693 results about "Protein hydrolysates" patented technology

Disclosed are concentrated, liquid, human milk fortifier compositions comprising from about 15% to about 45% by weight of protein, on a dry weight basis, and having a caloric density of from about 1.25 kcauml to about 6.0 kcau / ml, wherein the liquid human milk fortifier composition is added to human milk in a volume-volume ratio of from about 1:3 to about 1:9. These composition include embodiments comprising carbohydrate and fat, that are formulated with improved stability by selecting any one of the following variations: 1) certain whey-casein protein blends, 2) water insoluble calcium-containing materials, 3) protein hydrolysates, and 4) aseptically packaged concentrates. The liquid concentrates are especially useful for providing nutrition to preterm infants in neonatal intensive care units or similar other institutional setting, and to minimize the risk of introducing microbial contaminants such as Enterobacter sakazakii to infant feedings during preparation in such institutional settings.

The present invention describes protein hydrolysates: obtainable by the hydrolysis of a protein containing substrate; comprising free amino acids and peptides; and wherein the molar fraction of at least one free amino acid, present in the protein hydrolysate is at least a factor 2.5, preferably at least a factor 3, more preferably at least a factor 3.5 times higher than in a hydrolysate of the same protein containing substrate which has been completely hydrolysed to free amino acids, wherein the molar fraction of the at least one free amino acid in the protein hydrolysate is at least 25%; and wherein the Amino Acid Quotient (AAQ) in the protein hydrolysate is at least 10%. These protein hydrolysates can be used in the preparation of food compositions, wherein these protein hydrolysates provide for novel and unexpected flavours. Moreover these protein hydrolysates are applicable in personal care applications.

A process for making a surfactant composition involving: (a) providing a starting mixture containing: (i) an aqueous alkali solution; (ii) at least one amino acid and / or a salt thereof; (iii) a fatty acid chloride; (iv) an acylatable surfactant precursor selected from the group consisting of a protein hydrolyzate, a polyamino acid, an aminosulfonic acid, an amino sugar, a nonionic surfactant, and mixtures thereof; and (v) up to about 15% by weight, based on the weight of the starting mixture, of a polyol component; (b) providing a stirring mechanism; and (c) reacting (ii) and (iii), with stirring, to form the surfactant composition.

The present invention provides protein hydrolysate compositions, processes for making protein hydrolysate compositions, and food products comprising protein hydrolysate compositions. The protein hydrolysate compositions generally comprise polypeptide fragments having primarily either an arginine residue or a lysine residue at each carboxyl terminus.

The present invention includes compositions and drying methods for preserving sensitive bioactive materials, such as peptides, proteins, hormones, nucleic acids, antibodies, drugs vaccines, yeast, bacteria (probiotic or otherwise), viruses and / or cell suspensions, in storage. The compositions include a carbohydrates component and a glass enhancer component, wherein the carbohydrate component includes a mixture of di-, oligo- and polysaccharides and the glass enhancer includes ions of organic acid and protein hydrolysates. The composition is prepared by dispersing all the solid components in a solution and then snap-frozen to form small beads, strings or droplets. The preferred drying method of the frozen beads, strings or droplets is initiated by a short purging and structure stabilizing step of the frozen particles under a vacuum pressure of less than <2000 mTORR followed by a primary drying step under vacuum pressure of more than >2000 mTORR and at a desired temperature. During the secondary and final drying step of the material a full vacuum pressure and elevated temperature are applied, to achieve a final desirable water activity of the dry material.

The invention relates to the culture medium research and development technical field of modern biological technology and provides a serum-free medium for MDCK cell large-scale adherent culture and single-cell suspension culture, which comprises 21 amino acids, 6 vitamins, 8 salts, 8 lipids, 4 trace elements, 2 buffers, 1 protein hydrolysate, 1 acid-base indicator and 6 other additives. The serum-free medium can be prepared by the conventional preparation method, and an application method thereof is the conventional method. The serum-free medium has the beneficial effects that: the serum-free medium does not contain serum, has clear components, is beneficial for separating and purifying the product and improves the product quality; the serum-free medium supports long-term subculture of MDCK cells and does not require long-term and complex adaptation process; and the serum-free medium can well support the adherent growth and single-cell suspension growth of the MDCK cells, has clear components and easy preparation and utilization, and is suitable for mass production of biological products.

