97 results about "Phytophthora" patented technology

Bacilli and combinations of Bacilli capable of germinating and growing under low temperature conditions; of associating with plant roots; of enhancing plant health, growth, and yield; and of protecting plants against phytopathogenic pests, such as Botrytis, Fusarium, Phytophthora and Pythium.

The invention relates to application of mitogen-activated protein kinase PiPmk1 in screening molecules for inhibiting formation of a phytophthora infestans infection structure. The invention finds that the molecular marker plays a key role in the development of a phytophthora infestans cyst bud tube and / or the formation of an infection structure, which indicates that the molecular marker has the potential of serving as a target for inhibiting phytophthora infestans infection, and a corresponding molecular screening method and a generative prediction model are further developed and utilized; rapid and accurate prediction and screening of phytophthora infestans infection inhibitory molecules are realized, the research and development period and cost are greatly shortened, and a new thought and a new method are provided for development of accurate targeted green pesticides.

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention discloses an ecological cultivation method for preventing pepper phytophthora blight, which comprises the following steps: step 1, soaking pepper seeds in warm water at 40-50 DEG C for 10-30 minutes, soaking for a short time to achieve the effect of softening seed coats, soaking the pepper seeds in normal-temperature water at 20-25 DEG C for 4-8 hours after soaking in warm water to ensure that the seeds absorb sufficient water, and then soaking the pepper seeds in the warm water for 10-30 minutes; soaking the chilli seeds after absorbing water in a disinfectant, thoroughly washing the seeds with clear water for 2-3 times after soaking and disinfection, and taking out the residual disinfectant; according to the ecological cultivation method for preventing pepper phytophthora blight, the disease resistance is remarkably improved through seed multi-stage treatment (gradient water temperature soaking, composite disinfection and three-bacterium coating), and the pathogen infection risk is reduced by combining seedling culture substrate bacteriostasis and a degradable non-woven fabric root protection technology; the comprehensive income is improved through application of the degradable material and basil intercropping, and finally the occurrence rate of pepper phytophthora blight is reduced.

The invention discloses a preparation method of a biochar-based bacterial fertilizer capable of relieving pepper phytophthora blight. The biochar-based bacterial fertilizer takes nutrient substances as an inner shell and beneficial microorganisms as an outer shell; the inner shell is prepared from the following components in percentage by mass: 21 to 32 percent of urea, 18 to 20 percent of monopotassium phosphate, 6 to 15 percent of potassium sulfate, 3 to 5 percent of humic acid, 3 to 8 percent of oxidized starch, 0 to 10 percent of kaolin, 0 to 10 percent of diatomite and 0 to 20 percent of charcoal; the shell is prepared from the following components in percentage by mass: 10 to 20 percent of biochar for adsorbing beneficial microorganisms and 3 to 8 percent of starch; the inner shell material and the outer shell material of the biochar-based bacterial fertilizer are uniformly mixed, 1% aminobutyric acid is sprayed for granulation, then the mixture is put into a drying oven to be dried at 45 DEG C, and finally a polyurethane coating agent is used for coating. The biochar-based bacterial fertilizer can improve the soil environment, increase the fertilizer utilization rate and reduce the occurrence of pepper phytophthora blight.

The invention discloses an endophytic subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. The strain can inhibit the growth of different physiological races of phytophthora infestans, and has inhibitory activity on various pathogenic oomycetes. The fermentation liquor of the strain contains micromolecular antibacterial substances with relatively high stability, and has remarkable antibacterial activity; protein components in fermentation liquor can induce plants to generate resistance, and infection of phytophthora infestans is effectively inhibited. Different types of microbial agents prepared by utilizing the strain DS1 can be used for effectively preventing and treating potato late blight, and the microbial preparation is used for seed dressing treatment of potatoes and wheat and can be used for remarkably promoting the growth of crops.

This invention discloses the CAPE1 peptide at the C-terminus of the PtPR1 protein and its applications. There are three types of CAPE1 peptides in the PtPR1 protein: PtPR1f (amino acid sequence shown in SEQ ID NO.1), PtPR1j (amino acid sequence shown in SEQ ID NO.2), and PtPR1l (amino acid sequence shown in SEQ ID NO.3). Experimental results show that the three CAPE1 peptides at the C-terminus of the PtPR1 protein provided by this invention can induce an immune defense response in woody plants at low concentrations. The use of these three CAPE1 peptides can also significantly improve the disease resistance of woody plants to *Colletotrichum gloeosporioides*, *Pseudomonas aeruginosa*, *Botrytis cinerea*, or *Phytophthora spp.*, without inhibiting the growth of these pathogens. Therefore, it can be applied to the prevention and control of woody plant diseases, providing a new approach to improving the disease resistance of woody plants and showing broad application prospects in the green control of woody plant diseases.

