Patents
Literature
Patsnap Eureka AI that helps you search prior art, draft patents, and assess FTO risks, powered by patent and scientific literature data.

69 results about "Phytophthora sp." patented technology

Phytophthora spp. are mostly pathogens of dicotyledons, and many are relatively host-specific parasites. Phytophthora cinnamomi, though, infects thousands of species ranging from club mosses, ferns, cycads, conifers, grasses, lilies, to members of many dicotyledonous families.

Mortierella alpina strain and application thereof in plant growth promotion, disease prevention and soil improvement

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

A gene GmCDK8 for negatively regulating soybean resistance to Phytophthora spp. and its application

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.
Owner:NANJING AGRICULTURAL UNIVERSITY

Gene GmCDK8 for negative regulation of phytophthora sojae resistance and application of gene GmCDK8

The invention discloses a gene GmCDK8 for negative regulation and control of phytophthora sojae resistance and application of the gene GmCDK8. GmCDK8-knocked-out soybean plants disclosed by the invention enhance the resistance of soybeans to phytophthora sojae; the results of root scab length, leaf scab area, phytophthora accumulation amount, leaf infected and necrotic areas and the like of the soybean cultivar Williams 82 without the GmCDK8 under the stress of phytophthora show that the resistance of the plant to the phytophthora can be remarkably improved by knocking out the GmCDK8. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.
Owner:NANJING AGRICULTURAL UNIVERSITY

Bactericidal composition containing fluxapyroxad and thiediazole copper

InactiveCN107771825ASynergistic effect is obviousDelay key drug resistanceBiocideFungicidesBacterial compositionPhytophthora sp.
The invention relates to a bactericidal composition containing fluxapyroxad and thiediazole copper. The bacterial composition is compounded from fluxapyroxad and thiediazole copper in a binary mode, wherein a mass ratio of fluxapyroxad to thiediazole copper is (1-50) to (50-1); a mass fraction of fluxapyroxad and thiediazole in the preparation is 1%-80%, and the rest of the preparation is auxiliary components which are allowed to use and are acceptable in a pesticide. A dosage form of the bactericidal composition is missible oil, a suspending agent, wettable powder, a water dispersible granule, emulsion in water, microemulsion, a granule and a micro-capsule. The bactericidal composition is mainly used for controlling plant gray mold, peronospora and phytophthora germs.
Owner:HAILIR PESTICIDES & CHEM GRP

Application of demethylzeylasteral in preparation of product for preventing and treating phytophthora

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.
Owner:HAINAN UNIVERSITY SANYA NANFAN RESEARCH INSTITUTE +1

Application of CaBBX2 gene in improving resistance of plants to epidemic diseases

The invention provides an application of a CaBBX2 gene in improving the resistance of plants to epidemic diseases, and belongs to the field of plant genetic engineering. The invention finds that overexpression of CaBBX2 can have an obvious inhibition effect on phytophthora of invasive plants. The method provides a new technical approach for plant disease-resistant breeding.
Owner:JIANGXI AGRICULTURAL UNIVERSITY

Application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.
Owner:SANYA NATIONAL INSTITUTE OF SOUTHERN BREEDING CHINESE ACADEMY OF AGRICULTURAL SCIENCES +2

Bacillus velezensis XZT106 and application thereof

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.
Owner:HUNAN INST OF MICROBIOLOGY +1

Use of 5,8,11-eicosatrienoic acid in the prevention and treatment of plant fungal diseases

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.
Owner:HUAZHONG AGRI UNIV

Use of a sesquiterpene synthase and mutants thereof in the synthesis of drimane compounds