The invention relates to a method for enzymatically obtaining protein hydrolysates for human consumption, animal feed and cosmetics. The process involves the use of a proteolytic composition derived from fish, such as Cod (Gadus morhua), to obtain hydrolysates which have a non-bitter taste and retain the flavor and aroma of the protein-containing material which is hydrolyzed: e.g. when hydrolyzing protein-containing material from marine organisms or parts thereof, such as fish, shrimp, lobster or other seafood according to the invention, a protein hydrolysate is produced that has a characteristic natural flavor of the organism Also provided are food products comprising the hydrolysates of the invention, such as soup, sauce, cheese, HVP, meat extract and flavoring agent, broth, paté, mousse, frying dough, orly dough, and pastries.

This invention relates to a process for the production of a cultured savory base by hydrolyzing a protein-containing material using a combination of at least one enzyme with at least one thermotolerant lactic acid bacteria strain selected for its ability to provide a glutaminase activity. The invention also relates to an isolated strain of thermotolerant lactic acid bacteria strains selected for their ability to provide a glutaminase activity and their use for preparing a seasoning or flavor for culinary or petfood products.

The invention relates to a clean chitosan production technology and relates to the technical field of comprehensive utilization of wastes generated in a process of aquatic product processing and treatment and comprehensive utilization of food processing wastewater. The clean chitosan production technology comprises the following steps of: carrying out instant high-pressure steam explosion pretreatment on shrimps and crab shells taken as raw materials through a high-density steam explosion device, dissolving and extracting most of protein in the raw materials through enzymatic hydrolysis to prepare food-grade protein hydrolysate; sequentially soaking enzymolysis residues in NaOH and hydrochloric acid to prepare the high-quality chitosan; sequentially carrying out filtration treatment on an alkali waste liquid through a security filter, a carbon carbide ultrafiltration membrane and an alkali-resisting NF (Nanofiltration) membrane to obtain feed-grade protein and recycled dilute alkali liquid; treating the waste liquid soaked in acid through the security filter and the carbon carbide ultrafiltration membrane to obtain the neutral CaC12 clear water which is discharged after reaching the standard. Compared with the prior art, the clean production technology has the advantages that the whole new technology is clean and environmentally-friendly, a seperation and purification technology is simple, reasonable, short in working procedures and convenient in operation, simultaneously resources are fully and comprehensively utilized, secondary pollution is avoided, economic benefits are obviously improved, and industrialized application value is fulfilled.

The invention provides a novel fourth-generation infant formula. The novel fourth-generation infant formula comprises the following ingredients: lactose, desalted whey powder, 1,3-dioleoyl 2-palmitoyl triglyceride, hydrolyzed whey protein, fructo-oligosaccharide, galactooligosaccharide, mineral substance premix compound, casein hydrolysate, whey protein concentrate, soyabean lecithin, vitamin premix, lactoferrin, L-tyrosine, nucleotide, L-tryptophan, L-methionine and the like. The infant formula is added with appropriate amount of 1,3-dioleoyl 2-palmitoyl triglyceride, appropriate amount of protein hydrolysate, monomer amino acid and the like, mineral contents such as phosphorus, sodium and the like in milk powder are reduced, therefore, the fat structure, the amino acid composition, the amino acid content and the osmotic pressure in the milk powder are close to those of the breast milk, the infant formula is balanced and comprehensive in nutrition and is appropriate in composition, all substances have synergistic effect, the bioavailability of protein and the fat is effectively improved, and the infant formula can promote digestive absorption and skeletal development of babies and can reduce the visceral organ burden.

The invention discloses a method for extracting oil from peony seeds or peony seed dregs. The method comprises the following steps: (1) pretreatment; (2) enzymolysis; (3) centrifuging; (4) demulsification; (5) dreg washing. Free oil, an emulsion layer, protein hydrolysate and dregs are obtained after enzymatic hydrolysate is centrifuged, wherein the free oil is peony seed oil, and a certain amount of oil can also be recovered after the emulsion layer and the dregs are subjected to demulsification and dreg washing respectively. According to the method, the peony seed oil can be extracted without drying raw materials, the operation is simple, the requirement for equipment is low, the oil yield is high, the oil quality is good, solvent residues and environmental pollution are avoided, and the obtained protein hydrolysate and dregs can also be further used comprehensively.

