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Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites

A technology of Aspergillus tabin and metabolites, which is applied in the field of preparation of Aspergillus tabin and its metabolites, can solve the problems of biodiversity destruction, decreased control effect, environmental pollution and the like

Active Publication Date: 2017-04-26
北京奥沃伽科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of a single type of fungicide and unreasonable application techniques lead to resistance of pathogenic bacteria, and the control effect decreases year by year. At the same time, it brings a series of problems such as excessive chemical pesticide residues, environmental pollution, and destruction of biodiversity.

Method used

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  • Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites
  • Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites
  • Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1, isolation and identification of Aspergillus tubingensis QF05

[0082] 1.1 Strain isolation

[0083] The strain was isolated from soil samples in special habitats of Qinghai-Tibet Plateau, Qinghai Province, China. Bacterial strains were separated by the dilution plate method, and the specific operation method was as follows: Weigh 10 g of the soil sample and put it into 90 ml of sterile water to vibrate evenly to obtain 10 -1 Concentration of bacterial solution, use a 1ml sterile pipette to draw 1ml 10 -1 Concentration of bacterial solution in a tube of 9ml sterile water, shake well that is 10 -2 Concentration of bacterial solution, the same method, sequentially diluted to 10 -4 . the above 10 -2 、10 -3 and 10 -4 Spread the soil bacteria solution on the PDA plate respectively, dry it and place it upside down in a 25°C incubator for 3-5 days. After the isolated fungal single colony is purified, transfer it to a PDA slant for culture. After it grows we...

Embodiment 2

[0094] Embodiment 2, Aspergillus tubingensis (Aspergillus tubingensis) QF05 is to the competitive inhibitory effect of testing pathogenic bacteria

[0095] Pick the Aspergillus tubingensis (Aspergillus tubingensis) QF05 bacterium cake with a diameter of 7mm grown on the flat plate and the bacteria cake of the pathogenic bacteria for testing, and inoculate them symmetrically on the PDA medium respectively at a position 2cm from the center, and the control does not inoculate Aspergillus tubingensis (Aspergillus tubingensis) tubingensis) QF05 bacterium cake, only inoculate the test pathogen bacterium cake, each treatment was repeated three times, and cultured at 25°C for 4 days to observe the growth of the pathogen. The tested pathogens include: Botrytis cinerea, Fusarium moniliforme, Colletotrichum capsici, Fusarium oxysporum f.sp.cucumerinum, Rhizoctonia cereali), Alternaria tenuissima, Fusarium oxysporum f. sp. conglutinans, Monilinia fructicola.

[0096] The results showed t...

Embodiment 3

[0100] Embodiment 3, Aspergillus tubingensis (Aspergillus tubingensis) QF05 aseptic fermentation filtrate is to the inhibitory effect of testing pathogenic bacteria

[0101] 1. Preparation of Aspergillus tubingensis QF05 sterile fermentation filtrate

[0102] Pick up the Aspergillus tubingensis QF05 grown on the plate with the inoculation loop and inoculate it into a 500mL Erlenmeyer flask containing 100mL of liquid fermentation medium, culture it on a shaker at 150rpm at 25°C for 4 days, collect the fermentation broth, and use a 0.22μm micropore Filtering the fermentation liquid with a filter membrane to remove Aspergillus tubingensis QF05 cells in the culture to obtain Aspergillus tubingensis QF05 sterile fermentation filtrate.

[0103] Among them, the liquid fermentation medium is maltose 20g, glycerin 20ml, beef extract 30g, peptone 30g, distilled water to 1000mL, sterilized at 121°C for 20min to obtain the liquid fermentation medium.

[0104] 2. Antibacterial effect

[...

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Abstract

The invention discloses aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and an application of aspergillus tubingensis metabolites. The bacterial strain No. of aspergillus tubingensis is QF05, and the registration No. of aspergillus tubingensis in CGMCC (China general microbiological culture collection center) is CGMCC No.13563. Aspergillus tubingensis QF05 has an inhibition function on botrytis cinerea, colletotrichum capsici, fusarium wilt of cucumbers, alternaria solani and pseudomonas syringae pv.lachrymans. Sterile fermentation filtrate of aspergillus tubingensis QF05 can remarkably increase the germination rate of pepper seeds and promote elongation of radicle. Aspergillus tubingensis QF05 metabolites containing bacteria effectively promote growth of true leaves of cucumbers and effectively increase the plant height and the yield of the cucumbers.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to the preparation and application of a disease-preventing and growth-promoting Aspergillus tubingensis and its metabolites. Background technique [0002] With the development of horticultural facility cultivation and the expansion of planting area in protected areas, diseases of fruit and vegetable crops are common and cause serious economic losses to people's production and life. At present, domestic and foreign research on disease control of fruit and vegetable crops is mainly based on comprehensive control from the aspects of breeding excellent disease-resistant varieties, seed disinfection treatment, strengthening cultivation management measures, spraying chemical agents, etc., and chemical control is the main method. Long-term use of a single type of fungicide and unreasonable application techniques lead to the development of resistance of pathogenic b...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P21/00A01P1/00C12R1/66
CPCC12N1/14A01N63/30
Inventor 刘德文赵娟刘伟成卢彩鸽吴慧玲张殿朋刘霆董丹张涛涛田兆丰
Owner 北京奥沃伽科技有限公司
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