523 results about "Phytophthora sp." patented technology

Fungal / bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal / bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal / bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

Fungal / bacterial antagonist combinations, a seed coated with one of the combinations and a plant protected from plant pathogens by one of the combinations. The invention is also a fungal / bacterial antagonist combination comprising a Trichoderma virens fungal antagonist and a Bacillus subtilis var. amyloliquefaciens (Bacillus amyloliquefaciens) bacterial antagonist and its use for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. The invention also finds utility as a fungal / bacterial antagonist combination applied to the seed, stalk or leaf that results in an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

The invention discloses bacillus subtilis NB12, as well as a culture method and an application thereof. The strain with a preservation number of CGMCC No.5383 is preserved in China General Microbiological Culture Collection Center on October 24, 2011. Researches prove that: the strain has strong inhibition effects to rhizoctonia solani, colletotrichum musae, pyricularia grisea, fusarium graminearum, fusarium oxysporum f.sp.cubense, peronophythora litchi, ustilago scitaminea, fulvia fulva, xanthomonas oryzae and xanthomonas oryzae pv.oryzicola, is a broad-spectrum antagonistic strain, can be used for prevention and control of various plant diseases, has a particular good prevention and control effect to the rice sheath blight and damping off in seedling phase of cowpea, has the advantages of harmlessness to human and livestock, greenness and safety, good environment compatibility, difficult resistance generation and the like, can be used for commercial development, and has a wide application prospect.

The invention discloses bacillus amyloliquefaciens JK6 and biological fertilizer and application. The serial number of strain preservation is CGMCC No. 10658 and is stored in the China general microbiological culture collection center on March 23, 2015. The bacillus amyloliquefaciens JK6 is mainly used for biological prevention and control of pseudomonas solanacearum and is provided with an iron-production carrier, a biological membrane, protease, catalase, cellulose and IAA characteristics, multiple fungal pathogens of banana wilt pathogen, litchi anthrax germs, litchi frost phytophthora pathogens, rice blast fungus, cucumber wilt pathogen and the like, and growth of lettuce plants is obviously promoted; the K6 strain can be successfully planted, and disease preventing functional genes of yndj, srfAB, fend and ituC are expressed in soil; the strain is high in reproduction speed, simple in production technology, high in adverse resistance, easy to store and favorable for industrialized production, the biological fertilizer is prepared with the strain, soil-born diseases can be prevented and controlled, growth of the plants can be promoted, and application prospect is broad.

The invention discloses bacillus amyloliquefaciens and the microbial inoculum and application thereof. Bacillus amyloliquefaciens is a Latin name, the strain number is HC200, and the accession number in the China General Microbiological Culture Collection Center is CGMCC No.10371. The bacillus amyloliquefaciens has a selective antagonism effect on biocontrol bacteria and pathogenic bacteria and has a strong inhibiting effect on fusarium oxysporum, fusarium solani, phytophthora nicotianae and ralstonia solanacearum, and has a good synergetic antagonism effect on pathogenic bacteria by being matched with trichoderma viride H06.

The invention discloses burkholderia cepacia SD7 and a culturing method and application thereof. The bacterial strain is preserved in China General Microbiological Culture Collection Center on October 24th, 2001, and the preservation number is CGMCC No.5384. Through research, the bacterial strain has strong inhibiting effect on rhizoctonia solani, collectotrichum musae, pyricularia grisea, gibberella zeae, fusarium oxysporum f. sp. cubense, peronophythora litchi, ustiago scitamimea syd, fulvia fulva, xanthomonas oryzaepv.oryzae, xanthomonas oryzae pv. Oryzicola, and the like, is broad-spectrum antagonistic bacteria, can be used for preventing and treating a plurality of plant diseases, particularly has better preventing and treating effect for banana fruit anthracnose and rice sheath blight, has the advantages of harmlessness to person and animals, environmental protection and safety, good environmental compatibility, and difficulty in becoming resistant, is good for business development, and has broad application prospect.

