80 results about "Pseudomonas syringae" patented technology

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention belongs to the technical field of microorganisms, and discloses streptomycete Y309 and application thereof. The preservation number of the streptomyces sp. Y309 is GDMCC No: 64527, and the streptomyces sp. Y309 has relatively strong secondary metabolite synthesis capability and can be used for producing siderophore, IAA (Indoleacetic Acid) and the like. The strain Y309 also has the potential of plant growth promotion and disease control, and the strain Y309 has a relatively strong bacteriostatic effect on 12 pathogenic fungi such as fusarium oxysporum, rhizoctonia cerealis, monilinia fusarium and coracocystis fimbristipula as well as 7 pathogenic bacteria such as pseudomonas syringae lacrimation pathogenic variants and bacillus cereus, and has a relatively strong bacteriostatic effect on 12 pathogenic fungi such as fusarium oxysporum, rhizoctonia cerealis, monilinia fusarium and coracocystis fimbristipula. The strain Y309 has good prevention and treatment effects on wheat sharp eyespot, tomato gray mold and pepper fusarium wilt, fermentation liquor of the strain Y309 can remarkably promote wheat seed germination and seedling growth, agronomic characters such as the plant height, the dry weight and the leaf area in the corn seedling stage can be improved in different saline-alkali soil, and the strain Y309 has good application prospects.

The invention belongs to the technical field of gene engineering, and discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica PGA1 is obtained by carrying out heterologous expression on an arabidopsis thaliana inositol oxygenase gene and a pseudomonas syringae uronic acid dehydrogenase gene in the yarrowia lipolytica by utilizing a genetic engineering technical means. The recombinant yarrowia lipolytica PGA10 capable of efficiently synthesizing glucaric acid (GA) is finally obtained by further modifying six genes of related metabolic pathways of the recombinant yarrowia lipolytica PGA1. The GA yield of the PGA10 reaches 2233.24 mg / L and is 66.27 times that of PGA1, and the effect is remarkable. The invention not only constructs a GA synthesis method, but also defines the influence of related genes such as opi1 on GA synthesis, and provides reference for the research of heterologous synthesis of GA by microorganisms.

The present invention discloses the application of isoquinoline-3-carboxylic acid in preventing and controlling plant bacterial diseases. The isoquinoline-3-carboxylic acid provided by the present invention has an obvious inhibitory effect on plant bacterial diseases, and the plant bacterial diseases are Ralstonia solanacearum, Acidovorax avenae subsp. Citrulli, Pseudomonas syringae pv. actinidiae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzicola, Alternaria brassicae, Xanthomonas citri subsp. citri, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas fragariae, Pseudomonas syringae pv. lachrymans, Dickeya dadantii, Xanthomonas axonopodis pv. mangiferaeindicae. The isoquinoline-3-carboxylic acid of the present invention has protective and therapeutic effects on Acidovorax avenae subsp. Citrulli.

The present invention discloses an application and method of a SPILR protein for improving plant resistance to Pseudomonas syringae, belonging to the field of agricultural science and technology. Specifically, the method comprises using Pseudomonas syringae to induce expression of the SPILR protein in Arabidopsis leaves, constructing an expression vector pCambia1300-SPILR containing the nucleotide sequence of the SPILR protein, transforming the expression vector into Agrobacterium tumefaciens GV3101 competent cells, culturing the cells, and infecting plant leaves to obtain Pseudomonas syringae-resistant plants. Therefore, genetic engineering techniques can be used to cultivate resistant varieties.

Agricultural compositions are disclosed which comprise at least one isolated bacteriophage capable of infecting the plant pathogen Pseudomonas syringae pv. tomato, the at least one bacteriophage having a genomic nucleic acid sequence at least 85% identical to one of the nucleic acid sequence as set forth in SEQ ID NOs: 1-23. The composition comprises no more than 10 different strains of bacteriophage. Uses thereof for treating bacterial speck disease are also disclosed.

