111 results about "Phytophthora sp." patented technology

The invention discloses a phytophthora plant pathogenic oomycete histone lysine methyl transfer protein as well as a coding gene and application thereof. Wherein the phytophthora sojae histone lysine methyltransferase KMT3-1 is as shown in a sequence 2 in the specification, and the phytophthora sojae histone lysine methyltransferase KMT3-1 is as shown in a sequence 2 in the specification; the coding gene is shown as a sequence 1. Experiments prove that the KMT3-1 protein plays an important role in the growth and development process of phytophthora sojae, specifically, after the protein is deleted, the growth rate of phytophthora hyphae is slowed down, the sporangium yield is remarkably reduced, the release of zoospores is stopped, the oospore yield is reduced, the pathogenicity is reduced, and the like. Corresponding histone methyltransferase inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The gene provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed on the basis of the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The present invention discloses a gene, GmCDK8, that negatively regulates soybean resistance to Phytophthora spp., and its applications. The disclosed GmCDK8 knockout soybean plants exhibit enhanced resistance to the fungus. Specifically, the results of the soybean cultivar "Williams 82," which lacks GmCDK8, under Phytophthora stress, including root lesion length, leaf lesion area, Phytophthora accumulation, and infected necrotic area on leaves, indicate that knocking out GmCDK8 significantly improves plant resistance to Phytophthora spp. Genetic engineering of this gene for plant resistance to Phytophthora root rot not only provides an important genetic resource for plant molecular breeding but also offers an effective means for achieving high plant yields.

The invention discloses a gene GmCDK8 for negative regulation and control of phytophthora sojae resistance and application of the gene GmCDK8. GmCDK8-knocked-out soybean plants disclosed by the invention enhance the resistance of soybeans to phytophthora sojae; the results of root scab length, leaf scab area, phytophthora accumulation amount, leaf infected and necrotic areas and the like of the soybean cultivar Williams 82 without the GmCDK8 under the stress of phytophthora show that the resistance of the plant to the phytophthora can be remarkably improved by knocking out the GmCDK8. Genetic engineering improvement of plant phytophthora root rot resistance is carried out through the gene, important gene resources are provided for plant molecular breeding, and an effective means is provided for high yield of plants.

The invention relates to a bactericidal composition containing fluxapyroxad and thiediazole copper. The bacterial composition is compounded from fluxapyroxad and thiediazole copper in a binary mode, wherein a mass ratio of fluxapyroxad to thiediazole copper is (1-50) to (50-1); a mass fraction of fluxapyroxad and thiediazole in the preparation is 1%-80%, and the rest of the preparation is auxiliary components which are allowed to use and are acceptable in a pesticide. A dosage form of the bactericidal composition is missible oil, a suspending agent, wettable powder, a water dispersible granule, emulsion in water, microemulsion, a granule and a micro-capsule. The bactericidal composition is mainly used for controlling plant gray mold, peronospora and phytophthora germs.

The invention discloses an ADF protein from phytophthora as well as a coding gene and application thereof. According to the present invention, the PsADF protein provided by the present invention is the protein represented by sequences 2 and 5 in Phytophthora sojae (Phytophthora sojae); the coding gene is shown as a sequence 3 and a sequence 4. Tests prove that the protein provided by the invention plays an important role in the growth and development process of phytophthora sojae, specifically, after the 64-site mutation of the key amino acid functional site of the protein, the sporangium abnormality and zoospore quantity of the phytophthora sojae are reduced, and the pathogenicity is reduced. The protein provided by the invention has very high application value in control of epidemic disease occurrence and epidemic caused by phytophthora, and provides a molecular target for research and development of novel bactericides in the future. The corresponding ADF inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora sojae, phytophthora capsici and phytophthora infestans. The protein provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention belongs to the technical field of modern agriculture, and particularly relates to application of demethylzeylasteral (DZL) to preparation of a product for preventing and treating phytophthora. The invention determines the effect of pentacyclic triterpenoid demethylzeylasteral on activity inhibition of phytophthora capsici and other crop phytophthora, and demethylzeylasteral can inhibit phytophthora, sporangium release, zoospore germination and pathogenicity, which indicates that demethylzeylasteral (DZL) has the effect of resisting phytophthora capsici. Therefore, the compound provided by the invention can be used for preparing a small-molecule inhibitor for growth, development and pathopoiesis of phytophthora of crops, is expected to become a potential drug for resisting phytophthora of crops, and has good development and application values.

