81 results about "HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID" patented technology

Provided is a method for preparing a nano anticancer probe of a zirconium-porphyrin metal organic framework material. Anhydrous zirconium chloride, meso-4-carboxyphenyl porphyrin, cetyl trimethyl ammonium bromide, benzoic acid and polyethylene glycol are added into N,N-dimethylformamide for ultrasonic dissolving, and an obtained mixed solution is subjected to a heating reaction till purple sediment is precipitated out at the bottom; after centrifugal separation, the sediment is washed with N,N-dimethylformamide, ethyl alcohol and water, and freeze drying is carried out to obtain a nano anticancer probe 1; chemotherapeutic medicine is dissolved in a 4-hydroxyethylpiperazine ethane sulfonic acid buffer solution, the nano anticancer probe 1 is added, centrifugal separation is carried out after stirring is carried out at normal temperature, washing is carried with a 4-hydroxyethylpiperazine ethane sulfonic acid buffer solution, freeze drying is carried out, and a nano anticancer probe 2 is obtained. The method has the advantages that the process is simple, and implementation is easy; the nano anticancer probe is used as an imaging induced synergic treatment system in the field of biomedicine, and meanwhile cancer is detected and treated.

The invention discloses a whitening and spot-removing facial mask containing coenzyme Q10, and a preparation method of the whitening and spot-removing facial mask. The whitening and spot-removing facial mask containing coenzyme Q10 comprises the following ingredients: disodium EDTA, allantoin, panthenol, bis-PEG-18 methyl ether dimethyl silane, a thickener, sodium hyaluronate, sodium polyglutamate, a polyol, acetyl chitin, hydroxyethylpiperazine ethane sulfonic acid, alpha-arbutin, beta-dextran, glycerin polyether-26, a PEG / PPG-17 / 6 copolymer, carboxymethyl chitosan, biosaccharide gum-1, hydroxylated lecithin, hydroxyethyl urea, a nano whitening and spot-removing agent, a nano coenzyme Q10 emulsion, an anti-sensitive soothing agent, an antiseptic and antibacterial agent, and purified water. The whitening and spot-removing facial mask containing coenzyme Q10 has excellent stability and skin compatibility, has obvious whitening and spot-removing effects, is mild to the skin, and has broad application prospects.

The invention discloses a whitening and glowing skin care composition which is characterized by comprising the following components in parts by weight: 0.01-1 part of hydroxyethylpiperazine ethane sulfonic acid, 0.1-5 parts of a radix scutellariae extract, 0.1-5 parts of a peach fruit extract, and 0.1-5 parts of euglena gracilis polysaccharide. All the components act on all the ways of skin blackening respectively and cooperate with each other, so the composition can effectively inhibit tyrosinase activity, highly efficiently scavenge oxygen free radicals, prevent the production of melanin, decompose and desalinate formed melanin, significantly improve skin whiteness and skin uniformity, and eliminate the problems of dark, spotted and rough skin. At the same time, the composition also hasthe effects of moisturizing, moistening and relieving the skin, can make the skin gradually restored to a white, tender and even state of healthy skin color, and has good whitening and glowing effects.

The invention discloses a preparation method of HEPES (hydroxyethylpiperazine ethane sulfonic acid) molecule guided porous zinc oxide microspheres. The preparation method comprises the specific experimental steps of taking HEPES molecules as a guide agent and organic zinc salt as a zinc source, reacting under a solvent heat condition at 150 DEG C, performing centrifugal washing to remove the residual solvent and HEPES modules, and drying the product to obtain porous zinc oxide microspheres with high specific areas. According to the preparation method disclosed by the invention, nontoxic HEPES molecules are used, the synthetic temperature is low, the operation is simple, and the repeatability is good. The prepared porous zinc oxide microspheres have the advantages of uniform size, multistage pore structure, large specific area and the like, and can be used as a photo-catalyst and a gas-sensitive element.

