293 results about "Phytophthora" patented technology

A fungicidal composition for plants containing phosphonate (PO3) and phosphate (PO4) salts, and derivatives thereof is disclosed. The composition provides a single product which may be employed to control a Phytophthora infestans infection in plants.

The invention belongs to the technical field of biological pesticides, discloses a Streptomyces l avendulae xjy strain which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the Streptomyces l avendulae xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by Streptomyces xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.

The invention relates to a method for obtaining a capsicum phytophthora resistance candidate gene and a molecular marker, and application. The method is used for obtaining the capsicum phytophthora resistance candidate gene by utilizing capsicum phytophthora transcriptome and whole-genome sequencing data information, differentially-expressed gene identification, bioinformatics analysis, molecular marker development and phytophthora inoculation identification and belongs to the technical field of capsicum biology. The method comprises the following steps: sequencing a phytophthora resistant and susceptible gene pool transcriptome obtained after phytophthora inoculation of an F2 population constructed by capsicum highly-resistant and highly-susceptible phytophthora materials, performing expression analysis and functional annotation on differential genes, extracting DNAs (Desoxvribose Nucleic Acid) of a capsicum phytophthora highly-resistant and highly-susceptible phytophthora material genome, performing primer design and PCR (Polymerase Chain Reaction) amplification, performing sequence difference analysis and SNP site identification, performing SNP specific primer design and validity verification, and performing other steps to efficiently obtain the capsicum phytophthora resistance candidate gene and the molecular marker. According to the method, the capsicum phytophthora resistance candidate gene can be accurately identified, and the effective molecular marker can be developed.

The invention discloses a Harpin coding gene (i) hrpZPsgS1( / i) and an application of a harpinZPsgS1 protein expressed by the Harpin coding gene (i) hrpZPsgS1( / i). If the obtained harpinZPsgS1 protein is used for treating tobacco plants, the growing of the tobacco plants is promoted; and the resistance of the tobacco plants against tobacco mosaic viruses and tobacco phytophthora diseases is improved obviously, wherein the resistance is not only limited in parts treated by the harpinZPsgS1 protein but also acts on the peripheral leaves and the whole plants, and the expression of PR (pathogenesis related) protein is carried out in the treating process, so that the hrpZPsgS1( / i) can induct the plant systems to obtain disease resistance. The result shows that the hrpZPsgS1 has the application value of being developed into a biogenic pesticide.

The invention discloses effector protein Avh87 derived from phytophthora cinnamomi, and a coding gene and application thereof. The protein has the amino acid sequence of SEQ ID No. 1 in the sequence table. The nucleotide sequence expressing the protein is represented by SEQ ID No. 2 in the sequence table. Experiments show that the gene can inhibit plant cell hypersensitive reactions induced by apoptotic precursor protein Bax, or inhibit plant cell death induced by pathogenic phytophthora elicitors INF1. The effector protein Avh87 is of great significance in enriching molecular mechanism information of the interaction between the phytopathogenic oomycetes and hosts, and in establishing a comprehensive prevention and control technology strategy for phytopathogenic oomycete diseases.

The invention provides bacillus amyloliquefaciens cd5bc. The strain is classified and named as Bacillus amyloliquefaciens cd5bc and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016232, and the preservation time is April 28, 2016. The invention further provides application of the strain in the aspect of preventing and controlling crop diseases and a biocontrol preparation prepared from the strain. The strain has a very significant preventing and controlling effect on red pepper phytophthora blight, the biocontrol effect reaches up to 100% after inoculation is conducted for 15 days, and the biocontrol effect reaches up to 82.36% after inoculation is conducted for 60 days. The bacillus amyloliquefaciens cd5bc has excellent disease-resistant effects on multiple crop diseases, the bacteriostasis rates of the strain on pathogenic bacteria of red pepper sclerotinia blight, bitter gourd wilt, cucumber wilt, cucumber scabs, rice blast, a rice sheath blight disease, broad bean chocolate spots and broad bean brown spots all reach up to 70% or above, and the bacteriostasis rate of the strain on the broad bean chocolate spots and broad bean brown spots reaches up to 100%.

