30 results about "Pseudomonas syringae" patented technology

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

Agricultural compositions are disclosed which comprise at least one isolated bacteriophage capable of infecting the plant pathogen Pseudomonas syringae pv. tomato, the at least one bacteriophage having a genomic nucleic acid sequence at least 85% identical to one of the nucleic acid sequence as set forth in SEQ ID NOs: 1-23. The composition comprises no more than 10 different strains of bacteriophage. Uses thereof for treating bacterial speck disease are also disclosed.

The invention discloses a strain of parateratomycosis aveosphaeroides and application thereof, and belongs to the technical field of microorganism and biological prevention and control. In order to screen new species of fungi and provide alternative strains for application of plant beneficial bacteria in agriculture, a fungus SGSF1293 is separated, and the fungus SGSF1293 is identified to be a new species of the cystis clavatum and is named as the parateratosis aveoensis, and the new species of the cystis clavatum is named as the parateratosis aveoensis. According to the present invention, the activity research results show that the strain SGSF1293 has rice root-knot nematode killing activity, plant pathogen resistance (Brassica oleracea black rot xanthomonas, Pseudomonas syringae, Ralstonia solanacearum and Fusarium oxysporum) resistance, oxidation resistance and phosphorus solubilizing activity; besides, the secondary metabolite of the strain is separated and identified, two active compounds, namely, wintergreen ambrotic acid and brefeldin A, are separated, and the wintergreen ambrotic acid has the activity of killing rice root-knot nematode. The discovery of the strain SGSF1293 and the active metabolite thereof has certain application value in the agricultural fields of plant disease control and the like.

The present application relates to a kind of oligonucleotide primer probe compositions for detecting Pseudomonas syringae, the pathogen of vegetable leaf spot disease based on ERA fluorescence method or ERA test strip method, and the detection method using the composition.The present application can simply, quickly, specifically, sensitively and visually broad-spectrum identify a variety of pathogenic variants of Pseudomonas syringae, the pathogen of vegetable leaf spot disease, the method is easy to operate, reaction time is short, without complex temperature control equipment, can be widely applied to the on-site rapid screening and disease diagnosis of Pseudomonas syringae in vegetable sample.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an agricultural bacteriostatic agent for preventing and treating Pseudomonas syringae and a preparation method of the agricultural bacteriostatic agent. The invention finds that surfactin produced by fermentation of bacillus subtilis has an inhibition effect on agricultural pathogenic bacteria pseudomonas syringae. The invention discloses an IC50 value of surfactin for inhibiting pseudomonas syringae as well as a formula and a preparation method of an agricultural bacteriostatic agent containing the surfactin. The invention provides a new way for preventing and treating pseudomonas syringae plant diseases.

The application discloses a pesticide composition containing silicon-rich rings and application thereof in prevention and treatment of cucumber bacterial angular leaf spot, and belongs to the technical field of biocidal pesticides. Grape seed extract and dracocephalum moldavica extract are obtained by extraction, in indoor tests, the colony diameters of Pseudomonas syringae pv. Lachrymans are reduced, which indicates that the grape seed extract and the dracocephalum moldavica extract can inhibit the growth of Pseudomonas syringae pv. Lachrymans. The two kinds of extracts are used as synergistic extracts, combined with silicon-rich rings and copper oxyquinoline, to prepare a pesticide composition containing silicon-rich rings. In field tests, the disease index of plants is reduced, which indicates that Pseudomonas syringae pv. Lachrymans is inhibited, and the relative control effect of the compound group is the highest, reaching 80.29%. The pesticide composition containing silicon-rich rings prepared by the application can better inhibit Pseudomonas syringae pv. Lachrymans, and is suitable for development of a pesticide for prevention and treatment of cucumber bacterial angular leaf spot.

