57 results about "Pseudomonas syringae" patented technology

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention belongs to the technical field of gene engineering, and discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica PGA1 is obtained by carrying out heterologous expression on an arabidopsis thaliana inositol oxygenase gene and a pseudomonas syringae uronic acid dehydrogenase gene in the yarrowia lipolytica by utilizing a genetic engineering technical means. The recombinant yarrowia lipolytica PGA10 capable of efficiently synthesizing glucaric acid (GA) is finally obtained by further modifying six genes of related metabolic pathways of the recombinant yarrowia lipolytica PGA1. The GA yield of the PGA10 reaches 2233.24 mg / L and is 66.27 times that of PGA1, and the effect is remarkable. The invention not only constructs a GA synthesis method, but also defines the influence of related genes such as opi1 on GA synthesis, and provides reference for the research of heterologous synthesis of GA by microorganisms.

The present invention discloses an application and method of a SPILR protein for improving plant resistance to Pseudomonas syringae, belonging to the field of agricultural science and technology. Specifically, the method comprises using Pseudomonas syringae to induce expression of the SPILR protein in Arabidopsis leaves, constructing an expression vector pCambia1300-SPILR containing the nucleotide sequence of the SPILR protein, transforming the expression vector into Agrobacterium tumefaciens GV3101 competent cells, culturing the cells, and infecting plant leaves to obtain Pseudomonas syringae-resistant plants. Therefore, genetic engineering techniques can be used to cultivate resistant varieties.

Agricultural compositions are disclosed which comprise at least one isolated bacteriophage capable of infecting the plant pathogen Pseudomonas syringae pv. tomato, the at least one bacteriophage having a genomic nucleic acid sequence at least 85% identical to one of the nucleic acid sequence as set forth in SEQ ID NOs: 1-23. The composition comprises no more than 10 different strains of bacteriophage. Uses thereof for treating bacterial speck disease are also disclosed.

The invention discloses an application of a DMP3 gene and an encoding protein thereof in improving plant disease resistance, and belongs to the technical field of gene engineering. The DMP3 gene and the encoding protein thereof are used for improving the disease resistance of plants, the nucleotide sequence of the DMP3 gene is shown as SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the DMP3 gene is shown as SEQ ID NO: 2, especially the disease resistance of the plants to pseudomonas syringae. The invention further provides a PAD3 gene promoter induced by pathogenic bacteria and a plant expression vector containing the PAD3 gene promoter induced by the pathogenic bacteria and a DMP3 gene, wherein the sequence of the PAD3 gene promoter is shown as SEQ ID NO: 3. Real-time quantitative PCR (polymerase chain reaction) shows that Pseudomonas syringae infection remarkably induces up-regulation of expression of a DMP3 gene of a plant, functional experiments show that DMP3 gene expression can activate immune response of the plant, and overexpression of DMP3 causes death of the plant.

The invention belongs to the technical field of microbial engineering, and particularly relates to a polyketone compound extracted from a liquid fermentation product of alternaria alternata as well as a preparation method and application of the polyketone compound. Specifically, the invention discloses two polyketone compounds. The compound Alternariol can be used for inhibiting the growth of bacterial xanthomonas oryzae pv. Oryzae, pseudomonas syringae pv. Kiwifruit pathogenic variant bacteria, staphylococcus aureus and tetracoccus. The compound Dehydroaltenuin can be used for inhibiting the growth of rice bacterial xanthomonas oryzae pv. Oryzae, rice bacterial streak pathogen, tetracoccus, staphylococcus aureus and escherichia coli, and can be used for inhibiting the growth of the rice bacterial xanthomonas oryzae pv. Oryzae.