The present invention is in the field of soybean genetics. More specifically, the invention relates to nucleic acid molecules from regions the soybean genome, which are associated with soybean Phytophthora resistance. The invention also relates to proteins encoded by such nucleic acid molecules as well as antibodies capable of recognizing these proteins. The invention also relates to nucleic acid markers from regions the soybean genome, which are associated with soybean Phytophthora resistance. Moreover, the invention relates to uses of such molecules, including, transforming soybean Phytophthora resistant soybean with constructs containing nucleic acid molecules from regions the soybean genome, which are associated with such resistance. Furthermore, the invention relates to the use of such molecules in a plant breeding program and genetic manipulation of soybean for enhanced disease resistance.

The invention discloses a pesticide composition containing picoxystrobin and amides. The pesticide composition comprises an active component A and an active component B, wherein the active component A is selected from picoxystrobin; the active component B is selected from any one of the following bactericides: dimethomorph, metalaxyl, Metalaxyl-M, BENALAXYL, Benalaxyl-M, Flutolanil and Pyrimorph; and the weight percentage of A to B is 1%-80%:1%-80%. The composition can prevent and treat a plurality of crop diseases, has a notable synergetic action, enlarges the insecticidal spectrum, and is of high activity on downy mildew, epidemic disease, late blight, Leptosphaeria maculans, Pernophythora litchi, phytophthora, white rust, foot rot, Phytophthora parasitica, Pythium debaryanum, soft rot, damping off, banded sclerotial blight, snow mold, Leaf blight, Septoria nodorum, brown blotch or powdery mildew. The consumption of the pesticide is reduced, the residual quantity of the pesticide on crops is decreased and environmental pollution is abated.

The invention relates to a high throughput bean quarantine plant pathogenic bacteria detection method and a locking type probe. According to the detection method, the locking type probe can perform rolling circle amplification simultaneously on pea bacterial phytophthora and kidney bean dizzy phytophthora and then performs high throughput detection on the two kinds of important bean quarantine plant pathogenic bacteria. The detection method is high in specificity and sensitivity, can shorten the detection time of the bean quarantine plant pathogenic bacteria, quicken the pass speed of cargoes imported and exported in China ports, reduces enterprise costs, meanwhile can improve import bean product epidemic situation intercept rate, increase the capability of preventing foreign pest from invading, has important technicality and practicability and can generate obvious economic and social benefits.

The invention discloses a phytophthora-resistant actinomyces, which belongs to Streptomyces humidus. The phytophthora-resistant actinomyces is preserved in China Center for Type Culture Collection with an accession number of CCTCC No: M2013268. The fermentation broth of the actinomyces provided by the invention has an obvious inhibiting effect on the growth of phytophthora and is applicable to plant diseases caused by phytophthora.

The invention discloses two molecular markers in close linkage with a specific resistance gene for capsicum phytophthora blight of root rot and application thereof. Each molecular marker contains a nucleotide sequence shown in SEQ ID NO. 1 and named as P52-11-21, or contains a nucleotide sequence shown in SEQ ID NO. 2 and named as P52-11-41. The molecular markers are in close linkage with the specific resistance gene for phytophthora blight of root rot, have genetic distances of 1.5cM and 1.1cM respectively, and can be directly used for establishing an assistant breeding system for the molecular markers for capsicum phytophthora blight of root rot. The molecular markers disclosed by the invention and molecular marker amplification primers can be applied to breeding practice for capsicum phytophthora blight of root rot simply and rapidly with a high flux, and lay a good foundation for the cloning and functional researches of the specific resistance gene for capsicum phytophthora blight of root rot.

