162 results about "Pseudomonas syringae" patented technology

The invention discloses a method for producing glucaric acid by constructing recombinant saccharomyces cerevisiae and fermenting and belongs to the technical field of bioengineering. The method comprises the following steps: knocking out an OPI gene of saccharomyces cerevisiae BY4741; introducing myo inositol-1-phosphate synthase and inositol monophosphatase from the saccharomyces cerevisiae, inositol from arabidopsis thaliana and uronic acid dehydrogenase from pseudomonas syringae into the saccharomyces cerevisiae through constitutive plasmid pY26-GPD-TEF, and realizing approach construction of the saccharomyces cerevisiae from glucose to the glucaric acid. According to the method disclosed by the invention, by use of a constructed metabolic pathway, non-selectivity, high energy consumption property and high cost performance of a chemical oxidation method are avoided; the low-priced glucose is directly transformed into D-glucaric acid with high added value by fermentation culture of engineering bacteria; the method has the advantages of mild reaction conditions, high transformation rate and fewer byproducts; production cost can be greatly reduced.

The invention discloses a method for preparing coronatine and a special bacterium thereof. The bacterium provided by the invention is a pseudomonas syringae pv.glycinea with a preservation number of CGMCC No.2533. The bacterium MW123 (with the preservation number of CGMCC No.2533) is an excellent bacterium of a coronatine with high output. Test result shows that the method for using the bacterium MW123 (with the preservation number of CGMCC No.2533) to prepare the coronatine has the advantage of high and stable output; the output can achieve 180mg / L to 200mg / L offermentation broth. Compared with the original bateriusm, the output is improved by 3 to 4 times. Therefore, the method of the invention for preparing the coronatine solves the difficulty of low output of the coronatine of the existing fermentation production and is applicable for industrial popularization and application.

The present invention relates to one kind of genetic engineering bacteria, one kind of soybean disease causing variety of clove pseudomonads with preservation number of CGMCC 1276, and its construction process and use. The genetic engineering bacteria is obtained through inserting Tn5 transposon S17-1 into the genome of the initial strain to deactivate its temperature controlling gene. The strain may be used in industrial fermentation production of crown bacterin at 26-28 deg.c, in the yield near the initial bacteria and up to 35-40 mg / L.

The present invention relates to cotton tissue specific and pathogenic bacterium inducing promoter and their application, and belongs to the field of biotechnology. The promoter contains CAAT-box, TATA-box, ethylene, methyl jasmonate, abscisic acid response element, pathogenic bacterium inducer response elements W boxes, GT-1, MYB, MYBST1, MYB1LEPR, etc. There are also specific root expressing elements to enhance the expression of GUS gene, which expresses specifically in the phloem of root, bud and stem, the vein of foliage and the guard cell of air pore. Paraffin section observation shows that GHNBS promoter expresses in phloem companion cell. After treatment with SA, MeJA, Ethylene, pathogenic bacteria of blight and pseudomonas syringae DC3000, GUS will have obviously raised activity, so that it is one organ specific and pathogenic bacterium relevant promoter.

The invention relates to a method for detecting Pseudomonas syringae causing kiwi canker by loop-mediated isothermal amplification (LAMP), wherein the method comprises the following steps: extracting DNA of Pseudomonas syringae and performing LAMP and real-time turbidity detection. In the LAMP, six primers shown as SEQ ID NO.1-6 are used, and the screening and detection of Pseudomonas syringae causing kiwi canker is at the molecular level, wherein Pseudomonas syringae causing kiwi canker is derived from the Shaanxi Province. The LAMP primers are designed according to the ITS gene of Pseudomonas syringae causing kiwi canker, and the method of detecting Pseudomonas syringae causing kiwi canker is rapid, sensitive and highly-specific by use of the LAMP techniques. The method provides a new approach to rapid detection of Pseudomonas syringae causing kiwi canker.

The invention provides streptomyces erythrochromogenes MO28, which has a preservation number of CGMCC No.3228. The strain is capable of inhibiting the growth of tomato gray mold. Results of panel antibacterial tests show that MO28 strain has high inhibition effect on various plant pathogenic fungi and plant pathogenic bacteria, wherein the fusarium moniliforme inhibition capacity is the highest and the fusarium moniliforme inhibition rate is up to 69.9 percent; and the pseudomonas syringae inhibition diameter is 53.7millimeter. The tomato fruit gray mold prevention and control efficiency of the MO28 is 96.56 percent and the tomato leaf gray mold prevention and control efficiency of the MO28 is 78.26 percent. The strain has no obvious difference from 28-percent chlorothalonil and diethofencarb wettable powder. Results of greenhouse prevention effect detection show that the efficiency of the prevention and control of gray mold on tomatoes, pepper, cucumbers, egg plants and strawberry of the MO28 is between 63 and 77 percent.