The invention relates to a serum-free culture medium for amplification in vitro of a human immune killer cell, wherein the serum-free culture medium is formed by combining and mixing a basal culture medium and a serum replacement, and the serum-free culture medium comprises the basic components of 2 saccharides, 22 amino acids, 6 lipids, 2 nucleosides, 11 vitamins, 16 salts, 1 hormone, 2 protides, hydroxyethylpiperazine ethane sulfonic acid and phenol red. The serum replacement is combined and added into the basal culture medium to prepare the serum-free culture medium. The components of the serum-free culture medium are definite, and the serum-free culture medium supports amplification in vitro of the immune killer cell, achieves high efficiency and stability, promotes the further study and application of biological therapy, promotes the more smooth progressing of the immune cell therapy, and benefits the nation and the people.

The invention belongs to the technical field of organic matter preparation, and particularly relates to a method for preparing high-purity 4-hydroxyethyl piperazine ethane sulfonic acid. The method comprises the following steps: carrying out addition reaction on vinylsulfonic acid or vinyl sulfonate and N-hydroxyethyl piperazine to prepare 4-hydroxyethylpiperazine ethane sulfonate; converting the 4-hydroxyethylpiperazine ethane sulfonate into 4-hydroxyethylpiperazine ethane sulfonic acid and corresponding salts of an acidifier by adopting the acidifier, thus obtaining acidifying mother liquor; then crystallizing to remove the corresponding salts of the acidifier to obtain processed acidifying mother liquor; adding soluble barium salt or calcium salt to the processed acidifying mother liquor to remove residual sulfate radicals, and carrying out evaporative concentration to obtain a primary purified product of the 4-hydroxyethylpiperazine ethane sulfonic acid; finally, washing the primary purified product by adopting small molecular weight alcohol and drying to obtain the high-purity 4-hydroxyethylpiperazine ethane sulfonic acid. The 4-hydroxyethylpiperazine ethane sulfonic acid prepared by the method disclosed by the invention is high in purity, simple and convenient in operation process, environment-friendly in production process, low in purification cost, high in yield and suitable for industrial batch production.

The invention relates to a skin whitening and brightening composition. The skin whitening and brightening composition is made of 2%-5% of fructus evodiae extract, 1%-4% of niacinamide, 1%-4% of mulberry bark extract, 0.2%-2% of alpha-arbutin, 0.2%-2% of hydroxyethylpiperazine ethane sulfonic acid, 0.1%-1% of radix glycyrrhizae extract, 2%-6% of butanediol, 1%-5% of glycerin, 0.1%-1% of preservatives, 0-0.1% of essence and the balance water by means of mixing. The skin whitening and brightening composition has the advantages that raw materials for the skin whitening and brightening compositionare mild, the skin whitening and brightening composition is good in stability, active components of the skin whitening and brightening composition mostly come from natural plants or mostly are biological preparations, accordingly, the skin whitening and brightening composition is mild for skin, and irritation and potential risks of formulas can be reduced; synergistic effects can be realized by various components of the skin whitening and brightening composition, accordingly, integral effects can be greatly improved, and effects of reducing melanin, whitening the skin and brightening skin colors can be realized.

The invention discloses diluent of carboxylated magnetic particles and a preparation method of the diluent. The diluent is prepared from, by weight, 39-55 parts of N-(2- ethoxyl)piperazine-N'-2-ethanesulfonic acid, 28-38 parts of dipotassium phosphate, 9-18 parts of ethylene diamine tetraacetic acid, 11-29 parts of zinc chloride, 7-12 parts of magnesium chloride, 6-13 parts of heparin sodium, 15-22 parts of saccharose, 0.1-0.3 part of hydrochloric acid, 4-12 parts of glucose, 1-3 parts of bovine serum albumin, 0.2-0.6 part of mouse serums, 0.3-0.7 part of glycerin, 0.2-0.4 part of Proclin 300,0.4-0.8 part of Tween-20 and 100-300 parts of deionized water. According to the diluent of the carboxylated magnetic particles and the preparation method of the diluent, through synergistic effect ofall the components, the diluent of the carboxylated magnetic particles is prepared and used for diluting carboxylated magnetic particle mother liquor into a working solution, the working solution canbe stored for a long time, and the specificity, stability, repeatability and other performance of a reagent are not influenced.