PendingCN122445617AEscherichia coliHeterologous
The application belongs to the field of enzyme genetic engineering, and particularly relates to application of a sesquiterpene synthase and a mutant thereof in synthesis of enantiomeric-drimenol compounds, wherein the sesquiterpene synthase is a sesquiterpene synthase from Chenopodium quinoa Willd., and includes CqDMDS1 or CqDMDS2; the mutant of the sesquiterpene synthase is a mutant CqDMDS1 in which an amino acid at position 443 of CqDMDS1 is mutated F443A / G The application realizes heterologous expression of the sesquiterpene synthase and the mutant thereof from Chenopodium quinoa Willd. in an Escherichia coli and a N. benthamiana expression system, catalyzes synthesis of (-)‑8,11-driman-diol or (+)‑drimenol. It is found through activity test that the sesquiterpene synthase from Chenopodium quinoa Willd. has a significant inhibitory effect on plant pathogenic fungi Phytophthora nicotianae, Phytophthora sojae and Fusarium graminearum. The sesquiterpene synthase and the mutant thereof and the biological preparation of the catalytic product have important significance for development of biological pesticides.
Owner:SOUTHERN UNIVERSITY OF SCIENCE AND TECHNOLOGY

A thiophene-based agricultural fungicide

PendingCN122444771ABiotechnologySporeling
The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.
Owner:CHINA AGRI UNIV

Strain R3 of spathiphyllum conifer and complex microbial inoculant thereof, and application of strain R3 and complex microbial inoculant in prevention and control of late blight

PendingCN121574869ABiocideBacteriaBiotechnologySpathula
The invention provides a spathiphyllum conifer strain R3, a complex microbial inoculant thereof and application of the spathiphyllum conifer strain R3 in prevention and control of late blight, and belongs to the technical field of biocontrol bacteria. The invention relates to a spathiphylli strain R3, and the preservation number of the spathiphylli strain R3 is CGMCC (China General Microbiological Culture Collection Center) No.36025. The spathiphylli strain R3 has the advantages that the preservation number is CGMCC No.36025; the bacterial strain R3 has good bacteriostatic activity on phytophthora infestans, and meanwhile, the biocontrol agent formed by compounding the bacterial strain R3 with at least one of the bacillus safensis bacterial strain B1 and the leisei bacterial strains L1 and L3 or the combination of the bacterial strain R3 and the L6 has good prevention and control effects in pot experiments and field experiments.
Owner:CHINA AGRI UNIV

A gene REL that enhances plant disease resistance and its application

This invention provides a gene REL for enhancing plant disease resistance and its applications, belonging to the fields of plant molecular biology and plant genetic engineering. This invention relates to a plant-derived disease resistance gene REL, its recombinant expression vector, and its applications. The gene is derived from *Nicotiana benthamiana* and has the nucleotide sequence shown in SEQ ID NO.1, with the encoded product having the amino acid sequence shown in SEQ ID NO.3. This invention also provides a recombinant expression vector comprising the gene REL described herein. This gene plays a crucial role in tobacco resistance to various *Phytophthora* fungi. This invention can be applied to crop breeding for disease resistance improvement.
Owner:NANJING AGRICULTURAL UNIVERSITY

Plant immune-inducing protein EqBPIE1 and its application

ActiveCN120365387BBiocideAntibody mimetics/scaffoldsBiotechnologyColletotrichum gossypii
The present invention belongs to the field of molecular biology technology, and specifically relates to a plant immune-inducing protein EqBPIE1 and its application. The amino acid sequence of the protein EqBPIE1 is shown in SEQ ID No. 1. The protein EqBPIE1 provided by the present invention can enhance the plant's own immune system from the plant itself, thereby enabling the plant to cope with more diverse environmental changes and infection by multiple pathogens; it has an inhibitory effect on the infection of multiple pathogens on plants, covering multiple pathogens such as rubber tree powdery mildew, Siamese anthracnose, gray mold, Pseudomonas syringae, pepper phytophthora, and soybean phytophthora, and can significantly improve plant disease resistance. The antibacterial effect can be produced at a concentration of 0.1 to 1 ÎĽM.
Owner:HAINAN UNIVERSITY SANYA NANFAN RESEARCH INSTITUTE