The application discloses application of a fungal virus in prevention and treatment of pear tree and apple tree Valsa diseases. The fungal virus is a positive single-stranded RNA virus, and a cDNA nucleotide sequence of the fungal virus is shown as SEQ ID No. 1. A fungus containing the virus is Valsa pyri HJL1-22 (gamma), the fungus is infected by the fungal virus of claim 1, the fungus is named as HJL1-22 (gamma), is classified and named as Valsa pyri, and has a preservation number of CGMCC No. 41449 in China General Microbiological Culture Collection Center. The virus Vphv1-gamma can be secreted to outside of cells, therefore, culture liquid filtered off mycelium can be used as a biocontrol agent for prevention and treatment of pear tree and apple tree Valsa diseases, and can effectively weaken or inhibit pathogenicity and diffusion of Valsa pyri and Valsa mali, and has a prospect for being used for producing and practicing biological prevention and treatment of pear tree and apple tree Valsa diseases.

The invention discloses a bis (N-acylamino-3, 1-benzoxazinyl) methane compound and a preparation method and application thereof.The compound takes an arylamine compound and bromoacetyl bromide as raw materials, triethylamine as an acid-binding agent and dichloromethane as a solvent to react in an ice-water bath to obtain an N-aryl-2-bromoacetyl arylamine compound, then the N-aryl-2-bromoacetyl arylamine compound is subjected to multi-step reaction, and the bis (N-acylamino-3, 1-benzoxazinyl) methane compound is obtained. Finally, the bis (N-acylamino-3, 1-benzoxazinyl) methane compound with bactericidal activity is obtained. The raw materials for synthesis of the compound are cheap and easy to obtain, and the synthesis method is simple. Meanwhile, the compound has a good inhibition effect on the activity of crop germs, especially has remarkable inhibition activity on sclerotinia sclerotiorum, phytophthora, gibberellic disease bacteria, magnaporthe oryzae, botrytis cinerea and rhizoctonia solani, and has remarkable application value in the aspects of crop disease control and drug resistance challenge.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

The present invention relates to bio-fungicides that inhibits Phytophthora infestans. More particularly, the present invention relates to novel strains of Bacillus spp. that can inhibit Phytophthora infestans. The present invention also relates to bio-fungicidal formulations comprising novel strains of Bacillus spp. either alone or a mixture of novel strains of Bacillus spp., in combination with other excipients.

The invention discloses a 3-ester-1, 3-quinazoline-2, 4-diketone compound and a preparation method and application thereof.The 3-ester-1, 3-quinazoline-2, 4-diketone compound is prepared by the steps that amino-acid ester hydrochloride and isatoic anhydride serve as raw materials and react in acetonitrile under the action of potassium carbonate to generate an amide ester compound; and then reacting the amide ester compound with triphosgene under the action of triethylamine to obtain the 3-ester-1, 3-quinazoline-2, 4-diketone compound. Raw materials for synthesis of the compound are cheap and easy to obtain, the synthesis method is simple, meanwhile, the compound has good bacteriostatic activity on crop germs, especially has a remarkable activity inhibition effect on germs such as gibberellic disease bacteria, phytophthora germs, pyricularia grisea, sclerotinia sclerotiorum, botrytis cinerea and rhizoctonia solani, and the yield of crops is well guaranteed.

The invention provides application of a DTX29 gene and a protein thereof in improving the resistance of plants to phytophthora infestans. The invention finds a susceptible gene DTX29 capable of effectively improving the resistance of plants to phytophthora infestans through a large number of researches. The DTX29 gene of the plant is edited through a CRISPR-Cas technology and the like, so that the function of the DTX29 gene is lost, the resistance of the plant to phytophthora infestans can be effectively improved, the diameter and area of disease spots and the relative biomass of phytophthora infestans are reduced, and normal growth and development of the plant are not influenced. The invention provides a new target and idea for prevention and control of plant diseases caused by phytophthora infestans.