The invention discloses a strain of parateratomycosis aveosphaeroides and application thereof, and belongs to the technical field of microorganism and biological prevention and control. In order to screen new species of fungi and provide alternative strains for application of plant beneficial bacteria in agriculture, a fungus SGSF1293 is separated, and the fungus SGSF1293 is identified to be a new species of the cystis clavatum and is named as the parateratosis aveoensis, and the new species of the cystis clavatum is named as the parateratosis aveoensis. According to the present invention, the activity research results show that the strain SGSF1293 has rice root-knot nematode killing activity, plant pathogen resistance (Brassica oleracea black rot xanthomonas, Pseudomonas syringae, Ralstonia solanacearum and Fusarium oxysporum) resistance, oxidation resistance and phosphorus solubilizing activity; besides, the secondary metabolite of the strain is separated and identified, two active compounds, namely, wintergreen ambrotic acid and brefeldin A, are separated, and the wintergreen ambrotic acid has the activity of killing rice root-knot nematode. The discovery of the strain SGSF1293 and the active metabolite thereof has certain application value in the agricultural fields of plant disease control and the like.

The present invention belongs to the technical field of plant disease prevention and control, and specifically relates to an agricultural composition of Paenibacillus polymyxa and a gallnut extract and its application. The present invention first discovered that a gallnut extract has an inhibitory effect on the growth of four different pathogens: Pseudomonas syringae pv. kiwifruit, Pseudomonas syringae pv. tomato, Erwinia amylovora, and Xanthomonas citri subsp. citri. The invention also discovered that the gallnut extract has a synergistic effect on Paenibacillus polymyxa. The invention also provides an agricultural composition composed of a gallnut extract and Paenibacillus polymyxa. The agricultural composition has an inhibitory effect on the growth of Pseudomonas syringae pv. kiwifruit, Pseudomonas syringae pv. tomato, Erwinia amylovora, and Xanthomonas citri subsp. citri. The agricultural composition can be applied to the field of plant disease prevention and control.

The present application relates to a kind of oligonucleotide primer probe compositions for detecting Pseudomonas syringae, the pathogen of vegetable leaf spot disease based on ERA fluorescence method or ERA test strip method, and the detection method using the composition.The present application can simply, quickly, specifically, sensitively and visually broad-spectrum identify a variety of pathogenic variants of Pseudomonas syringae, the pathogen of vegetable leaf spot disease, the method is easy to operate, reaction time is short, without complex temperature control equipment, can be widely applied to the on-site rapid screening and disease diagnosis of Pseudomonas syringae in vegetable sample.

The present invention belongs to the field of molecular biology technology, and specifically relates to a plant immune-inducing protein EqBPIE1 and its application. The amino acid sequence of the protein EqBPIE1 is shown in SEQ ID No. 1. The protein EqBPIE1 provided by the present invention can enhance the plant's own immune system from the plant itself, thereby enabling the plant to cope with more diverse environmental changes and infection by multiple pathogens; it has an inhibitory effect on the infection of multiple pathogens on plants, covering multiple pathogens such as rubber tree powdery mildew, Siamese anthracnose, gray mold, Pseudomonas syringae, pepper phytophthora, and soybean phytophthora, and can significantly improve plant disease resistance. The antibacterial effect can be produced at a concentration of 0.1 to 1 μM.

The invention discloses an application of MEX1 protein and a coding gene thereof in enhancing plant disease resistance. The MEX1 protein is a protein as shown in A1) or A2): A1) a protein composed of an amino acid sequence as shown in SEQ ID No.3 or SEQ ID No.4; a2) a protein which is derived from A1) and is related to improvement of plant disease resistance, wherein the protein is formed by substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID No.3 or SEQ ID No.4. The plant MEX1 gene has the effect of negatively regulating the plant disease resistance in tobacco and rice. The gene can be modified by means of gene silencing or gene editing and the like, and the disease resistance of tobacco to potato X virus and clove pseudomonas and the disease resistance of rice to rice stripe virus are improved, so that the yield and quality of crops are increased, and finally actual economic benefits are brought.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an agricultural bacteriostatic agent for preventing and treating Pseudomonas syringae and a preparation method of the agricultural bacteriostatic agent. The invention finds that surfactin produced by fermentation of bacillus subtilis has an inhibition effect on agricultural pathogenic bacteria pseudomonas syringae. The invention discloses an IC50 value of surfactin for inhibiting pseudomonas syringae as well as a formula and a preparation method of an agricultural bacteriostatic agent containing the surfactin. The invention provides a new way for preventing and treating pseudomonas syringae plant diseases.