The invention discloses an application of a DMP3 gene and an encoding protein thereof in improving plant disease resistance, and belongs to the technical field of gene engineering. The DMP3 gene and the encoding protein thereof are used for improving the disease resistance of plants, the nucleotide sequence of the DMP3 gene is shown as SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the DMP3 gene is shown as SEQ ID NO: 2, especially the disease resistance of the plants to pseudomonas syringae. The invention further provides a PAD3 gene promoter induced by pathogenic bacteria and a plant expression vector containing the PAD3 gene promoter induced by the pathogenic bacteria and a DMP3 gene, wherein the sequence of the PAD3 gene promoter is shown as SEQ ID NO: 3. Real-time quantitative PCR (polymerase chain reaction) shows that Pseudomonas syringae infection remarkably induces up-regulation of expression of a DMP3 gene of a plant, functional experiments show that DMP3 gene expression can activate immune response of the plant, and overexpression of DMP3 causes death of the plant.

The invention belongs to the technical field of microbial engineering, and particularly relates to a polyketone compound extracted from a liquid fermentation product of alternaria alternata as well as a preparation method and application of the polyketone compound. Specifically, the invention discloses two polyketone compounds. The compound Alternariol can be used for inhibiting the growth of bacterial xanthomonas oryzae pv. Oryzae, pseudomonas syringae pv. Kiwifruit pathogenic variant bacteria, staphylococcus aureus and tetracoccus. The compound Dehydroaltenuin can be used for inhibiting the growth of rice bacterial xanthomonas oryzae pv. Oryzae, rice bacterial streak pathogen, tetracoccus, staphylococcus aureus and escherichia coli, and can be used for inhibiting the growth of the rice bacterial xanthomonas oryzae pv. Oryzae.

The invention discloses a strain of parateratomycosis aveosphaeroides and application thereof, and belongs to the technical field of microorganism and biological prevention and control. In order to screen new species of fungi and provide alternative strains for application of plant beneficial bacteria in agriculture, a fungus SGSF1293 is separated, and the fungus SGSF1293 is identified to be a new species of the cystis clavatum and is named as the parateratosis aveoensis, and the new species of the cystis clavatum is named as the parateratosis aveoensis. According to the present invention, the activity research results show that the strain SGSF1293 has rice root-knot nematode killing activity, plant pathogen resistance (Brassica oleracea black rot xanthomonas, Pseudomonas syringae, Ralstonia solanacearum and Fusarium oxysporum) resistance, oxidation resistance and phosphorus solubilizing activity; besides, the secondary metabolite of the strain is separated and identified, two active compounds, namely, wintergreen ambrotic acid and brefeldin A, are separated, and the wintergreen ambrotic acid has the activity of killing rice root-knot nematode. The discovery of the strain SGSF1293 and the active metabolite thereof has certain application value in the agricultural fields of plant disease control and the like.

The present invention relates to new isolated and purified bacteriophages, a bactericidal composition comprising same, and a method for the biocontrol of diseases caused by strains of the species Pseudomonas syringae that effect plant crops, using the bacteriophages and / or composition comprising same.

The present invention belongs to the technical field of plant disease prevention and control, and specifically relates to an agricultural composition of Paenibacillus polymyxa and a gallnut extract and its application. The present invention first discovered that a gallnut extract has an inhibitory effect on the growth of four different pathogens: Pseudomonas syringae pv. kiwifruit, Pseudomonas syringae pv. tomato, Erwinia amylovora, and Xanthomonas citri subsp. citri. The invention also discovered that the gallnut extract has a synergistic effect on Paenibacillus polymyxa. The invention also provides an agricultural composition composed of a gallnut extract and Paenibacillus polymyxa. The agricultural composition has an inhibitory effect on the growth of Pseudomonas syringae pv. kiwifruit, Pseudomonas syringae pv. tomato, Erwinia amylovora, and Xanthomonas citri subsp. citri. The agricultural composition can be applied to the field of plant disease prevention and control.