The invention discloses a lotion with a full-stage acne removal effect and a preparation method thereof. The lotion comprises, by weight, 5-15% of full-stage acne removal compound, wherein the compound comprises water, rehmannia chinensis root extract, hydroxyethyl piperazine ethanesulfonic acid, phellodendron amurense bark extract, butanediol and centella asiatica leaf extract; 95-85% of cosmeticadjuvant matrix. The lotion is characterized in that the inhibition is performed is all stages of acne formation specific to the acne formation mechanism to achieve the safe effective acne removal effect finally.

The invention discloses a whitening and anti-freckle composition. The composition is prepared from, by weight, 2-10 parts of ascorbic acid, 0.4-2 parts of kojic acid, 0.1-2 parts of tranexamic acid, 0.1-2 parts of alpha-arbutin, 0.1-1 part of hydroxyethylpiperazine ethane sulfonic acid and a 3-15 parts of tartaric acid and / or a precursor. The whitening and anti-freckle composition reduces generation of melanin by inhibiting the activity of tyrosinase, promotes skin cell metabolism through acidized and softened cutin of the tartaric acid and / or the precursor and reducing damage of ultraviolet rays to the skin while improving the effective components by taking the hydroxyethylpiperazine ethane sulfonic acid as a biological buffer; by means of the whitening and anti-freckle composition, formation of the melanin is effectively reduced through effects in multiple aspects, and the skin is transparent. The invention further provides a skin moisturizer containing the whitening and anti-frecklecomposition and a preparation method of the skin moisturizer.

The invention discloses a moisturizing and anti-aging cosmetic formula and a preparation method thereof. The cosmetic is in a double-dosage form and is divided into an agent A and an agent B. The agent A is prepared from deionized water, butanediol, p-hydroxyacetophenone, sodium hyaluronate, hydroxyethyl piperazineethanesulfonic acid, oligopeptide-1, nonapeptide-1, glycerin, 1, 2-hexanediol and ethylhexylglycerin; the agent B is prepared from deionized water, p-hydroxyacetophenone, copper gluconate, hydroxyethyl piperazine ethane sulfonic acid and 1, 2-hexanediol. The preparation method comprises the following steps: respectively preparing the agent A and the agent B, respectively filling different bottle bodies with the prepared agent A and the prepared agent B, and packaging the agent Aand the agent B in a packaging box. The cosmetic has the beneficial effects that the cosmetic is low in treatment cost and small in risk, and each consumer can bear the cosmetic; oligopeptide-1 is selected to be combined with copper ions, and glycine-histidine-lysine-copper and hydroxyethyl piperazine ethane sulfonic acid are combined, so that the problems that the absorption rate and the effect are not obvious are effectively solved; the preparation process is simple and high in efficiency.

The invention relates to a skin repairing composition as well as a preparation method thereof and application in cosmetics. The skin repairing composition comprises the following raw materials in parts by weight: 1-10 parts of ceramide 3, 5-15 parts of hydrogenated lecithin, 5-15 parts of a hydrogenated olive oil unsaponifiable matter, 5-15 parts of a strigose oat extract, 10-20 parts of tetrahydromethyl pyrimidine carboxylic acid, 5-15 parts of a centella asiatica extract and 0.5-2 parts of ethoxy piperazine ethanesulfonic acid. By adopting the skin repairing composition provided by the invention, components take a synergetic effect into play, the self repairing capability of cells and the skin immunity can be improved inside, and the epidermal thickness can be increased and skin barriers can be repaired outside, that is, the skin repairing composition takes a skin repairing effect into play from both inside and outside, so that a remarkable skin repairing effect can be achieved, and the skin repairing composition is safe and non-irritant, and can be easily used in formula systems of various dosage forms of cosmetics.

The invention discloses a tightening and wrinkle-removing essence. The formula mainly comprises the following components in percentages by weight: 0.5-5% of a henopodium quinoa seed extract, 0.1-1% ofdipeptide diaminobutyl benzylamide diacetate and 0.1-2% of hydroxyethylpiperazine ethane sulfonic acid. The various components are matched according to a specific ratio, the various abundant functional components are mutually blended and cooperate to achieve the maximum effect, the tightening and wrinkle-removing essence effectively permeates into the dermis of skin, cell renewing and collagen synthesis are promoted, wrinkles are removed, the elasticity of the skin is improved remarkably, and an effect of tightening the skin is achieved.