The present invention relates to a yeast gene engineering strain capable of expressing hrp gene and its construction method. The name of said strain is Saccharomyces cerevisiae hrp Z, its number is CCTCC No:M203004. Its construction method includes: extraction of pseudomonas syringae genome DNA; using genome DNA as template PCR primer to make construction of hrp Z recombinant yeast gene engineering strain; hrpZ gene contained yeast conversion and induction. The gene engineering harpin protein produced by said strain can induce plant hypersensitive reaction, activate internal self-defense function of plant and promote plant growth. so that it can raise yield and quality of crops.

The invention relates to bacillus subtilis. The preservation number of the strain is CGMCC NO: 9269. The strain is obtained by deep fermentation, ceramic membrane concentration and spray-drying. A shaking culture liquid of the strain has an obvious effect of inhibiting the target germ Xanthomonas oryzae pv. oryzae, the target germ ralstonia solannacearum, the target germ Pseudomonas syringae pv. tabaci, the target germ Xanthomonas axonopodis pv. citri and the target germ cercosporella albomaculans. The bacillus subtilis provided by the invention has advantages of fast growth and propagation, short fermentation period, high filling spore number, good control efficiency and the like. Meanwhile, a preparation method of the strain has simple steps, is convenient to operate, comprises steps of deep fermentation, ceramic membrane concentration and spray-drying to obtain the bacillus subtilis, has high preparation efficiency and has good effects. The strain can control various bacterial diseases and now has been registered on cercosporella albomaculans, Xanthomonas axonopodis pv. citri, Pseudomonas syringae pv. tabaci, ralstonia solannacearum and Xanthomonas oryzae pv. oryzae.

A strain capable of producing highly component crown bacterin by fermentation is disclosed. The strain, Y-2018 strain of Psendomonas syringae, is preserved by the number CGMCC NO.1105. The strain has no need to culture under low temperature, conquers the defects that wild strains has to accumulate crown bacterin under low temperature. The invention also provides a method for mass production of crown bacterin, which comprises the steps: bevel strain preparation, bottle rocking to culture, aerating and fermenting to obtain fermentation liquor containing crown bacterin, then concentrating, extracting, crystallizing, recrystallizing to obtain crystalline flour and non-crystal mother liquor. The method can employ the organic nitrogen culture medium to ferment, which can not only increase the bacteria quantity, but also can synthesize more crown bacterin, thus conquers the defect that wild strain can not endure organic nitrogen.

The present invention relates to gene engineering bacterial strain, and is especially one kind of soybean disease causing variety (P.syringae pv.Glycinea) of pseudomonas syringae for producing crown bacterin and its construction process. The gene engineering pseudomonas strain is prepared through mutagenizing initial pseudomonas strain with nitrosoguanidine for genetic mutation, so as to screen out pseudomonas strain with high crown bacterin yield. Experiment proves that the gene engineering pseudomonas strain of the present invention may be used in industrial production of crown bacterin through fermentation at 18deg.c to reach the yield of 84.7-112 mg / L.

The invention relates to a pseudomonas syringae, a screening method thereof and application to kill nematode. The classification name of the pseudomonas syringae is pseudomonas syringae MB03, colonies has protuberances and show milk white, the thalline is in the shape of a rod through microscopic examination and belongs to a gram-negative bacterium. The screening method comprises: acquiring frozen plant tissue, chopping and culturing in a medium, and finally picking growing single colony, and performing separation purification through streaking. The application to kill nematode means that the thalline, a fermentation supernatant, a cell disruption solution, or extracellular crude protein and / or intracellular crude protein of pseudomonas syringae MB03 subjected to ammonium sulfate precipitation is used to kill caenorhabditis elegans and meloidogyne incognita. The advantages comprise that pseudomonas syringae is low in production cost and free of pollution to environment, and has relatively killing effect on caenorhabditis elegans and meloidogyne incognita. The factor, killing nematode, in pseudomonas syringae is protein, so that pseudomonas syringae is sutiable for molecular gene operation, and has great value on microbe control resource and mechanism research.