The invention relates to a method for preparing a meat flavour essence through solid state fermentation, and the method comprises the following steps of: removing impurities contained in bean pulp, smashing the bean pulp, then adding auxiliary materials, uniformly mixing, then cooking, and cooling; inoculating microbes, then fermenting, adding fresh meat, salty water and protease, uniformly mixing, carrying out enzymolysis and enzyme deactivation, and cooling to room temperature to obtain enzymatic hydrolysate; and adding reducing sugar, amino acid and thiamine to the enzymatic hydrolysate, uniformly mixing, and carrying out maillard reaction at 90-120 DEG C for 1-4 hours to obtain the meat flavour essence. The meat flavour essence prepared through the method disclosed by the invention has the advantages of rich meat flavour, full taste and lasting after-taste and certain nutritional health-care action; and the method disclosed by the invention can be used for preparing the protein hydrolysate by partially or completely substituting a protease preparation generated in the fermentation process of the microbes for a commercial enzyme preparation, thereby saving a large quantity of expensive enzyme preparations and reducing the production cost.

An oral and dental hygiene product is comprised of a composite material comprising calcium salts which are slightly soluble in water, in the form of nanoparticulate primary particles having a length of from 5 to 150 nm and a cross section of from 2 to 50 nm and protein components selected from the group consisting of proteins, protein hydrolysates and protein hydrolysate derivatives, and 0.1 to 9% by weight of a cleaning agent based on the total weight of the product.

Disclosed is a composition comprising a combination of: Polygonum fagopyrum seed extract, Chlorella vulgaris extract, palmitoyl wheat protein hydrolysate, algae extract, and tripeptide. The composition may further comprise one or more of the following: Citrus unshiu peel extract, Sphacelaria scoparia extract, Bambusa vulgaris extract, Pisum sativum (pea) extract, Evodia rutaecarpa fruit extract, and dipalmitoylhydroxy proline. Such compositions are useful in methods for aiding in improving skin firmness, lifting the skin, preventing or decreasing skin sagging, and preventing or decreasing visible signs of aging resulting from internal and external causes such as environmental stresses.

The invention belongs to the technical field of biomedicine, and relates to a selenium-enriched ground beetle breeding method, capsules of freeze-dried powder of the selenium-enriched ground beetles, protein hydrolysate of the selenium-enriched ground beetles, and medicinal uses of the selenium-enriched ground beetles, which solve the technical problems of poor technical medicinal effect and the like. The breeding method includes: A, building a breeding site; B, preparing breeding soil; C, preparing feed; D, determining breeding density; and E, controlling disease and insect damage. The selenium-enriched ground beetles bred by the selenium-enriched ground beetle breeding method can be made into the freeze-dried powder capsules and protein hydrolysate. The invention has the advantages that the method is easy to implement, the bred ground beetles are rich in beneficial components necessary for human bodies, especially selenium and zinc, and the ground beetles are high in medicinal value.

The invention discloses a dietetic food composition for losing weight. The composition comprises the following ingredients: fresh meat and aquatic product, vegetables, fruits, dried mushroom products, cereals, beans, nut meat, edible vegetable oil, eggs, protein hydrolysate powder, dietary fiber powder, konjac powder, guar gum, polyols, phosphatidylcholine, fruit polyphenols, L-carnitine, vitamin A, vitamin E, vitamin C, thiamine hydrochloride, riboflavin, nicotinamide, pyridoxine hydrochloride, folic acid, cyanide-containing cobalt and pantothenic acid. By adopting the dietetic food composition disclosed by the invention, the total energy intake is reduced under the premise of ensuring normal basic metabolic rate, the requirements of the body on protein at a low-energy diet period are improved, and related nutrients are replenished to facilitate body fat, visceral fat and blood lipid metabolism, so as to achieve the target of losing weight. Meanwhile, the invention also discloses a preparation method of the dietetic food composition and use of the dietetic food composition in preparation of a medicament for preventing or treating an obesity disease.

