62 results about "Roller Bottle" patented technology

Methods for the maximizing parameter of the in vitro growth and expansion of mammalian cells, specifically postpartum-derived cells in containers such as roller bottles is described. Methods of optimizing growth rate and cell yield in such culture systems are provided. The methods are particularly adapted for human postpartum-derived cells, such as umbilicus-derived cells.

PCT No. PCT / GB95 / 01833 Sec. 371 Date Feb. 12, 1997 Sec. 102(e) Date Feb. 12, 1997 PCT Filed Aug. 2, 1995 PCT Pub. No. WO96 / 05285 PCT Pub. Date Feb. 22, 1996A cell culture apparatus is disclosed. The cell culture apparatus includes a chassis or supporting frame. The cell culture apparatus also includes a rotor releasably housing a plurality of cell culture vessels / roller bottles, the rotor being mounted about a substantially horizontal axis or shaft supported by said frame, with means provided to facilitate rotation of the rotor at a controlled speed. The cell culture apparatus also includes a reversible multi-channel pump or pumps, mounted on and rotating with the rotor. The cell culture apparatus also includes a manifold with one or more sealable external connections and a plurality of connections communicating with the individual channels of the multi-channel pump or pumps, each of the cell culture vessels being equipped with a microporous air vent to atmosphere and a dip tube. The dip tube is fixed with respect to the cell culture vessel and positioned to permit extraction of fluid when the cell culture vessel is stopped in a specific orientation. The dip tube of each vessel is individually connected to one channel of said multi-channel pump or pumps. The arrangement of the parts is such that the assembly comprising the rotor, cell culture vessels, pump, manifold and the connections to the cell culture vessels are rotatable about the horizontal axis. The arrangement also allows fluid to be injected into or extracted from the roller bottles simultaneously via a single external connection, under the influence of the multi-channel pump or pumps.

The invention provides an advanced roller bottle system for cell culture that efficiently, continually, and automatically replenishes spent media with fresh media. The roller bottle system optimizes media use by removing spent media in response to a predetermined condition change and replenishing the spent media with fresh.

A roller bottle is provided with a bottom wall, a top wall and a cylindrical side wall extending between the bottom and top walls. The top wall includes an opening to provide access to the interior of the roller bottle. The cylindrical side wall of the roller bottle is formed with at least one spiral pleat extending substantially from the bottom to the top for increasing cell growth surface area and for facilitating the flow of liquid to all interior surface areas of the bottle as the bottle is rolled about its longitudinal axis.

The invention provides a preparation method of a PCV2, PRRSV and mycoplasma hyopneumoniae triple inactivated vaccine. The technical scheme is that a brand-new preparation method is designed from culture, inactivation, complex and other levels based on growth characteristics of three microorganisms and physicochemical characteristics of an antigen substance. In the method, the PCV2 and the PRRSV are prepared by adopting a bioreactor mode; in the preparation process of an antigen, a pH value of fermented liquid is strictly controlled, so that the immunogenicity of a virus is effectively ensured;mycoplasam suis produces a strain in a bacteria fermenting tank; and in the breeding process of the strain, the homogenization of an intra-batch bacterial solution is ensured by setting dissolved oxygen, the pH value, rotating speed and the like, the defects of complex operation, high working intensity, easiness in pollution and the like in the rolling bottle culture process are eliminated, and the quality stability of the mycoplasam suis inactivated vaccine is effectively improved. Experiments show that the protection rates of the prepared triple inactivated vaccine for the PCV2, the PRRSV and the mycoplasma hyopneumoniae all exceed 80 percent, and preventing and treating demands on three pathogens are met.

The invention discloses a jellyfish disc culture device and a method for using the same. The structure includes a culture bottle and an electric roller bottle machine. Both ends are detachably connected to the side cover, and there is a hole in the middle of the side cover, and hemispherical depressions are evenly arranged on the bottle body. During the specific experiment process, the dish can do floating movement with the low-speed and effective water flow inside the culture bottle , The cylindrical bottle body has no dead ends and inflatable tubes, which effectively avoids individual damage, and at the same time, the water body does not produce air bubbles, which can effectively prevent the bubbles from entering the gastric cavity of the disc-shaped body and rise up, reducing the mortality rate. The survival rate of the jellyfish dish is effectively improved, and the accuracy of the indoor research experiment is guaranteed. It is conducive to the in-depth study of disastrous and economic jellyfish, and has a wide range of uses in related research fields such as fishery resource utilization and ecological restoration.

The invention discloses a culture medium applicable to suspension and magnification cultivation of Vero cell microcarriers. The culture medium comprises amino acid, inorganic salt, vitamins, protein hydrolysate, lipid, buffer components and additives. The culture medium not only is applicable to static cultivation in a Vero square bottle and cultivation in a roller bottle, but also is supportive for suspension cultivation of the microcarriers and magnification cultivation of the microcarriers in a reactor, and can be used as a serum-free virus maintenance medium to realize serum-free virus production.

Methods for the maximizing parameter of the in vitro growth and expansion of mammalian cells, specifically postpartum-derived cells in containers such as roller bottles is described. Methods of optimizing growth rate and cell yield in such culture systems are provided. The methods are particularly adapted for human postpartum-derived cells, such as umbilicus-derived cells.

The invention relates to a monkey embryo kidney epithelial cell Marc‑145 suspension adaptation strain and its application in cultivating PRRS virus and producing PRRS virus vaccine, belonging to the field of biotechnology. The present invention belongs to a disclosed monkey embryonic kidney epithelial cell Marc‑145 suspension adaptation strain, which is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: C201542. The invention also discloses the use of the Marc‑145 cell suspension adaptation Strain culture method of PRRS virus. The Marc-145 cell suspension adaptation strain provided by the present invention has realized the full suspension culture of Marc-145 cells in the culture medium, thereby adopting the Marc-145 cell suspension adaptation strain of the present invention to produce PRRS virus, easy to use in industrial production The bioreactor is amplified step by step to realize mass production of PRRS vaccines. Compared with the existing spinning bottle and carrier suspension culture technology, the present invention simplifies the production process, shortens the production cycle, reduces production costs, and further improves PRRS vaccine production. quality and production of vaccines against the disease.