Soybean seed coating agent as well as preparation method and application thereof

The invention relates to the technical field of seed treatment, in particular to a soybean seed coating agent as well as a preparation method and application thereof. Each 1kg of soybean seeds in the coating agent comprise the following components in parts by weight: 0.4-0.6 g of naringenin and 7-12g of auxiliary materials; the auxiliary material comprises one or more of a film-forming agent, an adhesive and a filling agent; the film-forming agent is polyvinyl alcohol, the adhesive is Arabic gum, and the filler is talcum powder. The invention further provides a preparation method of the soybean seed coating agent and application of the soybean seed coating agent in prevention and treatment of soybean root rot. The seed coating agent has the advantages that naringenin is used as a core active component, a uniform protective film is formed on the surface of a coated seed, the seed coating agent has double effects of bacteriostasis and crop protection, mycelial growth of main pathogenic bacteria such as soybean root rot such as phytophthora sojae and fusarium oxysporum can be remarkably inhibited, and the resistance of soybeans to pathogenic bacteria of different physiological races is improved. The coating agent is safe and environment-friendly, simple in preparation process and convenient to use, and meets the large-scale planting requirements of soybeans.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Application of rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var.

The invention provides application of rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus It is found for the first time that the rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Moreover, the strain has the capabilities of producing cellulase, fixing nitrogen, decomposing inorganic phosphorus and producing siderophores, and pot experiments show that the strain can promote the growth of peppers. The invention provides a new way for biological prevention and control of pepper phytophthora blight, and has positive significance for reducing the use of pesticides and improving the yield and quality of pepper.
Owner:YANGTZE UNIVERSITY

Aspergillus costaricensis GNX2461 and application of preparation of aspergillus costaricensis GNX2461

The invention discloses an Aspergillus costaricensis GNX2461 and an application of a preparation product of the Aspergillus costaricensis GNX2461. The Aspergillus costaricensis GNX2461 has the advantages that the Aspergillus costaricensis GNX2461 is Belongs to the technical field of biological control. According to the application, the prevention and treatment activity of the Aspergillus costaceticus on phytophthora is disclosed for the first time, a tobacco endophytic fungus Aspergillus costaceticus GNX2461 is successfully screened, and the Aspergillus costaceticus GNX2461 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on October 31, 2024, and the preservation number is CGMCC NO.41607. The tobacco endophytic fungus Aspergillus costaceticus GNX2461 has the advantages that the tobacco endophytic fungus Aspergillus costaceticus GNX2461 can be used for preventing and treating phytophthora; research finds that viable bacteria and secondary metabolites of the strain have good antibacterial activity on phytophthora, the antibacterial rate reaches 95%, pot experiment verifies that the strain can be planted in tobacco roots and has good prevention and control effects on tobacco black shank, and technical support is provided for biological prevention and control of tobacco black shank.
Owner:HEILONGJIANG TOBACCO IND +1

AS-PCR primer and method for detecting drug resistance of peronophythora litchii to azoxystrobin

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Use of reduced expression of a negative regulator gene in increasing resistance to phytophthora sojae in soybean

This invention discloses the application of reducing the expression of negatively regulated genes in improving resistance to Phytophthora sojae, belonging to the field of plant genetic engineering. Research has found that the soybean genes GmTIFY5a or GmTIFY5b have a negative regulatory function on resistance to Phytophthora sojae. Knocking out GmTIFY5a or GmTIFY5b, or a combination of GmTIFY5a and GmTIFY5b, can enhance soybean resistance to Phytophthora sojae. This can be applied to improving soybean resistance to diseases caused by Phytophthora sojae or to breeding soybean varieties resistant to these diseases. In the soybean cultivar "Williams 82," a double mutant lacking both GmTIFY5a and GmTIFY5b, the accumulation of Phytophthora sojae was reduced by 50-60% compared to the wild type, the lesion infection area was reduced by 80-90%, and the survival rate was increased by 20-30%. Knocking out GmTIFY5a and GmTIFY5b significantly improved plant resistance to Phytophthora sojae. By genetically engineering the above genes to combat plant root rot caused by septicemia, we can not only provide important genetic resources for plant molecular breeding, but also provide an effective means to achieve high plant yields.
Owner:SANYA INSTITUTE OF NANJING AGRICULTURAL UNIVERSITY