The invention discloses a method for preparing an alpha type iron trioxide micro / nano material regulated by iron salt. The method comprises the following steps: adding aqueous solutions of different types of iron salts into an aqueous solution of 4-hydroxyethylpiperazine ethane sulfonic acid (HEPES), performing operations of cooling, centrifuging, washing and heat treatment according to a hydrothermal method, respectively obtaining spherical, porous rod-shaped and cuberille alpha type iron trioxide micro / nano materials with the size of 10nm to 1.8 Mum. The adopted method has the advantages of simplicity, practicability, environment friendliness, controllable product shape and high repeatability.

The invention discloses a repairing anti-aging mask containing coenzyme Q10 and a preparation method thereof. The repair anti-aging mask specifically includes: A-phase raw materials comprising 0.02-0.1% of EDTA-Na2, 0.1-0.3% of p-hydroxyacetophenone, 0.1-0.3% of allantoin, 0.2-0.5% of jojoba wax PEG-120 ester, and 1-3% of glycerin; B-phase raw materials comprising 6-8% of methylpropanediol, 0.02-0.2% of sodium hyaluronate, 0.08-0.2% of carbomer, and 0.05-0.15% of xanthan gum; a C-phase raw material comprising0.08 to 0.22% of arginine; D-phase raw materials comprising 0.1 to 0.5% of euglena gracilis polysaccharide / hydrolyzed collagen, 0.2 to 2% of beta-glucan, 0.1-0.5% of hydroxyethylpiperazine ethane sulfonic acid, 2.01-6.05% of a plant extract, 0.5-2% of a PEG / PPG-17 / 6 copolymer, 0.5-3% of bio-saccharide gum-1, and 0.3-0.8% of lipids. The anti-aging mask provided by the present invention can deeply replenish water, removes facial skin wrinkles and upper and lower eye bags, makes the skin firm, lightens dark spots, improves the skin's antioxidant ability, cannot cause skin allergies and other problems, and achieves repairing and anti-aging effects.

The invention relates to the field of a serum fibronectin detection technology, in particular to a fibronectin detection reagent. A reagent R 1 contains buffer solution, gamma-Fe2O3 / polythiophene (gamma-Fe2O3 / PTP) composite nanoparticles, fluorocarbon surfactant FC-4430 and preservative; a reagent R 2 contains buffer solution, goat anti human IgG fibronectin antibody, gelatin, xanthan glue, sodium chloride, mycose, PEG-6000, fluorocarbon surfactant FC-4430 and preservative. According to the fibronectin detection reagent, the reacting system is optimized, the stability of the reagent can be improved significantly through the adoption of HEPES (4-hydroxyethylpiperazine ethane sulfonic acid) buffer solution and the addition of a variety of stabilizers, such as sodium chloride, mycose, PEG-6000, gelatin and xanthan glue; the addition of gamma-Fe2O3 / polythiophene (gamma-Fe2O3 / PTP) composite nanoparticles in the reagent can adsorb the antigen-antibody complex through electrostatic adsorption during the detection, thus the sensitivity and accuracy degree of the reagent can be enhanced significantly. Moreover, the optimized novel fluorocarbon surfactant FC-4430 can promote and maintain the stability of the antibody, prevent the system from being muddied, and further enhance the stability of the reagent.

The invention provides a penetration enhancer for cosmetics. The penetration enhancer comprises 60-80wt% of a natural Chinese herbal medicine extract component and 20-40wt% of sodium hydroxyethylpiperazine ethane sulfonic acid sodium, the natural Chinese herbal medicine extract component comprises at least one of tetrahydropiperine, cinnamaldehyde, eugenol and tanshinone, and beta-1,3-glucan is grafted with the natural Chinese herbal medicine extract component to carry out modification treatment. The natural Chinese herbal medicine extract component in the penetration enhancer for cosmetics has good endophilicity to skins, is safe and non-irritant, increases the penetration promotion effect through glucan modification, also can control just action of the penetration promotion effect on stratum corneum, and improves the use safety of the cosmetics. The penetration enhancer for cosmetics has the advantages of short onset time, realization of the penetration promotion time lag being shorter than 10min, small dosage, and high penetration promotion rate, and the penetration promotion rate of the penetration enhancer provided by the invention is 4 or more times that of traditional azone penetration enhancers when the dosage of the penetration enhancer provided by the invention is 1 / 20 of the dosage of the traditional azone penetration enhancers.