The invention relates to pseudomonas sp. named ZY-1 and a function of the metabolite thereof in curing plant diseases, wherein the microbial collection number of the pseudomonas syringae is CGMCC (China General Microbiological Culture Collection Center) No.7906, the collection date is July 10, 2013, and the collection institution is the CGMCC. The pseudomonas syringae provided by the invention has the advantages of obvious efficacy, short curing time, no environmental pollution, simplicity and convenience in operation and the like.

The invention discloses a new Pseudomonas corrugate strain (P94 GMCC No.1895), which is characterized by the following: manufacturing relative HCN to prevent bacteria, proteinase and phospholipase and IAA; inhibiting partial plant pathogenic fungus (Botrytis cinerea, Ceratocystis fimbriata, Monilinia laxa, Magnaporthe grisea, Pythium aphanidermatum and Phytophthora capsici) and partial pathogenic bacteria (Pseudomonas syringae, Acidovorax avenae and Ralstonia solanacearum); accelerating the growth of tomato and cucumber to improve production by 22%-28%.

The invention discloses aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and an application of aspergillus tubingensis metabolites. The bacterial strain No. of aspergillus tubingensis is QF05, and the registration No. of aspergillus tubingensis in CGMCC (China general microbiological culture collection center) is CGMCC No.13563. Aspergillus tubingensis QF05 has an inhibition function on botrytis cinerea, colletotrichum capsici, fusarium wilt of cucumbers, alternaria solani and pseudomonas syringae pv.lachrymans. Sterile fermentation filtrate of aspergillus tubingensis QF05 can remarkably increase the germination rate of pepper seeds and promote elongation of radicle. Aspergillus tubingensis QF05 metabolites containing bacteria effectively promote growth of true leaves of cucumbers and effectively increase the plant height and the yield of the cucumbers.

The invention discloses a real-time fluorescence PCR method for quantitatively detecting Pseudomonas syringae pv. Actinidiae. The method comprises the following steps: extracting an Actinidia chinensis sample genome DNA; electrophoretically detecting the band size and purity of the Actinidia chinensis sample genome DNA; carrying out fluorescent quantitative detection on a PSA pathogen; carrying out specific primer-based fluorescent quantitative amplification, and preliminarily determining whether the PSA pathogen is contained or not according to an amplification curve; purifying a PCR product with an obvious amplification peak, and sequencing the purified PCR product; and comparing a sequence obtained after the sequencing by Internet to further determine the PSA pathogen. The method allows a fluorescent quantitative PCR-based rapid detection method of the pathogen to be established, realizes rapid identification of the Pseudomonas syringae pv. Actinidiae infected sample, has the characteristics of simplicity in operation, high sensitivity, high specificity, and accurate and objective detection result, and is of great significance to improve the detection efficiency and the detection sensitivity of the Pseudomonas syringae pv. Actinidiae infected sample.

The invention discloses a pure plant preparation for preventing and treating plant canker diseases. The pure plant preparation is prepared by the following materials according to certain weight part: wild-mountainous tea, white birch leaves, walnut green seedcase, mountain elm leaves, pokeberry root, affine cudweed, calamus, humifuse euphorbia herb, nepeta yanthina, white pepper and wild bee honey. The invention also discloses a preparation method of the pure plant preparation. The preparation method comprises the following specific steps: weighing all materials according to the specified ratio, and respectively removing impurities in all the materials except the wild bee honey; respectively disinfecting all the materials except the wild bee honey, respectively juicing by high-temperature soaking, and carrying out monomer reaction on all the obtained single juices; fully mixing all the juices and the wild bee honey, sieving, purifying, and standing for mixing reaction; and stirring the mixed juices uniformly, and carrying out packet package to obtain the preparation. The pure plant preparation disclosed by the invention has an obvious preventing and treating effect for the plant canker diseases caused by infection of pseudomonas syringae, has no influence on plant products, has no pollution to the environment, and is an ideal medicament for preventing and treating the plant canker diseases.

The invention relates to an application of an isoflavonoids compound, that is, glabridin, to prepare an agricultural bactericide. The compound is used for inhibiting the following pathogenic funguses of crops: fusarium graminearum, colletotrichum gloeosporioides, botrytis cinerea, phytophthora infestans, or pyricularia grisea, and the following pathogenic bacteria of crops: erwina carotovora, pseudomonas solanacearum, ralstonia solanacearum, or pseudomonas syringae pv. actinidae. Furthermore, the compound can be prepared into various formulations with other agricultural auxiliaries, and the compound has a synergistic effect after combined with shikonin according to a certain weight ratio.