Spiropolyspora pink MLY32, microbial inoculum as well as preparation method and application of microbial inoculum

The invention discloses a strain of spiropolyspora pink MLY32, a microbial inoculum as well as a preparation method and application of the microbial inoculum. The MLY32 strain is obtained by multi-generation co-culture, domestication and breeding of a wild-type spiropolyspora roseus strain CR032 separated from rhizosphere soil of Guizhou Xinyi healthy tobacco plants and a wild-type phytophthora parasitica strain PpN03, can be attached to phytophthora parasitica hyphae through parasitism, and shows unique explosive growth, and the MLY32 strain can be applied to the field of tobacco phytophthora parasitica hyphae. After the MLY32 strain is parasitized, the proliferation speed of the parasitized tobacco phytophthora parasitica wild type strain PpN03 is greatly slowed down, so that the MLY32 strain has a strong bacteriolytic inhibition effect on the tobacco phytophthora parasitica, and the MLY32 strain is co-cultured with the non-pathogenic tobacco phytophthora parasitica MLY61 to obtain a fungicide spiropolyspora 3261 which still has strong parasitism and no pathogenic risk on the tobacco phytophthora parasitica wild type strain PpN03. The invention has wide application potential in the field of agricultural biological control fungicide.
Owner:CHINA TOBACCO HUNAN IND CORP

Phytophthora GLS1 protein as well as coding gene and application thereof

The invention discloses a glucosidase protein (GLS1) from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The PsGLS1 protein provided by the invention is a protein of which the sequence is as shown in SEQ ID No. 1 in Phytophthora sojae, and the PsGLS1 protein is a protein of which the sequence is as shown in SEQ ID No. 2 in Phytophthora sojae; the coding gene of the gene is shown as SEQ ID No.2. Experiments prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, and after the PsGLS1 gene is knocked out, the mycelial growth rate, the sporangium number, the zoospore yield and the resting spore germination rate of the phytophthora sojae are obviously reduced, and the pathogenicity of pathogenic bacteria is obviously influenced. Glucosidase inhibitor mispermine has an inhibition effect on the growth of phytophthora sojae hyphae. In conclusion, the PsGLS1 gene has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and a molecular target is provided for research and development of novel bactericides in the future.
Owner:CHINA AGRI UNIV

Pesticide composition containing chloromethylsilazane and application of pesticide composition in plant disease prevention and growth promotion

ActiveCN121100941ABiocidePlant growth regulatorsBrassicaCapsicum cardenasii
The invention discloses a pesticide composition containing chloromethylsilazane and application of the pesticide composition in plant disease prevention and growth promotion, and belongs to the technical field of biocide pesticides. According to the invention, the red date kernel extract and the digitaria sanguinalis extract are taken as synergistic extracts and are combined with the chloromethylsilatrane and the 14-hydroxy brassin to prepare the pesticide composition containing the chloromethylsilatrane. When the phytophthora capsici is applied to a test, in a pepper disease resistance test, the disease index is reduced to some extent, and the relative control effect is up to 79.45% at most, which indicates that the phytophthora capsici is inhibited; in the experiment of promoting the growth of the pepper, the plant height of the pepper plant in the compound group is 19.67 + / -0.23 cm, the stem diameter is 4.07 + / -0.05 mm, the dry weight of the overground part is 1.51 + / -0.11 g, the dry weight of the underground part is 0.39 + / -0.04 g, and the growth index is remarkably improved. The pesticide composition containing the chloromethylsilazane prepared by the invention can better inhibit phytophthora capsici, is suitable for developing pesticides for preventing and treating phytophthora capsici, and also has a better promoting effect on the growth of capsicum.
Owner:SHANDONG TONGFANG BIOTECHNOLOGY CO LTD