This invention discloses the application of Panax notoginseng saponin Ft1 as a plant immune inducer in the control of bacterial plant diseases, belonging to the field of biopesticide technology. This invention is the first to discover that Panax notoginseng saponin Ft1 can effectively activate the plant immune system, promote the expression of the FRK1 immune marker gene, induce reactive oxygen species bursts and calcium ion influx, and activate MAPK protein phosphorylation, thereby enhancing plant resistance to bacterial diseases such as *Pseudomonas syringae*. Unlike other existing Panax notoginseng saponins that directly inhibit fungi as fungicides, Panax notoginseng saponin Ft1 works by activating the plant's own immunity and has no inhibitory effect on plant growth at effective concentrations. It is highly efficient, safe, and environmentally friendly, and can be used as a novel biopesticide for the control of bacterial plant diseases.

The invention discloses an application of a broad-spectrum sexual disease resistance gene Ataf1. The invention discloses application of inhibiting expression of an arabidopsis S gene Ataf1 in enhancing resistance of plants to pathogenic bacteria. The CDS sequence of the arabidopsis S gene Ataf1 is shown as SEQ ID NO. 1. According to the invention, an S gene Ataf1 of arabidopsis thaliana is selected, the growth phenotype of a homozygous mutant of the gene constructed by a T-DNA insertion method is observed, and inoculation experiments are carried out on the homozygous mutant by using sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae tomato pathogenic variants and plasmodiophora brassicae. The result shows that the growth of the Ataf1 mutant is not influenced, the Ataf1 mutant shows obvious resistance to sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae and plasmodiophora brassicae, and the scab area and the pathogenic bacterium biomass of the Ataf1 mutant are obviously lower than those of a wild plant. Therefore, the S gene Ataf1 of arabidopsis thaliana can be used as a potential gene resource for a green prevention and control strategy of sclerotinia rot of colza.

The invention discloses an antibacterial peptide with multiple phytopathogen antibacterial activity, a biological antibacterial agent and application of the antibacterial peptide and the biological antibacterial agent, and the amino acid sequence of the antibacterial peptide is FKLIRNLIILRQAWKLIKRRR and is marked as RR-21. Experiments show that the antibacterial peptide disclosed by the invention has a similar alpha-helical structure with a known antibacterial peptide LL-37, and has antibacterial activity on plant pathogenic bacteria represented by pseudomonas syringae and plant pathogenic fungi represented by fusarium pseudograminearum and fusarium oxysporum; compared with the LL-37 polypeptide, the MIC of the RR-21 is 4-8 [mu] g / mL, and the RR-21 has a more significant bacteriostatic effect; meanwhile, under the conventional bacteriostatic concentration (less than or equal to 16 mu g / mL), the RR-21 shows no hemolytic activity; the above fully proves that the antibacterial peptide has high biological safety to eukaryotic cells while exerting multiple antibacterial effects, and can be used as an active component of a biological antibacterial agent, and the biological antibacterial agent has the potential of being developed into an efficient and safe plant disease resistant drug or a plant green pesticide.

The invention discloses a pseudomonas syringae phage as well as a composition and application thereof, and belongs to the technical field of biological prevention and control. The pseudomonas syringae bacteriophage PhiPs-1 which is short in incubation period, short in outbreak period, large in splitting quantity, high in titer, wide in host spectrum, acid-resistant, heat-resistant, chloroform-resistant and ultraviolet-resistant is obtained through screening, and the preservation number of the pseudomonas syringae bacteriophage PhiPs-1 is CCTCC NO: M 20252347; the composition can be developed into a medicinal preparation for preventing and treating the tomato bacterial leaf spot disease; according to the invention, the pseudomonas syringae phage phi Ps-1 and the biocontrol bacterium NBL-AP73 are combined for use, so that the control effect on the tomato bacterial leaf spot disease is synergistically improved.

The invention discloses an application of FLOE2 protein and FLOE3 protein in regulation and control of plant disease resistance. A floe2 single mutant and a floe3 single mutant are hybridized to obtain a floe23 double-mutant material in which both an FLOE2 gene and an FLOE3 gene are mutated, and the analysis on the disease resistance of the floe23 double-mutant material shows that compared with wild arabidopsis thaliana, when the floe23 double-mutant material is infected by pseudomonas syringae carrying Hopz1 effect protein, the disease resistance of the floe23 double-mutant material is remarkably improved, and the disease resistance of the floe23 double-mutant material is remarkably improved. The resistance to pseudomonas syringae is obviously reduced. It is shown that the FLOE2 protein and the FLOE3 protein or biological materials related to the FLOE2 protein and the FLOE3 protein can regulate and control the resistance of plants to the pseudomonas syringae induced by the Hopz1 effect protein and can be used for cultivating disease-resistant plant varieties in agricultural production. The invention is of great significance to the cultivation of disease-resistant plant varieties.

