42 results about "Phytophthora sp." patented technology

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention relates to a bactericidal composition containing fluxapyroxad and thiediazole copper. The bacterial composition is compounded from fluxapyroxad and thiediazole copper in a binary mode, wherein a mass ratio of fluxapyroxad to thiediazole copper is (1-50) to (50-1); a mass fraction of fluxapyroxad and thiediazole in the preparation is 1%-80%, and the rest of the preparation is auxiliary components which are allowed to use and are acceptable in a pesticide. A dosage form of the bactericidal composition is missible oil, a suspending agent, wettable powder, a water dispersible granule, emulsion in water, microemulsion, a granule and a micro-capsule. The bactericidal composition is mainly used for controlling plant gray mold, peronospora and phytophthora germs.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The application belongs to the field of enzyme genetic engineering, and particularly relates to application of a sesquiterpene synthase and a mutant thereof in synthesis of enantiomeric-drimenol compounds, wherein the sesquiterpene synthase is a sesquiterpene synthase from Chenopodium quinoa Willd., and includes CqDMDS1 or CqDMDS2; the mutant of the sesquiterpene synthase is a mutant CqDMDS1 in which an amino acid at position 443 of CqDMDS1 is mutated F443A / G The application realizes heterologous expression of the sesquiterpene synthase and the mutant thereof from Chenopodium quinoa Willd. in an Escherichia coli and a N. benthamiana expression system, catalyzes synthesis of (-)‑8,11-driman-diol or (+)‑drimenol. It is found through activity test that the sesquiterpene synthase from Chenopodium quinoa Willd. has a significant inhibitory effect on plant pathogenic fungi Phytophthora nicotianae, Phytophthora sojae and Fusarium graminearum. The sesquiterpene synthase and the mutant thereof and the biological preparation of the catalytic product have important significance for development of biological pesticides.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention provides a spathiphyllum conifer strain R3, a complex microbial inoculant thereof and application of the spathiphyllum conifer strain R3 in prevention and control of late blight, and belongs to the technical field of biocontrol bacteria. The invention relates to a spathiphylli strain R3, and the preservation number of the spathiphylli strain R3 is CGMCC (China General Microbiological Culture Collection Center) No.36025. The spathiphylli strain R3 has the advantages that the preservation number is CGMCC No.36025; the bacterial strain R3 has good bacteriostatic activity on phytophthora infestans, and meanwhile, the biocontrol agent formed by compounding the bacterial strain R3 with at least one of the bacillus safensis bacterial strain B1 and the leisei bacterial strains L1 and L3 or the combination of the bacterial strain R3 and the L6 has good prevention and control effects in pot experiments and field experiments.

The invention relates to the technical field of seed treatment, in particular to a soybean seed coating agent as well as a preparation method and application thereof. Each 1kg of soybean seeds in the coating agent comprise the following components in parts by weight: 0.4-0.6 g of naringenin and 7-12g of auxiliary materials; the auxiliary material comprises one or more of a film-forming agent, an adhesive and a filling agent; the film-forming agent is polyvinyl alcohol, the adhesive is Arabic gum, and the filler is talcum powder. The invention further provides a preparation method of the soybean seed coating agent and application of the soybean seed coating agent in prevention and treatment of soybean root rot. The seed coating agent has the advantages that naringenin is used as a core active component, a uniform protective film is formed on the surface of a coated seed, the seed coating agent has double effects of bacteriostasis and crop protection, mycelial growth of main pathogenic bacteria such as soybean root rot such as phytophthora sojae and fusarium oxysporum can be remarkably inhibited, and the resistance of soybeans to pathogenic bacteria of different physiological races is improved. The coating agent is safe and environment-friendly, simple in preparation process and convenient to use, and meets the large-scale planting requirements of soybeans.

The invention discloses AS-PCR primers and a method for detecting drug resistance of peronophythora litchii to azoxystrobin. An AS-PCR primer capable of being used for detecting whether peronophythora litchii has drug resistance to azoxystrobin or not is obtained by comparing differential sequences of a peronophythora litchii strain capable of resisting azoxystrobin and a peronophythora litchii strain sensitive to azoxystrobin, and a method for detecting the drug resistance of peronophythora litchii to azoxystrobin is constructed based on the AS-PCR primer. The detection specificity is good, and the sensitivity is high. The AS-PCR primer and the detection method are used for amplifying a to-be-detected strain, and if a single band with the consistent size can be obtained, the detected strain is anti-azoxystrobin peronophythora litchii. Compared with a traditional detection method, the detection method disclosed by the invention is short in time consumption, simple, convenient and feasible, is beneficial to quickly monitoring early epidemic of peronophythora litchii anti-azoxystrobin groups, and provides scientific guidance for scientific prevention and control of peronophythora litchii.