Gene GmABI5 for negative regulation of phytophthora sojae resistance and application of gene GmABI5

The invention discloses a GmABI5 gene for negative regulation and control of phytophthora sojae resistance and an application of the GmABI5 gene. By constructing a gene silencing expression vector of the GmABI5 gene, results of root scab length, phytophthora accumulation amount and the like of a soybean cultivar 'Williams 82' with low expression of the GmABI5 under the stress of phytophthora by utilizing an agrobacterium tumefaciens-mediated soybean instantaneous transformation system show that the resistance of a plant to the phytophthora can be remarkably improved by silencing the GmABI5. The gene is expected to be used in the following aspects: the disease resistance of phytophthora-sensitive soybeans to phytophthora root rot can be improved by editing the sequence by using a gene editing technology; and resistance gene resource mining and related mechanism research of soybean disease-resistant molecular breeding are facilitated.
Owner:NANJING AGRICULTURAL UNIVERSITY

A method for targeted removal of pathogenic fungi in continuous tobacco fields

The present application belongs to the technical field of tobacco planting, and particularly relates to a method for targeted removal of pathogenic bacteria in continuous tobacco field, which comprises the following steps: S1, land preparation: removing field residues and weeds before transplanting flue-cured tobacco seedlings; S2, pesticide application: applying base fertilizer and removal agent to the tobacco ridge area of the tobacco field; S3, ridging: loosening and ridging the soil where the removal agent is applied; S4, film mulching and sealing: mulching after ridging, and sealing the tobacco ridge; S5, film breaking and air release: breaking the film at the interval of the tobacco seedling nest distance and releasing the air in the film; S6, trial planting and verification: planting 3-5 tobacco seedlings or vegetable seedlings per mu, watering and observing, and then transplanting after no abnormality of the plants; S7, transplanting tobacco seedlings; in use, the inhibition rates of Fusarium, Pseudomonas and Pythium in the continuous tobacco field are all above 86%, the comprehensive removal rate of pathogenic bacteria is greater than or equal to 86%, the incidence of tobacco seedlings is reduced from 36.8% to less than 7%, the frequent occurrence of soil-borne diseases is solved from the source, the bacteria are targetedly inhibited, no fallow is needed, and the cost is low.
Owner:WUXI BRANCH CHONGQING CITY COMPANY OF CHINA NAT TOBACCO

Potato late blight biocontrol strain as well as biocontrol inoculant and application thereof

The invention provides a potato late blight biocontrol strain as well as a biocontrol inoculant and application thereof, and belongs to the technical field of biocontrol bacteria. The invention discloses a bacillus safeensis strain B1, and the biological preservation number of the bacillus safeensis strain B1 is CGMCC (China General Microbiological Culture Collection Center) No.36024. The invention also provides a biocontrol bacterium composition which is prepared by compounding the Leifsonia sp. Strain L1 alone or by combining the Leifsonia sp. Strain L1 with the Leifsonia sp. Strain L6 and the strain B1. The strain B1 has good activity of inhibiting phytophthora infestans, meanwhile, the biocontrol bacterium composition not only further improves the antibacterial activity of the strain B1, but also shows that the biocontrol bacterium composition can effectively reduce the disease index of potatoes, and compared with the single-phase strain B1, the biocontrol bacterium composition can effectively improve the prevention effect of potato late blight.
Owner:CHINA AGRI UNIV

Tobacco disease-resistant gene NbPRRK90 and application thereof in regulation and control of plant immunity