The application discloses application of florfenicol in prevention and treatment of plant bacterial diseases and an inhibitor. The application provides a new application of florfenicol, which can be used for preventing and treating Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. oryzae, Xanthomonas axonopodis pv. citri, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lactucae, Pseudomonas syringae pv. actinidiae, Pseudomonas syringae pv. angulata and the like, and provides a new idea for preventing and treating the above-mentioned plant bacterial diseases. Experiments prove that florfenicol has high bactericidal activity on the above-mentioned plant bacterial diseases.

The invention discloses pseudomonas syringae and an application of the pseudomonas syringae in repairing bone cultural relics. The preservation number of the pseudomonas syringae strain SARIP-03 is CCTCC (China Center for Type Culture Collection) NO: M 20251626, and the preservation number of the pseudomonas syringae strain SARIP-03 is CCTCC NO: M 20251626. The invention further provides a biological agent containing the pseudomonas syringae strain, application of the biological agent in induction of calcium carbonate deposition and solidification and / or reinforcement and repair of bone cultural relics, and a method for reinforcing and repairing the bone cultural relics. The screened pseudomonas syringae strain has high urease activity and carbonic anhydrase activity, when the strain or a biological agent is adopted to induce calcium carbonate deposition, the generation amount of calcium carbonate is high, the compressive strength of bone cultural relics can be improved, and the pseudomonas syringae strain can be widely applied to multiple fields of cultural relic protection, building material reinforcement and the like.

The invention provides an eukaryotic recombinant plasmid and application thereof, relates to the field of plant genetic engineering, and discloses an eukaryotic recombinant plasmid, the eukaryotic recombinant plasmid is an SlTCP12 constitutive CRISPR / Cas9 gene knockout vector, the SlTCP12 constitutive CRISPR / Cas9 gene knockout vector is formed by connecting a TCP12 gene knockout target spot and a plant gene editing vector pKSE401, and the TCP12 gene knockout target spot is connected with the plant gene editing vector pKSE401. The invention further discloses a preparation method and application. The invention provides a novel eukaryotic recombinant plasmid for improving the resistance of tomatoes to pseudomonas syringae, and the eukaryotic recombinant plasmid can reduce tomato leaf scab, reduce the number of infected colonies and increase the expression quantity of immune related genes, and can enhance the antibacterial ability of plants, thereby enhancing the immune response of tomato plants to pathogens of the bacteria. The gene used in the invention is the gene of the tomato, and the finally obtained transgenic tomato has high safety performance.

The invention discloses a rhamnolipid composition for inhibiting Pseudomonas syringae (Pseudomonas syringae) and an application of the rhamnolipid composition, and relates to the technical field of agricultural biocontrol. Experiments show that rhamnolipid has anti-Pseudomonas syringae activity, and the lowest concentration of rhamnolipid in inhibiting Pseudomonas syringae is disclosed. The main active ingredient of the composition is rhamnolipid derived from microorganisms, and the composition further comprises other auxiliaries. The rhamnolipid composition disclosed by the invention has high antibacterial activity on pseudomonas syringae, is simple and convenient to prepare, is economical and green, and has an application value in the aspect of preventing and treating crop diseases caused by pseudomonas syringae.

The application relates to the technical field of agricultural fertilizers, and provides an amino sugar-oligosaccharide biofertilizer and a preparation method thereof. The amino sugar-oligosaccharide biofertilizer comprises 3-4 parts of amino sugar, 12-20 parts of seaweed oligosaccharide preparation liquid, 0.5-1 part of bacillus subtilis, 1-2 parts of trichoderma viride, 1-2 parts of aspergillus niger and 70-83 parts of base material. The seaweed oligosaccharide preparation liquid contained in the amino sugar-oligosaccharide biofertilizer is prepared by an acid enzymolysis-alkaline enzymolysis-biological fermentation three-step method, the content of seaweed oligosaccharide with a stable polymerization degree of 2-5 is high, the content of salicylic acid in crops can be effectively improved, the immune induction effect is obvious, the biocontrol and growth promotion effects of amino sugar and microorganisms are combined, the defense ability of crops against diseases is improved, and especially the growth and reproduction of pathogenic bacteria Pseudomonas syringae pv. tomato can be effectively inhibited, and the occurrence rate of bacterial spot disease of tomatoes can be reduced.