The application discloses a pesticide composition containing silicon-rich rings and application thereof in prevention and treatment of cucumber bacterial angular leaf spot, and belongs to the technical field of biocidal pesticides. Grape seed extract and dracocephalum moldavica extract are obtained by extraction, in indoor tests, the colony diameters of Pseudomonas syringae pv. Lachrymans are reduced, which indicates that the grape seed extract and the dracocephalum moldavica extract can inhibit the growth of Pseudomonas syringae pv. Lachrymans. The two kinds of extracts are used as synergistic extracts, combined with silicon-rich rings and copper oxyquinoline, to prepare a pesticide composition containing silicon-rich rings. In field tests, the disease index of plants is reduced, which indicates that Pseudomonas syringae pv. Lachrymans is inhibited, and the relative control effect of the compound group is the highest, reaching 80.29%. The pesticide composition containing silicon-rich rings prepared by the application can better inhibit Pseudomonas syringae pv. Lachrymans, and is suitable for development of a pesticide for prevention and treatment of cucumber bacterial angular leaf spot.

This invention discloses the application of Panax notoginseng saponin Ft1 as a plant immune inducer in the control of bacterial plant diseases, belonging to the field of biopesticide technology. This invention is the first to discover that Panax notoginseng saponin Ft1 can effectively activate the plant immune system, promote the expression of the FRK1 immune marker gene, induce reactive oxygen species bursts and calcium ion influx, and activate MAPK protein phosphorylation, thereby enhancing plant resistance to bacterial diseases such as *Pseudomonas syringae*. Unlike other existing Panax notoginseng saponins that directly inhibit fungi as fungicides, Panax notoginseng saponin Ft1 works by activating the plant's own immunity and has no inhibitory effect on plant growth at effective concentrations. It is highly efficient, safe, and environmentally friendly, and can be used as a novel biopesticide for the control of bacterial plant diseases.

The application discloses a preparation method and application of an indole oxadiazole aryl thiourea derivative, and has the structure shown in the following general formula: The compound has a good inhibiting effect on pathogenic bacteria, and has a good inhibiting effect on pathogenic bacteria such as Xanthomonas campestris, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. mori, Xanthomonas axonopodis pv. citri, Xanthomonas axonopodis pv. viticola, Xanthomonas axonopodis pv. vesicatoria, Xanthomonas axonopodis pv. actinidiae, Xanthomonas axonopodis pv. apodrecedens, Botrytis cinerea, Fusarium oxysporum f. sp. cubense, Sclerotinia sclerotiorum, Gibberella zeae, Phytophthora infestans, Monilinia fructicola, and the like, and provides an important scientific basis for research and development of new pesticides.

The invention relates to a pseudomonas syringae kiwifruit pathogenic variant gene knockout vector, a strain, a construction method of the strain and application of the strain in obtaining a transgenic plant. According to the invention, modification is carried out on the basis of an original CPRISPR / FnCas12a system, a genetic manipulation technology using Psa gene editing is constructed, gene knockout is successfully carried out on the three-type effector hopH1, plasmid elimination is realized, and subsequent gene supplementation or continuous knockout of other genes is facilitated. Through screening and verification, a knockout strain deltahopH1 of hopH1, a backfill strain C deltahopH1 of hopH1, a double knockout strain deltahopZ5 deltahopH1 of hopH1 and hopZ5, and a backfill strain C deltahopZ5 deltahopH1 of hopH1 and hopZ5 are obtained. Compared with the prior art, the method overcomes the defects that the knockout period of the pseudomonas syringae kiwifruit pathogenic variant gene is long and the operation is tedious.