The present application relates to a kind of oligonucleotide primer probe compositions for detecting Pseudomonas syringae, the pathogen of vegetable leaf spot disease based on ERA fluorescence method or ERA test strip method, and the detection method using the composition.The present application can simply, quickly, specifically, sensitively and visually broad-spectrum identify a variety of pathogenic variants of Pseudomonas syringae, the pathogen of vegetable leaf spot disease, the method is easy to operate, reaction time is short, without complex temperature control equipment, can be widely applied to the on-site rapid screening and disease diagnosis of Pseudomonas syringae in vegetable sample.

The present invention belongs to the field of molecular biology technology, and specifically relates to a plant immune-inducing protein EqBPIE1 and its application. The amino acid sequence of the protein EqBPIE1 is shown in SEQ ID No. 1. The protein EqBPIE1 provided by the present invention can enhance the plant's own immune system from the plant itself, thereby enabling the plant to cope with more diverse environmental changes and infection by multiple pathogens; it has an inhibitory effect on the infection of multiple pathogens on plants, covering multiple pathogens such as rubber tree powdery mildew, Siamese anthracnose, gray mold, Pseudomonas syringae, pepper phytophthora, and soybean phytophthora, and can significantly improve plant disease resistance. The antibacterial effect can be produced at a concentration of 0.1 to 1 μM.

The invention belongs to the technical field of molecular biotechnology and plant genetic engineering, and particularly relates to a grape MYB transcription factor VvMYB108 and application thereof. The invention provides an MYB transcription factor VvMYB108 for regulating and controlling biological stress in a postharvest storage process of grapes. An overexpression strain of the VvMYB108 in arabidopsis thaliana is utilized to find that the sensitivity of a VvMYB108 transgenic strain to botrytis cinerea is enhanced. By using an overexpression strain of the VvMYB108 in arabidopsis thaliana, it is found that the disease resistance of the VvMYB108 transgenic strain to Pst DC3000 is enhanced.

The invention discloses a preparation method and application of an indole C2-position derivative containing a 1, 3, 4-oxadiazole and thioether structure. The compound can be used for effectively preventing and treating bacterial diseases such as kiwi fruit canker pathogen, citrus canker pathogen and rice bacterial blight pathogen. Wherein the EC50 value of the compound 2-((2-fluorophenyl) sulfo)-5-(1H-indol-2-yl)-1, 3, 4-oxadiazole on pathogenic bacteria of the kiwifruit canker is 27.35 mu g / mL, and the prevention and treatment effect is the best; the EC50 values of the compound 2-(1H-indole-2-yl)-5-((4-trifluoromethoxy-phenyl) sulfo)-1, 3, 4-oxadiazole on pathogenic bacteria of citrus canker and pathogenic bacteria of rice bacterial blight are 32.94 mu g / mL and 49.52 mu g / mL respectively, and the control effect is the most ideal. The pesticide composition is obviously superior to control pesticides such as bismerthiazol and thiediazole copper. The indole C2-site derivative containing the 1, 3, 4-oxadiazole and thioether structure can be applied to prevention and treatment of bacterial diseases of crops, and is simple in structure, convenient in preparation process, low in production cost and wide in application prospect.