The application discloses a kind of colorectal cancer plasma circulating microbial markers, reagent, kit and its application, belong to biomedical detection technical field.The colorectal cancer plasma circulating microbial markers of the present application include alternaria alternata, xanthomonas campestris, clostridium perfringens, human rumblus, pseudomonas syringae, alternaria alternata, parabacillus licheniformis and pseudomonas szechuanensis.The colorectal cancer plasma circulating microbial markers of the present application can be used for early screening of colorectal cancer, with high sensitivity and specificity, significantly improve the accuracy and reliability of detection;Break through the limitation of traditional ctDNA detection, improve the detection rate of early colorectal cancer.Through low-depth whole genome sequencing, the colorectal cancer plasma circulating microbial markers in colorectal cancer plasma sample are detected, the requirement for depth in experiment is reduced, while ensuring high sensitivity and specificity, cost can be greatly reduced, more suitable for large-scale screening.

The application discloses a tobacco Pseudomonas syringae bacteriophage YL1 and application thereof, and belongs to the technical field of biological control. The bacteriophage can specifically lyse the tobacco Pseudomonas syringae. The bacteriophage has the highest activity and the strongest lysis ability in the range of 30-60 DEG C and pH = 4-11, and has a wide lysis spectrum. Indoor test shows that the lysis rate of the bacteriophage to 23 strains of Pseudomonas syringae reaches 86.96%, and greenhouse potting test shows that the control effect of the bacteriophage reaches 90.82+ / -2.20%, so the bacteriophage has a good biocontrol effect on the tobacco Pseudomonas syringae.

The invention discloses an application of a broad-spectrum sexual disease resistance gene Atbisr6. The knockout arabidopsis Atbisr6 gene is applied to improvement of the resistance of plants to pathogenic bacteria. A homozygous mutant plant of the gene is obtained by screening and identifying a mutant of the gene constructed by a T-DNA insertion method, the growth phenotype of the mutant plant is observed, and inoculation experiments are performed on the mutant plant by using sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae tomato pathogenic variants and plasmodiophora brassicae. The result shows that the growth of the Atbisr6 mutant is not influenced, the Atbisr6 mutant has obvious resistance to sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae and plasmodiophora brassicae, and the scab area and the pathogenic bacterium biomass of the Atbisr6 mutant are obviously lower than those of a wild plant. Therefore, the S gene Atbis6 of arabidopsis thaliana can be used as a potential gene resource, and is applied to a green prevention and control strategy of sclerotinia rot of colza by knocking out the modified gene or inhibiting the expression of the modified gene.

The invention discloses an arabidopsis thaliana WAKL10 gene and application of cell wall-like associated protein kinase WAKL10 coded by the arabidopsis thaliana WAKL10 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana WAKL10 gene in improving the disease resistance of plants, the nucleotide sequence of the arabidopsis thaliana WAKL10 gene is shown as SEQ ID NO.1, the amino acid sequence of cell wall-like associated protein kinase WAKL10 is shown as SEQ ID NO.2, and the plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. It is clear that the Arabidopsis thaliana WAKL10 gene and the cell-wall-like associated protein kinase WAKL10 coded by the Arabidopsis thaliana WAKL10 gene can remarkably enhance the infection resistance of plants to pseudomonas syringae for the first time, the defense capacity of the plants to immunity triggered by a bacterial flagellin induction mode is enhanced, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention relates to the technical field of chemical synthesis and application, in particular to a 1, 4-naphthoquinone derivative and a preparation method and application thereof. The 1, 4-naphthoquinone derivative is prepared by carrying out mercaptan addition on sulfydryl of a sulfydryl-containing compound and 1, 4-naphthoquinone at a site 2, the preparation method is simple, antibacterial activity results show that the prepared 1, 4-naphthoquinone derivative has antibacterial activity on xanthomonas oryzae, pseudomonas syringae and ralstonia solanacearum, and the antibacterial effect is higher than that of kasugamycin.