The invention belongs to the technical field of biology, and particularly relates to a denitrifying hyphomicrobium high-producing strain for producing pyrroloquinoline quinone (PQQ) and application of the denitrifying hyphomicrobium high-producing strain. In particular, the present invention provides an isolated denitrifying hyphomicrobium strain comprising a 16S rDNA sequence having at least 96% identity with one or more of SEQ ID NO: 1. The invention further provides a separated denitrifying hyphomicrobium strain, and the separated denitrifying hyphomicrobium strain comprises the denitrifying hyphomicrobium strain SHYZPQQ001 with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252085, namely the denitrifying hyphomicrobium strain SHYZPQQ001.

In one aspect, the present application discloses compositions of extracellular vesicles (EVs) comprising heterologous effector RNAs, such as antifungal RNAs. In certain embodiments, the effector RNAs comprise dsRNA, while in other embodiments the effector RNAs comprise mRNA In certain embodiments, the EVs are plant EVs, microbial EVs (e.g., bacterial EVs or fungal EVs), or artificial EVs. In particular embodiments, the EVs are plant EVs and the effector RNA is mRNA. In other particular embodiments, the EVs are microbial EVs and the effector RNA is dsRNA. In other aspects, the application discloses microbes that produce RNA-loaded EVs; methods of treating and / or preventing fungal diseases by applying such microbes and / or RNA-loaded EVs to plants, plant parts, and / or soil; and methods of producing such RNA-loaded EVs. In certain examples, the antifungal RNAs may target DCL1 / 2 genes the VDS genes, and / or the tetraspanin (PLS1) genes of a fungal pathogen such as B. cinerea, Verticillium dahliae, Sclerotinia sclerotiorum, Fusarium oxysporum, Fusarium graminearum, Aspergillus niger or even oomycete pathogens, such as Phytophthora infestans.

The present application provides a kind of compound biocontrol agent and its preparation method and application technology, and it is composed of 1000ml of fermentation mixed liquor of multiple strains of biocontrol bacteria mixed in equal volume ratio and 0.5%-1.5% stabilizer with complementary mechanism and target.The fermentation concentrated solution of multiple strains of bacteria is prepared in fermentation medium to obtain the present application, which is used for preventing and controlling fusarium, pythium, phytophthora, rhizoctonia solani, ralstonia solanacearum, erwinia carotovora, streptomyces scabiei, xanthomonas campestris and other plant pathogenic bacteria and nematodes, which can inhabit in soil for a long time and spread through soil, and one or several of them can cause plant soil-borne diseases.The preparation process of the present application is not affected by too many chemical and physical factors, the activity of live bacteria and metabolites is high, the average control effect is 78%-85%, the application effect is good;The production process is simple, energy and auxiliary materials are saved, and the production cost is low.

The invention discloses bacillus siamensis and application thereof, and relates to the technical field of microorganisms. The strain is bacillus siamensis LYQ15 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 14, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO: 30024. The strain provided by the invention can be used for effectively inhibiting the growth and reproduction of phytophthora and pseudomonas solanacearum as well as related diseases caused by the growth and reproduction of the phytophthora and the pseudomonas solanacearum; in addition, the bacillus siamensis LYQ15 can inhibit reproduction of pathogenic bacteria and epidemic of diseases in an environment with the pathogenic bacteria, and healthy growth of tobacco plants is guaranteed.

The present invention discloses the application of alfalfa VIK protein in cultivating plants resistant to Phytophthora root rot, and belongs to the field of biotechnology. The technical problem solved by the present invention is how to improve the disease resistance of plants. The VIK protein disclosed in the present invention is a protein whose amino acid sequence is SEQ ID No.1 or SEQ ID No.4. Experiments have shown that after inoculation with Phytophthora plant pathogens, compared with wild-type plants, the mutants obtained by editing the VIK encoding gene have enhanced disease resistance and improved survival rate, increased plant fresh weight, above-ground part height, root length, and plant height, and decreased leaf relative conductivity. Therefore, mutations in the VIK protein and its encoding gene improve the plant's resistance to Phytophthora plant pathogens. The VIK encoding gene can be targeted by gene editing technology to obtain VIK gene knockout plants and cultivate plants resistant to Phytophthora plant pathogens.

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.