The invention discloses an application of MEX1 protein and a coding gene thereof in enhancing plant disease resistance. The MEX1 protein is a protein as shown in A1) or A2): A1) a protein composed of an amino acid sequence as shown in SEQ ID No.3 or SEQ ID No.4; a2) a protein which is derived from A1) and is related to improvement of plant disease resistance, wherein the protein is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID No.3 or SEQ ID No.4. The plant MEX1 gene has the effect of negatively regulating the plant disease resistance in tobacco and rice. The gene can be modified by means of gene silencing or gene editing and the like, and the disease resistance of tobacco to potato X virus and clove pseudomonas and the disease resistance of rice to rice stripe virus are improved, so that the yield and quality of crops are increased, and finally actual economic benefits are brought.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an agricultural bacteriostatic agent for preventing and treating Pseudomonas syringae and a preparation method of the agricultural bacteriostatic agent. The invention finds that surfactin produced by fermentation of bacillus subtilis has an inhibition effect on agricultural pathogenic bacteria pseudomonas syringae. The invention discloses an IC50 value of surfactin for inhibiting pseudomonas syringae as well as a formula and a preparation method of an agricultural bacteriostatic agent containing the surfactin. The invention provides a new way for preventing and treating pseudomonas syringae plant diseases.

The invention discloses a bacterial strain for generating coronatine based on a new mechanism, and an efficient breeding method and fermentation application of a coronatine high-yield bacterial strain, and relates to the technical field of bioengineering. The preservation number of the bacterial strain for generating coronatine by a new mechanism is CGMCC (China General Microbiological Culture Collection Center) NO.33248, and the bacterial strain is named as Pseudomonas syringae XZY; the method comprises the following steps: mutagenizing XZY, and performing efficient directional breeding to obtain a coronatine high-yield strain carrying an alpha-ABR phenotype, the preservation number of the coronatine high-yield strain is CGMCC (China General Microbiological Culture Collection Center) NO.30035, and the coronatine high-yield strain is named as Pseudomonas syringae XZY02. The coronatine fermentation yield of the strain is greatly improved compared with the fermentation yield before mutation, and in addition, the invention further provides a brand new method for obtaining the coronatine high-yield strain through directional breeding based on improvement of threonine downstream metabolic flow.

The present invention belongs to the field of microbial technology and discloses Streptomyces sp. Y309 and its application. The Streptomyces Streptomyces sp. Y309, with the preservation number GDMCC No: 64527, has a strong ability to synthesize secondary metabolites and can produce siderophores, IAA, etc. This strain also has the potential for plant growth promotion and disease control. Strain Y309 has strong antibacterial effects on 12 pathogenic fungi such as Fusarium oxysporum, Rhizoctonia cerealis, Monilinia fructicola, and Ceratocystis fimbriata, as well as 7 pathogenic bacteria such as Pseudomonas syringae pv. lachrymans and Bacillus cereus. It has good control effects on wheat sharp eyespot, tomato gray mold, and pepper fusarium wilt. The fermentation broth of strain Y309 can significantly promote the germination of wheat seeds and the growth of seedlings, and can improve the agronomic traits such as plant height, dry weight, and leaf area of maize seedlings in different saline-alkali soils, showing good application prospects.

The application discloses a pesticide composition containing silicon-rich rings and application thereof in prevention and treatment of cucumber bacterial angular leaf spot, and belongs to the technical field of biocidal pesticides. Grape seed extract and dracocephalum moldavica extract are obtained by extraction, in indoor tests, the colony diameters of Pseudomonas syringae pv. Lachrymans are reduced, which indicates that the grape seed extract and the dracocephalum moldavica extract can inhibit the growth of Pseudomonas syringae pv. Lachrymans. The two kinds of extracts are used as synergistic extracts, combined with silicon-rich rings and copper oxyquinoline, to prepare a pesticide composition containing silicon-rich rings. In field tests, the disease index of plants is reduced, which indicates that Pseudomonas syringae pv. Lachrymans is inhibited, and the relative control effect of the compound group is the highest, reaching 80.29%. The pesticide composition containing silicon-rich rings prepared by the application can better inhibit Pseudomonas syringae pv. Lachrymans, and is suitable for development of a pesticide for prevention and treatment of cucumber bacterial angular leaf spot.