The invention discloses an application of a broad-spectrum sexual disease resistance gene Ataf1. The invention discloses application of inhibiting expression of an arabidopsis S gene Ataf1 in enhancing resistance of plants to pathogenic bacteria. The CDS sequence of the arabidopsis S gene Ataf1 is shown as SEQ ID NO. 1. According to the invention, an S gene Ataf1 of arabidopsis thaliana is selected, the growth phenotype of a homozygous mutant of the gene constructed by a T-DNA insertion method is observed, and inoculation experiments are carried out on the homozygous mutant by using sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae tomato pathogenic variants and plasmodiophora brassicae. The result shows that the growth of the Ataf1 mutant is not influenced, the Ataf1 mutant shows obvious resistance to sclerotinia sclerotiorum, botrytis cinerea, pseudomonas syringae and plasmodiophora brassicae, and the scab area and the pathogenic bacterium biomass of the Ataf1 mutant are obviously lower than those of a wild plant. Therefore, the S gene Ataf1 of arabidopsis thaliana can be used as a potential gene resource for a green prevention and control strategy of sclerotinia rot of colza.

The invention discloses an antibacterial peptide with multiple phytopathogen antibacterial activity, a biological antibacterial agent and application of the antibacterial peptide and the biological antibacterial agent, and the amino acid sequence of the antibacterial peptide is FKLIRNLIILRQAWKLIKRRR and is marked as RR-21. Experiments show that the antibacterial peptide disclosed by the invention has a similar alpha-helical structure with a known antibacterial peptide LL-37, and has antibacterial activity on plant pathogenic bacteria represented by pseudomonas syringae and plant pathogenic fungi represented by fusarium pseudograminearum and fusarium oxysporum; compared with the LL-37 polypeptide, the MIC of the RR-21 is 4-8 [mu] g / mL, and the RR-21 has a more significant bacteriostatic effect; meanwhile, under the conventional bacteriostatic concentration (less than or equal to 16 mu g / mL), the RR-21 shows no hemolytic activity; the above fully proves that the antibacterial peptide has high biological safety to eukaryotic cells while exerting multiple antibacterial effects, and can be used as an active component of a biological antibacterial agent, and the biological antibacterial agent has the potential of being developed into an efficient and safe plant disease resistant drug or a plant green pesticide.

The present disclosure provides an antimicrobial peptide (AMP) for controlling Pseudomonas syringae pv. actinidiae (PSA) and a preparation method and use thereof, belonging to the technical field of biological control. In the present disclosure, the AMP has an amino acid sequence of Trp-Trp-Lys-Leu-Leu-Arg-Lys-Leu-NH2, and has a typical amphiphilic structure. The AMP can target a cell membrane of the PSA, increasing permeabilization and dissipating a membrane potential, which leads to calcium ion leakage. The AMP also acts on intracellular targets, affects DNA functions, reduces an activity of esterases, and induces the generation of reactive oxygen species (ROS). The AMP has a minimal inhibitory concentration (MIC) of 3.13 μg / mL and a half maximal effective concentration (EC50) of 1.67 μg / mL for the PSA, showing an activity significantly higher than that of agricultural streptomycin.

This invention belongs to the field of genetic engineering technology, specifically relating to the application of the AtHIR4 gene and / or its encoded protein in regulating plant pathogen resistance. The amino acid sequence of the protein encoded by the AtHIR4 gene described in this invention is shown in SEQ ID No. 1. This invention created transgenic Arabidopsis and rapeseed expressing hypersensitivity response-inducible protein 4 (AtHIR4). In the AtHIR4 transgenic Arabidopsis, the chitin-activated ROS burst and phosphorylation of MAPKs were significantly enhanced. Disease resistance test results showed that the AtHIR4 transgenic plants not only exhibited enhanced resistance to the necrotrophic pathogens *Sclerotinia sclerotiorum* and *Botrytis cinerea*, but also significantly increased resistance to the semi-vitrotrophic pathogen *Pseudomonas syringae*. The AtHIR4 transgenic rapeseed also showed significantly enhanced resistance to *Sclerotinia sclerotiorum* and *Botrytis cinerea*.