The invention discloses a strain of efficient bacillus amyloliquefaciens, and a bacterial agent and application thereof, wherein the Latin name of the bacillus amyloliquefaciens is Bacillus amyloliquefaciens; the strain number is HW05; the accession number of the bacillus amyloliquefaciens in China General Microbiological Culture Collection Center is CGMCC No. 10273. The efficient bacillus amyloliquefaciens provided by the invention has the obvious inhibition effects on fusarium oxysporum, phytophthora capsici, ralstonia solanacearum, pyricularia grisea, colletotrichum gloeosporioides and root-knot nematode. Through the single application of HW05, the efficient prevention and control effects can be achieved on leaf surface disease sweet melon powdery mildew, mango anthracnose diseases andmulberry red rust diseases; the HW05 and paecilomyces lilacinus can be matched for preventing and treating soil-borne banana wilting diseases and banana root-knot nematode diseases, and a good synergyeffect is achieved.

The present invention relates to a bactericide composition containing dimethomorph and its preparation and application. The said composition is compounded composed of dimethomorph, cymoxanil and / or dimethormorph, cymoxanil, metalaxyl and / or d dimethormorph, phosethyl-Al and mancozeb. Its characteristic is the weight percentage of said effective components is: dimethormorph 1 to 90 percent; cymoxanil 1 to 90 percent; metalaxyl 1 to 90 percent; phosethyl-Al 1 to 90 percent; mancozeb 1 to 90 percent. The said composition can be prepared into missible oil, wettable powder, soluble powder, water emulsion, micro emulsion, water solution, suspending agent, microcapsule, or water dispersible granule. The said composition can be used for preventing and controlling plant diseases caused by peronospora, false peronospora and phytophthora. Indoor tests have shown that the said composition has obvious synergies, and good activity for postponing insecticide resistance of pathogenic bacteria.

The invention relates to a soilless seedling culturing substrate for resisting diseases and root nematode of plants and a production method thereof. In the invention, organic wastes such as straws are taken as raw materials, inoculated with zymogen according to certain proportion, subjected to moisture content regulation, evenly mixed and stacked for primary fermentation; when the temperature of a pile reaches 60 DEG C, the pile is turned; the pile is turned and oxygen and moisture content are supplemented every seven to ten days afterwards; after the pile is turned for three times, when the temperature is lower than 35 DEG C, the pile is inoculated with a bio-antagonist for secondary fermentation, turned every three to four days and kept at a temperature of between 35 and 40 DEG C; and after 15 to 20 days, the seedling culturing substrate containing the 10 bio-antagonist is obtained. The seedling culturing substrate has chemiotaxis for interfering pathogenicbacteria and nematode, can reduce infestation chance of the pathogenicbacteria and nematode on the root surface, and has double effects of resisting phytophthora disease and root nematode disease through the secondary fermentation and microorganism activation technology.

The invention discloses streptomyces katrae NB 20, as well as a culture method and an application thereof. The strain with a preservation number of CGMCC No.5382 is preserved in China General Microbiological Culture Collection Center on October 24, 2011. Researches prove that: the strain has strong inhibition effects to colletotrichum musae, pyricularia grisea, fusarium graminearum, fusarium oxysporum f.sp.cubense, peronophythora litchi, ustilago scitaminea, fulvia fulva, xanthomonas oryzae and xanthomonas oryzae pv.oryzicola, is a broad-spectrum antagonistic strain, can be used for prevention and control of various plant diseases, has a particular good prevention and control effect to the colletotrichum musae, has the advantages of harmlessness to human and livestock, greenness and safety, good environment compatibility, difficult resistance generation and the like, and has a wide application prospect.