The invention provides an application of a CaBBX2 gene in improving the resistance of plants to epidemic diseases, and belongs to the field of plant genetic engineering. The invention finds that overexpression of CaBBX2 can have an obvious inhibition effect on phytophthora of invasive plants. The method provides a new technical approach for plant disease-resistant breeding.

The invention relates to a Tarromyces sp. Strain and application thereof, the Tarromyces sp. Strain is named as Tarromyces sp. Strain F19, the preservation number is GDMCC No: 65365, and the preservation date is October 28, 2024. The Tarromyces sp. Strain is named as Tarromyces sp. Strain F19, the preservation number is GDMCC No: 65365, and the preservation date is October 28, 2024. According to the invention, a new strain of cyanobacteria with phytopathogen resisting and plant growth promoting effects is separated from rhizosphere soil of corn and is preserved, and the strain has a remarkable inhibition effect on various phytopathogens such as fusarium oxysporum, phytophthora nicotianae or Kleiella solani, and can be used as a biocontrol bacterium for preventing and treating various plant diseases; in addition, the strain can also be used for plant growth promotion. The strain resources of the biocontrol bacteria are enriched, and the method can be applied to the development of biopesticides.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus amyloliquefaciens BA-89 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36498. The strain can secrete multiple extracellular enzymes (including protease, amylase, cellulase and glucanase), has the growth promoting characteristics of producing ammonia, dissolving potassium and the like, and can tolerate high temperature and ultraviolet stress; the compound has a remarkable antagonistic effect on various plant pathogenic bacteria (such as verticillium dahliae, fusarium oxysporum and phytophthora capsici); by inoculating the strain on cotton, the plant growth can be obviously promoted, the plant height, stem diameter, root length, leaf area and biomass are improved, and meanwhile, the disease index of verticillium wilt and the colonization amount of pathogenic bacteria at the root are effectively reduced. The strain disclosed by the invention can be used as a biological fertilizer and a biological pesticide, is used for cotton cultivation and disease prevention and control, and has a wide application prospect.

The invention provides a phytophthora nicotianae biocontrol actinomycete FK-1 and application thereof, and belongs to the technical field of biocontrol bacteria. The invention provides a streptomyces strain FK-1, and the preservation number of the streptomyces strain FK-1 is CCTCC (China Center for Type Culture Collection) NO: M 20242872. The streptomyces FK-1 disclosed by the invention shows remarkable broad-spectrum antibacterial activity on various phytopathogens, and has the capabilities of slowing down pathogen infection and reducing plant diseases. The inhibition rate of the streptomyces FK-1 to phytophthora nicotianae reaches 82.08%, the antibacterial activity of strain fermentation liquor can reach 85.88%, and the prevention and treatment effect of indoor potting is 80.65%; meanwhile, the phytophthora nicotianae hyphae can be deformed, wound and irregularly bent, so that the growth of the phytophthora nicotianae hyphae is inhibited, and the method can be applied to prevention and control of phytophthora nicotianae.

The invention belongs to the technical field of plant molecular biology, and particularly discloses immunoregulation GmNFR5a protein and application thereof in plant disease resistance. According to the invention, the function of cell membrane protein in soybean is analyzed, and the immunoregulation protein GmNFR5a is found to play a key role in identifying chitosan oligosaccharide or chitosan oligosaccharide secreted by phytophthora or fungi and play a decisive role in induced immunoreaction in plants. As important commercial crops and food crops, the research on GmNFR5a in soybean can promote the related research on cell membrane receptors of other plants such as pepper, tomato, potato, alfalfa and the like. The researches can more deeply reveal the disease-resistant mechanism of plants to phytophthora and other pathogenic bacteria, and provide high-quality disease-resistant gene resources for disease-resistant gene engineering breeding.

The invention belongs to the technical field of gene engineering, and relates to an StSAM-MT gene and application thereof. The invention provides a StSAM-MT gene. The StSAM-MT gene comprises the following steps: a1) encoding a StSAM-MT protein; and b1) has a nucleotide sequence as shown in SEQ ID NO: 2. The invention reveals that the StSAM-MT gene plays an important role in negative regulation of the resistance of potatoes to phytophthora infestans, provides a theoretical basis and a new technical thought for identification of potato susceptible genes, and has important guiding significance for creating potato germplasm with broad-spectrum resistance and stable yield by accurately controlling the expression of the genes.