The invention discloses a pseudomonas syringae phage as well as a composition and application thereof, and belongs to the technical field of biological prevention and control. The pseudomonas syringae bacteriophage PhiPs-1 which is short in incubation period, short in outbreak period, large in splitting quantity, high in titer, wide in host spectrum, acid-resistant, heat-resistant, chloroform-resistant and ultraviolet-resistant is obtained through screening, and the preservation number of the pseudomonas syringae bacteriophage PhiPs-1 is CCTCC NO: M 20252347; the composition can be developed into a medicinal preparation for preventing and treating the tomato bacterial leaf spot disease; according to the invention, the pseudomonas syringae phage phi Ps-1 and the biocontrol bacterium NBL-AP73 are combined for use, so that the control effect on the tomato bacterial leaf spot disease is synergistically improved.

The invention discloses an application of FLOE2 protein and FLOE3 protein in regulation and control of plant disease resistance. A floe2 single mutant and a floe3 single mutant are hybridized to obtain a floe23 double-mutant material in which both an FLOE2 gene and an FLOE3 gene are mutated, and the analysis on the disease resistance of the floe23 double-mutant material shows that compared with wild arabidopsis thaliana, when the floe23 double-mutant material is infected by pseudomonas syringae carrying Hopz1 effect protein, the disease resistance of the floe23 double-mutant material is remarkably improved, and the disease resistance of the floe23 double-mutant material is remarkably improved. The resistance to pseudomonas syringae is obviously reduced. It is shown that the FLOE2 protein and the FLOE3 protein or biological materials related to the FLOE2 protein and the FLOE3 protein can regulate and control the resistance of plants to the pseudomonas syringae induced by the Hopz1 effect protein and can be used for cultivating disease-resistant plant varieties in agricultural production. The invention is of great significance to the cultivation of disease-resistant plant varieties.

The application discloses a method for preparing nano-silver particles by using coleus extraction liquid and products and application thereof, and belongs to the technical field of green biosynthesis of nanometer materials, and comprises the following steps: (1) after pretreatment, the coleus is crushed, mixed with deionized water, stirred uniformly, and then allowed to stand, and the coleus extraction liquid is obtained after filtration; (2) the coleus extraction liquid obtained in step (1) is mixed with an AgNO3 solution to obtain a mixed solution, and then the nano-silver particles are obtained after further centrifugation, washing and vacuum freeze-drying. The nano-silver particles obtained by the method have small average particle size, uniform size and stable structure, can effectively inhibit the activity of multiple plant pathogenic bacteria such as Pseudomonas syringae pv. tomato, can destroy the cell structure to make the endosolutes exosmose, has good bactericidal effect, and can be used for preventing and treating bacterial black spot disease of cruciferous vegetables.

The application discloses application of florfenicol in prevention and treatment of plant bacterial diseases and an inhibitor. The application provides a new application of florfenicol, which can be used for preventing and treating Xanthomonas oryzae pv. oryzae, Xanthomonas campestris pv. oryzae, Xanthomonas axonopodis pv. citri, Pseudomonas syringae pv. tabaci, Pseudomonas syringae pv. lachrymans, Pseudomonas syringae pv. tomato, Pseudomonas syringae pv. lactucae, Pseudomonas syringae pv. actinidiae, Pseudomonas syringae pv. angulata and the like, and provides a new idea for preventing and treating the above-mentioned plant bacterial diseases. Experiments prove that florfenicol has high bactericidal activity on the above-mentioned plant bacterial diseases.

The invention relates to the technical field of biology, and discloses MNP marker sites for identifying pseudomonas syringae, a primer composition, a reagent, a kit and application, the MNP marker composition comprises 157 marker sites from MNP-1 to MNP-157, the MNP marker site is a genome region which is screened on a pseudomonas syringae genome, has the specificity of the pseudomonas syringae and has a plurality of nucleotide polymorphisms in different pathogenic variants of the pseudomonas syringae; according to the present invention, the MNP marker site is found, the multiple primer composition is designed according to the found MNP marker site, the multiple PCR amplification can be performed by using the designed multiple primer composition, and the sequencing of the amplification product is performed by fusing with the next-generation sequencing platform, such that the high-throughput, high-efficiency and high-accuracy identification and the high-throughput, high-efficiency and high-accuracy identification and the high-throughput, high-efficiency and high-accuracy identification and the high-throughput, high-efficiency and high-accuracy identification and the high-throughput, high-efficiency and high-accuracy identification and the high-throughput, high-efficiency and high-accuracy identification and the high-efficiency and high-accuracy the technical support is provided for the problems of investigation, identification, prevention and control and the like of P.syringae pathogenic variants.