The invention discloses an S-(5-substituted-1, 3, 4-thiadiazole)-(5-substituted phenyl)-2-furancarbothioic acid ester compound, a preparation method and application thereof. The compound has a structural general formula shown as formula I. The S-(5-substituted-1, 3, 4-thiadiazole)-(5-substituted phenyl)-2-furancarbothioic acid ester compound has an obvious control effect on potato late blight, cucumber fusarium wilt, Botrytis cinerea Pers, Pepper Phytophthora blight, rice sheath blight, wheat scab, Colletotrichum musae, asparagus stem blight and the like, and can be used as an agricultural fungicide in control of plant diseases. At the same time, the compound has an inhibitory effect on HL-60 human leukemia cells, MCF-7 human breast cancer cells, BGC-823 human gastric cancer cells, Bel-7402 human hepatocellular carcinoma cells, and KB human nasopharyngeal carcinoma cells, etc., and has application prospects in the aspect of anti-tumor activity. (formula I).

The invention relates to bacillus halotolerans SY1836 and application thereof. The bacillus halotolerans SY1836 has an accession number of CGMCC No. 20080 and is a biocontrol bacterium separated and obtained from saline-alkaline soil. According to the application, verified by experiments, the bacillus halotolerans SY1836 has a relatively good effect of antagonizing various botanical pathogenic bacteria, can be used for effectively lowering a disease index of capsicum when the bacillus halotolerans SY1836 is applied to a growth process of the capsicum, and has a remarkable phytophthora blight control efficiency. The bacillus halotolerans SY1836 simultaneously can be used for remarkably improving plant height and fresh weight of the capsicum during growth and has an important significance inphytophthora blight prevention and control and planting of the capsicum.

The invention provides Bacillus amyloliquefaciens F028. The strain is preserved in the China General Microbiological Culture Collection Center on April 20, 2020, and has a preservation registration number of CGMCC No.19590. A biological control preparation prepared from the strain has a broad-spectrum antibacterial effect on bacterial diseases caused by Xanthomonas campestris, Erwiniacarotovora and Ralstonia solanacearum, and fungal diseases caused by Botrytis cinerea, Rhizoctonia solani, AlternariaNees, Fusarium, Phytophthora spp, Glomerellacingulata(Stonem.)Spauld et Schrenk, Colletotrichumcapsici and Beauveria bassiana. According to the invention, a new resource is provided for replacing a chemically synthesized bactericide with microorganisms and preventing and controlling plant diseases by using a biological prevention and control method, and the Bacillus amyloliquefaciens can be developed and utilized as a biopesticide; and moreover, the Bacillus amyloliquefaciens provided by the invention has a promotion effect on the plant growth of tomatoes and tobaccos, and has a wide application prospect.

The invention relates to an acidic soil conditioner and a preparation method thereof. The method comprises the following steps: preparation of a phosphate-containing solid from glyphosate waste liquid; preparation of a soil conditioner; granulation; drying; etc. Compared with CK, the pH value, effective phosphorus content and rapidly-available potassium content in soil of a capsicum area where the acidic soil conditioner is applied are increased by 0.44 unit, 21.60% and 33.87%, respectively; as for disease resistance, the incidence of capsicum anthracnose and the incidence of capsicum phytophthora blight are reduced by 17.83% and 26.21% compared with a control group, respectively; moreover, the contents of cadmium, lead, total mercury and total arsenic in capsicum fruit are reduced by 24.21%, 23.37%, 31.58% and 17.39%, respectively. According to the invention, recycling sustainable utilization of high-pollution waste water in production of glyphosate is realized; the pH value of acidic soil is increased; and disease resistivity and harmful heavy metal ion absorbing capacity of plants are improved.

The invention discloses a bactericidal composition and its application, the effective component comprises an ingredient A and an ingredient B. The ingredient A is metalaxyl-M, the ingredient B is selected from one of polyoxin, Ningnanmycin and validamycin. The invention has the advantages of high efficiency, safety and environmental protection. The bactericidal composition has synergistic effect which is characterized in that the product output can be raised, the cost can be reduced and the generation of drug resistance of germs can be delayed. The invention can be used for preventing and treating sheath blight, root rot, rice blast, corn northern and southern leaf blight, downy mildew, phytophthora blight, leaf spot, botrytis, alternaria leaf spot, anthracnose, ring spot, powdery mildew,black shank and late blight.