The invention relates to a composition for whitening and brightening skin and an application of the composition for whitening and brightening skin to cosmetics. The composition comprises the followingcomponents of a glabridin carrier, hydroxyethylpiperazine ethane sulfonic acid (HEPES), an endothelin antagonist and plant composite essence. The composition for whitening and brightening skin can effectively restrain tyrosinase activity, restrain melanocyte disintegration, eliminate free radicals, soften cutin, improve skin withstanding capacity and the like; a glabridin carrier technique and plant fresh and living ingredients coexist, powerful efficacy of whitening skin and lightening freckles of the endothelin antagonist is used for cooperation, in the presence of the HEPES, synergistic interaction is achieved, and excellent efficacy of whitening skin and brightening skin can be achieved.

The invention discloses a pig oocyte in-vitro maturation culture solution and a preparation method and application thereof, and belongs to the technical field of oocyte in-vitro maturation culture. The problems of low in-vitro maturation rate and development rate of porcine oocytes currently are solved. The pig oocyte in-vitro maturation culture solution is prepared from a basal culture solution TCM-199, penicillin, streptomycin, NaHCO3, 4-hydroxyethylpiperazine ethane sulfonic acid, polyvinyl alcohol, sodium pyruvate, insulin, cysteine, epidermal growth factor, porcine follicular fluid, pregnant mare serum gonadotropin, human chorionic gonadotropin and tannic acid. According to the pig oocyte in vitro maturation culture solution, the oocyte in-vitro maturation quality, the cumulus cell diffusion index, the parthenogenetic embryo blastocyst rate and blastocyst total cell number, and the in-vitro fertilization embryo cleavage rate, blastocyst rate and blastocyst total cell number can beimproved.

The invention relates to a tender and smooth hand cream composition, which is prepared from the following components in parts by weight: 25 to 35 parts of rice fermentation liquid and yeast extract, 2to 5 parts of hydroxyethyl piperazine ethane sulfonic acid, 3 to 8 parts of nicotinamide and 8 to 15 parts of water. The invention also provides a hand cream containing the tender and smooth hand cream composition, and preparation methods of the tender and smooth hand cream composition and the hand cream. The tender and smooth hand cream composition disclosed by the invention not only can play arole in moisturizing, and remarkably repair hand skin, but also can realizes exfoliating and permeation-assisting effects, so that the hand skin is tender and smooth and brightened. The composition disclosed by the invention has the advantages of repairing, moisturizing, nature, mildness, safety and high skin tenderness, and the preparation method is simple.

The invention relates to a whitening and freckle-removing composition, whitening and freckle-removing cream and production methods of the whitening and freckle-removing composition and the whitening and freckle-removing cream. The whitening and freckle-removing composition comprises the raw material components: 3-o-ethyl-l-ascorbic acid, alpha-arbutin, corn starch, hydrolyzed corn starch, hydrolyzed corn starch octenylsuccinate, a shea butter extract, amides, coco, n-[3-(dimethylamino)propyl], 4-(alpha-methylbenzyl)resorcinol, 2-mercaptopropionic acid, palmitoyl tripeptide-5, hydrated silica and hydroxyethylpiperazine ethane sulfonic acid. By using the whitening and freckle-removing composition in a skin care product, reduction, caused by ultraviolet light, of skin brightness can be significantly inhibited, meanwhile, decomposition and excretion of melanin can be accelerated, and skin pigmentation is reduced, so that the skin care product has double effects of whitening and freckle removing, the raw materials are natural, and the use safety for a human body is high.

The invention relates to animal vaccines, and specifically discloses an antigen of an epidemic encephalitis live vaccine and a preparation method and application thereof. Vero cells are adopted to carry out roller bottle culture on epidemic encephalitis virus attenuated strains (SA14-14-2 strains); virus culture maintenance solution which comprises components such as 4-hydroxyethylpiperazine ethane sulfonic acid, arginine monohydrochloride and PEG2000 is used; the produced antigen has the advantages of being small in batch difference, good in immunogenicity and high in virus titer; and the epidemic encephalitis live vaccine prepared through the antigen is capable of effectively preventing the epidemic encephalitis virus infection of pigs.