This invention discloses the application of Panax notoginseng saponin Ft1 as a plant immune inducer in the control of bacterial plant diseases, belonging to the field of biopesticide technology. This invention is the first to discover that Panax notoginseng saponin Ft1 can effectively activate the plant immune system, promote the expression of the FRK1 immune marker gene, induce reactive oxygen species bursts and calcium ion influx, and activate MAPK protein phosphorylation, thereby enhancing plant resistance to bacterial diseases such as *Pseudomonas syringae*. Unlike other existing Panax notoginseng saponins that directly inhibit fungi as fungicides, Panax notoginseng saponin Ft1 works by activating the plant's own immunity and has no inhibitory effect on plant growth at effective concentrations. It is highly efficient, safe, and environmentally friendly, and can be used as a novel biopesticide for the control of bacterial plant diseases.

The application discloses a preparation method and application of an indole oxadiazole aryl thiourea derivative, and has the structure shown in the following general formula: The compound has a good inhibiting effect on pathogenic bacteria, and has a good inhibiting effect on pathogenic bacteria such as Xanthomonas campestris, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. mori, Xanthomonas axonopodis pv. citri, Xanthomonas axonopodis pv. viticola, Xanthomonas axonopodis pv. vesicatoria, Xanthomonas axonopodis pv. actinidiae, Xanthomonas axonopodis pv. apodrecedens, Botrytis cinerea, Fusarium oxysporum f. sp. cubense, Sclerotinia sclerotiorum, Gibberella zeae, Phytophthora infestans, Monilinia fructicola, and the like, and provides an important scientific basis for research and development of new pesticides.

The invention relates to a pseudomonas syringae kiwifruit pathogenic variant gene knockout vector, a strain, a construction method of the strain and application of the strain in obtaining a transgenic plant. According to the invention, modification is carried out on the basis of an original CPRISPR / FnCas12a system, a genetic manipulation technology using Psa gene editing is constructed, gene knockout is successfully carried out on the three-type effector hopH1, plasmid elimination is realized, and subsequent gene supplementation or continuous knockout of other genes is facilitated. Through screening and verification, a knockout strain deltahopH1 of hopH1, a backfill strain C deltahopH1 of hopH1, a double knockout strain deltahopZ5 deltahopH1 of hopH1 and hopZ5, and a backfill strain C deltahopZ5 deltahopH1 of hopH1 and hopZ5 are obtained. Compared with the prior art, the method overcomes the defects that the knockout period of the pseudomonas syringae kiwifruit pathogenic variant gene is long and the operation is tedious.

The invention discloses an application of a broad-spectrum sexual disease resistance gene Ataf1. The invention discloses application of inhibiting expression of an arabidopsis S gene Ataf1 in enhancing resistance of plants to pathogenic bacteria. The CDS sequence of the arabidopsis S gene Ataf1 is shown as SEQ ID NO. 1. According to the invention, an S gene Ataf1 of arabidopsis thaliana is selected, the growth phenotype of a homozygous mutant of the gene constructed by a T-DNA insertion method is observed, and inoculation experiments are carried out on the homozygous mutant by using sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae tomato pathogenic variants and plasmodiophora brassicae. The result shows that the growth of the Ataf1 mutant is not influenced, the Ataf1 mutant shows obvious resistance to sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae and plasmodiophora brassicae, and the scab area and the pathogenic bacterium biomass of the Ataf1 mutant are obviously lower than those of a wild plant. Therefore, the S gene Ataf1 of arabidopsis thaliana can be used as a potential gene resource for a green prevention and control strategy of sclerotinia rot of colza.

