2 results about "Hydrocotyle" patented technology

This invention belongs to the technical field of separation and extraction of bioactive components, specifically relating to a method for separating intracellular water-soluble proteins from *Hydrocotyle erythrorhizon*, comprising the following steps: Step 1, preparation of extraction medium: preparing a copolymer solution from an amphiphilic temperature-responsive block copolymer; Step 2, mixing and permeation: mixing *Hydrocotyle erythrorhizon* algal powder with the copolymer solution prepared in Step 1 to form a mixed slurry, and stirring for 30 to 180 minutes; Step 3, thermally triggered separation: heating to 32°C to 45°C, maintaining the temperature and allowing it to stand for 10 to 60 minutes to allow the system to separate into phases, and collecting the condensed phase rich in water-soluble proteins; Step 4, protein recovery: dialysis and drying the condensed phase collected in Step 3 to obtain the protein product. This invention, by employing a temperature-responsive block copolymer, integrates the gentle disruption of cell walls, selective capture, and thermally triggered preliminary separation of intracellular water-soluble proteins from *Hydrocotyle erythrorhizon* into a continuous aqueous process, which is beneficial for maintaining protein activity.

The invention discloses a production process for crude extraction of 95% ecdysone from cyanotis arachnoidea, and particularly relates to the technical field of plant separation and extraction. The preparation process comprises the steps of raw material preparation, macroporous resin adsorption and crystallization purification. According to the method disclosed by the invention, high-purity extraction of the ecdysone and remarkable shortening of the production period are realized by adopting the steps of crushing and extracting the fresh cyanotis arachnoidea, adjusting the pH value by lime milk, adsorbing and desorbing by macroporous resin and the like. Compared with a traditional production process, the method has the advantages that the production cost is lower, the production efficiency is higher, and reliable technical support is provided for large-scale production of ecdysone. The invention also relates to an application of 55%-60% HPLC of the cyanotis arachnoidea extract. Through a solvent crystallization method, high-purity ecdysone can be further extracted from the Cyanotis arachnoidea extract. The method has the characteristics of high efficiency and practicability, and can provide a more stable raw material source for production of ecdysone.