132 results about "Phytophthora" patented technology

The invention belongs to the field of biological prevention and control of plant diseases, and particularly discloses a biocontrol bacterium bacillus velezensis and application thereof in preparation of a biocontrol inoculant for preventing and controlling pepper phytophthora blight, the strain is bacillus velezensis Y426-7, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 23, 2024, and has the preservation number of CGMCC NO.32041, and the preservation number is CGMCC NO.32041. The biocontrol bacterium is bacillus velezensis Y426-7. The bacillus velezensis is preserved in No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and can be used for preparing a biocontrol inoculant for preventing and treating pepper phytophthora blight. Compared with the prior art, the bacillus velezensis Y426-7 obtained by the invention has an obvious antibacterial effect on phytophthora capsici and various crop phytophthora blight bacteria under an indoor in-vitro condition; furthermore, the 2.5 * 10 < 9 > CFU / g wettable powder prepared from the bacillus velezensis Y426-7 has a relatively good prevention and treatment effect and a certain yield increasing effect on the pepper phytophthora blight in the field.

The invention discloses a preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight. The novel preparation method of the microalgae nano-selenium comprises the following steps: firstly, extracting an intracellular extract of chlorella pyrenoidosa, and then adding sodium selenite into the intracellular extract to carry out nano-selenium conversion; compared with an existing reported method that after sodium selenite is added into a microalgae living body culture solution to be converted into nano-selenium, algae is taken to break cells, and the nano-selenium is collected, the method has the advantages that the microalgae living body does not need to be used when the nano-selenium is biologically converted, and the subsequent complicated nano-selenium separation and purification steps can be avoided; the nano-selenium obtained by converting the intracellular extract can be directly applied. Experiments prove that the effect of the nano-selenium obtained through biotransformation on inhibiting phytophthora infestans of potato late blight pathogenic bacteria is obviously better than that of chemically synthesized nano-selenium, and the nano-selenium obtained through biotransformation has an obvious effect on preventing and treating potato late blight and has an obvious effect on promoting plant growth.

The invention belongs to the technical field of modern agriculture, and particularly relates to a biocontrol bacillus amyloliquefaciens BA200 and application thereof. A new biological control bacterium BA200 is separated and identified, is named as bacillus amyloliquefaciens by taxonomy, and has a remarkable inhibition capability on two pathogenic bacteria, namely fusarium oxysporum and phytophthora vigna, causing the cowpea fusarium wilt, and the inhibition rate is up to 60% or above; fermentation liquor prepared from the bacillus amyloliquefaciens BA200 can remarkably reduce the morbidity and disease index of cowpea fusarium wilt, is a good biocontrol microorganism and has application and development potential and important significance in control of cowpea fusarium wilt, and the biocontrol bacterium bacillus amyloliquefaciens BA200 has a broad-spectrum biological control effect, can inhibit various phytopathogens and has a good application prospect. And valuable biocontrol bacterium resources are provided for preparation of biological bactericides.

The invention relates to the technical field of biological control, and discloses bacillus siamensis and application thereof. The bacillus siamensis disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.32576. The bacillus siamensis has a good control effect on pepper phytophthora blight, the field control effect can reach 71.69 + / -8.00%, meanwhile, the bacillus siamensis can antagonize various plant pathogenic fungi in a broad spectrum, and the bacillus siamensis can be used for preventing and treating various plant diseases.

Bacilli and combinations of Bacilli capable of germinating and growing under low temperature conditions; of associating with plant roots; of enhancing plant health, growth, and yield; and of protecting plants against phytopathogenic pests, such as Botrytis, Fusarium, Phytophthora and Pythium.

The invention discloses an application of a small peptide PEP914 or PEP890 in preparation of a biopesticide, and belongs to the technical field of biology. The biopesticide comprises a small peptide PEP914 or PEP890 and is a biopesticide for resisting bacterial leaf spot, rust disease or phytophthora root rot, the amino acid sequence of the small peptide PEP914 is shown as SEQ ID No.1, and the amino acid sequence of the small peptide PEP890 is shown as SEQ ID No.2. The small peptide PEP914 or PEP890 is applied to preparation of the biopesticide, a novel biopesticide thought is provided, and the problems that in the prior art, chemical pesticides are prone to remaining, environment problems are prone to being caused, and pathogens generate drug resistance are solved.