The invention relates to a bactericide composition containing fluoride ether bacteria amide and laminarin. The bactericide composition comprises a suspending agent, wettable powder, water dispersible granules and the like prepared from the fluoride ether bacteria amide, the laminarin and auxiliary agents including a wetting dispersant, a filling agent and the like. An experiment research shows that the fluoride ether bacteria amide and the laminarin are mixed according to a ratio from (10:1) to (50:1); a systemic fungicide is combined with a disease-resistant inducing agent so that the bactericide composition has an obvious synergistic effect on cucumber downy mildew, and can also delay a disease progress; the dosage of the chemical pesticide fluoride ether bacteria amide is reduced and the resistance to the fluoride ether bacteria amide by pathogenic bacteria can be effectively delayed. The bactericide composition is suitable for preventing and treating many vegetable oomycete diseases including the cucumber downy mildew, tomato phytophthora diseases, phytophthora capsici, potato late blight and the like, and can also be used for preventing and treating cotton seedling stage diseases including cotton red rot diseases and the like.

The invention relates to a suspension concentrate for seed coatings and particularly relates to a three effective component (pyraclostrobin, phenazine-1-carboxylic acid and thiamethoxam) containing compound suspension concentrate for seed coatings as well as a preparation method and application thereof, wherein the three effective component containing compound suspension concentrate is a second-generation high-efficiency low-toxicity nicotine containing insecticide, and the mass ratio of pyraclostrobin, phenazine-1-carboxylic acid and thiamethoxam is (1-80):(80-1):(1-80). The suspension concentrate has special effects, is mainly used for preventing and treating wilt, rhizoctonia solani induced blight, green sickness, damping off, rhizoctonia solani induced blight, seedling rot, sclerotinia, phytophthora diseases, dry rot, scab, sclerotial soft rot, seedling blight, stem blight, leaf blight, root rot and continuous cropping obstacle of cereals with special effects and has significant effects of preventing and treating pests such as aphids, leafhoppers, thrips, whiteflies, potato beetles and wheat bulb flies. In addition, the insecticidal and bactericidal compound suspension concentrate for seed coatings can significantly improve the insect and disease resisting capacity of spouts and seedlings and has the functions of promoting the growth and development of crop roots, strengthening seedlings and increasing the survival rate of seedlings.

The invention provides primers for detecting phytophthora cinnamomi and a nested PCR detection method. The primer group comprises two pairs of primers, wherein the first pair of primers is universal primers ph1F / Yph2R for the phytophthora Ypt1 gene, and the second pair of primers is specific primers PCINF / PCINR for identifying phytophthora cinnamomi. If the concentration reaches 10fg / [mu] L pathogenic bacteria trace DNA, phytophthora cinnamomi can be accurately detected, the nested PCR detection method for detecting phytophthora cinnamomi has the advantages that the accuracy is high, the specificity is strong, the sensitivity is high, and the detection process is simple, convenient and rapid to operate, the nested PCR detection method can be used for the early diagnosis of diseases caused by phytophthora cinnamomi and the monitoring and identifying for pathogenic bacteria, and the problem that the traditional detecting and identifying methods have complicated steps and long periods is solved.

The invention relates to an amplifying nucleic acid primer for rapidly detecting phytophthora. medicaginis at normal temperature. The LAMP amplifying nucleic acid primer comprises an FIP primer, an F3 primer, a BIP primer, a B3 primer, an LF primer and an LB primer. The LAMP detecting system of the invention has favorable specificity and can rapidly, conveniently and efficiently detect phytophthora. medicaginis under the condition of 63 DEG C and meet the rapid detecting requirement of a port on the phytophthora. medicaginis.