The invention discloses bacillus velezensis and an application thereof to preventing and treating tomato phytophthora root rot. The invention provides bacillus velezensis (bacillus velezensis) ZF50, and the preservation number of the bacillus velezensis in the China General Microbiological Culture Collection Center is CMCC No. 16775. The invention provides an efficient biocontrol strain for preventing and treating the tomato phytophthora root rot, and bacillus velezensis has important production significance for a green prevention and control technique of tomato diseases and promotion of sustainable development of the tomato industry.

The invention belongs to the technical field of biological pesticides, discloses a Streptomyces l avendulae xjy strain which has been collected in the general microorganism center of CCCCM in 8.16.2007 with the collection number: CCMCC No.2130. The invention also discloses a separation and authentication method for the strain, a preparation method for antibiotic active substances of the Streptomyces l avendulae xjy strain, authentication for analysis structures of the active substances, prevention and cure experiments for metabolic active substances of the xjy strain against leaf muld of tomato, powdery mildew of cucumber and phytophthora blight of pepper, and growth-promoting experiments for the growth of the strain. The antibiotic active substances secreted by Streptomyces xjy strain is mainly nucleosides antibiotic having the characteristics of persistent effect and no medicinal hazard as well as having the effects of obviously preventing and curing vegetable diseases and promoting the growth to a certain extent.

The invention provides bacillus subtilis and preparation and application of a microbial inoculum thereof. The invention mainly relates to a Bacillus subtilis Y-1 of which the preservation number is CGMCC No.6539, and also relates to the microbial inoculum which is prepared from the bacillus subtilis, and application of the microbial inoculum. The Y-1 strain can be colonized on an apple tree, a polar tree, a cucumber and the rhizosphere of capsicums and in an apple tree, and has efficient and broad-spectrum antibacterial activity on fusarium, rhizoctonia solani, pestallozzia theae, phytophthora and the like; the microbial inoculum of the bacillus subtilis can be used for controlling diseases and continuous cropping obstacles of an apple tree, a polar tree, a cucumber and capsicums caused by those disease fungi. The preparation method of the microbial inoculum is simple in process, short in fermentation cycle, low in cost, and favorable for industrial production.

The invention discloses a pesticide composite containing pyraclostrobin. The pesticide composite contains active ingredients, namely pyraclostrobin and one of triazole compounds: flusilazole, epoxiconazole, tetraconazole, tricyclazole and propiconazole. The ratio of the parts of the two active ingredients by weight is 60 to 1-1 to 60. The pesticide composite at least contains one type of surfactant, thus being beneficial to the dispersion of the active ingredients in water. The pesticide composite integrates protection activity, treatment activity with eradication activity. The pesticide composite can be used for various crop plants such as wheat, paddy rice, vegetables, fruit trees and the like; and the pesticide composite can prevent and treat diseases such as downy mildew, phytophthora disease, rust disease, powdery mildew, alternaria mali roberts, leaf spot, scab disease, rice blast and the like.

The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to iron deficient growth conditions. The methods use molecular genetic markers to identify, select and / or construct disease-tolerant plants or identify and counterselect disease-susceptible plants. Soybean plants that display tolerance or improved tolerance to Phytophthora root rot infection that are generated by the methods of the invention are also a feature of the invention.

The invention relates to a sterilization composition containing cyanoacrylate bactericide and metalaxyl-M. The sterilization composition is prepared by carrying out binary compounding on effective components, namely the cyanoacrylate bactericide and the metalaxyl-M. The mass ratio of the cyanoacrylate bactericide to penconazole is 1-40 to 40-1; the mass percentage of the effective components, namely the cyanoacrylate bactericide and the metalaxyl-M, in the preparation is 1-80 percent; and other components are pesticidally allowable and acceptable auxiliary components. The sterilization composition can be in the form of missible oil, suspension agent, wettable powder, water diffusion particle, water emulsion, microemulsion, granules and micro capsules and can be used for preventing and treating downy mildew, phytophthora root rot, pythium, wheat gibberellic disease, cotton fusarium wilt, banana panama disease, fusarium moniliforme sheld, watermelon fusarium wilt and the like.