The invention relates to application of mitogen-activated protein kinase PiPmk1 in screening molecules for inhibiting formation of a phytophthora infestans infection structure. The invention finds that the molecular marker plays a key role in the development of a phytophthora infestans cyst bud tube and / or the formation of an infection structure, which indicates that the molecular marker has the potential of serving as a target for inhibiting phytophthora infestans infection, and a corresponding molecular screening method and a generative prediction model are further developed and utilized; rapid and accurate prediction and screening of phytophthora infestans infection inhibitory molecules are realized, the research and development period and cost are greatly shortened, and a new thought and a new method are provided for development of accurate targeted green pesticides.

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention discloses an ecological cultivation method for preventing pepper phytophthora blight, which comprises the following steps: step 1, soaking pepper seeds in warm water at 40-50 DEG C for 10-30 minutes, soaking for a short time to achieve the effect of softening seed coats, soaking the pepper seeds in normal-temperature water at 20-25 DEG C for 4-8 hours after soaking in warm water to ensure that the seeds absorb sufficient water, and then soaking the pepper seeds in the warm water for 10-30 minutes; soaking the chilli seeds after absorbing water in a disinfectant, thoroughly washing the seeds with clear water for 2-3 times after soaking and disinfection, and taking out the residual disinfectant; according to the ecological cultivation method for preventing pepper phytophthora blight, the disease resistance is remarkably improved through seed multi-stage treatment (gradient water temperature soaking, composite disinfection and three-bacterium coating), and the pathogen infection risk is reduced by combining seedling culture substrate bacteriostasis and a degradable non-woven fabric root protection technology; the comprehensive income is improved through application of the degradable material and basil intercropping, and finally the occurrence rate of pepper phytophthora blight is reduced.

The invention discloses application of exogenous selenium to improvement of tomato late blight resistance, and belongs to the field of plant disease control. The exogenous selenium comprises Na2SeO3 (sodium selenite). Compared with a control group, exogenous selenium can inhibit the morbidity of tomato plants. Specifically, the exogenous selenium can inhibit the expansion of tomato leaf late blight scabs, relieve the accumulation of H2O2 and O2 <-> in the leaves and reduce the ROS content in the tomato leaves; meanwhile, the activity of antioxidant enzymes SOD, POD and CAT and the content of chlorophyll can be improved, and generation of MDA is reduced; besides, the content of SA and JA of tomato plants and the content of selenium in tomato leaves and fruits can also be increased by spraying exogenous selenium; in addition, through detection, exogenous selenium can improve the resistance of tomatoes to phytophthora infestans by inducing expression of the SIPR5 gene. In conclusion, the invention finds that the exogenous selenium can improve the resistance of the tomato to the late blight, and provides a new method for preventing and treating the tomato late blight.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of RIN4 family genes in improving resistance of tomatoes to phytophthora infestans. Two genes related to phytophthora infestans infection are found and verified in tomatoes, the genome nucleotide sequences of the two genes are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes belong to RIN4 gene family members. The gene is knocked out or silenced through a gene editing technology, and it is found that the tomato strain with the gene knocked out or silenced has higher phytophthora infestans resistance compared with a wild type. Meanwhile, tomato strains edited by the genes are not obviously influenced in the aspects of growth and development. Therefore, the invention not only enriches the research of phytophthora infestans susceptibility related genes in tomatoes, but also develops a tomato strain with enhanced phytophthora infestans resistance by utilizing the characteristics of the genes, and provides a new technical strategy and gene resources for tomato breeding.

The invention discloses application of alfalfa VIK protein in cultivation of phytophthora root rot resistant plants, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to improve the disease resistance of plants. The VIK protein disclosed by the invention is a protein of which the amino acid sequence is SEQ ID No. 1 or SEQ ID No. 4. Experiments prove that after the phytophthora phytopathogen is inoculated, compared with a wild plant, the mutant obtained by editing the VIK coding gene has the advantages that the disease resistance is enhanced, the survival rate is improved, the fresh weight, the overground part height, the root length and the plant height of the plant are increased, and the relative conductivity of leaves is reduced. Therefore, the resistance of the plant to the phytophthora phytopathogen is improved through the mutation of the VIK protein and the coding gene thereof, the coding gene of the VIK can be targeted through a gene editing technology, a VIK gene knockout plant is obtained, and a plant resisting the phytophthora phytopathogen is cultivated.

