyclodextrin / monoglyceride composite treatment liquid into coarse cereals such as oats and the like, performing ultrasonic-microwave treatment, performing enzymolysis by using lectinase / beta-glucanase composite enzyme liquid, and separating to obtain coarse cereal solid particles and filtrate; preparing wild rice chitosan microspheres from the filtrate; s2, fermentation: firstly preparing a quinoa bran fiber-quinoa protein hydrolysate-Arabic gum-wild rice chitosan microsphere coarse cereal source composite skeleton carrier, then adding highland barley vinasse extract and the like into coarse cereal solid particles, and inoculating composite strains for fermentation; s3, pre-crosslinking coating and sterilization: pre-crosslinking phospholipid composite grafted polysaccharide and oat beta-glucan oligosaccharide to obtain a coating solution, adding a buckwheat flavone / grape seed polyphenol compound, mixing with the fermented particles and the like, performing spray drying, and sterilizing to obtain a finished product. The process solves the problems of low dissolution rate, component antagonism and easy caking during normal-temperature cold water brewing of the existing coarse cereal instant porridge.

The present invention relates to a process that, by using as raw materials by-products from the agribusiness industry, such as waste biomass from the processing of food flours, enables chemical intermediates and protein hydrolysates yielding amino acids with biostimulant action for plants to be produced from the polysaccharides and proteins present in such biomass. The process comprises sugar release by enzymes, fermentation, ethanol separation and protein hydrolysis by enzymes including solid-liquid separations at different points of the procedure and the optional release of arabinoxylans.