The invention belongs to the technical field of fermentation engineering and microbial disease control, and particularly relates to bacillus velezensis XZT106 and application thereof. The invention provides bacillus velezensis XZT106, the preservation number of the bacillus velezensis XZT106 is CCTCC (China Center For Type Culture Collection) M 2025518, and tests show that the bacillus velezensis XZT106 can be used for treating phellinus noxius, botrytis cinerea, colletotrichum gloeosporioides, alternaria tenuissima, fusarium oxysporum, phytophthora capsici and fusarium solanum s.

The invention discloses a litchi immunity related gene LcGLO1 as well as an encoding protein and application thereof. A litchi immunity related gene LcGLO1 is separated from litchi, the full-length cDNA of the gene is 1107 bp, and the nucleotide sequence of the gene is shown as SEQ ID NO: 1; the protein coded by the gene is a lipid transfer protein with the full length consisting of 368 amino acids, and the amino acid sequence is shown as SEQ ID NO: 2. Transcription pattern analysis and plant expression analysis find that the LcGLO1 gene can improve the resistance of plants to phytophthora, so that the litchi immune related gene LcGLO1 can be applied to the aspect of improving the disease resistance of the plants or breeding disease-resistant plants.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention provides a disease-resistant and growth-promoting aspergillus ochraceus AF-P9 and application thereof, and belongs to the technical field of microorganisms. The aspergillus ochraceus AF-P9 provided by the invention has salt resistance and alkali resistance, has the capabilities of producing siderophores and secreting chitinase, and has an efficient inhibition effect on a plurality of pathogenic bacteria such as fusarium oxysporum, fusarium oxysporum, fusarium tripteroides, pythium miscanthus, avena minuta, phomopsis divaricata, sclerotinia sclerotiorum, phytophthora and the like; the composition can be applied to prevention and treatment of rice damping-off. Furthermore, the metabolite of the aspergillus ochraceus AF-P9 is analyzed to obtain that the aspergillus ochraceus AF-P9 can generate a tricyclic cyclohexanone compound, a cyclohexene compound with a hydroxyl group, a phenanthrone-like compound with a hydroxyl group, a benzene ring compound with two long-chain esters, a cyclohexene compound with four parallel ring structures and the like. The aspergillus ochraceus AF-P9 provided by the invention provides a strain resource for biological prevention and control of diseases.

The application belongs to the field of enzyme genetic engineering, and particularly relates to application of a sesquiterpene synthase and a mutant thereof in synthesis of enantiomeric-drimenol compounds, wherein the sesquiterpene synthase is a sesquiterpene synthase from Chenopodium quinoa Willd., and includes CqDMDS1 or CqDMDS2; the mutant of the sesquiterpene synthase is a mutant CqDMDS1 in which an amino acid at position 443 of CqDMDS1 is mutated F443A / G The application realizes heterologous expression of the sesquiterpene synthase and the mutant thereof from Chenopodium quinoa Willd. in an Escherichia coli and a N. benthamiana expression system, catalyzes synthesis of (-)‑8,11-driman-diol or (+)‑drimenol. It is found through activity test that the sesquiterpene synthase from Chenopodium quinoa Willd. has a significant inhibitory effect on plant pathogenic fungi Phytophthora nicotianae, Phytophthora sojae and Fusarium graminearum. The sesquiterpene synthase and the mutant thereof and the biological preparation of the catalytic product have important significance for development of biological pesticides.

The present application provides a novel structure of thiophene compound, the compound has significant inhibitory effect on spore germination and appressorium formation of various plant pathogenic fungi and oomycetes (such as Pyricularia oryzae, Colletotrichum gloeosporioides, Gibberella, Phytophthora, Phytophthora capsici), which can be used for preventing and treating plant diseases caused by fungi and / or oomycetes.