The invention discloses a light-sensitive whitening essence and a preparation method thereof. The light-sensitive whitening essence is prepared from a humectant, a chelating agent, bis-PEG-15 methyl ether polydimethylsiloxane, carnosine, PEG-60 hydrogenated castor oil, hydroxyethyl piperazine ethane sulfonic acid, arginine, vitamins, a composite plant extracting solution, a herba portulacae extract, a hydrolyzed Prunus domestica extract, a preservative, a whitening additive and the balance of water. According to the essence disclosed by the invention, the pH value of a semi-finished product ofthe essence is maintained between 6.5 and 8.2 through the arginine and the hydroxyethyl piperazine ethane sulfonic acid; when the whitening essence is used, the pH value is 4.0-5.5 after the whitening additive and the essence semi-finished product are mixed; the stability and activity of phenethyl resorcinol and ascorbyl glucoside are guaranteed, and through the synergistic effect of the hydrolyzed Prunus domestica extract, the carnosine and the like, generation of melanin can be effectively reduced, color spots are faded, the skin color is homogenized, and the skin is whitened and brightenedfrom inside to outside.

The invention discloses a composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The composition comprises the following components in parts by weight: 0.01-0.18 part of hydrolyzed sclerotium gum, 0.1-1.5 parts of hydroxyethyl piperazine ethanesulfonic acid, 0.15-1.5 parts of acetylchitosamine, 0.3-2 parts of niacinamide, 0.3-3 parts of rose water, 0.001-0.009 part of dimethyl silanol hyaluronate, 0.001-0.009 part of phenoxyethanol, 0.0005-0.0045 part of ethylhexylglycerin, 0.13-0.675 part of glycerin, 0.02-0.075 part of diglucosyl gallic acid and 0.5-5.0 parts of saccharomycetes polypeptide. The composition provided by the invention has multiple effects of moisturizing, resisting wrinkles, whitening, relieving, repairing and the like.

The invention discloses a limbus corneae stem cell serum-free medium and a culture method thereof. The serum-free medium comprises a basic culture medium and additives, wherein the additives include human recombinant EGF, insulin, 3,3',5-triiodo-L-thyronine, hydrocortisone, forskolin, manganese sulfate monohydrate, sodium selenite, sodium metasilicate, ammonium metavanadate, nickel chloride hexahydrate, stannous chloride dihydrate, ethanol amine, phosphorylethanolamine, ammonium molybdate tetrahydrate, 4-hydroxyethylpiperazine ethane sulfonic acid, vitamin C, bovine serum albumin, lipid concentrate and serum substitution. The serum-free medium contains no fetal calf serum or any animal source ingredient, can supply sufficient nutrients and good environment needed by cell growth proliferation and can effectively replace the effect of serum, cells grow well, the cell purity and stability are improved, rapid and stable cell sources are provided for limbus corneae stem cell specificity mechanism research and transplantation therapy, and the clinic application prospect is wide.

The invention discloses a whitening 5D compound with a synergistic whitening effect. The whitening 5D compound is prepared from the following components in parts by weight: 0.5-2 parts of nicotinamide; 0.15-0.40 part of phenylethyl resorcinol; 0.35-0.8 part of undecylenoyl phenylalanine; 4.5-10 parts of ascorbic acid; and 0.45-1 part of hydroxyethyl piperazine ethanesulfonic acid. The invention further provides a whitening cosmetic prepared from the whitening 5D compound with the synergistic whitening effect and a corresponding preparation method of the whitening cosmetic. The whitening effect of the product is enhanced through the combined action of multiple mechanisms, and meanwhile, no obvious irritation is caused to the skin.