This invention discloses the application of reducing the expression of negatively regulated genes in improving resistance to Phytophthora sojae, belonging to the field of plant genetic engineering. Research has found that the soybean genes GmTIFY5a or GmTIFY5b have a negative regulatory function on resistance to Phytophthora sojae. Knocking out GmTIFY5a or GmTIFY5b, or a combination of GmTIFY5a and GmTIFY5b, can enhance soybean resistance to Phytophthora sojae. This can be applied to improving soybean resistance to diseases caused by Phytophthora sojae or to breeding soybean varieties resistant to these diseases. In the soybean cultivar "Williams 82," a double mutant lacking both GmTIFY5a and GmTIFY5b, the accumulation of Phytophthora sojae was reduced by 50-60% compared to the wild type, the lesion infection area was reduced by 80-90%, and the survival rate was increased by 20-30%. Knocking out GmTIFY5a and GmTIFY5b significantly improved plant resistance to Phytophthora sojae. By genetically engineering the above genes to combat plant root rot caused by septicemia, we can not only provide important genetic resources for plant molecular breeding, but also provide an effective means to achieve high plant yields.

The invention discloses a strain of spiropolyspora pink MLY32, a microbial inoculum as well as a preparation method and application of the microbial inoculum. The MLY32 strain is obtained by multi-generation co-culture, domestication and breeding of a wild-type spiropolyspora roseus strain CR032 separated from rhizosphere soil of Guizhou Xinyi healthy tobacco plants and a wild-type phytophthora parasitica strain PpN03, can be attached to phytophthora parasitica hyphae through parasitism, and shows unique explosive growth, and the MLY32 strain can be applied to the field of tobacco phytophthora parasitica hyphae. After the MLY32 strain is parasitized, the proliferation speed of the parasitized tobacco phytophthora parasitica wild type strain PpN03 is greatly slowed down, so that the MLY32 strain has a strong bacteriolytic inhibition effect on the tobacco phytophthora parasitica, and the MLY32 strain is co-cultured with the non-pathogenic tobacco phytophthora parasitica MLY61 to obtain a fungicide spiropolyspora 3261 which still has strong parasitism and no pathogenic risk on the tobacco phytophthora parasitica wild type strain PpN03. The invention has wide application potential in the field of agricultural biological control fungicide.

The invention discloses a glucosidase protein (GLS1) from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The PsGLS1 protein provided by the invention is a protein of which the sequence is as shown in SEQ ID No. 1 in Phytophthora sojae, and the PsGLS1 protein is a protein of which the sequence is as shown in SEQ ID No. 2 in Phytophthora sojae; the coding gene of the gene is shown as SEQ ID No.2. Experiments prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, and after the PsGLS1 gene is knocked out, the mycelial growth rate, the sporangium number, the zoospore yield and the resting spore germination rate of the phytophthora sojae are obviously reduced, and the pathogenicity of pathogenic bacteria is obviously influenced. Glucosidase inhibitor mispermine has an inhibition effect on the growth of phytophthora sojae hyphae. In conclusion, the PsGLS1 gene has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and a molecular target is provided for research and development of novel bactericides in the future.

The invention discloses a pesticide composition containing chloromethylsilazane and application of the pesticide composition in plant disease prevention and growth promotion, and belongs to the technical field of biocide pesticides. According to the invention, the red date kernel extract and the digitaria sanguinalis extract are taken as synergistic extracts and are combined with the chloromethylsilatrane and the 14-hydroxy brassin to prepare the pesticide composition containing the chloromethylsilatrane. When the phytophthora capsici is applied to a test, in a pepper disease resistance test, the disease index is reduced to some extent, and the relative control effect is up to 79.45% at most, which indicates that the phytophthora capsici is inhibited; in the experiment of promoting the growth of the pepper, the plant height of the pepper plant in the compound group is 19.67 + / -0.23 cm, the stem diameter is 4.07 + / -0.05 mm, the dry weight of the overground part is 1.51 + / -0.11 g, the dry weight of the underground part is 0.39 + / -0.04 g, and the growth index is remarkably improved. The pesticide composition containing the chloromethylsilazane prepared by the invention can better inhibit phytophthora capsici, is suitable for developing pesticides for preventing and treating phytophthora capsici, and also has a better promoting effect on the growth of capsicum.