The invention discloses a tobacco disease-resistant gene NbPRRK90 and an application of the tobacco disease-resistant gene NbPRRK90 in regulation and control of plant immunity. Transcription mode analysis and plant expression analysis find that the NbPRRK90 can improve the disease resistance of plants to phytophthora, so that the tobacco disease-resistant gene NbPRRK90 can be applied to synthesis of new disease-resistant genes in plant disease-resistant breeding and the like, and is a disease-resistant material with high application value. The tobacco disease resistance related gene NbPRRK90 is transiently expressed in tobacco, phytophthora capsici is inoculated 2 days later, it can be observed that after NbPRRK90 expression, the number of disease spots is remarkably reduced compared with that of negative control, and biostatistical analysis shows that the areas of the two treated disease spots are remarkably different.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Pesticide composition containing silicon-rich ring and application thereof in plant disease prevention and growth promotion

The application discloses a pesticide composition containing silicon-rich rings and application thereof in plant disease prevention and growth promotion, and belongs to the technical field of biocidal pesticides. The application combines red jujube kernel extract and crabgrass extract as a synergistic extract with silicon-rich rings and 14-hydroxy brassinolide to prepare the pesticide composition containing silicon-rich rings. The pesticide composition is used in tests. In pepper disease resistance tests, the disease index is reduced, the highest relative prevention efficiency reaches 79.45%, and it is indicated that pepper phytophthora is inhibited. In the promotion experiment on pepper growth, the plant height of the pepper plants in the compound group is 19.67+ / -0.23 cm, the stem diameter is 4.07+ / -0.05 mm, the above-ground dry weight is 1.51+ / -0.11 g, and the underground dry weight is 0.39+ / -0.04 g, and the growth indexes are significantly improved. The pesticide composition containing silicon-rich rings prepared by the application can better inhibit pepper phytophthora, is suitable for the development of a pesticide for preventing and treating pepper phytophthora disease, and has a good promoting effect on the growth of the pepper.
Owner:SHANDONG TONGFANG BIOTECHNOLOGY CO LTD

Phytophthora gls1 protein, encoding gene and application thereof

ActiveCN121555474Breduce pathogenicityReduce stress damage marker protein contentMicrobiological testing/measurementMicroorganism based processesBiotechnologySporeling
This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.
Owner:CHINA AGRI UNIV

Effector factor NLP779 of Phytophthora capsici and its application

ActiveCN116789794BBiocideFungicidesBiotechnologyCallose
The present invention discloses the pepper phytophthora effector NLP779 and its application. The present invention reveals for the first time that NLP779 is a virulence factor of pepper phytophthora, and after spraying it on pepper plants, it can induce an immune response in pepper and inhibit the infection of pepper phytophthora. The stimulation of plant immunity was verified by ROS level detection, callose deposition, MAPKs phosphorylation, etc. Many hormones in plants, such as ethylene, jasmonic acid and salicylic acid, also regulate plant immunity. After exogenous application of NLP779, TMT proteomics analysis was performed, and compared with the control group, a large number of up-regulated proteins were enriched in the ethylene pathway, jasmonic acid pathway, and phenylpropanoid metabolic pathway, indicating that it regulates plant immunity through multiple pathways. The experimental results show that NLP779 has a dual identity as both a virulence factor and an elicitor. The present invention provides an effective means for the prevention and control of pepper phytophthora.
Owner:SHANDONG NORMAL UNIV

Gene gmsrc2 for improving resistance to phytophthora sojae and application thereof

The application discloses a GmSRC2 gene for improving resistance of soybean phytophthora and application, and the soybean plant with the GmSRC2 gene disclosed by the application enhances the resistance of the soybean to the soybean phytophthora, that is, the phenotype, disease spot area, phytophthora accumulation amount and hydrogen peroxide and other physiological indexes of the soybean cultivar "Jack" with overexpressed GmSRC2 under the stress of the phytophthora are detected, and the results show that the GmSRC2 plays an important positive regulation role in improving the resistance of the plant to the phytophthora. Through the gene engineering improvement of the plant resistance to the phytophthora root rot of the transformant, it is found that the gene has a certain effect on cultivating the plant variety with high resistance to the phytophthora root rot, and can improve the resistance of the plant to the phytophthora, thereby serving the improvement of the yield and quality of the plant.
Owner:NANJING AGRICULTURAL UNIVERSITY