The invention discloses a preparation method of a biochar-based bacterial fertilizer capable of relieving pepper phytophthora blight. The biochar-based bacterial fertilizer takes nutrient substances as an inner shell and beneficial microorganisms as an outer shell; the inner shell is prepared from the following components in percentage by mass: 21 to 32 percent of urea, 18 to 20 percent of monopotassium phosphate, 6 to 15 percent of potassium sulfate, 3 to 5 percent of humic acid, 3 to 8 percent of oxidized starch, 0 to 10 percent of kaolin, 0 to 10 percent of diatomite and 0 to 20 percent of charcoal; the shell is prepared from the following components in percentage by mass: 10 to 20 percent of biochar for adsorbing beneficial microorganisms and 3 to 8 percent of starch; the inner shell material and the outer shell material of the biochar-based bacterial fertilizer are uniformly mixed, 1% aminobutyric acid is sprayed for granulation, then the mixture is put into a drying oven to be dried at 45 DEG C, and finally a polyurethane coating agent is used for coating. The biochar-based bacterial fertilizer can improve the soil environment, increase the fertilizer utilization rate and reduce the occurrence of pepper phytophthora blight.

The invention discloses an endophytic subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. The strain can inhibit the growth of different physiological races of phytophthora infestans, and has inhibitory activity on various pathogenic oomycetes. The fermentation liquor of the strain contains micromolecular antibacterial substances with relatively high stability, and has remarkable antibacterial activity; protein components in fermentation liquor can induce plants to generate resistance, and infection of phytophthora infestans is effectively inhibited. Different types of microbial agents prepared by utilizing the strain DS1 can be used for effectively preventing and treating potato late blight, and the microbial preparation is used for seed dressing treatment of potatoes and wheat and can be used for remarkably promoting the growth of crops.

The invention belongs to the technical field of microorganisms, and discloses a Trichoderma brevicompactum 6311 with phytophthora capsici biological control ability, the name of the Trichoderma brevicompactum 6311 is 6311, the classification name of the Trichoderma brevicompactum is Trichoderma brevicompactum 6311, the preservation number of the Trichoderma brevicompactum 6311 is CGMCC No.41489, the preservation date of the Trichoderma brevicompactum 6311 is August 13, 2024, and the preservation unit of the Trichoderma brevicompactum 6311 is China General Microbiological Culture Collection Center, No. 3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The fermentation liquor of the trichoderma 6311 has the capability of inhibiting the growth of phytophthora hyphae on a flat plate, and the inhibition rate reaches 100%. The germination rate, radicle and hypocotyl length, growth index and fresh weight of the pepper seeds soaked in the 6311 fermentation liquor are improved. The research shows that the trichoderma brevicompactum 6311 has biological control potential and provides a theoretical basis for application of the trichoderma brevicompactum 6311 as a biological bactericide.

This invention discloses the CAPE1 peptide at the C-terminus of the PtPR1 protein and its applications. There are three types of CAPE1 peptides in the PtPR1 protein: PtPR1f (amino acid sequence shown in SEQ ID NO.1), PtPR1j (amino acid sequence shown in SEQ ID NO.2), and PtPR1l (amino acid sequence shown in SEQ ID NO.3). Experimental results show that the three CAPE1 peptides at the C-terminus of the PtPR1 protein provided by this invention can induce an immune defense response in woody plants at low concentrations. The use of these three CAPE1 peptides can also significantly improve the disease resistance of woody plants to *Colletotrichum gloeosporioides*, *Pseudomonas aeruginosa*, *Botrytis cinerea*, or *Phytophthora spp.*, without inhibiting the growth of these pathogens. Therefore, it can be applied to the prevention and control of woody plant diseases, providing a new approach to improving the disease resistance of woody plants and showing broad application prospects in the green control of woody plant diseases.

The present invention discloses a primer set and a detection method thereof for synchronously detecting a plurality of quarantine pathogens of soybean. The primer set is composed of a detection primer pair SoqF / SoqR for Phytophthora sojae, a detection primer pair DPM1F / DPM1R for southern soybean stem canker pathogen, a detection primer pair DPC2F / DPC2R for northern soybean stem canker pathogen, and a detection primer pair FV3F / FV3R for North American soybean sudden death syndrome pathogen. The primer set is added to the same PCR reaction system, and specific amplification products of different sizes are obtained by multiple PCR amplification and agarose gel electrophoresis. The primer set and the detection method of the present invention have strong specificity and high sensitivity, and can quickly and accurately synchronously detect a plurality of quarantine pathogens in soybean in a system. The primer set can be used for port inspection and quarantine, early diagnosis of field diseases, and monitoring and identification of pathogens.