The invention discloses pseudomonas syringae and an application of the pseudomonas syringae in repairing bone cultural relics. The preservation number of the pseudomonas syringae strain SARIP-03 is CCTCC (China Center for Type Culture Collection) NO: M 20251626, and the preservation number of the pseudomonas syringae strain SARIP-03 is CCTCC NO: M 20251626. The invention further provides a biological agent containing the pseudomonas syringae strain, application of the biological agent in induction of calcium carbonate deposition and solidification and / or reinforcement and repair of bone cultural relics, and a method for reinforcing and repairing the bone cultural relics. The screened pseudomonas syringae strain has high urease activity and carbonic anhydrase activity, when the strain or a biological agent is adopted to induce calcium carbonate deposition, the generation amount of calcium carbonate is high, the compressive strength of bone cultural relics can be improved, and the pseudomonas syringae strain can be widely applied to multiple fields of cultural relic protection, building material reinforcement and the like.

The present invention discloses the development of bacteriophage technology for controlling bacterial diseases caused by phytopathogens. In the development to demonstrate the effectiveness of the method, three bacteriophages from natural sources were selected and their ability to destroy different strains of Pseudomonas syringae was demonstrated. In vitro efficacy tests showed that the bacteriophages eliminated 99% of the bacterial population within an hour. During effectiveness tests in plants, the treatment with bacteriophages showed a preventive and healing effect against bacterial cancer in cherry trees.

The invention provides an eukaryotic recombinant plasmid and application thereof, relates to the field of plant genetic engineering, and discloses an eukaryotic recombinant plasmid, the eukaryotic recombinant plasmid is an SlTCP12 constitutive CRISPR / Cas9 gene knockout vector, the SlTCP12 constitutive CRISPR / Cas9 gene knockout vector is formed by connecting a TCP12 gene knockout target spot and a plant gene editing vector pKSE401, and the TCP12 gene knockout target spot is connected with the plant gene editing vector pKSE401. The invention further discloses a preparation method and application. The invention provides a novel eukaryotic recombinant plasmid for improving the resistance of tomatoes to pseudomonas syringae, and the eukaryotic recombinant plasmid can reduce tomato leaf scab, reduce the number of infected colonies and increase the expression quantity of immune related genes, and can enhance the antibacterial ability of plants, thereby enhancing the immune response of tomato plants to pathogens of the bacteria. The gene used in the invention is the gene of the tomato, and the finally obtained transgenic tomato has high safety performance.

The application provides an anti-disease promoting Aspergillus ochraceus AF-P9 and an application thereof, and belongs to the technical field of microorganisms.The application provides the Aspergillus ochraceus AF-P9, which has salt tolerance and alkali tolerance, has the ability of producing iron carrier and secreting chitinase, has a high efficient inhibiting effect on multiple pathogenic bacteria such as Fusarium oxysporum, Fusarium acuminatum, Fusarium decemcellulare, Pythium aphanidermatum, Pseudomonas syringae pv., Sclerotinia sclerotiorum and Phytophthora, and can be applied to the prevention and treatment of rice damping-off.Further, metabolites of the Aspergillus ochraceus AF-P9 are analyzed, and it is found that the Aspergillus ochraceus AF-P9 can produce tricyclic cyclohexanone compounds, cyclohexene compounds with hydroxyl, phenanthrene ketone compounds with hydroxyl, benzene ring compounds with two long chain esters and cyclohexene compounds with four annular structures.The Aspergillus ochraceus AF-P9 provided by the application provides a strain resource for the prevention and treatment of disease organisms.