The invention provides a spathiphyllum conifer strain R3, a complex microbial inoculant thereof and application of the spathiphyllum conifer strain R3 in prevention and control of late blight, and belongs to the technical field of biocontrol bacteria. The invention relates to a spathiphylli strain R3, and the preservation number of the spathiphylli strain R3 is CGMCC (China General Microbiological Culture Collection Center) No.36025. The spathiphylli strain R3 has the advantages that the preservation number is CGMCC No.36025; the bacterial strain R3 has good bacteriostatic activity on phytophthora infestans, and meanwhile, the biocontrol agent formed by compounding the bacterial strain R3 with at least one of the bacillus safensis bacterial strain B1 and the leisei bacterial strains L1 and L3 or the combination of the bacterial strain R3 and the L6 has good prevention and control effects in pot experiments and field experiments.

The invention belongs to the technical field of green biological prevention and control of plant diseases, and particularly relates to a biopesticide based on coumarin metabolites and application of the biopesticide in green prevention and control of litchi downy blight. A novel litchi downy blight prevention and control method is developed based on coumarin compounds, and the method passes through in-vitro bacteriostasis experiments, fresh leaf bacteriostasis experiments and comprehensive evaluation of the prevention and control effect. According to the present invention, it is found that the three coumarins such as Scopoletin, Esculin and Esculin can significantly inhibit the growth of peronophythora litchii on a culture medium and a living body leaf, and have good prevention and control effects, and the Scopoletin and Esculin have the most significant antibacterial effect, and can synergistically enhance the inhibition effect on peronophythora litchii, such that the Scopoletin, Esculin and Esculin can significantly inhibit the growth of peronophythora litchii. The method has the advantages of environmental protection, high efficiency, safety and economy, is suitable for large-scale application, and provides a new solution for the prevention and treatment of the litchi downy blight.

This invention provides a gene REL for enhancing plant disease resistance and its applications, belonging to the fields of plant molecular biology and plant genetic engineering. This invention relates to a plant-derived disease resistance gene REL, its recombinant expression vector, and its applications. The gene is derived from *Nicotiana benthamiana* and has the nucleotide sequence shown in SEQ ID NO.1, with the encoded product having the amino acid sequence shown in SEQ ID NO.3. This invention also provides a recombinant expression vector comprising the gene REL described herein. This gene plays a crucial role in tobacco resistance to various *Phytophthora* fungi. This invention can be applied to crop breeding for disease resistance improvement.

The present invention belongs to the field of molecular biology technology, and specifically relates to a plant immune-inducing protein EqBPIE1 and its application. The amino acid sequence of the protein EqBPIE1 is shown in SEQ ID No. 1. The protein EqBPIE1 provided by the present invention can enhance the plant's own immune system from the plant itself, thereby enabling the plant to cope with more diverse environmental changes and infection by multiple pathogens; it has an inhibitory effect on the infection of multiple pathogens on plants, covering multiple pathogens such as rubber tree powdery mildew, Siamese anthracnose, gray mold, Pseudomonas syringae, pepper phytophthora, and soybean phytophthora, and can significantly improve plant disease resistance. The antibacterial effect can be produced at a concentration of 0.1 to 1 μM.

The invention discloses pseudomonas aeruginosa, which is pseudomonas aeruginosa EB-Q03-02, the preservation number of the pseudomonas aeruginosa EB-Q03-02 is CGMCC (China General Microbiological Culture Collection Center) No.30779, the preservation number of the pseudomonas aeruginosa EB-Q03-02 is CGMCC No. 30779, and the preservation number of the pseudomonas aeruginosa EB-Q03-02 is CGMCC No. 30779. The pseudomonas aeruginosa is applied to prevention and treatment of pyrenospora cinerea, helminthosporium planhilum and phytophthora nicotianae. The pseudomonas aeruginosa EB-Q03-02 is separated from a healthy passion fruit root system, has an antagonistic effect on the passion fruit epidemic disease, and particularly has an obvious antagonistic effect on preventing and treating the passion fruit epidemic disease caused by phytophthora nicotianae; furthermore, the pseudomonas aeruginosa EB-Q03-02 disclosed by the invention can be used for effectively inhibiting pyrenospora cinerea, helminthosporium planhilum and phytophthora nicotianae, and the inhibition rate is up to 81% or above.