The invention relates to a scalp nursing solution and a preparation method thereof. The scalp nursing solution is prepared from the following components in percentage by weight: 0.5-3 percent of vitamin B complex, 0.5-5 percent of bionic phospholipid, 0-5 percent of dihydroxypropyl PEG-5 linoleammonium chloride, 0.1-10 percent of conditioner, 2-20 percent of modified ethanol and the balance of deionized water, wherein the vitamin B complex consists of vitamin B6 and vitamin B3; the conditioner is one or more of water soluble plant oil, humectant, siloxane emulsion and protein hydrolysate. After the scalp nursing solution is used for multiple times, hair can be smooth and is not greasy or easily dirty, hair loss is prevented, damaged hair can be remarkably repaired, the scalp is not irritated, moisture can be maintained, and oil can be controlled. The scalp nursing solution has a prominent effect of removing dandruff and relieving itching.

The present invention relates to a kind of brain protein hydrolysate and the production process of its freeze dried preparation. The brain protein hydrolysate for injection is prepared with pig brain and through the steps of homogenating in a colloid mill to collect slurry, hydrolyzing with pepsase and pancreatin to collect supernatant, filtering with filer paper to collect filtrate, separating and purifying the filtrate with hydroxyapetite column, regulating peptide map and collecting object, ultrafiltering with membrane of intercepting molecular weight 8 KD to collecting filtrate, nitrogen and amino acid analysis and adding amino acid in the required amount, and fine filtering with 0.22 micron filtering membrane. The brain protein hydrolysate for injection may be further freeze dried to obtain freeze dried brain protein hydrolysate preparation. The production process is environment friendly, high in yield and low in cost.

The invention relates to a method for preparing bonito stick protein hydrolysate with an effect of reducing uric acid. The method comprises the following main steps of raw material heat treatment, restriction digestion, membrane separation-anion exchange chromatography-gel filtration chromatography separation, concentration and spray drying so as to obtain the bonito stick protein hydrolysate with an effect of reducing uric acid. The amino acid analysis indicates that the zymolyte peptide fragment primary amino acid sequence contains four amino acids, namely histidine, arginine, lysine and threonine, the total mass content of the four amino acids is 70 percent of the total amino acid content of zymolyte. MALDI-TOF-MS mass spectrum determines that the molecular weight of the main peptide effective ingredient is less than 700Da. In-vitro uric acid reduction experiments prove that the bonito stick protein hydrolysate has a remarkable effect of inhibiting generation of uric acid, and has an inhibition rate over 50 percent; an oteracil potassium molded hyperuricemic rat animal model indicates that the bonito stick protein hydrolysate can be used for remarkably reducing the level of serum uric acid and serum creatinine of rats, and shows a relatively good kidney protecting effect.

The present invention describes a protein hydrolysate which is rich in tripeptides whereby the tripeptides are rich in proline at one end of the peptide.

The present invention relates to a setting retarder for compounds that set hydraulically, containing at least one at least simple adduct and / or condensate, the retarder being produced by the reaction of at least one protein hydrolysate, one pure amino acid, amino acid mixture, and / or the hydrochlorides thereof having at least one mono-, di-, oligo- and / or polycarboxylic acid which is not derived from an amino acid, and / or a carboxylic acid derivative derived therefrom, wherein the carboxylic acid is selected from a group including carboxylic acid anhydrides, carboxylic acid halogenides, and / or carboxylic acid active esters.

The invention discloses an ecological environmental protection fish protein liquid fertilizer. The ecological environmental protection fish protein liquid fertilizer comprises the following components in parts by weight: 1 to 50 parts of fish and shrimp protein hydrolysate, 1 to 50 parts of seaweed hydrolysate, 1 to 20 parts of molasses, 1 to 20 parts of urea, 1 to 20 parts of ammonium sulfate, 1 to 20 parts of potassium dihydrogen phosphate, 1 to 5 parts of zinc sulfate, 1 to 5 parts of boric acid, 1 to 5 parts of magnesium sulfate, 1 to 5 parts of ferrous sulfate, 0.5 to 2 part of manganese sulfate, 0.5 to 2 parts of copper sulfate and 0.1 to 1 part of sodium molybdate. According to the invention, waste fish sauce, shrimp heads, shrimp shells and seaweeds in processing of aquatic products are biologically treated, and converted into bioactive peptides, amino acids, seaweed polysaccharides and alginate nutrients which are easily absorbed by plants, and inorganic salts are added for manufacturing the ecological environmental protection fish protein liquid fertilizer for agricultural planting.