The invention provides a Chinese eaglewood skin-moisturizing lotion and a preparation method thereof. The lotion is prepared from the following raw materials: 8-12% of butanediol, 4-6% of propylene glycol, 4-6% of squalane, 1.8-2.2% of nicotinamide, 0.8-1.2% of lactobacillus / eriodictyon californicum ferment extract, 1.3-1.8% of at least one of polyacrylamide, C13-14 isoparaffin and laureth-7, 0.8-1.2% of ceramide 1, 0.8-1.2% of stephania tetrandra extract, 0.4-0.6% of Chinese eaglewood extract, 0.8-1.2% of at least one of hydrocotyle asiatica root extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea extract, glycyrrhiza glabra root extract, chamomilla recutita flower extract and rosmarinus officinalis leaf extract, 1.8-2.2% of at least one of taurine, glutathione, tranexamic acid, potassium methoxysalicylate, hydroxyethylpiperazine ethane sulfonic acid, and azelamide MEA, 0.7-0.9% of yeast extract, 0.04-0.06% of leuconostoc / radish root ferment filtrate,0.08-0.12% of allantoin, 0.08-0.12% of xanthan gum, 0.08-0.12% of carbomer, 0.08-1.2% of triethanolamine, 0.25-0.35% of p-hydroxyacetophenone, 0.8-1.2% of 1,2-hexanediol, 0.1-0.2% of sodium hyaluronate and the balance of water. The prepared skin-moisturizing lotion has good moisturizing, whitening, antibacterial and skin-moisturizing effects.

The invention provides a method for preparing a novel competent cell (C-competent cell for short) and a transformation step thereof. The method comprises the following preparation processes: cultivating escherichia coli, centrifugally collecting bacteria, collecting the bacteria after re-suspending the bacteria by a buffer solution 1, collecting the bacteria after re-suspending the bacteria by a buffer solution 2; and split charging for later use. In the preparation method, the buffer solution 1 is a water solution containing the following components: magnesium chloride (10mM-1M), potassium chloride (10mM-1M), calcium chloride (10mM-1M), glucose (0.1-5%), and 4-hydroxyethylpiperazine ethane sulfonic acid (10mM-100mM), the pH value is 6.5; the buffer solution 2 is a water solution containing the following components: calcium chloride (10mM-1M), sodium chloride (10mM-1M), ferric chloride (10mM-1M), and dimethylsulfoxide (DMSO) (5-30%), and the pH value is 7.0. The transformation process comprises the following steps: thawing the C-competent cell on ice; adding to-be-transformed plasmid DNA when thawing for 1 / 3 hours; and evenly mixing and transforming into a cloning coated plate to cultivate.

The invention discloses a porcine oocyte in-vitro maturation culture solution as well as a preparation method and application thereof and belongs to the technical field of oocyte in-vitro maturation culture. In order to solve the problems of low porcine oocyte in-vitro maturation rate and development rate at present, the invention provides the porcine oocyte in-vitro maturation culture solution aswell as the preparation method and application thereof. The culture solution comprises a base culture solution TCM-199, penicillin, streptomycin, NaHCO3, 4-hydroxyethylpiperazine ethane sulfonic acid, polyvinyl alcohol, sodium pyruvate, insulin, cysteine, an epidermal growth factor, a porcine follicular fluid, pregnant mare serum gonadotropin, human chorionic gonadotropin and ramelteon. The culture solution can increase the oocyte in-vitro maturation quality, the cumulus cell diffusion index, the glutathione level, the parthenogenetic embryo blastocyst rate and blastocyst total cell number, the in-vitro fertilization embryo cleavage rate, the blastocyst rate and blastocyst total cell number and decrease the ROS level of oocytes.

The invention belongs to a fluorescent chemical sensing technology, and provides a fluorescent probe for zinc ion and cadmium ion detection as well as a preparation method and application of the fluorescent probe. The probe has a recognition function on zinc ions and cadmium ions; the probe, zinc ions and cadmium ions take a reaction in acetonitrile-HEPES (hydroxyethylpiperazine ethane sulfonic acid) buffer liquid (pH=7.4, v / v=1:1), so that fluorescence of the probe is quenched; the lowest detection concentration on the zinc ions is 0.39 mumol / L; the lowest detection concentration on the cadmium ions is 0.34 mumol / L. Therefore test paper containing the probe can be used for naked-eye qualitative verification on the zinc ions and the cadmium ions.