The invention belongs to the technical field of biological prevention and treatment, and particularly relates to a preparation method of a compound bacterium fermentation product for preventing and treating plant phytophthora, the compound bacterium fermentation product and application thereof. The preparation method comprises the following steps: (1), performing spore culturing on marine trichoderma spp., and inoculating the obtained spores into an oscillation culture medium for oscillation culture to obtain a marine Trichoderma spp. oscillation culture; (2), performing seed culturing on bacillus subtilis, inoculating the obtained seed culture into an oscillation culture medium for oscillation culture, mixing the bacillus subtilis oscillation culture with the marine trichoderma spp. oscillation culture obtained in the step (1), carrying out co-fermentation to obtain a co-fermentation product, and centrifuging and filtering the co-fermentation product to obtain a compound bacterium fermentation product. The compound bacterium fermentation product prepared by the invention remarkably reduces the growth of plant phytophthora, and has a better prevention and treatment effect on plantfungal diseases compared with the prevention and treatment effect of a single strain.

The invention discloses a coral-associated marine bacillus amyloliquefaciens strain CoMb-9 and an application thereof. The strain is preserved in the Guangdong Microbiological Culture Collection Center on Floor 5, Building 59, Courtyard 100, Xianlie Middle Road, Guangzhou, Guangdong Province on April 19, 2016, and the preservation number is GDMCC No. 60028. The strain has strong effects of inhibiting growth of plant phytophthora bacteria, plant wilt pathogenic bacteria and / or plant colletotrichum orbiculare and the like, can be colonized to propagate in soybeans, hot peppers, bananas and other crops or in rhizosphere soil of the above crops, secretes to produce antifungal proteins, indoleacetic acid and other active materials, has the effects of promoting crop growth and preventing and treating multiple plant diseases, can be applied to developing and preparing bio-organic fertilizers, biological disease prevention preparations and other agricultural biological agents with effects of promoting crop growth, improving crop yield and preventing and treating the multiple plant diseases, and has excellent popularization and application prospects in related agricultural production.

The invention provides a primer, a test kit containing the primer and a detection method for detecting phytophthora infestans, the utilization of the primer and the detection method for detecting the phytophthora infestans can realize high accuracy, strong specificity and high sensitivity, the method of the invention has the advantages of accuracy, speediness, simplicity, easy operation and the like and can identify pathogens at the early stage of infection of the disease and simultaneously detect the pathogens in field soil, and the invention has great significance for preventing and treating late bright of potatoes and tomatoes. Simultaneously, the invention also has the great significance for reducing the blind use of pesticides, reducing the production cost and reducing the environmental pollution of the pesticides.

The invention discloses a preparation method of a microbial inoculum for preventing and curing fungal diseases of plants; the microbial inoculum for preventing and curing fungal diseases of plants isprepared by mixing Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum prepared by the method of the invention is used to carry out control efficiency experiments and theresults show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and the control efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by 11.06% so that the effect for preventing diseases and increasing yield is obvious; thecontrol efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

The invention provides a polysubstituted pyrido (4, 3-d) pyrimidine compounds with bactericidal activity and a synthesis method thereof. The structural general formula of polysubstituted pyrido (4, 3-d) pyrimidine compounds of the invention is showed in general formula I which includes three compounds as showed in general formulas I-1, I-2 and I-3; the proposed structure of general formula I-1 is the development and complement of Patent CN 100335481C while no relevant coverage on the structure of the general formulas I-2 and I-3 is released currently. The experiments indicate that the compounds with the general formula I can obviously inhibit various pathogenic bacteria such as fusarium oxysporum vasinfectum, rhizoctonia solani, botrytis cinera pers, gibberella saubinetii, ring rot of apple, cotton anthracnose, cucumber timberrot, cucumber target leaf spot, scab of cucumber, pepper phytophthora blight, etc., and can be used as the active ingredient of bactericides.

