13 results about "Phytophthora" patented technology

This invention belongs to the field of biological agent technology, specifically relating to a strain of *Trichoderma*, a biocontrol agent, a bio-organic fertilizer, and methods and applications for controlling plant diseases. This invention provides a strain of *Trichoderma* P11, with accession number CCTCC NO: M 2024648. The *Trichoderma* P11 of this invention can enhance positively correlated disease resistance genes in plants. PnCAD , PnSN1 , PnDEFL1 The relative expression level of *Trichoderma* P11 was increased, enhancing the activity of disease resistance-related enzymes SOD, POD, and CAT. Furthermore, *Trichoderma* P11 could damage the cell membrane of pathogens, inhibiting their growth. *Trichoderma* P11 also exhibits good rhizosphere colonization ability. The results of the examples show that *Trichoderma* P11 of the present invention can reduce the incidence of root rot in Panax notoginseng fields and can also antagonize various pathogens such as *Pseudomonas aeruginosa*, *Pseudomonas oryzae*, *Geotrichum candida*, *Early Blight of Tomato*, *Phytophthora indicum*, *Phytophthora capsici*, and *Alternaria spiralis*.

The application discloses a tobacco protein disulfide isomerase NbPDI and application thereof in regulation of plant immunity. The application obtains an anti-disease related gene NbPDI01 and a susceptible gene NbPDI23 from tobacco. NbPDI01 and NbPDI23 are overexpressed in tobacco through a transient expression technology, and pepper Phytophthora is inoculated after 2 days. The results show that, compared with a negative control, the disease spot area of the tobacco leaf expressing NbPDI01 is significantly reduced, while the disease spot area of the tobacco leaf expressing NbPDI23 is significantly increased, and biological statistics analysis shows that the difference between the two is significant. It is shown that NbPDI01 can specifically improve the disease resistance of tobacco, and therefore, the NbPDI01 gene provided by the application can be applied to the fields of plant disease resistance breeding, disease resistance gene synthesis and genetic engineering improvement, and provides a new gene resource for improving the disease resistance of crops, and has important theoretical significance and application prospect.

This invention discloses the effector protein *PpiniAvh17* from *Phytophthora indicum*, its encoding gene, and its applications, belonging to the field of molecular biology. The amino acid sequence of this protein is shown in SEQ ID NO.1. Experiments have demonstrated that this protein can effectively inhibit programmed cell death (PCD) induced by pathogen effector proteins (such as *Phytophthora indicum* INF1) and can promote *Phytophthora capsici* infection in plants, indicating that it functions as a toxic effector to suppress plant immunity. This invention also provides the gene encoding this protein (SEQ ID NO.2). This protein and its encoding gene can be used to prepare products that regulate plant cell death, enhance plant stress resistance, or study plant-pathogen interaction mechanisms, providing new molecular targets and tools for plant disease control.

The application discloses a flavonol derivative containing piperazine-isopropyl alcohol amine and a preparation method and application thereof, and belongs to the technical field of pesticide synthesis. The application introduces piperazine-isopropyl alcohol amine with excellent activity into the structure of flavonol, and synthesizes a series of flavonol derivatives containing piperazine-isopropyl alcohol amine. Through activity test of the synthesized compounds on inhibition of plant pathogenic bacteria, it is found that the synthesized flavonol derivatives containing piperazine-isopropyl alcohol amine can effectively inhibit plant pathogenic bacteria, especially pepper phytophthora blight bacteria ( P.c ) and rice bacterial leaf blight bacteria ( Xoo ).

Described are CRISPR constructs and systems that can be used to generate Phytophthora crown rot resistant strawberry cultivars rapidly and efficiently.

The application discloses an indole derivative containing benzothiazole and pyridine as well as a preparation method and application thereof, and belongs to the technical field of pesticide synthesis. The application introduces benzothiazole and pyridine groups with excellent activity into the structure of indole, and synthesizes a series of indole derivatives containing benzothiazole and pyridine. Through inhibition of plant pathogenic bacteria activity test on the synthesized indole derivatives containing benzothiazole and pyridine, it is found that the synthesized indole derivatives containing benzothiazole and pyridine can effectively inhibit plant pathogenic fungi, especially pepper phytophthora blight.