The invention discloses a microbial bactericide and application thereof. The microbial bactericide comprises the following components: a functional microbial complex microbial inoculant, a targeting bacteriophage composition, a pH-responsive nanogel carrier and an auxiliary agent, wherein the functional microorganism complex microbial inoculant comprises a bacterial strain which is separated from deep-sea sediments and screened by a plate confrontation method, and the diameter of an inhibition zone of the bacterial strain on at least one target pathogenic bacterium is greater than or equal to 15mm; the targeted bacteriophage composition comprises at least two lytic bacteriophages, and the lytic bacteriophages are used for respectively and specifically splitting different types of pathogenic bacteria including ralstonia solanacearum, pseudomonas syringae and Erwinia; four mechanisms of competitive occupation, antibacterial substance inhibition, bacteriophage lysis and induction system resistance are integrated, and the limitation of a single action mode is broken through.

This invention discloses a sugarcane white streak disease resistance gene. ShPR10 The cloning and application of its promoters belong to the field of bioengineering technology. ShPR10 The nucleotide sequence of the gene is shown in SEQ ID NO: 1, and the amino acid sequence of the protein it encodes is shown in SEQ ID NO: 2. This invention discovers... ShPR10 The gene can respond to Xanthomonas aureus infection stress, during Xanthomonas aureus infection. ShPR10 Gene expression levels increased significantly. Further investigation using a transgenic Arabidopsis system confirmed this. ShPR10 Genes can positively regulate plant disease resistance, that is, by overexpressing genes in plants. ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR This invention aims to improve plant resistance to pathogenic tomato species of *Pseudomonas syringae*. It provides a theoretical basis for elucidating the stress response mechanism of sugarcane to *Xanthomonas syringae* infection and also offers valuable genetic resources for disease-resistant sugarcane breeding.

The invention discloses application of bemisia tabaci salivary protein Bt56 in improving insect resistance and disease resistance of plants, and belongs to the technical field of biopesticides. The method comprises the following steps: connecting a coding gene of the bemisia tabaci salivary protein Bt56 with an expression vector pGEX-4T-1 to construct a recombinant vector pGEX-4T-1-Bt56; and expressing the Bt56 protein by using an escherichia coli expression system, and purifying to obtain the plant immune resistance inducer containing the bemisia tabaci salivary protein Bt56. When the plant immune resistance inducer is sprayed on the surfaces of plant leaves, the survival rate and reproduction rate of bemisia tabaci and cotton aphids on plants such as tobacco and the like can be remarkably reduced; the total number of bacterial colonies of pseudomonas syringae on plants such as tobacco and the lesion degree of parasitic phytophthora are obviously reduced. Therefore, the bemisia tabaci salivary protein Bt56 or related biological materials thereof can be used for improving the insect resistance and disease resistance of plants and carrying out green prevention and control on plant diseases and insect pests.

The application discloses a Pseudomonas syringae and application of the Pseudomonas syringae in repairing bone relics. (Pseudomonas syringae) The application also provides a biological bacterial agent containing the Pseudomonas syringae strain and application of the biological bacterial agent in inducing calcium carbonate deposition solidification and / or bone relic reinforcement and repair, and a bone relic reinforcement and repair method. The Pseudomonas syringae strain screened in the application has high urease enzyme activity and carbonic anhydrase enzyme activity, the calcium carbonate generation amount is high when the strain or the biological bacterial agent is used to induce calcium carbonate deposition, and the bone relic compression strength can be improved, so that the Pseudomonas syringae strain can be widely applied in many fields such as cultural relic protection and building material reinforcement.

