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Method for preventing and curing fungal diseases of plants

A plant fungal disease and fungal agent technology, applied in the field of plant fungal disease prevention and control, can solve the problems of destroying the structure of biological communities, killing beneficial microorganisms, and residual harmful substances in vegetables

Inactive Publication Date: 2010-02-03
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term, repeated and large-scale use of chemical pesticides will lead to the killing or inhibition of beneficial microorganisms, destroy the structure of biological communities, cause environmental pollution, and cause a large number of harmful substances in vegetables to remain, leading to the development of drug resistance or drug resistance of pathogenic bacteria, thus making chemical pesticides The increased use of the

Method used

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  • Method for preventing and curing fungal diseases of plants
  • Method for preventing and curing fungal diseases of plants
  • Method for preventing and curing fungal diseases of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the preparation of the bacterial agent of prevention and treatment plant fungus disease and its control effect experiment

[0020] 1. Preparation of liquid inoculant for preventing and controlling plant fungal diseases

[0021] Screening medium for chlamydospore production: KH 2 PO 4 2.0g / L, NH 4 NO 3 1.0g / L, FeSO 4 .7H 2 O 0.005g / L, MnSO 4 0.0016g / L, CaCl 2 0.3g / L, NaCl 1.0g / L, MgSO 4 .7H 2 O 0.3g / L, corn stalk powder (through 40mm sieve) 10g / L. PH 6.0. Sterilize at 115°C for 20 minutes.

[0022] Trichoderma longhandle ACCC 30150 and Trichoderma lime viridae ACCC 30152 were inoculated into PDA liquid medium respectively, and cultured on a shaker at 30° C. for 3 days (shaker speed 265 rpm). Then according to the inoculum size of 10% (volume percentage composition), inoculate in the above-mentioned screening and produce chlamydospore culture medium, 30 ℃ of shaking table cultures (shaking table rotating speed 250 rev / mins) to the growth of Tric...

Embodiment 2

[0070] Embodiment 2, the preparation of the bacterial agent of prevention and treatment plant fungus disease and control effect experiment thereof

[0071] 1. Preparation of liquid inoculant for preventing and controlling plant fungal diseases

[0072] Screening medium for chlamydospore production: KH 2 PO 4 2.0g / L, NH 4 NO 3 1.0g / L, FeSO 4 .7H 2 O 0.005g / L, MnSO 4 0.0016g / L, CaCl 2 0.3g / L, NaCl 1.0g / L, MgSO 4 .7H 2 O 0.3g / L, corn stalk powder (through 40mm sieve) 10g / L. PH 6.0. Sterilize at 115°C for 20 minutes.

[0073] Trichoderma longhandle ACCC 30150 and Trichoderma lime viridae ACCC 30152 were inoculated into PDA liquid medium respectively, and cultured on a shaker at 30° C. for 3 days (shaker speed 265 rpm). Then according to the inoculum size of 10% (volume percentage composition), inoculate in the above-mentioned screening and produce chlamydospore culture medium, 30 ℃ of shaking table cultures (shaking table rotating speed 250 rev / mins) to the growth of ...

Embodiment 3

[0115] Embodiment 3, the preparation of the bacterial agent of preventing and treating plant fungus disease and its control effect experiment

[0116] 1. Preparation of liquid inoculant for preventing and controlling plant fungal diseases

[0117] Screening medium for chlamydospore production: KH 2 PO 4 2.0g / L, NH 4 NO 3 1.0g / L, FeSO 4 .7H 2 O 0.005g / L, MnSO 4 0.0016g / L, CaCl 2 0.3g / L, NaCl 1.0g / L, MgSO 4 .7H 2 O 0.3g / L, corn stalk powder (through 40mm sieve) 10g / L. PH 6.0. Sterilize at 115°C for 20 minutes.

[0118] Trichoderma longhandle ACCC 30150 and Trichoderma lime viridae ACCC 30152 were inoculated into PDA liquid medium respectively, and cultured on a shaker at 30° C. for 3 days (shaker speed 265 rpm). Then according to the inoculum size of 10% (volume percentage composition), inoculate in the above-mentioned screening and produce chlamydospore culture medium, 30 ℃ of shaking table cultures (shaking table rotating speed 250 rev / mins) to the growth of Tric...

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Abstract

The invention discloses a preparation method of a microbial inoculum for preventing and curing fungal diseases of plants; the microbial inoculum for preventing and curing fungal diseases of plants isprepared by mixing Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum prepared by the method of the invention is used to carry out control efficiency experiments and theresults show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and the control efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by 11.06% so that the effect for preventing diseases and increasing yield is obvious; thecontrol efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

Description

technical field [0001] The invention relates to a method for preventing and treating fungal diseases of plants. Background technique [0002] Since the 1980s, my country's vegetable planting industry has developed rapidly, and vegetables have become the second largest crop after food crops. Due to the special environment of vegetable cultivation, suitable conditions are provided for the occurrence and development of vegetable diseases, resulting in an increasing trend in the types, quantity and degree of damage of vegetable diseases year by year, seriously affecting the yield and quality of vegetables, and causing great harm to vegetable farmers. The huge economic loss has become a "bottleneck" that restricts the development of my country's vegetable planting industry and enhances the competitiveness of the international market. The prevention and control of vegetable diseases is a necessity for the development of vegetable planting industry in my country, and it is also an ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N3/00
Inventor 姜瑞波李世贵顾金刚张晓霞阮志勇马晓彤张瑞颖
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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