This invention discloses a 1-(2-aminobenzyl)-3-arylurea compound, its preparation method, and its uses. This type of compound is synthesized from o-aminobenzylamine and aryl isocyanate compounds as raw materials, using triethylamine as an acid-binding agent and dichloromethane as a solvent via a condensation reaction in an ice-water bath. The raw materials used in the synthesis of this type of compound are inexpensive and readily available, and the synthesis method is simple. It exhibits good inhibitory effects on crop pathogens, particularly showing significant inhibitory activity against Sclerotinia sclerotinia, Phytophthora blight, Fusarium head blight, Carbendazim, Botrytis cinerea, and Sheath blight.

The invention discloses a phytophthora pini effector protein PpinAvh23 as well as a coding gene and application thereof, and belongs to the technical field of molecular biology. The amino acid sequence of the protein is as shown in SEQ ID NO. 1. Experiments show that the protein can specifically inhibit programmed death of plant cells induced by pathogenic bacteria elicitors, and it is proved that the protein can promote pathogen infection as a toxic effector in plants. The invention also provides a gene (SEQ ID NO.2) for coding the protein. The protein and the coding gene thereof can be used as a key molecular tool and a screening target for researching a plant-pathogen interaction mechanism, and a basis is provided for developing a new strategy for preventing and controlling plant diseases.

The present application relates to a kind of arylamino-containing xanthine derivatives and its preparation method and application.On the basis of 1,3-disubstituted xanthine raw material, arylamino-containing xanthine derivatives obtained by series of reactions are shown as general formula I.Compounds of general formula I have higher herbicidal activity, and have obvious activity on rape, amaranthus retroflexus, barnyard grass, crabgrass, have good insecticidal activity, especially on the effect of diamondback moth is remarkable, have good fungicidal activity, and have obvious in vitro inhibitory activity on wheat scab fungus, pepper phytophthora blight fungus, sclerotinia sclerotiorum, cucumber fusarium wilt fungus, apple phialophora quercina, wheat sharp eyespot fungus, etc.Compounds of general formula I can be used as active components of herbicidal or insecticidal or fungicidal composition, for preparing agricultural herbicide or insecticide or fungicide, and can be used for the prevention and treatment of grass damage or insect damage or pathogenic damage on various crops.Also have good inhibitory effect on the activity of KARI, and can be used for KARI inhibitor.

Bacilli and combinations of Bacilli capable of germinating and growing under low temperature conditions; of associating with plant roots; of enhancing plant health, growth, and yield; and of protecting plants against phytopathogenic pests, such as Botrytis, Fusarium, Phytophthora and Pythium.

This invention discloses an N-aryl(2-acylaminobenzoyl)aminoamide compound, its preparation method, and its uses. This compound is generated by reacting Boc-amino acids and aromatic amines in dichloromethane under the action of EDCI, DMAP, and triethylamine. The N-Boc-aminobenzoyl compound is then acid-hydrolyzed with trifluoroacetic acid to obtain an aminobenzoyl compound. Next, the aminobenzoyl compound is reacted with indomethacin anhydride in acetonitrile under the action of potassium carbonate to generate a diamide compound. Finally, the diamide compound is reacted with an acyl halide in the action of triethylamine to obtain the N-aryl(2-acylaminobenzoyl)aminoamide compound. The raw materials used in the synthesis are inexpensive and readily available, and the synthesis method is simple. This compound exhibits good inhibitory activity against crop pathogens, particularly against Sclerotinia sclerotinia, Phytophthora blight, Fusarium head blight, rice blast fungus, gray mold, sheath blight, and rot fungus.

This invention discloses genes PlSWL1 And its application in the prevention and control of downy mildew in litchi. This invention constructs... PlSWL1 The gene was knocked out via vector and transformed into a protoplast using PEG-mediated homologous recombination. PlSWL1 Knockout mutants and replacement transformants were obtained from *Phytophthora licoricei* through knockout and replacement. Compared with the wild type, the mutants showed a significantly reduced germination rate of dormant spores and a markedly weakened pathogenicity of zoospores and hyphae. This invention confirms... PlSWL1 It is essential for the growth, development, infection, and pathogenicity of Phytophthora lichei, and helps to elucidate the pathogenic molecular mechanism of Phytophthora lichei, providing a target gene for the development of effective fungicides.