The present invention discloses applications of linoleoylethanolamine in improvement of gray mold resistance and bacterial leaf spot resistance of plants, and in preparation of plant gray mold and / or bacterial leaf spot resistance improving preparations. According to the present invention, linoleoylethanolamine is adopted as a main active ingredient to prepare the preparation, and the signal paths of jasmonic acid, salicylic acid and ethylene in plants are induced so as to significantly enhance the gray mold resistance and the bacterial leaf spot resistance of the plant, and reduce the economic loss of the gray mold and the bacterial leaf spot on the plant; and with the application of the preparation to prevent and control the gray mold and the bacterial leaf spot, the advantages of simpleness, easy performing and low cost are provided, the growth of the single or compound pathogenic bacteria of Botrytis cinerea and Pseudomonas syringae on the leaves and the plant disease diffusion can be significantly delayed and inhibited, and the gray mold resistance and the bacterial leaf spot resistance of the plant are substantially improved.

The invention relates to a nucleic acid sequence that improves resistance to biotrophic pathogens, in particular to Pseudomonas syringae, without affecting susceptibility to necrotrophs, in particular Botrytis cinerea. The present invention also relates to a plant that comprises the nucleic acid of the invention and a method to generate plants resistant to biotrophic pathogens and necrotrophs.

Stable genetically engineered bacterial strains that overproduce coronatine are provided. The stable strains can be successfully cultivated to overproduce coronatine at temperatures that are suitable for large scale, commercial preparations of coronatine. The overproducing strains are also non-pathogenic. An exemplary strain is Pseudomonas syringae APV1, which successfully overproduces coronatine at 26° C. Methods of optimizing culture conditions for coronatine production from the novel stable overproducing strains are provided, as are methods for using the overproducing strains to induce abscission and increase taxane production.

The invention discloses rice seedling nursing soil, as well as a preparation method and application thereof, and relates to the field of rice seedling nursing. The problems of straw combustion and difficulty in soil taking during rice seedling nursing in spring are solved. The rice seedling nursing soil is prepared from the following components in parts by weight: 100 to 200 parts of soil, 80 to 90 parts of straw powder, 10 to 20 parts of a compound microbial agent, 0.1 to 0.15 part of potassium dihydrogen phosphate, 0.1 to 0.4 part of ammonium sulfate and 0.1 to 0.3 part of quicklime, wherein the compound microbial agent is prepared from the following components: pseudomonas syringae, bacillus thuringiensis, bacillus subtilis, bacillus licheniformis, trichoderma and lactobacillus plantarum, and the mass ratio of the pseudomonas syringae, the bacillus thuringiensis, the bacillus subtilis, the bacillus licheniformis, the trichoderma and the lactobacillus plantarum is 1.5:1:1:1:1:1.5. According to the rice seedling nursing soil and the preparation method and application thereof, insect killing, sterilization, pest and disease damage prevention and control and increase of transmission of oxygen in the soil can be simultaneously implemented, growth and reproduction of microorganisms are facilitated, pollution is avoided, and the yield is high.

The invention discloses a carboxylesterase D-1CarE3 cell surface display system and a whole-cell catalyst as well as a preparation method and an application of the whole-cell catalyst. A construction method of the surface display system comprises the following steps: constructing a prokaryotic expression vector pET28NCD3 by utilizing an NC end of synthetic truncated ice nucleoprotein of source pseudomonas syringae as ankyrin to be fused with target protein carboxylesterase D-1CarE3, and converting the prokaryotic expression vector into escherichia coli BL21 to construct escherichia coli cell surface display engineering bacterium BL21 / pET28NCD3 of the carboxylesterase D-1CarE3. Under the effect of a strong promoter T7, through IPTG inducible expression, the display of carboxylesterase D-1CarE3 cell surface activity is successfully realized, and the whole-cell catalyst D-1CarE3 is prepared by using an ultra-low temperature freeze drying method. The whole-cell catalyst D-1CarE3 can be applied to the treatment of environmental pollution of pesticide residues.