The invention discloses an antibacterial peptide with multiple phytopathogen antibacterial activity, a biological antibacterial agent and application of the antibacterial peptide and the biological antibacterial agent, and the amino acid sequence of the antibacterial peptide is FKLIRNLIILRQAWKLIKRRR and is marked as RR-21. Experiments show that the antibacterial peptide disclosed by the invention has a similar alpha-helical structure with a known antibacterial peptide LL-37, and has antibacterial activity on plant pathogenic bacteria represented by pseudomonas syringae and plant pathogenic fungi represented by fusarium pseudograminearum and fusarium oxysporum; compared with the LL-37 polypeptide, the MIC of the RR-21 is 4-8 [mu] g / mL, and the RR-21 has a more significant bacteriostatic effect; meanwhile, under the conventional bacteriostatic concentration (less than or equal to 16 mu g / mL), the RR-21 shows no hemolytic activity; the above fully proves that the antibacterial peptide has high biological safety to eukaryotic cells while exerting multiple antibacterial effects, and can be used as an active component of a biological antibacterial agent, and the biological antibacterial agent has the potential of being developed into an efficient and safe plant disease resistant drug or a plant green pesticide.

The present disclosure provides an antimicrobial peptide (AMP) for controlling Pseudomonas syringae pv. actinidiae (PSA) and a preparation method and use thereof, belonging to the technical field of biological control. In the present disclosure, the AMP has an amino acid sequence of Trp-Trp-Lys-Leu-Leu-Arg-Lys-Leu-NH2, and has a typical amphiphilic structure. The AMP can target a cell membrane of the PSA, increasing permeabilization and dissipating a membrane potential, which leads to calcium ion leakage. The AMP also acts on intracellular targets, affects DNA functions, reduces an activity of esterases, and induces the generation of reactive oxygen species (ROS). The AMP has a minimal inhibitory concentration (MIC) of 3.13 μg / mL and a half maximal effective concentration (EC50) of 1.67 μg / mL for the PSA, showing an activity significantly higher than that of agricultural streptomycin.

To provide a Pseudomonas syringae-inhibiting composition which contains alcohol but still inhibits the growth of Pseudomonas syringae while inhibiting the production of IL-1α caused by the alcohol.SOLUTION: It was found that a composition which contains a monovalent alcohol having 1-3 carbon atoms and also contains a copolymer (P) having a specific structure can inhibit the growth of Pseudomonas syringae while inhibiting the production of IL-1α. The findings have led to the completion of the present invention.SELECTED DRAWING: None

This invention belongs to the field of genetic engineering technology, specifically relating to the application of the AtHIR4 gene and / or its encoded protein in regulating plant pathogen resistance. The amino acid sequence of the protein encoded by the AtHIR4 gene described in this invention is shown in SEQ ID No. 1. This invention created transgenic Arabidopsis and rapeseed expressing hypersensitivity response-inducible protein 4 (AtHIR4). In the AtHIR4 transgenic Arabidopsis, the chitin-activated ROS burst and phosphorylation of MAPKs were significantly enhanced. Disease resistance test results showed that the AtHIR4 transgenic plants not only exhibited enhanced resistance to the necrotrophic pathogens *Sclerotinia sclerotiorum* and *Botrytis cinerea*, but also significantly increased resistance to the semi-vitrotrophic pathogen *Pseudomonas syringae*. The AtHIR4 transgenic rapeseed also showed significantly enhanced resistance to *Sclerotinia sclerotiorum* and *Botrytis cinerea*.

The invention discloses a pseudomonas syringae phage as well as a composition and application thereof, and belongs to the technical field of biological prevention and control. The pseudomonas syringae bacteriophage PhiPs-1 which is short in incubation period, short in outbreak period, large in splitting quantity, high in titer, wide in host spectrum, acid-resistant, heat-resistant, chloroform-resistant and ultraviolet-resistant is obtained through screening, and the preservation number of the pseudomonas syringae bacteriophage PhiPs-1 is CCTCC NO: M 20252347; the composition can be developed into a medicinal preparation for preventing and treating the tomato bacterial leaf spot disease; according to the invention, the pseudomonas syringae phage phi Ps-1 and the biocontrol bacterium NBL-AP73 are combined for use, so that the control effect on the tomato bacterial leaf spot disease is synergistically improved.