The present invention relates to the development and utilization of plant protein. Defatted wheat plumule as material is protein extracted and enzyme hydrolyzed to obtain hydrolysate containing several kinds of powerful ACE inhibiting active peptides. The obtained wheat plumule protein hydrolysate is one kind of polypeptide mixture containing Ala-Met-Tyr tripeptide, Val-Trp depeptide and other polypeptide compounds. The wheat plumule protein hydrolysate can lower blood pressure of hypertension patient by means of suppressing ACE, and is one safe and effective blood pressure lowering material. In addition, the hydrolysate has also effects of raising immunity and resisting oxidation.

The present invention provides a process for the preparation of protein hydrolysate from milk protein by treating milk protein with fungal protease at a pH of 7.5-8.5, a temperature of 40±5° C. for a time period of 30 min to 2 hours, followed by heating at 65-70° C. for at least 3 min, separating the clarified supernatant by a known manner and drying the clarified liquor thus obtained to get the protein hydrolysate.

The brain protein hydrolysate injection is prepared with health swine brain and contains 16 kinds of free amino acids and small amount of peptides. It is used in treating sequelae of craniocerebral trauma and cerebrovascular disease, improving cerebral function, etc. It is prepared through washing, homogenizing, thermal changing, enzyme hydrolysis, freezing, ultrafiltering, sterilizing and other steps.

The invention provides a method of making mangel immune polysaccharides and yeasts extract, belonging to the technique field of preparing biological products, such as polysaccharides, by yeasts. Said method includes the following steps: brewers' yeast residue bitter-removing, foreign substance-removing, brewers' yeast autolysis, brewers' yeast complex enzymolysis and deactivation, segregation of polysaccharides and protein hydrolysate liquid, blink spray drying of mangel immune polysaccharides and yeasts extract, etc; it is characterized on: high yield, good quality, low cost, and could achieve the aim of preparing mangel immune polysaccharides and yeasts extract at the same time from identical yeast material, enhance the availability ratio of yeasts, avoid pollution to the environment in traditional method as a result of simply preparing one certain product and discharging other assistant liquid.

The present invention provides protein hydrolysate compositions, processes for making protein hydrolysate compositions, and food products comprising protein hydrolysate compositions. The protein hydrolysate compositions generally comprise a mixture of oligopeptides having an average molecular size of less than about 10,000 Daltons, wherein at least about 60% of the oligopeptides are soluble at a pH of less than about 7.0.

The invention relates to a method for preparing brain protein hydrolysate for injection and a preparation thereof. The brain protein hydrolysate is prepared from pig brain tissues passing quarantine through a specific controlled enzyme hydrolysis technique, and active ingredients of the prepared brain protein hydrolysate comprise polypeptide with a molecular weight of less than 10,000 Daltons and 16 free amino acids which are hydrolyzed amino acids; each milligram of total nitrogen of the obtained brain protein hydrolysate contains 4.68 to 7.02 milligrams of amino acids; the preparation method comprises the processes of homogenization, primary enzymolysis, inactivation, secondary enzymolysis, cation exchange column adsorption, desorption, blending, ultra-filtration, preparation and the like; when the obtained brain protein hydrolysate is used for preparing the preparation, an amino acid does not need adding; and pharmacological experiment results show that the prepared preparation has good treatment effect and good safety.

An iron-protein hydrolysate complex which may be used to fortify foods and beverages with iron. The complex is formed of ferrous ions chelated to partially hydrolyzed egg white protein. The hydrolyzed egg white protein has a molecular weight in the range of about 500 to about 10,000. The complexes are sufficiently stable as to be suitable for use in sterilized products, such as retorted products. Moreover, despite the stability, the iron in the complexes has substantially the same bioavailability as ferrous sulfate.

The invention discloses a culture medium applicable to suspension and magnification cultivation of Vero cell microcarriers. The culture medium comprises amino acid, inorganic salt, vitamins, protein hydrolysate, lipid, buffer components and additives. The culture medium not only is applicable to static cultivation in a Vero square bottle and cultivation in a roller bottle, but also is supportive for suspension cultivation of the microcarriers and magnification cultivation of the microcarriers in a reactor, and can be used as a serum-free virus maintenance medium to realize serum-free virus production.