The application discloses application of a fungal virus in prevention and treatment of pear tree and apple tree Valsa diseases. The fungal virus is a positive single-stranded RNA virus, and a cDNA nucleotide sequence of the fungal virus is shown as SEQ ID No. 1. A fungus containing the virus is Valsa pyri HJL1-22 (gamma), the fungus is infected by the fungal virus of claim 1, the fungus is named as HJL1-22 (gamma), is classified and named as Valsa pyri, and has a preservation number of CGMCC No. 41449 in China General Microbiological Culture Collection Center. The virus Vphv1-gamma can be secreted to outside of cells, therefore, culture liquid filtered off mycelium can be used as a biocontrol agent for prevention and treatment of pear tree and apple tree Valsa diseases, and can effectively weaken or inhibit pathogenicity and diffusion of Valsa pyri and Valsa mali, and has a prospect for being used for producing and practicing biological prevention and treatment of pear tree and apple tree Valsa diseases.

The invention discloses a Trichoderma nordicum strain and an application of the Trichoderma nordicum strain, and particularly discloses a Trichoderma nordicum strain V1, and the preservation number of the Trichoderma nordicum strain V1 is CGMCC (China General Microbiological Culture Collection Center) No: 41478. According to the present invention, the Trichoderma nordicum strain V1 provided by the invention has strong cellulase and protease secretion ability, and the Trichoderma nordicum strain V1 has strong cellulase and protease secretion ability; afterwards, an antagonism experiment verifies that the V1 strain can effectively kill phytophthora capsici and pythium aphanidermatum. The biocontrol effect of the trichoderma peucedanum strain on pepper phytophthora blight and cucumber seedling damping-off is researched through indoor pot experiments, and the growth promoting effect of the trichoderma peucedanum strain on pepper seedlings is explored. Research results show that V1 treatment has very remarkable prevention, treatment and growth promotion effects.

The invention discloses a bis (N-acylamino-3, 1-benzoxazinyl) methane compound and a preparation method and application thereof.The compound takes an arylamine compound and bromoacetyl bromide as raw materials, triethylamine as an acid-binding agent and dichloromethane as a solvent to react in an ice-water bath to obtain an N-aryl-2-bromoacetyl arylamine compound, then the N-aryl-2-bromoacetyl arylamine compound is subjected to multi-step reaction, and the bis (N-acylamino-3, 1-benzoxazinyl) methane compound is obtained. Finally, the bis (N-acylamino-3, 1-benzoxazinyl) methane compound with bactericidal activity is obtained. The raw materials for synthesis of the compound are cheap and easy to obtain, and the synthesis method is simple. Meanwhile, the compound has a good inhibition effect on the activity of crop germs, especially has remarkable inhibition activity on sclerotinia sclerotiorum, phytophthora, gibberellic disease bacteria, magnaporthe oryzae, botrytis cinerea and rhizoctonia solani, and has remarkable application value in the aspects of crop disease control and drug resistance challenge.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

The present invention relates to bio-fungicides that inhibits Phytophthora infestans. More particularly, the present invention relates to novel strains of Bacillus spp. that can inhibit Phytophthora infestans. The present invention also relates to bio-fungicidal formulations comprising novel strains of Bacillus spp. either alone or a mixture of novel strains of Bacillus spp., in combination with other excipients.

The invention discloses a 3-ester-1, 3-quinazoline-2, 4-diketone compound and a preparation method and application thereof.The 3-ester-1, 3-quinazoline-2, 4-diketone compound is prepared by the steps that amino-acid ester hydrochloride and isatoic anhydride serve as raw materials and react in acetonitrile under the action of potassium carbonate to generate an amide ester compound; and then reacting the amide ester compound with triphosgene under the action of triethylamine to obtain the 3-ester-1, 3-quinazoline-2, 4-diketone compound. Raw materials for synthesis of the compound are cheap and easy to obtain, the synthesis method is simple, meanwhile, the compound has good bacteriostatic activity on crop germs, especially has a remarkable activity inhibition effect on germs such as gibberellic disease bacteria, phytophthora germs, pyricularia grisea, sclerotinia sclerotiorum, botrytis cinerea and rhizoctonia solani, and the yield of crops is well guaranteed.

The invention provides application of a DTX29 gene and a protein thereof in improving the resistance of plants to phytophthora infestans. The invention finds a susceptible gene DTX29 capable of effectively improving the resistance of plants to phytophthora infestans through a large number of researches. The DTX29 gene of the plant is edited through a CRISPR-Cas technology and the like, so that the function of the DTX29 gene is lost, the resistance of the plant to phytophthora infestans can be effectively improved, the diameter and area of disease spots and the relative biomass of phytophthora infestans are reduced, and normal growth and development of the plant are not influenced. The invention provides a new target and idea for prevention and control of plant diseases caused by phytophthora infestans.