The present invention discloses a method for control of phytophthora blight of pepper by mixed application of trichoderma preparation and fungicides. The method comprises: seeding after mixing pepper seeds with the trichoderma preparation during seeding, or, applying the trichoderma preparation during pepper seedling transplanting and planting; during pepper cultivation, applying the fungicides before the occurrence of the disease or in early days of the disease; wherein the active strain in the trichoderma preparation is Trichodermaasperellum Thz01, and the preservation number thereof is CGMCC No. 6422; the fungicides are one or both of eugenol and mandipropamid. The trichoderma preparation used in the method of the invention has good control effect on phytophthora blight of pepper in the fields, and can effectively reduce the primary infection source when being used alone; while the additional application of the fungicides can effectively control disease epidemics. According to the method of the invention, the application amount of the fungicides is reduced, so residues of pesticides in the soil and pepper products are decreased, thereby ensuring environmental and product safety.

The invention discloses a Rhodopseudomonas strain. The name of the Rhodopseudomonas strain is Rhodopseudomonas PSB-06, and the collection number is CCTCC NO:M2012518. The invention also discloses a biocontrol microbial inoculum which is obtained through the activation, seed culture and production culture of the strain. The preparation of the biocontrol microbial inoculum comprises the multiple steps of activation, seed culture and production culture. The invention also discloses a Rhodopseudomonas strain biocontrol fermentation liquid which is prepared from the biocontrol microbial inoculum through fermentation and centrifugation. Each of the Rhodopseudomonas strain, the biocontrol microbial inoculum and the biocontrol fermentation liquid can be used in antagonizing phytophthora capsici or controlling phytophthora blight of pepper.

The invention provides bacillus amyloliquefaciens cd5bc. The strain is classified and named as Bacillus amyloliquefaciens cd5bc and is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO:M 2016232, and the preservation time is April 28, 2016. The invention further provides application of the strain in the aspect of preventing and controlling crop diseases and a biocontrol preparation prepared from the strain. The strain has a very significant preventing and controlling effect on red pepper phytophthora blight, the biocontrol effect reaches up to 100% after inoculation is conducted for 15 days, and the biocontrol effect reaches up to 82.36% after inoculation is conducted for 60 days. The bacillus amyloliquefaciens cd5bc has excellent disease-resistant effects on multiple crop diseases, the bacteriostasis rates of the strain on pathogenic bacteria of red pepper sclerotinia blight, bitter gourd wilt, cucumber wilt, cucumber scabs, rice blast, a rice sheath blight disease, broad bean chocolate spots and broad bean brown spots all reach up to 70% or above, and the bacteriostasis rate of the strain on the broad bean chocolate spots and broad bean brown spots reaches up to 100%.

The invention discloses a microbial inoculum for preventing and curing fungal diseases of plants; the active ingredients contain Trichoderma longibrachiatum and Trichoderma citrinoviride. The microbial inoculum of the invention is used to carry out control efficiency experiments and the results show that the control efficiency of liquid microbial inoculum to cucumber blight is up to 54.8% and thecontrol efficiency to green pepper phytophthora disease is up to 67.9%; the control efficiency of solid microbial inoculum to cucumber blight is up to 45.12% and the yield of cucumber is increased by11.06% so that the effect for preventing diseases and increasing yield is obvious; the control efficiency of solid microbial inoculum to green pepper phytophthora disease is up to 61.90% and the average plant height of the green pepper in experimental group is increased by 8.92% compared with that of the green pepper in control group and the yield of green pepper is increased by 37.19% so that the microbial inoculum of the invention is shown to have obvious effect for preventing increases, promoting the growth and increasing the yield.

The invention is an protein exciton, able to induce the disease resistance of plant system, separated from phytophthora and the preparing method. It uses the deposition of ammonium sulfate to obtain a coarse protein exciton, and then makes purification by ion-exchange chromatography and hydrophobe-interaction churomatography to obtain a 90kDa protein excition. The excition can be applied to induce system broad-spectrum disease resistance of tobacco and little Chinese cabbage. The use concentration is 0.1-10nM. The effect of prevention and treatment is above 80%.