The present application belongs to the technical field of tobacco planting, and particularly relates to a method for targeted removal of pathogenic bacteria in continuous tobacco field, which comprises the following steps: S1, land preparation: removing field residues and weeds before transplanting flue-cured tobacco seedlings; S2, pesticide application: applying base fertilizer and removal agent to the tobacco ridge area of the tobacco field; S3, ridging: loosening and ridging the soil where the removal agent is applied; S4, film mulching and sealing: mulching after ridging, and sealing the tobacco ridge; S5, film breaking and air release: breaking the film at the interval of the tobacco seedling nest distance and releasing the air in the film; S6, trial planting and verification: planting 3-5 tobacco seedlings or vegetable seedlings per mu, watering and observing, and then transplanting after no abnormality of the plants; S7, transplanting tobacco seedlings; in use, the inhibition rates of Fusarium, Pseudomonas and Pythium in the continuous tobacco field are all above 86%, the comprehensive removal rate of pathogenic bacteria is greater than or equal to 86%, the incidence of tobacco seedlings is reduced from 36.8% to less than 7%, the frequent occurrence of soil-borne diseases is solved from the source, the bacteria are targetedly inhibited, no fallow is needed, and the cost is low.

The invention provides a potato late blight biocontrol strain as well as a biocontrol inoculant and application thereof, and belongs to the technical field of biocontrol bacteria. The invention discloses a bacillus safeensis strain B1, and the biological preservation number of the bacillus safeensis strain B1 is CGMCC (China General Microbiological Culture Collection Center) No.36024. The invention also provides a biocontrol bacterium composition which is prepared by compounding the Leifsonia sp. Strain L1 alone or by combining the Leifsonia sp. Strain L1 with the Leifsonia sp. Strain L6 and the strain B1. The strain B1 has good activity of inhibiting phytophthora infestans, meanwhile, the biocontrol bacterium composition not only further improves the antibacterial activity of the strain B1, but also shows that the biocontrol bacterium composition can effectively reduce the disease index of potatoes, and compared with the single-phase strain B1, the biocontrol bacterium composition can effectively improve the prevention effect of potato late blight.

The application discloses a pesticide composition containing silicon-rich rings and application thereof in plant disease prevention and growth promotion, and belongs to the technical field of biocidal pesticides. The application combines red jujube kernel extract and crabgrass extract as a synergistic extract with silicon-rich rings and 14-hydroxy brassinolide to prepare the pesticide composition containing silicon-rich rings. The pesticide composition is used in tests. In pepper disease resistance tests, the disease index is reduced, the highest relative prevention efficiency reaches 79.45%, and it is indicated that pepper phytophthora is inhibited. In the promotion experiment on pepper growth, the plant height of the pepper plants in the compound group is 19.67+ / -0.23 cm, the stem diameter is 4.07+ / -0.05 mm, the above-ground dry weight is 1.51+ / -0.11 g, and the underground dry weight is 0.39+ / -0.04 g, and the growth indexes are significantly improved. The pesticide composition containing silicon-rich rings prepared by the application can better inhibit pepper phytophthora, is suitable for the development of a pesticide for preventing and treating pepper phytophthora disease, and has a good promoting effect on the growth of the pepper.

This invention discloses a glucosidase protein (GLS1) from *Phytophthora*, a plant pathogenic oomycete, its encoding gene, and its applications. The PsGLS1 protein provided by this invention is effective against *Phytophthora soybean* (…). Phytophthora sojae The protein in this invention is shown in SEQ ID No. 1; its encoding gene is shown in SEQ ID No. 2. Experiments have demonstrated that the protein provided by this invention plays an important role in the growth and development of *Phytophthora soybeani*. PsGLS1 Gene knockout significantly reduced the mycelial growth rate, sporangium number, zoospore yield, and resting spore germination rate of *Phytophthora soybeani*, and also significantly affected the pathogenicity of the fungus. The glucosidase inhibitor sericite also inhibited the mycelial growth of *Phytophthora soybeani*. In summary, this invention… PsGLS1 Genes have high application value in controlling the occurrence and spread of diseases caused by Phytophthora, and provide molecular targets for the development of new fungicides in the future.

This invention relates to the field of genetic engineering technology, and particularly to soybean GmST3 protein mutants and their application in regulating soybean resistance to Phytophthora infestans. This invention reveals for the first time that the soybean GmST3 protein and its encoding gene have the function of regulating soybean resistance to Phytophthora infestans. By reducing the expression of wild-type soybean GmST3 protein or its encoding gene, or by inducing soybean plants to express soybean GmST3 protein mutants, soybean resistance to Phytophthora infestans can be effectively improved. The soybean GmST3 protein mutants and their encoding genes provided by this invention can also be used for the breeding of soybean Phytophthora-resistant varieties, and have broad application prospects.