The invention discloses myxobacteria and applications thereof. The myxobacteria M34 is assigned the accession number GDMCC No: 61026. The myxobacteria M34 can obviously inhibit the growth of plant pathogenic fungi and includes Rhizoctonia solani Kuhn causing rice sheath blight and Fusarium oxysporum f.sp.cubense race 4 and Colletotrchum musarum Cooke et Mass causing banana vasicular wilt, and canprey on Pseudomonas syringae GIM 1.330, Ralstonia solanacearum GIM 1.70, Xanthomonas campestris JCM 20466 and Curtobacterium flaccumfaciens GIM 1.343. The strain has broad-spectrum inhibition effectson plant pathogens, is various in inhibition strategy including the direct inhibition of fungal growth and the predation of bacteria and has good application values on preparing Microbial fertilizer additives and biocontrol bacterial agents.

The invention relates to an application of a host defense inducer in preventing and treating selected bacteria pests in useful plants, wherein the selected bacteria pests are selected from acidovoraxavenae, burkholderia species, burkholderia glumae, phloem bacillus species, corynebacterium, erwinia species, pseudomonas syringae, pathogenic variant of pseudomonas syringa kiwi, pathogenic variant of pseudomonas syringe soybean, pathogenic variant of pseudomonas syringe tomato, pathogenic variant of pseudomonas syringe cucumber, streptomyces species, xanthomonas species, xanthomonas axonopodis,pathogenic variant of xanthomonas axonopodis citrus, pathogenic variant of xanthomonas axonopodis soybean, xanthomonas campestris, pathogenic variant of xanthomonas campestris banana, pathogenic variant of xanthomonas campestris peaches and plums, strawberry xanthomonas and semi-transparent xanthomonas. The invention also relates to a method for preventing and treating selected bacteria pests in useful plants by adopting the host defense inducer for treatment.

The invention discloses a method for screening pseudomonas syringae pv.tabaci antagonistic bacteria, which is characterized in that an improved asynchronism opposing culture method is adopted, bacteria to be tested are in point inoculum of 4-6 points and are cultured for 2-3 days, pseudomonas syringae pv.tabaci suspension is sprayed and dispersed, the bacteria are continuously cultured in an opposing way for 2-3 days after the water on the surface is dried in a sterile way and are observed for inhibition zones, and the bacteria with the inhibition zones are target bacteria obtained after the screening. The invention can remarkably enhance the screening efficiency, enrich the asynchronism opposing culture method and accelerate the biological control of bacterial plant diseases.

One aspect of the present invention relates to isolated nucleic acid molecules encoding avirulence proteins or polypeptides of Pseudomonas syringae pv. syringae DC 3000, or nucleic acid molecules which are complementary thereto. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The various nucleic acid molecules and proteins of the present invention can be used to impart disease resistance to a plant, make a plant hypersusceptible to colonization by nonpathogenic bacteria, modify a metabolic pathway in a cell, cause eukaryotic cell death and treat a cancerous condition, as well as inhibit programmed cell death.

The present invention relates to isolated DNA molecules that encode proteins or polypeptides which elicit a hypersensitive response in plants. One aspect of the present invention involves an isolated DNA molecule that encodes the HopPtoP protein of Pseudomonas syringae pv. tomato DC3000. The isolated DNA molecules can be used to impart disease resistance, stress resistance, and enhanced growth to plants or plants grown from treated seeds, to control insects on plants or plants grown from treated plant seeds, to impart post-harvest disease or desiccation resistance in fruits or vegetables, to impart enhanced longevity of fruit or vegetable ripeness, to impart desiccation resistance to cuttings of ornamental plants, and / or promote early flowering of ornamental plants, either by topical application of the proteins or polypeptides or transgenic expression in recombinant plants or plant seeds. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are disclosed.

The invention provides a method for detecting Psa in kiwi fruit pollens. The method comprises the following steps: extracting DNA from pollen mixed bacterial suspension; designing two pairs of specific primers according to hrpW gene of kiwi fruit Psa, carrying out nested-PCR, amplifying a pollen sample to obtain an outer primer amplified band with a size of about 590 bp and an inner primer amplified band with a size of about 240 bp; and finally carrying out sequencing to verify the Psa. The detection sensitivity is 103 cfu / mL. The provided Psa rapid detection method has the advantages of stronger specificity, higher stability, and higher sensitivity, can differentiate pathogenic bacteria having close ties of consanguinity with Psa such as pseudomonas syringae pv.Theae, pseudomonas avellanae, and the like, and is capable of reducing the false positive results.