The invention relates to the technical field of plant cultivation and specifically relates to a compound bacterium for preventing and treating tobacco phytophthora, a preparation method and an application thereof. The compound bacterium for preventing and treating tobacco phytophthora is mainly prepared from the following bacteria: streptomyces microflavus, photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate-releasing bacteria and potassium-releasing bacteria. Streptomyces microflavus is selected as antagonistic bacteria and photosynthetic bacteria, nitrobacteria, nitrogen-fixing bacteria, phosphate-releasing bacteria and potassium-releasing bacteria are selected as nutritional functional bacteria, so that the compound bacterium for preventing and treating tobacco phytophthora, provided by the invention, has a certain antagonistic effect to the pathogenic bacteria of the tobacco phytophthora and can effectively prevent the harm of the tobacco phytophthora. The compound bacterium for preventing and treating tobacco phytophthora, provided by the invention, can be directly used for planting the plants, inoculating composts and producing bio-organic fertilizer.

The invention relates to a bactericidal composite with fluopicolide and dimethomorph which is characterized in that the active components are fluopicolide and dimethomorph, the weight ratio of fluopicolide to dimethomorph is 1:30-10:1, the optimal weight ratio is 1:10-1:5, the total content of the active components is 0.5%-80% and the other components are auxiliary components. The recomposed pesticide of fluopicolide and dimethomorph can significantly promote the permeation and transmission of fluopicolide in plants, has obvious synergy, and can control the oomycete diseases of various crops effectively and delay the generation of resistibility. The bactericidal composite with fluopicolide and dimethomorph can be used for the disease control of crops such as fruit trees, vegetables and thelike, in particular to the diseases such as cucumber downy mildew, grape downy mildew, tomato downy mildew, tomato late blight, pepper phytophthora blight and the like.

The invention discloses a sterilization composition containing isopyrazam and carbendazim and application of the sterilization composition. The sterilization composition comprises isopyrazam and carbendazim as main effective components, wherein the mass ratio of isopyrazam to carbendazim is (1-70):(1-50). The sterilization composition can be used for preventing and treating diseases of cereals, fruit trees and vegetables, has a relatively good synergistic effect, overcomes and retards drug resistance of bacteria and is high in sterilization speed and long in lasting period, the application cost is lowered, and the prevention and treatment effect of the sterilization composition is remarkably superior to that of a single reagent. The sterilization composition can be used for preventing and treating fungous diseases of crops, particularly fungous diseases such as rice sheath blight diseases, rice blast and pepper phytophthora blight, and the effect of the sterilization composition is remarkably superior to that of the single reagent.

The invention discloses a Phytophthora nicotianae molecule detection primer and a detection method thereof, specially used for the specific molecule detection of Phytophthora nicotianae. A pair of specific primers of the Phytophthora nicotianae (comprising an upstream primer YhF:5'-GACATGATATCAACTGTTCTGCAG-3' and a downstream primer YhR:5'-CCTTGGATCTTCTCTCGATAAG-3') are mainly designed, and an amplified product having a fragment length of 342bp can be specially amplified on the pure DNA and bacteria bearing pathogenic tissues of the Phytophthora nicotianae through PCR amplification and agarose gel electrophoresis. The primer and the method can be used for the rapid, sensitive and specific detection of the Phytophthora nicotianae in Phytophthora nicotianae infected plant tissues in the production practices, can be used for the early stage diagnosis of field diseases and the monitoring and identification of pathogens, and provide reliable technologic and theoretic bases for the control of diseases caused by the Phytophthora nicotianae.