The application belongs to the technical field of plant disease resistance breeding, and provides application of GmbZIP111 protein and / or its coding gene in improving resistance of soybean to pythium. GmbZIP111 The gene positively regulates resistance of soybean to pythium root rot, overexpression of the gene can significantly inhibit pythium infection of soybean, and knockout of the gene leads to reduced resistance. GmbZIP111 The gene has important theoretical significance and application value for analyzing the mechanism of soybean resistance to pythium root rot and cultivating new disease-resistant varieties.

The invention discloses a chimeric immune receptor and application of the chimeric immune receptor to improvement of phytophthora resistance of plants, and belongs to the technical field of plant disease control. The chimeric immune receptor disclosed by the invention can specifically recognize the phytophthora sojae effector Avh85 or a homolog thereof, and can be activated only when a specific pathogenic bacterium effector exists, so that accurate recognition of target pathogenic bacteria is realized, and unnecessary energy consumption of a plant immune system is avoided. Once the chimeric immune receptor disclosed by the invention is activated, multiple and potent immunoreactions including significant up-regulation of defensive genes, active oxygen outbreak and MAPK phosphorylation can be initiated, a firm defensive barrier is formed together, and expansion of pathogenic bacteria can be rapidly and efficiently suppressed.

The invention belongs to the technical field of plant molecular biology, and discloses a soybean disease resistance-related protease GmRD21A gene, a coding protein thereof, and an application of the gene in plant disease resistance. The nucleotide sequence of the protease GmRD21A gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the polypeptide coded by the protease GmRD21A gene is as shown in SEQ ID NO. 2. Protein spectrum analysis of phytophthora sojae pectate lyase PsPL1 proves that the protease GmRD21A degrades pectate lyase secreted by phytophthora and can resist attack of pathogenic bacteria pectate lyase on plant cell walls, and meanwhile, overexpression of the GmRD21A gene in soybeans can remarkably improve the resistance of plants to phytophthora. The invention can be applied to crop breeding anti-epidemic disease improvement, and is hopeful to improve the disease resistance of plants to epidemic diseases, thereby achieving the purposes of increasing yield and reducing pesticide consumption.

The invention provides a primer probe composition, a kit and a method for detecting phytophthora vigna based on an enzyme-mediated double-index amplification technology. The invention specifically discloses a primer probe composition and a kit for detecting phytophthora vigna based on the enzyme-mediated double-index amplification technology, wherein the primer probe composition comprises an upstream primer F2 as shown in SEQ ID NO.2, a downstream primer R2 as shown in SEQ ID NO.8 and a probe RNA1 as shown in SEQ ID NO.13. The primer probe composition and the kit for detecting the phytophthora vigna are obtained by screening specific sequences in genomic sequences of the phytophthora vigna on the basis of an enzyme-mediated double-index amplification technology, the phytophthora vigna can be detected simply, rapidly, specifically and highly sensitively, and the lowest detectable concentration is 0.001 ng / mu l; key technical support is provided for early discovery, accurate diagnosis and targeted prevention and control of phytophthora vigna, and green and healthy development of the cowpea industry is powerfully promoted.

The invention belongs to the technical field of microorganisms, and particularly discloses bacillus velezensis MT1406 antagonizing tomato fusarium oxysporum and application of the bacillus velezensis MT1406 antagonizing tomato fusarium oxysporum. The bacillus velezensis MT1406 is classified and identified as bacillus velezensis, the preservation number is CGMCC No.34883, and the bacillus velezensis MT1406 is separated from rhizosphere soil of Motuo tomatoes in Tibet. The bacterial strain MT1406 has a bacteriostasis rate of 65.84% on tomato fusarium oxysporum (fusarium oxysporum), and has broad-spectrum antagonistic activity on five fungi such as botrytis cinerea and phytophthora capsici and one oomycete (phytophthora capsici); meanwhile, the strain has potassium dissolving capacity (capable of converting insoluble potassium in soil) and protease producing capacity (D / d value is 3.76). Pot experiments show that the control effect of the strain on tomato fusarium wilt reaches 67.78%, and the control effect is obviously better than that of 30% hymexazol and other microbial agents sold in the market. The strain MT1406 disclosed by the invention has the functions of inhibiting bacteria and promoting growth, is green and safe, can be used for preparing a tomato fusarium wilt biocontrol agent, and provides an efficient strain resource for green prevention and control of tomato fusarium wilt.