The application belongs to the technical field of bio-agriculture, and particularly relates to a gene MOS4 and application of an encoded protein thereof in regulation of plant resistance to parasitic Phytophthora. MOS4 Through gene function research, it is found that overexpression of the gene MOS4 significantly improves the plant resistance to parasitic Phytophthora, and that the expression of the gene MOS4 weakens the plant resistance to parasitic Phytophthora, and that the gene MOS4 positively regulates the plant resistance to parasitic Phytophthora. Further immune mechanism research finds that the gene MOS4 regulates the related genes of a salicylic acid (SA) signal path, interacts with a transcription factor ERF019, and jointly activates the plant immune response. In addition, a dual luciferase experiment proves that the gene MOS4 and the gene ERF019 cooperatively regulate the transcription of PR1. The application provides a new molecular target for prevention and treatment of plant diseases caused by parasitic Phytophthora, and has important agricultural application value.

The invention discloses a fungus strain 41233 from Western sand and application of the fungus strain 41233, the taxonomic name of the fungus strain 41233 is Plectosphaerella sp., the preservation number of the fungus strain 41233 is GDMCC No.67505, and the preservation date of the fungus strain 41233 is December 19, 2025. The invention further discloses application of the fungus strain 41233 and a crude extract of the fungus strain 41233 in preparation of pathogenic bacterium inhibition products. The fungus strain 41233 sourced from Xiaxa has inhibitory activity on mango colletotrichum gloeosporioides and / or phytophthora capsici, and a crude extract of the fungus strain 41233 sourced from Xiaxa has inhibitory activity on various pathogenic bacteria such as colletotrichum gloeosporioides, phytophthora and fruit rot pathogens, and can be used for preparing biocontrol inoculants or further mining active natural products. And a new green prevention and control means is provided for plant disease prevention and control.

The application belongs to the technical field of molecular biology breeding, and particularly relates to application of RIN4 family genes in improving resistance of tomatoes to Phytophthora infestans. Two Phytophthora infestans disease-related genes are found and verified in tomatoes, the genomic nucleotide sequences of which are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes both belong to members of the RIN4 gene family. By knocking out or silencing the above genes through gene editing technology, it is found that the tomato lines with the above genes knocked out or silenced have higher resistance to Phytophthora infestans compared with wild types. Meanwhile, the gene-edited tomato lines are not obviously affected in growth and development. Therefore, the application not only enriches the research on Phytophthora infestans disease-related genes in tomatoes, but also develops tomato lines with enhanced resistance to Phytophthora infestans by using the characteristics of the above genes, thereby providing new technical strategies and gene resources for tomato breeding.

The present invention provides a Bacillus megaterium LDSR-6 bacterium agent for producing lipopeptide antibiotics and its application. The Bacillus megaterium LDSR-6 of the present invention has a preservation number of CGMCC No. 30083. The Bacillus megaterium of the present invention has antibacterial activity against various plant pathogenic bacteria such as Fusarium, has strong salt tolerance and no hemolysis; the Bacillus megaterium LDSR-6 bacterium agent has a preventive effect of 62.31% on Astragalus root rot under greenhouse conditions, and the lipopeptide antibiotics produced have a broad-spectrum and high-efficiency antibacterial effect on various pathogenic bacteria such as Fusarium and Phytophthora, and can cause the infection hyphae of various Fusarium to be deformed. The Bacillus megaterium of the present invention is a biocontrol strain with excellent effects and has important significance for the biological control of plant diseases.

The invention belongs to the technical field of soil microorganism regulators, and particularly relates to a pepper continuous cropping obstacle resistant soil microorganism regulator which is composed of biocontrol bacterium components and compound factors. The biocontrol bacterium component is bacillus amyloliquefaciens PQ21 with 10 billion spores per gram; the compound factor is chrysophanol. Soil microorganism bacillus amyloliquefaciens PQ21 is selected as a biocontrol bacterium component of the regulator, and chrysophanol is selected as a compound factor. Indoor experiments find that when the bacillus amyloliquefaciens PQ21 and chrysophanol are combined for use, the bacillus amyloliquefaciens PQ21 and chrysophanol have a synergistic effect on resisting phytophthora capsici and pepper root knot nematode disease, the prevention and treatment effect on the phytophthora capsici and pepper root knot nematode disease can be improved, and support can be provided for developing eco-friendly and sustainable medicaments for preventing and treating pepper diseases.