The present invention is in the field of soybean genetics. More specifically, the invention relates to nucleic acid molecules from regions the soybean genome, which are associated with soybean Phytophthora resistance. The invention also relates to proteins encoded by such nucleic acid molecules as well as antibodies capable of recognizing these proteins. The invention also relates to nucleic acid markers from regions the soybean genome, which are associated with soybean Phytophthora resistance. Moreover, the invention relates to uses of such molecules, including, transforming soybean Phytophthora resistant soybean with constructs containing nucleic acid molecules from regions the soybean genome, which are associated with such resistance. Furthermore, the invention relates to the use of such molecules in a plant breeding program and genetic manipulation of soybean for enhanced disease resistance.

The invention discloses an oleanolic acid oxime ester derivate compound, a preparation method and an application thereof. The structural formula of the compound is shown in formula I, and in the formula I, R refers to furyl, pyridyl, 1-menaphthyl, 4-chlorophenoxy methyl or phenyl with 1 to 2 substitutional groups; and the substitutional groups in phenyl with 1 to 2 substitutional groups are halogen (such as fluorine, chlorine, bromine and iodine) or nitryl. The preparation method comprises the following steps: 1) performing the esterification reaction between oleanolic oxime subject to benzylation protection and substituted carboxylic acids to prepare an oxime ester compound; and 2) removing benzylation protection on the oxime ester compound by Pd / C-H2 to prepare the compound in the formula I. The bactericidal activity test result shows that CAU2012-A has a favorable effect of inhibiting growth of sclerotinia scleotiorum, phytophthora melongenae, botrytis cintrea, pyricularia grisea, rhizoctonia solani and cotton fusarium wilt.

The invention relates to a molecule detection technique of cayenne pepper phytophthora capsici pectin lyase (Pcpel) 1 genes, belonging to the field of molecular biology. A pair of specific primers aredesigned and synthesized by the cayenne (sweet) pepper phytophthora capsici pectin lyase (Pcpel) 1 genes, the specificity of a plurality of tested bacteria species is verified by PCR, and then the sensitivity of the specific primers is verified by carrying out quantitative molecule detection for a soil cayenne pepper phytophthora oospore and a zoospore; and on the basis, the qualitative and quantitative molecule detection and the early warning of cayenne pepper phytophthora capsici contained in cayenne pepper diseased field soil, diseased stems, diseased leaves and diseased fruits are carriedout. The invention can detect and early warn the cayenne pepper phytophthora capsici in different periods and pathopoiesia characters thereof, thereby being convenient to establish a comprehensive prevention and control technology strategy, determining and selecting an optimal prevention and control period to carry out comprehensive prevention and control and high-efficiency management for the cayenne pepper phytophthora capsici, really controlling the infection and the harm of pathogenic bacteria from a source and enhancing the comprehensive prevention and control effect of the cayenne (sweet) pepper phytophthora capsici.

The invention discloses streptomyces antibioticu and biological preparation prepared from an active product thereof and application. The streptomyces antibioticu is a strain which is screened from more than 2,000 marine actinomycetes and has good inhibiting activity on phytophthora capsici, phytophthora cucumisativus, colletorichum cucumisativus and colletotrichum capsici; and the name of the strain is streptomyces antibioticu H74-18, the strain was collected in the China Center for Type Culture Collection on December 27th, 2010, and the collection number is CCTCC NO: M2010364. Antimycin A18(1) with bacteriostatic activity is separated from the strain, the molecular formula of the antimycin A18(1) is C28H41N2O9, and the molecular weight of the antimycin A18(1) is 549.3. The antimycin A18(1), an emulsifier and methanol are mixed to form the biological preparation with good stability. In 12 days after the biological preparation is sprayed, under the application times of 500x, the effect of preventing and controlling the phytophthora capsici is 90.00 percent, the effect of preventing and controlling the phytophthora cucumisativus is 86.67 percent, the effect of preventing and controlling the colletotrichum capsici is 86.67 percent and the effect of preventing and controlling the colletorichum cucumisativus is 90.00 percent.