The invention discloses a bactericidal composite containing dithianon and methoxy acrylic ester compounds. The sterilizing composite comprises A and B, wherein A is selected from the dithianon; B is selected from any one of the following bactericides: kresoxim-methyl, ZJ0712, pyraclostrobin and enestroburin; and the ratio of the weight parts of two active components, i.e. A and B, is (2-78: (2-78). The bactericidal composite provided by the invention can prevent and cure multiple crop diseases, has obvious synergistic action, enlarges the sterilizing spectrum, has higher activity on the diseases, i.e. downy mildew, phytophthora diseases, anthracnose, powdery mildew, alternaria mali roberts, leaf spot diseases, scab, rice blast, and the like, reduces the usage quantity of pesticides and the residual quantity of the pesticides on crops and alleviates the environmental pollution.

The invention discloses a plant source synergistic compound bactericide and an application thereof. The compound bactericide is prepared by the following steps: by taking berberine and emodin monomethyl ether as main components, adding the aids, and compounding to obtain the product. The dosage forms of the preparation refer to environment-friendly missible oil, microemulsion, aqueous solution or dissoluble liquid. The preparation comprises 0.1-20 percent of berberine, 0.1-20 percent of emodin monomethyl ether and the balance of aids. The compound bactericide does not contain any chemical pesticide component, is easy to degrade in the environment, does not have environmental pollution and residual toxins and is safe to the people, livestock and environment, and drug resistance is difficultly generated by pathogenic bacteria; the powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, tea leaf blight and other agricultural diseases on melons, fruits, vegetables and tea trees can be controlled, and the compound bactericide has extremely high application and popularization values on melons, fruits, vegetables, tea, medicinal materials and other main agricultural and sideline products exported for earning foreign exchange and is an ideal novel pollution-free bactericide.

The invention relates to the technical field of plant genetic engineering, and specifically relates to application of a gene GmLMM1 related to soybean phytophthora resistance. According to the presentinvention, the gene GmLMM1 involved in the regulation of soybean cell death, immune response and phytophthora resistance is cloned in soybean. PTI immune response and phytophthora resistance of plants can be negatively regulated by the GmLMM1 gene; and by reducing the expression of the GmLMM1 gene, PTI immune response of plant can be effectively enhanced, and phytophthora resistance of plants canbe increased. The clone and functional discovery of the GmLMM1 gene provide important gene foundations and theoretical support for the related mechanisms research of phytophthora resistance, providevaluable gene resources for advancing the research and application of plant defense systems and for breeding new soybean varieties with high disease resistance, and has important application value ingenetic engineering breeding of soybean disease resistance.

The invention discloses a hydroxyquinoline compound as well as a preparation method and application thereof in preventing and treating agricultural diseases. Testing results show that the compound hasremarkable activity upon corn leaf fulvia fulvum, cotton oxysporum, potato rhizoctonia solani kuhn, didymella bryoniae, fusarium oxysporum, wheat gibberella, rice blast, botrytis cinerea, lycium barbarum anthracnose, cotton anthracnose, sclerotinia sclerotiorum, pepper phytophthora blight, potato late blight, rhizoctonia solani, melon bacterial fruit plaque bacteria, grape meloidogynosis bacteria, Chinese cabbage erwinia carotovora, kiwi fruit anabrosis bacteria, pseudomonas lachrymans, ralstorinia solanacearum, rice bacterial leaf blight and rice bacterial stripe bacteria. The hydroxyquinoline compound is simple in preparation process, cheap and easy in raw material obtaining, high in product purity, good in bioactivity and wide in sterilization spectrum and has the potential of being developed as new fungicides.

The invention discloses chitosan oligosaccharide copper complex fungicide and application of the chitosan oligosaccharide copper complex fungicide. An active component of the fungicide is a chitosan oligosaccharide copper complex formed by the complexing of chitosan oligosaccharide and copper ions, wherein the mass ratio of the chitosan oligosaccharide to the copper ions is 5:1-1:1.2, the average molecular weight of the chitosan oligosaccharide is 161-20000, and the degree of deacetylation is greater than or equal to 85%. Experiments show that the chitosan oligosaccharide copper complex fungicide disclosed by the invention is effective in the prevention and treatment of all bacterial diseases of plants, and only can be used for preventing and treating fungal diseases, such as downy mildew, phytophthora disease, powdery mildew and cotton phytophthora disease, of the plants.