The application discloses a synergistic composite biological fungicide for preventing and treating cranberry fruit rot and application thereof, and belongs to the technical field of biological prevention preparations. Pseudomonas syringae and benzene ether carbendazol are scientifically compounded, the action mechanisms of the two are completely different, and the two complement each other. Field tests prove that the prevention and treatment effect of the composition on cranberry fruit rot is significantly higher than that of the single use of two single agents, and is also superior to mainstream chemical agents (such as azoxystrobin and mancozeb) on the market at present, and the composition shows obvious synergistic effect. The actually measured prevention and treatment effect can reach more than 75%, and the composition can effectively control the occurrence of diseases.

This invention discloses the application of PACAP peptides as plant immunomodulators, belonging to the field of plant immunology technology. This invention is the first to apply PACAP to a plant system, discovering that it can act as a novel plant immunomodulator to regulate plant immune responses, thereby enhancing plant resistance to pathogenic microorganisms. Experimental results show that PACAP can rapidly induce intracellular calcium ion influx in Arabidopsis thaliana cells, activate the MAPK signaling pathway, and upregulate the expression of early immune marker genes such as FRK1. Furthermore, PACAP treatment significantly improves plant resistance to pathogens such as *Pseudomonas syringae* and *Ralstonia solanacearum*. This invention also confirms that not only does the full-length PACAP (1-38) possess plant immune-promoting activity, but its N-terminal truncated peptide (1-27) and receptor antagonist fragment (6-38) also have plant immune-inducing functions. This invention provides a new technical solution for the development of plant immune inducers.

The present application relates to the technical field of xanthan gum preparation, and particularly relates to an engineered bacterium for producing xanthan gum with low pyruvic acid content, a construction method and application thereof. A wild oilseed rape Pseudomonas syringae is used as a starting strain, and a xps gene is knocked down or knocked out gumL From the genetic regulation level, the degree of pyruvic acid modification of xanthan gum is effectively controlled, and the problem that it is difficult to stably and accurately control the content of pyruvic acid group of xanthan gum in the prior art is solved. The constructed engineered bacterium can stably produce xanthan gum with low pyruvic acid content, the property has good genetic stability and batch consistency, and the product quality fluctuation risk is significantly reduced. Moreover, the construction method is suitable for a wide range of starting strains, under the premise that the growth of the bacterium and the xanthan gum synthesis capacity are not significantly affected, the optimization and regulation of the product structure are realized, and the existing industrial fermentation process can be directly adapted, without the need for additional chemical modification steps, and the method has good industrial application value and promotion prospect.

The application discloses a plant immune induction polypeptide derived from citrus Huanglongbing bacteria and application thereof, wherein the amino acid sequence of the polypeptide is selected from SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3, and the polypeptide also comprises a homologous variant with equivalent immune induction function, and the application also discloses a polynucleotide, a recombination carrier, an engineering strain and an agricultural composition containing the polypeptide, wherein the agricultural composition can be prepared into an aqueous agent, a powder agent or a granular agent, and is applied by spraying, root irrigation or tree trunk injection. Experiments prove that the polypeptide can induce a plant to produce a reactive oxygen burst, activate a MAPK cascade signal path and systematically up-regulate the expression of defense genes such as PR1. The polypeptide can significantly inhibit the colonization and proliferation of pathogenic bacteria such as Pseudomonas syringae in a plant body, has the advantages of high activity, strong specificity and green safety and the like, and provides a new strategy for the prevention and control of plant bacterial diseases.

This invention discloses the application of compound 35C3 in inducing plant immunity and enhancing plant disease resistance, belonging to the field of biopesticide technology. Experiments have shown that compound 35C3 can effectively activate the phosphorylation of plant mitogen-activated protein kinase, induce reactive oxygen species (ROS) bursts, and upregulate the expression levels of disease resistance-related genes FRK1, PR1, PR2, WRKY29, and WRKY53. Pathogen inoculation experiments demonstrated that 35C3 can significantly enhance the resistance of Arabidopsis thaliana to *Pseudomonas syringae* and significantly enhance the resistance of poplar to *Colletotrichum gloeosporioides* and *Botrytis cinerea*. Its mechanism of action is to activate the plant's own immune system rather than directly inhibiting pathogen growth. Furthermore, 35C3 elicits immune responses in various crops, showing broad-spectrum applicability. This invention provides a new approach for developing environmentally friendly biopesticides and has significant agricultural application value.