This invention belongs to the field of bio-agricultural technology, specifically relating to genes. DISC1 The application of its encoded proteins in regulating plant resistance to Phytophthora parasiticans. This invention, through the study of... DISC1 Studies on the function of genes and their encoded proteins have revealed that... DISC1 Negative regulation of plant resistance to Phytophthora parasiticum. Overexpressed genes. DISC1 Significantly reduces plant resistance to Phytophthora parasiticum, gene DISC1 The mutant plants exhibited significantly enhanced resistance to Phytophthora parasiticum. Further research into the immune mechanism revealed that the gene... DISC1 This invention regulates plant immune responses by modulating genes related to the jasmonic acid (JA) signaling pathway. It provides a novel gene resource for the control of plant resistance to Phytophthora parasiticum.

The invention belongs to the technical field of gene engineering, and relates to application of a potato StEDR1 gene in regulation and control of late blight resistance. The potato StEDR1 gene is obtained, and the effect of the potato StEDR1 gene in regulation and control of potato late blight resistance is disclosed. According to the invention, through overexpression and gene editing technologies, the Benshi tobacco leaf of the overexpressed StEDR1 gene shows higher susceptibility to phytophthora infestans, and the hairy root of the potato after the StEDR1 gene is targeted by a CRISPR / Cas9 carrier shows a resistance-enhanced phenotype. The invention provides more strategies and ideas for enriching anti-susceptible gene resources and creating a persistent late blight disease-resistant material.

The invention relates to the technical field of biology, in particular to a defensive protein derived from konjak and having broad-spectrum antibacterial activity, a coding gene and application of the defensive protein and the coding gene. According to the invention, the defensive protein is obtained from konjak for the first time, and the defensive protein has an obvious inhibition effect on multiple fungi (rhizoctonia solani, fusarium oxysporum, fusarium aromatic, fusarium solani and phytophthora nicotianae) and bacteria (bacillus cereus and ralstonia solanacearum). The defensive protein has wide application prospects in the fields of food, hygienic products, cosmetics, biopesticides, biological feed additives or natural food preservatives and the like.

The application discloses a tobacco protein disulfide isomerase NbPDI and application thereof in regulation of plant immunity. The application obtains an anti-disease related gene NbPDI01 and a susceptible gene NbPDI23 from tobacco. NbPDI01 and NbPDI23 are overexpressed in tobacco through a transient expression technology, and pepper Phytophthora is inoculated after 2 days. The results show that, compared with a negative control, the disease spot area of the tobacco leaf expressing NbPDI01 is significantly reduced, while the disease spot area of the tobacco leaf expressing NbPDI23 is significantly increased, and biological statistics analysis shows that the difference between the two is significant. It is shown that NbPDI01 can specifically improve the disease resistance of tobacco, and therefore, the NbPDI01 gene provided by the application can be applied to the fields of plant disease resistance breeding, disease resistance gene synthesis and genetic engineering improvement, and provides a new gene resource for improving the disease resistance of crops, and has important theoretical significance and application prospect.

The invention discloses a Trichoderma guasei YZU-TG1 strain and application of the Trichoderma guasei YZU-TG1 strain. According to the invention, it is found for the first time that the Trichoderma guasei YZU-TG1 strain can inhibit growth of phytophthora and fungi, and has broad-spectrum antibacterial ability; according to the present invention, the produced main volatile matters can effectively prevent and control post-harvest potato late blight, such that the novel biocontrol strain is hopeful to provide for the prevention and control of the crop epidemic disease, can be developed into the biocontrol bacterial agent and the biological bacterial fertilizer, can be used for the green prevention and control of the crop epidemic disease, and has broad prospects. The strain drug resistance problem caused by excessive use of chemical pesticides is solved to a certain extent, and the strain has a wide application prospect in safe production of crops.

The invention belongs to the technical field of molecular biology breeding, and particularly relates to application of PP2C family genes in improving resistance of tomatoes to phytophthora infestans. Two genes related to phytophthora infestans infection are found and verified in tomatoes, the genome nucleotide sequences of the two genes are shown as SEQ ID NO: 1 and SEQ ID NO: 2 respectively, and the two genes belong to PP2C gene family members. The gene is knocked out or silenced through a gene editing technology, and it is found that the tomato strain with the gene knocked out or silenced has higher phytophthora infestans resistance compared with a wild type. Meanwhile, tomato strains edited by the genes are not obviously influenced in the aspects of growth and development. Therefore, the invention not only enriches the research of phytophthora infestans susceptibility related genes in tomatoes, but also develops a tomato strain with enhanced phytophthora infestans resistance by utilizing the characteristics of the genes, and provides a new technical strategy and gene resources for tomato breeding.