The invention discloses a bacillus amyloliquefaciens BS-3 and an antibacterial protein and application derived therefrom, belonging to the technical field of microorganisms and genetic engineering. The invention discloses a bacillus amyloliquefaciens BS-3, and the deposit number is CCTCC NO: M 2024893. The antibacterial protein derived from Bacillus amyloliquefaciens BS-3, and its amino acid sequence is shown in SEQ ID NO.7. Bacillus amyloliquefaciens BS-3 can inhibit Colletotrichum oxysporum, Alternaria rubber, Pseudomonas microsporus, Phytophthora litchii, Phytophthora cucurbitae, Phytophthora capsici, and Citrus black spot pathogen. The antibacterial protein derived from Bacillus amyloliquefaciens BS-3 can inhibit Colletotrichum oxysporum, Alternaria rubber, and Pseudomonas microsporus.

The invention belongs to the technical field of biology, and particularly relates to a denitrifying hyphomicrobium high-producing strain for producing pyrroloquinoline quinone (PQQ) and application of the denitrifying hyphomicrobium high-producing strain. In particular, the present invention provides an isolated denitrifying hyphomicrobium strain comprising a 16S rDNA sequence having at least 96% identity with one or more of SEQ ID NO: 1. The invention further provides a separated denitrifying hyphomicrobium strain, and the separated denitrifying hyphomicrobium strain comprises the denitrifying hyphomicrobium strain SHYZPQQ001 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252085, namely the denitrifying hyphomicrobium strain SHYZPQQ001.

In one aspect, the present application discloses compositions of extracellular vesicles (EVs) comprising heterologous effector RNAs, such as antifungal RNAs. In certain embodiments, the effector RNAs comprise dsRNA, while in other embodiments the effector RNAs comprise mRNA In certain embodiments, the EVs are plant EVs, microbial EVs (e.g., bacterial EVs or fungal EVs), or artificial EVs. In particular embodiments, the EVs are plant EVs and the effector RNA is mRNA. In other particular embodiments, the EVs are microbial EVs and the effector RNA is dsRNA. In other aspects, the application discloses microbes that produce RNA-loaded EVs; methods of treating and / or preventing fungal diseases by applying such microbes and / or RNA-loaded EVs to plants, plant parts, and / or soil; and methods of producing such RNA-loaded EVs. In certain examples, the antifungal RNAs may target DCL1 / 2 genes the VDS genes, and / or the tetraspanin (PLS1) genes of a fungal pathogen such as B. cinerea, Verticillium dahliae, Sclerotinia sclerotiorum, Fusarium oxysporum, Fusarium graminearum, Aspergillus niger or even oomycete pathogens, such as Phytophthora infestans.

The present application provides a kind of compound biocontrol agent and its preparation method and application technology, and it is composed of 1000ml of fermentation mixed liquor of multiple strains of biocontrol bacteria mixed in equal volume ratio and 0.5%-1.5% stabilizer with complementary mechanism and target.The fermentation concentrated solution of multiple strains of bacteria is prepared in fermentation medium to obtain the present application, which is used for preventing and controlling fusarium, pythium, phytophthora, rhizoctonia solani, ralstonia solanacearum, erwinia carotovora, streptomyces scabiei, xanthomonas campestris and other plant pathogenic bacteria and nematodes, which can inhabit in soil for a long time and spread through soil, and one or several of them can cause plant soil-borne diseases.The preparation process of the present application is not affected by too many chemical and physical factors, the activity of live bacteria and metabolites is high, the average control effect is 78%-85%, the application effect is good;The production process is simple, energy and auxiliary materials are saved, and the production cost is low.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

The present invention discloses the application of alfalfa VIK protein in cultivating plants resistant to Phytophthora root rot, and belongs to the field of biotechnology. The technical problem solved by the present invention is how to improve the disease resistance of plants. The VIK protein disclosed in the present invention is a protein whose amino acid sequence is SEQ ID No.1 or SEQ ID No.4. Experiments have shown that after inoculation with Phytophthora plant pathogens, compared with wild-type plants, the mutants obtained by editing the VIK encoding gene have enhanced disease resistance and improved survival rate, increased plant fresh weight, above-ground part height, root length, and plant height, and decreased leaf relative conductivity. Therefore, mutations in the VIK protein and its encoding gene improve the plant's resistance to Phytophthora plant pathogens. The VIK encoding gene can be targeted by gene editing technology to obtain VIK gene knockout plants and cultivate plants resistant to Phytophthora plant pathogens.