The invention relates to the technical field of seed treatment, in particular to a soybean seed coating agent as well as a preparation method and application thereof. Each 1kg of soybean seeds in the coating agent comprise the following components in parts by weight: 0.4-0.6 g of naringenin and 7-12g of auxiliary materials; the auxiliary material comprises one or more of a film-forming agent, an adhesive and a filling agent; the film-forming agent is polyvinyl alcohol, the adhesive is Arabic gum, and the filler is talcum powder. The invention further provides a preparation method of the soybean seed coating agent and application of the soybean seed coating agent in prevention and treatment of soybean root rot. The seed coating agent has the advantages that naringenin is used as a core active component, a uniform protective film is formed on the surface of a coated seed, the seed coating agent has double effects of bacteriostasis and crop protection, mycelial growth of main pathogenic bacteria such as soybean root rot such as phytophthora sojae and fusarium oxysporum can be remarkably inhibited, and the resistance of soybeans to pathogenic bacteria of different physiological races is improved. The coating agent is safe and environment-friendly, simple in preparation process and convenient to use, and meets the large-scale planting requirements of soybeans.

The invention provides bacillus and application of the bacillus in inhibition of pathogenic fungi. The invention discloses a novel bacillus (Bacillus) for the first time, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.28299, which is called TG1-2 for short. The bacterial strain provided by the invention is separated from rhizosphere soil of weeds, and is bacillus amyloliquefaciens (Bacillus amyloliquefaciens). Detection shows that the bacterium and fermentation liquor thereof have excellent inhibition effects on various phytopathogens, such as verticillium dahliae, magnaporthe oryzae, fusarium graminearum, botrytis cinerea, sclerotinia sclerotiorum and phytophthora. The bacilli disclosed by the invention have broad-spectrum bacteriostatic action and excellent inhibition effect.

The invention belongs to the technical field of modern agriculture, and particularly relates to a kit for detecting durian root rot phytophthora palmivora and application of the kit. According to the invention, primers and crRNA are designed aiming at a specific sequence of phytophthora palmivora, and a rapid visual detection reagent and a kit for the durian root rot phytophthora palmivora are developed. The reagent or the kit can be used for detecting the durian root rot phytophthora palmivora within 30 minutes, has good specificity and no cross reaction, and has the sensitivity of 10 fg / mu L. In addition, after RPA amplification is finished, a cover does not need to be opened, and a CRISPR / Cas12a reaction system is added, so that aerosol pollution caused by cover opening is avoided. The method does not need to use large instruments and equipment, has the characteristics of short time consumption, simplicity and convenience in operation, high sensitivity, high specificity and the like, and is suitable for rapid visual detection of clinical samples and sites.

Disclosed is a use of a glycine max gene Glyma.13G252100 in regulating glycine max resistance to phytophthora sojae. The nucleotide sequence of the glycine max gene Glyma.13G252100 is as shown in SEQ ID NO: 1. The expression level of the glycine max gene Glyma.13G252100 is reduced to enhance the glycine max resistance to phytophthora sojae; or the expression level of the glycine max gene Glyma.13G252100 is increased to reduce the glycine max resistance to phytophthora sojae. This study is the first to demonstrate that the glycine max resistance to phytophthora sojae can be regulated by modulating the expression of the Glyma.13G252100 gene.

The invention provides application of rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus It is found for the first time that the rhizopus microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Microsporus var. Moreover, the strain has the capabilities of producing cellulase, fixing nitrogen, decomposing inorganic phosphorus and producing siderophores, and pot experiments show that the strain can promote the growth of peppers. The invention provides a new way for biological prevention and control of pepper phytophthora blight, and has positive significance for reducing the use of pesticides and improving the yield and quality of pepper.

The invention provides a disease-resistant and growth-promoting aspergillus alabamate AF-Q5, a microbial preparation and application of the microbial preparation, and belongs to the technical field of microorganisms. The invention provides aspergillus alabamaensis AF-Q5, and the preservation number of the aspergillus alabamaensis AF-Q5 is CGMCC (China General Microbiological Culture Collection Center) No. 41805. The aspergillus alabamaensis AF-Q5 can be used for producing cellulase and has the capability of decomposing cellulose; the bactericidal composition has the inhibiting ability on pathogenic bacteria such as fusarium oxysporum, Adiplomonas minuta, fusarium oxysporum, phomopsis and phytophthora, can be applied to prevention and treatment of rice seedling blight and / or tomato fusarium wilt, can also promote growth of rice and / or tomatoes, and is beneficial to increase of the yield of rice and / or tomatoes and the like.