The invention discloses a phytophthora sojae inducible promoter GmJAZ6 and application thereof, and belongs to the technical field of gene engineering. In order to meet the requirements of crop molecular breeding on resistance and growth balance, the invention provides a phytophthora sojae inducible promoter GmJAZ6, and the nucleotide sequence of the inducible promoter GmJAZ6 is as shown in SEQ ID NO. 1; the inducible promoter GmJAZ6 can obviously promote the expression of a reporter gene GUS under the condition that the inducible promoter GmJAZ6 is infected, so that the expression of a disease-resistant gene is driven, the resistance of the inducible promoter GmJAZ6 to the soybean phytophthora root rot is enhanced, and a new way is provided for the disease-resistant breeding of plants.

The invention discloses a DHE fluorescence intensity-based high-flux targeted phytophthora elicitin antibacterial compound screening method and application, and belongs to the field of biotechnology and agricultural disease control. According to the method, a to-be-detected compound, an elicitin protein and fluorescent sterol DHE are jointly incubated in a PBS buffer system, the fluorescence value after the DHE and the elicitin are combined is used as a contrast, and the fluorescence intensity is reduced after the to-be-detected compound is competitively combined with the elicitin, and the fluorescence intensity of the to-be-detected compound is detected by detecting the change of the fluorescence intensity of the reaction system. And rapid and high-throughput screening of the active compound capable of specifically targeting the elicitin-sterol binding site is realized. The screened compound has a wide antibacterial spectrum and good inhibitory activity on various important plant pathogenic fungi, and shows huge potential as a broad-spectrum botanical fungicide lead compound.

The invention discloses a small-molecule inhibitor of a targeted phytophthora sterol sensing receptor SSRK1 and application of the small-molecule inhibitor, and belongs to the technical field of pesticide biology. The inhibitor can specifically bind to a sterol binding site of SSRK1 and competitively block a natural sterol signal, so that comprehensive inhibition on growth, development and pathogenicity of phytophthora is realized. The compound shows remarkable bacteriostatic activity on various phytophthora (such as phytophthora sojae, phytophthora infestans and phytophthora capsici), and can effectively prevent and treat crop diseases such as soybean root rot, potato late blight and phytophthora capsici. The small-molecule inhibitor provided by the invention has the characteristics of novel target spot, unique action mechanism, broad spectrum, high efficiency, environmental friendliness and the like, is not easy to generate cross resistance with the traditional bactericide, and provides a new candidate molecule and preparation development approach for green prevention and control of phytophthora.

The present application relates to the application of spermine and / or spermidine in preventing and treating plant diseases caused by Phytophthora. The present application finds that spermine and spermidine can effectively inhibit the spore germination and mycelium growth of Phytophthora capsici Leonian and P. infestans Mont. and can effectively prevent and treat the plant diseases caused by P. capsici Leonian and P. infestans Mont., including pepper Phytophthora blight, tomato Phytophthora blight, potato late blight and tomato late blight, and significantly reduce the plant disease area.

The present invention relates to a method for controlling or preventing an infestation of useful plants by a phytopathogenic microorganism selected from the group consisting of Fusarium pseudograminearum, Fusarium culmorum, Fusarium oxysporum, Fusarium graminearum, Bakania oryzae, Sporidesmium humicola, Botrytis cinerea, Phytophthora sojae, Phytophthora capsici, Phytophthora infestans, Pythium aphanidermatum, Pythium arrhenomanes, Pythium deliense, Puccinia striiformis, Puccinia hordei, Puccinia malvaccarum, Pseudoperonospora cubensis and Sphaerotheca fuliginea, which comprises the application of a compound of formula (I) to useful plants, to the locus thereof, or to their propagation material.

The application discloses a Trichoderma koningiopsis strain and application thereof, and belongs to the technical field of microorganisms. Trichoderma koningiopsis The preservation number of the Trichoderma koningiopsis strain (M14) provided by the application is CCTCC NO: M 20261001. Trichoderma koningiopsis The Trichoderma koningiopsis strain (M14) provided by the application has good inhibiting effects on Fusarium oxysporum, Fusarium solani, Phytophthora nicotianae, Rhizopus arrhizus, Aphanomyces eurysipes, Rhizoctonia solani and Botryosphaeria dothidea, and has the abilities of promoting seed germination and plant growth, and therefore, the Trichoderma koningiopsis strain M14 has a good application prospect.

The invention provides a thiophene boric acid ester compound with a novel structure. The compound has a remarkable inhibition effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as pyricularia oryzae, colletotrichum, gibberellic disease bacteria, phytophthora and phytophthora capsici).